AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0113250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0113250 MtrunA17_Chr7g0246631 50.000 76 34 1 5 80 147 218 8.38e-15 68.2
Msa0113250 MtrunA17_Chr8g0350321 47.368 76 36 1 5 80 171 242 5.50e-14 65.9
Msa0113250 MtrunA17_Chr6g0472471 44.444 81 39 2 5 85 125 199 7.31e-13 62.8
Msa0113250 MtrunA17_Chr1g0203671 42.105 76 40 1 5 80 151 222 2.87e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0113250 AT4G00730.1 44.737 76 38 1 5 80 190 261 7.89e-14 65.9
Msa0113250 AT4G00730.2 43.750 80 41 1 5 84 190 265 1.22e-13 65.1
Msa0113250 AT3G61150.1 44.737 76 38 1 5 80 166 237 1.88e-13 64.7

Find 49 sgRNAs with CRISPR-Local

Find 279 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAACACCTCAAAACCAAAT+TGG 0.213419 1_3:+29023797 None:intergenic
CCCGTCTCTTGTTCCTTTGT+CGG 0.248912 1_3:+29022513 None:intergenic
CCACTGTGTGGCTCCGATTC+TGG 0.251694 1_3:-29023665 Msa0113250:five_prime_UTR
ACTAACAGAATATCCCAATT+TGG 0.258384 1_3:-29023811 Msa0113250:five_prime_UTR
TGTTTGTCTGAGATCGTTCC+TGG 0.299666 1_3:-29023263 Msa0113250:five_prime_UTR
AGTCAGAGAAGTTTGAAATT+TGG 0.314997 1_3:-29023473 Msa0113250:five_prime_UTR
TGGTTGAAATTGCTATAAAT+AGG 0.360277 1_3:-29023359 Msa0113250:five_prime_UTR
AATTGCTATAAATAGGTGTT+TGG 0.374168 1_3:-29023352 Msa0113250:five_prime_UTR
TCTGACTACAAAATCAAGAT+TGG 0.384014 1_3:+29023489 None:intergenic
ATGCGTGGAGGATAAGAATT+CGG 0.385616 1_3:-29023235 Msa0113250:five_prime_UTR
TCAAGAGAATATGTCTAATC+AGG 0.386275 1_3:+29023191 None:intergenic
TGTCTAATCAGGATTCAAAA+AGG 0.390470 1_3:+29023202 None:intergenic
TGTGAATGAAATTTAGCATC+AGG 0.395238 1_3:-29023753 Msa0113250:five_prime_UTR
TGCGTGGAGGATAAGAATTC+GGG 0.431135 1_3:-29023234 Msa0113250:five_prime_UTR
AAACACCTCAAAACCAAATT+GGG 0.433895 1_3:+29023798 None:intergenic
GAAATCTCACTAATAATAGT+TGG 0.456589 1_3:+29021686 None:intergenic
AAAACGCCATGCACCAGAAT+CGG 0.472982 1_3:+29023652 None:intergenic
AATTGAAAATGCTAGGTTGA+AGG 0.481602 1_3:-29021642 Msa0113250:CDS
ATCCTCCACGCATCTTTCCC+AGG 0.486423 1_3:+29023245 None:intergenic
CTGACTACAAAATCAAGATT+GGG 0.509068 1_3:+29023490 None:intergenic
TCCGACAAAGGAACAAGAGA+CGG 0.523267 1_3:-29022514 Msa0113250:CDS
TTGTCACAATTTGAACATGT+AGG 0.526784 1_3:+29021710 None:intergenic
GTGAATGAAATTTAGCATCA+GGG 0.528621 1_3:-29023752 Msa0113250:five_prime_UTR
GTGGCTCCGATTCTGGTGCA+TGG 0.529383 1_3:-29023658 Msa0113250:five_prime_UTR
AGAGAGTCGACAAGAGCAAG+AGG 0.547069 1_3:-29022556 Msa0113250:CDS
TCAATGTTTCCTGACAGCAT+TGG 0.557881 1_3:+29023439 None:intergenic
TTAGACATATTCTCTTGAGA+TGG 0.560674 1_3:-29023186 Msa0113250:five_prime_UTR
GATCTCAGACAAACAATCAA+AGG 0.567322 1_3:+29023271 None:intergenic
GTTTGTCTGAGATCGTTCCT+GGG 0.581994 1_3:-29023262 Msa0113250:five_prime_UTR
GTCTTCAAATTGAAAATGCT+AGG 0.584052 1_3:-29021649 Msa0113250:CDS
CCGACAAAGGAACAAGAGAC+GGG 0.589475 1_3:-29022513 Msa0113250:CDS
CTTATTTGATGAGATTGAGA+TGG 0.598380 1_3:-29021582 Msa0113250:CDS
TGGTGAAGGCAAGAAACAGA+AGG 0.611876 1_3:-29021562 Msa0113250:CDS
GATGGTGCTCATCGATGAGA+AGG 0.622187 1_3:-29023168 Msa0113250:intron
TGAATGAAATTTAGCATCAG+GGG 0.622534 1_3:-29023751 Msa0113250:five_prime_UTR
TGATGAGATTGAGATGGTGA+AGG 0.630063 1_3:-29021576 Msa0113250:CDS
TCGTTCCTGGGAAAGATGCG+TGG 0.643650 1_3:-29023250 Msa0113250:five_prime_UTR
TTGATTGAAATTCCACTGTG+TGG 0.649168 1_3:-29023677 Msa0113250:five_prime_UTR
AACAAGAGACGGGAAAACAC+CGG 0.651664 1_3:-29022503 Msa0113250:intron
