AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0113010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 54 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCATTTGAGCTTTGAAATA+AGG 0.154303 1_3:-28675803 Msa0113010:three_prime_UTR
ATAACAAATTTAATTTAATA+TGG 0.185007 1_3:-28676128 Msa0113010:CDS
TGAGCATGGCATTAACTTTC+TGG 0.185498 1_3:+28679694 None:intergenic
TGTAGAGATTAGTAGAAGAT+AGG 0.251868 1_3:+28679770 None:intergenic
GTTCTCTTAAGCATGGAATT+AGG 0.287198 1_3:-28675765 Msa0113010:three_prime_UTR
TCAATTTCAAGCTACTTCTA+AGG 0.290144 1_3:-28676742 Msa0113010:CDS
TGTCTATTGCATGGTCTTTA+CGG 0.300319 1_3:-28675552 Msa0113010:three_prime_UTR
TGTATTCTGCACTTTGCTAT+TGG 0.313600 1_3:-28675927 Msa0113010:three_prime_UTR
TTAATTCATAGTAAGACTAT+TGG 0.329902 1_3:-28676795 Msa0113010:CDS
TTAATTTAATATGGATATGA+TGG 0.345164 1_3:-28676119 Msa0113010:exon
AATGAAGGAAGAAGATAGAT+AGG 0.355035 1_3:+28679820 None:intergenic
GTAGAGATTAGTAGAAGATA+GGG 0.389518 1_3:+28679771 None:intergenic
TCAACTACCTTCACATGATA+AGG 0.413493 1_3:-28676829 Msa0113010:CDS
CAACGCTAGTGGAGTTGAAA+AGG 0.435330 1_3:+28679654 None:intergenic
AGTAGAAGATAGGGGAAAAG+AGG 0.441306 1_3:+28679780 None:intergenic
TAAGGACAACCGAAGGAGTA+AGG 0.449016 1_3:+28679720 None:intergenic
GGAAAAGAGGATCAGAAGGC+AGG 0.449989 1_3:+28679793 None:intergenic
TTTGGATATTGTCTATTGCA+TGG 0.471486 1_3:-28675561 Msa0113010:three_prime_UTR
ACTGCCACCCCTTACTCCTT+CGG 0.475857 1_3:-28679729 Msa0113010:five_prime_UTR
TCTGTAATCACCTCATTCAT+TGG 0.482922 1_3:+28679572 None:intergenic
AGAGAAAGAGAGGAAGATAG+AGG 0.488441 1_3:+28679853 None:intergenic
AACGCTAGTGGAGTTGAAAA+GGG 0.493587 1_3:+28679655 None:intergenic
GCATTAACTTTCTGGAGGTA+AGG 0.497772 1_3:+28679702 None:intergenic
TTACTCTTCTCCAATGAATG+AGG 0.500422 1_3:-28679582 Msa0113010:five_prime_UTR
AACAAAATTCAGAGAAAGAG+AGG 0.514132 1_3:+28679843 None:intergenic
ATGTTTGAGTAGGAAGAATG+AGG 0.515310 1_3:-28675831 Msa0113010:intron
GAAAGAGAGGAAGATAGAGG+AGG 0.519024 1_3:+28679856 None:intergenic
TATGCGATGCACAATTATAG+AGG 0.528147 1_3:-28675640 Msa0113010:three_prime_UTR
TTCTCATGAGAACAAGCAAA+AGG 0.529960 1_3:-28679423 Msa0113010:intron
GTCTACAACTTTGACATGAC+AGG 0.532540 1_3:+28676769 None:intergenic
CTCGATGGTCGGTCATAGCG+AGG 0.541157 1_3:-28679629 Msa0113010:five_prime_UTR
GAAAAGAGGATCAGAAGGCA+GGG 0.544812 1_3:+28679794 None:intergenic
TGATGATGTTCTCTTAAGCA+TGG 0.545293 1_3:-28675772 Msa0113010:three_prime_UTR
AAGGACAACCGAAGGAGTAA+GGG 0.557009 1_3:+28679721 None:intergenic
CAAAATGTAAAAGAATCTAA+AGG 0.559695 1_3:+28679548 None:intergenic
ATGCGATGCACAATTATAGA+GGG 0.570501 1_3:-28675639 Msa0113010:three_prime_UTR
TCCACTAGCGTTGAACTCGA+TGG 0.571047 1_3:-28679644 Msa0113010:five_prime_UTR
CAGAAGGCAGGGGATAATGA+AGG 0.574086 1_3:+28679805 None:intergenic
TAGAGATTAGTAGAAGATAG+GGG 0.580074 1_3:+28679772 None:intergenic
TAGGGGAAAAGAGGATCAGA+AGG 0.592103 1_3:+28679789 None:intergenic
ACCATCGAGTTCAACGCTAG+TGG 0.593922 1_3:+28679643 None:intergenic
CTATTGCATGGTCTTTACGG+TGG 0.599039 1_3:-28675549 Msa0113010:three_prime_UTR
