AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0114720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0114720 MtrunA17_Chr8g0335401 94.444 108 6 0 1 108 1 108 1.15e-73 216
Msa0114720 MtrunA17_Chr4g0064221 44.444 81 44 1 19 99 17 96 1.02e-15 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 58 sgRNAs with CRISPR-Local

Find 294 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTGACAGGCCCTGCTACC+TGG 0.202378 1_3:-32865356 Msa0114720:CDS
GGCTTCTGCAGGGTCTGTTT+TGG 0.233349 1_3:-32865421 Msa0114720:CDS
CTTTACCTCCCTTCTCTCTT+TGG 0.258123 1_3:+32867291 None:intergenic
TGGTGGGGCAGCATTGCTGC+TGG 0.286545 1_3:-32865384 Msa0114720:CDS
GTGACTGTGTGGTGGATTGT+AGG 0.313524 1_3:+32869398 None:intergenic
ATTCAAATTGTTTGGATGTA+AGG 0.324719 1_3:-32867404 Msa0114720:CDS
TATTACCCAGTAAGCATTGA+TGG 0.325248 1_3:-32867619 Msa0114720:intron
GCATGTTGATTCAAATTGTT+TGG 0.325712 1_3:-32867412 Msa0114720:CDS
TTTGGATGTAAGGGAGCTTA+TGG 0.327796 1_3:-32867394 Msa0114720:CDS
AAGTTCCGATTGCCATAAAA+AGG 0.336981 1_3:+32867548 None:intergenic
CTCCACCCGTTGGGTCTCAT+TGG 0.346371 1_3:+32867593 None:intergenic
AATCGGAACTTACTAATATT+AGG 0.357086 1_3:-32867536 Msa0114720:CDS
TCAAGTCTTTCCTGAACAAT+TGG 0.378778 1_3:-32867361 Msa0114720:CDS
AGTTTGTGTTCGGCTTCTGC+AGG 0.387437 1_3:-32865432 Msa0114720:CDS
GCTGCTGGGTTGCCTGTGAC+AGG 0.390418 1_3:-32865369 Msa0114720:CDS
CTACATGGGCTTTGCTAACA+TGG 0.391312 1_3:+32869278 None:intergenic
CCTAACTGCAACTCCAAGTC+GGG 0.397876 1_3:+32867501 None:intergenic
TTATTGAGAGTTTAGAGTTA+CGG 0.414829 1_3:+32869229 None:intergenic
CTCATATTTCTCCACCCGTT+GGG 0.420856 1_3:+32867584 None:intergenic
TATATATCTTCTTTGCTACA+TGG 0.423467 1_3:+32869263 None:intergenic
AACATACACGCCCAGGTAGC+AGG 0.424309 1_3:+32865345 None:intergenic
GGTGGGGCAGCATTGCTGCT+GGG 0.441820 1_3:-32865383 Msa0114720:CDS
CCCGACTTGGAGTTGCAGTT+AGG 0.446378 1_3:-32867501 Msa0114720:CDS
TGTGACAGGCCCTGCTACCT+GGG 0.449802 1_3:-32865355 Msa0114720:CDS
TTCAAATTGTTTGGATGTAA+GGG 0.461026 1_3:-32867403 Msa0114720:CDS
CATGAAGATGATGGACTCTA+TGG 0.464428 1_3:-32867475 Msa0114720:CDS
ATATATCTTCTTTGCTACAT+GGG 0.479371 1_3:+32869264 None:intergenic
AATTTCATCGGCAGCGGCAG+CGG 0.491118 1_3:-32869207 Msa0114720:five_prime_UTR
GTGGTTCATAATATGGGAAG+TGG 0.493088 1_3:-32869344 Msa0114720:five_prime_UTR
TAGCACAAGATGCACTAAAC+CGG 0.496645 1_3:+32865162 None:intergenic
GAAGCAAGTGTGCCCCGACT+TGG 0.497280 1_3:-32867514 Msa0114720:CDS
CTCTCTTGTAACAACAACTA+AGG 0.499557 1_3:-32869182 Msa0114720:intron
TGTTGGTGGTAGTTTGTGTT+CGG 0.499710 1_3:-32865442 Msa0114720:intron
CAGGTAGCAGGGCCTGTCAC+AGG 0.500562 1_3:+32865357 None:intergenic
GCTCATATTTCTCCACCCGT+TGG 0.508922 1_3:+32867583 None:intergenic
TTCCTGTTGTTTAGGTGATG+TGG 0.523588 1_3:-32865214 Msa0114720:intron
TTTGTGTTGTGACTGTGTGG+TGG 0.540669 1_3:+32869390 None:intergenic
AAACTCTCAATAAATTTCAT+CGG 0.546048 1_3:-32869219 Msa0114720:five_prime_UTR
CAAACATTACCAAAGAGAGA+AGG 0.550086 1_3:-32867300 Msa0114720:CDS
GTTTGTGTTCGGCTTCTGCA+GGG 0.554174 1_3:-32865431 Msa0114720:CDS
TTGAGAGTTTAGAGTTACGG+AGG 0.559735 1_3:+32869232 None:intergenic
GACTTGGAGTTGCAGTTAGG+TGG 0.560422 1_3:-32867498 Msa0114720:CDS
ATGCACTAAACCGGCAAAGG+AGG 0.561861 1_3:+32865171 None:intergenic
GGAAACCAATGAGACCCAAC+GGG 0.561936 1_3:-32867598 Msa0114720:CDS
GTTAGGTGGCATGAAGATGA+TGG 0.565745 1_3:-32867484 Msa0114720:CDS
TGGTTCATAATATGGGAAGT+GGG 0.570385 1_3:-32869343 Msa0114720:five_prime_UTR
TCAATAAATTTCATCGGCAG+CGG 0.571726 1_3:-32869213 Msa0114720:five_prime_UTR
AAACATTACCAAAGAGAGAA+GGG 0.572808 1_3:-32867299 Msa0114720:CDS
ACATACACGCCCAGGTAGCA+GGG 0.584167 1_3:+32865346 None:intergenic
