AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0116450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0116450 MtrunA17_Chr1g0193071 86.719 128 11 2 1 128 1 122 1.76e-70 207
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0116450 AT5G26749.1 40.136 147 69 2 1 128 1 147 5.47e-32 110
Msa0116450 AT5G26749.2 35.542 166 69 3 1 128 1 166 1.87e-27 99.8

Find 88 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACCTGGCATTCTTGTTAT+TGG 0.166998 1_3:+35458628 None:intergenic
TGCTGCTGCTGTTGTGTATT+GGG 0.205632 1_3:+35458652 None:intergenic
TACCGGCAGAGTGGCGTTTC+CGG 0.248184 1_3:+35458913 None:intergenic
AGGCAGGCATACAAGGAAAA+TGG 0.254879 1_3:+35459393 None:intergenic
CCTCAACTCTACTACAAATA+TGG 0.258437 1_3:+35459022 None:intergenic
AGAGTGGCGTTTCCGGTCTA+AGG 0.283984 1_3:+35458920 None:intergenic
TTCTTTCTACGCTCACATTA+AGG 0.284952 1_3:-35459245 Msa0116450:intron
TCATTATAGCCATAAAATAA+TGG 0.316834 1_3:+35458359 None:intergenic
TTGCTGCTGCTGTTGTGTAT+TGG 0.317673 1_3:+35458651 None:intergenic
GGAATAGAGTTCTGGTGTTC+GGG 0.348282 1_3:+35458846 None:intergenic
AGATTTGTGCACATTACCTT+TGG 0.358949 1_3:-35458398 Msa0116450:three_prime_UTR
ATAACAAGGTAACATTTAAC+AGG 0.361622 1_3:+35458257 None:intergenic
ATGTGAGCGTAGAAAGAAAT+TGG 0.367973 1_3:+35459250 None:intergenic
TGGAATAGAGTTCTGGTGTT+CGG 0.377509 1_3:+35458845 None:intergenic
AGTCCGACAACCTCTGTTCT+AGG 0.387209 1_3:-35459362 Msa0116450:five_prime_UTR
ATCTATCCCCAGTCGACAAA+TGG 0.397656 1_3:+35458417 None:intergenic
AGGCGTGGGTGTGGTGAGGA+GGG 0.402639 1_3:-35459002 Msa0116450:five_prime_UTR
GAGGCGTGGGTGTGGTGAGG+AGG 0.413445 1_3:-35459003 Msa0116450:five_prime_UTR
CAGGCAGAACGGCGGCGTCC+TGG 0.417159 1_3:+35459320 None:intergenic
ATGGTGGTGGAATAGAGTTC+TGG 0.430967 1_3:+35458838 None:intergenic
GGAATCGTACCAACGAGCTT+TGG 0.434321 1_3:+35458875 None:intergenic
ATAAAATAATGGTTGCATAC+AGG 0.435792 1_3:+35458370 None:intergenic
GATTTGTGCACATTACCTTT+GGG 0.444503 1_3:-35458397 Msa0116450:three_prime_UTR
CAACCTCCCATTTGTCGACT+GGG 0.457123 1_3:-35458424 Msa0116450:CDS
GTCACTGCAGCCTCCGCCTG+AGG 0.458331 1_3:-35458457 Msa0116450:CDS
TCACTGCAGCCTCCGCCTGA+GGG 0.463350 1_3:-35458456 Msa0116450:CDS
GTTCTGGTGTTCGGGTTTGA+AGG 0.464430 1_3:+35458854 None:intergenic
AACGGAGGCGTCGCATAGAA+TGG 0.465306 1_3:+35459291 None:intergenic
ACGCAGAAGGGTTGATTAGA+TGG 0.466779 1_3:+35458819 None:intergenic
GGCAGGCATACAAGGAAAAT+GGG 0.474158 1_3:+35459394 None:intergenic
GGCTTGTTTGTGTTGAATAC+CGG 0.496651 1_3:+35458896 None:intergenic
GGCGTGGGTGTGGTGAGGAG+GGG 0.501306 1_3:-35459001 Msa0116450:five_prime_UTR
TCTATCCCCAGTCGACAAAT+GGG 0.506048 1_3:+35458418 None:intergenic
TACTGGTTATTACAACTACT+AGG 0.507008 1_3:-35458290 Msa0116450:three_prime_UTR
GATACCATGGGAGTGTCGTT+CGG 0.508317 1_3:-35458492 Msa0116450:CDS
GACCGGAAACGCCACTCTGC+CGG 0.512557 1_3:-35458915 Msa0116450:CDS
AGATATGTATAGAGGAGAGA+TGG 0.512762 1_3:+35458220 None:intergenic
CCAACCTCCCATTTGTCGAC+TGG 0.514767 1_3:-35458425 Msa0116450:CDS
TAAAATAATGGTTGCATACA+GGG 0.515469 1_3:+35458371 None:intergenic
TCAGGCGGAGGCTGCAGTGA+CGG 0.518687 1_3:+35458459 None:intergenic
CTGCAGCCTCCGCCTGAGGG+TGG 0.519587 1_3:-35458453 Msa0116450:CDS
AAGGGTTGATTAGATGGTGG+TGG 0.521880 1_3:+35458825 None:intergenic
