AlfalfaGEDB | Msa0097880

M. sativa cultivar zhongmu-4 / Msa0097880

KEGG: -
GO: GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009739 GO:0009743 GO:0009756 GO:0009791 GO:0009845 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010029 GO:0010033 GO:0010035 GO:0010154 GO:0010182 GO:0010187 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0033993 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071310 GO:0071322 GO:0080090 GO:0090351 GO:0097305 GO:0140110 GO:1900140 GO:1901698 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000026 GO:2000112 GO:2001141
PFAM: PF00170.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0097880	KEH40004.1	97.241	145	4	0	1	145	1	145	1.10e-97	287

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0097880	sp\|Q9SI15\|BZIP2_ARATH	43.750	80	45	0	45	124	16	95	5.76e-13	65.9

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0097880	A0A072VDB1	97.241	145	4	0	1	145	1	145	5.25e-98	287

TFs/TRs:

Gene ID	Type	Classification
Msa0097880	TF	bZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0052840	Msa0097880	0.964445	2.458322e-123	-8.615850e-47
Msa0097660	Msa0097880	0.921762	2.359963e-88	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0097880	MtrunA17_Chr1g0152241	97.241	145	4	0	1	145	1	145	1.01e-101	287
Msa0097880	MtrunA17_Chr3g0135061	58.333	144	56	2	1	144	1	140	6.13e-50	156
Msa0097880	MtrunA17_Chr4g0054291	47.154	123	47	3	22	128	23	143	6.61e-20	80.9
Msa0097880	MtrunA17_Chr5g0400901	45.082	122	51	3	31	139	22	140	4.62e-18	75.9
Msa0097880	MtrunA17_Chr8g0378901	41.584	101	53	2	45	143	18	114	8.68e-15	67.4
Msa0097880	MtrunA17_Chr1g0194681	37.273	110	62	2	34	143	4	106	1.28e-13	63.9
Msa0097880	MtrunA17_Chr1g0155551	41.111	90	51	1	35	124	7	94	1.85e-12	61.2
Msa0097880	MtrunA17_Chr3g0144941	43.902	82	44	1	43	124	15	94	2.03e-12	60.8
Msa0097880	MtrunA17_Chr4g0035414	43.750	80	43	1	45	124	17	94	2.43e-12	60.8
Msa0097880	MtrunA17_Chr7g0225661	44.156	77	43	0	59	135	83	159	1.18e-11	59.7
Msa0097880	MtrunA17_Chr8g0341311	42.857	77	44	0	59	135	73	149	1.76e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0097880	AT3G49760.1	38.849	139	77	2	9	139	13	151	1.30e-20	82.8
Msa0097880	AT2G22850.1	45.783	83	45	0	57	139	126	208	2.32e-15	70.5
Msa0097880	AT2G22850.2	45.783	83	45	0	57	139	126	208	2.32e-15	70.5
Msa0097880	AT2G18160.1	43.750	80	45	0	45	124	16	95	5.86e-14	65.9
Msa0097880	AT1G75390.1	41.667	84	49	0	45	128	26	109	3.30e-13	63.9
Msa0097880	AT4G37730.1	51.471	68	33	0	57	124	194	261	3.94e-13	65.5
Msa0097880	AT1G75390.2	41.463	82	48	0	43	124	24	105	1.01e-12	61.2
Msa0097880	AT1G59530.1	39.344	122	68	2	18	139	14	129	1.02e-11	59.3
Msa0097880	AT5G15830.1	38.835	103	59	2	26	128	44	142	1.02e-11	60.1

