AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0116490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0116490 MtrunA17_Chr1g0193071 86.719 128 11 2 1 128 1 122 1.76e-70 207
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0116490 AT5G26749.1 40.136 147 69 2 1 128 1 147 5.47e-32 110
Msa0116490 AT5G26749.2 35.542 166 69 3 1 128 1 166 1.87e-27 99.8

Find 88 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACCTGGCATTCTTGTTAT+TGG 0.166998 1_3:+35489277 None:intergenic
TGCTGCTGCTGTTGTGTATT+GGG 0.205632 1_3:+35489301 None:intergenic
TACCGGCAGAGTGGCGTTTC+CGG 0.248184 1_3:+35489562 None:intergenic
AGGCAGGCATACAAGGAAAA+TGG 0.254879 1_3:+35490042 None:intergenic
CCTCAACTCTACTACAAATA+TGG 0.258437 1_3:+35489671 None:intergenic
AGAGTGGCGTTTCCGGTCTA+AGG 0.283984 1_3:+35489569 None:intergenic
TTCTTTCTACGCTCACATTA+AGG 0.284952 1_3:-35489894 Msa0116490:intron
TCATTATAGCCATAAAATAA+TGG 0.316834 1_3:+35489008 None:intergenic
TTGCTGCTGCTGTTGTGTAT+TGG 0.317673 1_3:+35489300 None:intergenic
GGAATAGAGTTCTGGTGTTC+GGG 0.348282 1_3:+35489495 None:intergenic
AGATTTGTGCACATTACCTT+TGG 0.358949 1_3:-35489047 Msa0116490:three_prime_UTR
ATAACAAGGTAACATTTAAC+AGG 0.361622 1_3:+35488906 None:intergenic
ATGTGAGCGTAGAAAGAAAT+TGG 0.367973 1_3:+35489899 None:intergenic
TGGAATAGAGTTCTGGTGTT+CGG 0.377509 1_3:+35489494 None:intergenic
AGTCCGACAACCTCTGTTCT+AGG 0.387209 1_3:-35490011 Msa0116490:five_prime_UTR
ATCTATCCCCAGTCGACAAA+TGG 0.397656 1_3:+35489066 None:intergenic
AGGCGTGGGTGTGGTGAGGA+GGG 0.402639 1_3:-35489651 Msa0116490:five_prime_UTR
GAGGCGTGGGTGTGGTGAGG+AGG 0.413445 1_3:-35489652 Msa0116490:five_prime_UTR
CAGGCAGAACGGCGGCGTCC+TGG 0.417159 1_3:+35489969 None:intergenic
ATGGTGGTGGAATAGAGTTC+TGG 0.430967 1_3:+35489487 None:intergenic
GGAATCGTACCAACGAGCTT+TGG 0.434321 1_3:+35489524 None:intergenic
ATAAAATAATGGTTGCATAC+AGG 0.435792 1_3:+35489019 None:intergenic
GATTTGTGCACATTACCTTT+GGG 0.444503 1_3:-35489046 Msa0116490:three_prime_UTR
CAACCTCCCATTTGTCGACT+GGG 0.457123 1_3:-35489073 Msa0116490:CDS
GTCACTGCAGCCTCCGCCTG+AGG 0.458331 1_3:-35489106 Msa0116490:CDS
TCACTGCAGCCTCCGCCTGA+GGG 0.463350 1_3:-35489105 Msa0116490:CDS
GTTCTGGTGTTCGGGTTTGA+AGG 0.464430 1_3:+35489503 None:intergenic
AACGGAGGCGTCGCATAGAA+TGG 0.465306 1_3:+35489940 None:intergenic
ACGCAGAAGGGTTGATTAGA+TGG 0.466779 1_3:+35489468 None:intergenic
GGCAGGCATACAAGGAAAAT+GGG 0.474158 1_3:+35490043 None:intergenic
GGCTTGTTTGTGTTGAATAC+CGG 0.496651 1_3:+35489545 None:intergenic
GGCGTGGGTGTGGTGAGGAG+GGG 0.501306 1_3:-35489650 Msa0116490:five_prime_UTR
TCTATCCCCAGTCGACAAAT+GGG 0.506048 1_3:+35489067 None:intergenic
TACTGGTTATTACAACTACT+AGG 0.507008 1_3:-35488939 Msa0116490:three_prime_UTR
GATACCATGGGAGTGTCGTT+CGG 0.508317 1_3:-35489141 Msa0116490:CDS
GACCGGAAACGCCACTCTGC+CGG 0.512557 1_3:-35489564 Msa0116490:CDS
AGATATGTATAGAGGAGAGA+TGG 0.512762 1_3:+35488869 None:intergenic
CCAACCTCCCATTTGTCGAC+TGG 0.514767 1_3:-35489074 Msa0116490:CDS
TAAAATAATGGTTGCATACA+GGG 0.515469 1_3:+35489020 None:intergenic
TCAGGCGGAGGCTGCAGTGA+CGG 0.518687 1_3:+35489108 None:intergenic
CTGCAGCCTCCGCCTGAGGG+TGG 0.519587 1_3:-35489102 Msa0116490:CDS
AAGGGTTGATTAGATGGTGG+TGG 0.521880 1_3:+35489474 None:intergenic
