AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0289510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 56 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCATTTGAGCTTTGAAATA+AGG 0.154303 2_3:-35759088 Msa0289510:three_prime_UTR
ATAACAAATTTAATTTAATA+TGG 0.185007 2_3:-35759413 Msa0289510:CDS
GGAGCATGGCATTAACTTTC+TGG 0.185088 2_3:+35762785 None:intergenic
TGTAGAGATTAGTAGAAGAT+AGG 0.251868 2_3:+35762861 None:intergenic
GTTCTCTTAAGCATGGAATT+AGG 0.287198 2_3:-35759050 Msa0289510:three_prime_UTR
TCAATTTCAAGCTACTTCTA+AGG 0.290144 2_3:-35760028 Msa0289510:CDS
TGTCTATTGCATGGTCTTTA+CGG 0.300319 2_3:-35758836 Msa0289510:three_prime_UTR
TGTATTCTGCACTTTGCTAT+TGG 0.313600 2_3:-35759212 Msa0289510:three_prime_UTR
TTAATTCATAGTAAGACTAT+TGG 0.329902 2_3:-35760081 Msa0289510:CDS
TTAATTTAATATGGATATGA+TGG 0.345164 2_3:-35759404 Msa0289510:exon
AATGAAGGAAGAAGATAGAT+AGG 0.355035 2_3:+35762911 None:intergenic
GTAGAGATTAGTAGAAGATA+GGG 0.389518 2_3:+35762862 None:intergenic
TCAACTACCTTCACATGATA+AGG 0.413493 2_3:-35760115 Msa0289510:CDS
CAACGCTAGTGGAGTTGAAA+AGG 0.435330 2_3:+35762734 None:intergenic
TGAGGACAACCGAAGGAGTA+AGG 0.449016 2_3:+35762811 None:intergenic
TTTGGATATTGTCTATTGCA+TGG 0.458642 2_3:-35758845 Msa0289510:three_prime_UTR
TCTGTAATCACCTCATTCAT+TGG 0.462131 2_3:+35762654 None:intergenic
TTAGATTCCCTCGCTATGTC+CGG 0.474234 2_3:+35762701 None:intergenic
ACTGCCACCCCTTACTCCTT+CGG 0.475857 2_3:-35762820 Msa0289510:five_prime_UTR
AGAGAAAGAGAGGAAGATAG+AGG 0.488441 2_3:+35762944 None:intergenic
AACGCTAGTGGAGTTGAAAA+GGG 0.493587 2_3:+35762735 None:intergenic
CATAAGAGTATGAGCATGGC+AGG 0.493624 2_3:+35762764 None:intergenic
GGAAAAGAAGATCAGAAGGC+AGG 0.502211 2_3:+35762884 None:intergenic
AACAAAATTCAGAGAAAGAG+AGG 0.514132 2_3:+35762934 None:intergenic
ATGTTTGAGTAGGAAGAATG+AGG 0.515310 2_3:-35759116 Msa0289510:intron
GAAAGAGAGGAAGATAGAGG+AGG 0.519024 2_3:+35762947 None:intergenic
TTACTCTTTACCAATGAATG+AGG 0.521806 2_3:-35762664 Msa0289510:five_prime_UTR
TTCTCATGAGAACAAGCAAA+AGG 0.529960 2_3:-35762503 Msa0289510:intron
GTCTACAACTTTGACATGAC+AGG 0.532540 2_3:+35760055 None:intergenic
TGATGATGTTCTCTTAAGCA+TGG 0.545293 2_3:-35759057 Msa0289510:three_prime_UTR
CTCGATGGCCGGACATAGCG+AGG 0.550349 2_3:-35762709 Msa0289510:five_prime_UTR
GAAAAGAAGATCAGAAGGCA+GGG 0.555147 2_3:+35762885 None:intergenic
GAGGACAACCGAAGGAGTAA+GGG 0.557009 2_3:+35762812 None:intergenic
CAAAATGTAAAAGAATCTAA+AGG 0.559695 2_3:+35762630 None:intergenic
ATGTGATGCACAATTATAGA+GGG 0.566077 2_3:-35758923 Msa0289510:three_prime_UTR
CAGAAGGCAGGGGATAATGA+AGG 0.569984 2_3:+35762896 None:intergenic
GCCATCGAGTTCAACGCTAG+TGG 0.578022 2_3:+35762723 None:intergenic
TAGAGATTAGTAGAAGATAG+GGG 0.580074 2_3:+35762863 None:intergenic
TAGGGGAAAAGAAGATCAGA+AGG 0.580111 2_3:+35762880 None:intergenic
CTAGCGTTGAACTCGATGGC+CGG 0.583376 2_3:-35762720 Msa0289510:five_prime_UTR
