AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0096870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0096870 MtrunA17_Chr1g0153261 79.394 165 3 2 1 134 1 165 3.62e-77 232
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0096870 AT1G45976.1 74.312 109 24 2 30 134 49 157 1.41e-50 164

Find 94 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAGCATCTTATTGGTTTA+TGG 0.162223 1_3:-2589987 Msa0096870:exon
TGTCTAGTTTAGCATCTTAT+TGG 0.233889 1_3:-2589994 Msa0096870:exon
TTAGCATCTTATTGGTTTAT+GGG 0.294043 1_3:-2589986 Msa0096870:exon
TTTCATCAAGTCTTTCATAT+TGG 0.300589 1_3:+2588992 None:intergenic
TTCTTCATAGATGCTAAATT+AGG 0.301455 1_3:+2589433 None:intergenic
AACCACAAACCAAATCTTTC+AGG 0.323593 1_3:-2591103 Msa0096870:intron
GTTGAGGTTGTGGTTGTTGC+TGG 0.326770 1_3:+2591123 None:intergenic
GTTGATTTGCATTGGAATTT+CGG 0.328434 1_3:-2590270 Msa0096870:CDS
CGATCTGTGTCCACGGGGTT+AGG 0.331116 1_3:-2590162 Msa0096870:CDS
AAACATCAACAAGAGGAATA+TGG 0.343288 1_3:-2589237 Msa0096870:three_prime_UTR
CTCCATGCCGATGAATTTAG+AGG 0.351654 1_3:+2588860 None:intergenic
TGTGTAAATATTAAATAATA+TGG 0.355988 1_3:+2589881 None:intergenic
AAAGATTTGGTTTGTGGTTG+AGG 0.373807 1_3:+2591107 None:intergenic
TAGTAATTACCTGAAAGATT+TGG 0.385861 1_3:+2591094 None:intergenic
TCGGCTTTATTGTCTCTCAT+AGG 0.391703 1_3:-2590099 Msa0096870:CDS
TGCTCCGGGTCCTGTCATTC+CGG 0.393035 1_3:-2590313 Msa0096870:CDS
CATGGAGGTCTACATGTAAC+TGG 0.398646 1_3:-2588844 Msa0096870:three_prime_UTR
AATTCATCATGATCGGTAAC+TGG 0.405213 1_3:-2589905 Msa0096870:three_prime_UTR
GCTGGTAGTGGCGTTGAAAC+TGG 0.406582 1_3:+2591141 None:intergenic
TTGGTTTGTGGTTGAGGTTG+TGG 0.413733 1_3:+2591113 None:intergenic
CGGCTTTATTGTCTCTCATA+GGG 0.421365 1_3:-2590098 Msa0096870:CDS
AAAAGCCATGGAGGAGGAGG+AGG 0.422303 1_3:+2591172 None:intergenic
CTCAAACTGCAGGTTAGCTC+AGG 0.432077 1_3:-2590024 Msa0096870:CDS
AACACTCGCCTGGCTTCAAC+AGG 0.433505 1_3:-2590126 Msa0096870:CDS
TATGGAACTTGAAGATCAAA+TGG 0.436027 1_3:-2589219 Msa0096870:three_prime_UTR
GAGGAAAAGACTAAAAGAAC+AGG 0.439039 1_3:-2590235 Msa0096870:CDS
TTTGCATTGGAATTTCGGTT+TGG 0.453573 1_3:-2590265 Msa0096870:CDS
CTTCTTTACTGTCCCTACCT+TGG 0.456069 1_3:+2589099 None:intergenic
GAAAGACTTGATGAAATGTA+AGG 0.468795 1_3:-2588985 Msa0096870:three_prime_UTR
GGTCCTGTCATTCCGGCTGA+TGG 0.469575 1_3:-2590306 Msa0096870:CDS
CAACCTCGATCTGTGTCCAC+GGG 0.471862 1_3:-2590168 Msa0096870:CDS
GATACAGCTTCTTGCTGTAA+TGG 0.478491 1_3:-2589053 Msa0096870:three_prime_UTR
GTTGTGGTTGTTGCTGGTAG+TGG 0.484704 1_3:+2591129 None:intergenic
