AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0126950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0126950 MtrunA17_Chr1g0189851 94.828 638 3 1 1 638 137 744 0.0 1257
Msa0126950 MtrunA17_Chr1g0189851 34.848 132 86 0 1 132 1 132 3.74e-14 75.9
Msa0126950 MtrunA17_Chr1g0189851 31.897 116 79 0 36 151 2 117 1.79e-11 67.4
Msa0126950 MtrunA17_Chr1g0178301 25.801 624 366 13 9 622 317 853 4.37e-50 187
Msa0126950 MtrunA17_Chr1g0189861 37.864 103 64 0 30 132 102 204 3.91e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0126950 AT3G04240.1 81.348 638 89 1 1 638 370 977 0.0 1115
Msa0126950 AT3G04240.1 35.606 132 85 0 1 132 234 365 8.58e-16 82.0
Msa0126950 AT3G04240.1 32.331 133 90 0 5 137 204 336 2.68e-15 80.5
Msa0126950 AT3G04240.1 32.258 124 84 0 9 132 140 263 1.11e-14 78.2
Msa0126950 AT3G04240.2 81.348 638 89 1 1 638 137 744 0.0 1114
Msa0126950 AT3G04240.2 35.606 132 85 0 1 132 1 132 5.82e-16 82.4
Msa0126950 AT3G04240.2 36.275 102 65 0 36 137 2 103 1.28e-12 71.6
Msa0126950 AT3G11540.1 25.392 638 381 14 9 637 314 865 1.51e-43 168
Msa0126950 AT3G11540.1 32.117 137 87 1 2 132 124 260 9.41e-11 65.5
Msa0126950 AT3G11540.2 26.887 424 260 9 223 637 301 683 3.69e-35 142

Find 229 sgRNAs with CRISPR-Local

Find 2 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAATCCTTATCAACCTTAA+TGG 0.143162 1_3:+56095307 Msa0126950:CDS
TCAAAATTGGGTCGCGTATT+TGG 0.156837 1_3:+56098392 Msa0126950:three_prime_UTR
GTAATTTAGTTACTCAAAAT+TGG 0.160570 1_3:+56098379 Msa0126950:three_prime_UTR
GGCTGAAGCTCATGCTAATT+TGG 0.170309 1_3:+56092479 Msa0126950:CDS
TACCATGTATTAAATATTTC+AGG 0.201953 1_3:-56097008 None:intergenic
GGGCGGGTTGTGATGGGTTT+CGG 0.205890 1_3:+56098617 Msa0126950:three_prime_UTR
GTCTCGGATTTAGCGGGTTT+CGG 0.216426 1_3:-56098579 None:intergenic
GGCGGGTTGTGATGGGTTTC+GGG 0.225983 1_3:+56098618 Msa0126950:three_prime_UTR
CCACAGGAACAGATATATTA+TGG 0.228996 1_3:+56097665 Msa0126950:CDS
CAATGCTTGCTCTGGAAATT+AGG 0.230192 1_3:+56094668 Msa0126950:CDS
ACCAGTTCAAGTTTCATATA+TGG 0.238851 1_3:+56096537 Msa0126950:CDS
TCTCGGATTTAGCGGGTTTC+GGG 0.240794 1_3:-56098578 None:intergenic
TTACAACAATGTATATTCTT+TGG 0.248808 1_3:-56098360 None:intergenic
TACTCTGCCTCTTGAGAAAA+TGG 0.256247 1_3:+56097705 Msa0126950:CDS
GTAGGCCTGTCTAGATTAAT+AGG 0.262820 1_3:-56098822 None:intergenic
GCAACGGTCGGGTCCGGTTT+TGG 0.270470 1_3:+56098675 Msa0126950:three_prime_UTR
ATAGTATATTTGTACCTATT+CGG 0.271503 1_3:-56098728 None:intergenic
CTACCTTTGTATAACCATTA+AGG 0.273432 1_3:-56095321 None:intergenic
TAATTTGGCTTCTGCCTATA+AGG 0.274347 1_3:+56092494 Msa0126950:CDS
TCATTCCTGTCGGTAATATT+AGG 0.282505 1_3:+56098532 Msa0126950:three_prime_UTR
ATTCGGGCGGGTGTGGTTAC+CGG 0.289749 1_3:-56098711 None:intergenic
ATTAGCATACCTATCCTTAT+AGG 0.292089 1_3:-56092508 None:intergenic
CATGTATATATTTCCAAGAT+TGG 0.315239 1_3:-56091927 None:intergenic
TAACCTCCCTCCTGCTTGAT+TGG 0.315790 1_3:-56094865 None:intergenic