TATGTGATGCACAATTATAG+AGG 0.584196 2_3:-35758924 Msa0289510:three_prime_UTR
GCATTAACTTTCTGGAGGTG+AGG 0.592971 2_3:+35762793 None:intergenic
CTATTGCATGGTCTTTACGG+TGG 0.599039 2_3:-35758833 Msa0289510:three_prime_UTR
GTATGAGCATGGCAGGAGCA+TGG 0.612216 2_3:+35762771 None:intergenic
TCCACTAGCGTTGAACTCGA+TGG 0.615861 2_3:-35762724 Msa0289510:five_prime_UTR
TACCTTGGACTTGAAAATGA+TGG 0.619298 2_3:+35760003 None:intergenic
GAAACATAAGAGTATGAGCA+TGG 0.621463 2_3:+35762760 None:intergenic
AGGACAACCGAAGGAGTAAG+GGG 0.630369 2_3:+35762813 None:intergenic
ACGCTAGTGGAGTTGAAAAG+GGG 0.650616 2_3:+35762736 None:intergenic
GCATGGCATTAACTTTCTGG+AGG 0.652172 2_3:+35762788 None:intergenic
AAAAGAAGATCAGAAGGCAG+GGG 0.661495 2_3:+35762886 None:intergenic
CTGGAGGTGAGGACAACCGA+AGG 0.667343 2_3:+35762804 None:intergenic
TCGATGGCCGGACATAGCGA+GGG 0.689004 2_3:-35762708 Msa0289510:five_prime_UTR
AATATGGATATGATGGACAG+TGG 0.692254 2_3:-35759397 Msa0289510:exon
ACAACCGAAGGAGTAAGGGG+TGG 0.701061 2_3:+35762816 None:intergenic
GGAATCTAATACGATACACA+AGG 0.732293 2_3:-35762687 Msa0289510:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAACTAAAAATGATATA+TGG + chr2_3:35759442-35759461 None:intergenic 10.0%
!!! TATATGATTTTAATTTCTTT+TGG - chr2_3:35761298-35761317 Msa0289510:intron 10.0%
!!! ATATGATTTTAATTTCTTTT+GGG - chr2_3:35761299-35761318 Msa0289510:intron 10.0%
!!! AAATTTTCTTAAACTTAAAA+GGG + chr2_3:35762774-35762793 None:intergenic 10.0%
!!! AAAATTTTCTTAAACTTAAA+AGG + chr2_3:35762775-35762794 None:intergenic 10.0%
!! GAAACAAAAAAAAAAAAACA+TGG + chr2_3:35759242-35759261 None:intergenic 15.0%
!!! TTTTTTTTTTGTTTCAACAA+TGG - chr2_3:35759247-35759266 Msa0289510:intron 15.0%
!!! TTTTATTGATTAAATAGTAG+AGG - chr2_3:35759534-35759553 Msa0289510:intron 15.0%
!!! ATTGTTGTAGTTTTTTTTTA+TGG - chr2_3:35759842-35759861 Msa0289510:intron 15.0%
!!! TAGTTTTTTTTTATGGAATT+GGG - chr2_3:35759849-35759868 Msa0289510:intron 15.0%
!!! ATACATTTTAAGATGATTTA+TGG - chr2_3:35760133-35760152 Msa0289510:CDS 15.0%
!!! TACATTTTAAGATGATTTAT+GGG - chr2_3:35760134-35760153 Msa0289510:CDS 15.0%
!! TAAAATAAAAAATGCACAAA+AGG + chr2_3:35760232-35760251 None:intergenic 15.0%
!!! TTCTTATAACATTATTTTCA+TGG + chr2_3:35760431-35760450 None:intergenic 15.0%
!!! ATGCTGATTTAATTAAATTT+TGG - chr2_3:35760818-35760837 Msa0289510:intron 15.0%
!!! TGCTGATTTAATTAAATTTT+GGG - chr2_3:35760819-35760838 Msa0289510:intron 15.0%
!! GAGATAATATCAAAAAATTA+AGG + chr2_3:35762065-35762084 None:intergenic 15.0%
!! TTACAAAATTTAACTAACTA+AGG + chr2_3:35762087-35762106 None:intergenic 15.0%
!! TTAATTTAATATGGATATGA+TGG - chr2_3:35762373-35762392 Msa0289510:intron 15.0%
!!! AAACAATGTTGTTAATTTTT+TGG - chr2_3:35762486-35762505 Msa0289510:intron 15.0%
!!! TGATATTTGATGTATTTTTT+TGG - chr2_3:35762914-35762933 Msa0289510:five_prime_UTR 15.0%
!! CAAAATGTAAAAGAATCTAA+AGG + chr2_3:35759150-35759169 None:intergenic 20.0%
