AlfalfaGEDB | Msa0105630

M. sativa cultivar zhongmu-4 / Msa0105630

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0105630	RHN78016.1	81.381	333	20	2	1	291	985	1317	1.02e-162	505

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0105630	A0A396JIG2	81.381	333	20	2	1	291	985	1317	4.87e-163	505

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0105630	Msa0152230	0.805728	1.160326e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0105630	MtrunA17_Chr1g0160951	81.381	333	20	2	1	291	985	1317	9.38e-167	505
Msa0105630	MtrunA17_Chr3g0107931	29.836	305	168	9	1	266	1215	1512	1.60e-28	115
Msa0105630	MtrunA17_Chr3g0108111	27.338	278	156	8	3	240	140	411	4.11e-24	102
Msa0105630	MtrunA17_Chr3g0091861	27.476	313	178	8	3	273	1139	1444	4.93e-24	102
Msa0105630	MtrunA17_Chr5g0439121	34.979	243	102	10	32	227	1299	1532	1.11e-23	101
Msa0105630	MtrunA17_Chr3g0097601	28.311	219	120	7	21	203	14	231	3.62e-21	90.5
Msa0105630	MtrunA17_Chr3g0108011	27.551	294	177	8	3	269	1226	1510	2.10e-20	91.7
Msa0105630	MtrunA17_Chr3g0107271	31.507	219	116	5	21	206	13	230	3.31e-20	87.4
Msa0105630	MtrunA17_Chr3g0108171	29.565	230	129	8	69	274	1204	1424	6.99e-20	90.1
Msa0105630	MtrunA17_Chr3g0108071	28.235	255	135	8	15	230	351	596	1.63e-19	88.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 52 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGACAATGAGGAAAATCAA+AGG	0.619508	1_3:+14222694	Msa0105630:CDS
AAGCATATCAGTTGAGATTG+AGG	0.540937	1_3:-14222726	None:intergenic
AAGGGAGCTTCCTTGTTCAC+AGG	0.501430	1_3:+14223417	Msa0105630:CDS
ACAGGAAGTTCAAGATGTTC+AGG	0.365825	1_3:+14223378	Msa0105630:CDS
ACATTGATCGCGATGTTCAC+TGG	0.419850	1_3:+14223269	Msa0105630:CDS
ACTGGCCAACACATCCACTG+AGG	0.764018	1_3:+14221993	Msa0105630:CDS
AGATTCCTAATCAGAAACTT+CGG	0.565853	1_3:+14222901	Msa0105630:CDS
AGCACTAGATATTTGAGTTT+AGG	0.345136	1_3:-14222927	None:intergenic
AGGTTCAATCAGCATCTCAA+AGG	0.554460	1_3:+14223398	Msa0105630:CDS
AGTGATTTCATGTTTCATTT+GGG	0.327091	1_3:+14221961	Msa0105630:exon

