Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0130480 | XP_003591828.2 | 62.025 | 474 | 109 | 5 | 630 | 1034 | 3 | 474 | 0.0 | 571 |
| Msa0130480 | XP_003591828.2 | 76.346 | 520 | 27 | 3 | 27 | 506 | 6 | 469 | 0.0 | 780 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0130480 | sp|Q6NQK2|NAC8_ARATH | 45.019 | 522 | 156 | 13 | 28 | 506 | 5 | 438 | 1.50e-119 | 377 |
| Msa0130480 | sp|Q6NQK2|NAC8_ARATH | 46.341 | 451 | 180 | 14 | 634 | 1034 | 5 | 443 | 6.00e-110 | 352 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0036740 | Msa0130480 | 0.851522 | 8.402967e-61 | -8.615850e-47 |
| Msa0130480 | Msa1425480 | 0.883193 | 5.518194e-71 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0130480 | MtrunA17_Chr1g0198751 | 76.346 | 520 | 27 | 3 | 27 | 506 | 6 | 469 | 0.0 | 780 |
| Msa0130480 | MtrunA17_Chr1g0198751 | 62.025 | 474 | 109 | 5 | 630 | 1034 | 3 | 474 | 0.0 | 571 |
| Msa0130480 | MtrunA17_Chr5g0422431 | 55.186 | 511 | 117 | 7 | 27 | 506 | 4 | 433 | 3.50e-172 | 511 |
| Msa0130480 | MtrunA17_Chr5g0422431 | 57.991 | 438 | 138 | 9 | 633 | 1030 | 4 | 435 | 2.35e-155 | 467 |
| Msa0130480 | MtrunA17_Chr1g0198741 | 82.550 | 298 | 32 | 4 | 741 | 1034 | 8 | 289 | 2.87e-168 | 494 |
| Msa0130480 | MtrunA17_Chr1g0198741 | 61.442 | 319 | 70 | 6 | 183 | 499 | 8 | 275 | 1.94e-113 | 352 |
| Msa0130480 | MtrunA17_Chr7g0227071 | 53.271 | 214 | 98 | 1 | 635 | 846 | 5 | 218 | 1.44e-72 | 249 |
| Msa0130480 | MtrunA17_Chr7g0227071 | 45.802 | 262 | 92 | 2 | 29 | 288 | 5 | 218 | 4.76e-70 | 242 |
| Msa0130480 | MtrunA17_Chr2g0282871 | 54.762 | 210 | 92 | 2 | 659 | 865 | 25 | 234 | 8.37e-67 | 227 |
| Msa0130480 | MtrunA17_Chr2g0282871 | 55.779 | 199 | 87 | 1 | 123 | 320 | 49 | 247 | 2.68e-65 | 223 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0130480 | AT1G25580.1 | 45.019 | 522 | 156 | 13 | 28 | 506 | 5 | 438 | 1.52e-120 | 377 |
| Msa0130480 | AT1G25580.1 | 46.341 | 451 | 180 | 14 | 634 | 1034 | 5 | 443 | 6.10e-111 | 352 |
| Msa0130480 | AT4G29230.3 | 61.714 | 175 | 58 | 2 | 122 | 287 | 43 | 217 | 1.78e-68 | 236 |
| Msa0130480 | AT4G29230.3 | 56.250 | 192 | 73 | 3 | 663 | 845 | 28 | 217 | 3.27e-65 | 227 |
| Msa0130480 | AT3G01600.1 | 51.339 | 224 | 104 | 3 | 634 | 855 | 4 | 224 | 7.53e-68 | 232 |
| Msa0130480 | AT3G01600.1 | 46.886 | 273 | 92 | 6 | 28 | 298 | 4 | 225 | 8.92e-68 | 232 |
| Msa0130480 | AT4G29230.1 | 61.714 | 175 | 58 | 2 | 122 | 287 | 43 | 217 | 1.03e-67 | 236 |
| Msa0130480 | AT4G29230.1 | 56.250 | 192 | 73 | 3 | 663 | 845 | 28 | 217 | 1.22e-64 | 227 |
| Msa0130480 | AT4G29230.2 | 61.494 | 174 | 58 | 2 | 123 | 287 | 43 | 216 | 4.74e-67 | 234 |
| Msa0130480 | AT4G29230.2 | 55.729 | 192 | 73 | 3 | 663 | 845 | 28 | 216 | 8.58e-64 | 225 |
Find 237 sgRNAs with CRISPR-Local
Find 1213 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGCTGAGAAAACTAACT+GGG | 0.630819 | 1_3:-60784491 | Msa0130480:CDS |
| AAAAGTACAGTCCATCTTGA+AGG | 0.486761 | 1_3:+60780497 | None:intergenic |
| AAAATATGTGGTCAAGATTT+TGG | 0.149918 | 1_3:-60784622 | Msa0130480:CDS |
| AAAGAACCTGGTCGCATCTT+CGG | 0.397326 | 1_3:+60780469 | None:intergenic |
| AAAGGAGAAAGACATAGCAG+AGG | 0.700278 | 1_3:-60780672 | Msa0130480:CDS |