GCGTATTTGGTTACAGTTGT+TGG 0.317267 1_3:+56098405 Msa0126950:three_prime_UTR
TCAGTTAGAGCTTGAAGCTT+TGG 0.317430 1_3:-56097990 None:intergenic
GTTTCATATATGGGATTTCC+TGG 0.320555 1_3:+56096547 Msa0126950:CDS
CCAGTTCAAGTTTCATATAT+GGG 0.320872 1_3:+56096538 Msa0126950:CDS
CTCTGTATTTCCACTGGACT+GGG 0.331322 1_3:+56097748 Msa0126950:CDS
AGAAGATTGCAAGTTGCTTC+AGG 0.349834 1_3:-56092905 None:intergenic
TAGTATATTTGTACCTATTC+GGG 0.351596 1_3:-56098727 None:intergenic
TCCTATGTCTATTTATCATC+TGG 0.352528 1_3:-56098252 None:intergenic
CTCCATTCAGTACCATCATT+TGG 0.353434 1_3:-56095193 None:intergenic
CAGTTAGAGCTTGAAGCTTT+GGG 0.354805 1_3:-56097989 None:intergenic
GATCGTTTGGGCTGACAGTT+TGG 0.362406 1_3:-56096921 None:intergenic
GTGATGCGGGCGGGTTGTGA+TGG 0.362774 1_3:+56098610 Msa0126950:three_prime_UTR
AAACAGAGGCAATACTTATA+AGG 0.364549 1_3:+56092392 Msa0126950:CDS
CTCTTGAAATTTCCTGCAGC+AGG 0.374684 1_3:+56097179 Msa0126950:CDS
CAATCTTCTACATACACTTC+AGG 0.378736 1_3:+56092919 Msa0126950:CDS
CCACAAGCGTTGACCAATCT+TGG 0.379245 1_3:+56091914 Msa0126950:five_prime_UTR
CTTTGTAAACGATTATAAAC+AGG 0.383696 1_3:+56096773 Msa0126950:CDS
ATTATAGCAAGGTTGTTATA+CGG 0.385320 1_3:-56092107 None:intergenic
TATAACCATTAAGGTTGATA+AGG 0.388324 1_3:-56095312 None:intergenic
ATAGGAGCAGGATGGCTAAA+TGG 0.389099 1_3:-56094841 None:intergenic
TATTAGAAGATGTTTGCTTT+TGG 0.391927 1_3:+56098480 Msa0126950:three_prime_UTR
CAGCCCAAACGATCAGATTA+TGG 0.392917 1_3:+56096930 Msa0126950:CDS
ACTCTGTATTTCCACTGGAC+TGG 0.395288 1_3:+56097747 Msa0126950:CDS
CGTATTTGGTTACAGTTGTT+GGG 0.396559 1_3:+56098406 Msa0126950:three_prime_UTR
TCCATTTCACATACCCTTCA+CGG 0.400822 1_3:-56095508 None:intergenic
CAAATGTTCAAGGAAGTAGA+AGG 0.411950 1_3:+56094456 Msa0126950:CDS
CTTCACAGATGTTGCAATGA+AGG 0.419095 1_3:+56097470 Msa0126950:CDS
TGGATTTCAATTTCCAAAAC+CGG 0.423910 1_3:-56098688 None:intergenic
TAATTTAGTTACTCAAAATT+GGG 0.424955 1_3:+56098380 Msa0126950:three_prime_UTR
GAGACCATAATCTGATCGTT+TGG 0.428281 1_3:-56096934 None:intergenic
CTCTTGAGAAAATGGCTACT+AGG 0.429756 1_3:+56097713 Msa0126950:CDS
CAAGATTGGTCAACGCTTGT+GGG 0.432953 1_3:-56091913 None:intergenic
ATGCTATGGCATGGAAAGGC+TGG 0.434506 1_3:-56094633 None:intergenic
ATCTGAAACAGAGCACTTTG+TGG 0.435863 1_3:+56095228 Msa0126950:CDS
GTAACCACACCCGCCCGAAT+AGG 0.440304 1_3:+56098714 Msa0126950:three_prime_UTR
CCAAGATTGGTCAACGCTTG+TGG 0.445603 1_3:-56091914 None:intergenic
AAAATGGCTACTAGGGTTGC+TGG 0.449044 1_3:+56097721 Msa0126950:CDS
TGATGCGGGCGGGTTGTGAT+GGG 0.452474 1_3:+56098611 Msa0126950:three_prime_UTR
TGTACCTATTCGGGCGGGTG+TGG 0.455240 1_3:-56098718 None:intergenic
CACAGGAACAGATATATTAT+GGG 0.455571 1_3:+56097666 Msa0126950:CDS
CCCATATATGAAACTTGAAC+TGG 0.456705 1_3:-56096538 None:intergenic
CTTTCAGATGCTGCTGCACA+AGG 0.457171 1_3:+56097426 Msa0126950:intron
GACCTCATTGTAGCAAGATA+TGG 0.458721 1_3:-56092334 None:intergenic
AGCAGTGTGCAGCATATTTG+CGG 0.459706 1_3:-56094789 None:intergenic