ACCTAACTGCAACTCCAAGT+CGG 0.590338 1_3:+32867500 None:intergenic
AAGATGCACTAAACCGGCAA+AGG 0.609115 1_3:+32865168 None:intergenic
GAATTTGTGTTGTGACTGTG+TGG 0.616948 1_3:+32869387 None:intergenic
AACCAATGAGACCCAACGGG+TGG 0.636410 1_3:-32867595 Msa0114720:CDS
TGGAAACCAATGAGACCCAA+CGG 0.645617 1_3:-32867599 Msa0114720:CDS
TAACAGCTTCTAAACCACGT+TGG 0.692441 1_3:+32867325 None:intergenic
AGGAAAGACTTGATAAACTG+CGG 0.698525 1_3:+32867371 None:intergenic
CTAACTGCAACTCCAAGTCG+GGG 0.703026 1_3:+32867502 None:intergenic
CATTACCAAAGAGAGAAGGG+AGG 0.724224 1_3:-32867296 Msa0114720:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGATAATTTTTAAACAAAA+CGG - chr1_3:32865270-32865289 Msa0114720:intron 10.0%
!! ATATATATATATATATATCG+AGG + chr1_3:32865690-32865709 None:intergenic 10.0%
!! TATTCATAATATGAAATTAA+AGG - chr1_3:32866682-32866701 Msa0114720:intron 10.0%
!! ATTCATAATATGAAATTAAA+GGG - chr1_3:32866683-32866702 Msa0114720:intron 10.0%
!!! TATATTAAATTATGTCATTT+AGG + chr1_3:32867322-32867341 None:intergenic 10.0%
!!! TTTAAGTATTTTGTATGATT+TGG - chr1_3:32865435-32865454 Msa0114720:intron 15.0%
!!! TTAAGTATTTTGTATGATTT+GGG - chr1_3:32865436-32865455 Msa0114720:intron 15.0%
!! TATTTGTGTTCAAATTTATT+CGG - chr1_3:32865517-32865536 Msa0114720:intron 15.0%
!! AAAAAAATAATAAATGCATC+TGG - chr1_3:32866517-32866536 Msa0114720:intron 15.0%
!! AGGACAAAAATAAAATTAAA+TGG - chr1_3:32866565-32866584 Msa0114720:intron 15.0%
!! GGACAAAAATAAAATTAAAT+GGG - chr1_3:32866566-32866585 Msa0114720:intron 15.0%
!!! ATTTTTTTCATAAAGTACAT+TGG - chr1_3:32867606-32867625 Msa0114720:exon 15.0%
!!! TTTTATGTGGTTCATAATAT+GGG - chr1_3:32865230-32865249 Msa0114720:intron 20.0%
!! AAACTCTCAATAAATTTCAT+CGG - chr1_3:32865361-32865380 Msa0114720:CDS 20.0%
!! TTATATATGAATTGGTTGAT+TGG - chr1_3:32865475-32865494 Msa0114720:intron 20.0%
!! ATTTGTGTTCAAATTTATTC+GGG - chr1_3:32865518-32865537 Msa0114720:intron 20.0%
!!! CATTTTCCTAAATTTATTAG+TGG + chr1_3:32865985-32866004 None:intergenic 20.0%
!!! AGATGGAGTATTAAATTTAT+GGG + chr1_3:32866173-32866192 None:intergenic 20.0%
!! AGAAATGTGAAAAAGAAAAT+GGG + chr1_3:32866213-32866232 None:intergenic 20.0%
!! AAGAAATGTGAAAAAGAAAA+TGG + chr1_3:32866214-32866233 None:intergenic 20.0%
!! TTTAATACACATACAAACAA+AGG + chr1_3:32866296-32866315 None:intergenic 20.0%
!!! TACTCAAATTTTCCTTTTTA+TGG - chr1_3:32867020-32867039 Msa0114720:intron 20.0%
!!! TAATACATAAAAGCATACAA+TGG + chr1_3:32867413-32867432 None:intergenic 20.0%
!! ATAAGAGAATAAGTGTTAAA+AGG + chr1_3:32868295-32868314 None:intergenic 20.0%
!! TTTAACACTTATTCTCTTAT+TGG - chr1_3:32868295-32868314 Msa0114720:intron 20.0%
!! TTAACACTTATTCTCTTATT+GGG - chr1_3:32868296-32868315 Msa0114720:intron 20.0%
!!! TTATTAGTGATTTTGTGAAA+AGG - chr1_3:32868799-32868818 Msa0114720:intron 20.0%
!!! TTTTGTTGGTTCTTTTATTT+AGG - chr1_3:32869325-32869344 Msa0114720:five_prime_UTR 20.0%
!! ACAACATAAATCGTTTTATG+TGG - chr1_3:32865217-32865236 Msa0114720:intron 25.0%
!! GTTTTATGTGGTTCATAATA+TGG - chr1_3:32865229-32865248 Msa0114720:intron 25.0%
! ATATATCTTCTTTGCTACAT+GGG + chr1_3:32865319-32865338 None:intergenic 25.0%
! TATATATCTTCTTTGCTACA+TGG + chr1_3:32865320-32865339 None:intergenic 25.0%
!! TTATTGAGAGTTTAGAGTTA+CGG + chr1_3:32865354-32865373 None:intergenic 25.0%
!! ATTGCAAAAACTACTACTTT+TGG + chr1_3:32866041-32866060 None:intergenic 25.0%
!! AAAAGTAGTAGTTTTTGCAA+TGG - chr1_3:32866040-32866059 Msa0114720:intron 25.0%
!! CAGATGGAGTATTAAATTTA+TGG + chr1_3:32866174-32866193 None:intergenic 25.0%
!! TTTTTGCTTATAAATGTGAC+CGG + chr1_3:32866461-32866480 None:intergenic 25.0%
! CATTGAATGACTAATGTATA+TGG - chr1_3:32866387-32866406 Msa0114720:intron 25.0%