TTCAAGATACACCCTTAGAC+CGG 0.529755 1_3:-35489581 Msa0116490:CDS
CCGGCGCGACACCGTTCTCC+AGG 0.530698 1_3:-35489987 Msa0116490:five_prime_UTR
AACATACTAATAGTTCTGAT+AGG 0.533532 1_3:-35488985 Msa0116490:three_prime_UTR
GAGTATCCACCCTCAGGCGG+AGG 0.540027 1_3:+35489096 None:intergenic
GACAACCTCTGTTCTAGGTC+CGG 0.542074 1_3:-35490006 Msa0116490:five_prime_UTR
AGGTTGGAGTATCCACCCTC+AGG 0.543831 1_3:+35489090 None:intergenic
AGACACAAGGCAGGCATACA+AGG 0.545292 1_3:+35490035 None:intergenic
ATATATGTAGGGAATATAGT+AGG 0.551592 1_3:-35489165 Msa0116490:intron
GTAGAGTTGAGGCGTGGGTG+TGG 0.557491 1_3:-35489660 Msa0116490:five_prime_UTR
AACGGCGGCGTCCTGGAGAA+CGG 0.558689 1_3:+35489976 None:intergenic
TCGCATAGAATGGAAGTGGC+AGG 0.559621 1_3:+35489950 None:intergenic
GTTGAGGCGTGGGTGTGGTG+AGG 0.560632 1_3:-35489655 Msa0116490:five_prime_UTR
CCAGTCGACAAATGGGAGGT+TGG 0.561835 1_3:+35489074 None:intergenic
AACTTTCGATCATAAAACAA+AGG 0.564239 1_3:+35490070 None:intergenic
ATAGTACCGTGTTGACGAAG+TGG 0.572113 1_3:+35489445 None:intergenic
CCTGGAGAACGGTGTCGCGC+CGG 0.577030 1_3:+35489987 None:intergenic
CAACCAATAACAAGAATGCC+AGG 0.578455 1_3:-35489280 Msa0116490:intron
TTGTAGTAGAGTTGAGGCGT+GGG 0.580503 1_3:-35489665 Msa0116490:five_prime_UTR
GGCGTCGCATAGAATGGAAG+TGG 0.581345 1_3:+35489946 None:intergenic
GAGTGGCGTTTCCGGTCTAA+GGG 0.584406 1_3:+35489570 None:intergenic
TACAGCGTCGTCGCGCAGAA+CGG 0.586040 1_3:+35489922 None:intergenic
AATGGAAGTGGCAGGCAGAA+CGG 0.586179 1_3:+35489958 None:intergenic
GTCGGACTCAGACACAAGGC+AGG 0.593371 1_3:+35490026 None:intergenic
TTGACGAAGTGGACGCAGAA+GGG 0.593699 1_3:+35489456 None:intergenic
CCATATTTGTAGTAGAGTTG+AGG 0.596000 1_3:-35489671 Msa0116490:five_prime_UTR
GTTGACGAAGTGGACGCAGA+AGG 0.597332 1_3:+35489455 None:intergenic
TTTGTAGTAGAGTTGAGGCG+TGG 0.611994 1_3:-35489666 Msa0116490:five_prime_UTR
GGACCTAGAACAGAGGTTGT+CGG 0.613319 1_3:+35490008 None:intergenic
GGCGGAGGCTGCAGTGACGG+AGG 0.613435 1_3:+35489111 None:intergenic
GAATATAGTAGGAGATACCA+TGG 0.619077 1_3:-35489154 Msa0116490:CDS
CTGCGTCCACTTCGTCAACA+CGG 0.619782 1_3:-35489451 Msa0116490:intron
TTGCATACAGGGAGGCCCAA+AGG 0.621250 1_3:+35489031 None:intergenic
AGCGTCGTCGCGCAGAACGG+AGG 0.624495 1_3:+35489925 None:intergenic
TCGCGCCGGACCTAGAACAG+AGG 0.642209 1_3:+35490001 None:intergenic
AAAGATCATAATTCATAACA+AGG 0.646758 1_3:+35488892 None:intergenic
ACAAACAAGCCAAAGCTCGT+TGG 0.650557 1_3:-35489533 Msa0116490:CDS
ATTGCCGAACGACACTCCCA+TGG 0.650589 1_3:+35489137 None:intergenic
CAGAAGGGTTGATTAGATGG+TGG 0.659317 1_3:+35489471 None:intergenic
TTGGAGTATCCACCCTCAGG+CGG 0.670531 1_3:+35489093 None:intergenic
ATCCCCAGTCGACAAATGGG+AGG 0.671698 1_3:+35489070 None:intergenic
AACCTCCCATTTGTCGACTG+GGG 0.672189 1_3:-35489072 Msa0116490:CDS
GGTTGTCGGACTCAGACACA+AGG 0.683594 1_3:+35490022 None:intergenic
GGAAGTGGCAGGCAGAACGG+CGG 0.688489 1_3:+35489961 None:intergenic
AATATAGTAGGAGATACCAT+GGG 0.697591 1_3:-35489153 Msa0116490:CDS
TGTGTTGAATACCGGCAGAG+TGG 0.711189 1_3:+35489553 None:intergenic
AATAATGGTTGCATACAGGG+AGG 0.744657 1_3:+35489023 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGAATTAAAACTCAATTAA+TGG + chr1_3:35489185-35489204 None:intergenic 15.0%