TACCTTGGACTTGAAAATGA+TGG 0.619298 1_3:+28676717 None:intergenic
GAAACATAAGAGTATGAGCA+TGG 0.621463 1_3:+28679680 None:intergenic
AGGACAACCGAAGGAGTAAG+GGG 0.630369 1_3:+28679722 None:intergenic
AAAAGAGGATCAGAAGGCAG+GGG 0.644997 1_3:+28679795 None:intergenic
ACGCTAGTGGAGTTGAAAAG+GGG 0.650616 1_3:+28679656 None:intergenic
GCATGGCATTAACTTTCTGG+AGG 0.659849 1_3:+28679697 None:intergenic
TCGATGGTCGGTCATAGCGA+GGG 0.660332 1_3:-28679628 Msa0113010:five_prime_UTR
CTAGCGTTGAACTCGATGGT+CGG 0.672884 1_3:-28679640 Msa0113010:five_prime_UTR
CTGGAGGTAAGGACAACCGA+AGG 0.688387 1_3:+28679713 None:intergenic
AATATGGATATGATGGACAG+TGG 0.692254 1_3:-28676112 Msa0113010:exon
ACAACCGAAGGAGTAAGGGG+TGG 0.701061 1_3:+28679725 None:intergenic
GGAATCTAATACGATACACA+AGG 0.732293 1_3:-28679607 Msa0113010:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAACTAAAAATGATATA+TGG + chr1_3:28676147-28676166 None:intergenic 10.0%
!!! TATATGATTTTAATTTCTTT+TGG - chr1_3:28678212-28678231 Msa0113010:intron 10.0%
!!! ATATGATTTTAATTTCTTTT+GGG - chr1_3:28678213-28678232 Msa0113010:intron 10.0%
!!! AAATTTTCTTAAACTTAAAA+GGG + chr1_3:28679684-28679703 None:intergenic 10.0%
!!! AAAATTTTCTTAAACTTAAA+AGG + chr1_3:28679685-28679704 None:intergenic 10.0%
!! TTGAAACAAAAAAAAAAACA+TGG + chr1_3:28675949-28675968 None:intergenic 15.0%
!!! TTTTTTTTTTGTTTCAACAA+TGG - chr1_3:28675952-28675971 Msa0113010:three_prime_UTR 15.0%
!!! TTTTTTTGATTAAATAGTAG+AGG - chr1_3:28676239-28676258 Msa0113010:intron 15.0%
!! ATGAAATTACAATTGTTAAA+AGG + chr1_3:28676333-28676352 None:intergenic 15.0%
!!! TAAATTGTTGTAGTTTTTTT+AGG - chr1_3:28676757-28676776 Msa0113010:CDS 15.0%
!!! AAATTGTTGTAGTTTTTTTA+GGG - chr1_3:28676758-28676777 Msa0113010:CDS 15.0%
!!! ATACATTTTAAGATGATTTA+TGG - chr1_3:28677049-28677068 Msa0113010:intron 15.0%
!!! TACATTTTAAGATGATTTAT+GGG - chr1_3:28677050-28677069 Msa0113010:intron 15.0%
!! TAAAATAAAAAATGCACAAA+AGG + chr1_3:28677148-28677167 None:intergenic 15.0%
!!! TTCTTATAACATTATTTTCA+TGG + chr1_3:28677347-28677366 None:intergenic 15.0%
!!! ATGCTGATTTAATTAAATTT+TGG - chr1_3:28677732-28677751 Msa0113010:intron 15.0%
!!! TGCTGATTTAATTAAATTTT+GGG - chr1_3:28677733-28677752 Msa0113010:intron 15.0%
!!! TATTTTTTATATGCATTTCA+CGG - chr1_3:28678044-28678063 Msa0113010:intron 15.0%
!! GAGATAATATCAAAAAATTA+AGG + chr1_3:28678976-28678995 None:intergenic 15.0%
!! TTACAAAATTTAACTAACTA+AGG + chr1_3:28678998-28679017 None:intergenic 15.0%
!! TTAATTTAATATGGATATGA+TGG - chr1_3:28679283-28679302 Msa0113010:intron 15.0%
!!! AAACAATGTTGTTAATTTTT+TGG - chr1_3:28679396-28679415 Msa0113010:intron 15.0%
!!! TGATATTTGATGTAATTTTT+TGG - chr1_3:28679823-28679842 Msa0113010:five_prime_UTR 15.0%
!! CAAAATGTAAAAGAATCTAA+AGG + chr1_3:28675857-28675876 None:intergenic 20.0%
!!! CTTTAGATTCTTTTACATTT+TGG - chr1_3:28675855-28675874 Msa0113010:intron 20.0%
!! GAGTAATATGAATTAGAAAT+GGG + chr1_3:28676125-28676144 None:intergenic 20.0%
!!! TCAATTTTTCTGCATTTTTT+GGG + chr1_3:28676506-28676525 None:intergenic 20.0%
