Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0103260 | Msa0347230 | 0.881320 | 2.647586e-70 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATATAGATGTTGATTGAAAT+TGG | 0.232139 | 1_3:-10403320 | None:intergenic |
| TGTGAGTTGTTACGTGAAAT+AGG | 0.281469 | 1_3:-10403234 | None:intergenic |
| AAGGAGAGGAGCACGGAAAC+TGG | 0.341508 | 1_3:+10403447 | Msa0103260:five_prime_UTR |
| GTTTCCGTGCTCCTCTCCTT+TGG | 0.345017 | 1_3:-10403444 | None:intergenic |
| GTGTGTTGTTATGCTGGATT+CGG | 0.357976 | 1_3:-10403282 | None:intergenic |
| AGCTGAACCGGTTCAGGAAC+CGG | 0.361281 | 1_3:-10403756 | None:intergenic |
| AAGAAAAGCTGAACCGGTTC+AGG | 0.362146 | 1_3:-10403762 | None:intergenic |
| TACCTCTTCCTTCTCAGCTT+CGG | 0.371599 | 1_3:-10403529 | None:intergenic |
| TTGTATTTGCTGAACCGGTT+CGG | 0.381246 | 1_3:+10403723 | Msa0103260:CDS |
| ATAGAGAGAGAGAAGAGAAT+TGG | 0.384503 | 1_3:+10403628 | Msa0103260:intron |
| AACCGACCCGGTTCCTGAAC+CGG | 0.403375 | 1_3:+10403749 | Msa0103260:CDS |
| TGTGTTGTTATGCTGGATTC+GGG | 0.411143 | 1_3:-10403281 | None:intergenic |
| GCTTATGTGTGTTGTTATGC+TGG | 0.414463 | 1_3:-10403288 | None:intergenic |
| AAGGAGTAAAACTGTGGGCT+TGG | 0.438906 | 1_3:-10403789 | None:intergenic |
| TTCGTTCCATCAGTTCGAAA+TGG | 0.443647 | 1_3:-10403380 | None:intergenic |
| CGCAAGCCATTTCGAACTGA+TGG | 0.458753 | 1_3:+10403374 | Msa0103260:five_prime_UTR |
| TCTCCTTTGGATCGAACAAA+AGG | 0.474957 | 1_3:-10403431 | None:intergenic |
| AGAGAGAAGAGAATTGGTGT+TGG | 0.485142 | 1_3:+10403634 | Msa0103260:five_prime_UTR |
| GGAGTAAAGAAAAGCTGAAC+CGG | 0.489718 | 1_3:-10403768 | None:intergenic |
| CCGGGTCGGTTCGACCGAAC+CGG | 0.496072 | 1_3:-10403737 | None:intergenic |
| GAGCAGATCCGAAGCTGAGA+AGG | 0.503469 | 1_3:+10403521 | Msa0103260:five_prime_UTR |
| GCTGAACCGGTTCAGGAACC+GGG | 0.538766 | 1_3:-10403755 | None:intergenic |
| TGGGAACAGAGGAATGAAAC+GGG | 0.563195 | 1_3:+10403691 | Msa0103260:five_prime_UTR |
| GTGGGAACAGAGGAATGAAA+CGG | 0.565654 | 1_3:+10403690 | Msa0103260:five_prime_UTR |
| ATGTTGATTGAAATTGGAAG+TGG | 0.577999 | 1_3:-10403314 | None:intergenic |
| ATCCGAAGCTGAGAAGGAAG+AGG | 0.578030 | 1_3:+10403527 | Msa0103260:five_prime_UTR |
| CCGGTTCGGTCGAACCGACC+CGG | 0.579998 | 1_3:+10403737 | Msa0103260:CDS |
| GAGGAGCACGGAAACTGGAT+CGG | 0.581402 | 1_3:+10403452 | Msa0103260:five_prime_UTR |
| TGTTGGTGGCGTGGGAACAG+AGG | 0.609038 | 1_3:+10403680 | Msa0103260:five_prime_UTR |
| CCTTTGGATCGAACAAAAGG+AGG | 0.610982 | 1_3:-10403428 | None:intergenic |
| CGATCCAAAGGAGAGGAGCA+CGG | 0.618840 | 1_3:+10403440 | Msa0103260:five_prime_UTR |
| AACCGGTTCAGGAACCGGGT+CGG | 0.646620 | 1_3:-10403751 | None:intergenic |
| TGGGCCAAGGAGTAAAACTG+TGG | 0.647218 | 1_3:-10403795 | None:intergenic |
| GGGCCAAGGAGTAAAACTGT+GGG | 0.674862 | 1_3:-10403794 | None:intergenic |