!!! CTTTAGATTCTTTTACATTT+TGG - chr2_3:35759148-35759167 Msa0289510:intron 20.0%
!! GAGTATTATGAATTAGAAAT+GGG + chr2_3:35759420-35759439 None:intergenic 20.0%
!! ATGAAATTACAATTGTTCAA+AGG + chr2_3:35759628-35759647 None:intergenic 20.0%
!!! GTAGTTTTTTTTTATGGAAT+TGG - chr2_3:35759848-35759867 Msa0289510:intron 20.0%
!! TTTATCAAATACATATGTCA+TGG + chr2_3:35760043-35760062 None:intergenic 20.0%
!!! TTTGATTTCTCATTTTACTA+TGG - chr2_3:35760257-35760276 Msa0289510:intron 20.0%
!!! TACTTTTACTAAATAGAGTT+TGG - chr2_3:35760498-35760517 Msa0289510:intron 20.0%
!! TCAACTTCTAGATAAAAAAA+AGG + chr2_3:35760606-35760625 None:intergenic 20.0%
!! AAACTTTATCAAGAGATTTA+TGG + chr2_3:35760867-35760886 None:intergenic 20.0%
!!! AGTTGTCAATAAAATTTTGA+GGG + chr2_3:35761180-35761199 None:intergenic 20.0%
!! TTAATTCATAGTAAGACTAT+TGG - chr2_3:35761696-35761715 Msa0289510:intron 20.0%
!! TAGTTAGATTGTGTTATATA+TGG - chr2_3:35761947-35761966 Msa0289510:intron 20.0%
!! CTTAAAAAGAAAAAGGAAAA+GGG + chr2_3:35762119-35762138 None:intergenic 20.0%
!! AGATGAATAAATTATTGAAG+TGG + chr2_3:35762235-35762254 None:intergenic 20.0%
!!! TTCCATTTTCTAATTTGTAT+TGG - chr2_3:35762633-35762652 Msa0289510:five_prime_UTR 20.0%
!! AACCAATACAAATTAGAAAA+TGG + chr2_3:35762638-35762657 None:intergenic 20.0%
!!! TTACATTTTGGAAAGCATTT+TGG - chr2_3:35759160-35759179 Msa0289510:intron 25.0%
! GAAAAAACTTATTCACAAAG+AGG + chr2_3:35759312-35759331 None:intergenic 25.0%
! GTGAACTTTCACTTATAATT+TGG - chr2_3:35759372-35759391 Msa0289510:intron 25.0%
!! GGAGTATTATGAATTAGAAA+TGG + chr2_3:35759421-35759440 None:intergenic 25.0%
!! TTAACCTTTCTACTCTATTT+TGG - chr2_3:35759503-35759522 Msa0289510:intron 25.0%
! TAAACCAAAATAGAGTAGAA+AGG + chr2_3:35759510-35759529 None:intergenic 25.0%
! CATTTGCTTATTGGTAAATA+TGG - chr2_3:35759727-35759746 Msa0289510:intron 25.0%
! TATGCTTTAACAATATGTCA+TGG - chr2_3:35759931-35759950 Msa0289510:intron 25.0%
! ACAATGAAATGCACATTTAT+AGG - chr2_3:35759968-35759987 Msa0289510:intron 25.0%
! ACATTAACTATTCTCAGTAA+TGG + chr2_3:35760093-35760112 None:intergenic 25.0%
! ATAGTTAATGTTTAGCTGAA+TGG - chr2_3:35760102-35760121 Msa0289510:CDS 25.0%
! TAACATGAGGGTTAATATAA+AGG - chr2_3:35760171-35760190 Msa0289510:intron 25.0%
!!! GCTATTTTAAAACAACTTCA+TGG - chr2_3:35760532-35760551 Msa0289510:intron 25.0%
!! TTTACTGTCGCAAATTTTAA+AGG + chr2_3:35760637-35760656 None:intergenic 25.0%
! ATGAAAACGTATCTATTACT+GGG + chr2_3:35760915-35760934 None:intergenic 25.0%
! AATGAAAACGTATCTATTAC+TGG + chr2_3:35760916-35760935 None:intergenic 25.0%
! ACATACACAAAGTTATAGTT+AGG + chr2_3:35760957-35760976 None:intergenic 25.0%
! GCGAAATGCATATAAAAAAT+AGG + chr2_3:35761131-35761150 None:intergenic 25.0%
!!! ATTTTTTATATGCATTTCGC+TGG - chr2_3:35761131-35761150 Msa0289510:intron 25.0%
!! GAGTTGTCAATAAAATTTTG+AGG + chr2_3:35761181-35761200 None:intergenic 25.0%
!!! TATTGACAACTCTTTTAAGA+AGG - chr2_3:35761189-35761208 Msa0289510:intron 25.0%
