AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0099840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0099840 MtrunA17_Chr1g0150481 99.219 128 1 0 1 128 1 128 8.74e-93 263
Msa0099840 MtrunA17_Chr3g0142391 96.875 128 4 0 1 128 1 128 3.06e-91 259
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0099840 AT5G39600.1 78.740 127 27 0 1 127 1 127 1.48e-71 210

Find 209 sgRNAs with CRISPR-Local

Find 313 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGACATTTACTTTGAAATTT+AGG 0.088666 1_3:+6167465 Msa0099840:five_prime_UTR
TAATTGAGATCAGGGAATTT+AGG 0.162993 1_3:+6166833 Msa0099840:five_prime_UTR
GACATTTACTTTGAAATTTA+GGG 0.205658 1_3:+6167466 Msa0099840:five_prime_UTR
AGCAGCTGCGTCGGCTTTGC+AGG 0.207136 1_3:+6167048 Msa0099840:five_prime_UTR
AATTTGAGATATCATCTTTC+AGG 0.227156 1_3:+6170311 Msa0099840:three_prime_UTR
GTATATGTTAATGATATATT+AGG 0.232906 1_3:-6165979 Msa0099830:three_prime_UTR
GCGAACCTTCCTTTAATTCT+AGG 0.237184 1_3:+6166258 Msa0099840:five_prime_UTR
TAACCACTGGGAATTGTTCT+AGG 0.247546 1_3:+6169769 Msa0099840:three_prime_UTR
GAAACTCGACCTAGAATTAA+AGG 0.252531 1_3:-6166267 Msa0099830:CDS
CCAGAATCATCTTCGTTATA+CGG 0.253037 1_3:-6168160 None:intergenic
GAATGTGTGGTTGTGGTGAT+TGG 0.270371 1_3:+6167359 Msa0099840:five_prime_UTR
CAAAATTAGCAGTAAATAAA+AGG 0.271000 1_3:+6167719 Msa0099840:five_prime_UTR
ATTTAGGGTTTGAAGGAGTT+TGG 0.272290 1_3:+6166849 Msa0099840:five_prime_UTR
AATTGAGATCAGGGAATTTA+GGG 0.282604 1_3:+6166834 Msa0099840:five_prime_UTR
AATTTCAAAGTAAATGTCTT+TGG 0.284553 1_3:-6167461 None:intergenic
AATGTGTGGTTGTGGTGATT+GGG 0.291161 1_3:+6167360 Msa0099840:five_prime_UTR
TGGGGTAAAGTATCACTATT+TGG 0.293723 1_3:+6167379 Msa0099840:five_prime_UTR
CAATGCCTACATTTGAAGTT+TGG 0.297387 1_3:+6170060 Msa0099840:three_prime_UTR
CCGTATAACGAAGATGATTC+TGG 0.317599 1_3:+6168160 Msa0099840:CDS
GTGACGAAATCGTCAATTTC+GGG 0.319268 1_3:+6166794 Msa0099840:five_prime_UTR
TGTTGCTTTGGATGACTTGC+AGG 0.340517 1_3:+6167204 Msa0099840:five_prime_UTR
AGGAATTTATACATATAATA+AGG 0.345455 1_3:+6165863 Msa0099840:five_prime_UTR
AAGGCATGGTGAAACTAAAA+AGG 0.348802 1_3:-6169979 None:intergenic
AAGAAGCAGAAGCTGCTTCC+TGG 0.355450 1_3:-6166877 Msa0099830:CDS
ATGTATAAATTCCTTTGGTT+TGG 0.360369 1_3:-6165854 Msa0099830:three_prime_UTR
TCAGGGAATTTAGGGTTTGA+AGG 0.367060 1_3:+6166842 Msa0099840:five_prime_UTR
AGTGACGAAATCGTCAATTT+CGG 0.368521 1_3:+6166793 Msa0099840:five_prime_UTR
GACGCCATCTGCGGTGGTGC+CGG 0.374473 1_3:+6166733 Msa0099840:five_prime_UTR
CACCGCAGATGGCGTCGTTC+CGG 0.376246 1_3:-6166726 Msa0099830:CDS
AACTCGATAATTCAGTTCTA+AGG 0.376645 1_3:-6169835 None:intergenic
CAATTCAATAAACAACAAAA+TGG 0.385236 1_3:-6167245 Msa0099830:exon
TTCTTAAACAAGGGAGACTA+AGG 0.386131 1_3:+6167908 Msa0099840:five_prime_UTR
ATTATATGTATAAATTCCTT+TGG 0.388023 1_3:-6165859 Msa0099830:three_prime_UTR
GTGGCGGGAGGCGAGTTTGT+TGG 0.388480 1_3:+6167120 Msa0099840:five_prime_UTR
AACGAAGATGATTCTGGAAA+AGG 0.393234 1_3:+6168166 Msa0099840:CDS
GTTGTTGTTGTGGTGGTGGA+AGG 0.394821 1_3:+6166949 Msa0099840:five_prime_UTR
GAGAGAAAAGAAAGAATAGA+AGG 0.401420 1_3:-6166332 Msa0099830:CDS
TCTTCATCAGGAATGATAAC+AGG 0.403293 1_3:-6168239 None:intergenic
GAGTGTGACGCGGTGGTGGC+GGG 0.403622 1_3:+6167105 Msa0099840:five_prime_UTR
TTCTTCTCCTCTGCTTTCCT+CGG 0.408134 1_3:-6169719 None:intergenic
TACTGGTGGCATATTCTCCT+TGG 0.408673 1_3:+6167755 Msa0099840:five_prime_UTR
TAGTGAGAGTAATTGAGATC+AGG 0.412297 1_3:+6166824 Msa0099840:five_prime_UTR
ACGGCAGAATAGTGTTGCTT+TGG 0.415438 1_3:+6167192 Msa0099840:five_prime_UTR
ACACAACAACTATAACTGTT+TGG 0.416156 1_3:-6170187 None:intergenic
CAGAGAAGAATGATTGTTGT+TGG 0.418301 1_3:+6166924 Msa0099840:five_prime_UTR
AGCTTCTGCTTCTTCGTTGG+TGG 0.421356 1_3:+6166886 Msa0099840:five_prime_UTR
GGCTGTTGTTGTTGTGGTGG+TGG 0.426525 1_3:+6166945 Msa0099840:five_prime_UTR
CCGTCTCTGTCGCCGTCGTT+TGG 0.426542 1_3:+6166418 Msa0099840:five_prime_UTR
TACTTTGAAATTTAGGGATA+GGG 0.427212 1_3:+6167472 Msa0099840:five_prime_UTR
ACGCCATCTGCGGTGGTGCC+GGG 0.428709 1_3:+6166734 Msa0099840:five_prime_UTR
AGAGTGTGACGCGGTGGTGG+CGG 0.429623 1_3:+6167104 Msa0099840:five_prime_UTR
TTCACTTCGATTAGAAAGAA+AGG 0.431350 1_3:-6166155 Msa0099830:three_prime_UTR
AACTTCATACATAGTTCAAC+AGG 0.431616 1_3:+6166176 Msa0099840:five_prime_UTR
AGTGAGAGTAATTGAGATCA+GGG 0.433298 1_3:+6166825 Msa0099840:five_prime_UTR
ATGTGTGGTTGTGGTGATTG+GGG 0.434776 1_3:+6167361 Msa0099840:five_prime_UTR
TATCACTATTTGGTGGGGTA+GGG 0.436801 1_3:+6167389 Msa0099840:five_prime_UTR
TTACTTTGAAATTTAGGGAT+AGG 0.437945 1_3:+6167471 Msa0099840:five_prime_UTR
AAAGTAAATGTCTTTGGCTA+AGG 0.439439 1_3:-6167455 None:intergenic
TCCGTCATCACGTTTCCGTC+TGG 0.440194 1_3:+6166459 Msa0099840:five_prime_UTR
CGTTCGTGGCGGCGAGCTAG+TGG 0.441707 1_3:+6166986 Msa0099840:five_prime_UTR
GTATCACTATTTGGTGGGGT+AGG 0.441747 1_3:+6167388 Msa0099840:five_prime_UTR
ACGAAGATGATTCTGGAAAA+GGG 0.442229 1_3:+6168167 Msa0099840:CDS
ATCGGAACCGCCACCGTCAT+CGG 0.447848 1_3:+6166393 Msa0099840:five_prime_UTR
TCCACGAACTGCATCATGTA+TGG 0.449429 1_3:+6167980 Msa0099840:CDS
GCAGCAGATGCGAGATAAAA+CGG 0.452597 1_3:-6169799 None:intergenic
TGGTTTAGCTTATTAACCAC+TGG 0.457433 1_3:+6169756 Msa0099840:three_prime_UTR
