AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0124400


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0124400 MtrunA17_Chr1g0185331 94.561 239 11 1 1 239 1 237 9.03e-169 464
Msa0124400 MtrunA17_Chr7g0260011 56.757 259 89 8 1 238 1 257 7.76e-83 248
Msa0124400 MtrunA17_Chr1g0205211 53.704 108 36 2 143 237 180 286 7.88e-30 112
Msa0124400 MtrunA17_Chr1g0205211 44.444 117 49 7 30 138 12 120 9.07e-15 72.0
Msa0124400 MtrunA17_Chr3g0138651 47.826 115 36 3 146 236 280 394 6.91e-24 99.0
Msa0124400 MtrunA17_Chr1g0148411 48.214 112 37 3 146 236 204 315 1.91e-23 96.7
Msa0124400 MtrunA17_Chr2g0331941 47.321 112 48 4 127 228 240 350 2.59e-20 88.6
Msa0124400 MtrunA17_Chr2g0301141 59.091 44 17 1 193 236 183 225 9.29e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0124400 AT3G63210.1 40.449 267 124 11 2 238 1 262 1.64e-48 160
Msa0124400 AT3G22550.1 37.319 276 118 10 2 236 1 262 1.99e-43 147
Msa0124400 AT5G11460.1 27.341 267 105 8 52 232 44 307 7.81e-22 92.8
Msa0124400 AT2G25690.2 44.330 97 35 3 155 238 218 308 3.86e-17 79.3
Msa0124400 AT2G25690.1 44.330 97 35 3 155 238 218 308 3.86e-17 79.3
Msa0124400 AT5G20700.1 32.227 211 93 11 54 236 33 221 9.71e-12 63.5

Find 35 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTTCACTTGAGGAGTTTA+AGG 0.190065 1_3:-52180174 None:intergenic
ACTACAATACCATCTCTATT+TGG 0.228214 1_3:+52180328 Msa0124400:CDS
CAAAAGACATCTTCATTTAC+AGG 0.305275 1_3:+52180881 Msa0124400:CDS
GCTACAAACTTTGTTCCTAT+TGG 0.308716 1_3:-52180479 None:intergenic
CACACAAATAGAACTTAAAA+AGG 0.313516 1_3:-52181114 None:intergenic
GGTCTTGGTCTTGGTCTTGT+TGG 0.321698 1_3:+52180532 Msa0124400:CDS
TTCACCCATCAAACTTTGTT+TGG 0.353951 1_3:-52180284 None:intergenic
AAAACCATTGGTCTTGGTCT+TGG 0.366469 1_3:+52180523 Msa0124400:CDS
AGCAATAGAAAAGTTCTGTT+TGG 0.378889 1_3:+52180601 Msa0124400:CDS
AAATGAAAATCTCTATAGTT+TGG 0.385441 1_3:-52180355 None:intergenic
ATATGACTGAATAATATATA+TGG 0.390307 1_3:-52179950 None:intergenic
TTCCTATTGGTAATTGAGAA+AGG 0.411279 1_3:-52180466 None:intergenic
ATCTCTATTTGCAAGGTTGA+AGG 0.413036 1_3:-52181146 None:intergenic
ACCCTTTCTCAATTACCAAT+AGG 0.433358 1_3:+52180464 Msa0124400:CDS
AATTCAGTACACTTTCACTT+AGG 0.454438 1_3:+52180142 Msa0124400:five_prime_UTR
TGTTCTTAGTTTGAGTGAGA+TGG 0.477236 1_3:+52180684 Msa0124400:CDS
AGTCACCAAACAAAGTTTGA+TGG 0.487954 1_3:+52180279 Msa0124400:CDS
TCCTATTGGTAATTGAGAAA+GGG 0.489680 1_3:-52180465 None:intergenic