!!! ATAAAAGGTTTGTTGAAATA+TGG - chr1_3:28676645-28676664 Msa0113010:intron 20.0%
!! TACAATAAAAAGAAAAACCA+TGG - chr1_3:28676939-28676958 Msa0113010:intron 20.0%
!!! TTTGATTTCTCATTTTACTA+TGG - chr1_3:28677173-28677192 Msa0113010:intron 20.0%
!!! TACTTTTACTAAATAGAGTT+TGG - chr1_3:28677414-28677433 Msa0113010:intron 20.0%
!! AATCAACTTCTAGATAAAAA+AGG + chr1_3:28677522-28677541 None:intergenic 20.0%
!! AAACTTTATCAAGAGATTTA+TGG + chr1_3:28677781-28677800 None:intergenic 20.0%
!!! TCATTTAAAAGTTTTAGCTA+TGG + chr1_3:28677921-28677940 None:intergenic 20.0%
!!! CTAAAACTTTTAAATGATTG+AGG - chr1_3:28677924-28677943 Msa0113010:intron 20.0%
!! GTGAAATGCATATAAAAAAT+AGG + chr1_3:28678045-28678064 None:intergenic 20.0%
!!! ATTTTTTATATGCATTTCAC+GGG - chr1_3:28678045-28678064 Msa0113010:intron 20.0%
!!! AGTTGTCAATAAAATTTTGA+GGG + chr1_3:28678094-28678113 None:intergenic 20.0%
!! TTAATTCATAGTAAGACTAT+TGG - chr1_3:28678607-28678626 Msa0113010:intron 20.0%
!! TAGTTAGATTGTGTTATATA+TGG - chr1_3:28678858-28678877 Msa0113010:intron 20.0%
!! AGATGAATAAATTATTGAAG+TGG + chr1_3:28679145-28679164 None:intergenic 20.0%
!!! TTCCATTTTCTAATTTGTAT+TGG - chr1_3:28679543-28679562 Msa0113010:five_prime_UTR 20.0%
!! AACCAATACAAATTAGAAAA+TGG + chr1_3:28679548-28679567 None:intergenic 20.0%
!!! TTACATTTTGGAAAGCATTT+TGG - chr1_3:28675867-28675886 Msa0113010:intron 25.0%
! GAAAAAACTTATTCACAAAG+AGG + chr1_3:28676017-28676036 None:intergenic 25.0%
! GTGAACTTTCACTTATAATT+TGG - chr1_3:28676077-28676096 Msa0113010:intron 25.0%
! GGAGTAATATGAATTAGAAA+TGG + chr1_3:28676126-28676145 None:intergenic 25.0%
!! TTAACCTTTCTACTCTATTT+TGG - chr1_3:28676208-28676227 Msa0113010:intron 25.0%
! TAAACCAAAATAGAGTAGAA+AGG + chr1_3:28676215-28676234 None:intergenic 25.0%
!! GTCAATTTTTCTGCATTTTT+TGG + chr1_3:28676507-28676526 None:intergenic 25.0%
!!! ATTTGTTTTATGTATGTCCT+CGG - chr1_3:28676585-28676604 Msa0113010:intron 25.0%
! TGTCAAATAAATAGGAGATT+TGG - chr1_3:28676617-28676636 Msa0113010:intron 25.0%
! GTCAAATAAATAGGAGATTT+GGG - chr1_3:28676618-28676637 Msa0113010:intron 25.0%
!!! TTGTAGTTTTTTTAGGGAAT+TGG - chr1_3:28676764-28676783 Msa0113010:CDS 25.0%
!!! TGTAGTTTTTTTAGGGAATT+GGG - chr1_3:28676765-28676784 Msa0113010:CDS 25.0%
! AATGCTTTAACAATATGTCA+TGG - chr1_3:28676847-28676866 Msa0113010:CDS 25.0%
! ACAATGAAATGCACATTTAT+AGG - chr1_3:28676884-28676903 Msa0113010:intron 25.0%
! TTTATCAAATACATATGCCA+TGG + chr1_3:28676959-28676978 None:intergenic 25.0%
! ACATTAACTATTCTCAGTAA+TGG + chr1_3:28677009-28677028 None:intergenic 25.0%
! ATAGTTAATGTTTAGCTGAA+TGG - chr1_3:28677018-28677037 Msa0113010:intron 25.0%
! TAACATGAGGGTTAATATAA+AGG - chr1_3:28677087-28677106 Msa0113010:intron 25.0%
!!! GCTATTTTAAAACAACTTCA+TGG - chr1_3:28677448-28677467 Msa0113010:intron 25.0%
!! TTTACTGTCGCAAATTTTAA+AGG + chr1_3:28677551-28677570 None:intergenic 25.0%
! AATGAAAACGTATCTATTAC+CGG + chr1_3:28677830-28677849 None:intergenic 25.0%
! ACATACACAAAGTTATAGTT+AGG + chr1_3:28677871-28677890 None:intergenic 25.0%