GCAGCTACATGGTGATCATA+TGG 0.460331 1_3:+6170220 Msa0099840:three_prime_UTR
AAGGCATCAATGTGGCATAC+TGG 0.461670 1_3:+6167738 Msa0099840:five_prime_UTR
CCGGAGCTTGTTCACAGACT+TGG 0.464846 1_3:+6167080 Msa0099840:five_prime_UTR
TTAGGTATCTTCGCCGTCGA+TGG 0.464857 1_3:-6166496 Msa0099830:intron
ATCACAGTGACATTTGTTAA+CGG 0.466405 1_3:+6168098 Msa0099840:CDS
GAACAAATGTTTCGTGAAGC+AGG 0.466679 1_3:+6168206 Msa0099840:CDS
TGGTGGTGGAAGGTGGTGAG+AGG 0.469506 1_3:+6166959 Msa0099840:five_prime_UTR
TCTCTTTCCACCGCCGTCAT+CGG 0.470887 1_3:+6166375 Msa0099840:five_prime_UTR
TTCACCTCAACTTCGCAGGC+AGG 0.472420 1_3:-6168047 None:intergenic
ATGCAACAATTTAGAACCGT+TGG 0.473284 1_3:+6170506 Msa0099840:three_prime_UTR
GAGTGTAGAAGAGGAGAGGA+GGG 0.474017 1_3:+6167337 Msa0099840:five_prime_UTR
AGCAGCTTCTGCTTCTTCGT+TGG 0.474181 1_3:+6166883 Msa0099840:five_prime_UTR
GCTTTGCAGGTGACGTGAGC+CGG 0.474575 1_3:+6167061 Msa0099840:five_prime_UTR
GGCGGTTCCGATGACGGCGG+TGG 0.476197 1_3:-6166382 Msa0099830:CDS
TGTGGTTATTTCTTAAACAA+GGG 0.477181 1_3:+6167899 Msa0099840:five_prime_UTR
ATCACTATTTGGTGGGGTAG+GGG 0.482413 1_3:+6167390 Msa0099840:five_prime_UTR
TTGCAGTACTTAGTATCATA+AGG 0.482570 1_3:-6170156 None:intergenic
GACGGTGGCGGTTCCGATGA+CGG 0.482863 1_3:-6166388 Msa0099830:CDS
AGAAAACGCACTTTGGTGCC+AGG 0.487077 1_3:-6168278 None:intergenic
ATCTTCGTTATACGGTGAAC+CGG 0.487143 1_3:-6168152 None:intergenic
TGCAACAATTTAGAACCGTT+GGG 0.489062 1_3:+6170507 Msa0099840:three_prime_UTR
TCCATACATGATGCAGTTCG+TGG 0.490045 1_3:-6167981 None:intergenic
TTGGATTGGGAGTGTAGAAG+AGG 0.490072 1_3:+6167328 Msa0099840:five_prime_UTR
GACGATTTCGTCACTGTCAA+CGG 0.491915 1_3:-6166785 Msa0099830:CDS
TTAATTGCAGAGGATGAATA+CGG 0.494889 1_3:-6166210 Msa0099830:CDS
GCCAACTCATAATTCTCTAC+TGG 0.503584 1_3:+6170458 Msa0099840:three_prime_UTR
TGAAGGAGTTTGGAGAAGCC+AGG 0.505414 1_3:+6166859 Msa0099840:five_prime_UTR
GGCAGAGTGTGACGCGGTGG+TGG 0.506125 1_3:+6167101 Msa0099840:five_prime_UTR
GTAGTGTTCGTGTTCGTGTT+CGG 0.509358 1_3:+6166679 Msa0099840:five_prime_UTR
TGTTGAGAGAGTTCTTCATC+AGG 0.509502 1_3:-6168251 None:intergenic
AACAAATGTTTCGTGAAGCA+GGG 0.509969 1_3:+6168207 Msa0099840:CDS
GTTGTTGTGGTGGTGGAAGG+TGG 0.511007 1_3:+6166952 Msa0099840:five_prime_UTR
TTGTGGTTATTTCTTAAACA+AGG 0.512854 1_3:+6167898 Msa0099840:five_prime_UTR
GGAGTGTAGAAGAGGAGAGG+AGG 0.513691 1_3:+6167336 Msa0099840:five_prime_UTR
CAGCAGATGCGAGATAAAAC+GGG 0.514142 1_3:-6169798 None:intergenic
TGGTGAGAGGGACACGTTCG+TGG 0.516914 1_3:+6166972 Msa0099840:five_prime_UTR
GTTGGCTGTTGTTGTTGTGG+TGG 0.517079 1_3:+6166942 Msa0099840:five_prime_UTR
AGGGATGCAAGGACAGAAAG+AGG 0.524843 1_3:+6167491 Msa0099840:five_prime_UTR
GGTATCTTCGCCGTCGATGG+AGG 0.526645 1_3:-6166493 Msa0099830:CDS
ATCTTGAGAGTAGCTAATTG+AGG 0.529014 1_3:+6167415 Msa0099840:five_prime_UTR
AGAGGAGAGGAGGGAATGTG+TGG 0.529036 1_3:+6167346 Msa0099840:five_prime_UTR
CTAAACCAAACTTCAAATGT+AGG 0.531759 1_3:-6170065 None:intergenic
TGAGAGTGTTGTGAGTGTGT+TGG 0.533685 1_3:+6167292 Msa0099840:five_prime_UTR
CCAAGTCTGTGAACAAGCTC+CGG 0.534270 1_3:-6167080 Msa0099830:CDS
AAATATGAATTTAATTGCAG+AGG 0.534945 1_3:-6166220 Msa0099830:CDS
TTCGTGTTCGTGTTCGGTGG+TGG 0.536195 1_3:+6166685 Msa0099840:five_prime_UTR
AGTCCCGGCACCACCGCAGA+TGG 0.542153 1_3:-6166737 Msa0099830:CDS
CGGCGGTGGAAAGAGAAGCT+AGG 0.543681 1_3:-6166368 Msa0099830:CDS
TCTTGAGAGTAGCTAATTGA+GGG 0.545062 1_3:+6167416 Msa0099840:five_prime_UTR
GTTCGTGGCGGCGAGCTAGT+GGG 0.545982 1_3:+6166987 Msa0099840:five_prime_UTR
GAGGAGGGAATGTGTGGTTG+TGG 0.546672 1_3:+6167352 Msa0099840:five_prime_UTR
GTTGTTGGCTGTTGTTGTTG+TGG 0.546859 1_3:+6166939 Msa0099840:five_prime_UTR
TCACCTCAACTTCGCAGGCA+GGG 0.547983 1_3:-6168046 None:intergenic
GGTGGTGGAAGGTGGTGAGA+GGG 0.548296 1_3:+6166960 Msa0099840:five_prime_UTR
AGGCGAGTTTGTTGGCAGCA+TGG 0.549459 1_3:+6167128 Msa0099840:five_prime_UTR
GGCAAGGATATAATAAAAGC+GGG 0.549674 1_3:-6167789 None:intergenic
ATTCAGAGTTCAAATTGAAG+AGG 0.550248 1_3:+6166543 Msa0099840:five_prime_UTR
AGAGTGGATGTCATGATCAC+AGG 0.550527 1_3:+6167015 Msa0099840:five_prime_UTR
TCTTCGCCGTCGATGGAGGT+TGG 0.551535 1_3:-6166489 Msa0099830:CDS
GTAAAGTATCACTATTTGGT+GGG 0.551859 1_3:+6167383 Msa0099840:five_prime_UTR
ACGCCGGAGCAAAACAGTCC+CGG 0.553478 1_3:-6166752 Msa0099830:CDS
CGGAACGACGCCATCTGCGG+TGG 0.553872 1_3:+6166727 Msa0099840:five_prime_UTR
AAACCCTGCCTGCGAAGTTG+AGG 0.556200 1_3:+6168043 Msa0099840:CDS
GGCGGTGGAAAGAGAAGCTA+GGG 0.558883 1_3:-6166367 Msa0099830:CDS
TTGGGAGTGTAGAAGAGGAG+AGG 0.559292 1_3:+6167333 Msa0099840:five_prime_UTR
ACGTTTCACCTCAACTTCGC+AGG 0.559908 1_3:-6168051 None:intergenic
GACGGCGCCGATGACGGTGG+CGG 0.560155 1_3:-6166400 Msa0099830:CDS
GGTTTAGCTTATTAACCACT+GGG 0.564730 1_3:+6169757 Msa0099840:three_prime_UTR
ATGATCACCATGTAGCTGCT+TGG 0.565455 1_3:-6170216 None:intergenic
CTTTGGCTAAGGCTCACCCA+CGG 0.568239 1_3:-6167444 None:intergenic
GAAACATTTGTTCAGTCTCA+AGG 0.568869 1_3:-6168196 None:intergenic
TTTGGCTAAGGCTCACCCAC+GGG 0.570733 1_3:-6167443 None:intergenic
TTAGTGTTCTTGTGATTGAG+AGG 0.572305 1_3:+6170084 Msa0099840:three_prime_UTR
ACCAGACGGAAACGTGATGA+CGG 0.572547 1_3:-6166460 Msa0099830:CDS
