Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0101740 | sp|Q8RYD1|REM16_ARATH | 47.651 | 149 | 72 | 4 | 5 | 149 | 3 | 149 | 3.23e-35 | 140 |
| Msa0101740 | sp|Q8RYD1|REM16_ARATH | 32.423 | 293 | 139 | 6 | 187 | 422 | 11 | 301 | 8.20e-31 | 127 |
| Msa0101740 | sp|Q8RYD1|REM16_ARATH | 44.295 | 149 | 65 | 4 | 334 | 466 | 11 | 157 | 6.50e-30 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0101740 | G7I4K4 | 59.167 | 360 | 72 | 6 | 9 | 332 | 6 | 326 | 3.47e-122 | 402 |
| Msa0101740 | G7I4K4 | 65.714 | 140 | 44 | 2 | 3 | 139 | 159 | 297 | 1.73e-45 | 188 |
| Msa0101740 | G7I4K4 | 88.639 | 845 | 39 | 8 | 177 | 967 | 1 | 842 | 0.0 | 1483 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0101740 | Msa0145560 | 0.891135 | 5.235420e-74 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0101740 | MtrunA17_Chr1g0154101 | 88.639 | 845 | 39 | 8 | 177 | 967 | 1 | 842 | 0.0 | 1483 |
| Msa0101740 | MtrunA17_Chr1g0154101 | 59.167 | 360 | 72 | 6 | 9 | 332 | 6 | 326 | 6.68e-126 | 402 |
| Msa0101740 | MtrunA17_Chr1g0154101 | 65.714 | 140 | 44 | 2 | 3 | 139 | 159 | 297 | 3.32e-49 | 188 |
| Msa0101740 | MtrunA17_Chr1g0154131 | 56.757 | 592 | 102 | 11 | 177 | 734 | 1 | 472 | 0.0 | 611 |
| Msa0101740 | MtrunA17_Chr1g0154131 | 46.346 | 520 | 162 | 10 | 1 | 447 | 1 | 476 | 1.51e-128 | 397 |
| Msa0101740 | MtrunA17_Chr1g0154131 | 31.915 | 282 | 166 | 5 | 674 | 954 | 216 | 472 | 5.18e-34 | 137 |
| Msa0101740 | MtrunA17_Chr1g0154071 | 55.838 | 591 | 117 | 10 | 182 | 736 | 9 | 491 | 0.0 | 567 |
| Msa0101740 | MtrunA17_Chr1g0154071 | 67.147 | 347 | 47 | 5 | 1 | 332 | 1 | 295 | 1.41e-147 | 447 |
| Msa0101740 | MtrunA17_Chr1g0154071 | 60.714 | 140 | 42 | 2 | 323 | 449 | 3 | 142 | 3.51e-44 | 167 |
| Msa0101740 | MtrunA17_Chr1g0154071 | 30.571 | 350 | 197 | 12 | 641 | 956 | 154 | 491 | 1.76e-32 | 132 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0101740 | AT4G33280.1 | 47.651 | 149 | 72 | 4 | 5 | 149 | 3 | 149 | 3.28e-36 | 140 |
| Msa0101740 | AT4G33280.1 | 32.423 | 293 | 139 | 6 | 187 | 422 | 11 | 301 | 8.34e-32 | 127 |
| Msa0101740 | AT4G33280.1 | 44.295 | 149 | 65 | 4 | 334 | 466 | 11 | 157 | 6.61e-31 | 125 |
| Msa0101740 | AT4G33280.2 | 48.276 | 145 | 68 | 5 | 5 | 145 | 3 | 144 | 4.10e-36 | 139 |
| Msa0101740 | AT4G33280.2 | 50.000 | 130 | 50 | 3 | 187 | 303 | 11 | 138 | 8.30e-32 | 126 |
| Msa0101740 | AT4G33280.2 | 39.264 | 163 | 79 | 3 | 334 | 476 | 11 | 173 | 2.41e-31 | 125 |