GGAACTGCCACAAGGTATCG+AGG 0.659979 1_3:-29023558 Msa0113250:five_prime_UTR
AAAAGAGTACAATCCGACAA+AGG 0.664827 1_3:-29022526 Msa0113250:CDS
GGTGAAGGCAAGAAACAGAA+GGG 0.672932 1_3:-29021561 None:intergenic
ACGGAAAACTCATTTGACGA+AGG 0.684179 1_3:-29022589 Msa0113250:CDS
CAAACTACCTCGATACCTTG+TGG 0.686876 1_3:+29023551 None:intergenic
TTCCTGGGAAAGATGCGTGG+AGG 0.702740 1_3:-29023247 Msa0113250:five_prime_UTR
AGACAGAGCTGCTGACACGA+AGG 0.703158 1_3:+29023408 None:intergenic
ATTATGTGGGAACTGCCACA+AGG 0.704501 1_3:-29023566 Msa0113250:five_prime_UTR
GTGGAGGATAAGAATTCGGG+AGG 0.705534 1_3:-29023231 Msa0113250:five_prime_UTR
CCAGAATCGGAGCCACACAG+TGG 0.733158 1_3:+29023665 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACAAACAATTTTTTTAAAAA+AGG + chr1_3:29022731-29022750 None:intergenic 10.0%
!!! TTGTTATTTGTTTTTTTTTT+GGG - chr1_3:29023237-29023256 Msa0113250:five_prime_UTR 10.0%
!!! ACAAACAATTTTTTTAAAAA+AGG + chr1_3:29022731-29022750 None:intergenic 10.0%
!!! TTGTTATTTGTTTTTTTTTT+GGG - chr1_3:29023237-29023256 Msa0113250:five_prime_UTR 10.0%
!!! GTTGTTATTTGTTTTTTTTT+TGG - chr1_3:29023236-29023255 Msa0113250:five_prime_UTR 15.0%
!! TTAAATCTGAAAAAAATAGT+AGG + chr1_3:29023380-29023399 None:intergenic 15.0%
!!! TAATATGTTTCAGATATTTT+TGG - chr1_3:29023413-29023432 Msa0113250:five_prime_UTR 15.0%
!! AGTATATAATATCTTTATGA+TGG - chr1_3:29023632-29023651 Msa0113250:five_prime_UTR 15.0%
!!! GTTGTTATTTGTTTTTTTTT+TGG - chr1_3:29023236-29023255 Msa0113250:five_prime_UTR 15.0%
!! TTAAATCTGAAAAAAATAGT+AGG + chr1_3:29023380-29023399 None:intergenic 15.0%
!!! TAATATGTTTCAGATATTTT+TGG - chr1_3:29023413-29023432 Msa0113250:five_prime_UTR 15.0%
!! AGTATATAATATCTTTATGA+TGG - chr1_3:29023632-29023651 Msa0113250:five_prime_UTR 15.0%
!!! TTGTTTTTGATCAAATTATG+TGG - chr1_3:29021798-29021817 Msa0113250:intron 20.0%
!!! TGTTTTTGATCAAATTATGT+GGG - chr1_3:29021799-29021818 Msa0113250:intron 20.0%
!!! GAAATCTGTTTTTGAATTTA+TGG - chr1_3:29022301-29022320 Msa0113250:intron 20.0%
!!! GGATAATTTAAAACAGAATA+GGG + chr1_3:29022553-29022572 None:intergenic 20.0%
!!! TGGATAATTTAAAACAGAAT+AGG + chr1_3:29022554-29022573 None:intergenic 20.0%
!!! TAGACTGAGTTATTTTTTAT+TGG - chr1_3:29022694-29022713 Msa0113250:intron 20.0%
!!! AAATATAAAGAAGGTATTGT+TGG + chr1_3:29022966-29022985 None:intergenic 20.0%
!! TCTATACCTTAAAATAATGT+GGG + chr1_3:29023081-29023100 None:intergenic 20.0%
!! TTCTATACCTTAAAATAATG+TGG + chr1_3:29023082-29023101 None:intergenic 20.0%
!!! TTTAGAGGTATTGTTAATAT+TGG - chr1_3:29023181-29023200 Msa0113250:five_prime_UTR 20.0%
!!! TTTTTTTTTTGGGAATGAAA+TGG - chr1_3:29023247-29023266 Msa0113250:five_prime_UTR 20.0%
!! AAGCTATTATTTGGAAAATA+AGG + chr1_3:29023298-29023317 None:intergenic 20.0%
!! TAAAGTTTAGAAAAATTGTG+AGG + chr1_3:29023327-29023346 None:intergenic 20.0%
!!! CATTTGTGTTATCTTTTTAA+TGG + chr1_3:29023542-29023561 None:intergenic 20.0%
!!! AAGTTGATATCATTTTTACA+TGG - chr1_3:29023563-29023582 Msa0113250:five_prime_UTR 20.0%
!!! TATTATATACTCTAAAGCTT+AGG + chr1_3:29023623-29023642 None:intergenic 20.0%
!!! TTGTTTTTGATCAAATTATG+TGG - chr1_3:29021798-29021817 Msa0113250:intron 20.0%
!!! TGTTTTTGATCAAATTATGT+GGG - chr1_3:29021799-29021818 Msa0113250:intron 20.0%
!!! GAAATCTGTTTTTGAATTTA+TGG - chr1_3:29022301-29022320 Msa0113250:intron 20.0%
!!! GGATAATTTAAAACAGAATA+GGG + chr1_3:29022553-29022572 None:intergenic 20.0%
!!! TGGATAATTTAAAACAGAAT+AGG + chr1_3:29022554-29022573 None:intergenic 20.0%