TTCAAGATACACCCTTAGAC+CGG 0.529755 1_3:-35458932 Msa0116450:CDS
CCGGCGCGACACCGTTCTCC+AGG 0.530698 1_3:-35459338 Msa0116450:five_prime_UTR
AACATACTAATAGTTCTGAT+AGG 0.533532 1_3:-35458336 Msa0116450:three_prime_UTR
GAGTATCCACCCTCAGGCGG+AGG 0.540027 1_3:+35458447 None:intergenic
GACAACCTCTGTTCTAGGTC+CGG 0.542074 1_3:-35459357 Msa0116450:five_prime_UTR
AGGTTGGAGTATCCACCCTC+AGG 0.543831 1_3:+35458441 None:intergenic
AGACACAAGGCAGGCATACA+AGG 0.545292 1_3:+35459386 None:intergenic
ATATATGTAGGGAATATAGT+AGG 0.551592 1_3:-35458516 Msa0116450:intron
GTAGAGTTGAGGCGTGGGTG+TGG 0.557491 1_3:-35459011 Msa0116450:five_prime_UTR
AACGGCGGCGTCCTGGAGAA+CGG 0.558689 1_3:+35459327 None:intergenic
TCGCATAGAATGGAAGTGGC+AGG 0.559621 1_3:+35459301 None:intergenic
GTTGAGGCGTGGGTGTGGTG+AGG 0.560632 1_3:-35459006 Msa0116450:five_prime_UTR
CCAGTCGACAAATGGGAGGT+TGG 0.561835 1_3:+35458425 None:intergenic
AACTTTCGATCATAAAACAA+AGG 0.564239 1_3:+35459421 None:intergenic
ATAGTACCGTGTTGACGAAG+TGG 0.572113 1_3:+35458796 None:intergenic
CCTGGAGAACGGTGTCGCGC+CGG 0.577030 1_3:+35459338 None:intergenic
CAACCAATAACAAGAATGCC+AGG 0.578455 1_3:-35458631 Msa0116450:intron
TTGTAGTAGAGTTGAGGCGT+GGG 0.580503 1_3:-35459016 Msa0116450:five_prime_UTR
GGCGTCGCATAGAATGGAAG+TGG 0.581345 1_3:+35459297 None:intergenic
GAGTGGCGTTTCCGGTCTAA+GGG 0.584406 1_3:+35458921 None:intergenic
TACAGCGTCGTCGCGCAGAA+CGG 0.586040 1_3:+35459273 None:intergenic
AATGGAAGTGGCAGGCAGAA+CGG 0.586179 1_3:+35459309 None:intergenic
GTCGGACTCAGACACAAGGC+AGG 0.593371 1_3:+35459377 None:intergenic
TTGACGAAGTGGACGCAGAA+GGG 0.593699 1_3:+35458807 None:intergenic
CCATATTTGTAGTAGAGTTG+AGG 0.596000 1_3:-35459022 Msa0116450:five_prime_UTR
GTTGACGAAGTGGACGCAGA+AGG 0.597332 1_3:+35458806 None:intergenic
TTTGTAGTAGAGTTGAGGCG+TGG 0.611994 1_3:-35459017 Msa0116450:five_prime_UTR
GGACCTAGAACAGAGGTTGT+CGG 0.613319 1_3:+35459359 None:intergenic
GGCGGAGGCTGCAGTGACGG+AGG 0.613435 1_3:+35458462 None:intergenic
GAATATAGTAGGAGATACCA+TGG 0.619077 1_3:-35458505 Msa0116450:CDS
CTGCGTCCACTTCGTCAACA+CGG 0.619782 1_3:-35458802 Msa0116450:intron
TTGCATACAGGGAGGCCCAA+AGG 0.621250 1_3:+35458382 None:intergenic
AGCGTCGTCGCGCAGAACGG+AGG 0.624495 1_3:+35459276 None:intergenic
TCGCGCCGGACCTAGAACAG+AGG 0.642209 1_3:+35459352 None:intergenic
AAAGATCATAATTCATAACA+AGG 0.646758 1_3:+35458243 None:intergenic
ACAAACAAGCCAAAGCTCGT+TGG 0.650557 1_3:-35458884 Msa0116450:CDS
ATTGCCGAACGACACTCCCA+TGG 0.650589 1_3:+35458488 None:intergenic
CAGAAGGGTTGATTAGATGG+TGG 0.659317 1_3:+35458822 None:intergenic
TTGGAGTATCCACCCTCAGG+CGG 0.670531 1_3:+35458444 None:intergenic
ATCCCCAGTCGACAAATGGG+AGG 0.671698 1_3:+35458421 None:intergenic
AACCTCCCATTTGTCGACTG+GGG 0.672189 1_3:-35458423 Msa0116450:CDS
GGTTGTCGGACTCAGACACA+AGG 0.683594 1_3:+35459373 None:intergenic
GGAAGTGGCAGGCAGAACGG+CGG 0.688489 1_3:+35459312 None:intergenic
AATATAGTAGGAGATACCAT+GGG 0.697591 1_3:-35458504 Msa0116450:CDS
TGTGTTGAATACCGGCAGAG+TGG 0.711189 1_3:+35458904 None:intergenic
AATAATGGTTGCATACAGGG+AGG 0.744657 1_3:+35458374 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGAATTAAAACTCAATTAA+TGG + chr1_3:35458536-35458555 None:intergenic 15.0%