ATTGAAAAGTGTTTCACTTG+AGG 0.491387 1_3:-52180183 None:intergenic
AAAGTGTACTGAATTGTGTT+TGG 0.502524 1_3:-52180134 None:intergenic
TCAAACATGGAAGGTGGTAG+AGG 0.507899 1_3:-52180649 None:intergenic
GGAACAAAGTTTGTAGCTCA+AGG 0.520520 1_3:+52180485 Msa0124400:CDS
TACACATGTGTGATATCTCA+TGG 0.544339 1_3:+52180721 Msa0124400:CDS
CTTGCAAATAGAGATAAAAG+AGG 0.555159 1_3:+52181154 Msa0124400:three_prime_UTR
GTCACCAAACAAAGTTTGAT+GGG 0.561348 1_3:+52180280 Msa0124400:CDS
ACATGGAAGGTGGTAGAGGA+GGG 0.569491 1_3:-52180645 None:intergenic
TTTGTGTCAAGAATTGATGT+TGG 0.576565 1_3:-52180421 None:intergenic
AACATGGAAGGTGGTAGAGG+AGG 0.584377 1_3:-52180646 None:intergenic
TCTATTTGCAAGGTTGAAGG+AGG 0.594942 1_3:-52181143 None:intergenic
TATATTTGATAATTGTGTTG+TGG 0.597437 1_3:+52180765 Msa0124400:CDS
GAACAAAGTTTGTAGCTCAA+GGG 0.606947 1_3:+52180486 Msa0124400:CDS
TTTGGTGAACCAAATAGAGA+TGG 0.621510 1_3:-52180337 None:intergenic
TGTGACTCAAACATGGAAGG+TGG 0.643194 1_3:-52180655 None:intergenic
AAGACCAAGACCAAGACCAA+TGG 0.659900 1_3:-52180527 None:intergenic
AGAATGTAGACACAAAGAGA+TGG 0.662022 1_3:+52181031 Msa0124400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATATATATGGCTTTTAA+AGG - chr1_3:52179943-52179962 None:intergenic 15.0%
!! ATATGACTGAATAATATATA+TGG - chr1_3:52179953-52179972 None:intergenic 15.0%
!!! TTTTTTTTTTTTTTATGCAG+AGG + chr1_3:52180987-52181006 Msa0124400:intron 15.0%
!!! ATAATATATATGGCTTTTAA+AGG - chr1_3:52179943-52179962 None:intergenic 15.0%
!! ATATGACTGAATAATATATA+TGG - chr1_3:52179953-52179972 None:intergenic 15.0%
!!! TTTTTTTTTTTTTTATGCAG+AGG + chr1_3:52180987-52181006 Msa0124400:intron 15.0%
!!! ATTGTAGTGAAATTTTGATT+TGG - chr1_3:52180316-52180335 None:intergenic 20.0%
!! AAATGAAAATCTCTATAGTT+TGG - chr1_3:52180358-52180377 None:intergenic 20.0%
!!! CAAATATATGAGTTGTTTTT+GGG - chr1_3:52180753-52180772 None:intergenic 20.0%
!!! TCAAATATATGAGTTGTTTT+TGG - chr1_3:52180754-52180773 None:intergenic 20.0%
!!! TATATTTGATAATTGTGTTG+TGG + chr1_3:52180765-52180784 Msa0124400:CDS 20.0%
!!! ATTGTAGTGAAATTTTGATT+TGG - chr1_3:52180316-52180335 None:intergenic 20.0%
!! AAATGAAAATCTCTATAGTT+TGG - chr1_3:52180358-52180377 None:intergenic 20.0%
!!! CAAATATATGAGTTGTTTTT+GGG - chr1_3:52180753-52180772 None:intergenic 20.0%
!!! TCAAATATATGAGTTGTTTT+TGG - chr1_3:52180754-52180773 None:intergenic 20.0%
!!! TATATTTGATAATTGTGTTG+TGG + chr1_3:52180765-52180784 Msa0124400:CDS 20.0%
