AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0102160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0102160 MtrunA17_Chr1g0156031 98.529 340 4 1 1 339 1 340 0.0 699
Msa0102160 MtrunA17_Chr8g0385961 49.713 348 127 10 2 339 3 312 6.53e-104 307
Msa0102160 MtrunA17_Chr6g0479911 41.040 173 102 0 167 339 117 289 1.27e-45 157
Msa0102160 MtrunA17_Chr8g0375531 51.613 124 60 0 216 339 157 280 2.37e-42 148
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0102160 AT5G42520.1 65.084 358 90 15 1 339 1 342 1.17e-149 425
Msa0102160 AT5G42520.2 63.966 358 90 15 1 339 1 338 1.96e-145 414
Msa0102160 AT5G42520.3 62.543 291 84 11 65 339 1 282 2.21e-116 338
Msa0102160 AT2G21240.1 48.830 342 115 7 5 339 8 296 6.54e-100 297
Msa0102160 AT2G21240.2 48.830 342 115 7 5 339 8 296 6.54e-100 297
Msa0102160 AT4G38910.2 45.266 338 120 7 5 339 8 283 8.46e-93 278
Msa0102160 AT4G38910.1 45.253 316 111 6 24 339 5 258 1.35e-86 261
Msa0102160 AT2G35550.2 55.932 118 52 0 222 339 109 226 1.46e-45 155
Msa0102160 AT2G35550.4 55.932 118 52 0 222 339 109 226 1.46e-45 155
Msa0102160 AT2G35550.3 55.932 118 52 0 222 339 109 226 1.46e-45 155
Msa0102160 AT2G35550.1 55.932 118 52 0 222 339 154 271 1.91e-45 156
Msa0102160 AT1G68120.1 56.075 107 47 0 233 339 163 269 1.36e-43 151
Msa0102160 AT2G01930.2 55.455 110 49 0 230 339 174 283 2.13e-43 151
Msa0102160 AT2G01930.1 55.455 110 49 0 230 339 174 283 2.13e-43 151
Msa0102160 AT1G14685.1 54.545 110 50 0 230 339 170 279 2.34e-42 148
Msa0102160 AT1G14685.4 54.545 110 50 0 230 339 170 279 2.34e-42 148
Msa0102160 AT1G14685.3 54.545 110 50 0 230 339 170 279 2.34e-42 148
Msa0102160 AT1G14685.2 54.545 110 50 0 230 339 170 279 2.34e-42 148

Find 118 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTGGCCCAATGGTCTTTA+AGG 0.149233 1_3:+8882178 None:intergenic
GGTAAACCATTTGCCTAATA+TGG 0.151975 1_3:-8882769 Msa0102160:CDS
TTTCCTTGGATCCTAATTAT+AGG 0.234185 1_3:+8881997 None:intergenic
CCATTCAAGCTCTTTGTTGT+TGG 0.281988 1_3:+8882551 None:intergenic
TTGCTGTACAACCACTTTAT+CGG 0.289810 1_3:-8882331 Msa0102160:CDS
TCTGGCCCTCTCTGAGAAAA+AGG 0.293315 1_3:-8882994 Msa0102160:CDS
AGTAGTGAGGAAATGATAAA+TGG 0.302982 1_3:-8882525 Msa0102160:CDS
GATTGACATAATTTGCTTCA+TGG 0.306231 1_3:+8883052 None:intergenic
GTTGAACAAGGAGGAAAATA+AGG 0.306983 1_3:-8882061 Msa0102160:three_prime_UTR
CTGATGGACGATCGTGAAAA+TGG 0.307285 1_3:-8883274 Msa0102160:CDS
TGGTGGCAATCGCATTTAAT+AGG 0.330547 1_3:-8881916 Msa0102160:three_prime_UTR
TAAGAAGAATCACCCATATT+AGG 0.336214 1_3:+8882756 None:intergenic
AATGTGTCATTCCTATAATT+AGG 0.341337 1_3:-8882008 Msa0102160:three_prime_UTR
CTTACTTTCCTTTGGCCGTT+TGG 0.363106 1_3:+8882653 None:intergenic
ACCGAGTTGGATGCGGGATT+AGG 0.375501 1_3:-8883341 Msa0102160:five_prime_UTR
CAATGGTCTTTAAGGTCAAC+CGG 0.380775 1_3:+8882186 None:intergenic
TTTCCCAACCTCTGAAGAAA+TGG 0.384675 1_3:+8882689 None:intergenic
GAATCAGACTTACTTTCCTT+TGG 0.396516 1_3:+8882645 None:intergenic
CCAACAACAAAGAGCTTGAA+TGG 0.401945 1_3:-8882551 Msa0102160:CDS
CTACCATTTGTTAATTCTGA+TGG 0.405480 1_3:-8883290 Msa0102160:exon
GGCCAGATTTCTTTCTTGAA+TGG 0.411936 1_3:+8883010 None:intergenic