! ATGTATATGGTCACATTTAT+AGG - chr1_3:32866400-32866419 Msa0114720:intron 25.0%
!! TTTTTGCTTATAAATGTGAC+CGG - chr1_3:32866461-32866480 Msa0114720:intron 25.0%
!!! GAAATTTTGAGTAAAACTTG+AGG + chr1_3:32866656-32866675 None:intergenic 25.0%
! AGGTATATAATTGGTATTTG+TGG + chr1_3:32866725-32866744 None:intergenic 25.0%
!! TTGTACTAGAGGTATATAAT+TGG + chr1_3:32866734-32866753 None:intergenic 25.0%
!! GTTGATGAATGAAAAACAAA+TGG + chr1_3:32866908-32866927 None:intergenic 25.0%
!! ATTTTCCTTTTTATGGCAAT+CGG - chr1_3:32867027-32867046 Msa0114720:intron 25.0%
! AATCGGAACTTACTAATATT+AGG - chr1_3:32867044-32867063 Msa0114720:intron 25.0%
! ATTCAAATTGTTTGGATGTA+AGG - chr1_3:32867176-32867195 Msa0114720:intron 25.0%
! TTCAAATTGTTTGGATGTAA+GGG - chr1_3:32867177-32867196 Msa0114720:intron 25.0%
! AACTATAAGAATGTGTCTAA+AGG - chr1_3:32867458-32867477 Msa0114720:CDS 25.0%
! TGCTAACTACAAAAAATCTA+GGG + chr1_3:32867521-32867540 None:intergenic 25.0%
! TTGCTAACTACAAAAAATCT+AGG + chr1_3:32867522-32867541 None:intergenic 25.0%
! ATTGGAAACATGCTAATATA+AGG + chr1_3:32867766-32867785 None:intergenic 25.0%
!! ATGTTTCCAATAGTTTTCTA+TGG - chr1_3:32867775-32867794 Msa0114720:intron 25.0%
! AGAAAACCATAGAAAACTAT+TGG + chr1_3:32867784-32867803 None:intergenic 25.0%
!! ATTGAGACTTTTTATATTGC+TGG - chr1_3:32867982-32868001 Msa0114720:intron 25.0%
!!! TGAGACTTATTTTATGTTAC+TGG - chr1_3:32868011-32868030 Msa0114720:intron 25.0%
!! TGTTTTATGTTGTTGTATCA+TGG - chr1_3:32868049-32868068 Msa0114720:intron 25.0%
! ACAGTTATTAAAAAGAAACG+CGG + chr1_3:32868184-32868203 None:intergenic 25.0%
!!! CTTTTTATTGGCTTAAATCA+TGG + chr1_3:32868381-32868400 None:intergenic 25.0%
! TTAATTATTTCACGCATTTG+TGG - chr1_3:32868557-32868576 Msa0114720:intron 25.0%
! TAGATGATAATATTCCAGTT+TGG - chr1_3:32868590-32868609 Msa0114720:intron 25.0%
! AAAAAAAAGCAATTCCAAAC+TGG + chr1_3:32868607-32868626 None:intergenic 25.0%
! TTGTTAATAATTGTTCCATG+TGG - chr1_3:32868628-32868647 Msa0114720:intron 25.0%
! TTCAATTCGATCAGTTTAAT+AGG + chr1_3:32868695-32868714 None:intergenic 25.0%
! TTGATACTATTCTGCAAAAA+TGG - chr1_3:32868842-32868861 Msa0114720:intron 25.0%
! CTTTGCTAACATGGATTTTT+GGG + chr1_3:32865296-32865315 None:intergenic 30.0%
TTGTATATTAAGATTCGCTC+CGG - chr1_3:32865591-32865610 Msa0114720:intron 30.0%
ATGCATCTGGTAATTAGAAA+AGG - chr1_3:32866530-32866549 Msa0114720:intron 30.0%
TGCATCTGGTAATTAGAAAA+GGG - chr1_3:32866531-32866550 Msa0114720:intron 30.0%
AGAAAAGGGGTTTACATTTA+AGG - chr1_3:32866545-32866564 Msa0114720:intron 30.0%
TAAATGGGTCCTTAGATATA+AGG - chr1_3:32866581-32866600 Msa0114720:intron 30.0%
!! GCATGTTGATTCAAATTGTT+TGG - chr1_3:32867168-32867187 Msa0114720:intron 30.0%
AAACATTACCAAAGAGAGAA+GGG - chr1_3:32867281-32867300 Msa0114720:intron 30.0%
!! TATTAGTTTAGCTTGTGTGA+TGG - chr1_3:32867428-32867447 Msa0114720:CDS 30.0%
GGGTCTAATAGTTTGATAAA+AGG + chr1_3:32867501-32867520 None:intergenic 30.0%
TGTATATATGCTAGTTGACT+TGG - chr1_3:32867915-32867934 Msa0114720:intron 30.0%
GGGTTATTGGATAAATTATG+TGG - chr1_3:32867947-32867966 Msa0114720:intron 30.0%
!!! CTCTAACACTTGCTTTTTAT+TGG + chr1_3:32868393-32868412 None:intergenic 30.0%
ATAAAAAGCAAGTGTTAGAG+AGG - chr1_3:32868393-32868412 Msa0114720:intron 30.0%
TAAAAAGCAAGTGTTAGAGA+GGG - chr1_3:32868394-32868413 Msa0114720:intron 30.0%
ATAAATCAATTGTCACCACA+TGG + chr1_3:32868646-32868665 None:intergenic 30.0%
TTAATAGGAGAAATTTGAGC+TGG + chr1_3:32868680-32868699 None:intergenic 30.0%
TAAACTGATCGAATTGAAGA+TGG - chr1_3:32868697-32868716 Msa0114720:intron 30.0%
AAACTGATCGAATTGAAGAT+GGG - chr1_3:32868698-32868717 Msa0114720:intron 30.0%