!!! TAGACTGAGTTATTTTTTAT+TGG - chr1_3:29022694-29022713 Msa0113250:intron 20.0%
!!! AAATATAAAGAAGGTATTGT+TGG + chr1_3:29022966-29022985 None:intergenic 20.0%
!! TCTATACCTTAAAATAATGT+GGG + chr1_3:29023081-29023100 None:intergenic 20.0%
!! TTCTATACCTTAAAATAATG+TGG + chr1_3:29023082-29023101 None:intergenic 20.0%
!!! TTTAGAGGTATTGTTAATAT+TGG - chr1_3:29023181-29023200 Msa0113250:five_prime_UTR 20.0%
!!! TTTTTTTTTTGGGAATGAAA+TGG - chr1_3:29023247-29023266 Msa0113250:five_prime_UTR 20.0%
!! AAGCTATTATTTGGAAAATA+AGG + chr1_3:29023298-29023317 None:intergenic 20.0%
!! TAAAGTTTAGAAAAATTGTG+AGG + chr1_3:29023327-29023346 None:intergenic 20.0%
!!! CATTTGTGTTATCTTTTTAA+TGG + chr1_3:29023542-29023561 None:intergenic 20.0%
!!! AAGTTGATATCATTTTTACA+TGG - chr1_3:29023563-29023582 Msa0113250:five_prime_UTR 20.0%
!!! TATTATATACTCTAAAGCTT+AGG + chr1_3:29023623-29023642 None:intergenic 20.0%
!!! CATTTTTCAATGGAGATTTT+GGG + chr1_3:29021860-29021879 None:intergenic 25.0%
!!! TCATTTTTCAATGGAGATTT+TGG + chr1_3:29021861-29021880 None:intergenic 25.0%
! TGGTTGAAATTGCTATAAAT+AGG - chr1_3:29022019-29022038 Msa0113250:intron 25.0%
! AATTGCTATAAATAGGTGTT+TGG - chr1_3:29022026-29022045 Msa0113250:intron 25.0%
! ATTTATCAATCTTTGTATGC+AGG + chr1_3:29022503-29022522 None:intergenic 25.0%
! ATGTGATTTATACTTGAAGA+AGG + chr1_3:29022591-29022610 None:intergenic 25.0%
!! ATTGTTTTCTTTGTTGAAGA+CGG - chr1_3:29022770-29022789 Msa0113250:intron 25.0%
! ACCTTCTTTATATTTAGTTG+TGG - chr1_3:29022971-29022990 Msa0113250:intron 25.0%
! ACCACAACTAAATATAAAGA+AGG + chr1_3:29022975-29022994 None:intergenic 25.0%
!! GCTAAATTTTACTTCCAAAA+GGG + chr1_3:29023161-29023180 None:intergenic 25.0%
!! AGCTAAATTTTACTTCCAAA+AGG + chr1_3:29023162-29023181 None:intergenic 25.0%
!!! GAAGTAAAATTTAGCTTTAG+AGG - chr1_3:29023166-29023185 Msa0113250:intron 25.0%
! ATAAACATGTCAATTGTTAG+GGG + chr1_3:29023209-29023228 None:intergenic 25.0%
! CATAAACATGTCAATTGTTA+GGG + chr1_3:29023210-29023229 None:intergenic 25.0%
! ACATAAACATGTCAATTGTT+AGG + chr1_3:29023211-29023230 None:intergenic 25.0%
! ACAATTGACATGTTTATGTT+CGG - chr1_3:29023212-29023231 Msa0113250:five_prime_UTR 25.0%
! AGGAAAGATAAGCTATTATT+TGG + chr1_3:29023307-29023326 None:intergenic 25.0%
! ATTAAGAGTAAAGGTGAAAA+TGG + chr1_3:29023352-29023371 None:intergenic 25.0%
! TAGGATGAAATTAAGAGTAA+AGG + chr1_3:29023361-29023380 None:intergenic 25.0%
!!! TTTACATGGTTTTATCTTTC+TGG - chr1_3:29023577-29023596 Msa0113250:five_prime_UTR 25.0%
! GAAATCTCACTAATAATAGT+TGG + chr1_3:29023695-29023714 None:intergenic 25.0%
!! TAGCATTTTCAATTTGAAGA+CGG + chr1_3:29023730-29023749 None:intergenic 25.0%
!!! CATTTTTCAATGGAGATTTT+GGG + chr1_3:29021860-29021879 None:intergenic 25.0%
!!! TCATTTTTCAATGGAGATTT+TGG + chr1_3:29021861-29021880 None:intergenic 25.0%
! TGGTTGAAATTGCTATAAAT+AGG - chr1_3:29022019-29022038 Msa0113250:intron 25.0%
! AATTGCTATAAATAGGTGTT+TGG - chr1_3:29022026-29022045 Msa0113250:intron 25.0%
! ATTTATCAATCTTTGTATGC+AGG + chr1_3:29022503-29022522 None:intergenic 25.0%
! ATGTGATTTATACTTGAAGA+AGG + chr1_3:29022591-29022610 None:intergenic 25.0%
!! ATTGTTTTCTTTGTTGAAGA+CGG - chr1_3:29022770-29022789 Msa0113250:intron 25.0%
! ACCTTCTTTATATTTAGTTG+TGG - chr1_3:29022971-29022990 Msa0113250:intron 25.0%
! ACCACAACTAAATATAAAGA+AGG + chr1_3:29022975-29022994 None:intergenic 25.0%
!! GCTAAATTTTACTTCCAAAA+GGG + chr1_3:29023161-29023180 None:intergenic 25.0%