GGAACGGAATAATGCACTTA+TGG 0.418242 1_3:-8882919 Msa0102160:CDS
CGAGAAAATGCTTTGGCCAA+TGG 0.428561 1_3:-8882864 Msa0102160:CDS
GTATCGATTTGTACCATGTT+TGG 0.434696 1_3:+8882161 None:intergenic
TTCCCAACCTCTGAAGAAAT+GGG 0.437141 1_3:+8882690 None:intergenic
GTTGATTCGGATTCAGTCGC+TGG 0.437150 1_3:-8883569 Msa0102160:five_prime_UTR
AGGTTTCCAATCAGCCTTTG+AGG 0.441798 1_3:+8882473 None:intergenic
TTGATCATGTATTGCCCATC+CGG 0.447387 1_3:+8882105 None:intergenic
TATACATCACCTTCCGCAAC+AGG 0.447735 1_3:-8882790 Msa0102160:CDS
AATCACCCATATTAGGCAAA+TGG 0.448313 1_3:+8882763 None:intergenic
TTGTACCATGTTTGGCCCAA+TGG 0.454297 1_3:+8882169 None:intergenic
ATAATTAGGATCCAAGGAAA+TGG 0.454358 1_3:-8881994 Msa0102160:three_prime_UTR
TAGCAGCCTCAAAGGCTGAT+TGG 0.454649 1_3:-8882479 Msa0102160:CDS
AATATGGGTGATTCTTCTTA+CGG 0.455899 1_3:-8882753 Msa0102160:CDS
AGATCAATATAAATCTGCTC+AGG 0.457509 1_3:-8883239 Msa0102160:CDS
TTCTGAGACAGTGCTACAAA+TGG 0.466001 1_3:-8882377 Msa0102160:CDS
GTACAAGAGCATGCTGGTGC+TGG 0.473862 1_3:+8882405 None:intergenic
GCACCAGCATGCTCTTGTAC+TGG 0.474798 1_3:-8882402 Msa0102160:CDS
CGGTTGACCTTAAAGACCAT+TGG 0.478594 1_3:-8882185 Msa0102160:CDS
TCTGAGACAGTGCTACAAAT+GGG 0.478630 1_3:-8882376 Msa0102160:CDS
GTAAACCATTTGCCTAATAT+GGG 0.479892 1_3:-8882768 Msa0102160:CDS
CAGTGCTACAAATGGGGTAA+TGG 0.484703 1_3:-8882369 Msa0102160:CDS
TCAGTTTCGAGAAAATGCTT+TGG 0.485734 1_3:-8882871 Msa0102160:CDS
AGCCATTCAAGAAAGAAATC+TGG 0.489436 1_3:-8883012 Msa0102160:CDS
CCCCGTGAGATTTGACATCC+TGG 0.492305 1_3:+8882822 None:intergenic
TGATTGGAAACCTCAGGACT+TGG 0.494236 1_3:-8882463 Msa0102160:CDS
GGCAAATGGTTTACCTGTTG+CGG 0.495581 1_3:+8882777 None:intergenic
TTCCAGCTGTGCCCAACAAA+AGG 0.495805 1_3:-8882299 Msa0102160:CDS
TTGCAGGTCAAAATGAATAC+CGG 0.497288 1_3:+8881952 None:intergenic
GAAGCAAATTATGTCAATCA+TGG 0.497300 1_3:-8883048 Msa0102160:CDS
CTTAGCCGGCTTGCAGCTGA+AGG 0.500276 1_3:-8882225 Msa0102160:CDS
CGTCCATCAGAATTAACAAA+TGG 0.501557 1_3:+8883287 None:intergenic
TTGAATCATCGTATGCAACT+TGG 0.503216 1_3:+8882433 None:intergenic
GACATAATTTGCTTCATGGA+AGG 0.504898 1_3:+8883056 None:intergenic
ATATAAATCTGCTCAGGGAC+AGG 0.509112 1_3:-8883233 Msa0102160:intron
CTTTCAATAAACTACTTAGC+CGG 0.514461 1_3:-8882239 Msa0102160:CDS
TTTGGCCGTTTGGCACGCCT+AGG 0.515774 1_3:+8882663 None:intergenic
TTGTTGGGCACAGCTGGAAG+TGG 0.517836 1_3:+8882303 None:intergenic
GGTATGTCATCATGCCCTCC+AGG 0.518820 1_3:-8882840 Msa0102160:CDS
TTGATTGACCGAGTTGGATG+CGG 0.521545 1_3:-8883348 Msa0102160:five_prime_UTR
GGTTGACCTTAAAGACCATT+GGG 0.521688 1_3:-8882184 Msa0102160:CDS
GGATGGGCAATACATGATCA+AGG 0.528445 1_3:-8882103 Msa0102160:three_prime_UTR
GGCTTGATTAATACATCTTG+TGG 0.531451 1_3:-8881895 Msa0102160:three_prime_UTR
CAAGCAGTTAGCAGCCTCAA+AGG 0.533010 1_3:-8882487 Msa0102160:CDS
AAAGGCTGATTGGAAACCTC+AGG 0.538629 1_3:-8882469 Msa0102160:CDS
AACCACCTAGGCGTGCCAAA+CGG 0.540428 1_3:-8882668 Msa0102160:CDS
TGATTGACCGAGTTGGATGC+GGG 0.542081 1_3:-8883347 Msa0102160:five_prime_UTR
CAATTGCAGTATCTCGCTGC+AGG 0.542654 1_3:+8882943 None:intergenic
ACAAATGGGGTAATGGAGGT+TGG 0.545815 1_3:-8882362 Msa0102160:CDS