!! ATTTATAGTTTCTTTCTTTA+TGG - chr1_3:35489695-35489714 Msa0116490:intron 15.0%
!! TTGAATTAAAACTCAATTAA+TGG + chr1_3:35489185-35489204 None:intergenic 15.0%
!! ATTTATAGTTTCTTTCTTTA+TGG - chr1_3:35489695-35489714 Msa0116490:intron 15.0%
!! CTAAGAAATGATAATTAATG+AGG + chr1_3:35489525-35489544 None:intergenic 20.0%
!! TTAACTCTCTTTGTATATTT+AGG - chr1_3:35489560-35489579 Msa0116490:CDS 20.0%
!! TAACTCTCTTTGTATATTTA+GGG - chr1_3:35489561-35489580 Msa0116490:CDS 20.0%
!! TCATTATAGCCATAAAATAA+TGG + chr1_3:35489917-35489936 None:intergenic 20.0%
!! AAAGATCATAATTCATAACA+AGG + chr1_3:35490033-35490052 None:intergenic 20.0%
!!! TCTTTTTTAGATATGTATAG+AGG + chr1_3:35490064-35490083 None:intergenic 20.0%
!! CTAAGAAATGATAATTAATG+AGG + chr1_3:35489525-35489544 None:intergenic 20.0%
!! TTAACTCTCTTTGTATATTT+AGG - chr1_3:35489560-35489579 Msa0116490:CDS 20.0%
!! TAACTCTCTTTGTATATTTA+GGG - chr1_3:35489561-35489580 Msa0116490:CDS 20.0%
!! TCATTATAGCCATAAAATAA+TGG + chr1_3:35489917-35489936 None:intergenic 20.0%
!! AAAGATCATAATTCATAACA+AGG + chr1_3:35490033-35490052 None:intergenic 20.0%
!!! TCTTTTTTAGATATGTATAG+AGG + chr1_3:35490064-35490083 None:intergenic 20.0%
! AACTTTCGATCATAAAACAA+AGG + chr1_3:35488855-35488874 None:intergenic 25.0%
! TCATACAAGTCATTTGTAAT+TGG - chr1_3:35489054-35489073 Msa0116490:exon 25.0%
!!! TCACAATAGTACTTTTTCAT+TGG + chr1_3:35489304-35489323 None:intergenic 25.0%
! ATCAGTAAATGAACAACTAT+CGG + chr1_3:35489677-35489696 None:intergenic 25.0%
! TATGTACTATGCATATATGT+AGG - chr1_3:35489745-35489764 Msa0116490:intron 25.0%
! ATGTACTATGCATATATGTA+GGG - chr1_3:35489746-35489765 Msa0116490:intron 25.0%
! ATATATGTAGGGAATATAGT+AGG - chr1_3:35489757-35489776 Msa0116490:intron 25.0%
! TAAAATAATGGTTGCATACA+GGG + chr1_3:35489905-35489924 None:intergenic 25.0%
! ATAAAATAATGGTTGCATAC+AGG + chr1_3:35489906-35489925 None:intergenic 25.0%
!!! TGTATGCAACCATTATTTTA+TGG - chr1_3:35489905-35489924 Msa0116490:five_prime_UTR 25.0%
!!! AACATACTAATAGTTCTGAT+AGG - chr1_3:35489937-35489956 Msa0116490:five_prime_UTR 25.0%
! ATAACAAGGTAACATTTAAC+AGG + chr1_3:35490019-35490038 None:intergenic 25.0%
! AACTTTCGATCATAAAACAA+AGG + chr1_3:35488855-35488874 None:intergenic 25.0%
! TCATACAAGTCATTTGTAAT+TGG - chr1_3:35489054-35489073 Msa0116490:exon 25.0%
!!! TCACAATAGTACTTTTTCAT+TGG + chr1_3:35489304-35489323 None:intergenic 25.0%
! ATCAGTAAATGAACAACTAT+CGG + chr1_3:35489677-35489696 None:intergenic 25.0%
! TATGTACTATGCATATATGT+AGG - chr1_3:35489745-35489764 Msa0116490:intron 25.0%
! ATGTACTATGCATATATGTA+GGG - chr1_3:35489746-35489765 Msa0116490:intron 25.0%
! ATATATGTAGGGAATATAGT+AGG - chr1_3:35489757-35489776 Msa0116490:intron 25.0%
! TAAAATAATGGTTGCATACA+GGG + chr1_3:35489905-35489924 None:intergenic 25.0%
! ATAAAATAATGGTTGCATAC+AGG + chr1_3:35489906-35489925 None:intergenic 25.0%
!!! TGTATGCAACCATTATTTTA+TGG - chr1_3:35489905-35489924 Msa0116490:five_prime_UTR 25.0%
!!! AACATACTAATAGTTCTGAT+AGG - chr1_3:35489937-35489956 Msa0116490:five_prime_UTR 25.0%
! ATAACAAGGTAACATTTAAC+AGG + chr1_3:35490019-35490038 None:intergenic 25.0%
TACAAGTCATTTGTAATTGG+CGG - chr1_3:35489057-35489076 Msa0116490:exon 30.0%