!! AGCTAAATTTTACTTCCAAA+AGG + chr1_3:29023162-29023181 None:intergenic 25.0%
!!! GAAGTAAAATTTAGCTTTAG+AGG - chr1_3:29023166-29023185 Msa0113250:intron 25.0%
! ATAAACATGTCAATTGTTAG+GGG + chr1_3:29023209-29023228 None:intergenic 25.0%
! CATAAACATGTCAATTGTTA+GGG + chr1_3:29023210-29023229 None:intergenic 25.0%
! ACATAAACATGTCAATTGTT+AGG + chr1_3:29023211-29023230 None:intergenic 25.0%
! ACAATTGACATGTTTATGTT+CGG - chr1_3:29023212-29023231 Msa0113250:five_prime_UTR 25.0%
! AGGAAAGATAAGCTATTATT+TGG + chr1_3:29023307-29023326 None:intergenic 25.0%
! ATTAAGAGTAAAGGTGAAAA+TGG + chr1_3:29023352-29023371 None:intergenic 25.0%
! TAGGATGAAATTAAGAGTAA+AGG + chr1_3:29023361-29023380 None:intergenic 25.0%
!!! TTTACATGGTTTTATCTTTC+TGG - chr1_3:29023577-29023596 Msa0113250:five_prime_UTR 25.0%
! GAAATCTCACTAATAATAGT+TGG + chr1_3:29023695-29023714 None:intergenic 25.0%
!! TAGCATTTTCAATTTGAAGA+CGG + chr1_3:29023730-29023749 None:intergenic 25.0%
ACTAACAGAATATCCCAATT+TGG - chr1_3:29021567-29021586 Msa0113250:CDS 30.0%
!!! AATATCCCAATTTGGTTTTG+AGG - chr1_3:29021575-29021594 Msa0113250:CDS 30.0%
AAACACCTCAAAACCAAATT+GGG + chr1_3:29021583-29021602 None:intergenic 30.0%
TAAACACCTCAAAACCAAAT+TGG + chr1_3:29021584-29021603 None:intergenic 30.0%
TGTGAATGAAATTTAGCATC+AGG - chr1_3:29021625-29021644 Msa0113250:CDS 30.0%
GTGAATGAAATTTAGCATCA+GGG - chr1_3:29021626-29021645 Msa0113250:CDS 30.0%
TGAATGAAATTTAGCATCAG+GGG - chr1_3:29021627-29021646 Msa0113250:CDS 30.0%
!!! GCGTTTTTTCTCTAATTTTC+TGG - chr1_3:29021742-29021761 Msa0113250:intron 30.0%
CTCCATTGAAAAATGAAATG+CGG - chr1_3:29021865-29021884 Msa0113250:intron 30.0%
CTGACTACAAAATCAAGATT+GGG + chr1_3:29021891-29021910 None:intergenic 30.0%
TCTGACTACAAAATCAAGAT+TGG + chr1_3:29021892-29021911 None:intergenic 30.0%
! AGTCAGAGAAGTTTGAAATT+TGG - chr1_3:29021905-29021924 Msa0113250:intron 30.0%
AGAGAAAAACAATGGCAATT+CGG + chr1_3:29022000-29022019 None:intergenic 30.0%
TTTCAACCAGAGAAAAACAA+TGG + chr1_3:29022008-29022027 None:intergenic 30.0%
TAATCAGGATTCAAAAAGGT+TGG + chr1_3:29022175-29022194 None:intergenic 30.0%
TGTCTAATCAGGATTCAAAA+AGG + chr1_3:29022179-29022198 None:intergenic 30.0%
TCAAGAGAATATGTCTAATC+AGG + chr1_3:29022190-29022209 None:intergenic 30.0%
TTAGACATATTCTCTTGAGA+TGG - chr1_3:29022192-29022211 Msa0113250:intron 30.0%
! TTTGAAACAAAACATGAGCA+AGG + chr1_3:29022264-29022283 None:intergenic 30.0%
!! TGGTCGTTTTGTTATGATTT+TGG - chr1_3:29022321-29022340 Msa0113250:intron 30.0%
!! TATGATTTTGGTGAATTCGT+AGG - chr1_3:29022333-29022352 Msa0113250:intron 30.0%
!! ATGATTTTGGTGAATTCGTA+GGG - chr1_3:29022334-29022353 Msa0113250:intron 30.0%
AATCTTTGTATGCAGGATAT+GGG + chr1_3:29022496-29022515 None:intergenic 30.0%
!! TTTTCGGTCAGTTTGATTTA+AGG - chr1_3:29022904-29022923 Msa0113250:intron 30.0%
! TTTCGGTCAGTTTGATTTAA+GGG - chr1_3:29022905-29022924 Msa0113250:intron 30.0%
!! GTTGTTCCCACATTATTTTA+AGG - chr1_3:29023072-29023091 Msa0113250:intron 30.0%
AATGAAATGGTTTATCACCA+AGG - chr1_3:29023260-29023279 Msa0113250:five_prime_UTR 30.0%
!!! TGTTTCAGATATTTTTGGCA+AGG - chr1_3:29023418-29023437 Msa0113250:five_prime_UTR 30.0%
!! ATCTTTATGATGGATGCAAT+AGG - chr1_3:29023642-29023661 Msa0113250:five_prime_UTR 30.0%
TTGTCACAATTTGAACATGT+AGG + chr1_3:29023671-29023690 None:intergenic 30.0%
GTCTTCAAATTGAAAATGCT+AGG - chr1_3:29023729-29023748 Msa0113250:five_prime_UTR 30.0%
! AATTGAAAATGCTAGGTTGA+AGG - chr1_3:29023736-29023755 Msa0113250:five_prime_UTR 30.0%