ATCTAGAAAAGTCAAGAAAG+AGG 0.546447 1_3:-8882601 Msa0102160:CDS
GGCCGTTTGGCACGCCTAGG+TGG 0.547084 1_3:+8882666 None:intergenic
ATTCCTATAATTAGGATCCA+AGG 0.551896 1_3:-8882000 Msa0102160:three_prime_UTR
TCCAGGATGTCAAATCTCAC+GGG 0.552910 1_3:-8882823 Msa0102160:CDS
CAAGATTCAACTAAAGTACA+AGG 0.554025 1_3:+8881847 None:intergenic
ACATGATCAAGGGTCAGCTA+TGG 0.554160 1_3:-8882092 Msa0102160:three_prime_UTR
CTCCAGGATGTCAAATCTCA+CGG 0.563255 1_3:-8882824 Msa0102160:CDS
TTGATTCGGATTCAGTCGCT+GGG 0.566827 1_3:-8883568 Msa0102160:five_prime_UTR
GCATGATGACATACCACCAT+TGG 0.567052 1_3:+8882848 None:intergenic
TATTGCTTGATTGACCGAGT+TGG 0.567676 1_3:-8883354 Msa0102160:five_prime_UTR
TCCTAATCCCGCATCCAACT+CGG 0.576248 1_3:+8883340 None:intergenic
TTGATTAATACATCTTGTGG+TGG 0.577501 1_3:-8881892 Msa0102160:three_prime_UTR
TTTGCTTCATGGAAGGATGC+TGG 0.583298 1_3:+8883063 None:intergenic
TGGTTCAATGCCAAGTCCTG+AGG 0.592965 1_3:+8882453 None:intergenic
CAATGTAAAACGTATGTCAC+CGG 0.595449 1_3:-8881971 Msa0102160:three_prime_UTR
AGAGCATGCTGGTGCTGGCA+TGG 0.596415 1_3:+8882410 None:intergenic
CCCAACCTCTGAAGAAATGG+GGG 0.597363 1_3:+8882692 None:intergenic
GCTTGAATGGAAGAGTAGTG+AGG 0.599481 1_3:-8882538 Msa0102160:CDS
AAAAGGCATGCTCGAGTAGG+TGG 0.599819 1_3:-8882282 Msa0102160:CDS
TAGGCGTGCCAAACGGCCAA+AGG 0.600212 1_3:-8882661 Msa0102160:CDS
CATGCCCTTCAGCTGCAAGC+CGG 0.601437 1_3:+8882220 None:intergenic
AAAGACCATTGGGCCAAACA+TGG 0.604624 1_3:-8882174 Msa0102160:CDS
AAGTGGATATACCGATAAAG+TGG 0.604637 1_3:+8882320 None:intergenic
TCTAGAAAAGTCAAGAAAGA+GGG 0.605111 1_3:-8882600 Msa0102160:CDS
AATGGTTTACCTGTTGCGGA+AGG 0.606017 1_3:+8882781 None:intergenic
GAGATACTGCAATTGCGGAA+CGG 0.606489 1_3:-8882935 Msa0102160:CDS
TGCTACAAATGGGGTAATGG+AGG 0.606890 1_3:-8882366 Msa0102160:CDS
TCCCAACCTCTGAAGAAATG+GGG 0.610433 1_3:+8882691 None:intergenic
GATCAATATAAATCTGCTCA+GGG 0.611740 1_3:-8883238 Msa0102160:CDS
GCAGCGAGATACTGCAATTG+CGG 0.613903 1_3:-8882940 Msa0102160:CDS
GAAAATGCTTTGGCCAATGG+TGG 0.615877 1_3:-8882861 Msa0102160:CDS
CAGAGGTTGGGAAACCACCT+AGG 0.616912 1_3:-8882680 Msa0102160:CDS
AGCACTTGCAGAACGTGACA+TGG 0.620297 1_3:-8882970 Msa0102160:CDS
TGGACGATCGTGAAAATGGA+AGG 0.626784 1_3:-8883270 Msa0102160:CDS
TTAGCCGGCTTGCAGCTGAA+GGG 0.630246 1_3:-8882224 Msa0102160:CDS
GTTGGATGCGGGATTAGGAG+AGG 0.635531 1_3:-8883336 Msa0102160:five_prime_UTR
CTGAGACAGTGCTACAAATG+GGG 0.642920 1_3:-8882375 Msa0102160:CDS
AGGGCATGATCTCTCACACC+CGG 0.643910 1_3:-8882205 Msa0102160:CDS
ACACCAGTACAAGAGCATGC+TGG 0.644328 1_3:+8882399 None:intergenic
ATAGCTTCTATGTACAAGCA+TGG 0.646465 1_3:-8883542 Msa0102160:intron
GTGAGATTTGACATCCTGGA+GGG 0.655568 1_3:+8882826 None:intergenic
GATGGGCAATACATGATCAA+GGG 0.656643 1_3:-8882102 Msa0102160:three_prime_UTR
AACAAAAGGCATGCTCGAGT+AGG 0.658830 1_3:-8882285 Msa0102160:CDS
CCAGGATGTCAAATCTCACG+GGG 0.670697 1_3:-8882822 Msa0102160:CDS
AATGGTCTTTAAGGTCAACC+GGG 0.672212 1_3:+8882187 None:intergenic
ATGGATTCTAAAGTTGAACA+AGG 0.676747 1_3:-8882073 Msa0102160:three_prime_UTR
AAATGCGATTGCCACCACCA+CGG 0.689345 1_3:+8881922 None:intergenic