Find 45 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCCCTCATGGCAACACTTT+TGG	0.232653	1_3:+3714814	Msa0097880:CDS
CGATTTGAACCGGTTGAGAA+TGG	0.382249	1_3:+3714700	Msa0097880:CDS
TTGTGAACCGGACCCTGGAC+TGG	0.414459	1_3:-3714558	None:intergenic
ACCGGTTGAGAATGGAAAAC+CGG	0.420192	1_3:+3714708	Msa0097880:CDS
GGTCCGGCTCACGTGTGTTC+CGG	0.430584	1_3:+3714463	Msa0097880:CDS
TAAGGAGAATGCAATCAAAC+CGG	0.448119	1_3:+3714621	Msa0097880:CDS
ACCTCACGAACGATTTGAAC+CGG	0.459693	1_3:+3714690	Msa0097880:CDS
ATTGCATGGTTAACCCTAGC+TGG	0.474346	1_3:-3714742	None:intergenic
GGACCGGATACTCCTCTTCA+TGG	0.477097	1_3:-3714445	None:intergenic
ACGACTTGATCCTTGTGAAC+CGG	0.478619	1_3:-3714570	None:intergenic
GTGAACATGGTTTCATGAAC+CGG	0.479700	1_3:-3714482	None:intergenic
CCTGGACTGGATGGATCAAC+CGG	0.484260	1_3:-3714545	None:intergenic
CCGGTTGATCCATCCAGTCC+AGG	0.489171	1_3:+3714545	Msa0097880:CDS
GATCCTTGTGAACCGGACCC+TGG	0.490890	1_3:-3714563	None:intergenic
GATCAACCGGTTGGTTAATG+AGG	0.507162	1_3:-3714532	None:intergenic
GAACCGGAACACACGTGAGC+CGG	0.519769	1_3:-3714466	None:intergenic
GATCCATCCAGTCCAGGGTC+CGG	0.524687	1_3:+3714551	Msa0097880:CDS
CTTCCATGAAGAGGAGTATC+CGG	0.526400	1_3:+3714442	Msa0097880:CDS
GGAACACACGTGAGCCGGAC+CGG	0.528631	1_3:-3714461	None:intergenic
GTTCACACAAGGCGAAATAG+AGG	0.540753	1_3:+3714499	Msa0097880:CDS
ATCCAAAAGTGTTGCCATGA+GGG	0.543780	1_3:-3714816	None:intergenic
TATTTCGCCTTGTGTGAACA+TGG	0.548387	1_3:-3714495	None:intergenic
CCGGTTGAGAATGGAAAACC+GGG	0.557045	1_3:+3714709	Msa0097880:CDS
AATCAAACCGGGAGTCGGCT+AGG	0.560261	1_3:+3714633	Msa0097880:CDS
ATCAGAATCAATGTCCCTCA+TGG	0.566320	1_3:+3714802	Msa0097880:CDS
AGCAATGTCCTTCCATGAAG+AGG	0.576455	1_3:+3714433	None:intergenic
GAACCGGACCCTGGACTGGA+TGG	0.582397	1_3:-3714554	None:intergenic
ATGAAGAGGAGTATCCGGTC+CGG	0.588642	1_3:+3714447	Msa0097880:CDS
AGTCCAGGGTCCGGTTCACA+AGG	0.591544	1_3:+3714560	Msa0097880:CDS
ACCGGTTCAAATCGTTCGTG+AGG	0.594738	1_3:-3714691	None:intergenic
CGGATACTCCTCTTCATGGA+AGG	0.596073	1_3:-3714441	None:intergenic
CGGTTGATCCATCCAGTCCA+GGG	0.598027	1_3:+3714546	Msa0097880:CDS
GACTGGATGGATCAACCGGT+TGG	0.601414	1_3:-3714541	None:intergenic
GTGACCGCCTAGCCGACTCC+CGG	0.602249	1_3:-3714640	None:intergenic
TAATATGAGATTGTATTGCA+TGG	0.609971	1_3:-3714756	None:intergenic
ATCAACCGGTTGGTTAATGA+GGG	0.611428	1_3:-3714531	None:intergenic
AATGCAATCAAACCGGGAGT+CGG	0.621410	1_3:+3714628	Msa0097880:CDS
ATACAATCTCATATTATCAA+CGG	0.623568	1_3:+3714763	Msa0097880:CDS
AAGGAGAATGCAATCAAACC+GGG	0.631263	1_3:+3714622	Msa0097880:CDS
CATGAAACCATGTTCACACA+AGG	0.636957	1_3:+3714488	Msa0097880:CDS
GATCCAAAAGTGTTGCCATG+AGG	0.664933	1_3:-3714817	None:intergenic
TTCACAAGGATCAAGTCGTG+CGG	0.675020	1_3:+3714574	Msa0097880:CDS
AGTCGGCTAGGCGGTCACGT+TGG	0.676204	1_3:+3714645	Msa0097880:CDS
GGTCACGTTGGAGAAAGAAG+CGG	0.707696	1_3:+3714657	Msa0097880:CDS
CAAACCGGGAGTCGGCTAGG+CGG	0.728540	1_3:+3714636	Msa0097880:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATACAATCTCATATTATCAA+CGG	+	chr1_3:3714763-3714782	Msa0097880:CDS	20.0%
!	TAATATGAGATTGTATTGCA+TGG	-	chr1_3:3714759-3714778	None:intergenic	25.0%
!!!	TTCTCCTTATTTTTCTCTCA+CGG	-	chr1_3:3714610-3714629	None:intergenic	30.0%
!	CAACACTTTTGGATCTATAT+CGG	+	chr1_3:3714825-3714844	Msa0097880:CDS	30.0%
!	ATTTTCCCTCATTAACCAAC+CGG	+	chr1_3:3714526-3714545	Msa0097880:CDS	35.0%
!!!	TCTCCTTATTTTTCTCTCAC+GGG	-	chr1_3:3714609-3714628	None:intergenic	35.0%
	TAAGGAGAATGCAATCAAAC+CGG	+	chr1_3:3714621-3714640	Msa0097880:CDS	35.0%
	GTGAACATGGTTTCATGAAC+CGG	-	chr1_3:3714485-3714504	None:intergenic	40.0%
	CATGAAACCATGTTCACACA+AGG	+	chr1_3:3714488-3714507	Msa0097880:CDS	40.0%