!! ATTTATAGTTTCTTTCTTTA+TGG - chr1_3:35459046-35459065 Msa0116450:intron 15.0%
!! TTGAATTAAAACTCAATTAA+TGG + chr1_3:35458536-35458555 None:intergenic 15.0%
!! ATTTATAGTTTCTTTCTTTA+TGG - chr1_3:35459046-35459065 Msa0116450:intron 15.0%
!! CTAAGAAATGATAATTAATG+AGG + chr1_3:35458876-35458895 None:intergenic 20.0%
!! TTAACTCTCTTTGTATATTT+AGG - chr1_3:35458911-35458930 Msa0116450:CDS 20.0%
!! TAACTCTCTTTGTATATTTA+GGG - chr1_3:35458912-35458931 Msa0116450:CDS 20.0%
!! TCATTATAGCCATAAAATAA+TGG + chr1_3:35459268-35459287 None:intergenic 20.0%
!! AAAGATCATAATTCATAACA+AGG + chr1_3:35459384-35459403 None:intergenic 20.0%
!!! TCTTTTTTAGATATGTATAG+AGG + chr1_3:35459415-35459434 None:intergenic 20.0%
!! CTAAGAAATGATAATTAATG+AGG + chr1_3:35458876-35458895 None:intergenic 20.0%
!! TTAACTCTCTTTGTATATTT+AGG - chr1_3:35458911-35458930 Msa0116450:CDS 20.0%
!! TAACTCTCTTTGTATATTTA+GGG - chr1_3:35458912-35458931 Msa0116450:CDS 20.0%
!! TCATTATAGCCATAAAATAA+TGG + chr1_3:35459268-35459287 None:intergenic 20.0%
!! AAAGATCATAATTCATAACA+AGG + chr1_3:35459384-35459403 None:intergenic 20.0%
!!! TCTTTTTTAGATATGTATAG+AGG + chr1_3:35459415-35459434 None:intergenic 20.0%
! AACTTTCGATCATAAAACAA+AGG + chr1_3:35458206-35458225 None:intergenic 25.0%
! TCATACAAGTCATTTGTAAT+TGG - chr1_3:35458405-35458424 Msa0116450:exon 25.0%
!!! TCACAATAGTACTTTTTCAT+TGG + chr1_3:35458655-35458674 None:intergenic 25.0%
! ATCAGTAAATGAACAACTAT+CGG + chr1_3:35459028-35459047 None:intergenic 25.0%
! TATGTACTATGCATATATGT+AGG - chr1_3:35459096-35459115 Msa0116450:intron 25.0%
! ATGTACTATGCATATATGTA+GGG - chr1_3:35459097-35459116 Msa0116450:intron 25.0%
! ATATATGTAGGGAATATAGT+AGG - chr1_3:35459108-35459127 Msa0116450:intron 25.0%
! TAAAATAATGGTTGCATACA+GGG + chr1_3:35459256-35459275 None:intergenic 25.0%
! ATAAAATAATGGTTGCATAC+AGG + chr1_3:35459257-35459276 None:intergenic 25.0%
!!! TGTATGCAACCATTATTTTA+TGG - chr1_3:35459256-35459275 Msa0116450:five_prime_UTR 25.0%
!!! AACATACTAATAGTTCTGAT+AGG - chr1_3:35459288-35459307 Msa0116450:five_prime_UTR 25.0%
! ATAACAAGGTAACATTTAAC+AGG + chr1_3:35459370-35459389 None:intergenic 25.0%
! AACTTTCGATCATAAAACAA+AGG + chr1_3:35458206-35458225 None:intergenic 25.0%
! TCATACAAGTCATTTGTAAT+TGG - chr1_3:35458405-35458424 Msa0116450:exon 25.0%
!!! TCACAATAGTACTTTTTCAT+TGG + chr1_3:35458655-35458674 None:intergenic 25.0%
! ATCAGTAAATGAACAACTAT+CGG + chr1_3:35459028-35459047 None:intergenic 25.0%
! TATGTACTATGCATATATGT+AGG - chr1_3:35459096-35459115 Msa0116450:intron 25.0%
! ATGTACTATGCATATATGTA+GGG - chr1_3:35459097-35459116 Msa0116450:intron 25.0%
! ATATATGTAGGGAATATAGT+AGG - chr1_3:35459108-35459127 Msa0116450:intron 25.0%
! TAAAATAATGGTTGCATACA+GGG + chr1_3:35459256-35459275 None:intergenic 25.0%
! ATAAAATAATGGTTGCATAC+AGG + chr1_3:35459257-35459276 None:intergenic 25.0%
!!! TGTATGCAACCATTATTTTA+TGG - chr1_3:35459256-35459275 Msa0116450:five_prime_UTR 25.0%
!!! AACATACTAATAGTTCTGAT+AGG - chr1_3:35459288-35459307 Msa0116450:five_prime_UTR 25.0%
! ATAACAAGGTAACATTTAAC+AGG + chr1_3:35459370-35459389 None:intergenic 25.0%
TACAAGTCATTTGTAATTGG+CGG - chr1_3:35458408-35458427 Msa0116450:exon 30.0%