CTTATTTGATGAGATTGAGA+TGG - chr1_3:29023796-29023815 Msa0113250:five_prime_UTR 30.0%
ACTAACAGAATATCCCAATT+TGG - chr1_3:29021567-29021586 Msa0113250:CDS 30.0%
!!! AATATCCCAATTTGGTTTTG+AGG - chr1_3:29021575-29021594 Msa0113250:CDS 30.0%
AAACACCTCAAAACCAAATT+GGG + chr1_3:29021583-29021602 None:intergenic 30.0%
TAAACACCTCAAAACCAAAT+TGG + chr1_3:29021584-29021603 None:intergenic 30.0%
TGTGAATGAAATTTAGCATC+AGG - chr1_3:29021625-29021644 Msa0113250:CDS 30.0%
GTGAATGAAATTTAGCATCA+GGG - chr1_3:29021626-29021645 Msa0113250:CDS 30.0%
TGAATGAAATTTAGCATCAG+GGG - chr1_3:29021627-29021646 Msa0113250:CDS 30.0%
!!! GCGTTTTTTCTCTAATTTTC+TGG - chr1_3:29021742-29021761 Msa0113250:intron 30.0%
CTCCATTGAAAAATGAAATG+CGG - chr1_3:29021865-29021884 Msa0113250:intron 30.0%
CTGACTACAAAATCAAGATT+GGG + chr1_3:29021891-29021910 None:intergenic 30.0%
TCTGACTACAAAATCAAGAT+TGG + chr1_3:29021892-29021911 None:intergenic 30.0%
! AGTCAGAGAAGTTTGAAATT+TGG - chr1_3:29021905-29021924 Msa0113250:intron 30.0%
AGAGAAAAACAATGGCAATT+CGG + chr1_3:29022000-29022019 None:intergenic 30.0%
TTTCAACCAGAGAAAAACAA+TGG + chr1_3:29022008-29022027 None:intergenic 30.0%
TAATCAGGATTCAAAAAGGT+TGG + chr1_3:29022175-29022194 None:intergenic 30.0%
TGTCTAATCAGGATTCAAAA+AGG + chr1_3:29022179-29022198 None:intergenic 30.0%
TCAAGAGAATATGTCTAATC+AGG + chr1_3:29022190-29022209 None:intergenic 30.0%
TTAGACATATTCTCTTGAGA+TGG - chr1_3:29022192-29022211 Msa0113250:intron 30.0%
! TTTGAAACAAAACATGAGCA+AGG + chr1_3:29022264-29022283 None:intergenic 30.0%
!! TGGTCGTTTTGTTATGATTT+TGG - chr1_3:29022321-29022340 Msa0113250:intron 30.0%
!! TATGATTTTGGTGAATTCGT+AGG - chr1_3:29022333-29022352 Msa0113250:intron 30.0%
!! ATGATTTTGGTGAATTCGTA+GGG - chr1_3:29022334-29022353 Msa0113250:intron 30.0%
AATCTTTGTATGCAGGATAT+GGG + chr1_3:29022496-29022515 None:intergenic 30.0%
!! TTTTCGGTCAGTTTGATTTA+AGG - chr1_3:29022904-29022923 Msa0113250:intron 30.0%
! TTTCGGTCAGTTTGATTTAA+GGG - chr1_3:29022905-29022924 Msa0113250:intron 30.0%
!! GTTGTTCCCACATTATTTTA+AGG - chr1_3:29023072-29023091 Msa0113250:intron 30.0%
AATGAAATGGTTTATCACCA+AGG - chr1_3:29023260-29023279 Msa0113250:five_prime_UTR 30.0%
!!! TGTTTCAGATATTTTTGGCA+AGG - chr1_3:29023418-29023437 Msa0113250:five_prime_UTR 30.0%
!! ATCTTTATGATGGATGCAAT+AGG - chr1_3:29023642-29023661 Msa0113250:five_prime_UTR 30.0%
TTGTCACAATTTGAACATGT+AGG + chr1_3:29023671-29023690 None:intergenic 30.0%
GTCTTCAAATTGAAAATGCT+AGG - chr1_3:29023729-29023748 Msa0113250:five_prime_UTR 30.0%
! AATTGAAAATGCTAGGTTGA+AGG - chr1_3:29023736-29023755 Msa0113250:five_prime_UTR 30.0%
CTTATTTGATGAGATTGAGA+TGG - chr1_3:29023796-29023815 Msa0113250:five_prime_UTR 30.0%
TTGATTGAAATTCCACTGTG+TGG - chr1_3:29021701-29021720 Msa0113250:CDS 35.0%
!! GAATTGCCATTGTTTTTCTC+TGG - chr1_3:29021999-29022018 Msa0113250:intron 35.0%
GATCTCAGACAAACAATCAA+AGG + chr1_3:29022110-29022129 None:intergenic 35.0%
AAAAAGAGCAACATCAAACC+TGG + chr1_3:29022419-29022438 None:intergenic 35.0%
CAATCTTTGTATGCAGGATA+TGG + chr1_3:29022497-29022516 None:intergenic 35.0%
TGAAGAAGGACAAATATGAG+AGG + chr1_3:29022577-29022596 None:intergenic 35.0%
AGATTTCAACACTGAATGGA+TGG + chr1_3:29022648-29022667 None:intergenic 35.0%
ATCGAGATTTCAACACTGAA+TGG + chr1_3:29022652-29022671 None:intergenic 35.0%
! AAAAGAGTACAATCCGACAA+AGG - chr1_3:29022852-29022871 Msa0113250:intron 35.0%
! AGGTATTGTTGGAGAAGAAA+GGG + chr1_3:29022955-29022974 None:intergenic 35.0%