	TATTTCGCCTTGTGTGAACA+TGG	-	chr1_3:3714498-3714517	None:intergenic	40.0%
!	ATCAACCGGTTGGTTAATGA+GGG	-	chr1_3:3714534-3714553	None:intergenic	40.0%
	AAGGAGAATGCAATCAAACC+GGG	+	chr1_3:3714622-3714641	Msa0097880:CDS	40.0%
!!!	CTAGCTGGTTTTTTAGTTCC+CGG	-	chr1_3:3714730-3714749	None:intergenic	40.0%
	GGGAACTAAAAAACCAGCTA+GGG	+	chr1_3:3714729-3714748	Msa0097880:CDS	40.0%
	ATCAGAATCAATGTCCCTCA+TGG	+	chr1_3:3714802-3714821	Msa0097880:CDS	40.0%
!!	ATCCAAAAGTGTTGCCATGA+GGG	-	chr1_3:3714819-3714838	None:intergenic	40.0%
!!	CTTCCATGAAGAGGAGTATC+CGG	+	chr1_3:3714442-3714461	Msa0097880:CDS	45.0%
	GTTCACACAAGGCGAAATAG+AGG	+	chr1_3:3714499-3714518	Msa0097880:CDS	45.0%
!	GATCAACCGGTTGGTTAATG+AGG	-	chr1_3:3714535-3714554	None:intergenic	45.0%
	ACGACTTGATCCTTGTGAAC+CGG	-	chr1_3:3714573-3714592	None:intergenic	45.0%
	TTCACAAGGATCAAGTCGTG+CGG	+	chr1_3:3714574-3714593	Msa0097880:CDS	45.0%
	CGACCCGTGAGAGAAAAATA+AGG	+	chr1_3:3714603-3714622	Msa0097880:CDS	45.0%
	AATGCAATCAAACCGGGAGT+CGG	+	chr1_3:3714628-3714647	Msa0097880:CDS	45.0%
	ACCTCACGAACGATTTGAAC+CGG	+	chr1_3:3714690-3714709	Msa0097880:CDS	45.0%
	CGATTTGAACCGGTTGAGAA+TGG	+	chr1_3:3714700-3714719	Msa0097880:CDS	45.0%
	ACCGGTTGAGAATGGAAAAC+CGG	+	chr1_3:3714708-3714727	Msa0097880:CDS	45.0%
	CGGGAACTAAAAAACCAGCT+AGG	+	chr1_3:3714728-3714747	Msa0097880:CDS	45.0%
	ATTGCATGGTTAACCCTAGC+TGG	-	chr1_3:3714745-3714764	None:intergenic	45.0%
!!	GATCCAAAAGTGTTGCCATG+AGG	-	chr1_3:3714820-3714839	None:intergenic	45.0%
	CGGATACTCCTCTTCATGGA+AGG	-	chr1_3:3714444-3714463	None:intergenic	50.0%
!!	ATGAAGAGGAGTATCCGGTC+CGG	+	chr1_3:3714447-3714466	Msa0097880:CDS	50.0%
	GGTCACGTTGGAGAAAGAAG+CGG	+	chr1_3:3714657-3714676	Msa0097880:CDS	50.0%
!	ACCGGTTCAAATCGTTCGTG+AGG	-	chr1_3:3714694-3714713	None:intergenic	50.0%
!	CCCGGTTTTCCATTCTCAAC+CGG	-	chr1_3:3714712-3714731	None:intergenic	50.0%
	CCGGTTGAGAATGGAAAACC+GGG	+	chr1_3:3714709-3714728	Msa0097880:CDS	50.0%
!	GTCCCTCATGGCAACACTTT+TGG	+	chr1_3:3714814-3714833	Msa0097880:CDS	50.0%
	GGACCGGATACTCCTCTTCA+TGG	-	chr1_3:3714448-3714467	None:intergenic	55.0%
	GACTGGATGGATCAACCGGT+TGG	-	chr1_3:3714544-3714563	None:intergenic	55.0%
!	CCTGGACTGGATGGATCAAC+CGG	-	chr1_3:3714548-3714567	None:intergenic	55.0%
	CGGTTGATCCATCCAGTCCA+GGG	+	chr1_3:3714546-3714565	Msa0097880:CDS	55.0%
	AATCAAACCGGGAGTCGGCT+AGG	+	chr1_3:3714633-3714652	Msa0097880:CDS	55.0%
	GAACCGGAACACACGTGAGC+CGG	-	chr1_3:3714469-3714488	None:intergenic	60.0%
	CCGGTTGATCCATCCAGTCC+AGG	+	chr1_3:3714545-3714564	Msa0097880:CDS	60.0%
	GATCCATCCAGTCCAGGGTC+CGG	+	chr1_3:3714551-3714570	Msa0097880:CDS	60.0%
	TTGTGAACCGGACCCTGGAC+TGG	-	chr1_3:3714561-3714580	None:intergenic	60.0%
	AGTCCAGGGTCCGGTTCACA+AGG	+	chr1_3:3714560-3714579	Msa0097880:CDS	60.0%
	GATCCTTGTGAACCGGACCC+TGG	-	chr1_3:3714566-3714585	None:intergenic	60.0%
	GGAACACACGTGAGCCGGAC+CGG	-	chr1_3:3714464-3714483	None:intergenic	65.0%
	GGTCCGGCTCACGTGTGTTC+CGG	+	chr1_3:3714463-3714482	Msa0097880:CDS	65.0%
!	GAACCGGACCCTGGACTGGA+TGG	-	chr1_3:3714557-3714576	None:intergenic	65.0%
	CAAACCGGGAGTCGGCTAGG+CGG	+	chr1_3:3714636-3714655	Msa0097880:CDS	65.0%
	AGTCGGCTAGGCGGTCACGT+TGG	+	chr1_3:3714645-3714664	Msa0097880:CDS	65.0%
!	GTGACCGCCTAGCCGACTCC+CGG	-	chr1_3:3714643-3714662	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	3714437	3714874	3714437	ID=Msa0097880;Name=Msa0097880
chr1_3	mRNA	3714437	3714874	3714437	ID=Msa0097880-mRNA-1;Parent=Msa0097880;Name=Msa0097880-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|145
chr1_3	exon	3714437	3714874	3714437	ID=Msa0097880-mRNA-1:exon:1173;Parent=Msa0097880-mRNA-1
chr1_3	CDS	3714437	3714874	3714437	ID=Msa0097880-mRNA-1:cds;Parent=Msa0097880-mRNA-1