ATGAAACAAGAGCAAAGATA+AGG + chr1_3:35489128-35489147 None:intergenic 30.0%
! ATTTAGGGATTTTGTCGTTA+TGG - chr1_3:35489576-35489595 Msa0116490:CDS 30.0%
TCAGTAAATGAACAACTATC+GGG + chr1_3:35489676-35489695 None:intergenic 30.0%
AATATAGTAGGAGATACCAT+GGG - chr1_3:35489769-35489788 Msa0116490:intron 30.0%
!!! CTGATTTTGAGTTTCTTTAC+TGG - chr1_3:35489966-35489985 Msa0116490:five_prime_UTR 30.0%
! TACTGGTTATTACAACTACT+AGG - chr1_3:35489983-35490002 Msa0116490:five_prime_UTR 30.0%
TACAAGTCATTTGTAATTGG+CGG - chr1_3:35489057-35489076 Msa0116490:exon 30.0%
ATGAAACAAGAGCAAAGATA+AGG + chr1_3:35489128-35489147 None:intergenic 30.0%
! ATTTAGGGATTTTGTCGTTA+TGG - chr1_3:35489576-35489595 Msa0116490:CDS 30.0%
TCAGTAAATGAACAACTATC+GGG + chr1_3:35489676-35489695 None:intergenic 30.0%
AATATAGTAGGAGATACCAT+GGG - chr1_3:35489769-35489788 Msa0116490:intron 30.0%
!!! CTGATTTTGAGTTTCTTTAC+TGG - chr1_3:35489966-35489985 Msa0116490:five_prime_UTR 30.0%
! TACTGGTTATTACAACTACT+AGG - chr1_3:35489983-35490002 Msa0116490:five_prime_UTR 30.0%
ATGTGAGCGTAGAAAGAAAT+TGG + chr1_3:35489026-35489045 None:intergenic 35.0%
TTCTTTCTACGCTCACATTA+AGG - chr1_3:35489028-35489047 Msa0116490:three_prime_UTR 35.0%
AGCAAAGATAAGGATTGTTC+GGG + chr1_3:35489118-35489137 None:intergenic 35.0%
GAGCAAAGATAAGGATTGTT+CGG + chr1_3:35489119-35489138 None:intergenic 35.0%
! CTCTTGTTTCATGTTCACAT+GGG - chr1_3:35489136-35489155 Msa0116490:CDS 35.0%
CCTCAACTCTACTACAAATA+TGG + chr1_3:35489254-35489273 None:intergenic 35.0%
CCATATTTGTAGTAGAGTTG+AGG - chr1_3:35489251-35489270 Msa0116490:intron 35.0%
CAGTAAATGAACAACTATCG+GGG + chr1_3:35489675-35489694 None:intergenic 35.0%
GAATATAGTAGGAGATACCA+TGG - chr1_3:35489768-35489787 Msa0116490:intron 35.0%
AGATTTGTGCACATTACCTT+TGG - chr1_3:35489875-35489894 Msa0116490:intron 35.0%
GATTTGTGCACATTACCTTT+GGG - chr1_3:35489876-35489895 Msa0116490:intron 35.0%
AGATATGTATAGAGGAGAGA+TGG + chr1_3:35490056-35490075 None:intergenic 35.0%
ATGTGAGCGTAGAAAGAAAT+TGG + chr1_3:35489026-35489045 None:intergenic 35.0%
TTCTTTCTACGCTCACATTA+AGG - chr1_3:35489028-35489047 Msa0116490:three_prime_UTR 35.0%
AGCAAAGATAAGGATTGTTC+GGG + chr1_3:35489118-35489137 None:intergenic 35.0%
GAGCAAAGATAAGGATTGTT+CGG + chr1_3:35489119-35489138 None:intergenic 35.0%
! CTCTTGTTTCATGTTCACAT+GGG - chr1_3:35489136-35489155 Msa0116490:CDS 35.0%
CCTCAACTCTACTACAAATA+TGG + chr1_3:35489254-35489273 None:intergenic 35.0%
CCATATTTGTAGTAGAGTTG+AGG - chr1_3:35489251-35489270 Msa0116490:intron 35.0%
CAGTAAATGAACAACTATCG+GGG + chr1_3:35489675-35489694 None:intergenic 35.0%
GAATATAGTAGGAGATACCA+TGG - chr1_3:35489768-35489787 Msa0116490:intron 35.0%
AGATTTGTGCACATTACCTT+TGG - chr1_3:35489875-35489894 Msa0116490:intron 35.0%
GATTTGTGCACATTACCTTT+GGG - chr1_3:35489876-35489895 Msa0116490:intron 35.0%
AGATATGTATAGAGGAGAGA+TGG + chr1_3:35490056-35490075 None:intergenic 35.0%
! GCTCTTGTTTCATGTTCACA+TGG - chr1_3:35489135-35489154 Msa0116490:CDS 40.0%
TTCAAGATACACCCTTAGAC+CGG - chr1_3:35489341-35489360 Msa0116490:CDS 40.0%
! GGCTTGTTTGTGTTGAATAC+CGG + chr1_3:35489380-35489399 None:intergenic 40.0%
! TGGAATAGAGTTCTGGTGTT+CGG + chr1_3:35489431-35489450 None:intergenic 40.0%
CAACCAATAACAAGAATGCC+AGG - chr1_3:35489642-35489661 Msa0116490:exon 40.0%