CGTGAGATTTGACATCCTGG+AGG 0.739694 1_3:+8882825 None:intergenic
GATTCTAAAGTTGAACAAGG+AGG 0.751080 1_3:-8882070 Msa0102160:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCTTAAATGAAAGAAAAAA+AGG + chr1_3:8881781-8881800 None:intergenic 15.0%
!!! AATTTTTTGAAGAGTTGATT+CGG - chr1_3:8881898-8881917 Msa0102160:three_prime_UTR 20.0%
!!! TTAAATTTATTTCTGAGCAG+AGG + chr1_3:8882286-8882305 None:intergenic 25.0%
!! TTCTTGACTTTTCTAGATTT+TGG + chr1_3:8882877-8882896 None:intergenic 25.0%
!! AATGTGTCATTCCTATAATT+AGG - chr1_3:8883472-8883491 Msa0102160:intron 25.0%
!! ATTCGAGTTTTTATCATAGC+TGG - chr1_3:8881818-8881837 Msa0102160:three_prime_UTR 30.0%
! CAGAAAAATTTGATTCGAGT+TGG + chr1_3:8881871-8881890 None:intergenic 30.0%
! TCGAATCAAATTTTTCTGCA+GGG - chr1_3:8881873-8881892 Msa0102160:three_prime_UTR 30.0%
ACAACACTAACAAAGAATAG+AGG + chr1_3:8881985-8882004 None:intergenic 30.0%
CTATTCTTTGTTAGTGTTGT+TGG - chr1_3:8881985-8882004 Msa0102160:three_prime_UTR 30.0%
TAGTTCCTTAATTGACATTC+AGG - chr1_3:8882090-8882109 Msa0102160:three_prime_UTR 30.0%
! CTACCATTTGTTAATTCTGA+TGG - chr1_3:8882190-8882209 Msa0102160:CDS 30.0%
AGATCAATATAAATCTGCTC+AGG - chr1_3:8882241-8882260 Msa0102160:CDS 30.0%
GATCAATATAAATCTGCTCA+GGG - chr1_3:8882242-8882261 Msa0102160:CDS 30.0%
!! TCTAAAAATTGCTTTTGCAG+TGG - chr1_3:8882377-8882396 Msa0102160:CDS 30.0%
!! GATTGACATAATTTGCTTCA+TGG + chr1_3:8882431-8882450 None:intergenic 30.0%
GAAGCAAATTATGTCAATCA+TGG - chr1_3:8882432-8882451 Msa0102160:CDS 30.0%
GTAAACCATTTGCCTAATAT+GGG - chr1_3:8882712-8882731 Msa0102160:CDS 30.0%
TAAGAAGAATCACCCATATT+AGG + chr1_3:8882727-8882746 None:intergenic 30.0%
AATATGGGTGATTCTTCTTA+CGG - chr1_3:8882727-8882746 Msa0102160:CDS 30.0%
ATCTAGAAAAGTCAAGAAAG+AGG - chr1_3:8882879-8882898 Msa0102160:CDS 30.0%
TCTAGAAAAGTCAAGAAAGA+GGG - chr1_3:8882880-8882899 Msa0102160:CDS 30.0%
AGTAGTGAGGAAATGATAAA+TGG - chr1_3:8882955-8882974 Msa0102160:CDS 30.0%
CTTTCAATAAACTACTTAGC+CGG - chr1_3:8883241-8883260 Msa0102160:CDS 30.0%
!! ATGGATTCTAAAGTTGAACA+AGG - chr1_3:8883407-8883426 Msa0102160:intron 30.0%
ATTCCTATAATTAGGATCCA+AGG - chr1_3:8883480-8883499 Msa0102160:intron 30.0%
TTTCCTTGGATCCTAATTAT+AGG + chr1_3:8883486-8883505 None:intergenic 30.0%
ATAATTAGGATCCAAGGAAA+TGG - chr1_3:8883486-8883505 Msa0102160:intron 30.0%
!! TTCATTTTGACCTGCAAAAA+TGG - chr1_3:8883534-8883553 Msa0102160:intron 30.0%
TTGATTAATACATCTTGTGG+TGG - chr1_3:8883588-8883607 Msa0102160:five_prime_UTR 30.0%
CAAGATTCAACTAAAGTACA+AGG + chr1_3:8883636-8883655 None:intergenic 30.0%
! CTCGAATCAAATTTTTCTGC+AGG - chr1_3:8881872-8881891 Msa0102160:three_prime_UTR 35.0%
ATAGCTTCTATGTACAAGCA+TGG - chr1_3:8881938-8881957 Msa0102160:three_prime_UTR 35.0%
AATAGAGGTGGAAAAGGTTA+TGG + chr1_3:8881970-8881989 None:intergenic 35.0%
ACAAAGAATAGAGGTGGAAA+AGG + chr1_3:8881976-8881995 None:intergenic 35.0%
ACACTAACAAAGAATAGAGG+TGG + chr1_3:8881982-8882001 None:intergenic 35.0%
CAAGTCCTGAATGTCAATTA+AGG + chr1_3:8882098-8882117 None:intergenic 35.0%
CGTCCATCAGAATTAACAAA+TGG + chr1_3:8882196-8882215 None:intergenic 35.0%
! AAGTGCTGCATATACCTAAA+AGG - chr1_3:8882332-8882351 Msa0102160:CDS 35.0%