!! GAGTTGTCAATAAAATTTTG+AGG + chr1_3:28678095-28678114 None:intergenic 25.0%
!!! TATTGACAACTCTTTTAAGA+AGG - chr1_3:28678103-28678122 Msa0113010:intron 25.0%
!!! ATTGACAACTCTTTTAAGAA+GGG - chr1_3:28678104-28678123 Msa0113010:intron 25.0%
!!! TTAATTTCTTTTGGGAATTG+AGG - chr1_3:28678221-28678240 Msa0113010:intron 25.0%
!! GTATTGATAGATCTCAATTA+TGG - chr1_3:28678390-28678409 Msa0113010:intron 25.0%
!!! TTTTGAAAGTGGTTCAAAAT+AGG + chr1_3:28678526-28678545 None:intergenic 25.0%
!!! CCTAAACAAAATTTTGAAAG+TGG + chr1_3:28678537-28678556 None:intergenic 25.0%
!!! CCACTTTCAAAATTTTGTTT+AGG - chr1_3:28678534-28678553 Msa0113010:intron 25.0%
! AAAAAATCCTTATCATGTGA+AGG + chr1_3:28678583-28678602 None:intergenic 25.0%
!!! TTCACATGATAAGGATTTTT+TGG - chr1_3:28678582-28678601 Msa0113010:intron 25.0%
! AATTTACTGTTGAAATGCAA+AGG - chr1_3:28678773-28678792 Msa0113010:intron 25.0%
! GCTTAAAAAGAAAAAGGAAA+GGG + chr1_3:28679030-28679049 None:intergenic 25.0%
! AAAGAAATGATAACATGCAA+TGG + chr1_3:28679052-28679071 None:intergenic 25.0%
! ATGTTATCATTTCTTTGCTT+TGG - chr1_3:28679056-28679075 Msa0113010:intron 25.0%
! GGAGAAAAAAAATTGTAATG+AGG + chr1_3:28679422-28679441 None:intergenic 25.0%
!!! ATTGGTTTTTATGTTTGAGT+AGG - chr1_3:28679561-28679580 Msa0113010:five_prime_UTR 25.0%
! AGGAGTAGAAAAATATGTAT+TGG - chr1_3:28679657-28679676 Msa0113010:five_prime_UTR 25.0%
! TCCTAGAGAATTATGAAAAA+AGG + chr1_3:28679735-28679754 None:intergenic 25.0%
AACAAAATTCAGAGAAAGAG+AGG + chr1_3:28675562-28675581 None:intergenic 30.0%
AATGAAGGAAGAAGATAGAT+AGG + chr1_3:28675585-28675604 None:intergenic 30.0%
TAGAGATTAGTAGAAGATAG+GGG + chr1_3:28675633-28675652 None:intergenic 30.0%
GTAGAGATTAGTAGAAGATA+GGG + chr1_3:28675634-28675653 None:intergenic 30.0%
TGTAGAGATTAGTAGAAGAT+AGG + chr1_3:28675635-28675654 None:intergenic 30.0%
ACAATATATCAAACAGCTTG+TGG + chr1_3:28676053-28676072 None:intergenic 30.0%
AAATCAAGCACATAACTCAA+TGG + chr1_3:28676290-28676309 None:intergenic 30.0%
GACTTCTCTGTCAAATAAAT+AGG - chr1_3:28676609-28676628 Msa0113010:intron 30.0%
GGAGATTTGGGTATTATAAA+AGG - chr1_3:28676630-28676649 Msa0113010:intron 30.0%
! CATCTACATGGATTGATTAT+TGG - chr1_3:28676677-28676696 Msa0113010:intron 30.0%
! TATTTTATTGTTCTGCTTGC+AGG - chr1_3:28676808-28676827 Msa0113010:CDS 30.0%
!! AATTTCAAGTTTGCAATGTC+AGG - chr1_3:28676977-28676996 Msa0113010:intron 30.0%
ATGATTTATGGGTAACATGA+AGG - chr1_3:28677061-28677080 Msa0113010:intron 30.0%
!! CATTTTTCAAACATCTGGAA+TGG + chr1_3:28677256-28677275 None:intergenic 30.0%
CATTCCAGATGTTTGAAAAA+TGG - chr1_3:28677254-28677273 Msa0113010:intron 30.0%
!! TGAACCATTTTTCAAACATC+TGG + chr1_3:28677261-28677280 None:intergenic 30.0%
! TGGAAATAAGATTTGCTTAG+TGG + chr1_3:28677327-28677346 None:intergenic 30.0%
!!! ATAGAGTTTGGCTTTTTGAT+TGG - chr1_3:28677426-28677445 Msa0113010:intron 30.0%
TTGATCCCATCAAAAAAAGT+CGG + chr1_3:28677619-28677638 None:intergenic 30.0%
! TTGATGGGATCAAAACTTTT+AGG - chr1_3:28677626-28677645 Msa0113010:intron 30.0%
AAGAGATTTATGGTCATTCT+TGG + chr1_3:28677771-28677790 None:intergenic 30.0%