! TCACCTGGCATTCTTGTTAT+TGG + chr1_3:35489648-35489667 None:intergenic 40.0%
AATAATGGTTGCATACAGGG+AGG + chr1_3:35489902-35489921 None:intergenic 40.0%
! GCTCTTGTTTCATGTTCACA+TGG - chr1_3:35489135-35489154 Msa0116490:CDS 40.0%
TTCAAGATACACCCTTAGAC+CGG - chr1_3:35489341-35489360 Msa0116490:CDS 40.0%
! GGCTTGTTTGTGTTGAATAC+CGG + chr1_3:35489380-35489399 None:intergenic 40.0%
! TGGAATAGAGTTCTGGTGTT+CGG + chr1_3:35489431-35489450 None:intergenic 40.0%
CAACCAATAACAAGAATGCC+AGG - chr1_3:35489642-35489661 Msa0116490:exon 40.0%
! TCACCTGGCATTCTTGTTAT+TGG + chr1_3:35489648-35489667 None:intergenic 40.0%
AATAATGGTTGCATACAGGG+AGG + chr1_3:35489902-35489921 None:intergenic 40.0%
GGCAGGCATACAAGGAAAAT+GGG + chr1_3:35488882-35488901 None:intergenic 45.0%
AGGCAGGCATACAAGGAAAA+TGG + chr1_3:35488883-35488902 None:intergenic 45.0%
TTGCAACTTCAAGCTTCGTG+CGG + chr1_3:35489088-35489107 None:intergenic 45.0%
TTTGTAGTAGAGTTGAGGCG+TGG - chr1_3:35489256-35489275 Msa0116490:intron 45.0%
TTGTAGTAGAGTTGAGGCGT+GGG - chr1_3:35489257-35489276 Msa0116490:intron 45.0%
ACAAACAAGCCAAAGCTCGT+TGG - chr1_3:35489389-35489408 Msa0116490:intron 45.0%
! GGAATAGAGTTCTGGTGTTC+GGG + chr1_3:35489430-35489449 None:intergenic 45.0%
!! ATGGTGGTGGAATAGAGTTC+TGG + chr1_3:35489438-35489457 None:intergenic 45.0%
! AAGGGTTGATTAGATGGTGG+TGG + chr1_3:35489451-35489470 None:intergenic 45.0%
! CAGAAGGGTTGATTAGATGG+TGG + chr1_3:35489454-35489473 None:intergenic 45.0%
! ACGCAGAAGGGTTGATTAGA+TGG + chr1_3:35489457-35489476 None:intergenic 45.0%
! ATAGTACCGTGTTGACGAAG+TGG + chr1_3:35489480-35489499 None:intergenic 45.0%
TGCTGCTGCTGTTGTGTATT+GGG + chr1_3:35489624-35489643 None:intergenic 45.0%
TTGCTGCTGCTGTTGTGTAT+TGG + chr1_3:35489625-35489644 None:intergenic 45.0%
TCTATCCCCAGTCGACAAAT+GGG + chr1_3:35489858-35489877 None:intergenic 45.0%
ATCTATCCCCAGTCGACAAA+TGG + chr1_3:35489859-35489878 None:intergenic 45.0%
GGCAGGCATACAAGGAAAAT+GGG + chr1_3:35488882-35488901 None:intergenic 45.0%
AGGCAGGCATACAAGGAAAA+TGG + chr1_3:35488883-35488902 None:intergenic 45.0%
TTGCAACTTCAAGCTTCGTG+CGG + chr1_3:35489088-35489107 None:intergenic 45.0%
TTTGTAGTAGAGTTGAGGCG+TGG - chr1_3:35489256-35489275 Msa0116490:intron 45.0%
TTGTAGTAGAGTTGAGGCGT+GGG - chr1_3:35489257-35489276 Msa0116490:intron 45.0%
ACAAACAAGCCAAAGCTCGT+TGG - chr1_3:35489389-35489408 Msa0116490:intron 45.0%
! GGAATAGAGTTCTGGTGTTC+GGG + chr1_3:35489430-35489449 None:intergenic 45.0%
!! ATGGTGGTGGAATAGAGTTC+TGG + chr1_3:35489438-35489457 None:intergenic 45.0%
! AAGGGTTGATTAGATGGTGG+TGG + chr1_3:35489451-35489470 None:intergenic 45.0%
! CAGAAGGGTTGATTAGATGG+TGG + chr1_3:35489454-35489473 None:intergenic 45.0%
! ACGCAGAAGGGTTGATTAGA+TGG + chr1_3:35489457-35489476 None:intergenic 45.0%
! ATAGTACCGTGTTGACGAAG+TGG + chr1_3:35489480-35489499 None:intergenic 45.0%
TGCTGCTGCTGTTGTGTATT+GGG + chr1_3:35489624-35489643 None:intergenic 45.0%
TTGCTGCTGCTGTTGTGTAT+TGG + chr1_3:35489625-35489644 None:intergenic 45.0%
TCTATCCCCAGTCGACAAAT+GGG + chr1_3:35489858-35489877 None:intergenic 45.0%
ATCTATCCCCAGTCGACAAA+TGG + chr1_3:35489859-35489878 None:intergenic 45.0%
AGACACAAGGCAGGCATACA+AGG + chr1_3:35488890-35488909 None:intergenic 50.0%