AGTGCTGCATATACCTAAAA+GGG - chr1_3:8882333-8882352 Msa0102160:CDS 35.0%
!! GACATAATTTGCTTCATGGA+AGG + chr1_3:8882427-8882446 None:intergenic 35.0%
AGCCATTCAAGAAAGAAATC+TGG - chr1_3:8882468-8882487 Msa0102160:CDS 35.0%
! AGCATTTTCTCGAAACTGAA+GGG + chr1_3:8882608-8882627 None:intergenic 35.0%
! AAGCATTTTCTCGAAACTGA+AGG + chr1_3:8882609-8882628 None:intergenic 35.0%
TCAGTTTCGAGAAAATGCTT+TGG - chr1_3:8882609-8882628 Msa0102160:CDS 35.0%
GGTAAACCATTTGCCTAATA+TGG - chr1_3:8882711-8882730 Msa0102160:CDS 35.0%
AATCACCCATATTAGGCAAA+TGG + chr1_3:8882720-8882739 None:intergenic 35.0%
GAATCAGACTTACTTTCCTT+TGG + chr1_3:8882838-8882857 None:intergenic 35.0%
!!! GATTTTGGAGTCTTCTTGTT+AGG + chr1_3:8882862-8882881 None:intergenic 35.0%
TTGAATCATCGTATGCAACT+TGG + chr1_3:8883050-8883069 None:intergenic 35.0%
TTGCTGTACAACCACTTTAT+CGG - chr1_3:8883149-8883168 Msa0102160:intron 35.0%
AAGTGGATATACCGATAAAG+TGG + chr1_3:8883163-8883182 None:intergenic 35.0%
!! GTATCGATTTGTACCATGTT+TGG + chr1_3:8883322-8883341 None:intergenic 35.0%
! GACAACAATGCATTTTTCTC+CGG - chr1_3:8883356-8883375 Msa0102160:five_prime_UTR 35.0%
!! GATTCTAAAGTTGAACAAGG+AGG - chr1_3:8883410-8883429 Msa0102160:intron 35.0%
GTTGAACAAGGAGGAAAATA+AGG - chr1_3:8883419-8883438 Msa0102160:intron 35.0%
!! GTTTTACATTGCCATTTCCT+TGG + chr1_3:8883500-8883519 None:intergenic 35.0%
CAATGTAAAACGTATGTCAC+CGG - chr1_3:8883509-8883528 Msa0102160:intron 35.0%
TTGCAGGTCAAAATGAATAC+CGG + chr1_3:8883531-8883550 None:intergenic 35.0%
GGCTTGATTAATACATCTTG+TGG - chr1_3:8883585-8883604 Msa0102160:five_prime_UTR 35.0%
TATTGCTTGATTGACCGAGT+TGG - chr1_3:8882126-8882145 Msa0102160:three_prime_UTR 40.0%
ATATAAATCTGCTCAGGGAC+AGG - chr1_3:8882247-8882266 Msa0102160:CDS 40.0%
!!! AGTAGAGAAAGCACCCTTTT+AGG + chr1_3:8882349-8882368 None:intergenic 40.0%
GGCCAGATTTCTTTCTTGAA+TGG + chr1_3:8882473-8882492 None:intergenic 40.0%
GGAACGGAATAATGCACTTA+TGG - chr1_3:8882561-8882580 Msa0102160:CDS 40.0%
! TTCCCAACCTCTGAAGAAAT+GGG + chr1_3:8882793-8882812 None:intergenic 40.0%
! TTTCCCAACCTCTGAAGAAA+TGG + chr1_3:8882794-8882813 None:intergenic 40.0%
! CCATTCAAGCTCTTTGTTGT+TGG + chr1_3:8882932-8882951 None:intergenic 40.0%
CCAACAACAAAGAGCTTGAA+TGG - chr1_3:8882929-8882948 Msa0102160:CDS 40.0%
!! TTCTGAGACAGTGCTACAAA+TGG - chr1_3:8883103-8883122 Msa0102160:intron 40.0%
! TCTGAGACAGTGCTACAAAT+GGG - chr1_3:8883104-8883123 Msa0102160:intron 40.0%
GTAGGTGGTAGAAAAATGAG+TGG - chr1_3:8883213-8883232 Msa0102160:intron 40.0%
!! AATGGTCTTTAAGGTCAACC+GGG + chr1_3:8883296-8883315 None:intergenic 40.0%
!! CAATGGTCTTTAAGGTCAAC+CGG + chr1_3:8883297-8883316 None:intergenic 40.0%
GGTTGACCTTAAAGACCATT+GGG - chr1_3:8883296-8883315 Msa0102160:five_prime_UTR 40.0%
! ACAATGCATTTTTCTCCGGA+TGG - chr1_3:8883360-8883379 Msa0102160:five_prime_UTR 40.0%
! CAATGCATTTTTCTCCGGAT+GGG - chr1_3:8883361-8883380 Msa0102160:five_prime_UTR 40.0%
TTGATCATGTATTGCCCATC+CGG + chr1_3:8883378-8883397 None:intergenic 40.0%
GATGGGCAATACATGATCAA+GGG - chr1_3:8883378-8883397 Msa0102160:intron 40.0%
TGGTGGCAATCGCATTTAAT+AGG - chr1_3:8883564-8883583 Msa0102160:five_prime_UTR 40.0%