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGATGGTTAATTACGTTCAA+TGG	-	chr1_3:14222152-14222171	None:intergenic	30.0%
AGTGTATGCTTCAATTCTAT+AGG	+	chr1_3:14222341-14222360	Msa0105630:intron	30.0%
TGAACAATCTACATGATCTA+AGG	+	chr1_3:14222502-14222521	Msa0105630:CDS	30.0%
AAGACAATGAGGAAAATCAA+AGG	+	chr1_3:14222694-14222713	Msa0105630:CDS	30.0%
AGATTCCTAATCAGAAACTT+CGG	+	chr1_3:14222901-14222920	Msa0105630:CDS	30.0%
GTTGAATCAAATGAAAGGTT+AGG	-	chr1_3:14223032-14223051	None:intergenic	30.0%
TGATGAGTGGTTTCTAATAT+TGG	-	chr1_3:14223346-14223365	None:intergenic	30.0%
TTAGTATTAACCTGTGAACA+AGG	-	chr1_3:14223430-14223449	None:intergenic	30.0%
TGATGGTTAATTACGTTCAA+TGG	-	chr1_3:14222152-14222171	None:intergenic	30.0%
AGTGTATGCTTCAATTCTAT+AGG	+	chr1_3:14222341-14222360	Msa0105630:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	14221961	14223615	14221961	ID=Msa0105630;Name=Msa0105630
chr1_3	mRNA	14221961	14223615	14221961	ID=Msa0105630-mRNA-1;Parent=Msa0105630;Name=Msa0105630-mRNA-1;_AED=0.38;_eAED=0.38;_QI=9\|0.2\|0.33\|0.33\|0.6\|0.33\|6\|0\|293
chr1_3	exon	14221961	14222014	14221961	ID=Msa0105630-mRNA-1:exon:5613;Parent=Msa0105630-mRNA-1
chr1_3	exon	14222363	14222523	14222363	ID=Msa0105630-mRNA-1:exon:5614;Parent=Msa0105630-mRNA-1
chr1_3	exon	14222596	14222832	14222596	ID=Msa0105630-mRNA-1:exon:5615;Parent=Msa0105630-mRNA-1
chr1_3	exon	14222887	14223086	14222887	ID=Msa0105630-mRNA-1:exon:5616;Parent=Msa0105630-mRNA-1
chr1_3	exon	14223263	14223438	14223263	ID=Msa0105630-mRNA-1:exon:5617;Parent=Msa0105630-mRNA-1
chr1_3	exon	14223553	14223615	14223553	ID=Msa0105630-mRNA-1:exon:5618;Parent=Msa0105630-mRNA-1
chr1_3	five_prime_UTR	14221961	14221969	14221961	ID=Msa0105630-mRNA-1:five_prime_utr;Parent=Msa0105630-mRNA-1
chr1_3	CDS	14221970	14222014	14221970	ID=Msa0105630-mRNA-1:cds;Parent=Msa0105630-mRNA-1
chr1_3	CDS	14222363	14222523	14222363	ID=Msa0105630-mRNA-1:cds;Parent=Msa0105630-mRNA-1
chr1_3	CDS	14222596	14222832	14222596	ID=Msa0105630-mRNA-1:cds;Parent=Msa0105630-mRNA-1
chr1_3	CDS	14222887	14223086	14222887	ID=Msa0105630-mRNA-1:cds;Parent=Msa0105630-mRNA-1
chr1_3	CDS	14223263	14223438	14223263	ID=Msa0105630-mRNA-1:cds;Parent=Msa0105630-mRNA-1
chr1_3	CDS	14223553	14223615	14223553	ID=Msa0105630-mRNA-1:cds;Parent=Msa0105630-mRNA-1

Gene Sequence

>Msa0105630

ATGTTTCATTTGGGTGCAAGACAACTGGCCAACACATCCACTGAGGACATAGGAGAAATCATGAAGCATTTCCAGACTCTAGAAAAGCTATGCATAGAGAACTCAGACATTCAAGGGATATTTGGTCTTGAAGCACATCCAATCATTGGTCAACAAATGAGCTCAAGTTTGCAATCCTTGAAATTGAACAATCTACATGATCTAAGTAAACTCAAAGTTGTCTTCTCAGCATCTGTCTTGAGAAGCCTGCCACAACTTGCATATCTAGAAATCAGCAACTGTGAAGGATTACATAAAATAATGGAAGACAATGAGGAAAATCAAAGGCAGACAATTCCTCAATCTCAACTGATATGCTTTCCGAAGCTCGTTGCACTTATCATCAAGAGTTGCCACAGTTTGAAAAGTCTCATCTCTGTCACCACATTCCAAGAGTTTCCAAATGAACCACATGATGAGATTCCTAATCAGAAACTTCGGCTTCCTAAACTCAAATATCTAGTGCTTATGCAACTGCCGAACCTCACTGATCCGAGCCAACATATGGAGTTGCAGAATGTGACATACAGCATTGTGGATCATTGCCCTAACCTTTCATTTGATTCAACAACTACTCTTGAGGACTTAAAGAATATATTAGAAGATAGTAACATTGATCGCGATGTTCACTGGGAGTTATTTGAAATATTTGACACTATAGTGGAAGAGGCTGAAAATGAAAATCCAATATTAGAAACCACTCATCAATTACCGATAGTACAGGAAGTTCAAGATGTTCAGGTTCAATCAGCATCTCAAAGGGAGCTTCCTTGTTCACAGATTATTGATGAAGCAGATAACAAAATTGTTGCTGTACATGATTCAAGAATGGAACAGATTTAG

Protein sequence

>Msa0105630

MFHLGARQLANTSTEDIGEIMKHFQTLEKLCIENSDIQGIFGLEAHPIIGQQMSSSLQSLKLNNLHDLSKLKVVFSASVLRSLPQLAYLEISNCEGLHKIMEDNEENQRQTIPQSQLICFPKLVALIIKSCHSLKSLISVTTFQEFPNEPHDEIPNQKLRLPKLKYLVLMQLPNLTDPSQHMELQNVTYSIVDHCPNLSFDSTTTLEDLKNILEDSNIDRDVHWELFEIFDTIVEEAENENPILETTHQLPIVQEVQDVQVQSASQRELPCSQIIDEADNKIVAVHDSRMEQI*