AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0127760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0127760 MtrunA17_Chr1g0210651 94.393 107 6 0 85 191 1 107 4.79e-72 213
Msa0127760 MtrunA17_Chr1g0210621 93.458 107 7 0 85 191 1 107 2.52e-69 206
Msa0127760 MtrunA17_Chr7g0266481 51.402 107 51 1 85 191 1 106 1.13e-33 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0127760 AT4G18590.1 58.879 107 43 1 85 191 1 106 1.04e-39 131
Msa0127760 AT3G52630.2 57.009 107 46 0 85 191 1 107 1.61e-38 128
Msa0127760 AT3G52630.1 57.009 107 46 0 85 191 1 107 1.61e-38 128

Find 60 sgRNAs with CRISPR-Local

Find 281 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATCTCAGATAACTGTAAA+AGG 0.220277 1_3:+57358331 Msa0127760:CDS
ATGCAGAAGTCTGTTTCTTT+AGG 0.240072 1_3:+57358775 Msa0127760:CDS
ATGAATTATGCTGAGGTTAT+TGG 0.271395 1_3:+57358376 Msa0127760:CDS
CATCTATGCAATCTCTAGTT+TGG 0.281812 1_3:+57358974 Msa0127760:three_prime_UTR
TGCAGAAGTCTGTTTCTTTA+GGG 0.290932 1_3:+57358776 Msa0127760:CDS
TTAGGGAGCTTGAAGATTGC+TGG 0.305055 1_3:+57358793 Msa0127760:exon
CAAGTTCTTCCCAATTTCAT+TGG 0.308622 1_3:+57358238 Msa0127760:CDS
GGCTGTCTTCAGATTTAGTT+TGG 0.316035 1_3:-57356685 None:intergenic
GTCAGCTACTGGATGTTATT+AGG 0.318836 1_3:+57358816 Msa0127760:three_prime_UTR
CAATGAAATTGGGAAGAACT+TGG 0.319382 1_3:-57358237 None:intergenic
AAAAGTAAGAACAGTGGTTC+AGG 0.370182 1_3:+57358264 Msa0127760:CDS
GCTGGAGCTCTGGGTCTTGA+AGG 0.383682 1_3:+57356800 Msa0127760:CDS
GAGGAGGCAAGCTGGAGCTC+TGG 0.385362 1_3:+57356790 Msa0127760:CDS
AAGATTGCTGGGTCAGCTAC+TGG 0.398877 1_3:+57358805 Msa0127760:three_prime_UTR
TGCATTCACCATTTGCTAGT+TGG 0.409283 1_3:-57358757 None:intergenic
ACTGACTTTGGTGCCACATT+TGG 0.420458 1_3:+57358439 Msa0127760:CDS
TTCCGTTCGTGTCTGCTCTT+CGG 0.444827 1_3:-57356706 None:intergenic
GTTTGTTGTTGGTAAGTCTC+TGG 0.454743 1_3:-57356739 None:intergenic
GCTGAAATATTGACTGACTT+TGG 0.464534 1_3:+57358427 Msa0127760:CDS
CATAATTCATGACAGGAACC+TGG 0.485773 1_3:-57358363 None:intergenic
TCTGATGGTGCGACAATCAC+GGG 0.488727 1_3:+57358295 Msa0127760:CDS
TAGGGAGCTTGAAGATTGCT+GGG 0.492019 1_3:+57358794 Msa0127760:exon
AGGAGGCAAGCTGGAGCTCT+GGG 0.495829 1_3:+57356791 Msa0127760:CDS
CGACGATGGAGGAGGCAAGC+TGG 0.501022 1_3:+57356782 Msa0127760:CDS
CTCCCAAACGCTTGCAGTGG+CGG 0.511182 1_3:+57356640 None:intergenic
CAGCTATGCCAACTAGCAAA+TGG 0.516075 1_3:+57358749 Msa0127760:CDS
ATTGCATAGATGTAAGTTCA+AGG 0.520489 1_3:-57358963 None:intergenic
AATCTCAGATAACTGTAAAA+GGG 0.520890 1_3:+57358332 Msa0127760:CDS
TTCTGATGGTGCGACAATCA+CGG 0.524842 1_3:+57358294 Msa0127760:CDS
CGACGGTTGCGTCGCCGTTG+TGG 0.529178 1_3:-57356875 None:intergenic
TGTAAAAGGGTTATCATCCC+AGG 0.534360 1_3:+57358345 Msa0127760:CDS
GGGTCTTGAAGGTGGAGACT+AGG 0.539111 1_3:+57356811 Msa0127760:CDS
AATGAAATTGGGAAGAACTT+GGG 0.544653 1_3:-57358236 None:intergenic
CAGCAAAACGACGATGGAGG+AGG 0.545240 1_3:+57356774 Msa0127760:CDS
ACCTCAGCATAATTCATGAC+AGG 0.554187 1_3:-57358370 None:intergenic
TGGGCATTGACAAATGCAGC+AGG 0.558969 1_3:-57358217 None:intergenic
GCTGTCCTTGTTCAATAAGA+TGG 0.561350 1_3:-57356853 None:intergenic
AGCCGAAGAGCAGACACGAA+CGG 0.567009 1_3:+57356704 Msa0127760:CDS
AGACGGCAGCAAAACGACGA+TGG 0.575010 1_3:+57356768 Msa0127760:CDS
GGAGCTCTGGGTCTTGAAGG+TGG 0.578145 1_3:+57356803 Msa0127760:CDS
GTCCTTGTTCAATAAGATGG+CGG 0.591646 1_3:-57356850 None:intergenic
ATAATTCATGACAGGAACCT+GGG 0.597968 1_3:-57358362 None:intergenic
ATAGTGCTTCTTACCAAATG+TGG 0.600089 1_3:-57358452 None:intergenic