!! TTGATTCGGATTCAGTCGCT+GGG - chr1_3:8881912-8881931 Msa0102160:three_prime_UTR 45.0%
! TTGATTGACCGAGTTGGATG+CGG - chr1_3:8882132-8882151 Msa0102160:exon 45.0%
! CTGATGGACGATCGTGAAAA+TGG - chr1_3:8882206-8882225 Msa0102160:CDS 45.0%
TGGACGATCGTGAAAATGGA+AGG - chr1_3:8882210-8882229 Msa0102160:CDS 45.0%
TTTGCTTCATGGAAGGATGC+TGG + chr1_3:8882420-8882439 None:intergenic 45.0%
! TGCTGCCTTTTTCTCAGAGA+GGG + chr1_3:8882494-8882513 None:intergenic 45.0%
GAGATACTGCAATTGCGGAA+CGG - chr1_3:8882545-8882564 Msa0102160:CDS 45.0%
CGAGAAAATGCTTTGGCCAA+TGG - chr1_3:8882616-8882635 Msa0102160:CDS 45.0%
GAAAATGCTTTGGCCAATGG+TGG - chr1_3:8882619-8882638 Msa0102160:CDS 45.0%
GCATGATGACATACCACCAT+TGG + chr1_3:8882635-8882654 None:intergenic 45.0%
GTGAGATTTGACATCCTGGA+GGG + chr1_3:8882657-8882676 None:intergenic 45.0%
CTCCAGGATGTCAAATCTCA+CGG - chr1_3:8882656-8882675 Msa0102160:CDS 45.0%
TCCAGGATGTCAAATCTCAC+GGG - chr1_3:8882657-8882676 Msa0102160:CDS 45.0%
TATACATCACCTTCCGCAAC+AGG - chr1_3:8882690-8882709 Msa0102160:CDS 45.0%
AATGGTTTACCTGTTGCGGA+AGG + chr1_3:8882702-8882721 None:intergenic 45.0%
GGCAAATGGTTTACCTGTTG+CGG + chr1_3:8882706-8882725 None:intergenic 45.0%
! TCCCAACCTCTGAAGAAATG+GGG + chr1_3:8882792-8882811 None:intergenic 45.0%
CTTACTTTCCTTTGGCCGTT+TGG + chr1_3:8882830-8882849 None:intergenic 45.0%
GCTTGAATGGAAGAGTAGTG+AGG - chr1_3:8882942-8882961 Msa0102160:CDS 45.0%
AGGTTTCCAATCAGCCTTTG+AGG + chr1_3:8883010-8883029 None:intergenic 45.0%
AAAGGCTGATTGGAAACCTC+AGG - chr1_3:8883011-8883030 Msa0102160:CDS 45.0%
TGATTGGAAACCTCAGGACT+TGG - chr1_3:8883017-8883036 Msa0102160:CDS 45.0%
CTGAGACAGTGCTACAAATG+GGG - chr1_3:8883105-8883124 Msa0102160:intron 45.0%
CAGTGCTACAAATGGGGTAA+TGG - chr1_3:8883111-8883130 Msa0102160:intron 45.0%
! TGCTACAAATGGGGTAATGG+AGG - chr1_3:8883114-8883133 Msa0102160:intron 45.0%
!! ACAAATGGGGTAATGGAGGT+TGG - chr1_3:8883118-8883137 Msa0102160:intron 45.0%
! ACTCGAGCATGCCTTTTGTT+GGG + chr1_3:8883195-8883214 None:intergenic 45.0%
! TACTCGAGCATGCCTTTTGT+TGG + chr1_3:8883196-8883215 None:intergenic 45.0%
! AACAAAAGGCATGCTCGAGT+AGG - chr1_3:8883195-8883214 Msa0102160:intron 45.0%
CGGTTGACCTTAAAGACCAT+TGG - chr1_3:8883295-8883314 Msa0102160:five_prime_UTR 45.0%
GTTTGGCCCAATGGTCTTTA+AGG + chr1_3:8883305-8883324 None:intergenic 45.0%
AAAGACCATTGGGCCAAACA+TGG - chr1_3:8883306-8883325 Msa0102160:five_prime_UTR 45.0%
TTGTACCATGTTTGGCCCAA+TGG + chr1_3:8883314-8883333 None:intergenic 45.0%
GGATGGGCAATACATGATCA+AGG - chr1_3:8883377-8883396 Msa0102160:intron 45.0%
ACATGATCAAGGGTCAGCTA+TGG - chr1_3:8883388-8883407 Msa0102160:intron 45.0%
!! GTTGATTCGGATTCAGTCGC+TGG - chr1_3:8881911-8881930 Msa0102160:three_prime_UTR 50.0%
ACAAATTTCCTCCACCCTCC+AGG - chr1_3:8882011-8882030 Msa0102160:three_prime_UTR 50.0%
CTTTCCTCCTGTGTTCCTTG+AGG + chr1_3:8882037-8882056 None:intergenic 50.0%
! TGATTGACCGAGTTGGATGC+GGG - chr1_3:8882133-8882152 Msa0102160:exon 50.0%
TCCTAATCCCGCATCCAACT+CGG + chr1_3:8882143-8882162 None:intergenic 50.0%
! TCTGGCCCTCTCTGAGAAAA+AGG - chr1_3:8882486-8882505 Msa0102160:CDS 50.0%