!! AAGGTATTGTTGGAGAAGAA+AGG + chr1_3:29022956-29022975 None:intergenic 35.0%
CTATGACTAGAGGAACTATT+GGG - chr1_3:29023022-29023041 Msa0113250:intron 35.0%
! CTTTCATCAAATAGCCCTTT+TGG - chr1_3:29023144-29023163 Msa0113250:intron 35.0%
!!! GATATTTTTGGCAAGGATCA+TGG - chr1_3:29023425-29023444 Msa0113250:five_prime_UTR 35.0%
CAATCTCTCTTTAACTCAGA+AGG + chr1_3:29023464-29023483 None:intergenic 35.0%
CTGAGTTAAAGAGAGATTGT+CGG - chr1_3:29023465-29023484 Msa0113250:five_prime_UTR 35.0%
ATTGTCGGAGATTCGTATAA+CGG - chr1_3:29023480-29023499 Msa0113250:five_prime_UTR 35.0%
TTGATTGAAATTCCACTGTG+TGG - chr1_3:29021701-29021720 Msa0113250:CDS 35.0%
!! GAATTGCCATTGTTTTTCTC+TGG - chr1_3:29021999-29022018 Msa0113250:intron 35.0%
GATCTCAGACAAACAATCAA+AGG + chr1_3:29022110-29022129 None:intergenic 35.0%
AAAAAGAGCAACATCAAACC+TGG + chr1_3:29022419-29022438 None:intergenic 35.0%
CAATCTTTGTATGCAGGATA+TGG + chr1_3:29022497-29022516 None:intergenic 35.0%
TGAAGAAGGACAAATATGAG+AGG + chr1_3:29022577-29022596 None:intergenic 35.0%
AGATTTCAACACTGAATGGA+TGG + chr1_3:29022648-29022667 None:intergenic 35.0%
ATCGAGATTTCAACACTGAA+TGG + chr1_3:29022652-29022671 None:intergenic 35.0%
! AAAAGAGTACAATCCGACAA+AGG - chr1_3:29022852-29022871 Msa0113250:intron 35.0%
! AGGTATTGTTGGAGAAGAAA+GGG + chr1_3:29022955-29022974 None:intergenic 35.0%
!! AAGGTATTGTTGGAGAAGAA+AGG + chr1_3:29022956-29022975 None:intergenic 35.0%
CTATGACTAGAGGAACTATT+GGG - chr1_3:29023022-29023041 Msa0113250:intron 35.0%
! CTTTCATCAAATAGCCCTTT+TGG - chr1_3:29023144-29023163 Msa0113250:intron 35.0%
!!! GATATTTTTGGCAAGGATCA+TGG - chr1_3:29023425-29023444 Msa0113250:five_prime_UTR 35.0%
CAATCTCTCTTTAACTCAGA+AGG + chr1_3:29023464-29023483 None:intergenic 35.0%
CTGAGTTAAAGAGAGATTGT+CGG - chr1_3:29023465-29023484 Msa0113250:five_prime_UTR 35.0%
ATTGTCGGAGATTCGTATAA+CGG - chr1_3:29023480-29023499 Msa0113250:five_prime_UTR 35.0%
!! GGCCGCATTTCATTTTTCAA+TGG + chr1_3:29021870-29021889 None:intergenic 40.0%
TCAATGTTTCCTGACAGCAT+TGG + chr1_3:29021942-29021961 None:intergenic 40.0%
ATGCGTGGAGGATAAGAATT+CGG - chr1_3:29022143-29022162 Msa0113250:intron 40.0%
TTGTATGCAGGATATGGGTT+GGG + chr1_3:29022491-29022510 None:intergenic 40.0%
TTTGTATGCAGGATATGGGT+TGG + chr1_3:29022492-29022511 None:intergenic 40.0%
!! ACAGAATAGGGTGTTGAACT+TGG + chr1_3:29022541-29022560 None:intergenic 40.0%
AGAAGGACAAATATGAGAGG+TGG + chr1_3:29022574-29022593 None:intergenic 40.0%
ACGGAAAACTCATTTGACGA+AGG - chr1_3:29022789-29022808 Msa0113250:intron 40.0%
! AAAACACCGGTGGAGTTTTT+CGG - chr1_3:29022888-29022907 Msa0113250:intron 40.0%
GGTATTGTTGGAGAAGAAAG+GGG + chr1_3:29022954-29022973 None:intergenic 40.0%
AATGCTTCTGCTATGACTAG+AGG - chr1_3:29023012-29023031 Msa0113250:intron 40.0%
GCTATGACTAGAGGAACTAT+TGG - chr1_3:29023021-29023040 Msa0113250:intron 40.0%
AATGGTTTATCACCAAGGCA+AGG - chr1_3:29023265-29023284 Msa0113250:five_prime_UTR 40.0%
TTGTCGGAGATTCGTATAAC+GGG - chr1_3:29023481-29023500 Msa0113250:five_prime_UTR 40.0%
TGATGAGATTGAGATGGTGA+AGG - chr1_3:29023802-29023821 Msa0113250:five_prime_UTR 40.0%
!! GGCCGCATTTCATTTTTCAA+TGG + chr1_3:29021870-29021889 None:intergenic 40.0%
TCAATGTTTCCTGACAGCAT+TGG + chr1_3:29021942-29021961 None:intergenic 40.0%
ATGCGTGGAGGATAAGAATT+CGG - chr1_3:29022143-29022162 Msa0113250:intron 40.0%
TTGTATGCAGGATATGGGTT+GGG + chr1_3:29022491-29022510 None:intergenic 40.0%
TTTGTATGCAGGATATGGGT+TGG + chr1_3:29022492-29022511 None:intergenic 40.0%