AACCTCCCAAACGCTTGCAG+TGG 0.604858 1_3:+57356637 None:intergenic
TCCTGTCATGAATTATGCTG+AGG 0.605475 1_3:+57358369 Msa0127760:CDS
CACAACGGCGACGCAACCGT+CGG 0.609731 1_3:+57356876 Msa0127760:CDS
TGTTGTTGGTAAGTCTCTGG+CGG 0.617771 1_3:-57356736 None:intergenic
TTCTGCGAGGATCAGACATG+TGG 0.617829 1_3:+57358913 Msa0127760:three_prime_UTR
CAACAACAAACCTCAAAAGA+CGG 0.619004 1_3:+57356751 Msa0127760:CDS
TTGCATAGATGTAAGTTCAA+GGG 0.619501 1_3:-57358962 None:intergenic
GCAGACACGAACGGAAGTTG+AGG 0.620481 1_3:+57356713 Msa0127760:CDS
ACGTATATGTTTATTCTGCG+AGG 0.641771 1_3:+57358900 Msa0127760:three_prime_UTR
TTTGTGTAATCAAACTCCGA+CGG 0.642640 1_3:-57356892 None:intergenic
AACCGCCATCTTATTGAACA+AGG 0.651300 1_3:+57356848 Msa0127760:CDS
CGGCAGCAAAACGACGATGG+AGG 0.654016 1_3:+57356771 Msa0127760:CDS
AGTCAGTCAATATTTCAGCA+TGG 0.662550 1_3:-57358423 None:intergenic
GTTCAGGTGAATCATTCTGA+TGG 0.665709 1_3:+57358280 Msa0127760:CDS
TTGAAGGTGGAGACTAGGAA+CGG 0.667962 1_3:+57356816 Msa0127760:CDS
TTGAACAAGGACAGCCACAA+CGG 0.693783 1_3:+57356861 Msa0127760:CDS
AAGGTGGAGACTAGGAACGG+AGG 0.716438 1_3:+57356819 Msa0127760:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAGTAAATGTATGTTAA+TGG - chr1_3:57357016-57357035 None:intergenic 15.0%
!!! CATTACTAATCTTTTTTTTA+AGG + chr1_3:57357122-57357141 Msa0127760:intron 15.0%
!! ACTTATTATTATGAATGAAT+TGG + chr1_3:57357986-57358005 Msa0127760:intron 15.0%
!! CTTATTATTATGAATGAATT+GGG + chr1_3:57357987-57358006 Msa0127760:intron 15.0%
!!! TTTTTTTTTTTGAAAGGAAT+TGG + chr1_3:57358018-57358037 Msa0127760:intron 15.0%
!!! TTTTTTTTTTGAAAGGAATT+GGG + chr1_3:57358019-57358038 Msa0127760:intron 15.0%
!!! TGAATCTTTTTTTATATTGT+TGG + chr1_3:57358152-57358171 Msa0127760:intron 15.0%
!!! AGAAATTTAAAACACAAATT+AGG - chr1_3:57358659-57358678 None:intergenic 15.0%
!! AATAAGTAAATGTATGTTAA+TGG - chr1_3:57357016-57357035 None:intergenic 15.0%
!!! CATTACTAATCTTTTTTTTA+AGG + chr1_3:57357122-57357141 Msa0127760:intron 15.0%
!! ACTTATTATTATGAATGAAT+TGG + chr1_3:57357986-57358005 Msa0127760:intron 15.0%
!! CTTATTATTATGAATGAATT+GGG + chr1_3:57357987-57358006 Msa0127760:intron 15.0%
!!! TTTTTTTTTTTGAAAGGAAT+TGG + chr1_3:57358018-57358037 Msa0127760:intron 15.0%
!!! TTTTTTTTTTGAAAGGAATT+GGG + chr1_3:57358019-57358038 Msa0127760:intron 15.0%
!!! TGAATCTTTTTTTATATTGT+TGG + chr1_3:57358152-57358171 Msa0127760:intron 15.0%
!!! AGAAATTTAAAACACAAATT+AGG - chr1_3:57358659-57358678 None:intergenic 15.0%
!!! ATTAGTAATGTTGATTGTAA+TGG - chr1_3:57357112-57357131 None:intergenic 20.0%
!!! GGTAAGATTTTATTAAATAG+TGG + chr1_3:57357192-57357211 Msa0127760:intron 20.0%
!!! TCTACTTTATCTTACTATTT+TGG - chr1_3:57357567-57357586 None:intergenic 20.0%
!! AAGGTTATAAATAATAGAAG+CGG - chr1_3:57357666-57357685 None:intergenic 20.0%
!! ATAAGTGCATAAAAAAAAAG+GGG - chr1_3:57357892-57357911 None:intergenic 20.0%
!! GATAAGTGCATAAAAAAAAA+GGG - chr1_3:57357893-57357912 None:intergenic 20.0%
!! TGATAAGTGCATAAAAAAAA+AGG - chr1_3:57357894-57357913 None:intergenic 20.0%
!!! ACTTTTTTTCCAATGAAATT+GGG - chr1_3:57358250-57358269 None:intergenic 20.0%
!!! TACTTTTTTTCCAATGAAAT+TGG - chr1_3:57358251-57358270 None:intergenic 20.0%
!! TTAAATCTCCAAATTAGAAT+AGG + chr1_3:57358629-57358648 Msa0127760:intron 20.0%
!!! ATTTCTTTTGTTACAATGTT+TGG + chr1_3:57358673-57358692 Msa0127760:intron 20.0%
!!! TACTTAAGTTGATTTTGATT+TGG + chr1_3:57358697-57358716 Msa0127760:intron 20.0%
!!! ATTAGTAATGTTGATTGTAA+TGG - chr1_3:57357112-57357131 None:intergenic 20.0%
!!! GGTAAGATTTTATTAAATAG+TGG + chr1_3:57357192-57357211 Msa0127760:intron 20.0%