ATGAAAACGTATCTATTACC+GGG + chr1_3:28677829-28677848 None:intergenic 30.0%
!! TTGACAACTCTTTTAAGAAG+GGG - chr1_3:28678105-28678124 Msa0113010:intron 30.0%
AATTCTTGAAAGCAGAATCT+CGG - chr1_3:28678350-28678369 Msa0113010:intron 30.0%
TCAATTTCAAGCTACTTCTA+AGG - chr1_3:28678660-28678679 Msa0113010:intron 30.0%
TAAAATAGAGATGAGTACCT+TGG + chr1_3:28678703-28678722 None:intergenic 30.0%
! GTACTCATCTCTATTTTATG+AGG - chr1_3:28678705-28678724 Msa0113010:intron 30.0%
! TTTTATGAGGTCATTCTTTG+AGG - chr1_3:28678718-28678737 Msa0113010:intron 30.0%
AACTTCTATGCACAACAATT+GGG + chr1_3:28678752-28678771 None:intergenic 30.0%
AAACTTCTATGCACAACAAT+TGG + chr1_3:28678753-28678772 None:intergenic 30.0%
GGCTTAAAAAGAAAAAGGAA+AGG + chr1_3:28679031-28679050 None:intergenic 30.0%
GCAATGGCTTAAAAAGAAAA+AGG + chr1_3:28679036-28679055 None:intergenic 30.0%
TATCATTTCTTTGCTTTGGT+TGG - chr1_3:28679060-28679079 Msa0113010:intron 30.0%
TTGAAGTGGTTAAATAGAGA+AGG + chr1_3:28679131-28679150 None:intergenic 30.0%
ACAGTGAGAACAAAAATAAG+TGG + chr1_3:28679175-28679194 None:intergenic 30.0%
! ACTCTTCTTTAATTCCCTTT+TGG - chr1_3:28679217-28679236 Msa0113010:intron 30.0%
ATCCTATGCAAAAACAATGA+GGG + chr1_3:28679252-28679271 None:intergenic 30.0%
TATCCTATGCAAAAACAATG+AGG + chr1_3:28679253-28679272 None:intergenic 30.0%
TCAATCTTCAACATCATAGT+TGG + chr1_3:28679373-28679392 None:intergenic 30.0%
!!! CAATTTTTTTTCTCCTTTGC+AGG - chr1_3:28679427-28679446 Msa0113010:CDS 30.0%
AATGAAAATAGTTCCTGCAA+AGG + chr1_3:28679443-28679462 None:intergenic 30.0%
! TTGCAGGAACTATTTTCATT+TGG - chr1_3:28679443-28679462 Msa0113010:CDS 30.0%
!! GTCATTTGAGCTTTGAAATA+AGG - chr1_3:28679599-28679618 Msa0113010:five_prime_UTR 30.0%
!!! GCCTTTTTTCATAATTCTCT+AGG - chr1_3:28679731-28679750 Msa0113010:five_prime_UTR 30.0%
TTTGGATATTGTCTATTGCA+TGG - chr1_3:28679841-28679860 Msa0113010:five_prime_UTR 30.0%
! GAAACATAAGAGTATGAGCA+TGG + chr1_3:28675725-28675744 None:intergenic 35.0%
GGAATCTAATACGATACACA+AGG - chr1_3:28675795-28675814 Msa0113010:three_prime_UTR 35.0%
TTACTCTTCTCCAATGAATG+AGG - chr1_3:28675820-28675839 Msa0113010:three_prime_UTR 35.0%
TCTGTAATCACCTCATTCAT+TGG + chr1_3:28675833-28675852 None:intergenic 35.0%
TTCTCATGAGAACAAGCAAA+AGG - chr1_3:28675979-28675998 Msa0113010:intron 35.0%
TGAACTCCATATAACAACGT+TGG - chr1_3:28676173-28676192 Msa0113010:intron 35.0%
GTGTAACCAACGTTGTTATA+TGG + chr1_3:28676182-28676201 None:intergenic 35.0%
! AACAACGTTGGTTACACAAT+TGG - chr1_3:28676185-28676204 Msa0113010:intron 35.0%
AATATCTGTGTGGATAGGTT+AGG - chr1_3:28676263-28676282 Msa0113010:intron 35.0%
CAATATGTCATGGCTTCATT+TGG - chr1_3:28676857-28676876 Msa0113010:intron 35.0%
AACATGAAGGAAGTAACATG+AGG - chr1_3:28677074-28677093 Msa0113010:intron 35.0%
ACATGAAGGAAGTAACATGA+GGG - chr1_3:28677075-28677094 Msa0113010:intron 35.0%
! ATGTTGTAGAGGATGGTTTT+TGG + chr1_3:28677117-28677136 None:intergenic 35.0%
ATTCTATCTCTGATCTGATG+TGG - chr1_3:28677208-28677227 Msa0113010:intron 35.0%