! AAAGAAAGAGAAGAAAAAGA+AGG - chr1_3:52180002-52180021 None:intergenic 25.0%
! AAAGTAGATTCAAAAACCAT+TGG + chr1_3:52180511-52180530 Msa0124400:CDS 25.0%
!!! TGATCAGAATTTTGATGAAA+AGG - chr1_3:52180574-52180593 None:intergenic 25.0%
! CACACAAATAGAACTTAAAA+AGG - chr1_3:52181117-52181136 None:intergenic 25.0%
! AAAGAAAGAGAAGAAAAAGA+AGG - chr1_3:52180002-52180021 None:intergenic 25.0%
! AAAGTAGATTCAAAAACCAT+TGG + chr1_3:52180511-52180530 Msa0124400:CDS 25.0%
!!! TGATCAGAATTTTGATGAAA+AGG - chr1_3:52180574-52180593 None:intergenic 25.0%
! CACACAAATAGAACTTAAAA+AGG - chr1_3:52181117-52181136 None:intergenic 25.0%
! AAAGAAGGAAACTTTTGACA+TGG - chr1_3:52179987-52180006 None:intergenic 30.0%
AAAGTGTACTGAATTGTGTT+TGG - chr1_3:52180137-52180156 None:intergenic 30.0%
AATTCAGTACACTTTCACTT+AGG + chr1_3:52180142-52180161 Msa0124400:five_prime_UTR 30.0%
!! ATTGAAAAGTGTTTCACTTG+AGG - chr1_3:52180186-52180205 None:intergenic 30.0%
ACTACAATACCATCTCTATT+TGG + chr1_3:52180328-52180347 Msa0124400:CDS 30.0%
TTTGTGTCAAGAATTGATGT+TGG - chr1_3:52180424-52180443 None:intergenic 30.0%
! TCCTATTGGTAATTGAGAAA+GGG - chr1_3:52180468-52180487 None:intergenic 30.0%
! TTCCTATTGGTAATTGAGAA+AGG - chr1_3:52180469-52180488 None:intergenic 30.0%
AGCAATAGAAAAGTTCTGTT+TGG + chr1_3:52180601-52180620 Msa0124400:CDS 30.0%
!!! ACTTTTTTGTGACTCAAACA+TGG - chr1_3:52180665-52180684 None:intergenic 30.0%
!!! ATATGAGTTGTTTTTGGGTT+TGG - chr1_3:52180748-52180767 None:intergenic 30.0%
AAAAAGAGTGAGGACTATTA+GGG - chr1_3:52180810-52180829 None:intergenic 30.0%
AAATTCAGTGAAAAAGAGTG+AGG - chr1_3:52180820-52180839 None:intergenic 30.0%
CAAAAGACATCTTCATTTAC+AGG + chr1_3:52180881-52180900 Msa0124400:CDS 30.0%
TACTTGAGCAAAGCTTATTA+AGG + chr1_3:52180947-52180966 Msa0124400:intron 30.0%
!! ATTAAGGAGCATTAACTTGA+TGG + chr1_3:52180963-52180982 Msa0124400:intron 30.0%
! CTCTTTTATCTCTATTTGCA+AGG - chr1_3:52181156-52181175 None:intergenic 30.0%
CTTGCAAATAGAGATAAAAG+AGG + chr1_3:52181154-52181173 Msa0124400:three_prime_UTR 30.0%
! AAAGAAGGAAACTTTTGACA+TGG - chr1_3:52179987-52180006 None:intergenic 30.0%
AAAGTGTACTGAATTGTGTT+TGG - chr1_3:52180137-52180156 None:intergenic 30.0%
AATTCAGTACACTTTCACTT+AGG + chr1_3:52180142-52180161 Msa0124400:five_prime_UTR 30.0%
!! ATTGAAAAGTGTTTCACTTG+AGG - chr1_3:52180186-52180205 None:intergenic 30.0%
ACTACAATACCATCTCTATT+TGG + chr1_3:52180328-52180347 Msa0124400:CDS 30.0%
