AlfalfaGEDB | Msa0124530

M. sativa cultivar zhongmu-4 / Msa0124530

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0124530	sp\|Q8S3D5\|LRL2_ARATH	36.923	130	80	1	190	317	136	265	1.62e-17	84.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0124530	G7IEF8	92.615	325	24	0	1	325	1	325	0.0	628

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TFs/TRs:

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Gene ID	Type	Classification
Msa0124530	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0029060	Msa0124530	0.987602	7.444107e-171	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0124530	MtrunA17_Chr1g0185741	91.749	303	25	0	1	303	1	303	0.0	583
Msa0124530	MtrunA17_Chr4g0074161	36.486	148	74	3	190	317	212	359	4.14e-18	84.3
Msa0124530	MtrunA17_Chr1g0190731	34.307	137	78	1	190	314	199	335	4.73e-16	77.8
Msa0124530	MtrunA17_Chr1g0184841	34.286	140	80	1	190	317	126	265	1.02e-11	64.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0124530	AT5G64980.1	43.089	123	68	2	191	311	218	340	7.94e-22	94.7
Msa0124530	AT5G64980.2	43.089	123	68	2	191	311	218	340	7.94e-22	94.7
Msa0124530	AT4G30980.1	36.923	130	80	1	190	317	136	265	1.65e-18	84.7
Msa0124530	AT4G30980.2	36.923	130	80	1	190	317	184	313	2.58e-18	85.1
Msa0124530	AT2G24260.2	34.132	167	83	5	170	317	23	181	4.20e-18	82.8
Msa0124530	AT2G24260.1	34.731	167	82	5	170	317	126	284	1.85e-17	82.4
Msa0124530	AT5G58010.1	33.784	148	74	4	190	315	105	250	7.27e-15	74.3
Msa0124530	AT1G03040.2	30.457	197	117	4	133	311	79	273	4.70e-13	68.9
Msa0124530	AT1G03040.1	36.434	129	75	1	190	311	150	278	1.87e-12	67.4
Msa0124530	AT4G02590.3	34.559	136	75	1	190	311	89	224	1.71e-11	63.9

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Find 80 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAAGGGTTAAAACTTAA+AGG	0.276505	1_3:-52351176	None:intergenic
AAACGACAGATTACCACATC+GGG	0.526123	1_3:-52352755	None:intergenic
AAAGGCTTAAAAGGGTATCA+TGG	0.502625	1_3:-52351085	None:intergenic
AAATAGTTAGCTAGCTAGCT+TGG	0.466352	1_3:-52353736	None:intergenic
AACCAACACTTTGGAAGAAG+TGG	0.489754	1_3:+52351322	Msa0124530:CDS
AACTGAACCAAGCTATGCAA+TGG	0.660513	1_3:+52353708	Msa0124530:CDS
AAGTAGCCATGATGTTTCTG+AGG	0.620893	1_3:+52351285	Msa0124530:CDS
AAGTTAGAGAAAGAAAGGTA+TGG	0.549135	1_3:-52351046	None:intergenic
AATAGCCATTATCACACTCT+TGG	0.527667	1_3:+52351460	Msa0124530:CDS
ACAACTGAGTATAATATGAT+TGG	0.400574	1_3:+52351436	Msa0124530:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGTTAGAGAAAGAAAGGTA+TGG	-	chr1_3:52351049-52351068	None:intergenic	30.0%
GAGTAATGAAAAAGTGGTAA+AGG	-	chr1_3:52351147-52351166	None:intergenic	30.0%
GAAGTTGAGTAATGAAAAAG+TGG	-	chr1_3:52351153-52351172	None:intergenic	30.0%
GTAGTGAAAACAAAAATGGA+AGG	+	chr1_3:52351205-52351224	Msa0124530:exon	30.0%
CAAATTCAAAACCAACACTT+TGG	+	chr1_3:52351313-52351332	Msa0124530:CDS	30.0%
AGGAAGGTAAAGTTAATGAA+TGG	+	chr1_3:52351568-52351587	Msa0124530:intron	30.0%
ATTAACTAACCTCAGTATAG+TGG	-	chr1_3:52351764-52351783	None:intergenic	30.0%
AATCTATCTTGAAGAGCTTA+TGG	+	chr1_3:52352049-52352068	Msa0124530:intron	30.0%
TATGGAAACGTGAAACTATT+TGG	-	chr1_3:52352264-52352283	None:intergenic	30.0%
TGCTTCACAATTTGATCATA+TGG	+	chr1_3:52352408-52352427	Msa0124530:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	52350977	52353991	52350977	ID=Msa0124530;Name=Msa0124530
chr1_3	mRNA	52350977	52353991	52350977	ID=Msa0124530-mRNA-1;Parent=Msa0124530;Name=Msa0124530-mRNA-1;_AED=0.01;_eAED=0.01;_QI=243\|1\|1\|1\|1\|1\|7\|243\|325
chr1_3	exon	52350977	52351573	52350977	ID=Msa0124530-mRNA-1:exon:16022;Parent=Msa0124530-mRNA-1
chr1_3	exon	52351687	52351773	52351687	ID=Msa0124530-mRNA-1:exon:16023;Parent=Msa0124530-mRNA-1
chr1_3	exon	52352364	52352522	52352364	ID=Msa0124530-mRNA-1:exon:16024;Parent=Msa0124530-mRNA-1
chr1_3	exon	52352698	52352763	52352698	ID=Msa0124530-mRNA-1:exon:16025;Parent=Msa0124530-mRNA-1
chr1_3	exon	52352915	52352980	52352915	ID=Msa0124530-mRNA-1:exon:16026;Parent=Msa0124530-mRNA-1
chr1_3	exon	52353131	52353190	52353131	ID=Msa0124530-mRNA-1:exon:16027;Parent=Msa0124530-mRNA-1
chr1_3	exon	52353563	52353991	52353563	ID=Msa0124530-mRNA-1:exon:16028;Parent=Msa0124530-mRNA-1
chr1_3	five_prime_UTR	52350977	52351219	52350977	ID=Msa0124530-mRNA-1:five_prime_utr;Parent=Msa0124530-mRNA-1
chr1_3	CDS	52351220	52351573	52351220	ID=Msa0124530-mRNA-1:cds;Parent=Msa0124530-mRNA-1
chr1_3	CDS	52351687	52351773	52351687	ID=Msa0124530-mRNA-1:cds;Parent=Msa0124530-mRNA-1
chr1_3	CDS	52352364	52352522	52352364	ID=Msa0124530-mRNA-1:cds;Parent=Msa0124530-mRNA-1
chr1_3	CDS	52352698	52352763	52352698	ID=Msa0124530-mRNA-1:cds;Parent=Msa0124530-mRNA-1
chr1_3	CDS	52352915	52352980	52352915	ID=Msa0124530-mRNA-1:cds;Parent=Msa0124530-mRNA-1
chr1_3	CDS	52353131	52353190	52353131	ID=Msa0124530-mRNA-1:cds;Parent=Msa0124530-mRNA-1
chr1_3	CDS	52353563	52353748	52353563	ID=Msa0124530-mRNA-1:cds;Parent=Msa0124530-mRNA-1
chr1_3	three_prime_UTR	52353749	52353991	52353749	ID=Msa0124530-mRNA-1:three_prime_utr;Parent=Msa0124530-mRNA-1