ATGAAACAAGAGCAAAGATA+AGG + chr1_3:35458479-35458498 None:intergenic 30.0%
! ATTTAGGGATTTTGTCGTTA+TGG - chr1_3:35458927-35458946 Msa0116450:CDS 30.0%
TCAGTAAATGAACAACTATC+GGG + chr1_3:35459027-35459046 None:intergenic 30.0%
AATATAGTAGGAGATACCAT+GGG - chr1_3:35459120-35459139 Msa0116450:intron 30.0%
!!! CTGATTTTGAGTTTCTTTAC+TGG - chr1_3:35459317-35459336 Msa0116450:five_prime_UTR 30.0%
! TACTGGTTATTACAACTACT+AGG - chr1_3:35459334-35459353 Msa0116450:five_prime_UTR 30.0%
TACAAGTCATTTGTAATTGG+CGG - chr1_3:35458408-35458427 Msa0116450:exon 30.0%
ATGAAACAAGAGCAAAGATA+AGG + chr1_3:35458479-35458498 None:intergenic 30.0%
! ATTTAGGGATTTTGTCGTTA+TGG - chr1_3:35458927-35458946 Msa0116450:CDS 30.0%
TCAGTAAATGAACAACTATC+GGG + chr1_3:35459027-35459046 None:intergenic 30.0%
AATATAGTAGGAGATACCAT+GGG - chr1_3:35459120-35459139 Msa0116450:intron 30.0%
!!! CTGATTTTGAGTTTCTTTAC+TGG - chr1_3:35459317-35459336 Msa0116450:five_prime_UTR 30.0%
! TACTGGTTATTACAACTACT+AGG - chr1_3:35459334-35459353 Msa0116450:five_prime_UTR 30.0%
ATGTGAGCGTAGAAAGAAAT+TGG + chr1_3:35458377-35458396 None:intergenic 35.0%
TTCTTTCTACGCTCACATTA+AGG - chr1_3:35458379-35458398 Msa0116450:three_prime_UTR 35.0%
AGCAAAGATAAGGATTGTTC+GGG + chr1_3:35458469-35458488 None:intergenic 35.0%
GAGCAAAGATAAGGATTGTT+CGG + chr1_3:35458470-35458489 None:intergenic 35.0%
! CTCTTGTTTCATGTTCACAT+GGG - chr1_3:35458487-35458506 Msa0116450:CDS 35.0%
CCTCAACTCTACTACAAATA+TGG + chr1_3:35458605-35458624 None:intergenic 35.0%
CCATATTTGTAGTAGAGTTG+AGG - chr1_3:35458602-35458621 Msa0116450:intron 35.0%
CAGTAAATGAACAACTATCG+GGG + chr1_3:35459026-35459045 None:intergenic 35.0%
GAATATAGTAGGAGATACCA+TGG - chr1_3:35459119-35459138 Msa0116450:intron 35.0%
AGATTTGTGCACATTACCTT+TGG - chr1_3:35459226-35459245 Msa0116450:intron 35.0%
GATTTGTGCACATTACCTTT+GGG - chr1_3:35459227-35459246 Msa0116450:intron 35.0%
AGATATGTATAGAGGAGAGA+TGG + chr1_3:35459407-35459426 None:intergenic 35.0%
ATGTGAGCGTAGAAAGAAAT+TGG + chr1_3:35458377-35458396 None:intergenic 35.0%
TTCTTTCTACGCTCACATTA+AGG - chr1_3:35458379-35458398 Msa0116450:three_prime_UTR 35.0%
AGCAAAGATAAGGATTGTTC+GGG + chr1_3:35458469-35458488 None:intergenic 35.0%
GAGCAAAGATAAGGATTGTT+CGG + chr1_3:35458470-35458489 None:intergenic 35.0%
! CTCTTGTTTCATGTTCACAT+GGG - chr1_3:35458487-35458506 Msa0116450:CDS 35.0%
CCTCAACTCTACTACAAATA+TGG + chr1_3:35458605-35458624 None:intergenic 35.0%
CCATATTTGTAGTAGAGTTG+AGG - chr1_3:35458602-35458621 Msa0116450:intron 35.0%
CAGTAAATGAACAACTATCG+GGG + chr1_3:35459026-35459045 None:intergenic 35.0%
GAATATAGTAGGAGATACCA+TGG - chr1_3:35459119-35459138 Msa0116450:intron 35.0%
AGATTTGTGCACATTACCTT+TGG - chr1_3:35459226-35459245 Msa0116450:intron 35.0%
GATTTGTGCACATTACCTTT+GGG - chr1_3:35459227-35459246 Msa0116450:intron 35.0%
AGATATGTATAGAGGAGAGA+TGG + chr1_3:35459407-35459426 None:intergenic 35.0%
! GCTCTTGTTTCATGTTCACA+TGG - chr1_3:35458486-35458505 Msa0116450:CDS 40.0%
TTCAAGATACACCCTTAGAC+CGG - chr1_3:35458692-35458711 Msa0116450:CDS 40.0%
! GGCTTGTTTGTGTTGAATAC+CGG + chr1_3:35458731-35458750 None:intergenic 40.0%
! TGGAATAGAGTTCTGGTGTT+CGG + chr1_3:35458782-35458801 None:intergenic 40.0%
CAACCAATAACAAGAATGCC+AGG - chr1_3:35458993-35459012 Msa0116450:exon 40.0%
