AlfalfaGEDB | Msa0097660

M. sativa cultivar zhongmu-4 / Msa0097660

KEGG: -
GO: GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009739 GO:0009743 GO:0009756 GO:0009791 GO:0009845 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010029 GO:0010033 GO:0010035 GO:0010154 GO:0010182 GO:0010187 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0033993 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071310 GO:0071322 GO:0080090 GO:0090351 GO:0097305 GO:0140110 GO:1900140 GO:1901698 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000026 GO:2000112 GO:2001141
PFAM: PF00170.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0097660	KEH40004.1	97.241	145	4	0	1	145	1	145	1.10e-97	287

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0097660	sp\|Q9SI15\|BZIP2_ARATH	43.750	80	45	0	45	124	16	95	5.76e-13	65.9

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0097660	A0A072VDB1	97.241	145	4	0	1	145	1	145	5.25e-98	287

TFs/TRs:

Gene ID	Type	Classification
Msa0097660	TF	bZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0052840	Msa0097660	0.898876	3.449878e-77	-8.615850e-47
Msa0097660	Msa0097880	0.921762	2.359963e-88	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0097660	MtrunA17_Chr1g0152241	97.241	145	4	0	1	145	1	145	1.01e-101	287
Msa0097660	MtrunA17_Chr3g0135061	58.333	144	56	2	1	144	1	140	6.13e-50	156
Msa0097660	MtrunA17_Chr4g0054291	47.154	123	47	3	22	128	23	143	6.61e-20	80.9
Msa0097660	MtrunA17_Chr5g0400901	45.082	122	51	3	31	139	22	140	4.62e-18	75.9
Msa0097660	MtrunA17_Chr8g0378901	41.584	101	53	2	45	143	18	114	8.68e-15	67.4
Msa0097660	MtrunA17_Chr1g0194681	37.273	110	62	2	34	143	4	106	1.28e-13	63.9
Msa0097660	MtrunA17_Chr1g0155551	41.111	90	51	1	35	124	7	94	1.85e-12	61.2
Msa0097660	MtrunA17_Chr3g0144941	43.902	82	44	1	43	124	15	94	2.03e-12	60.8
Msa0097660	MtrunA17_Chr4g0035414	43.750	80	43	1	45	124	17	94	2.43e-12	60.8
Msa0097660	MtrunA17_Chr7g0225661	44.156	77	43	0	59	135	83	159	1.18e-11	59.7
Msa0097660	MtrunA17_Chr8g0341311	42.857	77	44	0	59	135	73	149	1.76e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0097660	AT3G49760.1	38.849	139	77	2	9	139	13	151	1.30e-20	82.8
Msa0097660	AT2G22850.1	45.783	83	45	0	57	139	126	208	2.32e-15	70.5
Msa0097660	AT2G22850.2	45.783	83	45	0	57	139	126	208	2.32e-15	70.5
Msa0097660	AT2G18160.1	43.750	80	45	0	45	124	16	95	5.86e-14	65.9
Msa0097660	AT1G75390.1	41.667	84	49	0	45	128	26	109	3.30e-13	63.9
Msa0097660	AT4G37730.1	51.471	68	33	0	57	124	194	261	3.94e-13	65.5
Msa0097660	AT1G75390.2	41.463	82	48	0	43	124	24	105	1.01e-12	61.2
Msa0097660	AT1G59530.1	39.344	122	68	2	18	139	14	129	1.02e-11	59.3
Msa0097660	AT5G15830.1	38.835	103	59	2	26	128	44	142	1.02e-11	60.1