!!! TCTACTTTATCTTACTATTT+TGG - chr1_3:57357567-57357586 None:intergenic 20.0%
!! AAGGTTATAAATAATAGAAG+CGG - chr1_3:57357666-57357685 None:intergenic 20.0%
!! ATAAGTGCATAAAAAAAAAG+GGG - chr1_3:57357892-57357911 None:intergenic 20.0%
!! GATAAGTGCATAAAAAAAAA+GGG - chr1_3:57357893-57357912 None:intergenic 20.0%
!! TGATAAGTGCATAAAAAAAA+AGG - chr1_3:57357894-57357913 None:intergenic 20.0%
!!! ACTTTTTTTCCAATGAAATT+GGG - chr1_3:57358250-57358269 None:intergenic 20.0%
!!! TACTTTTTTTCCAATGAAAT+TGG - chr1_3:57358251-57358270 None:intergenic 20.0%
!! TTAAATCTCCAAATTAGAAT+AGG + chr1_3:57358629-57358648 Msa0127760:intron 20.0%
!!! ATTTCTTTTGTTACAATGTT+TGG + chr1_3:57358673-57358692 Msa0127760:intron 20.0%
!!! TACTTAAGTTGATTTTGATT+TGG + chr1_3:57358697-57358716 Msa0127760:intron 20.0%
!! CAAGACAATTTTTCTACTTT+TGG + chr1_3:57357254-57357273 Msa0127760:intron 25.0%
! TTACCTGGGTATTAAAAAAA+GGG - chr1_3:57357318-57357337 None:intergenic 25.0%
! TTTACCTGGGTATTAAAAAA+AGG - chr1_3:57357319-57357338 None:intergenic 25.0%
! TTCTTATTGGTCAAAAACAA+TGG - chr1_3:57357504-57357523 None:intergenic 25.0%
!! ATCAATTTTGTTCTTTATGG+CGG - chr1_3:57357633-57357652 None:intergenic 25.0%
!! ACGATCAATTTTGTTCTTTA+TGG - chr1_3:57357636-57357655 None:intergenic 25.0%
! AGGTTATAAATAATAGAAGC+GGG - chr1_3:57357665-57357684 None:intergenic 25.0%
!!! GAAACTTTTGTTTTAGATCT+TGG + chr1_3:57358058-57358077 Msa0127760:intron 25.0%
! TAATCAACTGAAATTTCTGA+TGG - chr1_3:57358103-57358122 None:intergenic 25.0%
! AATCTCAGATAACTGTAAAA+GGG + chr1_3:57358332-57358351 Msa0127760:CDS 25.0%
!!! TTTCTTACTTGTTTTCGTTA+TGG + chr1_3:57358493-57358512 Msa0127760:intron 25.0%
!!! ATGTTTTAGCATTTTATGAC+AGG + chr1_3:57358868-57358887 Msa0127760:three_prime_UTR 25.0%
!! CAAGACAATTTTTCTACTTT+TGG + chr1_3:57357254-57357273 Msa0127760:intron 25.0%
! TTACCTGGGTATTAAAAAAA+GGG - chr1_3:57357318-57357337 None:intergenic 25.0%
! TTTACCTGGGTATTAAAAAA+AGG - chr1_3:57357319-57357338 None:intergenic 25.0%
! TTCTTATTGGTCAAAAACAA+TGG - chr1_3:57357504-57357523 None:intergenic 25.0%
!! ATCAATTTTGTTCTTTATGG+CGG - chr1_3:57357633-57357652 None:intergenic 25.0%
!! ACGATCAATTTTGTTCTTTA+TGG - chr1_3:57357636-57357655 None:intergenic 25.0%
! AGGTTATAAATAATAGAAGC+GGG - chr1_3:57357665-57357684 None:intergenic 25.0%
!!! GAAACTTTTGTTTTAGATCT+TGG + chr1_3:57358058-57358077 Msa0127760:intron 25.0%
! TAATCAACTGAAATTTCTGA+TGG - chr1_3:57358103-57358122 None:intergenic 25.0%
! AATCTCAGATAACTGTAAAA+GGG + chr1_3:57358332-57358351 Msa0127760:CDS 25.0%
!!! TTTCTTACTTGTTTTCGTTA+TGG + chr1_3:57358493-57358512 Msa0127760:intron 25.0%
!!! ATGTTTTAGCATTTTATGAC+AGG + chr1_3:57358868-57358887 Msa0127760:three_prime_UTR 25.0%
!!! AGAGAACACTTATTTTGAGA+CGG - chr1_3:57356971-57356990 None:intergenic 30.0%
ATTGATTAAGCAGCTAAAAG+AGG + chr1_3:57357171-57357190 Msa0127760:intron 30.0%
AGCTAGTAATGCATAAGAAA+CGG + chr1_3:57357368-57357387 Msa0127760:intron 30.0%
GTGCAAAAGTTGTACAAAAA+TGG - chr1_3:57357424-57357443 None:intergenic 30.0%
AAAACAATGGAGTGAGAAAA+AGG - chr1_3:57357491-57357510 None:intergenic 30.0%
! CTCTCTTCTCATTTTCTTAT+TGG - chr1_3:57357517-57357536 None:intergenic 30.0%
AGATAAAGTAGATTTGCAGT+AGG + chr1_3:57357575-57357594 Msa0127760:intron 30.0%
GATAAAGTAGATTTGCAGTA+GGG + chr1_3:57357576-57357595 Msa0127760:intron 30.0%
! TCAATTTTGTTCTTTATGGC+GGG - chr1_3:57357632-57357651 None:intergenic 30.0%
! AATTACGCAAACGCATTTTA+GGG + chr1_3:57357714-57357733 Msa0127760:intron 30.0%