AAGAAAAGCCATGGAGGAGG+AGG 0.486504 1_3:+2591169 None:intergenic
TACCTGAAAGATTTGGTTTG+TGG 0.491471 1_3:+2591101 None:intergenic
CTTCCATCAGCCGGAATGAC+AGG 0.496376 1_3:+2590303 None:intergenic
CAAGGTAGGGACAGTAAAGA+AGG 0.497061 1_3:-2589098 Msa0096870:three_prime_UTR
TGATCGTGAGTTACAGCAGC+AGG 0.505797 1_3:-2590067 Msa0096870:CDS
GCAACCTCGATCTGTGTCCA+CGG 0.508164 1_3:-2590169 Msa0096870:CDS
AATTTAGAGGATTGACATAG+AGG 0.508849 1_3:+2588873 None:intergenic
TCTCAAGTTTAACCTCCAAC+AGG 0.509968 1_3:-2589129 Msa0096870:three_prime_UTR
ATGGTGGTGTTGATTTGCAT+TGG 0.512381 1_3:-2590278 Msa0096870:CDS
CTCTTACGAGTACAATATAC+AGG 0.513282 1_3:+2589456 None:intergenic
CCGAGTCTCCTGTTGAAGCC+AGG 0.516637 1_3:+2590118 None:intergenic
GTCTCTCGAGGGAATGTGAA+TGG 0.518733 1_3:+2591224 None:intergenic
AAAACTTCGCGAGAAAGAAA+CGG 0.522431 1_3:-2589267 Msa0096870:three_prime_UTR
GTCGCTCGATAACACTCGCC+TGG 0.522524 1_3:-2590136 Msa0096870:CDS
GGACAGATTTCTCAAACTGC+AGG 0.527660 1_3:-2590034 Msa0096870:CDS
TACCTTGGAGATATGCCTGT+TGG 0.532431 1_3:+2589114 None:intergenic
ATCCTCTAAATTCATCGGCA+TGG 0.537187 1_3:-2588862 Msa0096870:three_prime_UTR
CTTGGAGATATGCCTGTTGG+AGG 0.541227 1_3:+2589117 None:intergenic
AAATGTAAATTCATCATGAT+CGG 0.546889 1_3:-2589912 Msa0096870:three_prime_UTR
GGTTAGAATGCATCACAATA+CGG 0.546930 1_3:+2589477 None:intergenic
CAGAGGCAAAGATGCTTGCA+AGG 0.548296 1_3:+2588927 None:intergenic
AAAGGAAAGAGGGGTAAGTG+TGG 0.548972 1_3:-2591283 None:intergenic
TGTCAATCCTCTAAATTCAT+CGG 0.564430 1_3:-2588867 Msa0096870:three_prime_UTR
AGGGACAGTAAAGAAGGGTG+TGG 0.567717 1_3:-2589092 Msa0096870:three_prime_UTR
CCTGGCTTCAACAGGAGACT+CGG 0.567720 1_3:-2590118 Msa0096870:CDS
TGATTTGTGAAATAATCGAT+TGG 0.569293 1_3:+2589571 None:intergenic
CCACCATCACTTCCATCAGC+CGG 0.574524 1_3:+2590294 None:intergenic
AACAGGCATATCTCCAAGGT+AGG 0.579800 1_3:-2589112 Msa0096870:three_prime_UTR
TAATTCTCAGATATCTTCTG+TGG 0.580087 1_3:-2590199 Msa0096870:CDS
TCAGCCGGAATGACAGGACC+CGG 0.584748 1_3:+2590309 None:intergenic
GTATGCTGATGATTTGTAAG+TGG 0.586429 1_3:-2589845 Msa0096870:three_prime_UTR
TTGAAGAAAAGCCATGGAGG+AGG 0.587815 1_3:+2591166 None:intergenic
CTGGTCTTGAAGAAAAGCCA+TGG 0.589502 1_3:+2591160 None:intergenic
GAACAGCTAAGTGTAGAAGC+AGG 0.592248 1_3:-2589197 Msa0096870:three_prime_UTR
TCTAATAGGGTGAACAATTG+CGG 0.596973 1_3:-2589361 Msa0096870:three_prime_UTR
ACAGCAGCAGGATTTAGAGA+TGG 0.603378 1_3:-2590055 Msa0096870:CDS
TAAGTGTAGAAGCAGGCGCA+TGG 0.605128 1_3:-2589190 Msa0096870:three_prime_UTR
TGTTTCAATCATTGAAGACA+AGG 0.609124 1_3:-2589297 Msa0096870:three_prime_UTR