ACCAGTAGAGAATTATGAGT+TGG 0.574445 1_3:-6170459 None:intergenic
TAGTGTTCTTGTGATTGAGA+GGG 0.578101 1_3:+6170085 Msa0099840:three_prime_UTR
CAATAATGCAAAATTTGACA+TGG 0.578218 1_3:+6170416 Msa0099840:three_prime_UTR
TGATTGAAATTGTAAGTGAA+AGG 0.578430 1_3:+6166623 Msa0099840:five_prime_UTR
TGACAGCTTAAAACAAGGCA+TGG 0.580565 1_3:-6169993 None:intergenic
TGTGACGCGGTGGTGGCGGG+AGG 0.581988 1_3:+6167108 Msa0099840:five_prime_UTR
GATGTTCACACATAGCTTCG+TGG 0.582632 1_3:-6169952 None:intergenic
AAATAAATTTCATGTCGGCA+AGG 0.586964 1_3:-6167805 None:intergenic
TTTGTTGGCAGCATGGATGT+TGG 0.587996 1_3:+6167135 Msa0099840:five_prime_UTR
CACCGGAACGACGCCATCTG+CGG 0.589463 1_3:+6166724 Msa0099840:five_prime_UTR
TAATGCAAAATTTGACATGG+TGG 0.589856 1_3:+6170419 Msa0099840:three_prime_UTR
GACAGAGACGGCGCCGATGA+CGG 0.593724 1_3:-6166406 Msa0099830:CDS
TTCGTGAAGCAGGGGAACAA+TGG 0.596028 1_3:+6168216 Msa0099840:CDS
ATTGAGAGGGTGATTTCTGT+CGG 0.602555 1_3:+6170098 Msa0099840:three_prime_UTR
GAACTCTCTCAACATGCACC+TGG 0.603714 1_3:+6168260 Msa0099840:CDS
CCAAACGACGGCGACAGAGA+CGG 0.604022 1_3:-6166418 Msa0099830:CDS
TGGAATCACATGTGAGACAA+AGG 0.606070 1_3:+6167155 Msa0099840:five_prime_UTR
AGCGCAATGCAACTCACGAA+AGG 0.606591 1_3:+6168010 Msa0099840:CDS
AGGGACTCTTCTGAGATGAG+TGG 0.606657 1_3:+6167511 Msa0099840:five_prime_UTR
TAAAGTATCACTATTTGGTG+GGG 0.607778 1_3:+6167384 Msa0099840:five_prime_UTR
GGTAAAGTATCACTATTTGG+TGG 0.608011 1_3:+6167382 Msa0099840:five_prime_UTR
CTTCCAGCCGAGGAAAGCAG+AGG 0.610495 1_3:+6169712 Msa0099840:intron
ACTCAACCTTCACTCTCGAT+AGG 0.611470 1_3:-6167944 None:intergenic
AAAACAAATAAATTTCATGT+CGG 0.611849 1_3:-6167810 None:intergenic
CGGCAAGGATATAATAAAAG+CGG 0.612511 1_3:-6167790 None:intergenic
TGACATGGTGGAAATTAATG+TGG 0.614093 1_3:+6170431 Msa0099840:three_prime_UTR
ACAAACAATCTCAAAATACA+TGG 0.615194 1_3:-6170378 None:intergenic
GATACATAGAGCAGCTGCGT+CGG 0.618915 1_3:+6167039 Msa0099840:five_prime_UTR
TTTGATGCCAAGCAGCTACA+TGG 0.619235 1_3:+6170209 Msa0099840:three_prime_UTR
GCTAATTGAGGGATGACCCG+TGG 0.620463 1_3:+6167427 Msa0099840:five_prime_UTR
TAATCCAAACTCCAAACCAA+AGG 0.621247 1_3:+6165843 None:intergenic
CTAATTGAGGGATGACCCGT+GGG 0.625448 1_3:+6167428 Msa0099840:five_prime_UTR
CTCGACCTAGAATTAAAGGA+AGG 0.631588 1_3:-6166263 Msa0099830:CDS
AAAGGAAAGCTGAATCAGAA+CGG 0.631900 1_3:+6167173 Msa0099840:five_prime_UTR
GAAGAAGCAATAGCTAACAA+TGG 0.632396 1_3:+6169736 Msa0099840:exon
AATTTAGGGATAGGGATGCA+AGG 0.632979 1_3:+6167480 Msa0099840:five_prime_UTR
AGATAAAATTCATTACCCAA+CGG 0.635918 1_3:-6170522 None:intergenic
ACAACTCCAACCTCCATCGA+CGG 0.637687 1_3:+6166483 Msa0099840:five_prime_UTR
GTGTTCGTGTTCGTGTTCGG+TGG 0.637730 1_3:+6166682 Msa0099840:five_prime_UTR
GCATCAATGTGGCATACTGG+TGG 0.639001 1_3:+6167741 Msa0099840:five_prime_UTR
AGCGGGTAAAGCGAAATCCA+AGG 0.640084 1_3:-6167772 None:intergenic
GAGCTAGTGGGTTAGCAGAG+TGG 0.641063 1_3:+6166999 Msa0099840:five_prime_UTR
GGGATGCAAGGACAGAAAGA+GGG 0.642108 1_3:+6167492 Msa0099840:five_prime_UTR
TGAGGTGAAACGTGGAAATG+TGG 0.643314 1_3:+6168061 Msa0099840:CDS
GAAATTCCTATCGAGAGTGA+AGG 0.644033 1_3:+6167938 Msa0099840:CDS
ACAAATGTTTCGTGAAGCAG+GGG 0.647258 1_3:+6168208 Msa0099840:CDS
AATGTCACTGTGATCTGTGG+TGG 0.647419 1_3:-6168089 None:intergenic
ATGTCACTGTGATCTGTGGT+GGG 0.660137 1_3:-6168088 None:intergenic
AAAATATTAACCTAAGCACA+AGG 0.661387 1_3:+6170260 Msa0099840:three_prime_UTR
TTTCGATGCAACATCAACGC+CGG 0.666295 1_3:+6168133 Msa0099840:CDS
TTCAGTTCTAAGGAACAGCA+CGG 0.667181 1_3:-6169825 None:intergenic
TCATTTGACAGCTTAAAACA+AGG 0.670741 1_3:-6169998 None:intergenic
AGTGACATTTGTTAACGGTG+TGG 0.673167 1_3:+6168103 Msa0099840:CDS
TCATACATAGTTCAACAGGA+AGG 0.676360 1_3:+6166180 Msa0099840:five_prime_UTR
AGAAAACACAACCTACACCA+AGG 0.676693 1_3:-6167836 None:intergenic
ACAAATGTCACTGTGATCTG+TGG 0.681462 1_3:-6168092 None:intergenic
AGAGACGGCGCCGATGACGG+TGG 0.685333 1_3:-6166403 Msa0099830:CDS
GAGGTTGGAGTTGTACCAGA+CGG 0.695762 1_3:-6166474 Msa0099830:CDS
TGCGAAGTTGAGGTGAAACG+TGG 0.696804 1_3:+6168053 Msa0099840:CDS
GTAAATAAAAGGCATCAATG+TGG 0.701494 1_3:+6167730 Msa0099840:five_prime_UTR
TGAGAGGGACACGTTCGTGG+CGG 0.705181 1_3:+6166975 Msa0099840:five_prime_UTR
CAACGGTAAAACAGACACGC+CGG 0.705434 1_3:-6166768 Msa0099830:CDS
CTTGGCAGAGTGTGACGCGG+TGG 0.706255 1_3:+6167098 Msa0099840:five_prime_UTR
AGACTTGGCAGAGTGTGACG+CGG 0.706454 1_3:+6167095 Msa0099840:five_prime_UTR
TGACGAAATCGTCAATTTCG+GGG 0.717237 1_3:+6166795 Msa0099840:five_prime_UTR
AAACCTAGAACAATTCCCAG+TGG 0.754435 1_3:-6169772 None:intergenic
GTACTGCGGTTACCAAACGA+CGG 0.762753 1_3:-6166430 Msa0099830:CDS
GGTGGCGGTTCCGATGACGG+CGG 0.772550 1_3:-6166385 Msa0099830:CDS
ATGACGGAGATATCGTACTG+CGG 0.812252 1_3:-6166444 Msa0099830:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAGGTTAATATTTTAAATT+GGG - chr1_3:6170255-6170274 None:intergenic 10.0%
!! ATTATATGTATAAATTCCTT+TGG - chr1_3:6165862-6165881 Msa0099830:three_prime_UTR 15.0%
!! AGGAATTTATACATATAATA+AGG + chr1_3:6165863-6165882 Msa0099840:five_prime_UTR 15.0%
!! GTATATGTTAATGATATATT+AGG - chr1_3:6165982-6166001 Msa0099830:three_prime_UTR 15.0%
!!! ATCGTAATTTTTTTGAATTT+TGG + chr1_3:6166581-6166600 Msa0099840:five_prime_UTR 15.0%