GGCAATACTTATAAGGAGAT+TGG 0.459746 1_3:+56092399 Msa0126950:CDS
ATGGGATTTCCTGGCACAAC+CGG 0.462984 1_3:+56096556 Msa0126950:CDS
CCCTCCTGCTTGATTGGAAT+AGG 0.463550 1_3:-56094859 None:intergenic
CGGATTTAGCGGGTTTCGGG+CGG 0.463780 1_3:-56098575 None:intergenic
AGACCATAATCTGATCGTTT+GGG 0.464870 1_3:-56096933 None:intergenic
TAATGCAGTGTGTCTGTTGT+TGG 0.467039 1_3:+56094421 Msa0126950:intron
GGGTTTCGGGTCTGCAAATG+AGG 0.468893 1_3:+56098631 Msa0126950:three_prime_UTR
TGGATAGCAACGGTCGGGTC+CGG 0.470263 1_3:+56098669 Msa0126950:three_prime_UTR
GGCTCACTCTGTATTTCCAC+TGG 0.472212 1_3:+56097742 Msa0126950:CDS
CAAGAGCCAAAGCGCGCTGT+TGG 0.474016 1_3:-56097162 None:intergenic
ACTTGACCCAATGCTTGCTC+TGG 0.474813 1_3:+56094660 Msa0126950:CDS
TAGAGTCAGTGATGCTATCC+AGG 0.479729 1_3:+56092422 Msa0126950:CDS
CTACATTCCCCTCTCTAATA+CGG 0.479761 1_3:-56098278 None:intergenic
CCACCCATTGTCACATCTCA+TGG 0.482380 1_3:+56095026 Msa0126950:CDS
AAGAGCCAAAGCGCGCTGTT+GGG 0.483592 1_3:-56097161 None:intergenic
TATGAAACTTGAACTGGTGC+AGG 0.484055 1_3:-56096532 None:intergenic
GCAAACCTATTAATCTAGAC+AGG 0.484652 1_3:+56098817 Msa0126950:three_prime_UTR
TTGGCAACCCTCAGACATGC+AGG 0.486108 1_3:-56098332 None:intergenic
GTAATCTATGTATGTAGCTC+CGG 0.486466 1_3:-56096575 None:intergenic
GGTTATGAAAGACTAAGGAT+TGG 0.487687 1_3:+56094883 Msa0126950:CDS
CTGTGATGGGTGATGCGGGC+GGG 0.489583 1_3:+56098601 Msa0126950:three_prime_UTR
GGATTTAGCGGGTTTCGGGC+GGG 0.489821 1_3:-56098574 None:intergenic
AGCTACATACATAGATTACT+TGG 0.496829 1_3:+56096579 Msa0126950:CDS
CTTCAATCAACTTTACAAGA+TGG 0.496856 1_3:+56096983 Msa0126950:CDS
CAAAGTAGCAATGCGGGAGA+TGG 0.497765 1_3:-56096755 None:intergenic
TTCAGATGCTGCTGCACAAG+GGG 0.497876 1_3:+56097428 Msa0126950:intron
TAATTTCCAGAGCAAGCATT+GGG 0.501643 1_3:-56094666 None:intergenic
TATGTAGCTCCGGTTGTGCC+AGG 0.502858 1_3:-56096565 None:intergenic
GTTATGAAAGACTAAGGATT+GGG 0.503729 1_3:+56094884 Msa0126950:CDS
TCTGTGAAGATAATTTGATC+AGG 0.505192 1_3:-56097456 None:intergenic
AGTCTCATTTCTCCTGCTGC+AGG 0.505262 1_3:-56097191 None:intergenic
TGCTTGATTGGAATAGGAGC+AGG 0.506321 1_3:-56094853 None:intergenic
GGTCCTCCGCATTGATCCCT+TGG 0.509269 1_3:+56092353 Msa0126950:CDS
ATTGATCCCTTGGCAGCTGA+TGG 0.509463 1_3:+56092363 Msa0126950:CDS
AATGCAGTGTGTCTGTTGTT+GGG 0.510745 1_3:+56094422 Msa0126950:intron
TGCTATAATATATAAGCAGC+AGG 0.515077 1_3:+56092121 Msa0126950:CDS
GCATGGAAAGGCTGGACACT+AGG 0.517313 1_3:-56094625 None:intergenic
ATCACAGTCTCGGATTTAGC+GGG 0.517323 1_3:-56098585 None:intergenic
CGTTTACAAAGTAGCAATGC+GGG 0.517564 1_3:-56096761 None:intergenic
TAATCGCACGTATGTAATCC+TGG 0.519833 1_3:-56092440 None:intergenic
CAATCTTGGAAATATATACA+TGG 0.519958 1_3:+56091928 Msa0126950:exon
TAAATAAAAGTACTAATACT+TGG 0.520112 1_3:-56098875 None:intergenic
TGCTATCGTATCTGATGTCA+TGG 0.520898 1_3:-56095260 None:intergenic
ACATGCAGCTGATGTCTAGT+AGG 0.521344 1_3:-56098840 None:intergenic
TACAAGACCATCAGCTGCCA+AGG 0.521518 1_3:-56092370 None:intergenic