! CATGGTAACTCTCTCTTTTT+AGG - chr1_3:28677466-28677485 Msa0113010:intron 35.0%
!!! AAGCACCGACTTTTTTTGAT+GGG - chr1_3:28677611-28677630 Msa0113010:intron 35.0%
AACTTTGTGTATGTCTCATC+AGG - chr1_3:28677877-28677896 Msa0113010:intron 35.0%
!!! AAAAGTTTTAGCTATGGCAG+TGG + chr1_3:28677915-28677934 None:intergenic 35.0%
! TAAAGTGCAACTTGCTTGAA+AGG - chr1_3:28677954-28677973 Msa0113010:intron 35.0%
! CTCTAAAGAGATGTCAATCA+GGG + chr1_3:28678136-28678155 None:intergenic 35.0%
CTGATTGACATCTCTTTAGA+GGG - chr1_3:28678135-28678154 Msa0113010:intron 35.0%
!! ATTTCTTTTGGGAATTGAGG+TGG - chr1_3:28678224-28678243 Msa0113010:intron 35.0%
GATTCTGCTTTCAAGAATTC+TGG + chr1_3:28678349-28678368 None:intergenic 35.0%
!! AGTTTGCTTTTGTAAGTGTC+TGG - chr1_3:28678453-28678472 Msa0113010:intron 35.0%
TCAACTACCTTCACATGATA+AGG - chr1_3:28678573-28678592 Msa0113010:intron 35.0%
TACCTTGGACTTGAAAATGA+TGG + chr1_3:28678688-28678707 None:intergenic 35.0%
GTGAATGATGAAATTTCGTG+AGG - chr1_3:28678904-28678923 Msa0113010:intron 35.0%
AGAAAAAGGAAAGGGATACA+GGG + chr1_3:28679022-28679041 None:intergenic 35.0%
AAGAAAAAGGAAAGGGATAC+AGG + chr1_3:28679023-28679042 None:intergenic 35.0%
!!! ATCCCTCATTGTTTTTGCAT+AGG - chr1_3:28679247-28679266 Msa0113010:intron 35.0%
!! AATATGGATATGATGGACAG+TGG - chr1_3:28679290-28679309 Msa0113010:intron 35.0%
CACACTTGCAAAAAATGAGA+TGG - chr1_3:28679314-28679333 Msa0113010:intron 35.0%
! TGTATTCTGCACTTTGCTAT+TGG - chr1_3:28679475-28679494 Msa0113010:exon 35.0%
ATGTTTGAGTAGGAAGAATG+AGG - chr1_3:28679571-28679590 Msa0113010:five_prime_UTR 35.0%
! TGATGATGTTCTCTTAAGCA+TGG - chr1_3:28679630-28679649 Msa0113010:five_prime_UTR 35.0%
GTTCTCTTAAGCATGGAATT+AGG - chr1_3:28679637-28679656 Msa0113010:five_prime_UTR 35.0%
TATGCGATGCACAATTATAG+AGG - chr1_3:28679762-28679781 Msa0113010:five_prime_UTR 35.0%
ATGCGATGCACAATTATAGA+GGG - chr1_3:28679763-28679782 Msa0113010:five_prime_UTR 35.0%
TGTCTATTGCATGGTCTTTA+CGG - chr1_3:28679850-28679869 Msa0113010:five_prime_UTR 35.0%
AGAGAAAGAGAGGAAGATAG+AGG + chr1_3:28675552-28675571 None:intergenic 40.0%
AGTAGAAGATAGGGGAAAAG+AGG + chr1_3:28675625-28675644 None:intergenic 40.0%
! GCATTAACTTTCTGGAGGTA+AGG + chr1_3:28675703-28675722 None:intergenic 40.0%
TGAGCATGGCATTAACTTTC+TGG + chr1_3:28675711-28675730 None:intergenic 40.0%
AACGCTAGTGGAGTTGAAAA+GGG + chr1_3:28675750-28675769 None:intergenic 40.0%
TAGTAGAGGCAATATCTGTG+TGG - chr1_3:28676253-28676272 Msa0113010:intron 40.0%
ACTTGATACATCTTGTGCAC+TGG + chr1_3:28676396-28676415 None:intergenic 40.0%
! AGTCAAGAAGCACATTTGCT+TGG - chr1_3:28676420-28676439 Msa0113010:intron 40.0%
TTGGGTATTCTTTCTAGACC+TGG + chr1_3:28676488-28676507 None:intergenic 40.0%
! AACCGAGTACATTGACTAAC+TGG - chr1_3:28676550-28676569 Msa0113010:intron 40.0%
CACCAGTTAGTCAATGTACT+CGG + chr1_3:28676555-28676574 None:intergenic 40.0%
! TGGACTTGATGACATCTACA+TGG - chr1_3:28676665-28676684 Msa0113010:intron 40.0%
AGTGCAAATGTTGTAGAGGA+TGG + chr1_3:28677124-28677143 None:intergenic 40.0%
! AGGAAGTGCAAATGTTGTAG+AGG + chr1_3:28677128-28677147 None:intergenic 40.0%