Gene Sequence

>Msa0124530

ATGGAAGGAGAAAGTAACAACAAAGAGAACAGTTCAGAGCAACTTTCATATGAAAATGAAGGAACAAGTAGCCATGATGTTTCTGAGGAACATCAAATTCAAAACCAACACTTTGGAAGAAGTGGGATAGAGTGGAATTTCTTACCAAAACTACCTCAAAATAACTATGTTGAATATCTCTCTGAATCCTCATGCTATCTTCCAAACACTACTACAACAACTGAGTATAATATGATTGGGAATAGCCATTATCACACTCTTGGTAGTAGCAGCAGCAGTTTCAATGATCCTGGAAAAGCTGATTTTGGTTTTAAGCTGATTGATTCTTCAAGTCATGATTTTGGTGTCAGGAAGCGTGTAGGGTTTTGGAGAAATGATGAAGGAGAGGAAGAAGCTCATATATTTGAAACTGGAAGTAGTCAAAATCTCCACTATACTGAGGGTCATGCAACATGGACACCCGATTCAGTTGGAGATAAGCAATATGCTTCACAATTTGATCATATGGGAGTAGTTGCTGCACCTTCTTTACTTCCAATTCCAAAATCAGGAGGCAGCTACACAAAACAGAAAACTGAGAAAACACGTTACAGTGACCGTCAACGCAGGCAACGTATCGCTGATAACCTCAAAGCTTTACATGAATTGCTGCCGACTCCCGATGTGGGTAGTCAAGCTTACATATTGGATGATATCATTGACTATGTTAAATACTTGCAGATTCAAGTGAAGGAACTAAGTGGAAGTAAATTGCAAGCTGATTCAAATGCTATACCACTAGTTTTCCATGAGGGTTATGGGCATTATATAAAGGAACAGATTTTGAATGAGCCTCTTGAAGAGATAATGGGGAAACTAATAGAAGTGAATTCAGCAGAAACATGTCAGCTACTTGAAAACAAAGGTCTTGTTCTGCTGCCTATTGCTTTGGTTGATGAACTGAACCAAGCTATGCAATGGTTGAACCAAGCTAGCTAG

Protein sequence

>Msa0124530

MEGESNNKENSSEQLSYENEGTSSHDVSEEHQIQNQHFGRSGIEWNFLPKLPQNNYVEYLSESSCYLPNTTTTTEYNMIGNSHYHTLGSSSSSFNDPGKADFGFKLIDSSSHDFGVRKRVGFWRNDEGEEEAHIFETGSSQNLHYTEGHATWTPDSVGDKQYASQFDHMGVVAAPSLLPIPKSGGSYTKQKTEKTRYSDRQRRQRIADNLKALHELLPTPDVGSQAYILDDIIDYVKYLQIQVKELSGSKLQADSNAIPLVFHEGYGHYIKEQILNEPLEEIMGKLIEVNSAETCQLLENKGLVLLPIALVDELNQAMQWLNQAS*