Find 45 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCCCTCATGGCAACACTTT+TGG	0.232653	1_3:+3485072	Msa0097660:CDS
CGATTTGAACCGGTTGAGAA+TGG	0.382249	1_3:+3484958	Msa0097660:CDS
TTGTGAACCGGACCCTGGAC+TGG	0.414459	1_3:-3484816	None:intergenic
ACCGGTTGAGAATGGAAAAC+CGG	0.420192	1_3:+3484966	Msa0097660:CDS
GGTCCGGCTCACGTGTGTTC+CGG	0.430584	1_3:+3484721	Msa0097660:CDS
TAAGGAGAATGCAATCAAAC+CGG	0.448119	1_3:+3484879	Msa0097660:CDS
ACCTCACGAACGATTTGAAC+CGG	0.459693	1_3:+3484948	Msa0097660:CDS
ATTGCATGGTTAACCCTAGC+TGG	0.474346	1_3:-3485000	None:intergenic
GGACCGGATACTCCTCTTCA+TGG	0.477097	1_3:-3484703	None:intergenic
ACGACTTGATCCTTGTGAAC+CGG	0.478619	1_3:-3484828	None:intergenic
GTGAACATGGTTTCATGAAC+CGG	0.479700	1_3:-3484740	None:intergenic
CCTGGACTGGATGGATCAAC+CGG	0.484260	1_3:-3484803	None:intergenic
CCGGTTGATCCATCCAGTCC+AGG	0.489171	1_3:+3484803	Msa0097660:CDS
GATCCTTGTGAACCGGACCC+TGG	0.490890	1_3:-3484821	None:intergenic
GATCAACCGGTTGGTTAATG+AGG	0.507162	1_3:-3484790	None:intergenic
GAACCGGAACACACGTGAGC+CGG	0.519769	1_3:-3484724	None:intergenic
GATCCATCCAGTCCAGGGTC+CGG	0.524687	1_3:+3484809	Msa0097660:CDS
CTTCCATGAAGAGGAGTATC+CGG	0.526400	1_3:+3484700	Msa0097660:CDS
GGAACACACGTGAGCCGGAC+CGG	0.528631	1_3:-3484719	None:intergenic
GTTCACACAAGGCGAAATAG+AGG	0.540753	1_3:+3484757	Msa0097660:CDS
ATCCAAAAGTGTTGCCATGA+GGG	0.543780	1_3:-3485074	None:intergenic
TATTTCGCCTTGTGTGAACA+TGG	0.548387	1_3:-3484753	None:intergenic
CCGGTTGAGAATGGAAAACC+GGG	0.557045	1_3:+3484967	Msa0097660:CDS
AATCAAACCGGGAGTCGGCT+AGG	0.560261	1_3:+3484891	Msa0097660:CDS
ATCAGAATCAATGTCCCTCA+TGG	0.566320	1_3:+3485060	Msa0097660:CDS
AGCAATGTCCTTCCATGAAG+AGG	0.571717	1_3:+3484691	None:intergenic
GAACCGGACCCTGGACTGGA+TGG	0.582397	1_3:-3484812	None:intergenic
ATGAAGAGGAGTATCCGGTC+CGG	0.588642	1_3:+3484705	Msa0097660:CDS
AGTCCAGGGTCCGGTTCACA+AGG	0.591544	1_3:+3484818	Msa0097660:CDS
ACCGGTTCAAATCGTTCGTG+AGG	0.594738	1_3:-3484949	None:intergenic
CGGATACTCCTCTTCATGGA+AGG	0.596073	1_3:-3484699	None:intergenic
CGGTTGATCCATCCAGTCCA+GGG	0.598027	1_3:+3484804	Msa0097660:CDS
GACTGGATGGATCAACCGGT+TGG	0.601414	1_3:-3484799	None:intergenic
GTGACCGCCTAGCCGACTCC+CGG	0.602249	1_3:-3484898	None:intergenic
TAATATGAGATTGTATTGCA+TGG	0.609971	1_3:-3485014	None:intergenic
ATCAACCGGTTGGTTAATGA+GGG	0.611428	1_3:-3484789	None:intergenic
AATGCAATCAAACCGGGAGT+CGG	0.621410	1_3:+3484886	Msa0097660:CDS
ATACAATCTCATATTATCAA+CGG	0.623568	1_3:+3485021	Msa0097660:CDS
AAGGAGAATGCAATCAAACC+GGG	0.631263	1_3:+3484880	Msa0097660:CDS
CATGAAACCATGTTCACACA+AGG	0.636957	1_3:+3484746	Msa0097660:CDS
GATCCAAAAGTGTTGCCATG+AGG	0.664933	1_3:-3485075	None:intergenic
TTCACAAGGATCAAGTCGTG+CGG	0.675020	1_3:+3484832	Msa0097660:CDS
AGTCGGCTAGGCGGTCACGT+TGG	0.676204	1_3:+3484903	Msa0097660:CDS
GGTCACGTTGGAGAAAGAAG+CGG	0.707696	1_3:+3484915	Msa0097660:CDS
CAAACCGGGAGTCGGCTAGG+CGG	0.728540	1_3:+3484894	Msa0097660:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATACAATCTCATATTATCAA+CGG	+	chr1_3:3485021-3485040	Msa0097660:CDS	20.0%
!	TAATATGAGATTGTATTGCA+TGG	-	chr1_3:3485017-3485036	None:intergenic	25.0%
!!!	TTCTCCTTATTTTTCTCTCA+CGG	-	chr1_3:3484868-3484887	None:intergenic	30.0%
!	CAACACTTTTGGATCTATAT+CGG	+	chr1_3:3485083-3485102	Msa0097660:CDS	30.0%
!	ATTTTCCCTCATTAACCAAC+CGG	+	chr1_3:3484784-3484803	Msa0097660:CDS	35.0%
!!!	TCTCCTTATTTTTCTCTCAC+GGG	-	chr1_3:3484867-3484886	None:intergenic	35.0%
	TAAGGAGAATGCAATCAAAC+CGG	+	chr1_3:3484879-3484898	Msa0097660:CDS	35.0%
	GTGAACATGGTTTCATGAAC+CGG	-	chr1_3:3484743-3484762	None:intergenic	40.0%
	CATGAAACCATGTTCACACA+AGG	+	chr1_3:3484746-3484765	Msa0097660:CDS	40.0%