Gene Sequence

>Msa0097880

ATGTCCTTCCATGAAGAGGAGTATCCGGTCCGGCTCACGTGTGTTCCGGTTCATGAAACCATGTTCACACAAGGCGAAATAGAGGAGTTATTTTCCCTCATTAACCAACCGGTTGATCCATCCAGTCCAGGGTCCGGTTCACAAGGATCAAGTCGTGCGGTTTACTCGACCCGTGAGAGAAAAATAAGGAGAATGCAATCAAACCGGGAGTCGGCTAGGCGGTCACGTTGGAGAAAGAAGCGGCACGTAGAGAACCTCACGAACGATTTGAACCGGTTGAGAATGGAAAACCGGGAACTAAAAAACCAGCTAGGGTTAACCATGCAATACAATCTCATATTATCAACGGAGAATGAACGTCTAAGATCAGAATCAATGTCCCTCATGGCAACACTTTTGGATCTATATCGGACTTTAGAGACAATAATTTCACAATAG

Protein sequence

>Msa0097880

MSFHEEEYPVRLTCVPVHETMFTQGEIEELFSLINQPVDPSSPGSGSQGSSRAVYSTRERKIRRMQSNRESARRSRWRKKRHVENLTNDLNRLRMENRELKNQLGLTMQYNLILSTENERLRSESMSLMATLLDLYRTLETIISQ*