ATACTATTCTGCAAAAATGG+TGG - chr1_3:32868845-32868864 Msa0114720:intron 30.0%
!! TTGTTGTTGTCTGGTTATTT+TGG - chr1_3:32869101-32869120 Msa0114720:intron 30.0%
!! TGTTGTTGTCTGGTTATTTT+GGG - chr1_3:32869102-32869121 Msa0114720:intron 30.0%
!! GTATTTTGTTCCTGTTGTTT+AGG - chr1_3:32869358-32869377 Msa0114720:five_prime_UTR 30.0%
TGGTTCATAATATGGGAAGT+GGG - chr1_3:32865237-32865256 Msa0114720:intron 35.0%
! GCTTTGCTAACATGGATTTT+TGG + chr1_3:32865297-32865316 None:intergenic 35.0%
TCAATAAATTTCATCGGCAG+CGG - chr1_3:32865367-32865386 Msa0114720:CDS 35.0%
CTCTCTTGTAACAACAACTA+AGG - chr1_3:32865398-32865417 Msa0114720:CDS 35.0%
ATTTGCATTTCTCTATCCAC+CGG + chr1_3:32865613-32865632 None:intergenic 35.0%
GTGGATAGAGAAATGCAAAT+TGG - chr1_3:32865613-32865632 Msa0114720:intron 35.0%
!! AATTGGTTCGCACTAATTTG+CGG - chr1_3:32865630-32865649 Msa0114720:intron 35.0%
! TAATTTGCGGTCTAATTCGT+TGG - chr1_3:32865643-32865662 Msa0114720:intron 35.0%
GTTGTCGATGACACTAAAAT+TGG - chr1_3:32865911-32865930 Msa0114720:intron 35.0%
GTGTGGCCACTAATAAATTT+AGG - chr1_3:32865976-32865995 Msa0114720:intron 35.0%
ATGCTTGAACAATACCTGAA+GGG + chr1_3:32866018-32866037 None:intergenic 35.0%
!! ATTCATTTTTGTGCGTTCCT+TGG - chr1_3:32866116-32866135 Msa0114720:intron 35.0%
TTTGCTAACAAGTGCCTTAA+AGG + chr1_3:32866148-32866167 None:intergenic 35.0%
AAAATGGGTGCCTACATTAA+GGG + chr1_3:32866198-32866217 None:intergenic 35.0%
GCATCTGGTAATTAGAAAAG+GGG - chr1_3:32866532-32866551 Msa0114720:intron 35.0%
! AACTCGATAACTTGTACTAG+AGG + chr1_3:32866745-32866764 None:intergenic 35.0%
!! TATTACCCAGTAAGCATTGA+TGG - chr1_3:32866961-32866980 Msa0114720:intron 35.0%
AAGTTCCGATTGCCATAAAA+AGG + chr1_3:32867035-32867054 None:intergenic 35.0%
AGGAAAGACTTGATAAACTG+CGG + chr1_3:32867212-32867231 None:intergenic 35.0%
TCAAGTCTTTCCTGAACAAT+TGG - chr1_3:32867219-32867238 Msa0114720:intron 35.0%
CAAACATTACCAAAGAGAGA+AGG - chr1_3:32867280-32867299 Msa0114720:intron 35.0%
! GTGATAGTGTGATAATAGCA+AGG + chr1_3:32867389-32867408 None:intergenic 35.0%
TTATCCATAAGCTTGTGACA+AGG + chr1_3:32867687-32867706 None:intergenic 35.0%
ACTTCAATTGTGATATGCAG+AGG - chr1_3:32867831-32867850 Msa0114720:intron 35.0%
ATTGTGATATGCAGAGGTAA+TGG - chr1_3:32867837-32867856 Msa0114720:intron 35.0%
TTGTGATATGCAGAGGTAAT+GGG - chr1_3:32867838-32867857 Msa0114720:intron 35.0%
!! TAGTTGACTTGGATTTGACT+TGG - chr1_3:32867926-32867945 Msa0114720:intron 35.0%
!! AGTTGACTTGGATTTGACTT+GGG - chr1_3:32867927-32867946 Msa0114720:intron 35.0%
!! TTGGATTTGACTTGGGTTAT+TGG - chr1_3:32867934-32867953 Msa0114720:intron 35.0%
! AGAGCTCACACTTTAGAAAT+GGG + chr1_3:32868357-32868376 None:intergenic 35.0%
TTGCTAGTATTACTCTTGAG+AGG - chr1_3:32868431-32868450 Msa0114720:intron 35.0%
!!! AGTTTTAATTCGCTATGCAG+TGG - chr1_3:32868471-32868490 Msa0114720:intron 35.0%
CTACTGGTATCAGTTTATAG+TGG + chr1_3:32868894-32868913 None:intergenic 35.0%
CACTATAAACTGATACCAGT+AGG - chr1_3:32868892-32868911 Msa0114720:intron 35.0%
!!! TGTGTTGGTTGTGTGTTTTT+GGG + chr1_3:32868938-32868957 None:intergenic 35.0%
! TTCCATCACCATTCTGTTTT+GGG + chr1_3:32869056-32869075 None:intergenic 35.0%
! ATTCCATCACCATTCTGTTT+TGG + chr1_3:32869057-32869076 None:intergenic 35.0%
!!! TGTATGTTTTTTCTGCTTGC+TGG - chr1_3:32869250-32869269 Msa0114720:five_prime_UTR 35.0%
TAAAAGAACCAACAAAACGC+AGG + chr1_3:32869322-32869341 None:intergenic 35.0%
GGAGGAAAAACAGTACAAAT+AGG + chr1_3:32869394-32869413 None:intergenic 35.0%
GAATTTGTGTTGTGACTGTG+TGG + chr1_3:32865196-32865215 None:intergenic 40.0%
GTGGTTCATAATATGGGAAG+TGG - chr1_3:32865236-32865255 Msa0114720:intron 40.0%
! TTGAGAGTTTAGAGTTACGG+AGG + chr1_3:32865351-32865370 None:intergenic 40.0%