CAGAAGAGAGAAGTCTCTCG+AGG 0.610039 1_3:+2591212 None:intergenic
CTCCAACAGGCATATCTCCA+AGG 0.619146 1_3:-2589116 Msa0096870:three_prime_UTR
AGAAGAGAGAAGTCTCTCGA+GGG 0.619491 1_3:+2591213 None:intergenic
TACTTTCACAATCTTTACAG+AGG 0.619763 1_3:+2588910 None:intergenic
ACAGGCATATCTCCAAGGTA+GGG 0.623265 1_3:-2589111 Msa0096870:three_prime_UTR
CCGGCTGATGGAAGTGATGG+TGG 0.631778 1_3:-2590294 Msa0096870:CDS
AGGTAGAAAACATCAACAAG+AGG 0.632388 1_3:-2589244 Msa0096870:three_prime_UTR
CAGAGTCAACGAAGTGACGA+TGG 0.636128 1_3:-2588958 Msa0096870:three_prime_UTR
CTCTAAATTCATCGGCATGG+AGG 0.640451 1_3:-2588859 Msa0096870:three_prime_UTR
ATTCCGGCTGATGGAAGTGA+TGG 0.641493 1_3:-2590297 Msa0096870:CDS
GGCTTTATTGTCTCTCATAG+GGG 0.643215 1_3:-2590097 Msa0096870:CDS
AAGGTAGGGACAGTAAAGAA+GGG 0.646022 1_3:-2589097 Msa0096870:three_prime_UTR
AAACTAGTTACAATGTGTCA+CGG 0.649683 1_3:+2588709 None:intergenic
TGTCACGGTACTTGTTACAA+TGG 0.651312 1_3:+2588724 None:intergenic
AGGGAATGTGAATGGCGATG+AGG 0.665502 1_3:+2591232 None:intergenic
AACCCCGTGGACACAGATCG+AGG 0.671438 1_3:+2590165 None:intergenic
ATTTCGGTTTGGAACCAGAG+AGG 0.674088 1_3:-2590254 Msa0096870:CDS
GTCTTGAAGAAAAGCCATGG+AGG 0.676482 1_3:+2591163 None:intergenic
AACCTCGATCTGTGTCCACG+GGG 0.677662 1_3:-2590167 Msa0096870:CDS
ACTATGATAAGCAACAACAC+AGG 0.683625 1_3:+2588769 None:intergenic
GAGCGACAATCCTAACCCCG+TGG 0.690626 1_3:+2590152 None:intergenic
AGAAGGGTGTGGTGACAGCG+AGG 0.695907 1_3:-2589081 Msa0096870:three_prime_UTR
ACTTCGCGAGAAAGAAACGG+AGG 0.727027 1_3:-2589264 Msa0096870:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGAAAAATATGAATTTTT+AGG - chr1_3:2589187-2589206 Msa0096870:three_prime_UTR 10.0%
!!! TATTTTGTTTTTGATTTTTT+TGG - chr1_3:2589544-2589563 Msa0096870:three_prime_UTR 10.0%
!!! ATTTTGTTTTTGATTTTTTT+GGG - chr1_3:2589545-2589564 Msa0096870:three_prime_UTR 10.0%
!! TGTGTAAATATTAAATAATA+TGG + chr1_3:2590026-2590045 None:intergenic 10.0%
!!! ATTATTTTGGAATATTAAGA+GGG + chr1_3:2588843-2588862 None:intergenic 15.0%
!!! AATTATTTTGGAATATTAAG+AGG + chr1_3:2588844-2588863 None:intergenic 15.0%
!!! CACTAATCTTAATTAATTTT+TGG - chr1_3:2588960-2588979 Msa0096870:three_prime_UTR 15.0%
!!! ATATGAATTTTTAGGTTTTT+TGG - chr1_3:2589195-2589214 Msa0096870:three_prime_UTR 15.0%
!! TTAATTCAAAATAACCAAAA+GGG + chr1_3:2589361-2589380 None:intergenic 15.0%
!! TTTAATTCAAAATAACCAAA+AGG + chr1_3:2589362-2589381 None:intergenic 15.0%
!!! TTTTGTTTTTGATTTTTTTG+GGG - chr1_3:2589546-2589565 Msa0096870:three_prime_UTR 15.0%
!!! TAATTAATTTTCTCTCTAAT+AGG - chr1_3:2590529-2590548 Msa0096870:intron 15.0%