!!! GAAGCACCGACTTTTTTTGA+TGG - chr1_3:28677610-28677629 Msa0113010:intron 40.0%
TGAAGCAGAAACATGAAGAG+TGG - chr1_3:28677699-28677718 Msa0113010:intron 40.0%
! CCTCTAAAGAGATGTCAATC+AGG + chr1_3:28678137-28678156 None:intergenic 40.0%
CCTGATTGACATCTCTTTAG+AGG - chr1_3:28678134-28678153 Msa0113010:intron 40.0%
GCTGCTCAATCATAATGAAG+TGG - chr1_3:28678246-28678265 Msa0113010:intron 40.0%
! TCATAATGAAGTGGAAGCGT+AGG - chr1_3:28678255-28678274 Msa0113010:intron 40.0%
TTCTTGAACGTGGAATCAGA+GGG - chr1_3:28678302-28678321 Msa0113010:intron 40.0%
AAGCAGAATCTCGGCTTATT+AGG - chr1_3:28678359-28678378 Msa0113010:intron 40.0%
GTCTACAACTTTGACATGAC+AGG + chr1_3:28678636-28678655 None:intergenic 40.0%
!!! CACCATCATTTTCAAGTCCA+AGG - chr1_3:28678683-28678702 Msa0113010:intron 40.0%
!! GCTTTGGTTGGTTCTATACA+TGG - chr1_3:28679072-28679091 Msa0113010:intron 40.0%
TGCAAAAACAATGAGGGATG+AGG + chr1_3:28679246-28679265 None:intergenic 40.0%
AAAAGAGGATCAGAAGGCAG+GGG + chr1_3:28675610-28675629 None:intergenic 45.0%
GAAAAGAGGATCAGAAGGCA+GGG + chr1_3:28675611-28675630 None:intergenic 45.0%
TAGGGGAAAAGAGGATCAGA+AGG + chr1_3:28675616-28675635 None:intergenic 45.0%
AAGGACAACCGAAGGAGTAA+GGG + chr1_3:28675684-28675703 None:intergenic 45.0%
TAAGGACAACCGAAGGAGTA+AGG + chr1_3:28675685-28675704 None:intergenic 45.0%
GCATGGCATTAACTTTCTGG+AGG + chr1_3:28675708-28675727 None:intergenic 45.0%
ACGCTAGTGGAGTTGAAAAG+GGG + chr1_3:28675749-28675768 None:intergenic 45.0%
CAACGCTAGTGGAGTTGAAA+AGG + chr1_3:28675751-28675770 None:intergenic 45.0%
GAGGCAATATCTGTGTGGAT+AGG - chr1_3:28676258-28676277 Msa0113010:intron 45.0%
GCCACTACACCAATTTCACA+CGG - chr1_3:28676462-28676481 Msa0113010:intron 45.0%
ATTTGACAGAGAAGTCTCCG+AGG + chr1_3:28676605-28676624 None:intergenic 45.0%
TGCTTGCAGGTAGTGTTAAG+TGG - chr1_3:28676821-28676840 Msa0113010:CDS 45.0%
! ATACAAAAAGCGTGAGCACC+CGG - chr1_3:28677808-28677827 Msa0113010:intron 45.0%
!! TAGCTATGGCAGTGGTTTTG+TGG + chr1_3:28677907-28677926 None:intergenic 45.0%
CTTCTTGAACGTGGAATCAG+AGG - chr1_3:28678301-28678320 Msa0113010:intron 45.0%
!! AGAAGGTGCTAATGAGTGAG+AGG + chr1_3:28679114-28679133 None:intergenic 45.0%
!!! ATAACAAATTTAATTTAATA+TGG - chr1_3:28679274-28679293 Msa0113010:intron 5.0%
CAGAAGGCAGGGGATAATGA+AGG + chr1_3:28675600-28675619 None:intergenic 50.0%
GGAAAAGAGGATCAGAAGGC+AGG + chr1_3:28675612-28675631 None:intergenic 50.0%
AGGACAACCGAAGGAGTAAG+GGG + chr1_3:28675683-28675702 None:intergenic 50.0%
! TCCACTAGCGTTGAACTCGA+TGG - chr1_3:28675758-28675777 Msa0113010:three_prime_UTR 50.0%
ACCATCGAGTTCAACGCTAG+TGG + chr1_3:28675762-28675781 None:intergenic 50.0%
! CTAGCGTTGAACTCGATGGT+CGG - chr1_3:28675762-28675781 Msa0113010:three_prime_UTR 50.0%
GCCGTGTGAAATTGGTGTAG+TGG + chr1_3:28676466-28676485 None:intergenic 50.0%
TACACCAATTTCACACGGCC+AGG - chr1_3:28676467-28676486 Msa0113010:intron 50.0%
TAGACCTGGCCGTGTGAAAT+TGG + chr1_3:28676474-28676493 None:intergenic 50.0%
AAGCGTAGGAGATCGAGTGA+TGG - chr1_3:28678269-28678288 Msa0113010:intron 50.0%