!! TCGGGATTTGTTGTAATTGC+TGG - chr1_3:32865536-32865555 Msa0114720:intron 40.0%
!! GGATTTGTTGTAATTGCTGG+TGG - chr1_3:32865539-32865558 Msa0114720:intron 40.0%
TATATTAAGATTCGCTCCGG+TGG - chr1_3:32865594-32865613 Msa0114720:intron 40.0%
ACCACACGAGAAAAGACTAA+GGG + chr1_3:32865884-32865903 None:intergenic 40.0%
TACCACACGAGAAAAGACTA+AGG + chr1_3:32865885-32865904 None:intergenic 40.0%
! ACACTAAAATTGGCACTGAG+TGG - chr1_3:32865921-32865940 Msa0114720:intron 40.0%
! CACTAAAATTGGCACTGAGT+GGG - chr1_3:32865922-32865941 Msa0114720:intron 40.0%
GATGCTTGAACAATACCTGA+AGG + chr1_3:32866019-32866038 None:intergenic 40.0%
GAAAATGGGTGCCTACATTA+AGG + chr1_3:32866199-32866218 None:intergenic 40.0%
TGCTTATAAATGTGACCGGA+GGG + chr1_3:32866465-32866484 None:intergenic 40.0%
TTGCTTATAAATGTGACCGG+AGG + chr1_3:32866464-32866483 None:intergenic 40.0%
TTGCTTATAAATGTGACCGG+AGG - chr1_3:32866464-32866483 Msa0114720:intron 40.0%
TGCTTATAAATGTGACCGGA+GGG - chr1_3:32866465-32866484 Msa0114720:intron 40.0%
CCAATTAATACTACTCCCTC+CGG + chr1_3:32866483-32866502 None:intergenic 40.0%
CGGAGGGAGTAGTATTAATT+GGG - chr1_3:32866481-32866500 Msa0114720:intron 40.0%
GGAGGGAGTAGTATTAATTG+GGG - chr1_3:32866482-32866501 Msa0114720:intron 40.0%
GAGGGAGTAGTATTAATTGG+GGG - chr1_3:32866483-32866502 Msa0114720:intron 40.0%
TGGGTCCTTAGATATAAGGA+CGG - chr1_3:32866585-32866604 Msa0114720:intron 40.0%
TCCTTAGATATAAGGACGGA+GGG - chr1_3:32866589-32866608 Msa0114720:intron 40.0%
! GCAGCTATAACGGAAAAATC+AGG + chr1_3:32866827-32866846 None:intergenic 40.0%
! GATTTTTCCGTTATAGCTGC+TGG - chr1_3:32866827-32866846 Msa0114720:intron 40.0%
ACCACCTCTACTGTCAAAAA+AGG + chr1_3:32866858-32866877 None:intergenic 40.0%
GACAGTAGAGGTGGTAATTA+TGG - chr1_3:32866863-32866882 Msa0114720:intron 40.0%
GAGGTGGTAATTATGGTGTA+AGG - chr1_3:32866870-32866889 Msa0114720:intron 40.0%
GGTTTCCATCAATGCTTACT+GGG + chr1_3:32866969-32866988 None:intergenic 40.0%
TGGTTTCCATCAATGCTTAC+TGG + chr1_3:32866970-32866989 None:intergenic 40.0%
!! CATGAAGATGATGGACTCTA+TGG - chr1_3:32867105-32867124 Msa0114720:intron 40.0%
TTTGGATGTAAGGGAGCTTA+TGG - chr1_3:32867186-32867205 Msa0114720:intron 40.0%
!!! GCATTTTTGGCCAATTGTTC+AGG + chr1_3:32867232-32867251 None:intergenic 40.0%
TAACAGCTTCTAAACCACGT+TGG + chr1_3:32867258-32867277 None:intergenic 40.0%
TCTACCTTGTCACAAGCTTA+TGG - chr1_3:32867680-32867699 Msa0114720:intron 40.0%
ACTTACGCTAAAGATGCTCT+TGG + chr1_3:32868225-32868244 None:intergenic 40.0%
! AGAGCATCTTTAGCGTAAGT+TGG - chr1_3:32868225-32868244 Msa0114720:intron 40.0%
!! AGCGTAAGTTGGTTATTGAG+AGG - chr1_3:32868236-32868255 Msa0114720:intron 40.0%
AGTTGGTTATTGAGAGGTTG+AGG - chr1_3:32868242-32868261 Msa0114720:intron 40.0%
!! AATGTTGAAAAGAGTGTCCC+TGG + chr1_3:32868272-32868291 None:intergenic 40.0%
GTATGACCAACCACTTTGAA+TGG - chr1_3:32868329-32868348 Msa0114720:intron 40.0%
! GAAATGGGTTCCATTCAAAG+TGG + chr1_3:32868342-32868361 None:intergenic 40.0%
! CAGAGCTCACACTTTAGAAA+TGG + chr1_3:32868358-32868377 None:intergenic 40.0%
TGATGTGTGTTTGACGGTAA+AGG - chr1_3:32868721-32868740 Msa0114720:intron 40.0%
GATGTGTGTTTGACGGTAAA+GGG - chr1_3:32868722-32868741 Msa0114720:intron 40.0%
GTATCAGTTTATAGTGGTGC+TGG + chr1_3:32868888-32868907 None:intergenic 40.0%
!!! GTGTGTTGGTTGTGTGTTTT+TGG + chr1_3:32868939-32868958 None:intergenic 40.0%
!!! GACGTGTATGTTTTTTCTGC+TGG + chr1_3:32868970-32868989 None:intergenic 40.0%
CAGAAAAAACATACACGTCC+AGG - chr1_3:32868971-32868990 Msa0114720:intron 40.0%
!! TTTTGGGAGCTGTTGTGTTT+CGG + chr1_3:32869176-32869195 None:intergenic 40.0%
!! AGAATGGTGATGGAATCCTT+TGG - chr1_3:32869061-32869080 Msa0114720:intron 40.0%