!!! AATTAATTTTCTCTCTAATA+GGG - chr1_3:2590530-2590549 Msa0096870:intron 15.0%
!!! TATTCAGTTTTAGTAAATTT+TGG - chr1_3:2591255-2591274 Msa0096870:five_prime_UTR 15.0%
!!! TTATTTTGGAATATTAAGAG+GGG + chr1_3:2588842-2588861 None:intergenic 20.0%
!!! GAAAGAAATTGGAATTATTT+TGG + chr1_3:2588856-2588875 None:intergenic 20.0%
!! GAGAGATTAAAAAAAATGTT+TGG + chr1_3:2588893-2588912 None:intergenic 20.0%
!! AGTGAGAAATAGATAATTAA+AGG + chr1_3:2588944-2588963 None:intergenic 20.0%
!! AGAAATTTACAAACAATTGA+AGG - chr1_3:2588991-2589010 Msa0096870:three_prime_UTR 20.0%
!!! GTCTATTTAGTATTTTCTTT+AGG - chr1_3:2589304-2589323 Msa0096870:three_prime_UTR 20.0%
!!! ATTTTCTTTAGGTTTTTCTT+AGG - chr1_3:2589315-2589334 Msa0096870:three_prime_UTR 20.0%
!!! TTTTCTTTAGGTTTTTCTTA+GGG - chr1_3:2589316-2589335 Msa0096870:three_prime_UTR 20.0%
!!! TTTCTTTAGCATTTTATTGA+AGG - chr1_3:2589456-2589475 Msa0096870:three_prime_UTR 20.0%
!!! TTCTTTAGCATTTTATTGAA+GGG - chr1_3:2589457-2589476 Msa0096870:three_prime_UTR 20.0%
!!! GGGTTTTTAGTTTTAATTTT+GGG - chr1_3:2589478-2589497 Msa0096870:three_prime_UTR 20.0%
!!! TTTGTTTTTGATTTTTTTGG+GGG - chr1_3:2589547-2589566 Msa0096870:three_prime_UTR 20.0%
!! AAATGTAAATTCATCATGAT+CGG - chr1_3:2589992-2590011 Msa0096870:exon 20.0%
!!! TTTCATATTGGAATTTTCAT+TGG + chr1_3:2590903-2590922 None:intergenic 20.0%
!!! AACTGGATATTTTATTTCAA+CGG - chr1_3:2591077-2591096 Msa0096870:intron 20.0%
! TAGTAATTACCTGAAAGATT+TGG + chr1_3:2588813-2588832 None:intergenic 25.0%
! AAAATGTTTGGTAAAGTGAA+AGG + chr1_3:2588881-2588900 None:intergenic 25.0%
! AAATCATTCTACAACACAAA+AGG + chr1_3:2589257-2589276 None:intergenic 25.0%
!!! TTTCTTTAGGTTTTTCTTAG+GGG - chr1_3:2589317-2589336 Msa0096870:three_prime_UTR 25.0%
!! TCTTTAGCATTTTATTGAAG+GGG - chr1_3:2589458-2589477 Msa0096870:three_prime_UTR 25.0%
!!! GGGGTTTTTAGTTTTAATTT+TGG - chr1_3:2589477-2589496 Msa0096870:three_prime_UTR 25.0%
!!! ACTAAAAGAACAGGATTTTT+TGG - chr1_3:2589678-2589697 Msa0096870:three_prime_UTR 25.0%
!!! TTTAGCATCTTATTGGTTTA+TGG - chr1_3:2589917-2589936 Msa0096870:three_prime_UTR 25.0%
!!! TTAGCATCTTATTGGTTTAT+GGG - chr1_3:2589918-2589937 Msa0096870:three_prime_UTR 25.0%
!! TGATTTGTGAAATAATCGAT+TGG + chr1_3:2590336-2590355 None:intergenic 25.0%
! TTCTTCATAGATGCTAAATT+AGG + chr1_3:2590474-2590493 None:intergenic 25.0%
!!! AACAATTTTAGAGAAAGTTC+AGG - chr1_3:2590568-2590587 Msa0096870:intron 25.0%
!! AATCATGTTTTCGTTATATC+TGG + chr1_3:2590750-2590769 None:intergenic 25.0%
! TTTCATCAAGTCTTTCATAT+TGG + chr1_3:2590915-2590934 None:intergenic 25.0%