ACACATTAGACGGAGTTCCT+TGG 0.522719 1_3:+56097497 Msa0126950:CDS
GTGTACCCAACAGCGCGCTT+TGG 0.522743 1_3:+56097156 Msa0126950:CDS
TCCGTGAAGGGTATGTGAAA+TGG 0.525124 1_3:+56095507 Msa0126950:CDS
CGCTAAATCCGAGACTGTGA+TGG 0.526789 1_3:+56098587 Msa0126950:three_prime_UTR
CTAATTTCCAGAGCAAGCAT+TGG 0.530198 1_3:-56094667 None:intergenic
CGCATCACCCATCACAGTCT+CGG 0.531563 1_3:-56098595 None:intergenic
TTCACAGATGTTGCAATGAA+GGG 0.531625 1_3:+56097471 Msa0126950:CDS
CTGTATTTCCACTGGACTGG+GGG 0.534881 1_3:+56097750 Msa0126950:CDS
AGTACCATCATTTGGACTCA+AGG 0.534981 1_3:-56095185 None:intergenic
CTCTCTTTGACACAAACCGA+TGG 0.535023 1_3:+56098042 Msa0126950:CDS
TGGCTTCTGCCTATAAGGAT+AGG 0.535459 1_3:+56092499 Msa0126950:CDS
TTTAGTACTTTGATCCGTGA+AGG 0.536373 1_3:+56095494 Msa0126950:CDS
CATCACAGTCTCGGATTTAG+CGG 0.536405 1_3:-56098586 None:intergenic
TTGGAAATATATACATGGAA+TGG 0.538270 1_3:+56091933 Msa0126950:exon
AGGGAGGTTATGAAAGACTA+AGG 0.540074 1_3:+56094878 Msa0126950:CDS
ATGAGTGGATAGCAACGGTC+GGG 0.540112 1_3:+56098664 Msa0126950:three_prime_UTR
AAGAACATACATTATAGCAC+TGG 0.540515 1_3:-56091776 None:intergenic
TCTTGAGAAAATGGCTACTA+GGG 0.544333 1_3:+56097714 Msa0126950:CDS
GTGGTATGTGCATTGCATAA+AGG 0.548073 1_3:-56097646 None:intergenic
TAGTTAACCCTGCATGTCTG+AGG 0.548874 1_3:+56098324 Msa0126950:three_prime_UTR
TGCTCCTATTCCAATCAAGC+AGG 0.555496 1_3:+56094855 Msa0126950:CDS
ATCTGCATTGCTGTGGTCAA+CGG 0.555622 1_3:+56098190 Msa0126950:CDS
TTTCAGATGCTGCTGCACAA+GGG 0.555667 1_3:+56097427 Msa0126950:intron
GGTAATGCTCTGAAAGATGT+TGG 0.562829 1_3:+56091735 Msa0126950:five_prime_UTR
ATTATGGGCAGGTCTGCCTA+TGG 0.564345 1_3:+56097681 Msa0126950:CDS
GGAACAGATATATTATGGGC+AGG 0.566521 1_3:+56097670 Msa0126950:CDS
TGGGTCAAGTGGATATGCTA+TGG 0.567625 1_3:-56094647 None:intergenic
GAGACTGTGATGGGTGATGC+GGG 0.570873 1_3:+56098597 Msa0126950:three_prime_UTR
TTCAGCCATGGTAGGCCTGA+CGG 0.571461 1_3:-56092463 None:intergenic
AGAGCAAGCATTGGGTCAAG+TGG 0.574030 1_3:-56094658 None:intergenic
GGCTGTAAAGAGCTACAGAC+AGG 0.576286 1_3:+56092859 Msa0126950:CDS
GCTACACTAAATGTAACCAC+TGG 0.577805 1_3:+56092074 Msa0126950:CDS
TTATTTCAGTGGACATGTAG+AGG 0.579165 1_3:+56092835 Msa0126950:intron
ATCCTGAAATATTTAATACA+TGG 0.581813 1_3:+56097006 Msa0126950:CDS
GGGAGGAGATGATTGTCAGC+AGG 0.582027 1_3:+56097770 Msa0126950:CDS
GGATCGTGATCAAATGTTCA+AGG 0.582624 1_3:+56094446 Msa0126950:CDS
ATCCCATGAGATGTGACAAT+GGG 0.592439 1_3:-56095029 None:intergenic
AGATTACTTGGTCACTGATG+AGG 0.592637 1_3:+56096591 Msa0126950:CDS
TAGATATGTGCATACTGAAG+AGG 0.593648 1_3:-56096717 None:intergenic
TCAAGAGGCAGAGTAACCAT+AGG 0.593677 1_3:-56097697 None:intergenic
GTCAACGCTTGTGGGTGGTT+AGG 0.593743 1_3:-56091905 None:intergenic
TCAGTGACCTTAAAATGCTG+TGG 0.594451 1_3:-56098213 None:intergenic
CAAGTGGATATGCTATGGCA+TGG 0.594989 1_3:-56094642 None:intergenic
TATATTTGTACCTATTCGGG+CGG 0.595659 1_3:-56098724 None:intergenic