ACAACAACAACTATCTCGCA+AGG + chr1_3:32869091-32869110 None:intergenic 40.0%
!! TGTTGGTGGTAGTTTGTGTT+CGG - chr1_3:32869138-32869157 Msa0114720:intron 40.0%
!! TTTTGGGAGCTGTTGTGTTT+TGG - chr1_3:32869176-32869195 Msa0114720:intron 40.0%
! TTCCTGTTGTTTAGGTGATG+TGG - chr1_3:32869366-32869385 Msa0114720:five_prime_UTR 40.0%
!! TACTGTTTTTCCTCCTTTGC+CGG - chr1_3:32869399-32869418 Msa0114720:five_prime_UTR 40.0%
TTTGTGTTGTGACTGTGTGG+TGG + chr1_3:32865193-32865212 None:intergenic 45.0%
CTACATGGGCTTTGCTAACA+TGG + chr1_3:32865305-32865324 None:intergenic 45.0%
ACGCGAATTGGTAGAGATGA+AGG - chr1_3:32865566-32865585 Msa0114720:intron 45.0%
TATATATCGAGGTGCGCATC+AGG + chr1_3:32865679-32865698 None:intergenic 45.0%
!! ACCCTTAGTCTTTTCTCGTG+TGG - chr1_3:32865880-32865899 Msa0114720:intron 45.0%
AATGCTAACAAGTGCCCTTC+AGG - chr1_3:32866001-32866020 Msa0114720:intron 45.0%
TTCCTTGGACGATGCCTTTA+AGG - chr1_3:32866131-32866150 Msa0114720:intron 45.0%
CTCCATCTGTCCCTTAATGT+AGG - chr1_3:32866185-32866204 Msa0114720:intron 45.0%
TGCCTACATTAAGGGACAGA+TGG + chr1_3:32866190-32866209 None:intergenic 45.0%
TTACAAGATAGTGCTCCCTC+CGG - chr1_3:32866330-32866349 Msa0114720:intron 45.0%
CCGGAGGGAGTAGTATTAAT+TGG - chr1_3:32866480-32866499 Msa0114720:intron 45.0%
GTCCTTAGATATAAGGACGG+AGG - chr1_3:32866588-32866607 Msa0114720:intron 45.0%
TCCCTCCGTCCTTATATCTA+AGG + chr1_3:32866593-32866612 None:intergenic 45.0%
!!! GCCTTTTTTGACAGTAGAGG+TGG - chr1_3:32866854-32866873 Msa0114720:intron 45.0%
TGGAAACCAATGAGACCCAA+CGG - chr1_3:32866981-32867000 Msa0114720:intron 45.0%
CTCATATTTCTCCACCCGTT+GGG + chr1_3:32866999-32867018 None:intergenic 45.0%
ACCTAACTGCAACTCCAAGT+CGG + chr1_3:32867083-32867102 None:intergenic 45.0%
GTTAGGTGGCATGAAGATGA+TGG - chr1_3:32867096-32867115 Msa0114720:intron 45.0%
!! ACCACGTTGGTGAGCATTTT+TGG + chr1_3:32867245-32867264 None:intergenic 45.0%
CATTACCAAAGAGAGAAGGG+AGG - chr1_3:32867284-32867303 Msa0114720:intron 45.0%
CTTTACCTCCCTTCTCTCTT+TGG + chr1_3:32867292-32867311 None:intergenic 45.0%
ATAGCAAGGCGAAGTAGCAA+AGG + chr1_3:32867375-32867394 None:intergenic 45.0%
!! AGAGGTAATGGGTGATGAAG+AGG - chr1_3:32867849-32867868 Msa0114720:intron 45.0%
TGAAGAGGTCGTTGGAATCT+TGG - chr1_3:32867864-32867883 Msa0114720:intron 45.0%
GAAGAGGTCGTTGGAATCTT+GGG - chr1_3:32867865-32867884 Msa0114720:intron 45.0%
TGGGTTCCATTCAAAGTGGT+TGG + chr1_3:32868338-32868357 None:intergenic 45.0%
AGAAGTGGTGACATGGTAAG+TGG - chr1_3:32868494-32868513 Msa0114720:intron 45.0%
GAAGTGGTGACATGGTAAGT+GGG - chr1_3:32868495-32868514 Msa0114720:intron 45.0%
GATGGGTGATGTGTGTTTGA+CGG - chr1_3:32868715-32868734 Msa0114720:intron 45.0%
!!! GTTGGTTGTGTGTTTTTGGG+AGG + chr1_3:32868935-32868954 None:intergenic 45.0%
!!! TGTTTTTTCTGCTGGCAGCA+GGG + chr1_3:32868962-32868981 None:intergenic 45.0%
!!! ATGTTTTTTCTGCTGGCAGC+AGG + chr1_3:32868963-32868982 None:intergenic 45.0%
CAACAGCTCCCAAAACAGAA+TGG - chr1_3:32869045-32869064 Msa0114720:intron 45.0%
CTCCCAAAACAGAATGGTGA+TGG - chr1_3:32869051-32869070 Msa0114720:intron 45.0%
TATCTCGCAAGGTACACCAA+AGG + chr1_3:32869080-32869099 None:intergenic 45.0%
! GCGAGATAGTTGTTGTTGTC+TGG - chr1_3:32869092-32869111 Msa0114720:intron 45.0%
!!! TGGGTGCTGTTTTCTGTTGT+TGG - chr1_3:32869121-32869140 Msa0114720:intron 45.0%
!!! GTGCTGTTTTCTGTTGTTGG+TGG - chr1_3:32869124-32869143 Msa0114720:intron 45.0%
CAGAAAAAACATACACGCCC+AGG + chr1_3:32869245-32869264 None:intergenic 45.0%
CACCACATCACCTAAACAAC+AGG + chr1_3:32869371-32869390 None:intergenic 45.0%
!!! TTAATTTTTTATATATGAAT+TGG - chr1_3:32865467-32865486 Msa0114720:intron 5.0%
!! GTGACTGTGTGGTGGATTGT+AGG + chr1_3:32865185-32865204 None:intergenic 50.0%