!! TCCTAGAAAATAAAAATAAA+AGG - chr1_3:6167667-6167686 None:intergenic 15.0%
!! AAAACAAATAAATTTCATGT+CGG - chr1_3:6167813-6167832 None:intergenic 15.0%
!!! AATTTATTTGTTTTTTTCCT+TGG + chr1_3:6167819-6167838 Msa0099840:five_prime_UTR 15.0%
!!! TTTTATATACACTTTAATTG+TGG + chr1_3:6167881-6167900 Msa0099840:five_prime_UTR 15.0%
!! ATTTACCTAAAGAATTATTA+AGG - chr1_3:6168519-6168538 None:intergenic 15.0%
!! AATAATTCTTTAGGTAAATT+AGG + chr1_3:6168520-6168539 Msa0099840:intron 15.0%
!!! CTAAATGTTTCTATAATAAT+TGG + chr1_3:6169475-6169494 Msa0099840:intron 15.0%
!!! ATAATAATTGGAATATGTTT+TGG + chr1_3:6169487-6169506 Msa0099840:intron 15.0%
!!! CTTAGGTTAATATTTTAAAT+TGG - chr1_3:6170256-6170275 None:intergenic 15.0%
!! AAATATGAATTTAATTGCAG+AGG - chr1_3:6166223-6166242 Msa0099830:CDS 20.0%
!!! ATTTCTTTTTTGTGTTGTTT+AGG - chr1_3:6166517-6166536 Msa0099830:intron 20.0%
!! CAATTCAATAAACAACAAAA+TGG - chr1_3:6167248-6167267 Msa0099830:exon 20.0%
!! AATTTCAAAGTAAATGTCTT+TGG - chr1_3:6167464-6167483 None:intergenic 20.0%
!!! AGACATTTACTTTGAAATTT+AGG + chr1_3:6167465-6167484 Msa0099840:five_prime_UTR 20.0%
!!! GACATTTACTTTGAAATTTA+GGG + chr1_3:6167466-6167485 Msa0099840:five_prime_UTR 20.0%
!!! GCCTTTTATTTTTATTTTCT+AGG + chr1_3:6167663-6167682 Msa0099840:five_prime_UTR 20.0%
!!! CTTTTATTTACTGCTAATTT+TGG - chr1_3:6167721-6167740 None:intergenic 20.0%
!! CAAAATTAGCAGTAAATAAA+AGG + chr1_3:6167719-6167738 Msa0099840:five_prime_UTR 20.0%
!! TGTTACCTTAATAATTCTTT+AGG + chr1_3:6168511-6168530 Msa0099840:intron 20.0%
!!! GTTAAGTAATAAATGGATTA+AGG - chr1_3:6169190-6169209 None:intergenic 20.0%
!! AATGCTAGTTAAGTAATAAA+TGG - chr1_3:6169197-6169216 None:intergenic 20.0%
!! ATCAAGATATTATCTTAGTA+TGG + chr1_3:6169237-6169256 Msa0099840:intron 20.0%
!! AAATAGTAATTACAGTACAT+TGG + chr1_3:6169598-6169617 Msa0099840:intron 20.0%
!!! ACTATTTTGCTATTTTGAAA+AGG + chr1_3:6170339-6170358 Msa0099840:three_prime_UTR 20.0%
! ATGTATAAATTCCTTTGGTT+TGG - chr1_3:6165857-6165876 Msa0099830:three_prime_UTR 25.0%
! ACTTGAAAAACATTCAAACA+CGG + chr1_3:6166131-6166150 Msa0099840:five_prime_UTR 25.0%
! TGATTGAAATTGTAAGTGAA+AGG + chr1_3:6166623-6166642 Msa0099840:five_prime_UTR 25.0%
!!! TGAAGTTTTGTAATTGGATT+GGG + chr1_3:6167315-6167334 Msa0099840:five_prime_UTR 25.0%
!!! TTACTTTGAAATTTAGGGAT+AGG + chr1_3:6167471-6167490 Msa0099840:five_prime_UTR 25.0%
!!! TACTTTGAAATTTAGGGATA+GGG + chr1_3:6167472-6167491 Msa0099840:five_prime_UTR 25.0%
!!! TTGTGGTTATTTCTTAAACA+AGG + chr1_3:6167898-6167917 Msa0099840:five_prime_UTR 25.0%
!!! TGTGGTTATTTCTTAAACAA+GGG + chr1_3:6167899-6167918 Msa0099840:five_prime_UTR 25.0%
! ATGACAAGAAAACATGAATA+AGG - chr1_3:6168316-6168335 None:intergenic 25.0%
!!! ATTTTTATTGGCTTAAATCG+TGG + chr1_3:6168877-6168896 Msa0099840:intron 25.0%
! ATTCCTCTTTGTAGAATATA+AGG + chr1_3:6169012-6169031 Msa0099840:intron 25.0%
! TCCATTTCATACAATAATAC+AGG - chr1_3:6169346-6169365 None:intergenic 25.0%
!! AACAAAGAGTACAATCAATT+TGG - chr1_3:6169402-6169421 None:intergenic 25.0%
!!! ACTCTTTGTTGTTTTAGTTA+TGG + chr1_3:6169412-6169431 Msa0099840:intron 25.0%
!!! AAACATTTAGTTCTTCTAAC+CGG - chr1_3:6169465-6169484 None:intergenic 25.0%
!!! ATAATTGGAATATGTTTTGG+AGG + chr1_3:6169490-6169509 Msa0099840:intron 25.0%
!! ATCTCTGTTTAATTTACTTG+TGG + chr1_3:6169673-6169692 Msa0099840:intron 25.0%
!! TCTCTGTTTAATTTACTTGT+GGG + chr1_3:6169674-6169693 Msa0099840:intron 25.0%
!! GTCAAATGATATCATTTTGT+TGG + chr1_3:6170012-6170031 Msa0099840:three_prime_UTR 25.0%
! AAAATATTAACCTAAGCACA+AGG + chr1_3:6170260-6170279 Msa0099840:three_prime_UTR 25.0%
!! AATTTGAGATATCATCTTTC+AGG + chr1_3:6170311-6170330 Msa0099840:three_prime_UTR 25.0%
! ACAAACAATCTCAAAATACA+TGG - chr1_3:6170381-6170400 None:intergenic 25.0%
!!! ATGTATTTTGAGATTGTTTG+TGG + chr1_3:6170380-6170399 Msa0099840:three_prime_UTR 25.0%
! CAATAATGCAAAATTTGACA+TGG + chr1_3:6170416-6170435 Msa0099840:three_prime_UTR 25.0%
TTCACTTCGATTAGAAAGAA+AGG - chr1_3:6166158-6166177 Msa0099830:three_prime_UTR 30.0%
AACTTCATACATAGTTCAAC+AGG + chr1_3:6166176-6166195 Msa0099840:five_prime_UTR 30.0%
TTAATTGCAGAGGATGAATA+CGG - chr1_3:6166213-6166232 Msa0099830:CDS 30.0%
GAGAGAAAAGAAAGAATAGA+AGG - chr1_3:6166335-6166354 Msa0099830:CDS 30.0%
ATTCAGAGTTCAAATTGAAG+AGG + chr1_3:6166543-6166562 Msa0099840:five_prime_UTR 30.0%
! TTTTGGATTTCTCGTTCTTA+CGG + chr1_3:6166598-6166617 Msa0099840:five_prime_UTR 30.0%
TAATTGAGATCAGGGAATTT+AGG + chr1_3:6166833-6166852 Msa0099840:five_prime_UTR 30.0%
AATTGAGATCAGGGAATTTA+GGG + chr1_3:6166834-6166853 Msa0099840:five_prime_UTR 30.0%
!! TGTTGGTGAAGTTTTGTAAT+TGG + chr1_3:6167309-6167328 Msa0099840:five_prime_UTR 30.0%
!! GTGAAGTTTTGTAATTGGAT+TGG + chr1_3:6167314-6167333 Msa0099840:five_prime_UTR 30.0%
! GTAAAGTATCACTATTTGGT+GGG + chr1_3:6167383-6167402 Msa0099840:five_prime_UTR 30.0%
! TAAAGTATCACTATTTGGTG+GGG + chr1_3:6167384-6167403 Msa0099840:five_prime_UTR 30.0%
AAAGTAAATGTCTTTGGCTA+AGG - chr1_3:6167458-6167477 None:intergenic 30.0%
!! GTAAATAAAAGGCATCAATG+TGG + chr1_3:6167730-6167749 Msa0099840:five_prime_UTR 30.0%
AAATAAATTTCATGTCGGCA+AGG - chr1_3:6167808-6167827 None:intergenic 30.0%