CAACCTTAATGGTTATACAA+AGG 0.596920 1_3:+56095318 Msa0126950:CDS
AGCAGGAGAAATGAGACTGA+GGG 0.597842 1_3:+56097196 Msa0126950:CDS
GGTGGTTAGGCTGTAACGAA+AGG 0.598171 1_3:-56091892 None:intergenic
TCAGTCCTAATATTACCGAC+AGG 0.598237 1_3:-56098537 None:intergenic
GTTTGTGTCAAAGAGAGGAC+AGG 0.598476 1_3:-56098036 None:intergenic
TAGGAATACCGTATTAGAGA+GGG 0.598539 1_3:+56098270 Msa0126950:three_prime_UTR
TTATTTAACACAGGCTCCGA+AGG 0.598976 1_3:-56098509 None:intergenic
TGAAGGGTGAACACATTAGA+CGG 0.599057 1_3:+56097487 Msa0126950:CDS
ACATACGTGCGATTACCGTC+AGG 0.600155 1_3:+56092448 Msa0126950:CDS
AGTTAACCCTGCATGTCTGA+GGG 0.603613 1_3:+56098325 Msa0126950:three_prime_UTR
CAGGAATGATTATTTAACAC+AGG 0.603830 1_3:-56098518 None:intergenic
TCCAGATGATAAATAGACAT+AGG 0.604142 1_3:+56098251 Msa0126950:exon
CCATAATATATCTGTTCCTG+TGG 0.606701 1_3:-56097665 None:intergenic
CACCCATTGTCACATCTCAT+GGG 0.607132 1_3:+56095027 Msa0126950:CDS
TCTGTATTTCCACTGGACTG+GGG 0.607244 1_3:+56097749 Msa0126950:CDS
GCTGCTTATATATTATAGCA+AGG 0.607657 1_3:-56092118 None:intergenic
TCCTATTCCAATCAAGCAGG+AGG 0.607994 1_3:+56094858 Msa0126950:CDS
TTCAAAAGTATGATTTGACA+AGG 0.609596 1_3:-56098788 None:intergenic
ATATTTGTACCTATTCGGGC+GGG 0.611260 1_3:-56098723 None:intergenic
GCTAAATCCGAGACTGTGAT+GGG 0.612449 1_3:+56098588 Msa0126950:three_prime_UTR
TGGCAACCCTCAGACATGCA+GGG 0.615615 1_3:-56098331 None:intergenic
CGGGCGGGTGTGGTTACCGG+TGG 0.615623 1_3:-56098708 None:intergenic
GATTGGTCAACGCTTGTGGG+TGG 0.615687 1_3:-56091910 None:intergenic
GTCCAAATGATGGTACTGAA+TGG 0.616666 1_3:+56095191 Msa0126950:CDS
TTAGTACTTTGATCCGTGAA+GGG 0.617988 1_3:+56095495 Msa0126950:CDS
TATGAGTGGATAGCAACGGT+CGG 0.619351 1_3:+56098663 Msa0126950:three_prime_UTR
TTATGCAATGCACATACCAC+AGG 0.622011 1_3:+56097649 Msa0126950:CDS
GTCAAGAAATAAGTCTGCCA+AGG 0.623121 1_3:-56097514 None:intergenic
GCATGAGCTTCAGCCATGGT+AGG 0.623825 1_3:-56092471 None:intergenic
CATCGGTTTGTGTCAAAGAG+AGG 0.626631 1_3:-56098041 None:intergenic
TGAGGGAATTCAGTTATGAG+TGG 0.628533 1_3:+56098649 Msa0126950:three_prime_UTR
AAATGTTCAAGGAAGTAGAA+GGG 0.629997 1_3:+56094457 Msa0126950:CDS
GAAAGATGTTGGCAGAGTGG+AGG 0.630480 1_3:+56091746 Msa0126950:five_prime_UTR
TCTCTTTGACACAAACCGAT+GGG 0.631347 1_3:+56098043 Msa0126950:CDS
CGAGACTGTGATGGGTGATG+CGG 0.632931 1_3:+56098596 Msa0126950:three_prime_UTR
TGCCATATCTTGCTACAATG+AGG 0.633633 1_3:+56092332 Msa0126950:CDS
ATAGGAATACCGTATTAGAG+AGG 0.635817 1_3:+56098269 Msa0126950:three_prime_UTR
TATGCACAACAGAAAGAATG+TGG 0.638579 1_3:+56095062 Msa0126950:CDS
GTGAAGGGTATGTGAAATGG+AGG 0.642881 1_3:+56095510 Msa0126950:CDS
GGTTTCGGGTCTGCAAATGA+GGG 0.644012 1_3:+56098632 Msa0126950:three_prime_UTR
ATTAGCATGAGCTTCAGCCA+TGG 0.646265 1_3:-56092475 None:intergenic
AGGTATGTGAGTAGTGACTT+TGG 0.648824 1_3:+56095000 Msa0126950:intron
CAGCAGGAGAAATGAGACTG+AGG 0.649017 1_3:+56097195 Msa0126950:CDS
CAGTTATGAGTGGATAGCAA+CGG 0.650669 1_3:+56098659 Msa0126950:three_prime_UTR