! TCACCTGGCATTCTTGTTAT+TGG + chr1_3:35458999-35459018 None:intergenic 40.0%
AATAATGGTTGCATACAGGG+AGG + chr1_3:35459253-35459272 None:intergenic 40.0%
! GCTCTTGTTTCATGTTCACA+TGG - chr1_3:35458486-35458505 Msa0116450:CDS 40.0%
TTCAAGATACACCCTTAGAC+CGG - chr1_3:35458692-35458711 Msa0116450:CDS 40.0%
! GGCTTGTTTGTGTTGAATAC+CGG + chr1_3:35458731-35458750 None:intergenic 40.0%
! TGGAATAGAGTTCTGGTGTT+CGG + chr1_3:35458782-35458801 None:intergenic 40.0%
CAACCAATAACAAGAATGCC+AGG - chr1_3:35458993-35459012 Msa0116450:exon 40.0%
! TCACCTGGCATTCTTGTTAT+TGG + chr1_3:35458999-35459018 None:intergenic 40.0%
AATAATGGTTGCATACAGGG+AGG + chr1_3:35459253-35459272 None:intergenic 40.0%
GGCAGGCATACAAGGAAAAT+GGG + chr1_3:35458233-35458252 None:intergenic 45.0%
AGGCAGGCATACAAGGAAAA+TGG + chr1_3:35458234-35458253 None:intergenic 45.0%
TTGCAACTTCAAGCTTCGTG+CGG + chr1_3:35458439-35458458 None:intergenic 45.0%
TTTGTAGTAGAGTTGAGGCG+TGG - chr1_3:35458607-35458626 Msa0116450:intron 45.0%
TTGTAGTAGAGTTGAGGCGT+GGG - chr1_3:35458608-35458627 Msa0116450:intron 45.0%
ACAAACAAGCCAAAGCTCGT+TGG - chr1_3:35458740-35458759 Msa0116450:intron 45.0%
! GGAATAGAGTTCTGGTGTTC+GGG + chr1_3:35458781-35458800 None:intergenic 45.0%
!! ATGGTGGTGGAATAGAGTTC+TGG + chr1_3:35458789-35458808 None:intergenic 45.0%
! AAGGGTTGATTAGATGGTGG+TGG + chr1_3:35458802-35458821 None:intergenic 45.0%
! CAGAAGGGTTGATTAGATGG+TGG + chr1_3:35458805-35458824 None:intergenic 45.0%
! ACGCAGAAGGGTTGATTAGA+TGG + chr1_3:35458808-35458827 None:intergenic 45.0%
! ATAGTACCGTGTTGACGAAG+TGG + chr1_3:35458831-35458850 None:intergenic 45.0%
TGCTGCTGCTGTTGTGTATT+GGG + chr1_3:35458975-35458994 None:intergenic 45.0%
TTGCTGCTGCTGTTGTGTAT+TGG + chr1_3:35458976-35458995 None:intergenic 45.0%
TCTATCCCCAGTCGACAAAT+GGG + chr1_3:35459209-35459228 None:intergenic 45.0%
ATCTATCCCCAGTCGACAAA+TGG + chr1_3:35459210-35459229 None:intergenic 45.0%
GGCAGGCATACAAGGAAAAT+GGG + chr1_3:35458233-35458252 None:intergenic 45.0%
AGGCAGGCATACAAGGAAAA+TGG + chr1_3:35458234-35458253 None:intergenic 45.0%
TTGCAACTTCAAGCTTCGTG+CGG + chr1_3:35458439-35458458 None:intergenic 45.0%
TTTGTAGTAGAGTTGAGGCG+TGG - chr1_3:35458607-35458626 Msa0116450:intron 45.0%
TTGTAGTAGAGTTGAGGCGT+GGG - chr1_3:35458608-35458627 Msa0116450:intron 45.0%
ACAAACAAGCCAAAGCTCGT+TGG - chr1_3:35458740-35458759 Msa0116450:intron 45.0%
! GGAATAGAGTTCTGGTGTTC+GGG + chr1_3:35458781-35458800 None:intergenic 45.0%
!! ATGGTGGTGGAATAGAGTTC+TGG + chr1_3:35458789-35458808 None:intergenic 45.0%
! AAGGGTTGATTAGATGGTGG+TGG + chr1_3:35458802-35458821 None:intergenic 45.0%
! CAGAAGGGTTGATTAGATGG+TGG + chr1_3:35458805-35458824 None:intergenic 45.0%
! ACGCAGAAGGGTTGATTAGA+TGG + chr1_3:35458808-35458827 None:intergenic 45.0%
! ATAGTACCGTGTTGACGAAG+TGG + chr1_3:35458831-35458850 None:intergenic 45.0%
TGCTGCTGCTGTTGTGTATT+GGG + chr1_3:35458975-35458994 None:intergenic 45.0%
TTGCTGCTGCTGTTGTGTAT+TGG + chr1_3:35458976-35458995 None:intergenic 45.0%
TCTATCCCCAGTCGACAAAT+GGG + chr1_3:35459209-35459228 None:intergenic 45.0%
ATCTATCCCCAGTCGACAAA+TGG + chr1_3:35459210-35459229 None:intergenic 45.0%
AGACACAAGGCAGGCATACA+AGG + chr1_3:35458241-35458260 None:intergenic 50.0%