TCTTCCATTCATTCATTCTT+AGG + chr1_3:57357741-57357760 Msa0127760:intron 30.0%
CTTCCATTCATTCATTCTTA+GGG + chr1_3:57357742-57357761 Msa0127760:intron 30.0%
AAACCCTAAGAATGAATGAA+TGG - chr1_3:57357748-57357767 None:intergenic 30.0%
TCCGAAACAACAATTTCATA+CGG + chr1_3:57357780-57357799 Msa0127760:intron 30.0%
!! ACCGTATGAAATTGTTGTTT+CGG - chr1_3:57357784-57357803 None:intergenic 30.0%
ATCACTTGAAACAAAAGAGA+TGG - chr1_3:57357840-57357859 None:intergenic 30.0%
CATAAAAAAAAAGGGGAATC+TGG - chr1_3:57357885-57357904 None:intergenic 30.0%
AATGAAATTGGGAAGAACTT+GGG - chr1_3:57358239-57358258 None:intergenic 30.0%
TGGAAAAAAAGTAAGAACAG+TGG + chr1_3:57358258-57358277 Msa0127760:CDS 30.0%
GAATCTCAGATAACTGTAAA+AGG + chr1_3:57358331-57358350 Msa0127760:CDS 30.0%
ATGAATTATGCTGAGGTTAT+TGG + chr1_3:57358376-57358395 Msa0127760:CDS 30.0%
GAAAACTGTCACATAGTTAA+TGG + chr1_3:57358580-57358599 Msa0127760:intron 30.0%
TTGCATAGATGTAAGTTCAA+GGG - chr1_3:57358965-57358984 None:intergenic 30.0%
ATTGCATAGATGTAAGTTCA+AGG - chr1_3:57358966-57358985 None:intergenic 30.0%
!!! AGAGAACACTTATTTTGAGA+CGG - chr1_3:57356971-57356990 None:intergenic 30.0%
ATTGATTAAGCAGCTAAAAG+AGG + chr1_3:57357171-57357190 Msa0127760:intron 30.0%
AGCTAGTAATGCATAAGAAA+CGG + chr1_3:57357368-57357387 Msa0127760:intron 30.0%
GTGCAAAAGTTGTACAAAAA+TGG - chr1_3:57357424-57357443 None:intergenic 30.0%
AAAACAATGGAGTGAGAAAA+AGG - chr1_3:57357491-57357510 None:intergenic 30.0%
! CTCTCTTCTCATTTTCTTAT+TGG - chr1_3:57357517-57357536 None:intergenic 30.0%
AGATAAAGTAGATTTGCAGT+AGG + chr1_3:57357575-57357594 Msa0127760:intron 30.0%
GATAAAGTAGATTTGCAGTA+GGG + chr1_3:57357576-57357595 Msa0127760:intron 30.0%
! TCAATTTTGTTCTTTATGGC+GGG - chr1_3:57357632-57357651 None:intergenic 30.0%
! AATTACGCAAACGCATTTTA+GGG + chr1_3:57357714-57357733 Msa0127760:intron 30.0%
TCTTCCATTCATTCATTCTT+AGG + chr1_3:57357741-57357760 Msa0127760:intron 30.0%
CTTCCATTCATTCATTCTTA+GGG + chr1_3:57357742-57357761 Msa0127760:intron 30.0%
AAACCCTAAGAATGAATGAA+TGG - chr1_3:57357748-57357767 None:intergenic 30.0%
TCCGAAACAACAATTTCATA+CGG + chr1_3:57357780-57357799 Msa0127760:intron 30.0%
!! ACCGTATGAAATTGTTGTTT+CGG - chr1_3:57357784-57357803 None:intergenic 30.0%
ATCACTTGAAACAAAAGAGA+TGG - chr1_3:57357840-57357859 None:intergenic 30.0%
CATAAAAAAAAAGGGGAATC+TGG - chr1_3:57357885-57357904 None:intergenic 30.0%
AATGAAATTGGGAAGAACTT+GGG - chr1_3:57358239-57358258 None:intergenic 30.0%
TGGAAAAAAAGTAAGAACAG+TGG + chr1_3:57358258-57358277 Msa0127760:CDS 30.0%
GAATCTCAGATAACTGTAAA+AGG + chr1_3:57358331-57358350 Msa0127760:CDS 30.0%
ATGAATTATGCTGAGGTTAT+TGG + chr1_3:57358376-57358395 Msa0127760:CDS 30.0%
GAAAACTGTCACATAGTTAA+TGG + chr1_3:57358580-57358599 Msa0127760:intron 30.0%
TTGCATAGATGTAAGTTCAA+GGG - chr1_3:57358965-57358984 None:intergenic 30.0%
ATTGCATAGATGTAAGTTCA+AGG - chr1_3:57358966-57358985 None:intergenic 30.0%
CAACAACAAACCTCAAAAGA+CGG + chr1_3:57356751-57356770 Msa0127760:CDS 35.0%
TTTGTGTAATCAAACTCCGA+CGG - chr1_3:57356895-57356914 None:intergenic 35.0%
GAGACGGATGAAGTATAATT+AGG - chr1_3:57356955-57356974 None:intergenic 35.0%
TGGATTTCTTAACCAGTGTT+CGG + chr1_3:57357274-57357293 Msa0127760:intron 35.0%
!!! AGACCCTTTTTTTAATACCC+AGG + chr1_3:57357312-57357331 Msa0127760:intron 35.0%
TACCCAGGTAAAACTCAAAA+TGG + chr1_3:57357327-57357346 Msa0127760:intron 35.0%
!!! GTCCATTTTGAGTTTTACCT+GGG - chr1_3:57357332-57357351 None:intergenic 35.0%
!!! TGTCCATTTTGAGTTTTACC+TGG - chr1_3:57357333-57357352 None:intergenic 35.0%
!! TACTAGCTCTGTCTTAAACT+AGG - chr1_3:57357356-57357375 None:intergenic 35.0%