AGTCCGACAACCTCTGTTCT+AGG - chr1_3:35488911-35488930 Msa0116490:three_prime_UTR 50.0%
GGACCTAGAACAGAGGTTGT+CGG + chr1_3:35488917-35488936 None:intergenic 50.0%
! GACAACCTCTGTTCTAGGTC+CGG - chr1_3:35488916-35488935 Msa0116490:three_prime_UTR 50.0%
AATGGAAGTGGCAGGCAGAA+CGG + chr1_3:35488967-35488986 None:intergenic 50.0%
TCGCATAGAATGGAAGTGGC+AGG + chr1_3:35488975-35488994 None:intergenic 50.0%
TGTGTTGAATACCGGCAGAG+TGG + chr1_3:35489372-35489391 None:intergenic 50.0%
GGAATCGTACCAACGAGCTT+TGG + chr1_3:35489401-35489420 None:intergenic 50.0%
! GTTCTGGTGTTCGGGTTTGA+AGG + chr1_3:35489422-35489441 None:intergenic 50.0%
TTGACGAAGTGGACGCAGAA+GGG + chr1_3:35489469-35489488 None:intergenic 50.0%
!! GATACCATGGGAGTGTCGTT+CGG - chr1_3:35489781-35489800 Msa0116490:intron 50.0%
! CAACCTCCCATTTGTCGACT+GGG - chr1_3:35489849-35489868 Msa0116490:intron 50.0%
! AACCTCCCATTTGTCGACTG+GGG - chr1_3:35489850-35489869 Msa0116490:intron 50.0%
AGACACAAGGCAGGCATACA+AGG + chr1_3:35488890-35488909 None:intergenic 50.0%
AGTCCGACAACCTCTGTTCT+AGG - chr1_3:35488911-35488930 Msa0116490:three_prime_UTR 50.0%
GGACCTAGAACAGAGGTTGT+CGG + chr1_3:35488917-35488936 None:intergenic 50.0%
! GACAACCTCTGTTCTAGGTC+CGG - chr1_3:35488916-35488935 Msa0116490:three_prime_UTR 50.0%
AATGGAAGTGGCAGGCAGAA+CGG + chr1_3:35488967-35488986 None:intergenic 50.0%
TCGCATAGAATGGAAGTGGC+AGG + chr1_3:35488975-35488994 None:intergenic 50.0%
TGTGTTGAATACCGGCAGAG+TGG + chr1_3:35489372-35489391 None:intergenic 50.0%
GGAATCGTACCAACGAGCTT+TGG + chr1_3:35489401-35489420 None:intergenic 50.0%
! GTTCTGGTGTTCGGGTTTGA+AGG + chr1_3:35489422-35489441 None:intergenic 50.0%
TTGACGAAGTGGACGCAGAA+GGG + chr1_3:35489469-35489488 None:intergenic 50.0%
!! GATACCATGGGAGTGTCGTT+CGG - chr1_3:35489781-35489800 Msa0116490:intron 50.0%
! CAACCTCCCATTTGTCGACT+GGG - chr1_3:35489849-35489868 Msa0116490:intron 50.0%
! AACCTCCCATTTGTCGACTG+GGG - chr1_3:35489850-35489869 Msa0116490:intron 50.0%
GGTTGTCGGACTCAGACACA+AGG + chr1_3:35488903-35488922 None:intergenic 55.0%
GGCGTCGCATAGAATGGAAG+TGG + chr1_3:35488979-35488998 None:intergenic 55.0%
!! AACGGAGGCGTCGCATAGAA+TGG + chr1_3:35488985-35489004 None:intergenic 55.0%
CAACTTCAAGCTTCGTGCGG+TGG + chr1_3:35489085-35489104 None:intergenic 55.0%
GAGTGGCGTTTCCGGTCTAA+GGG + chr1_3:35489355-35489374 None:intergenic 55.0%
AGAGTGGCGTTTCCGGTCTA+AGG + chr1_3:35489356-35489375 None:intergenic 55.0%
GTTGACGAAGTGGACGCAGA+AGG + chr1_3:35489470-35489489 None:intergenic 55.0%
CTGCGTCCACTTCGTCAACA+CGG - chr1_3:35489471-35489490 Msa0116490:CDS 55.0%
AACTATCGGGGTAGCTCACC+TGG + chr1_3:35489663-35489682 None:intergenic 55.0%
ATTGCCGAACGACACTCCCA+TGG + chr1_3:35489788-35489807 None:intergenic 55.0%
! TTGGAGTATCCACCCTCAGG+CGG + chr1_3:35489832-35489851 None:intergenic 55.0%
! AGGTTGGAGTATCCACCCTC+AGG + chr1_3:35489835-35489854 None:intergenic 55.0%
CCAGTCGACAAATGGGAGGT+TGG + chr1_3:35489851-35489870 None:intergenic 55.0%
CCAACCTCCCATTTGTCGAC+TGG - chr1_3:35489848-35489867 Msa0116490:intron 55.0%
ATCCCCAGTCGACAAATGGG+AGG + chr1_3:35489855-35489874 None:intergenic 55.0%
TTGCATACAGGGAGGCCCAA+AGG + chr1_3:35489894-35489913 None:intergenic 55.0%