AGTCCGACAACCTCTGTTCT+AGG - chr1_3:35458262-35458281 Msa0116450:three_prime_UTR 50.0%
GGACCTAGAACAGAGGTTGT+CGG + chr1_3:35458268-35458287 None:intergenic 50.0%
! GACAACCTCTGTTCTAGGTC+CGG - chr1_3:35458267-35458286 Msa0116450:three_prime_UTR 50.0%
AATGGAAGTGGCAGGCAGAA+CGG + chr1_3:35458318-35458337 None:intergenic 50.0%
TCGCATAGAATGGAAGTGGC+AGG + chr1_3:35458326-35458345 None:intergenic 50.0%
TGTGTTGAATACCGGCAGAG+TGG + chr1_3:35458723-35458742 None:intergenic 50.0%
GGAATCGTACCAACGAGCTT+TGG + chr1_3:35458752-35458771 None:intergenic 50.0%
! GTTCTGGTGTTCGGGTTTGA+AGG + chr1_3:35458773-35458792 None:intergenic 50.0%
TTGACGAAGTGGACGCAGAA+GGG + chr1_3:35458820-35458839 None:intergenic 50.0%
!! GATACCATGGGAGTGTCGTT+CGG - chr1_3:35459132-35459151 Msa0116450:intron 50.0%
! CAACCTCCCATTTGTCGACT+GGG - chr1_3:35459200-35459219 Msa0116450:intron 50.0%
! AACCTCCCATTTGTCGACTG+GGG - chr1_3:35459201-35459220 Msa0116450:intron 50.0%
AGACACAAGGCAGGCATACA+AGG + chr1_3:35458241-35458260 None:intergenic 50.0%
AGTCCGACAACCTCTGTTCT+AGG - chr1_3:35458262-35458281 Msa0116450:three_prime_UTR 50.0%
GGACCTAGAACAGAGGTTGT+CGG + chr1_3:35458268-35458287 None:intergenic 50.0%
! GACAACCTCTGTTCTAGGTC+CGG - chr1_3:35458267-35458286 Msa0116450:three_prime_UTR 50.0%
AATGGAAGTGGCAGGCAGAA+CGG + chr1_3:35458318-35458337 None:intergenic 50.0%
TCGCATAGAATGGAAGTGGC+AGG + chr1_3:35458326-35458345 None:intergenic 50.0%
TGTGTTGAATACCGGCAGAG+TGG + chr1_3:35458723-35458742 None:intergenic 50.0%
GGAATCGTACCAACGAGCTT+TGG + chr1_3:35458752-35458771 None:intergenic 50.0%
! GTTCTGGTGTTCGGGTTTGA+AGG + chr1_3:35458773-35458792 None:intergenic 50.0%
TTGACGAAGTGGACGCAGAA+GGG + chr1_3:35458820-35458839 None:intergenic 50.0%
!! GATACCATGGGAGTGTCGTT+CGG - chr1_3:35459132-35459151 Msa0116450:intron 50.0%
! CAACCTCCCATTTGTCGACT+GGG - chr1_3:35459200-35459219 Msa0116450:intron 50.0%
! AACCTCCCATTTGTCGACTG+GGG - chr1_3:35459201-35459220 Msa0116450:intron 50.0%
GGTTGTCGGACTCAGACACA+AGG + chr1_3:35458254-35458273 None:intergenic 55.0%
GGCGTCGCATAGAATGGAAG+TGG + chr1_3:35458330-35458349 None:intergenic 55.0%
!! AACGGAGGCGTCGCATAGAA+TGG + chr1_3:35458336-35458355 None:intergenic 55.0%
CAACTTCAAGCTTCGTGCGG+TGG + chr1_3:35458436-35458455 None:intergenic 55.0%
GAGTGGCGTTTCCGGTCTAA+GGG + chr1_3:35458706-35458725 None:intergenic 55.0%
AGAGTGGCGTTTCCGGTCTA+AGG + chr1_3:35458707-35458726 None:intergenic 55.0%
GTTGACGAAGTGGACGCAGA+AGG + chr1_3:35458821-35458840 None:intergenic 55.0%
CTGCGTCCACTTCGTCAACA+CGG - chr1_3:35458822-35458841 Msa0116450:CDS 55.0%
AACTATCGGGGTAGCTCACC+TGG + chr1_3:35459014-35459033 None:intergenic 55.0%
ATTGCCGAACGACACTCCCA+TGG + chr1_3:35459139-35459158 None:intergenic 55.0%
! TTGGAGTATCCACCCTCAGG+CGG + chr1_3:35459183-35459202 None:intergenic 55.0%
! AGGTTGGAGTATCCACCCTC+AGG + chr1_3:35459186-35459205 None:intergenic 55.0%
CCAGTCGACAAATGGGAGGT+TGG + chr1_3:35459202-35459221 None:intergenic 55.0%
CCAACCTCCCATTTGTCGAC+TGG - chr1_3:35459199-35459218 Msa0116450:intron 55.0%
ATCCCCAGTCGACAAATGGG+AGG + chr1_3:35459206-35459225 None:intergenic 55.0%
TTGCATACAGGGAGGCCCAA+AGG + chr1_3:35459245-35459264 None:intergenic 55.0%