!!! ATTGACAACTCTTTTAAGAA+GGG - chr2_3:35761190-35761209 Msa0289510:intron 25.0%
!!! TTAATTTCTTTTGGGAATTG+AGG - chr2_3:35761307-35761326 Msa0289510:intron 25.0%
!! GTATTGATAGATCTCAATTA+TGG - chr2_3:35761476-35761495 Msa0289510:intron 25.0%
!!! CCTAAACAAAATTTTGAAAG+TGG + chr2_3:35761626-35761645 None:intergenic 25.0%
!!! CCACTTTCAAAATTTTGTTT+AGG - chr2_3:35761623-35761642 Msa0289510:intron 25.0%
! AAAAAATCCTTATCATGTGA+AGG + chr2_3:35761672-35761691 None:intergenic 25.0%
!!! TTCACATGATAAGGATTTTT+TGG - chr2_3:35761671-35761690 Msa0289510:intron 25.0%
! AATTTACTGTTGAAATGCAA+AGG - chr2_3:35761862-35761881 Msa0289510:intron 25.0%
! GCTTAAAAAGAAAAAGGAAA+AGG + chr2_3:35762120-35762139 None:intergenic 25.0%
! AAAGAAATGATAACATGCAA+TGG + chr2_3:35762142-35762161 None:intergenic 25.0%
! ATGTTATCATTTCTTTGCTT+TGG - chr2_3:35762146-35762165 Msa0289510:intron 25.0%
! GGAGAAAAAAAATTGTAATG+AGG + chr2_3:35762512-35762531 None:intergenic 25.0%
!!! ATTGGTTTTTATGTTTGAGT+AGG - chr2_3:35762651-35762670 Msa0289510:five_prime_UTR 25.0%
! AGGAGTAGAAAAATATGTAT+TGG - chr2_3:35762747-35762766 Msa0289510:five_prime_UTR 25.0%
! CCTAGAGAATTAATGAAAAA+AGG + chr2_3:35762825-35762844 None:intergenic 25.0%
!!! CCTTTTTTCATTAATTCTCT+AGG - chr2_3:35762822-35762841 Msa0289510:five_prime_UTR 25.0%
AACAAAATTCAGAGAAAGAG+AGG + chr2_3:35758846-35758865 None:intergenic 30.0%
AATGAAGGAAGAAGATAGAT+AGG + chr2_3:35758869-35758888 None:intergenic 30.0%
TAGAGATTAGTAGAAGATAG+GGG + chr2_3:35758917-35758936 None:intergenic 30.0%
GTAGAGATTAGTAGAAGATA+GGG + chr2_3:35758918-35758937 None:intergenic 30.0%
TGTAGAGATTAGTAGAAGAT+AGG + chr2_3:35758919-35758938 None:intergenic 30.0%
TTACTCTTTACCAATGAATG+AGG - chr2_3:35759113-35759132 Msa0289510:intron 30.0%
AAATCAAGCACATAACTCAA+TGG + chr2_3:35759585-35759604 None:intergenic 30.0%
! CATCTACATGGATTGATTAT+TGG - chr2_3:35759759-35759778 Msa0289510:intron 30.0%
!! AATTTCAAGTTTGCAATGTC+AGG - chr2_3:35760061-35760080 Msa0289510:CDS 30.0%
ATGATTTATGGGTAACATGA+AGG - chr2_3:35760145-35760164 Msa0289510:intron 30.0%
!! CATTTTTCAAACATCTGGAA+TGG + chr2_3:35760340-35760359 None:intergenic 30.0%
CATTCCAGATGTTTGAAAAA+TGG - chr2_3:35760338-35760357 Msa0289510:intron 30.0%
!! TGAACCATTTTTCAAACATC+TGG + chr2_3:35760345-35760364 None:intergenic 30.0%
! TGGAAATAAGATTTGCTTAG+TGG + chr2_3:35760411-35760430 None:intergenic 30.0%
!!! ATAGAGTTTGGCTTTTTGAT+TGG - chr2_3:35760510-35760529 Msa0289510:intron 30.0%
TTGATCCCATCAAAAAAAGT+CGG + chr2_3:35760705-35760724 None:intergenic 30.0%
! TTGATGGGATCAAAACTTTT+AGG - chr2_3:35760712-35760731 Msa0289510:intron 30.0%
AAGAGATTTATGGTCATTCT+TGG + chr2_3:35760857-35760876 None:intergenic 30.0%
! TCATTTGAAAGCTTTAGCTA+TGG + chr2_3:35761007-35761026 None:intergenic 30.0%
CTAAAGCTTTCAAATGATTG+AGG - chr2_3:35761010-35761029 Msa0289510:intron 30.0%
!! TCAATAAAATTTTGAGGGCA+AGG + chr2_3:35761175-35761194 None:intergenic 30.0%