CTAAAATGCGTTTGCGTAAT+TGG - chr1_3:57357715-57357734 None:intergenic 35.0%
! CAATTACGCAAACGCATTTT+AGG + chr1_3:57357713-57357732 Msa0127760:intron 35.0%
!! GTATGAAATTGTTGTTTCGG+AGG - chr1_3:57357781-57357800 None:intergenic 35.0%
AAAAAAAAAGGGGAATCTGG+TGG - chr1_3:57357882-57357901 None:intergenic 35.0%
TATGCGAAACAAAATCTGTG+AGG - chr1_3:57357931-57357950 None:intergenic 35.0%
TTCTACTCTGTTTCAGATCA+TGG + chr1_3:57358183-57358202 Msa0127760:intron 35.0%
CAATGAAATTGGGAAGAACT+TGG - chr1_3:57358240-57358259 None:intergenic 35.0%
CAAGTTCTTCCCAATTTCAT+TGG + chr1_3:57358238-57358257 Msa0127760:CDS 35.0%
AAAAGTAAGAACAGTGGTTC+AGG + chr1_3:57358264-57358283 Msa0127760:CDS 35.0%
ATAATTCATGACAGGAACCT+GGG - chr1_3:57358365-57358384 None:intergenic 35.0%
AGTCAGTCAATATTTCAGCA+TGG - chr1_3:57358426-57358445 None:intergenic 35.0%
! GCTGAAATATTGACTGACTT+TGG + chr1_3:57358427-57358446 Msa0127760:CDS 35.0%
ATAGTGCTTCTTACCAAATG+TGG - chr1_3:57358455-57358474 None:intergenic 35.0%
TAGGAGAGCCTATTCTAATT+TGG - chr1_3:57358640-57358659 None:intergenic 35.0%
! ATGCAGAAGTCTGTTTCTTT+AGG + chr1_3:57358775-57358794 Msa0127760:CDS 35.0%
! TGCAGAAGTCTGTTTCTTTA+GGG + chr1_3:57358776-57358795 Msa0127760:CDS 35.0%
ACGTATATGTTTATTCTGCG+AGG + chr1_3:57358900-57358919 Msa0127760:three_prime_UTR 35.0%
CATCTATGCAATCTCTAGTT+TGG + chr1_3:57358974-57358993 Msa0127760:three_prime_UTR 35.0%
CAACAACAAACCTCAAAAGA+CGG + chr1_3:57356751-57356770 Msa0127760:CDS 35.0%
TTTGTGTAATCAAACTCCGA+CGG - chr1_3:57356895-57356914 None:intergenic 35.0%
GAGACGGATGAAGTATAATT+AGG - chr1_3:57356955-57356974 None:intergenic 35.0%
TGGATTTCTTAACCAGTGTT+CGG + chr1_3:57357274-57357293 Msa0127760:intron 35.0%
!!! AGACCCTTTTTTTAATACCC+AGG + chr1_3:57357312-57357331 Msa0127760:intron 35.0%
TACCCAGGTAAAACTCAAAA+TGG + chr1_3:57357327-57357346 Msa0127760:intron 35.0%
!!! GTCCATTTTGAGTTTTACCT+GGG - chr1_3:57357332-57357351 None:intergenic 35.0%
!!! TGTCCATTTTGAGTTTTACC+TGG - chr1_3:57357333-57357352 None:intergenic 35.0%
!! TACTAGCTCTGTCTTAAACT+AGG - chr1_3:57357356-57357375 None:intergenic 35.0%
CTAAAATGCGTTTGCGTAAT+TGG - chr1_3:57357715-57357734 None:intergenic 35.0%
! CAATTACGCAAACGCATTTT+AGG + chr1_3:57357713-57357732 Msa0127760:intron 35.0%
!! GTATGAAATTGTTGTTTCGG+AGG - chr1_3:57357781-57357800 None:intergenic 35.0%
AAAAAAAAAGGGGAATCTGG+TGG - chr1_3:57357882-57357901 None:intergenic 35.0%
TATGCGAAACAAAATCTGTG+AGG - chr1_3:57357931-57357950 None:intergenic 35.0%
TTCTACTCTGTTTCAGATCA+TGG + chr1_3:57358183-57358202 Msa0127760:intron 35.0%
CAATGAAATTGGGAAGAACT+TGG - chr1_3:57358240-57358259 None:intergenic 35.0%
CAAGTTCTTCCCAATTTCAT+TGG + chr1_3:57358238-57358257 Msa0127760:CDS 35.0%
AAAAGTAAGAACAGTGGTTC+AGG + chr1_3:57358264-57358283 Msa0127760:CDS 35.0%
ATAATTCATGACAGGAACCT+GGG - chr1_3:57358365-57358384 None:intergenic 35.0%
AGTCAGTCAATATTTCAGCA+TGG - chr1_3:57358426-57358445 None:intergenic 35.0%
! GCTGAAATATTGACTGACTT+TGG + chr1_3:57358427-57358446 Msa0127760:CDS 35.0%
ATAGTGCTTCTTACCAAATG+TGG - chr1_3:57358455-57358474 None:intergenic 35.0%
TAGGAGAGCCTATTCTAATT+TGG - chr1_3:57358640-57358659 None:intergenic 35.0%
! ATGCAGAAGTCTGTTTCTTT+AGG + chr1_3:57358775-57358794 Msa0127760:CDS 35.0%
! TGCAGAAGTCTGTTTCTTTA+GGG + chr1_3:57358776-57358795 Msa0127760:CDS 35.0%
ACGTATATGTTTATTCTGCG+AGG + chr1_3:57358900-57358919 Msa0127760:three_prime_UTR 35.0%
CATCTATGCAATCTCTAGTT+TGG + chr1_3:57358974-57358993 Msa0127760:three_prime_UTR 35.0%