! GTGCTGCCTTTTTCTCAGAG+AGG + chr1_3:8882495-8882514 None:intergenic 50.0%
AGCACTTGCAGAACGTGACA+TGG - chr1_3:8882510-8882529 Msa0102160:CDS 50.0%
CAATTGCAGTATCTCGCTGC+AGG + chr1_3:8882540-8882559 None:intergenic 50.0%
GCAGCGAGATACTGCAATTG+CGG - chr1_3:8882540-8882559 Msa0102160:CDS 50.0%
CGTGAGATTTGACATCCTGG+AGG + chr1_3:8882658-8882677 None:intergenic 50.0%
CCAGGATGTCAAATCTCACG+GGG - chr1_3:8882658-8882677 Msa0102160:CDS 50.0%
! CCCAACCTCTGAAGAAATGG+GGG + chr1_3:8882791-8882810 None:intergenic 50.0%
CCCCCATTTCTTCAGAGGTT+GGG - chr1_3:8882788-8882807 Msa0102160:CDS 50.0%
CAAGCAGTTAGCAGCCTCAA+AGG - chr1_3:8882993-8883012 Msa0102160:CDS 50.0%
TAGCAGCCTCAAAGGCTGAT+TGG - chr1_3:8883001-8883020 Msa0102160:CDS 50.0%
TGGTTCAATGCCAAGTCCTG+AGG + chr1_3:8883030-8883049 None:intergenic 50.0%
! ACACCAGTACAAGAGCATGC+TGG + chr1_3:8883084-8883103 None:intergenic 50.0%
TTCCAGCTGTGCCCAACAAA+AGG - chr1_3:8883181-8883200 Msa0102160:intron 50.0%
! AAAAGGCATGCTCGAGTAGG+TGG - chr1_3:8883198-8883217 Msa0102160:intron 50.0%
TGACCTGCAAAAATGGTCCG+TGG - chr1_3:8883541-8883560 Msa0102160:intron 50.0%
AAATGCGATTGCCACCACCA+CGG + chr1_3:8883561-8883580 None:intergenic 50.0%
TGTGTTCCTTGAGGCCTGGA+GGG + chr1_3:8882028-8882047 None:intergenic 55.0%
! ACCGAGTTGGATGCGGGATT+AGG - chr1_3:8882139-8882158 Msa0102160:exon 55.0%
GTTGGATGCGGGATTAGGAG+AGG - chr1_3:8882144-8882163 Msa0102160:exon 55.0%
GGTATGTCATCATGCCCTCC+AGG - chr1_3:8882640-8882659 Msa0102160:CDS 55.0%
CCCCGTGAGATTTGACATCC+TGG + chr1_3:8882661-8882680 None:intergenic 55.0%
CTGAAGAAATGGGGGCTGCT+GGG + chr1_3:8882783-8882802 None:intergenic 55.0%
TCTGAAGAAATGGGGGCTGC+TGG + chr1_3:8882784-8882803 None:intergenic 55.0%
AGCAGCCCCCATTTCTTCAG+AGG - chr1_3:8882783-8882802 Msa0102160:CDS 55.0%
GCCCCCATTTCTTCAGAGGT+TGG - chr1_3:8882787-8882806 Msa0102160:CDS 55.0%
CAGAGGTTGGGAAACCACCT+AGG - chr1_3:8882800-8882819 Msa0102160:CDS 55.0%
AACCACCTAGGCGTGCCAAA+CGG - chr1_3:8882812-8882831 Msa0102160:CDS 55.0%
! GTACAAGAGCATGCTGGTGC+TGG + chr1_3:8883078-8883097 None:intergenic 55.0%
GCACCAGCATGCTCTTGTAC+TGG - chr1_3:8883078-8883097 Msa0102160:CDS 55.0%
TTGTTGGGCACAGCTGGAAG+TGG + chr1_3:8883180-8883199 None:intergenic 55.0%
! TGCCTTTTGTTGGGCACAGC+TGG + chr1_3:8883186-8883205 None:intergenic 55.0%
!! TTAGCCGGCTTGCAGCTGAA+GGG - chr1_3:8883256-8883275 Msa0102160:CDS 55.0%
AGGGCATGATCTCTCACACC+CGG - chr1_3:8883275-8883294 Msa0102160:exon 55.0%
!! CCACCACGGACCATTTTTGC+AGG + chr1_3:8883547-8883566 None:intergenic 55.0%
CCTGCAAAAATGGTCCGTGG+TGG - chr1_3:8883544-8883563 Msa0102160:five_prime_UTR 55.0%
GCAAAAATGGTCCGTGGTGG+TGG - chr1_3:8883547-8883566 Msa0102160:five_prime_UTR 55.0%
CTGTGTTCCTTGAGGCCTGG+AGG + chr1_3:8882029-8882048 None:intergenic 60.0%
CTCCAGGCCTCAAGGAACAC+AGG - chr1_3:8882027-8882046 Msa0102160:three_prime_UTR 60.0%
CTCCTGTGTTCCTTGAGGCC+TGG + chr1_3:8882032-8882051 None:intergenic 60.0%
CAGGCCTCAAGGAACACAGG+AGG - chr1_3:8882030-8882049 Msa0102160:three_prime_UTR 60.0%
AGAAATGGGGGCTGCTGGGA+GGG + chr1_3:8882779-8882798 None:intergenic 60.0%
AAGAAATGGGGGCTGCTGGG+AGG + chr1_3:8882780-8882799 None:intergenic 60.0%