GGTTGTCGGACTCAGACACA+AGG + chr1_3:35488903-35488922 None:intergenic 55.0%
GGCGTCGCATAGAATGGAAG+TGG + chr1_3:35488979-35488998 None:intergenic 55.0%
!! AACGGAGGCGTCGCATAGAA+TGG + chr1_3:35488985-35489004 None:intergenic 55.0%
CAACTTCAAGCTTCGTGCGG+TGG + chr1_3:35489085-35489104 None:intergenic 55.0%
GAGTGGCGTTTCCGGTCTAA+GGG + chr1_3:35489355-35489374 None:intergenic 55.0%
AGAGTGGCGTTTCCGGTCTA+AGG + chr1_3:35489356-35489375 None:intergenic 55.0%
GTTGACGAAGTGGACGCAGA+AGG + chr1_3:35489470-35489489 None:intergenic 55.0%
CTGCGTCCACTTCGTCAACA+CGG - chr1_3:35489471-35489490 Msa0116490:CDS 55.0%
AACTATCGGGGTAGCTCACC+TGG + chr1_3:35489663-35489682 None:intergenic 55.0%
ATTGCCGAACGACACTCCCA+TGG + chr1_3:35489788-35489807 None:intergenic 55.0%
! TTGGAGTATCCACCCTCAGG+CGG + chr1_3:35489832-35489851 None:intergenic 55.0%
! AGGTTGGAGTATCCACCCTC+AGG + chr1_3:35489835-35489854 None:intergenic 55.0%
CCAGTCGACAAATGGGAGGT+TGG + chr1_3:35489851-35489870 None:intergenic 55.0%
CCAACCTCCCATTTGTCGAC+TGG - chr1_3:35489848-35489867 Msa0116490:intron 55.0%
ATCCCCAGTCGACAAATGGG+AGG + chr1_3:35489855-35489874 None:intergenic 55.0%
TTGCATACAGGGAGGCCCAA+AGG + chr1_3:35489894-35489913 None:intergenic 55.0%
GTCGGACTCAGACACAAGGC+AGG + chr1_3:35488899-35488918 None:intergenic 60.0%
TACAGCGTCGTCGCGCAGAA+CGG + chr1_3:35489003-35489022 None:intergenic 60.0%
GTAGAGTTGAGGCGTGGGTG+TGG - chr1_3:35489262-35489281 Msa0116490:intron 60.0%
TACCGGCAGAGTGGCGTTTC+CGG + chr1_3:35489363-35489382 None:intergenic 60.0%
GTCGGACTCAGACACAAGGC+AGG + chr1_3:35488899-35488918 None:intergenic 60.0%
TACAGCGTCGTCGCGCAGAA+CGG + chr1_3:35489003-35489022 None:intergenic 60.0%
GTAGAGTTGAGGCGTGGGTG+TGG - chr1_3:35489262-35489281 Msa0116490:intron 60.0%
TACCGGCAGAGTGGCGTTTC+CGG + chr1_3:35489363-35489382 None:intergenic 60.0%
TCGCGCCGGACCTAGAACAG+AGG + chr1_3:35488924-35488943 None:intergenic 65.0%
AACGGCGGCGTCCTGGAGAA+CGG + chr1_3:35488949-35488968 None:intergenic 65.0%
GGAAGTGGCAGGCAGAACGG+CGG + chr1_3:35488964-35488983 None:intergenic 65.0%
GTTGAGGCGTGGGTGTGGTG+AGG - chr1_3:35489267-35489286 Msa0116490:intron 65.0%
AGGCGTGGGTGTGGTGAGGA+GGG - chr1_3:35489271-35489290 Msa0116490:intron 65.0%
GACCGGAAACGCCACTCTGC+CGG - chr1_3:35489358-35489377 Msa0116490:intron 65.0%
TCAGGCGGAGGCTGCAGTGA+CGG + chr1_3:35489817-35489836 None:intergenic 65.0%
TCACTGCAGCCTCCGCCTGA+GGG - chr1_3:35489817-35489836 Msa0116490:intron 65.0%
GAGTATCCACCCTCAGGCGG+AGG + chr1_3:35489829-35489848 None:intergenic 65.0%
TCGCGCCGGACCTAGAACAG+AGG + chr1_3:35488924-35488943 None:intergenic 65.0%
AACGGCGGCGTCCTGGAGAA+CGG + chr1_3:35488949-35488968 None:intergenic 65.0%
GGAAGTGGCAGGCAGAACGG+CGG + chr1_3:35488964-35488983 None:intergenic 65.0%
GTTGAGGCGTGGGTGTGGTG+AGG - chr1_3:35489267-35489286 Msa0116490:intron 65.0%
AGGCGTGGGTGTGGTGAGGA+GGG - chr1_3:35489271-35489290 Msa0116490:intron 65.0%
GACCGGAAACGCCACTCTGC+CGG - chr1_3:35489358-35489377 Msa0116490:intron 65.0%
TCAGGCGGAGGCTGCAGTGA+CGG + chr1_3:35489817-35489836 None:intergenic 65.0%
TCACTGCAGCCTCCGCCTGA+GGG - chr1_3:35489817-35489836 Msa0116490:intron 65.0%
GAGTATCCACCCTCAGGCGG+AGG + chr1_3:35489829-35489848 None:intergenic 65.0%