GGCTGTCTTCAGATTTAGTT+TGG - chr1_3:57356688-57356707 None:intergenic 40.0%
!! GTTTGTTGTTGGTAAGTCTC+TGG - chr1_3:57356742-57356761 None:intergenic 40.0%
!!! CGTCTTTTGAGGTTTGTTGT+TGG - chr1_3:57356753-57356772 None:intergenic 40.0%
AACCGCCATCTTATTGAACA+AGG + chr1_3:57356848-57356867 Msa0127760:CDS 40.0%
GTCCTTGTTCAATAAGATGG+CGG - chr1_3:57356853-57356872 None:intergenic 40.0%
GCTGTCCTTGTTCAATAAGA+TGG - chr1_3:57356856-57356875 None:intergenic 40.0%
TAGCTGCTTAATCAATGCCT+CGG - chr1_3:57357167-57357186 None:intergenic 40.0%
GGATTTCTTAACCAGTGTTC+GGG + chr1_3:57357275-57357294 Msa0127760:intron 40.0%
GATTTCTTAACCAGTGTTCG+GGG + chr1_3:57357276-57357295 Msa0127760:intron 40.0%
ATTTCTTAACCAGTGTTCGG+GGG + chr1_3:57357277-57357296 Msa0127760:intron 40.0%
GATTTGCAGTAGGGAGAAAA+TGG + chr1_3:57357585-57357604 Msa0127760:intron 40.0%
GTTCAGGTGAATCATTCTGA+TGG + chr1_3:57358280-57358299 Msa0127760:CDS 40.0%
TGTAAAAGGGTTATCATCCC+AGG + chr1_3:57358345-57358364 Msa0127760:CDS 40.0%
CATAATTCATGACAGGAACC+TGG - chr1_3:57358366-57358385 None:intergenic 40.0%
TCCTGTCATGAATTATGCTG+AGG + chr1_3:57358369-57358388 Msa0127760:CDS 40.0%
ACCTCAGCATAATTCATGAC+AGG - chr1_3:57358373-57358392 None:intergenic 40.0%
! TGCATTCACCATTTGCTAGT+TGG - chr1_3:57358760-57358779 None:intergenic 40.0%
GTCAGCTACTGGATGTTATT+AGG + chr1_3:57358816-57358835 Msa0127760:three_prime_UTR 40.0%
GGCTGTCTTCAGATTTAGTT+TGG - chr1_3:57356688-57356707 None:intergenic 40.0%
!! GTTTGTTGTTGGTAAGTCTC+TGG - chr1_3:57356742-57356761 None:intergenic 40.0%
!!! CGTCTTTTGAGGTTTGTTGT+TGG - chr1_3:57356753-57356772 None:intergenic 40.0%
AACCGCCATCTTATTGAACA+AGG + chr1_3:57356848-57356867 Msa0127760:CDS 40.0%
GTCCTTGTTCAATAAGATGG+CGG - chr1_3:57356853-57356872 None:intergenic 40.0%
GCTGTCCTTGTTCAATAAGA+TGG - chr1_3:57356856-57356875 None:intergenic 40.0%
TAGCTGCTTAATCAATGCCT+CGG - chr1_3:57357167-57357186 None:intergenic 40.0%
GGATTTCTTAACCAGTGTTC+GGG + chr1_3:57357275-57357294 Msa0127760:intron 40.0%
GATTTCTTAACCAGTGTTCG+GGG + chr1_3:57357276-57357295 Msa0127760:intron 40.0%
ATTTCTTAACCAGTGTTCGG+GGG + chr1_3:57357277-57357296 Msa0127760:intron 40.0%
GATTTGCAGTAGGGAGAAAA+TGG + chr1_3:57357585-57357604 Msa0127760:intron 40.0%
GTTCAGGTGAATCATTCTGA+TGG + chr1_3:57358280-57358299 Msa0127760:CDS 40.0%
TGTAAAAGGGTTATCATCCC+AGG + chr1_3:57358345-57358364 Msa0127760:CDS 40.0%
CATAATTCATGACAGGAACC+TGG - chr1_3:57358366-57358385 None:intergenic 40.0%
TCCTGTCATGAATTATGCTG+AGG + chr1_3:57358369-57358388 Msa0127760:CDS 40.0%
ACCTCAGCATAATTCATGAC+AGG - chr1_3:57358373-57358392 None:intergenic 40.0%
! TGCATTCACCATTTGCTAGT+TGG - chr1_3:57358760-57358779 None:intergenic 40.0%
GTCAGCTACTGGATGTTATT+AGG + chr1_3:57358816-57358835 Msa0127760:three_prime_UTR 40.0%
! TGTTGTTGGTAAGTCTCTGG+CGG - chr1_3:57356739-57356758 None:intergenic 45.0%
TTGAAGGTGGAGACTAGGAA+CGG + chr1_3:57356816-57356835 Msa0127760:CDS 45.0%
TTGAACAAGGACAGCCACAA+CGG + chr1_3:57356861-57356880 Msa0127760:CDS 45.0%
AGCTGCTTAATCAATGCCTC+GGG - chr1_3:57357166-57357185 None:intergenic 45.0%
! TTCTGATGGTGCGACAATCA+CGG + chr1_3:57358294-57358313 Msa0127760:CDS 45.0%
!! ACTGACTTTGGTGCCACATT+TGG + chr1_3:57358439-57358458 Msa0127760:CDS 45.0%
CAGCTATGCCAACTAGCAAA+TGG + chr1_3:57358749-57358768 Msa0127760:CDS 45.0%
TTAGGGAGCTTGAAGATTGC+TGG + chr1_3:57358793-57358812 Msa0127760:exon 45.0%
TAGGGAGCTTGAAGATTGCT+GGG + chr1_3:57358794-57358813 Msa0127760:exon 45.0%
! TGTTGTTGGTAAGTCTCTGG+CGG - chr1_3:57356739-57356758 None:intergenic 45.0%