!!! TTTGTTTTTTTCCTTGGTGT+AGG + chr1_3:6167825-6167844 Msa0099840:five_prime_UTR 30.0%
! ATCACAGTGACATTTGTTAA+CGG + chr1_3:6168098-6168117 Msa0099840:CDS 30.0%
AAAATGAAGAAAACGCACTT+TGG - chr1_3:6168288-6168307 None:intergenic 30.0%
TACCACTGGTAATAATAAGT+AGG + chr1_3:6168559-6168578 Msa0099840:intron 30.0%
TAATAAGTAACCACATGTGT+CGG + chr1_3:6168648-6168667 Msa0099840:intron 30.0%
! TCCAACACTCACATTTTTAT+TGG + chr1_3:6168865-6168884 Msa0099840:intron 30.0%
GAAATGAGTTTCATTCAAAG+TGG + chr1_3:6168916-6168935 Msa0099840:intron 30.0%
TCAACACTCATTCTCTTATT+GGG - chr1_3:6168962-6168981 None:intergenic 30.0%
TTCAACACTCATTCTCTTAT+TGG - chr1_3:6168963-6168982 None:intergenic 30.0%
AGATTAGGTTAAGTTTGTGT+TGG + chr1_3:6169044-6169063 Msa0099840:intron 30.0%
GCCTGTATTATTGTATGAAA+TGG + chr1_3:6169342-6169361 Msa0099840:intron 30.0%
!!! ATGGTTTTTAAGATGAGTTG+AGG + chr1_3:6169372-6169391 Msa0099840:intron 30.0%
GGAAATGTTTGTATGTTATG+TGG + chr1_3:6169639-6169658 Msa0099840:intron 30.0%
AACTCGATAATTCAGTTCTA+AGG - chr1_3:6169838-6169857 None:intergenic 30.0%
TCATTTGACAGCTTAAAACA+AGG - chr1_3:6170001-6170020 None:intergenic 30.0%
CTAAACCAAACTTCAAATGT+AGG - chr1_3:6170068-6170087 None:intergenic 30.0%
TTGCAGTACTTAGTATCATA+AGG - chr1_3:6170159-6170178 None:intergenic 30.0%
ACACAACAACTATAACTGTT+TGG - chr1_3:6170190-6170209 None:intergenic 30.0%
TAATGCAAAATTTGACATGG+TGG + chr1_3:6170419-6170438 Msa0099840:three_prime_UTR 30.0%
TCATACATAGTTCAACAGGA+AGG + chr1_3:6166180-6166199 Msa0099840:five_prime_UTR 35.0%
GAAACTCGACCTAGAATTAA+AGG - chr1_3:6166270-6166289 Msa0099830:CDS 35.0%
! AAAGAGAAGCTAGGGTTTTA+AGG - chr1_3:6166362-6166381 Msa0099830:CDS 35.0%
!!! GTAAGTGAAAGGTTTTGAGT+TGG + chr1_3:6166634-6166653 Msa0099840:five_prime_UTR 35.0%
AGTGACGAAATCGTCAATTT+CGG + chr1_3:6166793-6166812 Msa0099840:five_prime_UTR 35.0%
TAGTGAGAGTAATTGAGATC+AGG + chr1_3:6166824-6166843 Msa0099840:five_prime_UTR 35.0%
AGTGAGAGTAATTGAGATCA+GGG + chr1_3:6166825-6166844 Msa0099840:five_prime_UTR 35.0%
! ATTTAGGGTTTGAAGGAGTT+TGG + chr1_3:6166849-6166868 Msa0099840:five_prime_UTR 35.0%
CAGAGAAGAATGATTGTTGT+TGG + chr1_3:6166924-6166943 Msa0099840:five_prime_UTR 35.0%
AAAGGAAAGCTGAATCAGAA+CGG + chr1_3:6167173-6167192 Msa0099840:five_prime_UTR 35.0%
! TGGGGTAAAGTATCACTATT+TGG + chr1_3:6167379-6167398 Msa0099840:five_prime_UTR 35.0%
! GGTAAAGTATCACTATTTGG+TGG + chr1_3:6167382-6167401 Msa0099840:five_prime_UTR 35.0%
ATCTTGAGAGTAGCTAATTG+AGG + chr1_3:6167415-6167434 Msa0099840:five_prime_UTR 35.0%
TCTTGAGAGTAGCTAATTGA+GGG + chr1_3:6167416-6167435 Msa0099840:five_prime_UTR 35.0%
GGCAAGGATATAATAAAAGC+GGG - chr1_3:6167792-6167811 None:intergenic 35.0%
CGGCAAGGATATAATAAAAG+CGG - chr1_3:6167793-6167812 None:intergenic 35.0%
! CTGTTTTCTTGCATAATAGC+AGG + chr1_3:6167857-6167876 Msa0099840:five_prime_UTR 35.0%
TTCTTAAACAAGGGAGACTA+AGG + chr1_3:6167908-6167927 Msa0099840:five_prime_UTR 35.0%
CCAGAATCATCTTCGTTATA+CGG - chr1_3:6168163-6168182 None:intergenic 35.0%
! AACGAAGATGATTCTGGAAA+AGG + chr1_3:6168166-6168185 Msa0099840:CDS 35.0%
! ACGAAGATGATTCTGGAAAA+GGG + chr1_3:6168167-6168186 Msa0099840:CDS 35.0%
GAAACATTTGTTCAGTCTCA+AGG - chr1_3:6168199-6168218 None:intergenic 35.0%
AACAAATGTTTCGTGAAGCA+GGG + chr1_3:6168207-6168226 Msa0099840:CDS 35.0%
TCTTCATCAGGAATGATAAC+AGG - chr1_3:6168242-6168261 None:intergenic 35.0%
AACGTGAATCAGAACAAATG+AGG - chr1_3:6168394-6168413 None:intergenic 35.0%
TCTGATTCACGTTTCTAACA+TGG + chr1_3:6168401-6168420 Msa0099840:intron 35.0%
CTATGATGTACAAATACCAC+TGG + chr1_3:6168545-6168564 Msa0099840:intron 35.0%
CTCCTACTTATTATTACCAG+TGG - chr1_3:6168564-6168583 None:intergenic 35.0%
CACTGGTAATAATAAGTAGG+AGG + chr1_3:6168562-6168581 Msa0099840:intron 35.0%
GTTTGGTATCTGTACTTCAT+AGG - chr1_3:6168589-6168608 None:intergenic 35.0%
!! GTGTCGGTGTTACATAAATT+AGG + chr1_3:6168725-6168744 Msa0099840:intron 35.0%
!! TTTGTTTGCGTTTTAGTGGA+TGG + chr1_3:6168787-6168806 Msa0099840:intron 35.0%
GCCAATAAAAATGTGAGTGT+TGG - chr1_3:6168869-6168888 None:intergenic 35.0%
!! TTTATTGGCTTAAATCGTGG+TGG + chr1_3:6168880-6168899 Msa0099840:intron 35.0%
ATGAGTTTCATTCAAAGTGG+TGG + chr1_3:6168919-6168938 Msa0099840:intron 35.0%
TGAGTTTCATTCAAAGTGGT+GGG + chr1_3:6168920-6168939 Msa0099840:intron 35.0%
TTCTACAAAGAGGAATGCTA+GGG - chr1_3:6169008-6169027 None:intergenic 35.0%
ATTCTACAAAGAGGAATGCT+AGG - chr1_3:6169009-6169028 None:intergenic 35.0%
CCACCTTATATTCTACAAAG+AGG - chr1_3:6169018-6169037 None:intergenic 35.0%
CCTCTTTGTAGAATATAAGG+TGG + chr1_3:6169015-6169034 Msa0099840:intron 35.0%
ATAAGGTGGTTCAAGAGATT+AGG + chr1_3:6169029-6169048 Msa0099840:intron 35.0%
TTGGATATGTCCATAAACTG+TGG - chr1_3:6169075-6169094 None:intergenic 35.0%
GATGTCTGGAAGAACATATT+TGG - chr1_3:6169160-6169179 None:intergenic 35.0%
ATTAAGGATAAGCAGATGTC+TGG - chr1_3:6169174-6169193 None:intergenic 35.0%
!!! ATATGTTTTGGAGGAATTCG+AGG + chr1_3:6169499-6169518 Msa0099840:intron 35.0%
GAATTCGAGGTTTCACTATA+AGG + chr1_3:6169512-6169531 Msa0099840:intron 35.0%
!! GGAACTGTTGTGTATTTTTG+TGG + chr1_3:6169533-6169552 Msa0099840:intron 35.0%
GAAGAAGCAATAGCTAACAA+TGG + chr1_3:6169736-6169755 Msa0099840:exon 35.0%