| Msa0101740 | AT4G33280.3 | 48.276 | 145 | 68 | 5 | 5 | 145 | 3 | 144 | 2.78e-35 | 139 |
| Msa0101740 | AT4G33280.3 | 50.000 | 130 | 50 | 3 | 187 | 303 | 11 | 138 | 7.53e-31 | 126 |
| Msa0101740 | AT4G33280.3 | 39.264 | 163 | 79 | 3 | 334 | 476 | 11 | 173 | 2.71e-30 | 124 |
| Msa0101740 | AT3G06220.1 | 31.731 | 104 | 67 | 1 | 215 | 314 | 23 | 126 | 7.57e-16 | 77.0 |
Find 211 sgRNAs with CRISPR-Local
Find 1077 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGAGGAGGTAAATGATT+GGG | 0.509472 | 1_3:+8379874 | None:intergenic |
| AAAAGATGGGTTAGACTCCC+AGG | 0.502465 | 1_3:-8378867 | Msa0101740:intron |
| AAAATCTTTGGGAAGAAGTT+GGG | 0.404051 | 1_3:+8383383 | None:intergenic |
| AAAATTGGTGTGAAGCAATA+GGG | 0.495077 | 1_3:+8391384 | None:intergenic |
| AAACAAAGACAGCTGTACCG+AGG | 0.720792 | 1_3:-8378336 | Msa0101740:intron |
| AACAAAATATGGAAAAGAGG+AGG | 0.585641 | 1_3:+8379862 | None:intergenic |
| AACAAGTTGTCGTTCAAAAT+TGG | 0.231202 | 1_3:+8391369 | None:intergenic |
| AACACCACCGGGACCTTTAA+GGG | 0.419570 | 1_3:+8382375 | None:intergenic |
| AACTCCACTAGGACCTTTAA+GGG | 0.479312 | 1_3:+8383764 | None:intergenic |
| AACTCCGTCCCATATGTTCA+AGG | 0.509291 | 1_3:-8377970 | Msa0101740:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATGATTTCCTCCAATGATT+TGG | + | chr1_3:8377461-8377480 | None:intergenic | 30.0% | |
| CATTGGAGGAAATCATTTAT+TGG | - | chr1_3:8377464-8377483 | Msa0101740:three_prime_UTR | 30.0% | |
| GAAATGGATGAACTGAAAAT+GGG | + | chr1_3:8377491-8377510 | None:intergenic | 30.0% | |
| TGAAATGGATGAACTGAAAA+TGG | + | chr1_3:8377492-8377511 | None:intergenic | 30.0% | |
| AAAAAATTCCCTCAAATTGG+AGG | + | chr1_3:8377971-8377990 | None:intergenic | 30.0% | |
| GATACTATTTACTTCACGAA+CGG | - | chr1_3:8378378-8378397 | Msa0101740:intron | 30.0% | |
| CACCATTGTACTTAAATACA+AGG | + | chr1_3:8378449-8378468 | None:intergenic | 30.0% | |
| ACAGTTCACCTGAAAAATTA+TGG | - | chr1_3:8378583-8378602 | Msa0101740:intron | 30.0% | |
| CAGTTCACCTGAAAAATTAT+GGG | - | chr1_3:8378584-8378603 | Msa0101740:intron | 30.0% | |
| AAAATTGGTGTGAAGCAATA+GGG | + | chr1_3:8378652-8378671 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_3 | gene | 8377427 | 8392628 | 8377427 | ID=Msa0101740;Name=Msa0101740 |
| chr1_3 | mRNA | 8377427 | 8392628 | 8377427 | ID=Msa0101740-mRNA-1;Parent=Msa0101740;Name=Msa0101740-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|0.35|0.2|1|0.5|0.4|15|362|967 |