TTTGTGTCAAGAATTGATGT+TGG - chr1_3:52180424-52180443 None:intergenic 30.0%
! TCCTATTGGTAATTGAGAAA+GGG - chr1_3:52180468-52180487 None:intergenic 30.0%
! TTCCTATTGGTAATTGAGAA+AGG - chr1_3:52180469-52180488 None:intergenic 30.0%
AGCAATAGAAAAGTTCTGTT+TGG + chr1_3:52180601-52180620 Msa0124400:CDS 30.0%
!!! ACTTTTTTGTGACTCAAACA+TGG - chr1_3:52180665-52180684 None:intergenic 30.0%
!!! ATATGAGTTGTTTTTGGGTT+TGG - chr1_3:52180748-52180767 None:intergenic 30.0%
AAAAAGAGTGAGGACTATTA+GGG - chr1_3:52180810-52180829 None:intergenic 30.0%
AAATTCAGTGAAAAAGAGTG+AGG - chr1_3:52180820-52180839 None:intergenic 30.0%
CAAAAGACATCTTCATTTAC+AGG + chr1_3:52180881-52180900 Msa0124400:CDS 30.0%
TACTTGAGCAAAGCTTATTA+AGG + chr1_3:52180947-52180966 Msa0124400:intron 30.0%
!! ATTAAGGAGCATTAACTTGA+TGG + chr1_3:52180963-52180982 Msa0124400:intron 30.0%
! CTCTTTTATCTCTATTTGCA+AGG - chr1_3:52181156-52181175 None:intergenic 30.0%
CTTGCAAATAGAGATAAAAG+AGG + chr1_3:52181154-52181173 Msa0124400:three_prime_UTR 30.0%
! GAAAAAGCATGATTTAGCAG+TGG - chr1_3:52180092-52180111 None:intergenic 35.0%
TGTTTCACTTGAGGAGTTTA+AGG - chr1_3:52180177-52180196 None:intergenic 35.0%
AGTCACCAAACAAAGTTTGA+TGG + chr1_3:52180279-52180298 Msa0124400:CDS 35.0%
GTCACCAAACAAAGTTTGAT+GGG + chr1_3:52180280-52180299 Msa0124400:CDS 35.0%
TTCACCCATCAAACTTTGTT+TGG - chr1_3:52180287-52180306 None:intergenic 35.0%
TTTGGTGAACCAAATAGAGA+TGG - chr1_3:52180340-52180359 None:intergenic 35.0%
ACTTCCAAAAAATCTCTCAC+TGG + chr1_3:52180379-52180398 Msa0124400:CDS 35.0%
!!! AGTTCCAGTGAGAGATTTTT+TGG - chr1_3:52180386-52180405 None:intergenic 35.0%
! TGAGAAAGGGTTTTCAAATG+AGG - chr1_3:52180455-52180474 None:intergenic 35.0%
ACCCTTTCTCAATTACCAAT+AGG + chr1_3:52180464-52180483 Msa0124400:CDS 35.0%
GCTACAAACTTTGTTCCTAT+TGG - chr1_3:52180482-52180501 None:intergenic 35.0%
GAACAAAGTTTGTAGCTCAA+GGG + chr1_3:52180486-52180505 Msa0124400:CDS 35.0%
! GATTCAAAAACCATTGGTCT+TGG + chr1_3:52180517-52180536 Msa0124400:CDS 35.0%
! TTTTGTGACTCAAACATGGA+AGG - chr1_3:52180661-52180680 None:intergenic 35.0%
! TGTTCTTAGTTTGAGTGAGA+TGG + chr1_3:52180684-52180703 Msa0124400:CDS 35.0%
TACACATGTGTGATATCTCA+TGG + chr1_3:52180721-52180740 Msa0124400:CDS 35.0%
GAAAAAGAGTGAGGACTATT+AGG - chr1_3:52180811-52180830 None:intergenic 35.0%
AGAATGTAGACACAAAGAGA+TGG + chr1_3:52181031-52181050 Msa0124400:CDS 35.0%