! TGGTTTAGCTTATTAACCAC+TGG + chr1_3:6169756-6169775 Msa0099840:three_prime_UTR 35.0%
! GGTTTAGCTTATTAACCACT+GGG + chr1_3:6169757-6169776 Msa0099840:three_prime_UTR 35.0%
ATGGTGAAACTAAAAAGGCT+AGG - chr1_3:6169977-6169996 None:intergenic 35.0%
AAGGCATGGTGAAACTAAAA+AGG - chr1_3:6169982-6170001 None:intergenic 35.0%
! CAATGCCTACATTTGAAGTT+TGG + chr1_3:6170060-6170079 Msa0099840:three_prime_UTR 35.0%
TTAGTGTTCTTGTGATTGAG+AGG + chr1_3:6170084-6170103 Msa0099840:three_prime_UTR 35.0%
TAGTGTTCTTGTGATTGAGA+GGG + chr1_3:6170085-6170104 Msa0099840:three_prime_UTR 35.0%
! TTTCAGTTTTCCTTGTGCTT+AGG - chr1_3:6170273-6170292 None:intergenic 35.0%
!!! TTTTGAGATTGTTTGTGGCT+TGG + chr1_3:6170385-6170404 Msa0099840:three_prime_UTR 35.0%
! TGACATGGTGGAAATTAATG+TGG + chr1_3:6170431-6170450 Msa0099840:three_prime_UTR 35.0%
ACCAGTAGAGAATTATGAGT+TGG - chr1_3:6170462-6170481 None:intergenic 35.0%
! ATGCAACAATTTAGAACCGT+TGG + chr1_3:6170506-6170525 Msa0099840:three_prime_UTR 35.0%
! TGCAACAATTTAGAACCGTT+GGG + chr1_3:6170507-6170526 Msa0099840:three_prime_UTR 35.0%
GCGAACCTTCCTTTAATTCT+AGG + chr1_3:6166258-6166277 Msa0099840:five_prime_UTR 40.0%
CTCGACCTAGAATTAAAGGA+AGG - chr1_3:6166266-6166285 Msa0099830:CDS 40.0%
GTGACGAAATCGTCAATTTC+GGG + chr1_3:6166794-6166813 Msa0099840:five_prime_UTR 40.0%
TGACGAAATCGTCAATTTCG+GGG + chr1_3:6166795-6166814 Msa0099840:five_prime_UTR 40.0%
! TCAGGGAATTTAGGGTTTGA+AGG + chr1_3:6166842-6166861 Msa0099840:five_prime_UTR 40.0%
TGGAATCACATGTGAGACAA+AGG + chr1_3:6167155-6167174 Msa0099840:five_prime_UTR 40.0%
AATGTGTGGTTGTGGTGATT+GGG + chr1_3:6167360-6167379 Msa0099840:five_prime_UTR 40.0%
! TATCACTATTTGGTGGGGTA+GGG + chr1_3:6167389-6167408 Msa0099840:five_prime_UTR 40.0%
AATTTAGGGATAGGGATGCA+AGG + chr1_3:6167480-6167499 Msa0099840:five_prime_UTR 40.0%
AGAAAACACAACCTACACCA+AGG - chr1_3:6167839-6167858 None:intergenic 40.0%
GAAATTCCTATCGAGAGTGA+AGG + chr1_3:6167938-6167957 Msa0099840:CDS 40.0%
ACAAATGTCACTGTGATCTG+TGG - chr1_3:6168095-6168114 None:intergenic 40.0%
! AGTGACATTTGTTAACGGTG+TGG + chr1_3:6168103-6168122 Msa0099840:CDS 40.0%
ATCTTCGTTATACGGTGAAC+CGG - chr1_3:6168155-6168174 None:intergenic 40.0%
CCGTATAACGAAGATGATTC+TGG + chr1_3:6168160-6168179 Msa0099840:CDS 40.0%
GAACAAATGTTTCGTGAAGC+AGG + chr1_3:6168206-6168225 Msa0099840:CDS 40.0%
ACAAATGTTTCGTGAAGCAG+GGG + chr1_3:6168208-6168227 Msa0099840:CDS 40.0%
TGTTGAGAGAGTTCTTCATC+AGG - chr1_3:6168254-6168273 None:intergenic 40.0%
CAATGTAGCTGAGAATGAGT+TGG + chr1_3:6168467-6168486 Msa0099840:intron 40.0%
CATTGTCGATGTGTTGTGTT+TGG - chr1_3:6168606-6168625 None:intergenic 40.0%
CAAACACAACACATCGACAA+TGG + chr1_3:6168604-6168623 Msa0099840:intron 40.0%
TCAAACACACCCTTAATGTG+AGG + chr1_3:6168703-6168722 Msa0099840:intron 40.0%
! GTCTGAGTGTTATTGTTGAG+AGG + chr1_3:6168759-6168778 Msa0099840:intron 40.0%
!!! CTGCTTTGTTTGCGTTTTAG+TGG + chr1_3:6168783-6168802 Msa0099840:intron 40.0%
!! GTTGGTTTGACCACAGTTTA+TGG + chr1_3:6169062-6169081 Msa0099840:intron 40.0%
GGACATATCCAAGCTATTGT+CGG + chr1_3:6169083-6169102 Msa0099840:intron 40.0%
TGTATGAAATGGAAGAGCGA+TGG + chr1_3:6169353-6169372 Msa0099840:intron 40.0%
TCCATGTGCATCATAAGTTG+TGG + chr1_3:6169436-6169455 Msa0099840:intron 40.0%
CCATGTGCATCATAAGTTGT+GGG + chr1_3:6169437-6169456 Msa0099840:intron 40.0%
TTGGCAGTGTAGTTGTTCTA+TGG + chr1_3:6169617-6169636 Msa0099840:intron 40.0%
TGGCAGTGTAGTTGTTCTAT+GGG + chr1_3:6169618-6169637 Msa0099840:intron 40.0%
TAACCACTGGGAATTGTTCT+AGG + chr1_3:6169769-6169788 Msa0099840:three_prime_UTR 40.0%
AAACCTAGAACAATTCCCAG+TGG - chr1_3:6169775-6169794 None:intergenic 40.0%
!! TTCAGTTCTAAGGAACAGCA+CGG - chr1_3:6169828-6169847 None:intergenic 40.0%
TGACAGCTTAAAACAAGGCA+TGG - chr1_3:6169996-6170015 None:intergenic 40.0%
ATTGAGAGGGTGATTTCTGT+CGG + chr1_3:6170098-6170117 Msa0099840:three_prime_UTR 40.0%
GCCAACTCATAATTCTCTAC+TGG + chr1_3:6170458-6170477 Msa0099840:three_prime_UTR 40.0%
! ATGACGGAGATATCGTACTG+CGG - chr1_3:6166447-6166466 Msa0099830:CDS 45.0%
GTAGTGTTCGTGTTCGTGTT+CGG + chr1_3:6166679-6166698 Msa0099840:five_prime_UTR 45.0%
GACGATTTCGTCACTGTCAA+CGG - chr1_3:6166788-6166807 Msa0099830:CDS 45.0%
! GTTGTTGGCTGTTGTTGTTG+TGG + chr1_3:6166939-6166958 Msa0099840:five_prime_UTR 45.0%
AGAGTGGATGTCATGATCAC+AGG + chr1_3:6167015-6167034 Msa0099840:five_prime_UTR 45.0%
TTTGTTGGCAGCATGGATGT+TGG + chr1_3:6167135-6167154 Msa0099840:five_prime_UTR 45.0%
!! ACGGCAGAATAGTGTTGCTT+TGG + chr1_3:6167192-6167211 Msa0099840:five_prime_UTR 45.0%
!! TGTTGCTTTGGATGACTTGC+AGG + chr1_3:6167204-6167223 Msa0099840:five_prime_UTR 45.0%
!! TGAGAGTGTTGTGAGTGTGT+TGG + chr1_3:6167292-6167311 Msa0099840:five_prime_UTR 45.0%
! TTGGATTGGGAGTGTAGAAG+AGG + chr1_3:6167328-6167347 Msa0099840:five_prime_UTR 45.0%
GAATGTGTGGTTGTGGTGAT+TGG + chr1_3:6167359-6167378 Msa0099840:five_prime_UTR 45.0%
ATGTGTGGTTGTGGTGATTG+GGG + chr1_3:6167361-6167380 Msa0099840:five_prime_UTR 45.0%
! GTATCACTATTTGGTGGGGT+AGG + chr1_3:6167388-6167407 Msa0099840:five_prime_UTR 45.0%
! ATCACTATTTGGTGGGGTAG+GGG + chr1_3:6167390-6167409 Msa0099840:five_prime_UTR 45.0%
! AAGGCATCAATGTGGCATAC+TGG + chr1_3:6167738-6167757 Msa0099840:five_prime_UTR 45.0%