!! TTGACAACTCTTTTAAGAAG+GGG - chr2_3:35761191-35761210 Msa0289510:intron 30.0%
AATTCTTGAAAGCAGAATCT+CGG - chr2_3:35761436-35761455 Msa0289510:intron 30.0%
!!! TTGCTTTTTTTGTAAGTGTC+TGG - chr2_3:35761542-35761561 Msa0289510:intron 30.0%
!! TTTTGAAAGTGGTTCAAAAG+AGG + chr2_3:35761615-35761634 None:intergenic 30.0%
TCAATTTCAAGCTACTTCTA+AGG - chr2_3:35761749-35761768 Msa0289510:intron 30.0%
TAAAATAGAGATGAGTACCT+TGG + chr2_3:35761792-35761811 None:intergenic 30.0%
! GTACTCATCTCTATTTTATG+AGG - chr2_3:35761794-35761813 Msa0289510:intron 30.0%
! TTTTATGAGGTCATTCTTTG+AGG - chr2_3:35761807-35761826 Msa0289510:intron 30.0%
AACTTCTATGCACAACAATT+GGG + chr2_3:35761841-35761860 None:intergenic 30.0%
AAACTTCTATGCACAACAAT+TGG + chr2_3:35761842-35761861 None:intergenic 30.0%
GCAATGGCTTAAAAAGAAAA+AGG + chr2_3:35762126-35762145 None:intergenic 30.0%
TATCATTTCTTTGCTTTGGT+TGG - chr2_3:35762150-35762169 Msa0289510:intron 30.0%
TTGAAGTGGTTAAATAGAGA+AGG + chr2_3:35762221-35762240 None:intergenic 30.0%
ACAGTGAGAACAAAAATAAG+TGG + chr2_3:35762265-35762284 None:intergenic 30.0%
! ACTCTTCTTTAATTCCCTTT+TGG - chr2_3:35762307-35762326 Msa0289510:intron 30.0%
ATCCTATGCAAAAACAATGA+GGG + chr2_3:35762342-35762361 None:intergenic 30.0%
TATCCTATGCAAAAACAATG+AGG + chr2_3:35762343-35762362 None:intergenic 30.0%
TCAATCTTCAACATCATAGT+TGG + chr2_3:35762463-35762482 None:intergenic 30.0%
ACTATGATGTTGAAGATTGA+AGG - chr2_3:35762463-35762482 Msa0289510:intron 30.0%
!!! CAATTTTTTTTCTCCTTTGC+AGG - chr2_3:35762517-35762536 Msa0289510:exon 30.0%
AATGAAAATAGTTCCTGCAA+AGG + chr2_3:35762533-35762552 None:intergenic 30.0%
! TTGCAGGAACTATTTTCATT+TGG - chr2_3:35762533-35762552 Msa0289510:exon 30.0%
!! GTCATTTGAGCTTTGAAATA+AGG - chr2_3:35762689-35762708 Msa0289510:five_prime_UTR 30.0%
TATGTGATGCACAATTATAG+AGG - chr2_3:35762853-35762872 Msa0289510:five_prime_UTR 30.0%
ATGTGATGCACAATTATAGA+GGG - chr2_3:35762854-35762873 Msa0289510:five_prime_UTR 30.0%
TTTGGATATTGTCTATTGCA+TGG - chr2_3:35762932-35762951 Msa0289510:five_prime_UTR 30.0%
! GAAACATAAGAGTATGAGCA+TGG + chr2_3:35759020-35759039 None:intergenic 35.0%
GGAATCTAATACGATACACA+AGG - chr2_3:35759090-35759109 Msa0289510:three_prime_UTR 35.0%
TCTGTAATCACCTCATTCAT+TGG + chr2_3:35759126-35759145 None:intergenic 35.0%
TTCTCATGAGAACAAGCAAA+AGG - chr2_3:35759274-35759293 Msa0289510:intron 35.0%
ACGATATATCAAACAGCTTG+TGG + chr2_3:35759348-35759367 None:intergenic 35.0%
TGAACTCCATATAACAACGT+TGG - chr2_3:35759468-35759487 Msa0289510:intron 35.0%
GTGTAACCAACGTTGTTATA+TGG + chr2_3:35759477-35759496 None:intergenic 35.0%
! AACAACGTTGGTTACACAAT+TGG - chr2_3:35759480-35759499 Msa0289510:intron 35.0%
AATATCTGTGTGGATAGGTT+AGG - chr2_3:35759558-35759577 Msa0289510:intron 35.0%
! CAAGAAGCACATTTGCTTAT+TGG - chr2_3:35759718-35759737 Msa0289510:intron 35.0%
TAGTTTATTGTTCTGCTTGC+AGG - chr2_3:35759892-35759911 Msa0289510:intron 35.0%