	TATTTCGCCTTGTGTGAACA+TGG	-	chr1_3:3484756-3484775	None:intergenic	40.0%
!	ATCAACCGGTTGGTTAATGA+GGG	-	chr1_3:3484792-3484811	None:intergenic	40.0%
	AAGGAGAATGCAATCAAACC+GGG	+	chr1_3:3484880-3484899	Msa0097660:CDS	40.0%
!!!	CTAGCTGGTTTTTTAGTTCC+CGG	-	chr1_3:3484988-3485007	None:intergenic	40.0%
	GGGAACTAAAAAACCAGCTA+GGG	+	chr1_3:3484987-3485006	Msa0097660:CDS	40.0%
	ATCAGAATCAATGTCCCTCA+TGG	+	chr1_3:3485060-3485079	Msa0097660:CDS	40.0%
!!	ATCCAAAAGTGTTGCCATGA+GGG	-	chr1_3:3485077-3485096	None:intergenic	40.0%
!!	CTTCCATGAAGAGGAGTATC+CGG	+	chr1_3:3484700-3484719	Msa0097660:CDS	45.0%
	GTTCACACAAGGCGAAATAG+AGG	+	chr1_3:3484757-3484776	Msa0097660:CDS	45.0%
!	GATCAACCGGTTGGTTAATG+AGG	-	chr1_3:3484793-3484812	None:intergenic	45.0%
	ACGACTTGATCCTTGTGAAC+CGG	-	chr1_3:3484831-3484850	None:intergenic	45.0%
	TTCACAAGGATCAAGTCGTG+CGG	+	chr1_3:3484832-3484851	Msa0097660:CDS	45.0%
	CGACCCGTGAGAGAAAAATA+AGG	+	chr1_3:3484861-3484880	Msa0097660:CDS	45.0%
	AATGCAATCAAACCGGGAGT+CGG	+	chr1_3:3484886-3484905	Msa0097660:CDS	45.0%
	ACCTCACGAACGATTTGAAC+CGG	+	chr1_3:3484948-3484967	Msa0097660:CDS	45.0%
	CGATTTGAACCGGTTGAGAA+TGG	+	chr1_3:3484958-3484977	Msa0097660:CDS	45.0%
	ACCGGTTGAGAATGGAAAAC+CGG	+	chr1_3:3484966-3484985	Msa0097660:CDS	45.0%
	CGGGAACTAAAAAACCAGCT+AGG	+	chr1_3:3484986-3485005	Msa0097660:CDS	45.0%
	ATTGCATGGTTAACCCTAGC+TGG	-	chr1_3:3485003-3485022	None:intergenic	45.0%
!!	GATCCAAAAGTGTTGCCATG+AGG	-	chr1_3:3485078-3485097	None:intergenic	45.0%
	CGGATACTCCTCTTCATGGA+AGG	-	chr1_3:3484702-3484721	None:intergenic	50.0%
!!	ATGAAGAGGAGTATCCGGTC+CGG	+	chr1_3:3484705-3484724	Msa0097660:CDS	50.0%
	GGTCACGTTGGAGAAAGAAG+CGG	+	chr1_3:3484915-3484934	Msa0097660:CDS	50.0%
!	ACCGGTTCAAATCGTTCGTG+AGG	-	chr1_3:3484952-3484971	None:intergenic	50.0%
!	CCCGGTTTTCCATTCTCAAC+CGG	-	chr1_3:3484970-3484989	None:intergenic	50.0%
	CCGGTTGAGAATGGAAAACC+GGG	+	chr1_3:3484967-3484986	Msa0097660:CDS	50.0%
!	GTCCCTCATGGCAACACTTT+TGG	+	chr1_3:3485072-3485091	Msa0097660:CDS	50.0%
	GGACCGGATACTCCTCTTCA+TGG	-	chr1_3:3484706-3484725	None:intergenic	55.0%
	GACTGGATGGATCAACCGGT+TGG	-	chr1_3:3484802-3484821	None:intergenic	55.0%
!	CCTGGACTGGATGGATCAAC+CGG	-	chr1_3:3484806-3484825	None:intergenic	55.0%
	CGGTTGATCCATCCAGTCCA+GGG	+	chr1_3:3484804-3484823	Msa0097660:CDS	55.0%
	AATCAAACCGGGAGTCGGCT+AGG	+	chr1_3:3484891-3484910	Msa0097660:CDS	55.0%
	GAACCGGAACACACGTGAGC+CGG	-	chr1_3:3484727-3484746	None:intergenic	60.0%
	CCGGTTGATCCATCCAGTCC+AGG	+	chr1_3:3484803-3484822	Msa0097660:CDS	60.0%
	GATCCATCCAGTCCAGGGTC+CGG	+	chr1_3:3484809-3484828	Msa0097660:CDS	60.0%
	TTGTGAACCGGACCCTGGAC+TGG	-	chr1_3:3484819-3484838	None:intergenic	60.0%
	AGTCCAGGGTCCGGTTCACA+AGG	+	chr1_3:3484818-3484837	Msa0097660:CDS	60.0%
	GATCCTTGTGAACCGGACCC+TGG	-	chr1_3:3484824-3484843	None:intergenic	60.0%
	GGAACACACGTGAGCCGGAC+CGG	-	chr1_3:3484722-3484741	None:intergenic	65.0%
	GGTCCGGCTCACGTGTGTTC+CGG	+	chr1_3:3484721-3484740	Msa0097660:CDS	65.0%
!	GAACCGGACCCTGGACTGGA+TGG	-	chr1_3:3484815-3484834	None:intergenic	65.0%
	CAAACCGGGAGTCGGCTAGG+CGG	+	chr1_3:3484894-3484913	Msa0097660:CDS	65.0%
	AGTCGGCTAGGCGGTCACGT+TGG	+	chr1_3:3484903-3484922	Msa0097660:CDS	65.0%
!	GTGACCGCCTAGCCGACTCC+CGG	-	chr1_3:3484901-3484920	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	3484695	3485132	3484695	ID=Msa0097660;Name=Msa0097660
chr1_3	mRNA	3484695	3485132	3484695	ID=Msa0097660-mRNA-1;Parent=Msa0097660;Name=Msa0097660-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|145
chr1_3	exon	3484695	3485132	3484695	ID=Msa0097660-mRNA-1:exon:1030;Parent=Msa0097660-mRNA-1
chr1_3	CDS	3484695	3485132	3484695	ID=Msa0097660-mRNA-1:cds;Parent=Msa0097660-mRNA-1