TGTTAAATAATCATTCCTGT+CGG 0.652848 1_3:+56098522 Msa0126950:three_prime_UTR
GATTACCGTCAGGCCTACCA+TGG 0.652850 1_3:+56092458 Msa0126950:CDS
ACAAGACCATCAGCTGCCAA+GGG 0.656374 1_3:-56092369 None:intergenic
GGATATGCTATGGCATGGAA+AGG 0.659767 1_3:-56094637 None:intergenic
ACTGTGATGGGTGATGCGGG+CGG 0.661702 1_3:+56098600 Msa0126950:three_prime_UTR
CTGCCAAGGGATCAATGCGG+AGG 0.661848 1_3:-56092356 None:intergenic
CCTATTCCAATCAAGCAGGA+GGG 0.662777 1_3:+56094859 Msa0126950:CDS
CCATGAGATGTGACAATGGG+TGG 0.665060 1_3:-56095026 None:intergenic
ATTCCAATCAAGCAGGAGGG+AGG 0.666622 1_3:+56094862 Msa0126950:CDS
TATGCCTTGAGTCCAAATGA+TGG 0.671518 1_3:+56095181 Msa0126950:CDS
ATGTAGATAAGAAAATCAAG+AGG 0.673481 1_3:+56098295 Msa0126950:three_prime_UTR
GCAGTGTGTCTGTTGTTGGG+AGG 0.677864 1_3:+56094425 Msa0126950:intron
AAATTGTTAATGCAGGAACA+TGG 0.679005 1_3:+56092025 Msa0126950:intron
GGAGCAGGATGGCTAAATGG+TGG 0.680480 1_3:-56094838 None:intergenic
TTTCAGTGGACATGTAGAGG+CGG 0.686039 1_3:+56092838 Msa0126950:intron
GATCCCATGAGATGTGACAA+TGG 0.693395 1_3:-56095030 None:intergenic
AGGAATACCGTATTAGAGAG+GGG 0.693669 1_3:+56098271 Msa0126950:three_prime_UTR
GCTGATGGTCTTGTAAACAG+AGG 0.706829 1_3:+56092378 Msa0126950:CDS
ATACGGTGCAGATAATCCAG+TGG 0.709165 1_3:-56092090 None:intergenic
TGATTGGAATAGGAGCAGGA+TGG 0.709742 1_3:-56094849 None:intergenic
ATGTGGAATCTGCATTGCTG+TGG 0.711848 1_3:+56098183 Msa0126950:CDS
GCACAACAGAAAGAATGTGG+AGG 0.713802 1_3:+56095065 Msa0126950:CDS
TCGTTTACAAAGTAGCAATG+CGG 0.715211 1_3:-56096762 None:intergenic
TCTGAAAGATGTTGGCAGAG+TGG 0.720915 1_3:+56091743 Msa0126950:five_prime_UTR
TTGTTAATGCAGGAACATGG+TGG 0.744612 1_3:+56092028 Msa0126950:intron
ATCAGATTATGGTCTCCCTG+AGG 0.756050 1_3:+56096941 Msa0126950:CDS
CAGCTGCCAAGGGATCAATG+CGG 0.837731 1_3:-56092359 None:intergenic
GATAGCAAAATTGATCAACG+AGG 0.841690 1_3:+56095276 Msa0126950:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GGTAATGCTCTGAAAGATGT+TGG + chr1_3:56091735-56091754 Msa0126950:five_prime_UTR 40.0%
TCTGAAAGATGTTGGCAGAG+TGG + chr1_3:56091743-56091762 Msa0126950:five_prime_UTR 45.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 56091734 56098943 56091734 ID=Msa0126950;Name=Msa0126950
chr1_3 mRNA 56091734 56098943 56091734 ID=Msa0126950-mRNA-1;Parent=Msa0126950;Name=Msa0126950-mRNA-1;_AED=0.17;_eAED=0.17;_QI=115|0.88|0.94|0.94|0.94|0.94|19|677|638
chr1_3 exon 56091734 56091788 56091734 ID=Msa0126950-mRNA-1:exon:17602;Parent=Msa0126950-mRNA-1
chr1_3 exon 56091887 56091954 56091887 ID=Msa0126950-mRNA-1:exon:17603;Parent=Msa0126950-mRNA-1
chr1_3 exon 56092040 56092142 56092040 ID=Msa0126950-mRNA-1:exon:17604;Parent=Msa0126950-mRNA-1
chr1_3 exon 56092318 56092520 56092318 ID=Msa0126950-mRNA-1:exon:17605;Parent=Msa0126950-mRNA-1
chr1_3 exon 56092844 56092940 56092844 ID=Msa0126950-mRNA-1:exon:17606;Parent=Msa0126950-mRNA-1
chr1_3 exon 56094429 56094500 56094429 ID=Msa0126950-mRNA-1:exon:17607;Parent=Msa0126950-mRNA-1