ATCTCTATTTGCAAGGTTGA+AGG - chr1_3:52181149-52181168 None:intergenic 35.0%
TGTGCATGAAAATCAAACGT+TGG - chr1_3:52179899-52179918 None:intergenic 35.0%
! GAAAAAGCATGATTTAGCAG+TGG - chr1_3:52180092-52180111 None:intergenic 35.0%
TGTTTCACTTGAGGAGTTTA+AGG - chr1_3:52180177-52180196 None:intergenic 35.0%
AGTCACCAAACAAAGTTTGA+TGG + chr1_3:52180279-52180298 Msa0124400:CDS 35.0%
GTCACCAAACAAAGTTTGAT+GGG + chr1_3:52180280-52180299 Msa0124400:CDS 35.0%
TTCACCCATCAAACTTTGTT+TGG - chr1_3:52180287-52180306 None:intergenic 35.0%
TTTGGTGAACCAAATAGAGA+TGG - chr1_3:52180340-52180359 None:intergenic 35.0%
ACTTCCAAAAAATCTCTCAC+TGG + chr1_3:52180379-52180398 Msa0124400:CDS 35.0%
!!! AGTTCCAGTGAGAGATTTTT+TGG - chr1_3:52180386-52180405 None:intergenic 35.0%
! TGAGAAAGGGTTTTCAAATG+AGG - chr1_3:52180455-52180474 None:intergenic 35.0%
ACCCTTTCTCAATTACCAAT+AGG + chr1_3:52180464-52180483 Msa0124400:CDS 35.0%
GCTACAAACTTTGTTCCTAT+TGG - chr1_3:52180482-52180501 None:intergenic 35.0%
GAACAAAGTTTGTAGCTCAA+GGG + chr1_3:52180486-52180505 Msa0124400:CDS 35.0%
! GATTCAAAAACCATTGGTCT+TGG + chr1_3:52180517-52180536 Msa0124400:CDS 35.0%
! TTTTGTGACTCAAACATGGA+AGG - chr1_3:52180661-52180680 None:intergenic 35.0%
! TGTTCTTAGTTTGAGTGAGA+TGG + chr1_3:52180684-52180703 Msa0124400:CDS 35.0%
TACACATGTGTGATATCTCA+TGG + chr1_3:52180721-52180740 Msa0124400:CDS 35.0%
GAAAAAGAGTGAGGACTATT+AGG - chr1_3:52180811-52180830 None:intergenic 35.0%
AGAATGTAGACACAAAGAGA+TGG + chr1_3:52181031-52181050 Msa0124400:CDS 35.0%
ATCTCTATTTGCAAGGTTGA+AGG - chr1_3:52181149-52181168 None:intergenic 35.0%
GGAACAAAGTTTGTAGCTCA+AGG + chr1_3:52180485-52180504 Msa0124400:CDS 40.0%
!! AAAACCATTGGTCTTGGTCT+TGG + chr1_3:52180523-52180542 Msa0124400:CDS 40.0%
TCTATTTGCAAGGTTGAAGG+AGG - chr1_3:52181146-52181165 None:intergenic 40.0%
GGAACAAAGTTTGTAGCTCA+AGG + chr1_3:52180485-52180504 Msa0124400:CDS 40.0%
!! AAAACCATTGGTCTTGGTCT+TGG + chr1_3:52180523-52180542 Msa0124400:CDS 40.0%
TCTATTTGCAAGGTTGAAGG+AGG - chr1_3:52181146-52181165 None:intergenic 40.0%
AAGACCAAGACCAAGACCAA+TGG - chr1_3:52180530-52180549 None:intergenic 45.0%
TCAAACATGGAAGGTGGTAG+AGG - chr1_3:52180652-52180671 None:intergenic 45.0%
TGTGACTCAAACATGGAAGG+TGG - chr1_3:52180658-52180677 None:intergenic 45.0%
AAGACCAAGACCAAGACCAA+TGG - chr1_3:52180530-52180549 None:intergenic 45.0%
TCAAACATGGAAGGTGGTAG+AGG - chr1_3:52180652-52180671 None:intergenic 45.0%