!!! CAGTTTTAGTAAATTTTGGA+AGG - chr1_3:2591259-2591278 Msa0096870:five_prime_UTR 25.0%
AGTGAAAGGTAGAAAGAAAT+TGG + chr1_3:2588867-2588886 None:intergenic 30.0%
AGATGAAGAATTTGCATACA+TGG + chr1_3:2589093-2589112 None:intergenic 30.0%
TTGACTCAAAATGACCAAAA+GGG + chr1_3:2589227-2589246 None:intergenic 30.0%
TTTGACTCAAAATGACCAAA+AGG + chr1_3:2589228-2589247 None:intergenic 30.0%
TCACAACACATGAAAATGTA+GGG + chr1_3:2589523-2589542 None:intergenic 30.0%
!! GTTGATTTGCATTGGAATTT+CGG - chr1_3:2589634-2589653 Msa0096870:three_prime_UTR 30.0%
TAATTCTCAGATATCTTCTG+TGG - chr1_3:2589705-2589724 Msa0096870:three_prime_UTR 30.0%
! TGTCTAGTTTAGCATCTTAT+TGG - chr1_3:2589910-2589929 Msa0096870:three_prime_UTR 30.0%
!! TTTTTGTGCTTGTTAGACAA+TGG - chr1_3:2590174-2590193 Msa0096870:CDS 30.0%
! TTTTCTCTATCTTTGGACAA+TGG + chr1_3:2590225-2590244 None:intergenic 30.0%
! CACAATCTTTTCTCTATCTT+TGG + chr1_3:2590232-2590251 None:intergenic 30.0%
TGTTTCAATCATTGAAGACA+AGG - chr1_3:2590607-2590626 Msa0096870:intron 30.0%
AAACATCAACAAGAGGAATA+TGG - chr1_3:2590667-2590686 Msa0096870:intron 30.0%
TATGGAACTTGAAGATCAAA+TGG - chr1_3:2590685-2590704 Msa0096870:intron 30.0%
GAAAGACTTGATGAAATGTA+AGG - chr1_3:2590919-2590938 Msa0096870:intron 30.0%
TACTTTCACAATCTTTACAG+AGG + chr1_3:2590997-2591016 None:intergenic 30.0%
AATTTAGAGGATTGACATAG+AGG + chr1_3:2591034-2591053 None:intergenic 30.0%
TGTCAATCCTCTAAATTCAT+CGG - chr1_3:2591037-2591056 Msa0096870:intron 30.0%
AAACTAGTTACAATGTGTCA+CGG + chr1_3:2591198-2591217 None:intergenic 30.0%
!! AAAGATTTGGTTTGTGGTTG+AGG + chr1_3:2588800-2588819 None:intergenic 35.0%
AACCACAAACCAAATCTTTC+AGG - chr1_3:2588801-2588820 Msa0096870:three_prime_UTR 35.0%
!! TACCTGAAAGATTTGGTTTG+TGG + chr1_3:2588806-2588825 None:intergenic 35.0%
ACTGATCTTGCAAATCAGTA+GGG + chr1_3:2589047-2589066 None:intergenic 35.0%
TGAAGAATTTGCATACATGG+AGG + chr1_3:2589090-2589109 None:intergenic 35.0%
!!! TTTTTTGGTAGCTACCCTTT+TGG - chr1_3:2589210-2589229 Msa0096870:three_prime_UTR 35.0%
! GTAGATATAAGCTACCCTTT+TGG - chr1_3:2589344-2589363 Msa0096870:three_prime_UTR 35.0%
GTCACAACACATGAAAATGT+AGG + chr1_3:2589524-2589543 None:intergenic 35.0%
TTTGCATTGGAATTTCGGTT+TGG - chr1_3:2589639-2589658 Msa0096870:three_prime_UTR 35.0%
!! TCTTTTAGTCTTTTCCTCTC+TGG + chr1_3:2589667-2589686 None:intergenic 35.0%
GAGGAAAAGACTAAAAGAAC+AGG - chr1_3:2589669-2589688 Msa0096870:three_prime_UTR 35.0%
AATTCATCATGATCGGTAAC+TGG - chr1_3:2589999-2590018 Msa0096870:exon 35.0%
! GTATGCTGATGATTTGTAAG+TGG - chr1_3:2590059-2590078 Msa0096870:CDS 35.0%
