Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0103260 | Msa0347230 | 0.881320 | 2.647586e-70 | -8.615850e-47 |
| Msa0106710 | Msa0347230 | 0.800735 | 1.253931e-48 | -8.615850e-47 |
| Msa0347230 | Msa0526020 | 0.803734 | 3.026014e-49 | -8.615850e-47 |
| Msa0347230 | Msa0581400 | 0.810960 | 8.888489e-51 | -8.615850e-47 |
| Msa0347230 | Msa0616650 | 0.807074 | 6.036299e-50 | -8.615850e-47 |
| Msa0347230 | Msa0624060 | 0.801519 | 8.667019e-49 | -8.615850e-47 |
| Msa0347230 | Msa0721280 | 0.800478 | 1.414304e-48 | -8.615850e-47 |
| Msa0347230 | Msa0756380 | 0.801307 | 9.575603e-49 | -8.615850e-47 |
| Msa0347230 | Msa1124160 | 0.858006 | 1.107716e-62 | -8.615850e-47 |
| Msa0347230 | Msa1378410 | 0.804252 | 2.361196e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATATAGATGTTGATTGAAAT+TGG | 0.232139 | 3_1:+8188433 | None:intergenic |
| TGTGAGTTGTTACGTGAAAT+AGG | 0.334697 | 3_1:+8188519 | None:intergenic |
| AAGGAGAGGAGCACGGAAAC+TGG | 0.341508 | 3_1:-8188306 | Msa0347230:five_prime_UTR |
| GTTTCCGTGCTCCTCTCCTT+TGG | 0.345017 | 3_1:+8188309 | None:intergenic |
| GTGTGTTGTTATGCTGGATT+CGG | 0.357976 | 3_1:+8188471 | None:intergenic |
| AGCTGAACCGGTTCAGGAAC+CGG | 0.361281 | 3_1:+8187997 | None:intergenic |
| AAGAAAAGCTGAACCGGTTC+AGG | 0.362146 | 3_1:+8187991 | None:intergenic |
| TACCTCTTCCTTCTCAGCTT+CGG | 0.371599 | 3_1:+8188224 | None:intergenic |
| TTGTATTTGCTGAACCGGTT+CGG | 0.381246 | 3_1:-8188030 | Msa0347230:CDS |
| ATAGAGAGAGAGAAGAGAAT+TGG | 0.384503 | 3_1:-8188125 | Msa0347230:intron |
| AACCGACCCGGTTCCTGAAC+CGG | 0.403375 | 3_1:-8188004 | Msa0347230:CDS |
| TGTGTTGTTATGCTGGATTC+GGG | 0.411143 | 3_1:+8188472 | None:intergenic |
| GCTTATGTGTGTTGTTATGC+TGG | 0.414463 | 3_1:+8188465 | None:intergenic |
| AAGGAGTAAAACTGTGGGCT+TGG | 0.438906 | 3_1:+8187964 | None:intergenic |
| TTCGTTCCATCAGTTCGAAA+TGG | 0.443647 | 3_1:+8188373 | None:intergenic |
| CGCAAGCCATTTCGAACTGA+TGG | 0.458753 | 3_1:-8188379 | Msa0347230:five_prime_UTR |
| TCTCCTTTGGATCGAACAAA+AGG | 0.474957 | 3_1:+8188322 | None:intergenic |
| AGAGAGAAGAGAATTGGTGT+TGG | 0.485142 | 3_1:-8188119 | Msa0347230:five_prime_UTR |
| GGAGTAAAGAAAAGCTGAAC+CGG | 0.489718 | 3_1:+8187985 | None:intergenic |
| CCGGGTCGGTTCGACCGAAC+CGG | 0.496072 | 3_1:+8188016 | None:intergenic |
| GAGCAGATCCGAAGCTGAGA+AGG | 0.503469 | 3_1:-8188232 | Msa0347230:five_prime_UTR |
| GCTGAACCGGTTCAGGAACC+GGG | 0.538766 | 3_1:+8187998 | None:intergenic |
| TGGGAACAGAGGAATGAAAC+GGG | 0.563195 | 3_1:-8188062 | Msa0347230:five_prime_UTR |
| GTGGGAACAGAGGAATGAAA+CGG | 0.565654 | 3_1:-8188063 | Msa0347230:five_prime_UTR |
| ATGTTGATTGAAATTGGAAG+TGG | 0.577999 | 3_1:+8188439 | None:intergenic |
| ATCCGAAGCTGAGAAGGAAG+AGG | 0.578030 | 3_1:-8188226 | Msa0347230:intron |
| CCGGTTCGGTCGAACCGACC+CGG | 0.579998 | 3_1:-8188016 | Msa0347230:CDS |
| GAGGAGCACGGAAACTGGAT+CGG | 0.581402 | 3_1:-8188301 | Msa0347230:five_prime_UTR |
| TGTTGGTGGCGTGGGAACAG+AGG | 0.609038 | 3_1:-8188073 | Msa0347230:five_prime_UTR |