!! CCTGGAGAACGGTGTCGCGC+CGG + chr1_3:35488938-35488957 None:intergenic 70.0%
AGCGTCGTCGCGCAGAACGG+AGG + chr1_3:35489000-35489019 None:intergenic 70.0%
GAGGCGTGGGTGTGGTGAGG+AGG - chr1_3:35489270-35489289 Msa0116490:intron 70.0%
GGCGTGGGTGTGGTGAGGAG+GGG - chr1_3:35489272-35489291 Msa0116490:intron 70.0%
GTCACTGCAGCCTCCGCCTG+AGG - chr1_3:35489816-35489835 Msa0116490:intron 70.0%
!! CCTGGAGAACGGTGTCGCGC+CGG + chr1_3:35488938-35488957 None:intergenic 70.0%
AGCGTCGTCGCGCAGAACGG+AGG + chr1_3:35489000-35489019 None:intergenic 70.0%
GAGGCGTGGGTGTGGTGAGG+AGG - chr1_3:35489270-35489289 Msa0116490:intron 70.0%
GGCGTGGGTGTGGTGAGGAG+GGG - chr1_3:35489272-35489291 Msa0116490:intron 70.0%
GTCACTGCAGCCTCCGCCTG+AGG - chr1_3:35489816-35489835 Msa0116490:intron 70.0%
CCGGCGCGACACCGTTCTCC+AGG - chr1_3:35488935-35488954 Msa0116490:three_prime_UTR 75.0%
CAGGCAGAACGGCGGCGTCC+TGG + chr1_3:35488956-35488975 None:intergenic 75.0%
GGCGGAGGCTGCAGTGACGG+AGG + chr1_3:35489814-35489833 None:intergenic 75.0%
CTGCAGCCTCCGCCTGAGGG+TGG - chr1_3:35489820-35489839 Msa0116490:intron 75.0%
CCGGCGCGACACCGTTCTCC+AGG - chr1_3:35488935-35488954 Msa0116490:three_prime_UTR 75.0%
CAGGCAGAACGGCGGCGTCC+TGG + chr1_3:35488956-35488975 None:intergenic 75.0%
GGCGGAGGCTGCAGTGACGG+AGG + chr1_3:35489814-35489833 None:intergenic 75.0%
CTGCAGCCTCCGCCTGAGGG+TGG - chr1_3:35489820-35489839 Msa0116490:intron 75.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 35488848 35490096 35488848 ID=Msa0116490;Name=Msa0116490
chr1_3 mRNA 35488848 35490096 35488848 ID=Msa0116490-mRNA-1;Parent=Msa0116490;Name=Msa0116490-mRNA-1;_AED=0.01;_eAED=0.01;_QI=266|1|1|1|1|1|4|220|128
chr1_3 exon 35488848 35489177 35488848 ID=Msa0116490-mRNA-1:exon:11398;Parent=Msa0116490-mRNA-1
chr1_3 exon 35489281 35489362 35489281 ID=Msa0116490-mRNA-1:exon:11397;Parent=Msa0116490-mRNA-1
chr1_3 exon 35489452 35489710 35489452 ID=Msa0116490-mRNA-1:exon:11396;Parent=Msa0116490-mRNA-1
chr1_3 exon 35489895 35490096 35489895 ID=Msa0116490-mRNA-1:exon:11395;Parent=Msa0116490-mRNA-1
chr1_3 five_prime_UTR 35489895 35490096 35489895 ID=Msa0116490-mRNA-1:five_prime_utr;Parent=Msa0116490-mRNA-1
chr1_3 five_prime_UTR 35489647 35489710 35489647 ID=Msa0116490-mRNA-1:five_prime_utr;Parent=Msa0116490-mRNA-1
chr1_3 CDS 35489452 35489646 35489452 ID=Msa0116490-mRNA-1:cds;Parent=Msa0116490-mRNA-1
chr1_3 CDS 35489281 35489362 35489281 ID=Msa0116490-mRNA-1:cds;Parent=Msa0116490-mRNA-1
chr1_3 CDS 35489068 35489177 35489068 ID=Msa0116490-mRNA-1:cds;Parent=Msa0116490-mRNA-1
chr1_3 three_prime_UTR 35488848 35489067 35488848 ID=Msa0116490-mRNA-1:three_prime_utr;Parent=Msa0116490-mRNA-1
Gene Sequence

>Msa0116490

ATGCCAATGAAAAAGTACTATTGTGAGTATTGCGATAAGCAATTTCAAGATACACCCTTAGACCGGAAACGCCACTCTGCCGGTATTCAACACAAACAAGCCAAAGCTCGTTGGTACGATTCCTTCAAACCCGAACACCAGAACTCTATTCCACCACCATCTAATCAACCCTTCTGCGTCCACTTCGTCAACACGGGATTTTGTCGTTATGGCGATTCTTGCAAATATTTTCATCCCAATACACAACAGCAGCAGCAACCAATAACAAGAATGCCAGGGAATATAGTAGGAGATACCATGGGAGTGTCGTTCGGCAATTTGCCTCCGTCACTGCAGCCTCCGCCTGAGGGTGGATACTCCAACCTCCCATTTGTCGACTGGGGATAG
Protein sequence

>Msa0116490

MPMKKYYCEYCDKQFQDTPLDRKRHSAGIQHKQAKARWYDSFKPEHQNSIPPPSNQPFCVHFVNTGFCRYGDSCKYFHPNTQQQQQPITRMPGNIVGDTMGVSFGNLPPSLQPPPEGGYSNLPFVDWG*