GGTTGTCGGACTCAGACACA+AGG + chr1_3:35458254-35458273 None:intergenic 55.0%
GGCGTCGCATAGAATGGAAG+TGG + chr1_3:35458330-35458349 None:intergenic 55.0%
!! AACGGAGGCGTCGCATAGAA+TGG + chr1_3:35458336-35458355 None:intergenic 55.0%
CAACTTCAAGCTTCGTGCGG+TGG + chr1_3:35458436-35458455 None:intergenic 55.0%
GAGTGGCGTTTCCGGTCTAA+GGG + chr1_3:35458706-35458725 None:intergenic 55.0%
AGAGTGGCGTTTCCGGTCTA+AGG + chr1_3:35458707-35458726 None:intergenic 55.0%
GTTGACGAAGTGGACGCAGA+AGG + chr1_3:35458821-35458840 None:intergenic 55.0%
CTGCGTCCACTTCGTCAACA+CGG - chr1_3:35458822-35458841 Msa0116450:CDS 55.0%
AACTATCGGGGTAGCTCACC+TGG + chr1_3:35459014-35459033 None:intergenic 55.0%
ATTGCCGAACGACACTCCCA+TGG + chr1_3:35459139-35459158 None:intergenic 55.0%
! TTGGAGTATCCACCCTCAGG+CGG + chr1_3:35459183-35459202 None:intergenic 55.0%
! AGGTTGGAGTATCCACCCTC+AGG + chr1_3:35459186-35459205 None:intergenic 55.0%
CCAGTCGACAAATGGGAGGT+TGG + chr1_3:35459202-35459221 None:intergenic 55.0%
CCAACCTCCCATTTGTCGAC+TGG - chr1_3:35459199-35459218 Msa0116450:intron 55.0%
ATCCCCAGTCGACAAATGGG+AGG + chr1_3:35459206-35459225 None:intergenic 55.0%
TTGCATACAGGGAGGCCCAA+AGG + chr1_3:35459245-35459264 None:intergenic 55.0%
GTCGGACTCAGACACAAGGC+AGG + chr1_3:35458250-35458269 None:intergenic 60.0%
TACAGCGTCGTCGCGCAGAA+CGG + chr1_3:35458354-35458373 None:intergenic 60.0%
GTAGAGTTGAGGCGTGGGTG+TGG - chr1_3:35458613-35458632 Msa0116450:intron 60.0%
TACCGGCAGAGTGGCGTTTC+CGG + chr1_3:35458714-35458733 None:intergenic 60.0%
GTCGGACTCAGACACAAGGC+AGG + chr1_3:35458250-35458269 None:intergenic 60.0%
TACAGCGTCGTCGCGCAGAA+CGG + chr1_3:35458354-35458373 None:intergenic 60.0%
GTAGAGTTGAGGCGTGGGTG+TGG - chr1_3:35458613-35458632 Msa0116450:intron 60.0%
TACCGGCAGAGTGGCGTTTC+CGG + chr1_3:35458714-35458733 None:intergenic 60.0%
TCGCGCCGGACCTAGAACAG+AGG + chr1_3:35458275-35458294 None:intergenic 65.0%
AACGGCGGCGTCCTGGAGAA+CGG + chr1_3:35458300-35458319 None:intergenic 65.0%
GGAAGTGGCAGGCAGAACGG+CGG + chr1_3:35458315-35458334 None:intergenic 65.0%
GTTGAGGCGTGGGTGTGGTG+AGG - chr1_3:35458618-35458637 Msa0116450:intron 65.0%
AGGCGTGGGTGTGGTGAGGA+GGG - chr1_3:35458622-35458641 Msa0116450:intron 65.0%
GACCGGAAACGCCACTCTGC+CGG - chr1_3:35458709-35458728 Msa0116450:intron 65.0%
TCAGGCGGAGGCTGCAGTGA+CGG + chr1_3:35459168-35459187 None:intergenic 65.0%
TCACTGCAGCCTCCGCCTGA+GGG - chr1_3:35459168-35459187 Msa0116450:intron 65.0%
GAGTATCCACCCTCAGGCGG+AGG + chr1_3:35459180-35459199 None:intergenic 65.0%
TCGCGCCGGACCTAGAACAG+AGG + chr1_3:35458275-35458294 None:intergenic 65.0%
AACGGCGGCGTCCTGGAGAA+CGG + chr1_3:35458300-35458319 None:intergenic 65.0%
GGAAGTGGCAGGCAGAACGG+CGG + chr1_3:35458315-35458334 None:intergenic 65.0%
GTTGAGGCGTGGGTGTGGTG+AGG - chr1_3:35458618-35458637 Msa0116450:intron 65.0%
AGGCGTGGGTGTGGTGAGGA+GGG - chr1_3:35458622-35458641 Msa0116450:intron 65.0%
GACCGGAAACGCCACTCTGC+CGG - chr1_3:35458709-35458728 Msa0116450:intron 65.0%
TCAGGCGGAGGCTGCAGTGA+CGG + chr1_3:35459168-35459187 None:intergenic 65.0%
TCACTGCAGCCTCCGCCTGA+GGG - chr1_3:35459168-35459187 Msa0116450:intron 65.0%
GAGTATCCACCCTCAGGCGG+AGG + chr1_3:35459180-35459199 None:intergenic 65.0%