! AAATTGGCACTGAGTGGGAG+CGG - chr1_3:32865927-32865946 Msa0114720:intron 50.0%
!! TGCCTTAAAGGCATCGTCCA+AGG + chr1_3:32866136-32866155 None:intergenic 50.0%
AAAATCAGGACCATCGCGAC+AGG + chr1_3:32866813-32866832 None:intergenic 50.0%
!!! GCGGCCTTTTTTGACAGTAG+AGG - chr1_3:32866851-32866870 Msa0114720:intron 50.0%
GGAAACCAATGAGACCCAAC+GGG - chr1_3:32866982-32867001 Msa0114720:intron 50.0%
GCTCATATTTCTCCACCCGT+TGG + chr1_3:32867000-32867019 None:intergenic 50.0%
CTAACTGCAACTCCAAGTCG+GGG + chr1_3:32867081-32867100 None:intergenic 50.0%
CCTAACTGCAACTCCAAGTC+GGG + chr1_3:32867082-32867101 None:intergenic 50.0%
GACTTGGAGTTGCAGTTAGG+TGG - chr1_3:32867082-32867101 Msa0114720:intron 50.0%
GCCAAAAATGCTCACCAACG+TGG - chr1_3:32867241-32867260 Msa0114720:intron 50.0%
ATGGGTGATGAAGAGGTCGT+TGG - chr1_3:32867856-32867875 Msa0114720:intron 50.0%
TTGAGAGGTTGAGGAATGCC+AGG - chr1_3:32868251-32868270 Msa0114720:intron 50.0%
TGAGAGGTTGAGGAATGCCA+GGG - chr1_3:32868252-32868271 Msa0114720:intron 50.0%
TTCGCTATGCAGTGGAGAAG+TGG - chr1_3:32868479-32868498 Msa0114720:intron 50.0%
GCAAAAATGGTGGCTACACC+AGG - chr1_3:32868855-32868874 Msa0114720:intron 50.0%
TGCAGGTGCTTAGATCCTAC+TGG + chr1_3:32868910-32868929 None:intergenic 50.0%
!!! TGTGTTTTTGGGAGGCTTGC+AGG + chr1_3:32868927-32868946 None:intergenic 50.0%
!!! GTTTTTTCTGCTGGCAGCAG+GGG + chr1_3:32868961-32868980 None:intergenic 50.0%
AGTTTGTGTTCGGCTTCTGC+AGG - chr1_3:32869148-32869167 Msa0114720:intron 50.0%
GTTTGTGTTCGGCTTCTGCA+GGG - chr1_3:32869149-32869168 Msa0114720:intron 50.0%
! GCTTCTGCAGGGTCTGTTTT+GGG - chr1_3:32869160-32869179 Msa0114720:intron 50.0%
!! TGGGAGCTGTTGTGTTTTGG+TGG - chr1_3:32869179-32869198 Msa0114720:intron 50.0%
!!! GGGAGCTGTTGTGTTTTGGT+GGG - chr1_3:32869180-32869199 Msa0114720:intron 50.0%
!!! GGAGCTGTTGTGTTTTGGTG+GGG - chr1_3:32869181-32869200 Msa0114720:intron 50.0%
GAAGTGAATCTGCCTACACC+AGG + chr1_3:32869283-32869302 None:intergenic 50.0%
! TGTTGCAGCCTGCGTTTTGT+TGG - chr1_3:32869311-32869330 Msa0114720:five_prime_UTR 50.0%
AATTTCATCGGCAGCGGCAG+CGG - chr1_3:32865373-32865392 Msa0114720:CDS 55.0%
! GCTGGTGGTGAAACGCGAAT+TGG - chr1_3:32865554-32865573 Msa0114720:intron 55.0%
! AATTGGCACTGAGTGGGAGC+GGG - chr1_3:32865928-32865947 Msa0114720:intron 55.0%
!!! GTGTGTAGCGGGCGCTATTT+TGG - chr1_3:32865951-32865970 Msa0114720:intron 55.0%
!!! TGTAGCGGGCGCTATTTTGG+TGG - chr1_3:32865954-32865973 Msa0114720:intron 55.0%
TTCCGTTATAGCTGCTGGCG+CGG - chr1_3:32866832-32866851 Msa0114720:intron 55.0%
AACCAATGAGACCCAACGGG+TGG - chr1_3:32866985-32867004 Msa0114720:intron 55.0%
!! CCCGACTTGGAGTTGCAGTT+AGG - chr1_3:32867079-32867098 Msa0114720:intron 55.0%
GCAGTGGAGAAGTGGTGACA+TGG - chr1_3:32868487-32868506 Msa0114720:intron 55.0%
AACATACACGTCCAGGCAGC+AGG - chr1_3:32868978-32868997 Msa0114720:intron 55.0%
GGAGCTGTTGTGTTTCGGTG+GGG + chr1_3:32869035-32869054 None:intergenic 55.0%
GGGAGCTGTTGTGTTTCGGT+GGG + chr1_3:32869036-32869055 None:intergenic 55.0%
TGGGAGCTGTTGTGTTTCGG+TGG + chr1_3:32869037-32869056 None:intergenic 55.0%
! GGCTTCTGCAGGGTCTGTTT+TGG - chr1_3:32869159-32869178 Msa0114720:intron 55.0%
ACATACACGCCCAGGTAGCA+GGG + chr1_3:32869237-32869256 None:intergenic 55.0%
AACATACACGCCCAGGTAGC+AGG + chr1_3:32869238-32869257 None:intergenic 55.0%
! CTGCTTGCTGGTTGATGTCC+TGG - chr1_3:32869262-32869281 Msa0114720:five_prime_UTR 55.0%
! GCTGGTTGATGTCCTGGTGT+AGG - chr1_3:32869268-32869287 Msa0114720:five_prime_UTR 55.0%
! ATTGGCACTGAGTGGGAGCG+GGG - chr1_3:32865929-32865948 Msa0114720:intron 60.0%
!!! CGGGCGCTATTTTGGTGGTG+TGG - chr1_3:32865959-32865978 Msa0114720:intron 60.0%