CAATATGTCATGGCTTCATT+TGG - chr2_3:35759941-35759960 Msa0289510:intron 35.0%
AACATGAAGGAAGTAACATG+AGG - chr2_3:35760158-35760177 Msa0289510:intron 35.0%
ACATGAAGGAAGTAACATGA+GGG - chr2_3:35760159-35760178 Msa0289510:intron 35.0%
! ATGTTGTAGAGGATGGTTTT+TGG + chr2_3:35760201-35760220 None:intergenic 35.0%
ATTCTATCTCTGATCTGATG+TGG - chr2_3:35760292-35760311 Msa0289510:intron 35.0%
! CATGGTAACTCTCTCTTTTT+AGG - chr2_3:35760550-35760569 Msa0289510:intron 35.0%
!!! AAGCACCGACTTTTTTTGAT+GGG - chr2_3:35760697-35760716 Msa0289510:intron 35.0%
AACTTTGTGTATGTCTCATC+AGG - chr2_3:35760963-35760982 Msa0289510:intron 35.0%
! TAAAGTGCAACTTGCTTGAA+AGG - chr2_3:35761040-35761059 Msa0289510:intron 35.0%
! CTCTAAAGAGATGTCAATCA+GGG + chr2_3:35761222-35761241 None:intergenic 35.0%
CTGATTGACATCTCTTTAGA+GGG - chr2_3:35761221-35761240 Msa0289510:intron 35.0%
!! ATTTCTTTTGGGAATTGAGG+TGG - chr2_3:35761310-35761329 Msa0289510:intron 35.0%
GATTCTGCTTTCAAGAATTC+TGG + chr2_3:35761435-35761454 None:intergenic 35.0%
TCAACTACCTTCACATGATA+AGG - chr2_3:35761662-35761681 Msa0289510:intron 35.0%
TACCTTGGACTTGAAAATGA+TGG + chr2_3:35761777-35761796 None:intergenic 35.0%
GTGAATGATGAAATTTCGTG+AGG - chr2_3:35761993-35762012 Msa0289510:intron 35.0%
GAAAAAGGAAAAGGGATACA+GGG + chr2_3:35762111-35762130 None:intergenic 35.0%
AGAAAAAGGAAAAGGGATAC+AGG + chr2_3:35762112-35762131 None:intergenic 35.0%
!!! ATCCCTCATTGTTTTTGCAT+AGG - chr2_3:35762337-35762356 Msa0289510:intron 35.0%
!! AATATGGATATGATGGACAG+TGG - chr2_3:35762380-35762399 Msa0289510:intron 35.0%
CACACTTGCAAAAAATGAGA+TGG - chr2_3:35762404-35762423 Msa0289510:intron 35.0%
! TGTATTCTGCACTTTGCTAT+TGG - chr2_3:35762565-35762584 Msa0289510:five_prime_UTR 35.0%
ATGTTTGAGTAGGAAGAATG+AGG - chr2_3:35762661-35762680 Msa0289510:five_prime_UTR 35.0%
! TGATGATGTTCTCTTAAGCA+TGG - chr2_3:35762720-35762739 Msa0289510:five_prime_UTR 35.0%
GTTCTCTTAAGCATGGAATT+AGG - chr2_3:35762727-35762746 Msa0289510:five_prime_UTR 35.0%
TGTCTATTGCATGGTCTTTA+CGG - chr2_3:35762941-35762960 Msa0289510:five_prime_UTR 35.0%
AGAGAAAGAGAGGAAGATAG+AGG + chr2_3:35758836-35758855 None:intergenic 40.0%
AAAAGAAGATCAGAAGGCAG+GGG + chr2_3:35758894-35758913 None:intergenic 40.0%
GAAAAGAAGATCAGAAGGCA+GGG + chr2_3:35758895-35758914 None:intergenic 40.0%
TAGGGGAAAAGAAGATCAGA+AGG + chr2_3:35758900-35758919 None:intergenic 40.0%
AACGCTAGTGGAGTTGAAAA+GGG + chr2_3:35759045-35759064 None:intergenic 40.0%
TAGTAGAGGCAATATCTGTG+TGG - chr2_3:35759548-35759567 Msa0289510:intron 40.0%
ACTTGATACATCTTGTGCAC+TGG + chr2_3:35759691-35759710 None:intergenic 40.0%
! TGGACTTGATGACATCTACA+TGG - chr2_3:35759747-35759766 Msa0289510:intron 40.0%
AGTGCAAATGTTGTAGAGGA+TGG + chr2_3:35760208-35760227 None:intergenic 40.0%
! AGGAAGTGCAAATGTTGTAG+AGG + chr2_3:35760212-35760231 None:intergenic 40.0%
!!! GAAGCACCGACTTTTTTTGA+TGG - chr2_3:35760696-35760715 Msa0289510:intron 40.0%