!! CCTGGAGAACGGTGTCGCGC+CGG + chr1_3:35458289-35458308 None:intergenic 70.0%
AGCGTCGTCGCGCAGAACGG+AGG + chr1_3:35458351-35458370 None:intergenic 70.0%
GAGGCGTGGGTGTGGTGAGG+AGG - chr1_3:35458621-35458640 Msa0116450:intron 70.0%
GGCGTGGGTGTGGTGAGGAG+GGG - chr1_3:35458623-35458642 Msa0116450:intron 70.0%
GTCACTGCAGCCTCCGCCTG+AGG - chr1_3:35459167-35459186 Msa0116450:intron 70.0%
!! CCTGGAGAACGGTGTCGCGC+CGG + chr1_3:35458289-35458308 None:intergenic 70.0%
AGCGTCGTCGCGCAGAACGG+AGG + chr1_3:35458351-35458370 None:intergenic 70.0%
GAGGCGTGGGTGTGGTGAGG+AGG - chr1_3:35458621-35458640 Msa0116450:intron 70.0%
GGCGTGGGTGTGGTGAGGAG+GGG - chr1_3:35458623-35458642 Msa0116450:intron 70.0%
GTCACTGCAGCCTCCGCCTG+AGG - chr1_3:35459167-35459186 Msa0116450:intron 70.0%
CCGGCGCGACACCGTTCTCC+AGG - chr1_3:35458286-35458305 Msa0116450:three_prime_UTR 75.0%
CAGGCAGAACGGCGGCGTCC+TGG + chr1_3:35458307-35458326 None:intergenic 75.0%
GGCGGAGGCTGCAGTGACGG+AGG + chr1_3:35459165-35459184 None:intergenic 75.0%
CTGCAGCCTCCGCCTGAGGG+TGG - chr1_3:35459171-35459190 Msa0116450:intron 75.0%
CCGGCGCGACACCGTTCTCC+AGG - chr1_3:35458286-35458305 Msa0116450:three_prime_UTR 75.0%
CAGGCAGAACGGCGGCGTCC+TGG + chr1_3:35458307-35458326 None:intergenic 75.0%
GGCGGAGGCTGCAGTGACGG+AGG + chr1_3:35459165-35459184 None:intergenic 75.0%
CTGCAGCCTCCGCCTGAGGG+TGG - chr1_3:35459171-35459190 Msa0116450:intron 75.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 35458199 35459447 35458199 ID=Msa0116450;Name=Msa0116450
chr1_3 mRNA 35458199 35459447 35458199 ID=Msa0116450-mRNA-1;Parent=Msa0116450;Name=Msa0116450-mRNA-1;_AED=0.01;_eAED=0.01;_QI=266|1|1|1|1|1|4|220|128
chr1_3 exon 35458199 35458528 35458199 ID=Msa0116450-mRNA-1:exon:11349;Parent=Msa0116450-mRNA-1
chr1_3 exon 35458632 35458713 35458632 ID=Msa0116450-mRNA-1:exon:11348;Parent=Msa0116450-mRNA-1
chr1_3 exon 35458803 35459061 35458803 ID=Msa0116450-mRNA-1:exon:11347;Parent=Msa0116450-mRNA-1
chr1_3 exon 35459246 35459447 35459246 ID=Msa0116450-mRNA-1:exon:11346;Parent=Msa0116450-mRNA-1
chr1_3 five_prime_UTR 35459246 35459447 35459246 ID=Msa0116450-mRNA-1:five_prime_utr;Parent=Msa0116450-mRNA-1
chr1_3 five_prime_UTR 35458998 35459061 35458998 ID=Msa0116450-mRNA-1:five_prime_utr;Parent=Msa0116450-mRNA-1
chr1_3 CDS 35458803 35458997 35458803 ID=Msa0116450-mRNA-1:cds;Parent=Msa0116450-mRNA-1
chr1_3 CDS 35458632 35458713 35458632 ID=Msa0116450-mRNA-1:cds;Parent=Msa0116450-mRNA-1
chr1_3 CDS 35458419 35458528 35458419 ID=Msa0116450-mRNA-1:cds;Parent=Msa0116450-mRNA-1
chr1_3 three_prime_UTR 35458199 35458418 35458199 ID=Msa0116450-mRNA-1:three_prime_utr;Parent=Msa0116450-mRNA-1
Gene Sequence

>Msa0116450

ATGCCAATGAAAAAGTACTATTGTGAGTATTGCGATAAGCAATTTCAAGATACACCCTTAGACCGGAAACGCCACTCTGCCGGTATTCAACACAAACAAGCCAAAGCTCGTTGGTACGATTCCTTCAAACCCGAACACCAGAACTCTATTCCACCACCATCTAATCAACCCTTCTGCGTCCACTTCGTCAACACGGGATTTTGTCGTTATGGCGATTCTTGCAAATATTTTCATCCCAATACACAACAGCAGCAGCAACCAATAACAAGAATGCCAGGGAATATAGTAGGAGATACCATGGGAGTGTCGTTCGGCAATTTGCCTCCGTCACTGCAGCCTCCGCCTGAGGGTGGATACTCCAACCTCCCATTTGTCGACTGGGGATAG
Protein sequence

>Msa0116450

MPMKKYYCEYCDKQFQDTPLDRKRHSAGIQHKQAKARWYDSFKPEHQNSIPPPSNQPFCVHFVNTGFCRYGDSCKYFHPNTQQQQQPITRMPGNIVGDTMGVSFGNLPPSLQPPPEGGYSNLPFVDWG*