TTGAAGGTGGAGACTAGGAA+CGG + chr1_3:57356816-57356835 Msa0127760:CDS 45.0%
TTGAACAAGGACAGCCACAA+CGG + chr1_3:57356861-57356880 Msa0127760:CDS 45.0%
AGCTGCTTAATCAATGCCTC+GGG - chr1_3:57357166-57357185 None:intergenic 45.0%
! TTCTGATGGTGCGACAATCA+CGG + chr1_3:57358294-57358313 Msa0127760:CDS 45.0%
!! ACTGACTTTGGTGCCACATT+TGG + chr1_3:57358439-57358458 Msa0127760:CDS 45.0%
CAGCTATGCCAACTAGCAAA+TGG + chr1_3:57358749-57358768 Msa0127760:CDS 45.0%
TTAGGGAGCTTGAAGATTGC+TGG + chr1_3:57358793-57358812 Msa0127760:exon 45.0%
TAGGGAGCTTGAAGATTGCT+GGG + chr1_3:57358794-57358813 Msa0127760:exon 45.0%
!! TCAAAATAAAAATAAAAAAA+AGG - chr1_3:57357967-57357986 None:intergenic 5.0%
!!! AAATTTTTTTTTTTTTTGAA+AGG + chr1_3:57358012-57358031 Msa0127760:intron 5.0%
!! TCAAAATAAAAATAAAAAAA+AGG - chr1_3:57357967-57357986 None:intergenic 5.0%
!!! AAATTTTTTTTTTTTTTGAA+AGG + chr1_3:57358012-57358031 Msa0127760:intron 5.0%
TTCCGTTCGTGTCTGCTCTT+CGG - chr1_3:57356709-57356728 None:intergenic 50.0%
! CGTTTTGCTGCCGTCTTTTG+AGG - chr1_3:57356764-57356783 None:intergenic 50.0%
CTGCTTAATCAATGCCTCGG+GGG - chr1_3:57357164-57357183 None:intergenic 50.0%
GCTGCTTAATCAATGCCTCG+GGG - chr1_3:57357165-57357184 None:intergenic 50.0%
!! TGGGCATTGACAAATGCAGC+AGG - chr1_3:57358220-57358239 None:intergenic 50.0%
! TCTGATGGTGCGACAATCAC+GGG + chr1_3:57358295-57358314 Msa0127760:CDS 50.0%
AAGATTGCTGGGTCAGCTAC+TGG + chr1_3:57358805-57358824 Msa0127760:three_prime_UTR 50.0%
TTCTGCGAGGATCAGACATG+TGG + chr1_3:57358913-57358932 Msa0127760:three_prime_UTR 50.0%
TTCCGTTCGTGTCTGCTCTT+CGG - chr1_3:57356709-57356728 None:intergenic 50.0%
! CGTTTTGCTGCCGTCTTTTG+AGG - chr1_3:57356764-57356783 None:intergenic 50.0%
CTGCTTAATCAATGCCTCGG+GGG - chr1_3:57357164-57357183 None:intergenic 50.0%
GCTGCTTAATCAATGCCTCG+GGG - chr1_3:57357165-57357184 None:intergenic 50.0%
!! TGGGCATTGACAAATGCAGC+AGG - chr1_3:57358220-57358239 None:intergenic 50.0%
! TCTGATGGTGCGACAATCAC+GGG + chr1_3:57358295-57358314 Msa0127760:CDS 50.0%
AAGATTGCTGGGTCAGCTAC+TGG + chr1_3:57358805-57358824 Msa0127760:three_prime_UTR 50.0%
TTCTGCGAGGATCAGACATG+TGG + chr1_3:57358913-57358932 Msa0127760:three_prime_UTR 50.0%
AGCCGAAGAGCAGACACGAA+CGG + chr1_3:57356704-57356723 Msa0127760:CDS 55.0%
GCAGACACGAACGGAAGTTG+AGG + chr1_3:57356713-57356732 Msa0127760:CDS 55.0%
AGACGGCAGCAAAACGACGA+TGG + chr1_3:57356768-57356787 Msa0127760:CDS 55.0%
CAGCAAAACGACGATGGAGG+AGG + chr1_3:57356774-57356793 Msa0127760:CDS 55.0%
GGGTCTTGAAGGTGGAGACT+AGG + chr1_3:57356811-57356830 Msa0127760:CDS 55.0%
AAGGTGGAGACTAGGAACGG+AGG + chr1_3:57356819-57356838 Msa0127760:CDS 55.0%
AATGTTAACCAGTGCCCCCG+AGG + chr1_3:57357147-57357166 Msa0127760:intron 55.0%
!! AGAGCGCTGCAACACGGAAA+AGG - chr1_3:57357685-57357704 None:intergenic 55.0%
!! GTTGAGAGAGCGCTGCAACA+CGG - chr1_3:57357691-57357710 None:intergenic 55.0%
AGCCGAAGAGCAGACACGAA+CGG + chr1_3:57356704-57356723 Msa0127760:CDS 55.0%
GCAGACACGAACGGAAGTTG+AGG + chr1_3:57356713-57356732 Msa0127760:CDS 55.0%
AGACGGCAGCAAAACGACGA+TGG + chr1_3:57356768-57356787 Msa0127760:CDS 55.0%
CAGCAAAACGACGATGGAGG+AGG + chr1_3:57356774-57356793 Msa0127760:CDS 55.0%
GGGTCTTGAAGGTGGAGACT+AGG + chr1_3:57356811-57356830 Msa0127760:CDS 55.0%
AAGGTGGAGACTAGGAACGG+AGG + chr1_3:57356819-57356838 Msa0127760:CDS 55.0%
AATGTTAACCAGTGCCCCCG+AGG + chr1_3:57357147-57357166 Msa0127760:intron 55.0%
!! AGAGCGCTGCAACACGGAAA+AGG - chr1_3:57357685-57357704 None:intergenic 55.0%
!! GTTGAGAGAGCGCTGCAACA+CGG - chr1_3:57357691-57357710 None:intergenic 55.0%