TGAAGCAGAAACATGAAGAG+TGG - chr2_3:35760785-35760804 Msa0289510:intron 40.0%
! CCTCTAAAGAGATGTCAATC+AGG + chr2_3:35761223-35761242 None:intergenic 40.0%
CCTGATTGACATCTCTTTAG+AGG - chr2_3:35761220-35761239 Msa0289510:intron 40.0%
GCTGCTCAATCATAATGAAG+TGG - chr2_3:35761332-35761351 Msa0289510:intron 40.0%
! TCATAATGAAGTGGAAGCGT+AGG - chr2_3:35761341-35761360 Msa0289510:intron 40.0%
TTCTTGAACGTGGAATCAGA+GGG - chr2_3:35761388-35761407 Msa0289510:intron 40.0%
AAGCAGAATCTCGGCTTATT+AGG - chr2_3:35761445-35761464 Msa0289510:intron 40.0%
AAGTGTCTGGTAGAAGTGAT+AGG - chr2_3:35761555-35761574 Msa0289510:intron 40.0%
GTCTACAACTTTGACATGAC+AGG + chr2_3:35761725-35761744 None:intergenic 40.0%
!!! CACCATCATTTTCAAGTCCA+AGG - chr2_3:35761772-35761791 Msa0289510:intron 40.0%
!! GCTTTGGTTGGTTCTATACA+TGG - chr2_3:35762162-35762181 Msa0289510:intron 40.0%
TGCAAAAACAATGAGGGATG+AGG + chr2_3:35762336-35762355 None:intergenic 40.0%
GGAAAAGAAGATCAGAAGGC+AGG + chr2_3:35758896-35758915 None:intergenic 45.0%
! GCATTAACTTTCTGGAGGTG+AGG + chr2_3:35758987-35759006 None:intergenic 45.0%
GCATGGCATTAACTTTCTGG+AGG + chr2_3:35758992-35759011 None:intergenic 45.0%
GGAGCATGGCATTAACTTTC+TGG + chr2_3:35758995-35759014 None:intergenic 45.0%
! CATAAGAGTATGAGCATGGC+AGG + chr2_3:35759016-35759035 None:intergenic 45.0%
ACGCTAGTGGAGTTGAAAAG+GGG + chr2_3:35759044-35759063 None:intergenic 45.0%
CAACGCTAGTGGAGTTGAAA+AGG + chr2_3:35759046-35759065 None:intergenic 45.0%
TTAGATTCCCTCGCTATGTC+CGG + chr2_3:35759079-35759098 None:intergenic 45.0%
GAGGCAATATCTGTGTGGAT+AGG - chr2_3:35759553-35759572 Msa0289510:intron 45.0%
TGCTTGCAGGTAGTGTTAAG+TGG - chr2_3:35759905-35759924 Msa0289510:intron 45.0%
!! TAGCTATGGCAGTGGTTTTG+TGG + chr2_3:35760993-35761012 None:intergenic 45.0%
! GAAAGCTTTAGCTATGGCAG+TGG + chr2_3:35761001-35761020 None:intergenic 45.0%
CTTCTTGAACGTGGAATCAG+AGG - chr2_3:35761387-35761406 Msa0289510:intron 45.0%
!! AGAAGGTGCTAATGAGTGAG+AGG + chr2_3:35762204-35762223 None:intergenic 45.0%
!!! ATAACAAATTTAATTTAATA+TGG - chr2_3:35762364-35762383 Msa0289510:intron 5.0%
CAGAAGGCAGGGGATAATGA+AGG + chr2_3:35758884-35758903 None:intergenic 50.0%
AGGACAACCGAAGGAGTAAG+GGG + chr2_3:35758967-35758986 None:intergenic 50.0%
GAGGACAACCGAAGGAGTAA+GGG + chr2_3:35758968-35758987 None:intergenic 50.0%
TGAGGACAACCGAAGGAGTA+AGG + chr2_3:35758969-35758988 None:intergenic 50.0%
! TCCACTAGCGTTGAACTCGA+TGG - chr2_3:35759053-35759072 Msa0289510:three_prime_UTR 50.0%
AAGCGTAGGAGATCGAGTGA+TGG - chr2_3:35761355-35761374 Msa0289510:intron 50.0%
GTAGGAGATCGAGTGATGGT+TGG - chr2_3:35761359-35761378 Msa0289510:intron 50.0%
TTGGCAGTGCTTCTTGAACG+TGG - chr2_3:35761378-35761397 Msa0289510:intron 50.0%
ATCAGAGGGAGCAAAGACTG+TGG - chr2_3:35761402-35761421 Msa0289510:intron 50.0%
TCAGAGGGAGCAAAGACTGT+GGG - chr2_3:35761403-35761422 Msa0289510:intron 50.0%