Gene Sequence

>Msa0097660

ATGTCCTTCCATGAAGAGGAGTATCCGGTCCGGCTCACGTGTGTTCCGGTTCATGAAACCATGTTCACACAAGGCGAAATAGAGGAGTTATTTTCCCTCATTAACCAACCGGTTGATCCATCCAGTCCAGGGTCCGGTTCACAAGGATCAAGTCGTGCGGTTTACTCGACCCGTGAGAGAAAAATAAGGAGAATGCAATCAAACCGGGAGTCGGCTAGGCGGTCACGTTGGAGAAAGAAGCGGCACGTAGAGAACCTCACGAACGATTTGAACCGGTTGAGAATGGAAAACCGGGAACTAAAAAACCAGCTAGGGTTAACCATGCAATACAATCTCATATTATCAACGGAGAATGAACGTCTAAGATCAGAATCAATGTCCCTCATGGCAACACTTTTGGATCTATATCGGACTTTAGAGACAATAATTTCACAATAG

Protein sequence

>Msa0097660

MSFHEEEYPVRLTCVPVHETMFTQGEIEELFSLINQPVDPSSPGSGSQGSSRAVYSTRERKIRRMQSNRESARRSRWRKKRHVENLTNDLNRLRMENRELKNQLGLTMQYNLILSTENERLRSESMSLMATLLDLYRTLETIISQ*