| GGAACGAATCGATCTCAGCA+AGG | 0.681192 | 1_3:+10403395 | Msa0103260:five_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAACCAAAGAAAGAAAAA+AGG | - | chr1_3:10403261-10403280 | None:intergenic | 20.0% |
| !!! | ATATAGATGTTGATTGAAAT+TGG | - | chr1_3:10403323-10403342 | None:intergenic | 20.0% |
| !!! | TATATTTTCGAAAATCAACA+GGG | + | chr1_3:10403338-10403357 | Msa0103260:five_prime_UTR | 20.0% |
| !!! | TTGAAGAAAATGAATTTTGT+TGG | + | chr1_3:10403663-10403682 | Msa0103260:five_prime_UTR | 20.0% |
| !! | CTATATTTTCGAAAATCAAC+AGG | + | chr1_3:10403337-10403356 | Msa0103260:five_prime_UTR | 25.0% |
| ! | ACACAAACATTAAGATGAAT+GGG | - | chr1_3:10403575-10403594 | None:intergenic | 25.0% |
| !! | AAGAAAATGAATTTTGTTGG+TGG | + | chr1_3:10403666-10403685 | Msa0103260:five_prime_UTR | 25.0% |
| !!! | CACAACCTTTTTTCTTTCTT+TGG | + | chr1_3:10403253-10403272 | Msa0103260:five_prime_UTR | 30.0% |
| ! | ATGTTGATTGAAATTGGAAG+TGG | - | chr1_3:10403317-10403336 | None:intergenic | 30.0% |
| TAAGATGAATGGGGAAAAAA+AGG | - | chr1_3:10403565-10403584 | None:intergenic | 30.0% | |
| CACAAACATTAAGATGAATG+GGG | - | chr1_3:10403574-10403593 | None:intergenic | 30.0% | |
| CACACAAACATTAAGATGAA+TGG | - | chr1_3:10403576-10403595 | None:intergenic | 30.0% | |
| CATTCATCTTAATGTTTGTG+TGG | + | chr1_3:10403574-10403593 | Msa0103260:intron | 30.0% | |
| !! | GTGGTATTCTTTTTGAAAGT+TGG | + | chr1_3:10403593-10403612 | Msa0103260:intron | 30.0% |
| TGTGAGTTGTTACGTGAAAT+AGG | - | chr1_3:10403237-10403256 | None:intergenic | 35.0% | |
| ATAGAGAGAGAGAAGAGAAT+TGG | + | chr1_3:10403628-10403647 | Msa0103260:intron | 35.0% | |
| !! | TGCTTTTGTATTTGCTGAAC+CGG | + | chr1_3:10403718-10403737 | Msa0103260:CDS | 35.0% |
| ! | TGTGTTGTTATGCTGGATTC+GGG | - | chr1_3:10403284-10403303 | None:intergenic | 40.0% |
| ! | GTGTGTTGTTATGCTGGATT+CGG | - | chr1_3:10403285-10403304 | None:intergenic | 40.0% |
| ! | GCTTATGTGTGTTGTTATGC+TGG | - | chr1_3:10403291-10403310 | None:intergenic | 40.0% |
| TTCGTTCCATCAGTTCGAAA+TGG | - | chr1_3:10403383-10403402 | None:intergenic | 40.0% | |
| TCTCCTTTGGATCGAACAAA+AGG | - | chr1_3:10403434-10403453 | None:intergenic | 40.0% | |
| ! | TTTTGTTCGATCCAAAGGAG+AGG | + | chr1_3:10403433-10403452 | Msa0103260:five_prime_UTR | 40.0% |
| AGAGAGAAGAGAATTGGTGT+TGG | + | chr1_3:10403634-10403653 | Msa0103260:five_prime_UTR | 40.0% | |
| !!! | AATGAATTTTGTTGGTGGCG+TGG | + | chr1_3:10403671-10403690 | Msa0103260:five_prime_UTR | 40.0% |
| !!! | ATGAATTTTGTTGGTGGCGT+GGG | + | chr1_3:10403672-10403691 | Msa0103260:five_prime_UTR | 40.0% |
| ! | TTGTATTTGCTGAACCGGTT+CGG | + | chr1_3:10403723-10403742 | Msa0103260:CDS | 40.0% |
| GGAGTAAAGAAAAGCTGAAC+CGG | - | chr1_3:10403771-10403790 | None:intergenic | 40.0% | |
| CCTTTGGATCGAACAAAAGG+AGG | - | chr1_3:10403431-10403450 | None:intergenic | 45.0% | |