CGGCAGCAAAACGACGATGG+AGG + chr1_3:57356771-57356790 Msa0127760:CDS 60.0%
AGGAGGCAAGCTGGAGCTCT+GGG + chr1_3:57356791-57356810 Msa0127760:CDS 60.0%
!! GCTGGAGCTCTGGGTCTTGA+AGG + chr1_3:57356800-57356819 Msa0127760:CDS 60.0%
!! GGAGCTCTGGGTCTTGAAGG+TGG + chr1_3:57356803-57356822 Msa0127760:CDS 60.0%
! AATCAATGCCTCGGGGGCAC+TGG - chr1_3:57357158-57357177 None:intergenic 60.0%
! TAACCAGTGTTCGGGGGCAC+TGG + chr1_3:57357283-57357302 Msa0127760:intron 60.0%
TAACCAGTGCCCCCGAACAC+TGG - chr1_3:57357289-57357308 None:intergenic 60.0%
CGGCAGCAAAACGACGATGG+AGG + chr1_3:57356771-57356790 Msa0127760:CDS 60.0%
AGGAGGCAAGCTGGAGCTCT+GGG + chr1_3:57356791-57356810 Msa0127760:CDS 60.0%
!! GCTGGAGCTCTGGGTCTTGA+AGG + chr1_3:57356800-57356819 Msa0127760:CDS 60.0%
!! GGAGCTCTGGGTCTTGAAGG+TGG + chr1_3:57356803-57356822 Msa0127760:CDS 60.0%
! AATCAATGCCTCGGGGGCAC+TGG - chr1_3:57357158-57357177 None:intergenic 60.0%
! TAACCAGTGTTCGGGGGCAC+TGG + chr1_3:57357283-57357302 Msa0127760:intron 60.0%
TAACCAGTGCCCCCGAACAC+TGG - chr1_3:57357289-57357308 None:intergenic 60.0%
CGACGATGGAGGAGGCAAGC+TGG + chr1_3:57356782-57356801 Msa0127760:CDS 65.0%
GAGGAGGCAAGCTGGAGCTC+TGG + chr1_3:57356790-57356809 Msa0127760:CDS 65.0%
CACAACGGCGACGCAACCGT+CGG + chr1_3:57356876-57356895 Msa0127760:CDS 65.0%
CTGTCTCAGCCGCCACTGCA+AGG - chr1_3:57356652-57356671 None:intergenic 65.0%
CGACGATGGAGGAGGCAAGC+TGG + chr1_3:57356782-57356801 Msa0127760:CDS 65.0%
GAGGAGGCAAGCTGGAGCTC+TGG + chr1_3:57356790-57356809 Msa0127760:CDS 65.0%
CACAACGGCGACGCAACCGT+CGG + chr1_3:57356876-57356895 Msa0127760:CDS 65.0%
CGACGGTTGCGTCGCCGTTG+TGG - chr1_3:57356878-57356897 None:intergenic 70.0%
CGACGGTTGCGTCGCCGTTG+TGG - chr1_3:57356878-57356897 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 57356651 57359010 57356651 ID=Msa0127760;Name=Msa0127760
chr1_3 mRNA 57356651 57359010 57356651 ID=Msa0127760-mRNA-1;Parent=Msa0127760;Name=Msa0127760-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|0.5|0.66|0.66|1|1|3|214|191
chr1_3 exon 57356651 57356899 57356651 ID=Msa0127760-mRNA-1:exon:18183;Parent=Msa0127760-mRNA-1
chr1_3 exon 57358199 57358460 57358199 ID=Msa0127760-mRNA-1:exon:18184;Parent=Msa0127760-mRNA-1
chr1_3 exon 57358732 57359010 57358732 ID=Msa0127760-mRNA-1:exon:18185;Parent=Msa0127760-mRNA-1
chr1_3 CDS 57356651 57356899 57356651 ID=Msa0127760-mRNA-1:cds;Parent=Msa0127760-mRNA-1
chr1_3 CDS 57358199 57358460 57358199 ID=Msa0127760-mRNA-1:cds;Parent=Msa0127760-mRNA-1
chr1_3 CDS 57358732 57358796 57358732 ID=Msa0127760-mRNA-1:cds;Parent=Msa0127760-mRNA-1
chr1_3 three_prime_UTR 57358797 57359010 57358797 ID=Msa0127760-mRNA-1:three_prime_utr;Parent=Msa0127760-mRNA-1
Gene Sequence

>Msa0127760

TTGCAGTGGCGGCTGAGACAGAGAAAGCTTGCGTCCAAACTAAATCTGAAGACAGCCGAAGAGCAGACACGAACGGAAGTTGAGGCCGCCAGAGACTTACCAACAACAAACCTCAAAAGACGGCAGCAAAACGACGATGGAGGAGGCAAGCTGGAGCTCTGGGTCTTGAAGGTGGAGACTAGGAACGGAGGAGCAGAAACCGCCATCTTATTGAACAAGGACAGCCACAACGGCGACGCAACCGTCGGAATCATGGACACTTCAAATCCTGCTGCATTTGTCAATGCCCAAGTTCTTCCCAATTTCATTGGAAAAAAAGTAAGAACAGTGGTTCAGGTGAATCATTCTGATGGTGCGACAATCACGGGAAATTCAACTGATGAATCTCAGATAACTGTAAAAGGGTTATCATCCCAGGTTCCTGTCATGAATTATGCTGAGGTTATTGGCATTGCTGAAAGTAACAACTCTATCCATGCTGAAATATTGACTGACTTTGGTGCCACATTTGACGTCAACTCTTACAATCAGCTATGCCAACTAGCAAATGGTGAATGCAGAAGTCTGTTTCTTTAG
Protein sequence

>Msa0127760

LQWRLRQRKLASKLNLKTAEEQTRTEVEAARDLPTTNLKRRQQNDDGGGKLELWVLKVETRNGGAETAILLNKDSHNGDATVGIMDTSNPAAFVNAQVLPNFIGKKVRTVVQVNHSDGATITGNSTDESQITVKGLSSQVPVMNYAEVIGIAESNNSIHAEILTDFGATFDVNSYNQLCQLANGECRSLFL*