!! ACAGAATAGGGTGTTGAACT+TGG + chr1_3:29022541-29022560 None:intergenic 40.0%
AGAAGGACAAATATGAGAGG+TGG + chr1_3:29022574-29022593 None:intergenic 40.0%
ACGGAAAACTCATTTGACGA+AGG - chr1_3:29022789-29022808 Msa0113250:intron 40.0%
! AAAACACCGGTGGAGTTTTT+CGG - chr1_3:29022888-29022907 Msa0113250:intron 40.0%
GGTATTGTTGGAGAAGAAAG+GGG + chr1_3:29022954-29022973 None:intergenic 40.0%
AATGCTTCTGCTATGACTAG+AGG - chr1_3:29023012-29023031 Msa0113250:intron 40.0%
GCTATGACTAGAGGAACTAT+TGG - chr1_3:29023021-29023040 Msa0113250:intron 40.0%
AATGGTTTATCACCAAGGCA+AGG - chr1_3:29023265-29023284 Msa0113250:five_prime_UTR 40.0%
TTGTCGGAGATTCGTATAAC+GGG - chr1_3:29023481-29023500 Msa0113250:five_prime_UTR 40.0%
TGATGAGATTGAGATGGTGA+AGG - chr1_3:29023802-29023821 Msa0113250:five_prime_UTR 40.0%
AAAACGCCATGCACCAGAAT+CGG + chr1_3:29021729-29021748 None:intergenic 45.0%
ATTATGTGGGAACTGCCACA+AGG - chr1_3:29021812-29021831 Msa0113250:intron 45.0%
CAAACTACCTCGATACCTTG+TGG + chr1_3:29021830-29021849 None:intergenic 45.0%
! GATTTTGTGCCAATGCTGTC+AGG - chr1_3:29021930-29021949 Msa0113250:intron 45.0%
! TGTTTGTCTGAGATCGTTCC+TGG - chr1_3:29022115-29022134 Msa0113250:intron 45.0%
! GTTTGTCTGAGATCGTTCCT+GGG - chr1_3:29022116-29022135 Msa0113250:intron 45.0%
TGCGTGGAGGATAAGAATTC+GGG - chr1_3:29022144-29022163 Msa0113250:intron 45.0%
TCAACATTAGCATCACCACC+AGG - chr1_3:29022398-29022417 Msa0113250:intron 45.0%
AAGAGCAACATCAAACCTGG+TGG + chr1_3:29022416-29022435 None:intergenic 45.0%
!! CAGGATATGGGTTGGGTTTA+TGG + chr1_3:29022484-29022503 None:intergenic 45.0%
TCCGACAAAGGAACAAGAGA+CGG - chr1_3:29022864-29022883 Msa0113250:intron 45.0%
AACAAGAGACGGGAAAACAC+CGG - chr1_3:29022875-29022894 Msa0113250:intron 45.0%
AACTGACCGAAAAACTCCAC+CGG + chr1_3:29022897-29022916 None:intergenic 45.0%
GGAACTATTGGGTTTGTGCA+TGG - chr1_3:29023033-29023052 Msa0113250:intron 45.0%
TTTATCACCAAGGCAAGGAC+TGG - chr1_3:29023270-29023289 Msa0113250:five_prime_UTR 45.0%
TGGTGAAGGCAAGAAACAGA+AGG - chr1_3:29023816-29023835 Msa0113250:five_prime_UTR 45.0%
AAAACGCCATGCACCAGAAT+CGG + chr1_3:29021729-29021748 None:intergenic 45.0%
ATTATGTGGGAACTGCCACA+AGG - chr1_3:29021812-29021831 Msa0113250:intron 45.0%
CAAACTACCTCGATACCTTG+TGG + chr1_3:29021830-29021849 None:intergenic 45.0%
! GATTTTGTGCCAATGCTGTC+AGG - chr1_3:29021930-29021949 Msa0113250:intron 45.0%
! TGTTTGTCTGAGATCGTTCC+TGG - chr1_3:29022115-29022134 Msa0113250:intron 45.0%
! GTTTGTCTGAGATCGTTCCT+GGG - chr1_3:29022116-29022135 Msa0113250:intron 45.0%
TGCGTGGAGGATAAGAATTC+GGG - chr1_3:29022144-29022163 Msa0113250:intron 45.0%
TCAACATTAGCATCACCACC+AGG - chr1_3:29022398-29022417 Msa0113250:intron 45.0%
AAGAGCAACATCAAACCTGG+TGG + chr1_3:29022416-29022435 None:intergenic 45.0%
!! CAGGATATGGGTTGGGTTTA+TGG + chr1_3:29022484-29022503 None:intergenic 45.0%
TCCGACAAAGGAACAAGAGA+CGG - chr1_3:29022864-29022883 Msa0113250:intron 45.0%
AACAAGAGACGGGAAAACAC+CGG - chr1_3:29022875-29022894 Msa0113250:intron 45.0%
AACTGACCGAAAAACTCCAC+CGG + chr1_3:29022897-29022916 None:intergenic 45.0%
GGAACTATTGGGTTTGTGCA+TGG - chr1_3:29023033-29023052 Msa0113250:intron 45.0%
TTTATCACCAAGGCAAGGAC+TGG - chr1_3:29023270-29023289 Msa0113250:five_prime_UTR 45.0%
GTGGAGGATAAGAATTCGGG+AGG - chr1_3:29022147-29022166 Msa0113250:intron 50.0%
GATGGTGCTCATCGATGAGA+AGG - chr1_3:29022210-29022229 Msa0113250:intron 50.0%
AGAGAGTCGACAAGAGCAAG+AGG - chr1_3:29022822-29022841 Msa0113250:intron 50.0%