GTAGGAGATCGAGTGATGGT+TGG - chr1_3:28678273-28678292 Msa0113010:intron 50.0%
TTGGCAGTGCTTCTTGAACG+TGG - chr1_3:28678292-28678311 Msa0113010:intron 50.0%
ATCAGAGGGAGCAAAGACTG+TGG - chr1_3:28678316-28678335 Msa0113010:intron 50.0%
TCAGAGGGAGCAAAGACTGT+GGG - chr1_3:28678317-28678336 Msa0113010:intron 50.0%
GAGGGATGAGGAAGCCAAAA+GGG + chr1_3:28679234-28679253 None:intergenic 50.0%
TGAGGGATGAGGAAGCCAAA+AGG + chr1_3:28679235-28679254 None:intergenic 50.0%
! GTGGCAGTTTTGCAGTGAGG+AGG + chr1_3:28675661-28675680 None:intergenic 55.0%
ACTGCCACCCCTTACTCCTT+CGG - chr1_3:28675673-28675692 Msa0113010:three_prime_UTR 55.0%
ACAACCGAAGGAGTAAGGGG+TGG + chr1_3:28675680-28675699 None:intergenic 55.0%
CTGGAGGTAAGGACAACCGA+AGG + chr1_3:28675692-28675711 None:intergenic 55.0%
TCGATGGTCGGTCATAGCGA+GGG - chr1_3:28675774-28675793 Msa0113010:three_prime_UTR 55.0%
CAGAGGGAGCAAAGACTGTG+GGG - chr1_3:28678318-28678337 Msa0113010:intron 55.0%
AGAGGGAGCAAAGACTGTGG+GGG - chr1_3:28678319-28678338 Msa0113010:intron 55.0%
! GGGGTGGCAGTTTTGCAGTG+AGG + chr1_3:28675664-28675683 None:intergenic 60.0%
CTCGATGGTCGGTCATAGCG+AGG - chr1_3:28675773-28675792 Msa0113010:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 28675549 28679875 28675549 ID=Msa0113010;Name=Msa0113010
chr1_3 mRNA 28675549 28679875 28675549 ID=Msa0113010-mRNA-1;Parent=Msa0113010;Name=Msa0113010-mRNA-1;_AED=0.31;_eAED=0.31;_QI=386|0.75|1|1|0|0|5|376|82
chr1_3 exon 28675549 28675841 28675549 ID=Msa0113010-mRNA-1:exon:9456;Parent=Msa0113010-mRNA-1
chr1_3 exon 28675926 28675975 28675926 ID=Msa0113010-mRNA-1:exon:9455;Parent=Msa0113010-mRNA-1
chr1_3 exon 28676089 28676155 28676089 ID=Msa0113010-mRNA-1:exon:9454;Parent=Msa0113010-mRNA-1
chr1_3 exon 28676720 28676868 28676720 ID=Msa0113010-mRNA-1:exon:9453;Parent=Msa0113010-mRNA-1
chr1_3 exon 28679424 28679875 28679424 ID=Msa0113010-mRNA-1:exon:9452;Parent=Msa0113010-mRNA-1
chr1_3 five_prime_UTR 28679490 28679875 28679490 ID=Msa0113010-mRNA-1:five_prime_utr;Parent=Msa0113010-mRNA-1
chr1_3 CDS 28679424 28679489 28679424 ID=Msa0113010-mRNA-1:cds;Parent=Msa0113010-mRNA-1
chr1_3 CDS 28676720 28676868 28676720 ID=Msa0113010-mRNA-1:cds;Parent=Msa0113010-mRNA-1
chr1_3 CDS 28676122 28676155 28676122 ID=Msa0113010-mRNA-1:cds;Parent=Msa0113010-mRNA-1
chr1_3 three_prime_UTR 28676089 28676121 28676089 ID=Msa0113010-mRNA-1:three_prime_utr;Parent=Msa0113010-mRNA-1
chr1_3 three_prime_UTR 28675926 28675975 28675926 ID=Msa0113010-mRNA-1:three_prime_utr;Parent=Msa0113010-mRNA-1
chr1_3 three_prime_UTR 28675549 28675841 28675549 ID=Msa0113010-mRNA-1:three_prime_utr;Parent=Msa0113010-mRNA-1
Gene Sequence

>Msa0113010

ATGTTCACGTTCCATGTTTTTTTTTTTGTTTCAACAATGGAAAATTCTCATGAGAACAAGCAAAAGGTGAGTGTATATTTTGATCAACTACCTTCACATGATAAGGATTTTTTGGACTTAATTCATAGTAAGACTATTGGATTCCTGTCATGTCAAAGTTGTAGACTGCTTCAATTTCAAGCTACTTCTAAGGCACCATCATTTTCAAGTCCAAGGATATATAACAAATTTAATTTAATATGGATATGA
Protein sequence

>Msa0113010

MFTFHVFFFVSTMENSHENKQKVSVYFDQLPSHDKDFLDLIHSKTIGFLSCQSCRLLQFQATSKAPSFSSPRIYNKFNLIWI*