ACCATCGCGACAGGCAACGT+TGG + chr1_3:32866804-32866823 None:intergenic 60.0%
CTCCACCCGTTGGGTCTCAT+TGG + chr1_3:32866990-32867009 None:intergenic 60.0%
GAAGCAAGTGTGCCCCGACT+TGG - chr1_3:32867066-32867085 Msa0114720:intron 60.0%
!! AGTGGTGCTGGTTGCTGTCC+TGG + chr1_3:32868876-32868895 None:intergenic 60.0%
TGTGACAGGCCCTGCTACCT+GGG - chr1_3:32869225-32869244 Msa0114720:five_prime_UTR 60.0%
AGTGGGAGCGGGGTGTGTAG+CGG - chr1_3:32865939-32865958 Msa0114720:intron 65.0%
CGCGCGGCAGAAGGAGAAAG+CGG + chr1_3:32866781-32866800 None:intergenic 65.0%
GCCAACGTTGCCTGTCGCGA+TGG - chr1_3:32866800-32866819 Msa0114720:intron 65.0%
GGCCGCGCCAGCAGCTATAA+CGG + chr1_3:32866837-32866856 None:intergenic 65.0%
! TGCTGGCAGCAGGGGTGTGT+TGG + chr1_3:32868953-32868972 None:intergenic 65.0%
CTGTGACAGGTCCTGCTGCC+TGG + chr1_3:32868992-32869011 None:intergenic 65.0%
CAGGCAGCAGGACCTGTCAC+AGG - chr1_3:32868990-32869009 Msa0114720:intron 65.0%
GCTGCTGGGTTGCCTGTGAC+AGG + chr1_3:32869211-32869230 None:intergenic 65.0%
GGTGGGGCAGCATTGCTGCT+GGG + chr1_3:32869197-32869216 None:intergenic 65.0%
TGGTGGGGCAGCATTGCTGC+TGG - chr1_3:32869196-32869215 Msa0114720:five_prime_UTR 65.0%
GGTGGGGCAGCATTGCTGCT+GGG - chr1_3:32869197-32869216 Msa0114720:five_prime_UTR 65.0%
GCTGCTGGGTTGCCTGTGAC+AGG - chr1_3:32869211-32869230 Msa0114720:five_prime_UTR 65.0%
CAGGTAGCAGGGCCTGTCAC+AGG + chr1_3:32869226-32869245 None:intergenic 65.0%
CTGTGACAGGCCCTGCTACC+TGG - chr1_3:32869224-32869243 Msa0114720:five_prime_UTR 65.0%
GTGGGAGCGGGGTGTGTAGC+GGG - chr1_3:32865940-32865959 Msa0114720:intron 70.0%
CAACGTTGGCGCGCGGCAGA+AGG + chr1_3:32866790-32866809 None:intergenic 70.0%
CGACAGGCAACGTTGGCGCG+CGG + chr1_3:32866797-32866816 None:intergenic 70.0%
CGGTGGGGCAGCATTGCTGC+TGG + chr1_3:32869020-32869039 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 32865176 32869426 32865176 ID=Msa0114720;Name=Msa0114720
chr1_3 mRNA 32865176 32869426 32865176 ID=Msa0114720-mRNA-1;Parent=Msa0114720;Name=Msa0114720-mRNA-1;_AED=0.26;_eAED=0.26;_QI=253|0.33|0.5|0.5|0|0.25|4|0|157
chr1_3 exon 32865176 32865222 32865176 ID=Msa0114720-mRNA-1:exon:10389;Parent=Msa0114720-mRNA-1
chr1_3 exon 32865352 32865452 32865352 ID=Msa0114720-mRNA-1:exon:10388;Parent=Msa0114720-mRNA-1
chr1_3 exon 32867297 32867631 32867297 ID=Msa0114720-mRNA-1:exon:10387;Parent=Msa0114720-mRNA-1
chr1_3 exon 32869183 32869426 32869183 ID=Msa0114720-mRNA-1:exon:10386;Parent=Msa0114720-mRNA-1
chr1_3 five_prime_UTR 32869183 32869426 32869183 ID=Msa0114720-mRNA-1:five_prime_utr;Parent=Msa0114720-mRNA-1
chr1_3 five_prime_UTR 32867623 32867631 32867623 ID=Msa0114720-mRNA-1:five_prime_utr;Parent=Msa0114720-mRNA-1
chr1_3 CDS 32867297 32867622 32867297 ID=Msa0114720-mRNA-1:cds;Parent=Msa0114720-mRNA-1
chr1_3 CDS 32865352 32865452 32865352 ID=Msa0114720-mRNA-1:cds;Parent=Msa0114720-mRNA-1
chr1_3 CDS 32865176 32865222 32865176 ID=Msa0114720-mRNA-1:cds;Parent=Msa0114720-mRNA-1
Gene Sequence

>Msa0114720

ATGGAAACCAATGAGACCCAACGGGTGGAGAAATATGAGCTACTCAAATTTTCCTTTTTATGGCAATCGGAACTTACTAATATTAGGAAGCAAGTGTGCCCCGACTTGGAGTTGCAGTTAGGTGGCATGAAGATGATGGACTCTATGGTAAACTACTTCATGAAAACGCTTATTAAAGAAGCAACAAAGCATGTTGATTCAAATTGTTTGGATGTAAGGGAGCTTATGGCCGCAGTTTATCAAGTCTTTCCTGAACAATTGGCCAAAAATGCTCACCAACGTGGTTTAGAAGCTGTTAATCAAACATTACCAAAGAGAGAAGGGAGTTTGTGTTCGGCTTCTGCAGGGTCTGTTTTGGGAGCTGTTGTGTTTTGGTGGGGCAGCATTGCTGCTGGGTTGCCTGTGACAGGCCCTGCTACCTGGGCGTGTGATGTGGTGCCTATTTGTACTGTTTTTCCTCCTTTGCCGGTTTAG
Protein sequence

>Msa0114720

METNETQRVEKYELLKFSFLWQSELTNIRKQVCPDLELQLGGMKMMDSMVNYFMKTLIKEATKHVDSNCLDVRELMAAVYQVFPEQLAKNAHQRGLEAVNQTLPKREGSLCSASAGSVLGAVVFWWGSIAAGLPVTGPATWACDVVPICTVFPPLPV*