chr1_3 exon 56094613 56094689 56094613 ID=Msa0126950-mRNA-1:exon:17608;Parent=Msa0126950-mRNA-1
chr1_3 exon 56094789 56094905 56094789 ID=Msa0126950-mRNA-1:exon:17609;Parent=Msa0126950-mRNA-1
chr1_3 exon 56095002 56095086 56095002 ID=Msa0126950-mRNA-1:exon:17610;Parent=Msa0126950-mRNA-1
chr1_3 exon 56095172 56095339 56095172 ID=Msa0126950-mRNA-1:exon:17611;Parent=Msa0126950-mRNA-1
chr1_3 exon 56095455 56095544 56095455 ID=Msa0126950-mRNA-1:exon:17612;Parent=Msa0126950-mRNA-1
chr1_3 exon 56096502 56096612 56096502 ID=Msa0126950-mRNA-1:exon:17613;Parent=Msa0126950-mRNA-1
chr1_3 exon 56096708 56096794 56096708 ID=Msa0126950-mRNA-1:exon:17614;Parent=Msa0126950-mRNA-1
chr1_3 exon 56096900 56097027 56096900 ID=Msa0126950-mRNA-1:exon:17615;Parent=Msa0126950-mRNA-1
chr1_3 exon 56097139 56097221 56097139 ID=Msa0126950-mRNA-1:exon:17616;Parent=Msa0126950-mRNA-1
chr1_3 exon 56097433 56097538 56097433 ID=Msa0126950-mRNA-1:exon:17617;Parent=Msa0126950-mRNA-1
chr1_3 exon 56097645 56097791 56097645 ID=Msa0126950-mRNA-1:exon:17618;Parent=Msa0126950-mRNA-1
chr1_3 exon 56097944 56098064 56097944 ID=Msa0126950-mRNA-1:exon:17619;Parent=Msa0126950-mRNA-1
chr1_3 exon 56098153 56098943 56098153 ID=Msa0126950-mRNA-1:exon:17620;Parent=Msa0126950-mRNA-1
chr1_3 five_prime_UTR 56091734 56091788 56091734 ID=Msa0126950-mRNA-1:five_prime_utr;Parent=Msa0126950-mRNA-1
chr1_3 five_prime_UTR 56091887 56091946 56091887 ID=Msa0126950-mRNA-1:five_prime_utr;Parent=Msa0126950-mRNA-1
chr1_3 CDS 56091947 56091954 56091947 ID=Msa0126950-mRNA-1:cds;Parent=Msa0126950-mRNA-1
chr1_3 CDS 56092040 56092142 56092040 ID=Msa0126950-mRNA-1:cds;Parent=Msa0126950-mRNA-1
chr1_3 CDS 56092318 56092520 56092318 ID=Msa0126950-mRNA-1:cds;Parent=Msa0126950-mRNA-1
chr1_3 CDS 56092844 56092940 56092844 ID=Msa0126950-mRNA-1:cds;Parent=Msa0126950-mRNA-1
chr1_3 CDS 56094429 56094500 56094429 ID=Msa0126950-mRNA-1:cds;Parent=Msa0126950-mRNA-1
chr1_3 CDS 56094613 56094689 56094613 ID=Msa0126950-mRNA-1:cds;Parent=Msa0126950-mRNA-1
chr1_3 CDS 56094789 56094905 56094789 ID=Msa0126950-mRNA-1:cds;Parent=Msa0126950-mRNA-1
chr1_3 CDS 56095002 56095086 56095002 ID=Msa0126950-mRNA-1:cds;Parent=Msa0126950-mRNA-1
chr1_3 CDS 56095172 56095339 56095172 ID=Msa0126950-mRNA-1:cds;Parent=Msa0126950-mRNA-1
chr1_3 CDS 56095455 56095544 56095455 ID=Msa0126950-mRNA-1:cds;Parent=Msa0126950-mRNA-1
chr1_3 CDS 56096502 56096612 56096502 ID=Msa0126950-mRNA-1:cds;Parent=Msa0126950-mRNA-1
chr1_3 CDS 56096708 56096794 56096708 ID=Msa0126950-mRNA-1:cds;Parent=Msa0126950-mRNA-1
chr1_3 CDS 56096900 56097027 56096900 ID=Msa0126950-mRNA-1:cds;Parent=Msa0126950-mRNA-1
chr1_3 CDS 56097139 56097221 56097139 ID=Msa0126950-mRNA-1:cds;Parent=Msa0126950-mRNA-1
chr1_3 CDS 56097433 56097538 56097433 ID=Msa0126950-mRNA-1:cds;Parent=Msa0126950-mRNA-1
chr1_3 CDS 56097645 56097791 56097645 ID=Msa0126950-mRNA-1:cds;Parent=Msa0126950-mRNA-1