TGTGACTCAAACATGGAAGG+TGG - chr1_3:52180658-52180677 None:intergenic 45.0%
!! GGTCTTGGTCTTGGTCTTGT+TGG + chr1_3:52180532-52180551 Msa0124400:CDS 50.0%
ACATGGAAGGTGGTAGAGGA+GGG - chr1_3:52180648-52180667 None:intergenic 50.0%
AACATGGAAGGTGGTAGAGG+AGG - chr1_3:52180649-52180668 None:intergenic 50.0%
!! GGTCTTGGTCTTGGTCTTGT+TGG + chr1_3:52180532-52180551 Msa0124400:CDS 50.0%
ACATGGAAGGTGGTAGAGGA+GGG - chr1_3:52180648-52180667 None:intergenic 50.0%
AACATGGAAGGTGGTAGAGG+AGG - chr1_3:52180649-52180668 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 52179898 52181264 52179898 ID=Msa0124400;Name=Msa0124400
chr1_3 mRNA 52179898 52181264 52179898 ID=Msa0124400-mRNA-1;Parent=Msa0124400;Name=Msa0124400-mRNA-1;_AED=0.01;_eAED=0.01;_QI=355|1|1|1|1|1|2|188|239
chr1_3 exon 52179898 52180902 52179898 ID=Msa0124400-mRNA-1:exon:15964;Parent=Msa0124400-mRNA-1
chr1_3 exon 52181007 52181264 52181007 ID=Msa0124400-mRNA-1:exon:15965;Parent=Msa0124400-mRNA-1
chr1_3 five_prime_UTR 52179898 52180252 52179898 ID=Msa0124400-mRNA-1:five_prime_utr;Parent=Msa0124400-mRNA-1
chr1_3 CDS 52180253 52180902 52180253 ID=Msa0124400-mRNA-1:cds;Parent=Msa0124400-mRNA-1
chr1_3 CDS 52181007 52181076 52181007 ID=Msa0124400-mRNA-1:cds;Parent=Msa0124400-mRNA-1
chr1_3 three_prime_UTR 52181077 52181264 52181077 ID=Msa0124400-mRNA-1:three_prime_utr;Parent=Msa0124400-mRNA-1
Gene Sequence

>Msa0124400

ATGCTACTCAGAAACAGATCAAGAGCAGTCACCAAACAAAGTTTGATGGGTGAACAATCACCAAATCAAAATTTCACTACAATACCATCTCTATTTGGTTCACCAAACTATAGAGATTTTCATTTCACTTCCAAAAAATCTCTCACTGGAACTGAAGCTTTGATAAGTCCAACATCAATTCTTGACACAAAAGCGTTAGCCTCATTTGAAAACCCTTTCTCAATTACCAATAGGAACAAAGTTTGTAGCTCAAGGGACAAAGTAGATTCAAAAACCATTGGTCTTGGTCTTGGTCTTGTTGGTGTTCTGAAAGATGAACCTTTTCATCAAAATTCTGATCATAAACTAAGCAATAGAAAAGTTCTGTTTGGAACTGAGCTTAGAGTGAAAATCCCTCCTCTACCACCTTCCATGTTTGAGTCACAAAAAAGTGTTCTTAGTTTGAGTGAGATGGAACTTTCTGAAGAATACACATGTGTGATATCTCATGGACCAAACCCAAAAACAACTCATATATTTGATAATTGTGTTGTGGAAGAAAGTTACTGTTCTGTCCCTAATAGTCCTCACTCTTTTTCACTGAATTTTCTTAGCTTTTGTTACACTTGCAAAAATCATCTTGAGCATACAAAAGACATCTTCATTTACAGAGGAGAAAAAGCTTTTTGCAGTCAAGAATGTAGACACAAAGAGATGGTGTTAGATGAAGCAGAAAATTAA

Protein sequence

>Msa0124400

MLLRNRSRAVTKQSLMGEQSPNQNFTTIPSLFGSPNYRDFHFTSKKSLTGTEALISPTSILDTKALASFENPFSITNRNKVCSSRDKVDSKTIGLGLGLVGVLKDEPFHQNSDHKLSNRKVLFGTELRVKIPPLPPSMFESQKSVLSLSEMELSEEYTCVISHGPNPKTTHIFDNCVVEESYCSVPNSPHSFSLNFLSFCYTCKNHLEHTKDIFIYRGEKAFCSQECRHKEMVLDEAEN*