GGTTAGAATGCATCACAATA+CGG + chr1_3:2590430-2590449 None:intergenic 35.0%
CTCTTACGAGTACAATATAC+AGG + chr1_3:2590451-2590470 None:intergenic 35.0%
TCTAATAGGGTGAACAATTG+CGG - chr1_3:2590543-2590562 Msa0096870:intron 35.0%
! TTTCTTTCTCGCGAAGTTTT+TGG + chr1_3:2590637-2590656 None:intergenic 35.0%
AAAACTTCGCGAGAAAGAAA+CGG - chr1_3:2590637-2590656 Msa0096870:intron 35.0%
AGGTAGAAAACATCAACAAG+AGG - chr1_3:2590660-2590679 Msa0096870:intron 35.0%
ACTATGATAAGCAACAACAC+AGG + chr1_3:2591138-2591157 None:intergenic 35.0%
GACTGATCTTGCAAATCAGT+AGG + chr1_3:2589048-2589067 None:intergenic 40.0%
ATACATGGAGGAATGTAAGG+TGG + chr1_3:2589078-2589097 None:intergenic 40.0%
TGCATACATGGAGGAATGTA+AGG + chr1_3:2589081-2589100 None:intergenic 40.0%
!!! TCATGTAGTTGGTTTTGCTC+CGG - chr1_3:2589576-2589595 Msa0096870:three_prime_UTR 40.0%
!! ATGGTGGTGTTGATTTGCAT+TGG - chr1_3:2589626-2589645 Msa0096870:three_prime_UTR 40.0%
!! TCGGCTTTATTGTCTCTCAT+AGG - chr1_3:2589805-2589824 Msa0096870:three_prime_UTR 40.0%
!! CGGCTTTATTGTCTCTCATA+GGG - chr1_3:2589806-2589825 Msa0096870:three_prime_UTR 40.0%
!! GGCTTTATTGTCTCTCATAG+GGG - chr1_3:2589807-2589826 Msa0096870:three_prime_UTR 40.0%
TCTCAAGTTTAACCTCCAAC+AGG - chr1_3:2590775-2590794 Msa0096870:intron 40.0%
AAGGTAGGGACAGTAAAGAA+GGG - chr1_3:2590807-2590826 Msa0096870:intron 40.0%
! GATACAGCTTCTTGCTGTAA+TGG - chr1_3:2590851-2590870 Msa0096870:intron 40.0%
ATCCTCTAAATTCATCGGCA+TGG - chr1_3:2591042-2591061 Msa0096870:intron 40.0%
! TGTCACGGTACTTGTTACAA+TGG + chr1_3:2591183-2591202 None:intergenic 40.0%
AGAAGAGAGAAGTCTCTCGA+GGG + chr1_3:2588694-2588713 None:intergenic 45.0%
TTGAAGAAAAGCCATGGAGG+AGG + chr1_3:2588741-2588760 None:intergenic 45.0%
GTCTTGAAGAAAAGCCATGG+AGG + chr1_3:2588744-2588763 None:intergenic 45.0%
!! CTGGTCTTGAAGAAAAGCCA+TGG + chr1_3:2588747-2588766 None:intergenic 45.0%
!! TTGGTTTGTGGTTGAGGTTG+TGG + chr1_3:2588794-2588813 None:intergenic 45.0%
! GGGGGTTTAGTTCATGTAGT+TGG - chr1_3:2589565-2589584 Msa0096870:three_prime_UTR 45.0%
!!! CATGTAGTTGGTTTTGCTCC+GGG - chr1_3:2589577-2589596 Msa0096870:three_prime_UTR 45.0%
!! ATTTCGGTTTGGAACCAGAG+AGG - chr1_3:2589650-2589669 Msa0096870:three_prime_UTR 45.0%
ACAGCAGCAGGATTTAGAGA+TGG - chr1_3:2589849-2589868 Msa0096870:three_prime_UTR 45.0%
GGACAGATTTCTCAAACTGC+AGG - chr1_3:2589870-2589889 Msa0096870:three_prime_UTR 45.0%
!! CTTTCTCGCGAAGTTTTTGG+AGG + chr1_3:2590634-2590653 None:intergenic 45.0%
GAACAGCTAAGTGTAGAAGC+AGG - chr1_3:2590707-2590726 Msa0096870:intron 45.0%
TACCTTGGAGATATGCCTGT+TGG + chr1_3:2590793-2590812 None:intergenic 45.0%