TTTGGCCGTTTGGCACGCCT+AGG + chr1_3:8882820-8882839 None:intergenic 60.0%
TAGGCGTGCCAAACGGCCAA+AGG - chr1_3:8882819-8882838 Msa0102160:CDS 60.0%
!! AGAGCATGCTGGTGCTGGCA+TGG + chr1_3:8883073-8883092 None:intergenic 60.0%
!! CTTAGCCGGCTTGCAGCTGA+AGG - chr1_3:8883255-8883274 Msa0102160:CDS 60.0%
CATGCCCTTCAGCTGCAAGC+CGG + chr1_3:8883263-8883282 None:intergenic 60.0%
GTTCCTTGAGGCCTGGAGGG+TGG + chr1_3:8882025-8882044 None:intergenic 65.0%
CCTTGAGGCCTGGAGGGTGG+AGG + chr1_3:8882022-8882041 None:intergenic 70.0%
CCTCCACCCTCCAGGCCTCA+AGG - chr1_3:8882019-8882038 Msa0102160:three_prime_UTR 70.0%
GGCCGTTTGGCACGCCTAGG+TGG + chr1_3:8882817-8882836 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 8881770 8883732 8881770 ID=Msa0102160;Name=Msa0102160
chr1_3 mRNA 8881770 8883732 8881770 ID=Msa0102160-mRNA-1;Parent=Msa0102160;Name=Msa0102160-mRNA-1;_AED=0.01;_eAED=0.01;_QI=287|1|1|1|0.5|0.33|3|376|339
chr1_3 exon 8881770 8883105 8881770 ID=Msa0102160-mRNA-1:exon:3527;Parent=Msa0102160-mRNA-1
chr1_3 exon 8883234 8883390 8883234 ID=Msa0102160-mRNA-1:exon:3526;Parent=Msa0102160-mRNA-1
chr1_3 exon 8883543 8883732 8883543 ID=Msa0102160-mRNA-1:exon:3525;Parent=Msa0102160-mRNA-1
chr1_3 five_prime_UTR 8883543 8883732 8883543 ID=Msa0102160-mRNA-1:five_prime_utr;Parent=Msa0102160-mRNA-1
chr1_3 five_prime_UTR 8883294 8883390 8883294 ID=Msa0102160-mRNA-1:five_prime_utr;Parent=Msa0102160-mRNA-1
chr1_3 CDS 8883234 8883293 8883234 ID=Msa0102160-mRNA-1:cds;Parent=Msa0102160-mRNA-1
chr1_3 CDS 8882146 8883105 8882146 ID=Msa0102160-mRNA-1:cds;Parent=Msa0102160-mRNA-1
chr1_3 three_prime_UTR 8881770 8882145 8881770 ID=Msa0102160-mRNA-1:three_prime_utr;Parent=Msa0102160-mRNA-1
Gene Sequence

>Msa0102160

ATGGACGATCGTGAAAATGGAAGGCACAAAGCAGATCAATATAAATCTGCTCAGGGACAGTGGTTAATGCAACAGCATCACCAGCATCCTTCCATGAAGCAAATTATGTCAATCATGGCTGAACGAGATGCAGCCATTCAAGAAAGAAATCTGGCCCTCTCTGAGAAAAAGGCAGCACTTGCAGAACGTGACATGGCATTCCTGCAGCGAGATACTGCAATTGCGGAACGGAATAATGCACTTATGGAAAGAGATAATGCGATTGCAACCCTTCAGTTTCGAGAAAATGCTTTGGCCAATGGTGGTATGTCATCATGCCCTCCAGGATGTCAAATCTCACGGGGAGTTAAGCATATACATCACCTTCCGCAACAGGTAAACCATTTGCCTAATATGGGTGATTCTTCTTACGGCACACGTGAATTGCACACAACTGATGCCCTCCCAGCAGCCCCCATTTCTTCAGAGGTTGGGAAACCACCTAGGCGTGCCAAACGGCCAAAGGAAAGTAAGTCTGATTCACCTAACAAGAAGACTCCAAAATCTAGAAAAGTCAAGAAAGAGGGTGATGATTTAAACAAGACAATGTTTGCCAACAACAAAGAGCTTGAATGGAAGAGTAGTGAGGAAATGATAAATGGAGATGATGATCTTAACAAGCAGTTAGCAGCCTCAAAGGCTGATTGGAAACCTCAGGACTTGGCATTGAACCAAGTTGCATACGATGATTCAACCATGCCAGCACCAGCATGCTCTTGTACTGGTGTTCTGAGACAGTGCTACAAATGGGGTAATGGAGGTTGGCAATCTGCTTGCTGTACAACCACTTTATCGGTATATCCACTTCCAGCTGTGCCCAACAAAAGGCATGCTCGAGTAGGTGGTAGAAAAATGAGTGGAAGTGCTTTCAATAAACTACTTAGCCGGCTTGCAGCTGAAGGGCATGATCTCTCACACCCGGTTGACCTTAAAGACCATTGGGCCAAACATGGTACAAATCGATACATCACAATAAAGTAG
Protein sequence

>Msa0102160

MDDRENGRHKADQYKSAQGQWLMQQHHQHPSMKQIMSIMAERDAAIQERNLALSEKKAALAERDMAFLQRDTAIAERNNALMERDNAIATLQFRENALANGGMSSCPPGCQISRGVKHIHHLPQQVNHLPNMGDSSYGTRELHTTDALPAAPISSEVGKPPRRAKRPKESKSDSPNKKTPKSRKVKKEGDDLNKTMFANNKELEWKSSEEMINGDDDLNKQLAASKADWKPQDLALNQVAYDDSTMPAPACSCTGVLRQCYKWGNGGWQSACCTTTLSVYPLPAVPNKRHARVGGRKMSGSAFNKLLSRLAAEGHDLSHPVDLKDHWAKHGTNRYITIK*