| CCTTTGGATCGAACAAAAGG+AGG | 0.610982 | 3_1:+8188325 | None:intergenic |
| CGATCCAAAGGAGAGGAGCA+CGG | 0.618840 | 3_1:-8188313 | Msa0347230:five_prime_UTR |
| AACCGGTTCAGGAACCGGGT+CGG | 0.646620 | 3_1:+8188002 | None:intergenic |
| TGGGCCAAGGAGTAAAACTG+TGG | 0.647218 | 3_1:+8187958 | None:intergenic |
| GGGCCAAGGAGTAAAACTGT+GGG | 0.674862 | 3_1:+8187959 | None:intergenic |
| GGAACGAATCGATCTCAGCA+AGG | 0.681192 | 3_1:-8188358 | Msa0347230:five_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAACCAAAGAAAGAAAAA+AGG | + | chr3_1:8188001-8188020 | None:intergenic | 20.0% |
| !!! | ATATAGATGTTGATTGAAAT+TGG | + | chr3_1:8188063-8188082 | None:intergenic | 20.0% |
| !!! | TATATTTTCGAAAATCAACA+GGG | - | chr3_1:8188078-8188097 | Msa0347230:five_prime_UTR | 20.0% |
| !!! | TTGAAGAAAATGAATTTTGT+TGG | - | chr3_1:8188403-8188422 | Msa0347230:five_prime_UTR | 20.0% |
| !! | CTATATTTTCGAAAATCAAC+AGG | - | chr3_1:8188077-8188096 | Msa0347230:five_prime_UTR | 25.0% |
| ! | ACACAAACATTAAGATGAAT+GGG | + | chr3_1:8188315-8188334 | None:intergenic | 25.0% |
| !! | AAGAAAATGAATTTTGTTGG+TGG | - | chr3_1:8188406-8188425 | Msa0347230:five_prime_UTR | 25.0% |
| ! | ATGTTGATTGAAATTGGAAG+TGG | + | chr3_1:8188057-8188076 | None:intergenic | 30.0% |
| TAAGATGAATGGGGAAAAAA+AGG | + | chr3_1:8188305-8188324 | None:intergenic | 30.0% | |
| CACAAACATTAAGATGAATG+GGG | + | chr3_1:8188314-8188333 | None:intergenic | 30.0% | |
| CACACAAACATTAAGATGAA+TGG | + | chr3_1:8188316-8188335 | None:intergenic | 30.0% | |
| CATTCATCTTAATGTTTGTG+TGG | - | chr3_1:8188314-8188333 | Msa0347230:five_prime_UTR | 30.0% | |
| !! | GTGGTATTCTTTTTGAAAGT+TGG | - | chr3_1:8188333-8188352 | Msa0347230:five_prime_UTR | 30.0% |
| TGTGAGTTGTTACGTGAAAT+AGG | + | chr3_1:8187977-8187996 | None:intergenic | 35.0% | |
| !!! | CACAGCCTTTTTTCTTTCTT+TGG | - | chr3_1:8187993-8188012 | Msa0347230:CDS | 35.0% |
| ATAGAGAGAGAGAAGAGAAT+TGG | - | chr3_1:8188368-8188387 | Msa0347230:five_prime_UTR | 35.0% | |
| !! | TGCTTTTGTATTTGCTGAAC+CGG | - | chr3_1:8188458-8188477 | Msa0347230:five_prime_UTR | 35.0% |
| ! | TGTGTTGTTATGCTGGATTC+GGG | + | chr3_1:8188024-8188043 | None:intergenic | 40.0% |
| ! | GTGTGTTGTTATGCTGGATT+CGG | + | chr3_1:8188025-8188044 | None:intergenic | 40.0% |
| ! | GCTTATGTGTGTTGTTATGC+TGG | + | chr3_1:8188031-8188050 | None:intergenic | 40.0% |
| TTCGTTCCATCAGTTCGAAA+TGG | + | chr3_1:8188123-8188142 | None:intergenic | 40.0% | |
| TCTCCTTTGGATCGAACAAA+AGG | + | chr3_1:8188174-8188193 | None:intergenic | 40.0% | |
| ! | TTTTGTTCGATCCAAAGGAG+AGG | - | chr3_1:8188173-8188192 | Msa0347230:intron | 40.0% |
| AGAGAGAAGAGAATTGGTGT+TGG | - | chr3_1:8188374-8188393 | Msa0347230:five_prime_UTR | 40.0% | |
| !!! | AATGAATTTTGTTGGTGGCG+TGG | - | chr3_1:8188411-8188430 | Msa0347230:five_prime_UTR | 40.0% |
| !!! | ATGAATTTTGTTGGTGGCGT+GGG | - | chr3_1:8188412-8188431 | Msa0347230:five_prime_UTR | 40.0% |