chr1_3 CDS 56097944 56098064 56097944 ID=Msa0126950-mRNA-1:cds;Parent=Msa0126950-mRNA-1
chr1_3 CDS 56098153 56098266 56098153 ID=Msa0126950-mRNA-1:cds;Parent=Msa0126950-mRNA-1
chr1_3 three_prime_UTR 56098267 56098943 56098267 ID=Msa0126950-mRNA-1:three_prime_utr;Parent=Msa0126950-mRNA-1
Gene Sequence

>Msa0126950

ATGGAATGGAACATGGTGGCTGCTGCTGCTTCATATTATAAAGCTACACTAAATGTAACCACTGGATTATCTGCACCGTATAACAACCTTGCTATAATATATAAGCAGCAGGGGAATTATGCAGATGCCATATCTTGCTACAATGAGGTCCTCCGCATTGATCCCTTGGCAGCTGATGGTCTTGTAAACAGAGGCAATACTTATAAGGAGATTGGTAGAGTCAGTGATGCTATCCAGGATTACATACGTGCGATTACCGTCAGGCCTACCATGGCTGAAGCTCATGCTAATTTGGCTTCTGCCTATAAGGATAGTGGACATGTAGAGGCGGCTGTAAAGAGCTACAGACAGGCATTGATTCTTCGTACAGACTTTCCTGAAGCAACTTGCAATCTTCTACATACACTTCAGTGTGTCTGTTGTTGGGAGGATCGTGATCAAATGTTCAAGGAAGTAGAAGGGATTATCAGAAGACAAATTAATATGTCAGTTCTTCCTAGTGTCCAGCCTTTCCATGCCATAGCATATCCACTTGACCCAATGCTTGCTCTGGAAATTAGCCGCAAATATGCTGCACACTGCTCTGTAATTGCATCTCGTTTCTCTCTACCACCATTTAGCCATCCTGCTCCTATTCCAATCAAGCAGGAGGGAGGTTATGAAAGACTAAGGATTGGGTATGTGAGTAGTGACTTTGGTAACCACCCATTGTCACATCTCATGGGATCTGTTTTCGGTATGCACAACAGAAAGAATGTGGAGGTATTCTGTTATGCCTTGAGTCCAAATGATGGTACTGAATGGAGACAGCGTATTCAATCTGAAACAGAGCACTTTGTGGATGTATCTGCCATGACATCAGATACGATAGCAAAATTGATCAACGAGGATAAGATTCAAATCCTTATCAACCTTAATGGTTATACAAAGTTTTATTTATGGATGATTGAATTCAGTTCTGATTTTGTGTTTAGTACTTTGATCCGTGAAGGGTATGTGAAATGGAGGAATGCTTCAGATGGTGCAAGAAATGAGATATTTGCTATGAAACCTGCACCAGTTCAAGTTTCATATATGGGATTTCCTGGCACAACCGGAGCTACATACATAGATTACTTGGTCACTGATGAGTTTGTTTCACCTCTTCAGTATGCACATATCTACTCTGAAAAGATTGTCCATCTCCCGCATTGCTACTTTGTAAACGATTATAAACAGAAAAATCAGGATGTGTTAGATCCAAACTGTCAGCCCAAACGATCAGATTATGGTCTCCCTGAGGATAAGTTTTTATTTGCATGCTTCAATCAACTTTACAAGATGGATCCTGAAATATTTAATACATGGTGCAATATACTTAAACGTGTACCCAACAGCGCGCTTTGGCTCTTGAAATTTCCTGCAGCAGGAGAAATGAGACTGAGGGCATATGCTGCTGCACAAGGGGTGCAACCTGATCAAATTATCTTCACAGATGTTGCAATGAAGGGTGAACACATTAGACGGAGTTCCTTGGCAGACTTATTTCTTGACACGCCTTTATGCAATGCACATACCACAGGAACAGATATATTATGGGCAGGTCTGCCTATGGTTACTCTGCCTCTTGAGAAAATGGCTACTAGGGTTGCTGGCTCACTCTGTATTTCCACTGGACTGGGGGAGGAGATGATTGTCAGCAGTATGAAAGAGTATGAAGATAGAGCTGTATCGCTTGCTCTGAATCGCCCAAAGCTTCAAGCTCTAACTGACAAGCTCAAATCTGTGCGTCTGACCTGTCCTCTCTTTGACACAAACCGATGGGTGAGAAATCTCGACAGAGCATATTTTAAAATGTGGAATCTGCATTGCTGTGGTCAACGGCCACAGCATTTTAAGGTCACTGAAAATGATAACGAATGTCCAGATGATAAATAG
Protein sequence

>Msa0126950

MEWNMVAAAASYYKATLNVTTGLSAPYNNLAIIYKQQGNYADAISCYNEVLRIDPLAADGLVNRGNTYKEIGRVSDAIQDYIRAITVRPTMAEAHANLASAYKDSGHVEAAVKSYRQALILRTDFPEATCNLLHTLQCVCCWEDRDQMFKEVEGIIRRQINMSVLPSVQPFHAIAYPLDPMLALEISRKYAAHCSVIASRFSLPPFSHPAPIPIKQEGGYERLRIGYVSSDFGNHPLSHLMGSVFGMHNRKNVEVFCYALSPNDGTEWRQRIQSETEHFVDVSAMTSDTIAKLINEDKIQILINLNGYTKFYLWMIEFSSDFVFSTLIREGYVKWRNASDGARNEIFAMKPAPVQVSYMGFPGTTGATYIDYLVTDEFVSPLQYAHIYSEKIVHLPHCYFVNDYKQKNQDVLDPNCQPKRSDYGLPEDKFLFACFNQLYKMDPEIFNTWCNILKRVPNSALWLLKFPAAGEMRLRAYAAAQGVQPDQIIFTDVAMKGEHIRRSSLADLFLDTPLCNAHTTGTDILWAGLPMVTLPLEKMATRVAGSLCISTGLGEEMIVSSMKEYEDRAVSLALNRPKLQALTDKLKSVRLTCPLFDTNRWVRNLDRAYFKMWNLHCCGQRPQHFKVTENDNECPDDK*