TACTGGTGGCATATTCTCCT+TGG + chr1_3:6167755-6167774 Msa0099840:five_prime_UTR 45.0%
ACTCAACCTTCACTCTCGAT+AGG - chr1_3:6167947-6167966 None:intergenic 45.0%
TCCACGAACTGCATCATGTA+TGG + chr1_3:6167980-6167999 Msa0099840:CDS 45.0%
TCCATACATGATGCAGTTCG+TGG - chr1_3:6167984-6168003 None:intergenic 45.0%
TGAGGTGAAACGTGGAAATG+TGG + chr1_3:6168061-6168080 Msa0099840:CDS 45.0%
ATGTCACTGTGATCTGTGGT+GGG - chr1_3:6168091-6168110 None:intergenic 45.0%
AATGTCACTGTGATCTGTGG+TGG - chr1_3:6168092-6168111 None:intergenic 45.0%
TTTCGATGCAACATCAACGC+CGG + chr1_3:6168133-6168152 Msa0099840:CDS 45.0%
TGTCGGACATCAAAATGCGT+CGG + chr1_3:6168671-6168690 Msa0099840:intron 45.0%
ACACCCTTAATGTGAGGTGT+CGG + chr1_3:6168709-6168728 Msa0099840:intron 45.0%
ACACCGACACCTCACATTAA+GGG - chr1_3:6168715-6168734 None:intergenic 45.0%
AACACCGACACCTCACATTA+AGG - chr1_3:6168716-6168735 None:intergenic 45.0%
!! CAAAGTGGTGGGTCATACAA+TGG + chr1_3:6168931-6168950 Msa0099840:intron 45.0%
AACGACTTCCGACAATAGCT+TGG - chr1_3:6169094-6169113 None:intergenic 45.0%
CCCACAACTTATGATGCACA+TGG - chr1_3:6169440-6169459 None:intergenic 45.0%
GCATCATAAGTTGTGGGATC+CGG + chr1_3:6169443-6169462 Msa0099840:intron 45.0%
TTCTTCTCCTCTGCTTTCCT+CGG - chr1_3:6169722-6169741 None:intergenic 45.0%
CAGCAGATGCGAGATAAAAC+GGG - chr1_3:6169801-6169820 None:intergenic 45.0%
GCAGCAGATGCGAGATAAAA+CGG - chr1_3:6169802-6169821 None:intergenic 45.0%
GATGTTCACACATAGCTTCG+TGG - chr1_3:6169955-6169974 None:intergenic 45.0%
TTTGATGCCAAGCAGCTACA+TGG + chr1_3:6170209-6170228 Msa0099840:three_prime_UTR 45.0%
ATGATCACCATGTAGCTGCT+TGG - chr1_3:6170219-6170238 None:intergenic 45.0%
GCAGCTACATGGTGATCATA+TGG + chr1_3:6170220-6170239 Msa0099840:three_prime_UTR 45.0%
GTACTGCGGTTACCAAACGA+CGG - chr1_3:6166433-6166452 Msa0099830:CDS 50.0%
ACCAGACGGAAACGTGATGA+CGG - chr1_3:6166463-6166482 Msa0099830:CDS 50.0%
GAGGTTGGAGTTGTACCAGA+CGG - chr1_3:6166477-6166496 Msa0099830:CDS 50.0%
ACAACTCCAACCTCCATCGA+CGG + chr1_3:6166483-6166502 Msa0099840:five_prime_UTR 50.0%
TTAGGTATCTTCGCCGTCGA+TGG - chr1_3:6166499-6166518 Msa0099830:intron 50.0%
!!! GTTTTGCTCTGAGACTGCAC+CGG + chr1_3:6166707-6166726 Msa0099840:five_prime_UTR 50.0%
CAACGGTAAAACAGACACGC+CGG - chr1_3:6166771-6166790 Msa0099830:CDS 50.0%
TGAAGGAGTTTGGAGAAGCC+AGG + chr1_3:6166859-6166878 Msa0099840:five_prime_UTR 50.0%
AAGAAGCAGAAGCTGCTTCC+TGG - chr1_3:6166880-6166899 Msa0099830:CDS 50.0%
AGCAGCTTCTGCTTCTTCGT+TGG + chr1_3:6166883-6166902 Msa0099840:five_prime_UTR 50.0%
AGCTTCTGCTTCTTCGTTGG+TGG + chr1_3:6166886-6166905 Msa0099840:five_prime_UTR 50.0%
! GTTGGCTGTTGTTGTTGTGG+TGG + chr1_3:6166942-6166961 Msa0099840:five_prime_UTR 50.0%
!! GTTGTTGTTGTGGTGGTGGA+AGG + chr1_3:6166949-6166968 Msa0099840:five_prime_UTR 50.0%
GATACATAGAGCAGCTGCGT+CGG + chr1_3:6167039-6167058 Msa0099840:five_prime_UTR 50.0%
CCAAGTCTGTGAACAAGCTC+CGG - chr1_3:6167083-6167102 Msa0099830:CDS 50.0%
! TTGGGAGTGTAGAAGAGGAG+AGG + chr1_3:6167333-6167352 Msa0099840:five_prime_UTR 50.0%
GAGTGTAGAAGAGGAGAGGA+GGG + chr1_3:6167337-6167356 Msa0099840:five_prime_UTR 50.0%
CTAATTGAGGGATGACCCGT+GGG + chr1_3:6167428-6167447 Msa0099840:five_prime_UTR 50.0%
AGGGATGCAAGGACAGAAAG+AGG + chr1_3:6167491-6167510 Msa0099840:five_prime_UTR 50.0%
GGGATGCAAGGACAGAAAGA+GGG + chr1_3:6167492-6167511 Msa0099840:five_prime_UTR 50.0%
!! AGGGACTCTTCTGAGATGAG+TGG + chr1_3:6167511-6167530 Msa0099840:five_prime_UTR 50.0%
GCATCAATGTGGCATACTGG+TGG + chr1_3:6167741-6167760 Msa0099840:five_prime_UTR 50.0%
AGCGGGTAAAGCGAAATCCA+AGG - chr1_3:6167775-6167794 None:intergenic 50.0%
AGCGCAATGCAACTCACGAA+AGG + chr1_3:6168010-6168029 Msa0099840:CDS 50.0%
ACGTTTCACCTCAACTTCGC+AGG - chr1_3:6168054-6168073 None:intergenic 50.0%
TGCGAAGTTGAGGTGAAACG+TGG + chr1_3:6168053-6168072 Msa0099840:CDS 50.0%
TTCGTGAAGCAGGGGAACAA+TGG + chr1_3:6168216-6168235 Msa0099840:CDS 50.0%
GAACTCTCTCAACATGCACC+TGG + chr1_3:6168260-6168279 Msa0099840:CDS 50.0%
!! AGAAAACGCACTTTGGTGCC+AGG - chr1_3:6168281-6168300 None:intergenic 50.0%
! GTAACCACATGTGTCGGTGT+CGG + chr1_3:6168654-6168673 Msa0099840:intron 50.0%
!! TCGTGGTGGATCTCACACTT+TGG + chr1_3:6168894-6168913 Msa0099840:intron 50.0%
TGTTTCCTTTCTTCCAGCCG+AGG + chr1_3:6169702-6169721 Msa0099840:intron 50.0%
GGCGGTGGAAAGAGAAGCTA+GGG - chr1_3:6166370-6166389 Msa0099830:CDS 55.0%
TCTCTTTCCACCGCCGTCAT+CGG + chr1_3:6166375-6166394 Msa0099840:five_prime_UTR 55.0%
TCCGTCATCACGTTTCCGTC+TGG + chr1_3:6166459-6166478 Msa0099840:five_prime_UTR 55.0%
GTGTTCGTGTTCGTGTTCGG+TGG + chr1_3:6166682-6166701 Msa0099840:five_prime_UTR 55.0%
! TTCGTGTTCGTGTTCGGTGG+TGG + chr1_3:6166685-6166704 Msa0099840:five_prime_UTR 55.0%
! GGCTGTTGTTGTTGTGGTGG+TGG + chr1_3:6166945-6166964 Msa0099840:five_prime_UTR 55.0%
!! GTTGTTGTGGTGGTGGAAGG+TGG + chr1_3:6166952-6166971 Msa0099840:five_prime_UTR 55.0%
!! GAGCTAGTGGGTTAGCAGAG+TGG + chr1_3:6166999-6167018 Msa0099840:five_prime_UTR 55.0%
! CCGGAGCTTGTTCACAGACT+TGG + chr1_3:6167080-6167099 Msa0099840:five_prime_UTR 55.0%
AGACTTGGCAGAGTGTGACG+CGG + chr1_3:6167095-6167114 Msa0099840:five_prime_UTR 55.0%
!! AGGCGAGTTTGTTGGCAGCA+TGG + chr1_3:6167128-6167147 Msa0099840:five_prime_UTR 55.0%