| chr1_3 | exon | 8377427 | 8378082 | 8377427 | ID=Msa0101740-mRNA-1:exon:3359;Parent=Msa0101740-mRNA-1 |
| chr1_3 | exon | 8378177 | 8378350 | 8378177 | ID=Msa0101740-mRNA-1:exon:3358;Parent=Msa0101740-mRNA-1 |
| chr1_3 | exon | 8378610 | 8378828 | 8378610 | ID=Msa0101740-mRNA-1:exon:3357;Parent=Msa0101740-mRNA-1 |
| chr1_3 | exon | 8378868 | 8379107 | 8378868 | ID=Msa0101740-mRNA-1:exon:3356;Parent=Msa0101740-mRNA-1 |
| chr1_3 | exon | 8379820 | 8379995 | 8379820 | ID=Msa0101740-mRNA-1:exon:3355;Parent=Msa0101740-mRNA-1 |
| chr1_3 | exon | 8381579 | 8381737 | 8381579 | ID=Msa0101740-mRNA-1:exon:3354;Parent=Msa0101740-mRNA-1 |
| chr1_3 | exon | 8381819 | 8382043 | 8381819 | ID=Msa0101740-mRNA-1:exon:3353;Parent=Msa0101740-mRNA-1 |
| chr1_3 | exon | 8382080 | 8382220 | 8382080 | ID=Msa0101740-mRNA-1:exon:3352;Parent=Msa0101740-mRNA-1 |
| chr1_3 | exon | 8382257 | 8382452 | 8382257 | ID=Msa0101740-mRNA-1:exon:3351;Parent=Msa0101740-mRNA-1 |
| chr1_3 | exon | 8383371 | 8383592 | 8383371 | ID=Msa0101740-mRNA-1:exon:3350;Parent=Msa0101740-mRNA-1 |
| chr1_3 | exon | 8383629 | 8383841 | 8383629 | ID=Msa0101740-mRNA-1:exon:3349;Parent=Msa0101740-mRNA-1 |
| chr1_3 | exon | 8385183 | 8385299 | 8385183 | ID=Msa0101740-mRNA-1:exon:3348;Parent=Msa0101740-mRNA-1 |
| chr1_3 | exon | 8391372 | 8391596 | 8391372 | ID=Msa0101740-mRNA-1:exon:3347;Parent=Msa0101740-mRNA-1 |
| chr1_3 | exon | 8391606 | 8391785 | 8391606 | ID=Msa0101740-mRNA-1:exon:3346;Parent=Msa0101740-mRNA-1 |
| chr1_3 | exon | 8392506 | 8392628 | 8392506 | ID=Msa0101740-mRNA-1:exon:3345;Parent=Msa0101740-mRNA-1 |
| chr1_3 | CDS | 8392506 | 8392628 | 8392506 | ID=Msa0101740-mRNA-1:cds;Parent=Msa0101740-mRNA-1 |
| chr1_3 | CDS | 8391606 | 8391785 | 8391606 | ID=Msa0101740-mRNA-1:cds;Parent=Msa0101740-mRNA-1 |
| chr1_3 | CDS | 8391372 | 8391596 | 8391372 | ID=Msa0101740-mRNA-1:cds;Parent=Msa0101740-mRNA-1 |
| chr1_3 | CDS | 8385183 | 8385299 | 8385183 | ID=Msa0101740-mRNA-1:cds;Parent=Msa0101740-mRNA-1 |
| chr1_3 | CDS | 8383629 | 8383841 | 8383629 | ID=Msa0101740-mRNA-1:cds;Parent=Msa0101740-mRNA-1 |
| chr1_3 | CDS | 8383371 | 8383592 | 8383371 | ID=Msa0101740-mRNA-1:cds;Parent=Msa0101740-mRNA-1 |
| chr1_3 | CDS | 8382257 | 8382452 | 8382257 | ID=Msa0101740-mRNA-1:cds;Parent=Msa0101740-mRNA-1 |
| chr1_3 | CDS | 8382080 | 8382220 | 8382080 | ID=Msa0101740-mRNA-1:cds;Parent=Msa0101740-mRNA-1 |