| ! | TTGTATTTGCTGAACCGGTT+CGG | - | chr3_1:8188463-8188482 | Msa0347230:five_prime_UTR | 40.0% |
| GGAGTAAAGAAAAGCTGAAC+CGG | + | chr3_1:8188511-8188530 | None:intergenic | 40.0% | |
| CCTTTGGATCGAACAAAAGG+AGG | + | chr3_1:8188171-8188190 | None:intergenic | 45.0% | |
| ! | CCTCCTTTTGTTCGATCCAA+AGG | - | chr3_1:8188168-8188187 | Msa0347230:intron | 45.0% |
| TACCTCTTCCTTCTCAGCTT+CGG | + | chr3_1:8188272-8188291 | None:intergenic | 45.0% | |
| GTGGGAACAGAGGAATGAAA+CGG | - | chr3_1:8188430-8188449 | Msa0347230:five_prime_UTR | 45.0% | |
| TGGGAACAGAGGAATGAAAC+GGG | - | chr3_1:8188431-8188450 | Msa0347230:five_prime_UTR | 45.0% | |
| ! | AAGAAAAGCTGAACCGGTTC+AGG | + | chr3_1:8188505-8188524 | None:intergenic | 45.0% |
| CGCAAGCCATTTCGAACTGA+TGG | - | chr3_1:8188114-8188133 | Msa0347230:five_prime_UTR | 50.0% | |
| GGAACGAATCGATCTCAGCA+AGG | - | chr3_1:8188135-8188154 | Msa0347230:intron | 50.0% | |
| ATCCGAAGCTGAGAAGGAAG+AGG | - | chr3_1:8188267-8188286 | Msa0347230:five_prime_UTR | 50.0% | |
| CGATCCAAAGGAGAGGAGCA+CGG | - | chr3_1:8188180-8188199 | Msa0347230:intron | 55.0% | |
| GTTTCCGTGCTCCTCTCCTT+TGG | + | chr3_1:8188187-8188206 | None:intergenic | 55.0% | |
| ! | AAGGAGAGGAGCACGGAAAC+TGG | - | chr3_1:8188187-8188206 | Msa0347230:intron | 55.0% |
| ! | GAGGAGCACGGAAACTGGAT+CGG | - | chr3_1:8188192-8188211 | Msa0347230:intron | 55.0% |
| GAGCAGATCCGAAGCTGAGA+AGG | - | chr3_1:8188261-8188280 | Msa0347230:five_prime_UTR | 55.0% | |
| ! | AGCTGAACCGGTTCAGGAAC+CGG | + | chr3_1:8188499-8188518 | None:intergenic | 55.0% |
| !! | TGTTGGTGGCGTGGGAACAG+AGG | - | chr3_1:8188420-8188439 | Msa0347230:five_prime_UTR | 60.0% |
| AACCGACCCGGTTCCTGAAC+CGG | - | chr3_1:8188489-8188508 | Msa0347230:five_prime_UTR | 60.0% | |
| ! | AACCGGTTCAGGAACCGGGT+CGG | + | chr3_1:8188494-8188513 | None:intergenic | 60.0% |
| ! | GCTGAACCGGTTCAGGAACC+GGG | + | chr3_1:8188498-8188517 | None:intergenic | 60.0% |
| !! | CCGGGTCGGTTCGACCGAAC+CGG | + | chr3_1:8188480-8188499 | None:intergenic | 70.0% |
| !! | CCGGTTCGGTCGAACCGACC+CGG | - | chr3_1:8188477-8188496 | Msa0347230:five_prime_UTR | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_1 | gene | 8187972 | 8188543 | 8187972 | ID=Msa0347230;Name=Msa0347230 |
| chr3_1 | mRNA | 8187972 | 8188543 | 8187972 | ID=Msa0347230-mRNA-1;Parent=Msa0347230;Name=Msa0347230-mRNA-1;_AED=0.00;_eAED=0.00;_QI=402|1|0.5|1|1|1|2|0|29 |
| chr3_1 | exon | 8187972 | 8188143 | 8187972 | ID=Msa0347230-mRNA-1:exon:2358;Parent=Msa0347230-mRNA-1 |
| chr3_1 | exon | 8188227 | 8188543 | 8188227 | ID=Msa0347230-mRNA-1:exon:2357;Parent=Msa0347230-mRNA-1 |
| chr3_1 | five_prime_UTR | 8188227 | 8188543 | 8188227 | ID=Msa0347230-mRNA-1:five_prime_utr;Parent=Msa0347230-mRNA-1 |
| chr3_1 | five_prime_UTR | 8188059 | 8188143 | 8188059 | ID=Msa0347230-mRNA-1:five_prime_utr;Parent=Msa0347230-mRNA-1 |
| chr3_1 | CDS | 8187972 | 8188058 | 8187972 | ID=Msa0347230-mRNA-1:cds;Parent=Msa0347230-mRNA-1 |
| Gene Sequence |
| Protein sequence |