CCCGTCTCTTGTTCCTTTGT+CGG + chr1_3:29022868-29022887 None:intergenic 50.0%
CCGACAAAGGAACAAGAGAC+GGG - chr1_3:29022865-29022884 Msa0113250:intron 50.0%
TAAGGATCCAGTCCTTGCCT+TGG + chr1_3:29023280-29023299 None:intergenic 50.0%
GTGGAGGATAAGAATTCGGG+AGG - chr1_3:29022147-29022166 Msa0113250:intron 50.0%
GATGGTGCTCATCGATGAGA+AGG - chr1_3:29022210-29022229 Msa0113250:intron 50.0%
AGAGAGTCGACAAGAGCAAG+AGG - chr1_3:29022822-29022841 Msa0113250:intron 50.0%
CCCGTCTCTTGTTCCTTTGT+CGG + chr1_3:29022868-29022887 None:intergenic 50.0%
CCGACAAAGGAACAAGAGAC+GGG - chr1_3:29022865-29022884 Msa0113250:intron 50.0%
TAAGGATCCAGTCCTTGCCT+TGG + chr1_3:29023280-29023299 None:intergenic 50.0%
!! GGAACTGCCACAAGGTATCG+AGG - chr1_3:29021820-29021839 Msa0113250:intron 55.0%
AGACAGAGCTGCTGACACGA+AGG + chr1_3:29021973-29021992 None:intergenic 55.0%
TCGTTCCTGGGAAAGATGCG+TGG - chr1_3:29022128-29022147 Msa0113250:intron 55.0%
TTCCTGGGAAAGATGCGTGG+AGG - chr1_3:29022131-29022150 Msa0113250:intron 55.0%
ATCCTCCACGCATCTTTCCC+AGG + chr1_3:29022136-29022155 None:intergenic 55.0%
AAGAGACGGGAAAACACCGG+TGG - chr1_3:29022878-29022897 Msa0113250:intron 55.0%
!! GGAACTGCCACAAGGTATCG+AGG - chr1_3:29021820-29021839 Msa0113250:intron 55.0%
AGACAGAGCTGCTGACACGA+AGG + chr1_3:29021973-29021992 None:intergenic 55.0%
TCGTTCCTGGGAAAGATGCG+TGG - chr1_3:29022128-29022147 Msa0113250:intron 55.0%
TTCCTGGGAAAGATGCGTGG+AGG - chr1_3:29022131-29022150 Msa0113250:intron 55.0%
ATCCTCCACGCATCTTTCCC+AGG + chr1_3:29022136-29022155 None:intergenic 55.0%
AAGAGACGGGAAAACACCGG+TGG - chr1_3:29022878-29022897 Msa0113250:intron 55.0%
CCAGAATCGGAGCCACACAG+TGG + chr1_3:29021716-29021735 None:intergenic 60.0%
CCACTGTGTGGCTCCGATTC+TGG - chr1_3:29021713-29021732 Msa0113250:CDS 60.0%
GTGGCTCCGATTCTGGTGCA+TGG - chr1_3:29021720-29021739 Msa0113250:intron 60.0%
CCAGAATCGGAGCCACACAG+TGG + chr1_3:29021716-29021735 None:intergenic 60.0%
CCACTGTGTGGCTCCGATTC+TGG - chr1_3:29021713-29021732 Msa0113250:CDS 60.0%
GTGGCTCCGATTCTGGTGCA+TGG - chr1_3:29021720-29021739 Msa0113250:intron 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 29021562 29023838 29021562 ID=Msa0113250;Name=Msa0113250
chr1_3 mRNA 29021562 29023838 29021562 ID=Msa0113250-mRNA-1;Parent=Msa0113250;Name=Msa0113250-mRNA-1;_AED=0.35;_eAED=0.41;_QI=662|0|0.33|1|0|0|3|0|97
chr1_3 exon 29021562 29021736 29021562 ID=Msa0113250-mRNA-1:exon:9632;Parent=Msa0113250-mRNA-1
chr1_3 exon 29022504 29022611 29022504 ID=Msa0113250-mRNA-1:exon:9631;Parent=Msa0113250-mRNA-1
chr1_3 exon 29023169 29023838 29023169 ID=Msa0113250-mRNA-1:exon:9630;Parent=Msa0113250-mRNA-1
chr1_3 five_prime_UTR 29023177 29023838 29023177 ID=Msa0113250-mRNA-1:five_prime_utr;Parent=Msa0113250-mRNA-1
chr1_3 CDS 29023169 29023176 29023169 ID=Msa0113250-mRNA-1:cds;Parent=Msa0113250-mRNA-1
chr1_3 CDS 29022504 29022611 29022504 ID=Msa0113250-mRNA-1:cds;Parent=Msa0113250-mRNA-1
chr1_3 CDS 29021562 29021736 29021562 ID=Msa0113250-mRNA-1:cds;Parent=Msa0113250-mRNA-1
Gene Sequence

>Msa0113250

ATGAGAAGACGGAAAACTCATTTGACGAAGGACGCGAAAAAAGAGAGTCGACAAGAGCAAGAGGACGCTAGAAAAGAGTACAATCCGACAAAGGAACAAGAGACGGGAAAACACCGGAATCCTACATGTTCAAATTGTGACAATCCAACTATTATTAGTGAGATTTCACTAGAGCAACACCGTCTTCAAATTGAAAATGCTAGGTTGAAGGATAAGTTAGACAGAGTTTATGCACTTTCTGTCACACACTTATTTGATGAGATTGAGATGGTGAAGGCAAGAAACAGAAGG
Protein sequence

>Msa0113250

MRRRKTHLTKDAKKESRQEQEDARKEYNPTKEQETGKHRNPTCSNCDNPTIISEISLEQHRLQIENARLKDKLDRVYALSVTHLFDEIEMVKARNRR