AACAGGCATATCTCCAAGGT+AGG - chr1_3:2590792-2590811 Msa0096870:intron 45.0%
ACAGGCATATCTCCAAGGTA+GGG - chr1_3:2590793-2590812 Msa0096870:intron 45.0%
CTTCTTTACTGTCCCTACCT+TGG + chr1_3:2590808-2590827 None:intergenic 45.0%
CAAGGTAGGGACAGTAAAGA+AGG - chr1_3:2590806-2590825 Msa0096870:intron 45.0%
CTCCATGCCGATGAATTTAG+AGG + chr1_3:2591047-2591066 None:intergenic 45.0%
CTCTAAATTCATCGGCATGG+AGG - chr1_3:2591045-2591064 Msa0096870:intron 45.0%
CATGGAGGTCTACATGTAAC+TGG - chr1_3:2591060-2591079 Msa0096870:intron 45.0%
AGGGAATGTGAATGGCGATG+AGG + chr1_3:2588675-2588694 None:intergenic 50.0%
GTCTCTCGAGGGAATGTGAA+TGG + chr1_3:2588683-2588702 None:intergenic 50.0%
CAGAAGAGAGAAGTCTCTCG+AGG + chr1_3:2588695-2588714 None:intergenic 50.0%
AAGAAAAGCCATGGAGGAGG+AGG + chr1_3:2588738-2588757 None:intergenic 50.0%
GTTGTGGTTGTTGCTGGTAG+TGG + chr1_3:2588778-2588797 None:intergenic 50.0%
GTTGAGGTTGTGGTTGTTGC+TGG + chr1_3:2588784-2588803 None:intergenic 50.0%
ATTCCGGCTGATGGAAGTGA+TGG - chr1_3:2589607-2589626 Msa0096870:three_prime_UTR 50.0%
TGATCGTGAGTTACAGCAGC+AGG - chr1_3:2589837-2589856 Msa0096870:three_prime_UTR 50.0%
! CTCAAACTGCAGGTTAGCTC+AGG - chr1_3:2589880-2589899 Msa0096870:three_prime_UTR 50.0%
ACTTCGCGAGAAAGAAACGG+AGG - chr1_3:2590640-2590659 Msa0096870:intron 50.0%
TAAGTGTAGAAGCAGGCGCA+TGG - chr1_3:2590714-2590733 Msa0096870:intron 50.0%
CTTGGAGATATGCCTGTTGG+AGG + chr1_3:2590790-2590809 None:intergenic 50.0%
CTCCAACAGGCATATCTCCA+AGG - chr1_3:2590788-2590807 Msa0096870:intron 50.0%
AGGGACAGTAAAGAAGGGTG+TGG - chr1_3:2590812-2590831 Msa0096870:intron 50.0%
CAGAGTCAACGAAGTGACGA+TGG - chr1_3:2590946-2590965 Msa0096870:intron 50.0%
CAGAGGCAAAGATGCTTGCA+AGG + chr1_3:2590980-2590999 None:intergenic 50.0%
TTCTTCCTCCTCCTCCTCCA+TGG - chr1_3:2588727-2588746 Msa0096870:three_prime_UTR 55.0%
AAAAGCCATGGAGGAGGAGG+AGG + chr1_3:2588735-2588754 None:intergenic 55.0%
!! GCTGGTAGTGGCGTTGAAAC+TGG + chr1_3:2588766-2588785 None:intergenic 55.0%
AGGTCAGATGTCGCAGCAGA+TGG - chr1_3:2589011-2589030 Msa0096870:three_prime_UTR 55.0%
CTTCCATCAGCCGGAATGAC+AGG + chr1_3:2589604-2589623 None:intergenic 55.0%
CCACCATCACTTCCATCAGC+CGG + chr1_3:2589613-2589632 None:intergenic 55.0%
GCAACCTCGATCTGTGTCCA+CGG - chr1_3:2589735-2589754 Msa0096870:three_prime_UTR 55.0%
CAACCTCGATCTGTGTCCAC+GGG - chr1_3:2589736-2589755 Msa0096870:three_prime_UTR 55.0%
AACCTCGATCTGTGTCCACG+GGG - chr1_3:2589737-2589756 Msa0096870:three_prime_UTR 55.0%
AACACTCGCCTGGCTTCAAC+AGG - chr1_3:2589778-2589797 Msa0096870:three_prime_UTR 55.0%