GGAGTGTAGAAGAGGAGAGG+AGG + chr1_3:6167336-6167355 Msa0099840:five_prime_UTR 55.0%
AGAGGAGAGGAGGGAATGTG+TGG + chr1_3:6167346-6167365 Msa0099840:five_prime_UTR 55.0%
GAGGAGGGAATGTGTGGTTG+TGG + chr1_3:6167352-6167371 Msa0099840:five_prime_UTR 55.0%
GCTAATTGAGGGATGACCCG+TGG + chr1_3:6167427-6167446 Msa0099840:five_prime_UTR 55.0%
TTTGGCTAAGGCTCACCCAC+GGG - chr1_3:6167446-6167465 None:intergenic 55.0%
CTTTGGCTAAGGCTCACCCA+CGG - chr1_3:6167447-6167466 None:intergenic 55.0%
AAACCCTGCCTGCGAAGTTG+AGG + chr1_3:6168043-6168062 Msa0099840:CDS 55.0%
TCACCTCAACTTCGCAGGCA+GGG - chr1_3:6168049-6168068 None:intergenic 55.0%
TTCACCTCAACTTCGCAGGC+AGG - chr1_3:6168050-6168069 None:intergenic 55.0%
ATGTCCGACACCGACACATG+TGG - chr1_3:6168661-6168680 None:intergenic 55.0%
GCTTTCCTCGGCTGGAAGAA+AGG - chr1_3:6169710-6169729 None:intergenic 55.0%
CGGCGGTGGAAAGAGAAGCT+AGG - chr1_3:6166371-6166390 Msa0099830:CDS 60.0%
ATCGGAACCGCCACCGTCAT+CGG + chr1_3:6166393-6166412 Msa0099840:five_prime_UTR 60.0%
CCAAACGACGGCGACAGAGA+CGG - chr1_3:6166421-6166440 Msa0099830:CDS 60.0%
TCTTCGCCGTCGATGGAGGT+TGG - chr1_3:6166492-6166511 Msa0099830:CDS 60.0%
GGTATCTTCGCCGTCGATGG+AGG - chr1_3:6166496-6166515 Msa0099830:intron 60.0%
! GTGCCGGGACTGTTTTGCTC+CGG + chr1_3:6166749-6166768 Msa0099840:five_prime_UTR 60.0%
ACGCCGGAGCAAAACAGTCC+CGG - chr1_3:6166755-6166774 Msa0099830:CDS 60.0%
!! TGGTGGTGGAAGGTGGTGAG+AGG + chr1_3:6166959-6166978 Msa0099840:five_prime_UTR 60.0%
!! GGTGGTGGAAGGTGGTGAGA+GGG + chr1_3:6166960-6166979 Msa0099840:five_prime_UTR 60.0%
TGGTGAGAGGGACACGTTCG+TGG + chr1_3:6166972-6166991 Msa0099840:five_prime_UTR 60.0%
TGAGAGGGACACGTTCGTGG+CGG + chr1_3:6166975-6166994 Msa0099840:five_prime_UTR 60.0%
GCTTTGCAGGTGACGTGAGC+CGG + chr1_3:6167061-6167080 Msa0099840:five_prime_UTR 60.0%
CTTCCAGCCGAGGAAAGCAG+AGG + chr1_3:6169712-6169731 Msa0099840:intron 60.0%
TCTCCTCTGCTTTCCTCGGC+TGG - chr1_3:6169718-6169737 None:intergenic 60.0%
! GACGGTGGCGGTTCCGATGA+CGG - chr1_3:6166391-6166410 Msa0099830:CDS 65.0%
!! GACAGAGACGGCGCCGATGA+CGG - chr1_3:6166409-6166428 Msa0099830:CDS 65.0%
CCGTCTCTGTCGCCGTCGTT+TGG + chr1_3:6166418-6166437 Msa0099840:five_prime_UTR 65.0%
CACCGGAACGACGCCATCTG+CGG + chr1_3:6166724-6166743 Msa0099840:five_prime_UTR 65.0%
CACCGCAGATGGCGTCGTTC+CGG - chr1_3:6166729-6166748 Msa0099830:CDS 65.0%
GTTCGTGGCGGCGAGCTAGT+GGG + chr1_3:6166987-6167006 Msa0099840:five_prime_UTR 65.0%
!! AGCAGCTGCGTCGGCTTTGC+AGG + chr1_3:6167048-6167067 Msa0099840:five_prime_UTR 65.0%
CTTGGCAGAGTGTGACGCGG+TGG + chr1_3:6167098-6167117 Msa0099840:five_prime_UTR 65.0%
! AGAGTGTGACGCGGTGGTGG+CGG + chr1_3:6167104-6167123 Msa0099840:five_prime_UTR 65.0%
! GTGGCGGGAGGCGAGTTTGT+TGG + chr1_3:6167120-6167139 Msa0099840:five_prime_UTR 65.0%
! GGTGGCGGTTCCGATGACGG+CGG - chr1_3:6166388-6166407 Msa0099830:CDS 70.0%
!! AGAGACGGCGCCGATGACGG+TGG - chr1_3:6166406-6166425 Msa0099830:CDS 70.0%
CGGAACGACGCCATCTGCGG+TGG + chr1_3:6166727-6166746 Msa0099840:five_prime_UTR 70.0%
GACGCCATCTGCGGTGGTGC+CGG + chr1_3:6166733-6166752 Msa0099840:five_prime_UTR 70.0%
ACGCCATCTGCGGTGGTGCC+GGG + chr1_3:6166734-6166753 Msa0099840:five_prime_UTR 70.0%
AGTCCCGGCACCACCGCAGA+TGG - chr1_3:6166740-6166759 Msa0099830:CDS 70.0%
CGTTCGTGGCGGCGAGCTAG+TGG + chr1_3:6166986-6167005 Msa0099840:five_prime_UTR 70.0%
GGCAGAGTGTGACGCGGTGG+TGG + chr1_3:6167101-6167120 Msa0099840:five_prime_UTR 70.0%
! GAGTGTGACGCGGTGGTGGC+GGG + chr1_3:6167105-6167124 Msa0099840:five_prime_UTR 70.0%
! GGCGGTTCCGATGACGGCGG+TGG - chr1_3:6166385-6166404 Msa0099830:CDS 75.0%
!! GACGGCGCCGATGACGGTGG+CGG - chr1_3:6166403-6166422 Msa0099830:CDS 75.0%
!! TGTGACGCGGTGGTGGCGGG+AGG + chr1_3:6167108-6167127 Msa0099840:five_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 6165854 6170541 6165854 ID=Msa0099840;Name=Msa0099840
chr1_3 mRNA 6165854 6170541 6165854 ID=Msa0099840-mRNA-1;Parent=Msa0099840;Name=Msa0099840-mRNA-1;_AED=0.00;_eAED=0.00;_QI=2079|1|1|1|1|1|2|793|128
chr1_3 exon 6165854 6168289 6165854 ID=Msa0099840-mRNA-1:exon:2311;Parent=Msa0099840-mRNA-1
chr1_3 exon 6169719 6170541 6169719 ID=Msa0099840-mRNA-1:exon:2312;Parent=Msa0099840-mRNA-1
chr1_3 five_prime_UTR 6165854 6167932 6165854 ID=Msa0099840-mRNA-1:five_prime_utr;Parent=Msa0099840-mRNA-1
chr1_3 CDS 6167933 6168289 6167933 ID=Msa0099840-mRNA-1:cds;Parent=Msa0099840-mRNA-1
chr1_3 CDS 6169719 6169748 6169719 ID=Msa0099840-mRNA-1:cds;Parent=Msa0099840-mRNA-1
chr1_3 three_prime_UTR 6169749 6170541 6169749 ID=Msa0099840-mRNA-1:three_prime_utr;Parent=Msa0099840-mRNA-1
Gene Sequence

>Msa0099840

ATGCTGAAATTCCTATCGAGAGTGAAGGTTGAGTTCAATGCGCTAGATCCACGAACTGCATCATGTATGGAGTTTCTAGCGCAATGCAACTCACGAAAGGCGAAAGAATCAAACCCTGCCTGCGAAGTTGAGGTGAAACGTGGAAATGTGGAACACCCACCACAGATCACAGTGACATTTGTTAACGGTGTGGAAGAAGCTTTCGATGCAACATCAACGCCGGTTCACCGTATAACGAAGATGATTCTGGAAAAGGGTCAGCTCCTTGAGACTGAACAAATGTTTCGTGAAGCAGGGGAACAATGGCCTGTTATCATTCCTGATGAAGAACTCTCTCAACATGCACCTGGCACCAAACCGAGGAAAGCAGAGGAGAAGAAGCAATAG
Protein sequence

>Msa0099840

MLKFLSRVKVEFNALDPRTASCMEFLAQCNSRKAKESNPACEVEVKRGNVEHPPQITVTFVNGVEEAFDATSTPVHRITKMILEKGQLLETEQMFREAGEQWPVIIPDEELSQHAPGTKPRKAEEKKQ*