GAGGGATGAGGAAGCCAAAA+GGG + chr2_3:35762324-35762343 None:intergenic 50.0%
TGAGGGATGAGGAAGCCAAA+AGG + chr2_3:35762325-35762344 None:intergenic 50.0%
! GTGGCAGTTTTGCAGTGAGG+AGG + chr2_3:35758945-35758964 None:intergenic 55.0%
ACTGCCACCCCTTACTCCTT+CGG - chr2_3:35758957-35758976 Msa0289510:three_prime_UTR 55.0%
ACAACCGAAGGAGTAAGGGG+TGG + chr2_3:35758964-35758983 None:intergenic 55.0%
GTATGAGCATGGCAGGAGCA+TGG + chr2_3:35759009-35759028 None:intergenic 55.0%
GCCATCGAGTTCAACGCTAG+TGG + chr2_3:35759057-35759076 None:intergenic 55.0%
! CTAGCGTTGAACTCGATGGC+CGG - chr2_3:35759057-35759076 Msa0289510:three_prime_UTR 55.0%
CAGAGGGAGCAAAGACTGTG+GGG - chr2_3:35761404-35761423 Msa0289510:intron 55.0%
AGAGGGAGCAAAGACTGTGG+GGG - chr2_3:35761405-35761424 Msa0289510:intron 55.0%
! GGGGTGGCAGTTTTGCAGTG+AGG + chr2_3:35758948-35758967 None:intergenic 60.0%
CTGGAGGTGAGGACAACCGA+AGG + chr2_3:35758976-35758995 None:intergenic 60.0%
TCGATGGCCGGACATAGCGA+GGG - chr2_3:35759069-35759088 Msa0289510:three_prime_UTR 60.0%
CTCGATGGCCGGACATAGCG+AGG - chr2_3:35759068-35759087 Msa0289510:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 35758833 35762966 35758833 ID=Msa0289510;Name=Msa0289510
chr2_3 mRNA 35758833 35762966 35758833 ID=Msa0289510-mRNA-1;Parent=Msa0289510;Name=Msa0289510-mRNA-1;_AED=0.25;_eAED=0.25;_QI=433|0.75|1|1|0|0.4|5|377|70
chr2_3 exon 35758833 35759126 35758833 ID=Msa0289510-mRNA-1:exon:11707;Parent=Msa0289510-mRNA-1
chr2_3 exon 35759211 35759260 35759211 ID=Msa0289510-mRNA-1:exon:11706;Parent=Msa0289510-mRNA-1
chr2_3 exon 35759374 35759440 35759374 ID=Msa0289510-mRNA-1:exon:11705;Parent=Msa0289510-mRNA-1
chr2_3 exon 35760006 35760154 35760006 ID=Msa0289510-mRNA-1:exon:11704;Parent=Msa0289510-mRNA-1
chr2_3 exon 35762504 35762966 35762504 ID=Msa0289510-mRNA-1:exon:11703;Parent=Msa0289510-mRNA-1
chr2_3 five_prime_UTR 35762534 35762966 35762534 ID=Msa0289510-mRNA-1:five_prime_utr;Parent=Msa0289510-mRNA-1
chr2_3 CDS 35762504 35762533 35762504 ID=Msa0289510-mRNA-1:cds;Parent=Msa0289510-mRNA-1
chr2_3 CDS 35760006 35760154 35760006 ID=Msa0289510-mRNA-1:cds;Parent=Msa0289510-mRNA-1
chr2_3 CDS 35759407 35759440 35759407 ID=Msa0289510-mRNA-1:cds;Parent=Msa0289510-mRNA-1
chr2_3 three_prime_UTR 35759374 35759406 35759374 ID=Msa0289510-mRNA-1:three_prime_utr;Parent=Msa0289510-mRNA-1
chr2_3 three_prime_UTR 35759211 35759260 35759211 ID=Msa0289510-mRNA-1:three_prime_utr;Parent=Msa0289510-mRNA-1
chr2_3 three_prime_UTR 35758833 35759126 35758833 ID=Msa0289510-mRNA-1:three_prime_utr;Parent=Msa0289510-mRNA-1
Gene Sequence

>Msa0289510

ATGGAAAATTCTCATGAGAACAAGCAAAAGGTGAGTGTATATTTTGATCAACTACCTTCACATGATAAGGATTTTTTGGACTTAATTCATAGTAAGACTATTGGATTCCTGTCATGTCAAAGTTGTAGACTGCTTCAATTTCAAGCTACTTCTAAGGCACCATCATTTTCAAGTCCAAGGATATATAACAAATTTAATTTAATATGGATATGA
Protein sequence

>Msa0289510

MENSHENKQKVSVYFDQLPSHDKDFLDLIHSKTIGFLSCQSCRLLQFQATSKAPSFSSPRIYNKFNLIWI*