| chr1_3 | CDS | 8381819 | 8382043 | 8381819 | ID=Msa0101740-mRNA-1:cds;Parent=Msa0101740-mRNA-1 |
| chr1_3 | CDS | 8381579 | 8381737 | 8381579 | ID=Msa0101740-mRNA-1:cds;Parent=Msa0101740-mRNA-1 |
| chr1_3 | CDS | 8379820 | 8379995 | 8379820 | ID=Msa0101740-mRNA-1:cds;Parent=Msa0101740-mRNA-1 |
| chr1_3 | CDS | 8378868 | 8379107 | 8378868 | ID=Msa0101740-mRNA-1:cds;Parent=Msa0101740-mRNA-1 |
| chr1_3 | CDS | 8378610 | 8378828 | 8378610 | ID=Msa0101740-mRNA-1:cds;Parent=Msa0101740-mRNA-1 |
| chr1_3 | CDS | 8378177 | 8378350 | 8378177 | ID=Msa0101740-mRNA-1:cds;Parent=Msa0101740-mRNA-1 |
| chr1_3 | CDS | 8377789 | 8378082 | 8377789 | ID=Msa0101740-mRNA-1:cds;Parent=Msa0101740-mRNA-1 |
| chr1_3 | three_prime_UTR | 8377427 | 8377788 | 8377427 | ID=Msa0101740-mRNA-1:three_prime_utr;Parent=Msa0101740-mRNA-1 |
| Gene Sequence |
>Msa0101740 ATGGAAGGAACTCAAAACTGTGTTGGAAGCACCAAATCATTGGAGGAAATCATTTATTGGACCCATTTTCAGTTCATCCATTTCACCCTTTTTCTGAGCATCACCCATTTTCACCATCAACTTGCACTTCCGAAAACATTTTCAGACAATTTAAAGAAGAAGTTGCCAGCAAATGTGACACTTAAAGGTCCTAGCGGAATTGTGTGGAATATAGGGCTTACAACTAGAGGTGATACTATTTACTTCACGAACGGTTGGCAAAAATTCGTGAAAGACCACTCCTTGAAGCAGAATGATTTCCTTTACAATGGTGAATCACAGTTTGAGGTTTTAATCTTTAATGGAAATACTTTCTGTGAGAAGGCAGCGTCATATTTTGTTCAAAAATGTGGACAAGCTCATAGTCCTTCCAGTGGTGGTGTTGAATACAGTTCACCTGAAAAATTATGGGCGGAAGACATTTACTGGAGCTATTTCAAATTCACACATTTCACCCTATTGCTTCACACCAATTTTGAACGACAACTTATGGGAGGAGCAGGTGGAAGCAGCAAATCTTGGGAGAAAGAGATTTACTGGACCCATTTTCAGTTCATTCATTTCATCCAATTTCTTCAAACCACCACCGATTTTCAACAACAACTTGCCCTTCCCAAAACATTTTCAGACAATTTAAAGAAGAAGTTGCCAGACAATGTGACCCTTAAAGGTCCTAGTGGAGTTGTGTGGAATGTCGGGCTGACAGCTAGAGATGATACCGTTTACTTCACAAATGGATGGCAAAGATTCGTGAAAGATCACTCTTTGAAAGAGAATGATTTCCTTGTCTTTAAGTACAATGGGGAATCACTCTTTGAAGCAGCTTCTTATTTTGTTGGAAAATGTGGACAAGCTCATACTGAACAAGGGTGTAACAAGGGAAAAAGCTCCAATAATTCTGTTGAAGAAGTCAGTACTCCTTCTAATGGCGGTGTTGAATGCAGTTCGCCTGAAAAATCATGGGAGGAAGACATTTACTGGACCCATTTTCAGTTCATCCATTTCACCCAACTTCTTCCCAAAGATTTTAAGCAACAACTTGCACTTCCTAAAACATTTTCAGACAATTTAAATAAGAAGTTGCCAGAAAATGTGACCCTTAAAGGTCCCGGTGGTGTTGCATGGAAAATAGGGCTCATAACTAGAGACGATACCGTTTACTTCGTGGACGGTTGGCAACGATTCGTGAACGATCACTCTTTGAAAGATAATGATTTCCTTGTCTTTAATTACAATGAAAGCTTCTGTGAGAAAGAAGCTTCTTATTTTGTCGGAAAATGCGGCCATACTCAAACTGAACTAGGGGGTAGCAAAGCAAAGGAAACAAACAATTCTATTGAAGAAGTCAATACTGCTTCTAATGGTGGTGTTGAATGTGGAACACCATTAGCTGTACCTGTTGAAACTACCAATGAAAAGACTTTTAATGCTGGTGTCAAATCTGATTCCCCTGAGCAGTTCATAGCTGATACAGTCACTAAGACCACAGCTATACAGTTTCCCTACCAACCAACCGGAAAAAGGAGCAAGAGAAGATCATCGACATCAGCTAATTGTTGTTGGAGAGCACTTGACTGGGTGGCTTATAACAAGCAACACTCAGTCACTATAGCGGTTCCCTCCCATACAACCAACCAAAAGACCAAGAAGTCTGTTAATGAAGTTACGCCTGGCCGGAGTAAGAAGCGGGGAAGACAAGCAAAAACAGCTACTTCTGGCGAGAGAGCAATTGATTGGGTGGCTTGTAACAAGGAAAACTCAGAGGCAGCCATCCTGAATAGAAGTGGGAAGGCAGATGGCCATGATATTCTCAGTGGAGCTTCACTGTCTAAGTTATCACCACATGATGAGAAAGAGATAGCCCAATCATTTACCTCCTCTTTTCCATATTTTGTTAAAATAATGAAAACGTTCAATGTTGAACGTCCACGTATTCTGGATCATCTTCCAAACTGCAAGATAAAGATTATTCTTCATAATTTGAAGGGAGAACAGTGGACCGTCAATTCTGTTCCCAGAACTAGATTGCATGCAAGTCACATTCTATGCGGAGGATGGTTGAATTTTGTTCGTGGCAATAGCCTAAAGATTGGAGATGTCTGTATTTTCGAACTTATAGGTGAGTGTGAATTACGTGTTCGCATCAATGAGGTTGGAAAAGATGGGTTAGACTCCCAGGAAGTCGCTAGCCATAAGACTTCCAGATTTATGCCGAAGAAACATAAAATCAGCTCAAAGTGCAGTGCGGAAGTTGATCTATTAGATAAAAAGTTGTCAGAGATATGTCAAGAAGCTGCTTTATCCTCTGAGTTAAAGAAATCCGGTAGAGCTTCAAATACTTCGAAGAAGGCAGGGCTTTCCCCCAAGTCAAAAGCTGCCCATAAAAAGTCGGGTATGACAGCTGTACCGAGGAAACACCATGTTGATGATGGACTAAGCTCCCAGGCTAATGATAGTTTGAAAATGTTGTTTGCACTCGACGAACAAAGAGTAGCTGAAGCTTTCACTTCCCCATTTCCATATTTTGTGAAAATCATGAAGAAGTTCAATGTCTGTGGATCATACACTTTGAAAATCCCATGCCAGTTCTCTACTGCACATCTCCCTGCTTGCAATACAGAAGTTACGCTTCGCAATTCAAGAGGCAAATGTTGGACTGTGAACTCCGTCCCATATGTTCAAGGGGAAACAACTATTCACACTTTTTGTGGCGGATGGAATGCCTTTGTTCGTGATAATGACATCAATTTTGGTGACACTTGTATTTTTGAGCTCGTTTCTGATTATGTGATGCACGTTCGTATATCTGGGGTTGGAAAGGAAGGTCTTGATCATCAAAATGGGAATGTGAAACTTAATAGCTAG |
| Protein sequence |
>Msa0101740 MEGTQNCVGSTKSLEEIIYWTHFQFIHFTLFLSITHFHHQLALPKTFSDNLKKKLPANVTLKGPSGIVWNIGLTTRGDTIYFTNGWQKFVKDHSLKQNDFLYNGESQFEVLIFNGNTFCEKAASYFVQKCGQAHSPSSGGVEYSSPEKLWAEDIYWSYFKFTHFTLLLHTNFERQLMGGAGGSSKSWEKEIYWTHFQFIHFIQFLQTTTDFQQQLALPKTFSDNLKKKLPDNVTLKGPSGVVWNVGLTARDDTVYFTNGWQRFVKDHSLKENDFLVFKYNGESLFEAASYFVGKCGQAHTEQGCNKGKSSNNSVEEVSTPSNGGVECSSPEKSWEEDIYWTHFQFIHFTQLLPKDFKQQLALPKTFSDNLNKKLPENVTLKGPGGVAWKIGLITRDDTVYFVDGWQRFVNDHSLKDNDFLVFNYNESFCEKEASYFVGKCGHTQTELGGSKAKETNNSIEEVNTASNGGVECGTPLAVPVETTNEKTFNAGVKSDSPEQFIADTVTKTTAIQFPYQPTGKRSKRRSSTSANCCWRALDWVAYNKQHSVTIAVPSHTTNQKTKKSVNEVTPGRSKKRGRQAKTATSGERAIDWVACNKENSEAAILNRSGKADGHDILSGASLSKLSPHDEKEIAQSFTSSFPYFVKIMKTFNVERPRILDHLPNCKIKIILHNLKGEQWTVNSVPRTRLHASHILCGGWLNFVRGNSLKIGDVCIFELIGECELRVRINEVGKDGLDSQEVASHKTSRFMPKKHKISSKCSAEVDLLDKKLSEICQEAALSSELKKSGRASNTSKKAGLSPKSKAAHKKSGMTAVPRKHHVDDGLSSQANDSLKMLFALDEQRVAEAFTSPFPYFVKIMKKFNVCGSYTLKIPCQFSTAHLPACNTEVTLRNSRGKCWTVNSVPYVQGETTIHTFCGGWNAFVRDNDINFGDTCIFELVSDYVMHVRISGVGKEGLDHQNGNVKLNS* |