| ! | CCTCCTTTTGTTCGATCCAA+AGG | + | chr1_3:10403428-10403447 | Msa0103260:five_prime_UTR | 45.0% |
| TACCTCTTCCTTCTCAGCTT+CGG | - | chr1_3:10403532-10403551 | None:intergenic | 45.0% | |
| GTGGGAACAGAGGAATGAAA+CGG | + | chr1_3:10403690-10403709 | Msa0103260:five_prime_UTR | 45.0% | |
| TGGGAACAGAGGAATGAAAC+GGG | + | chr1_3:10403691-10403710 | Msa0103260:five_prime_UTR | 45.0% | |
| ! | AAGAAAAGCTGAACCGGTTC+AGG | - | chr1_3:10403765-10403784 | None:intergenic | 45.0% |
| CGCAAGCCATTTCGAACTGA+TGG | + | chr1_3:10403374-10403393 | Msa0103260:five_prime_UTR | 50.0% | |
| GGAACGAATCGATCTCAGCA+AGG | + | chr1_3:10403395-10403414 | Msa0103260:five_prime_UTR | 50.0% | |
| ATCCGAAGCTGAGAAGGAAG+AGG | + | chr1_3:10403527-10403546 | Msa0103260:five_prime_UTR | 50.0% | |
| CGATCCAAAGGAGAGGAGCA+CGG | + | chr1_3:10403440-10403459 | Msa0103260:five_prime_UTR | 55.0% | |
| GTTTCCGTGCTCCTCTCCTT+TGG | - | chr1_3:10403447-10403466 | None:intergenic | 55.0% | |
| ! | AAGGAGAGGAGCACGGAAAC+TGG | + | chr1_3:10403447-10403466 | Msa0103260:five_prime_UTR | 55.0% |
| ! | GAGGAGCACGGAAACTGGAT+CGG | + | chr1_3:10403452-10403471 | Msa0103260:five_prime_UTR | 55.0% |
| GAGCAGATCCGAAGCTGAGA+AGG | + | chr1_3:10403521-10403540 | Msa0103260:five_prime_UTR | 55.0% | |
| ! | AGCTGAACCGGTTCAGGAAC+CGG | - | chr1_3:10403759-10403778 | None:intergenic | 55.0% |
| !! | TGTTGGTGGCGTGGGAACAG+AGG | + | chr1_3:10403680-10403699 | Msa0103260:five_prime_UTR | 60.0% |
| AACCGACCCGGTTCCTGAAC+CGG | + | chr1_3:10403749-10403768 | Msa0103260:CDS | 60.0% | |
| ! | AACCGGTTCAGGAACCGGGT+CGG | - | chr1_3:10403754-10403773 | None:intergenic | 60.0% |
| ! | GCTGAACCGGTTCAGGAACC+GGG | - | chr1_3:10403758-10403777 | None:intergenic | 60.0% |
| !! | CCGGGTCGGTTCGACCGAAC+CGG | - | chr1_3:10403740-10403759 | None:intergenic | 70.0% |
| !! | CCGGTTCGGTCGAACCGACC+CGG | + | chr1_3:10403737-10403756 | Msa0103260:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_3 | gene | 10403232 | 10403803 | 10403232 | ID=Msa0103260;Name=Msa0103260 |
| chr1_3 | mRNA | 10403232 | 10403803 | 10403232 | ID=Msa0103260-mRNA-1;Parent=Msa0103260;Name=Msa0103260-mRNA-1;_AED=0.00;_eAED=0.00;_QI=402|1|0.5|1|0|0.5|2|0|29 |
| chr1_3 | exon | 10403232 | 10403548 | 10403232 | ID=Msa0103260-mRNA-1:exon:4319;Parent=Msa0103260-mRNA-1 |
| chr1_3 | exon | 10403632 | 10403803 | 10403632 | ID=Msa0103260-mRNA-1:exon:4320;Parent=Msa0103260-mRNA-1 |
| chr1_3 | five_prime_UTR | 10403232 | 10403548 | 10403232 | ID=Msa0103260-mRNA-1:five_prime_utr;Parent=Msa0103260-mRNA-1 |
| chr1_3 | five_prime_UTR | 10403632 | 10403716 | 10403632 | ID=Msa0103260-mRNA-1:five_prime_utr;Parent=Msa0103260-mRNA-1 |
| chr1_3 | CDS | 10403717 | 10403803 | 10403717 | ID=Msa0103260-mRNA-1:cds;Parent=Msa0103260-mRNA-1 |
| Gene Sequence |
| Protein sequence |