CCTGGCTTCAACAGGAGACT+CGG - chr1_3:2589786-2589805 Msa0096870:three_prime_UTR 55.0%
TGCTCCGGGTCCTGTCATTC+CGG - chr1_3:2589591-2589610 Msa0096870:three_prime_UTR 60.0%
TCAGCCGGAATGACAGGACC+CGG + chr1_3:2589598-2589617 None:intergenic 60.0%
GGTCCTGTCATTCCGGCTGA+TGG - chr1_3:2589598-2589617 Msa0096870:three_prime_UTR 60.0%
CCGGCTGATGGAAGTGATGG+TGG - chr1_3:2589610-2589629 Msa0096870:three_prime_UTR 60.0%
AACCCCGTGGACACAGATCG+AGG + chr1_3:2589742-2589761 None:intergenic 60.0%
CGATCTGTGTCCACGGGGTT+AGG - chr1_3:2589742-2589761 Msa0096870:three_prime_UTR 60.0%
GAGCGACAATCCTAACCCCG+TGG + chr1_3:2589755-2589774 None:intergenic 60.0%
GTCGCTCGATAACACTCGCC+TGG - chr1_3:2589768-2589787 Msa0096870:three_prime_UTR 60.0%
CCGAGTCTCCTGTTGAAGCC+AGG + chr1_3:2589789-2589808 None:intergenic 60.0%
! AGAAGGGTGTGGTGACAGCG+AGG - chr1_3:2590823-2590842 Msa0096870:intron 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 2588632 2591294 2588632 ID=Msa0096870;Name=Msa0096870
chr1_3 mRNA 2588632 2591294 2588632 ID=Msa0096870-mRNA-1;Parent=Msa0096870;Name=Msa0096870-mRNA-1;_AED=0.04;_eAED=0.04;_QI=114|0|0.66|0.66|1|1|3|1373|143
chr1_3 exon 2588632 2590351 2588632 ID=Msa0096870-mRNA-1:exon:703;Parent=Msa0096870-mRNA-1
chr1_3 exon 2590710 2590717 2590710 ID=Msa0096870-mRNA-1:exon:702;Parent=Msa0096870-mRNA-1
chr1_3 exon 2591104 2591294 2591104 ID=Msa0096870-mRNA-1:exon:701;Parent=Msa0096870-mRNA-1
chr1_3 five_prime_UTR 2591181 2591294 2591181 ID=Msa0096870-mRNA-1:five_prime_utr;Parent=Msa0096870-mRNA-1
chr1_3 CDS 2591104 2591180 2591104 ID=Msa0096870-mRNA-1:cds;Parent=Msa0096870-mRNA-1
chr1_3 CDS 2590710 2590717 2590710 ID=Msa0096870-mRNA-1:cds;Parent=Msa0096870-mRNA-1
chr1_3 CDS 2590005 2590351 2590005 ID=Msa0096870-mRNA-1:cds;Parent=Msa0096870-mRNA-1
chr1_3 three_prime_UTR 2588632 2590004 2588632 ID=Msa0096870-mRNA-1:three_prime_utr;Parent=Msa0096870-mRNA-1
Gene Sequence

>Msa0096870

ATGGCTTTTCTTCAAGACCAGTTTCAACGCCACTACCAGCAACAACCACAACCTCAACCACAAACCAAATCTTTCAGGTTTTTTGTTCATGTAGTTGGTTTTGCTCCGGGTCCTGTCATTCCGGCTGATGGAAGTGATGGTGGTGTTGATTTGCATTGGAATTTCGGTTTGGAACCAGAGAGGAAAAGACTAAAAGAACAGGATTTTTTGGAGAATAATTCTCAGATATCTTCTGTGGATTTCTTGCAACCTCGATCTGTGTCCACGGGGTTAGGATTGTCGCTCGATAACACTCGCCTGGCTTCAACAGGAGACTCGGCTTTATTGTCTCTCATAGGGGATGACATTGATCGTGAGTTACAGCAGCAGGATTTAGAGATGGACAGATTTCTCAAACTGCAGGTTAGCTCAGGTTTTCTATGTCTAGTTTAG
Protein sequence

>Msa0096870

MAFLQDQFQRHYQQQPQPQPQTKSFRFFVHVVGFAPGPVIPADGSDGGVDLHWNFGLEPERKRLKEQDFLENNSQISSVDFLQPRSVSTGLGLSLDNTRLASTGDSALLSLIGDDIDRELQQQDLEMDRFLKLQVSSGFLCLV*