| AAAGTTCTACCTGAGGAATA+TGG | 0.448471 | 1_3:+60770109 | None:intergenic |
| AAATTCATCGATGAATGGAT+GGG | 0.565244 | 1_3:+60784867 | None:intergenic |
| AAATTGGGACTGATAAGAGG+AGG | 0.689769 | 1_3:+60770156 | None:intergenic |
| AAGAACAGTGAACGCGTTGG+AGG | 0.558755 | 1_3:+60773355 | None:intergenic |
| AAGACATAGCAGAGGCATGG+CGG | 0.703822 | 1_3:-60780664 | Msa0130480:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACTGTTGATACTAAAGTAAG+AGG | + | chr1_3:60768908-60768927 | None:intergenic | 30.0% | |
| TCAGAAGGAATTATCATAGT+GGG | + | chr1_3:60769537-60769556 | None:intergenic | 30.0% | |
| ATCAGAAGGAATTATCATAG+TGG | + | chr1_3:60769538-60769557 | None:intergenic | 30.0% | |
| CTTATCTTAGTGTCTACAAT+TGG | + | chr1_3:60769647-60769666 | None:intergenic | 30.0% | |
| TTGGTATAACTAATCAAAGG+GGG | + | chr1_3:60769679-60769698 | None:intergenic | 30.0% | |
| CTCAAATAAAAAGCCACAAT+TGG | + | chr1_3:60769698-60769717 | None:intergenic | 30.0% | |
| AAGCAACCGAAAAAGTTATA+TGG | - | chr1_3:60769918-60769937 | Msa0130480:intron | 30.0% | |
| AGCAACCGAAAAAGTTATAT+GGG | - | chr1_3:60769919-60769938 | Msa0130480:intron | 30.0% | |
| CGATAACATGTTGACAATTT+GGG | + | chr1_3:60769967-60769986 | None:intergenic | 30.0% | |
| TCGATAACATGTTGACAATT+TGG | + | chr1_3:60769968-60769987 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_3 | gene | 60768826 | 60788187 | 60768826 | ID=Msa0130480;Name=Msa0130480 |
| chr1_3 | mRNA | 60768826 | 60788187 | 60768826 | ID=Msa0130480-mRNA-1;Parent=Msa0130480;Name=Msa0130480-mRNA-1;_AED=0.20;_eAED=0.20;_QI=27|0.12|0.29|0.58|1|1|17|0|1034 |
| chr1_3 | exon | 60768826 | 60768864 | 60768826 | ID=Msa0130480-mRNA-1:exon:20062;Parent=Msa0130480-mRNA-1 |
| chr1_3 | exon | 60768967 | 60769335 | 60768967 | ID=Msa0130480-mRNA-1:exon:20061;Parent=Msa0130480-mRNA-1 |
| chr1_3 | exon | 60770119 | 60770597 | 60770119 | ID=Msa0130480-mRNA-1:exon:20060;Parent=Msa0130480-mRNA-1 |
| chr1_3 | exon | 60770685 | 60770871 | 60770685 | ID=Msa0130480-mRNA-1:exon:20059;Parent=Msa0130480-mRNA-1 |
| chr1_3 | exon | 60773071 | 60773206 | 60773071 | ID=Msa0130480-mRNA-1:exon:20058;Parent=Msa0130480-mRNA-1 |
| chr1_3 | exon | 60773312 | 60773421 | 60773312 | ID=Msa0130480-mRNA-1:exon:20057;Parent=Msa0130480-mRNA-1 |
| chr1_3 | exon | 60780227 | 60780295 | 60780227 | ID=Msa0130480-mRNA-1:exon:20056;Parent=Msa0130480-mRNA-1 |
| chr1_3 | exon | 60780392 | 60780524 | 60780392 | ID=Msa0130480-mRNA-1:exon:20055;Parent=Msa0130480-mRNA-1 |
| chr1_3 | exon | 60780644 | 60780726 | 60780644 | ID=Msa0130480-mRNA-1:exon:20054;Parent=Msa0130480-mRNA-1 |
| chr1_3 | exon | 60783147 | 60783617 | 60783147 | ID=Msa0130480-mRNA-1:exon:20053;Parent=Msa0130480-mRNA-1 |
| chr1_3 | exon | 60784240 | 60784724 | 60784240 | ID=Msa0130480-mRNA-1:exon:20052;Parent=Msa0130480-mRNA-1 |
| chr1_3 | exon | 60784809 | 60784995 | 60784809 | ID=Msa0130480-mRNA-1:exon:20051;Parent=Msa0130480-mRNA-1 |
| chr1_3 | exon | 60786159 | 60786245 | 60786159 | ID=Msa0130480-mRNA-1:exon:20050;Parent=Msa0130480-mRNA-1 |
| chr1_3 | exon | 60786569 | 60786625 | 60786569 | ID=Msa0130480-mRNA-1:exon:20049;Parent=Msa0130480-mRNA-1 |
| chr1_3 | exon | 60787016 | 60787151 | 60787016 | ID=Msa0130480-mRNA-1:exon:20048;Parent=Msa0130480-mRNA-1 |
| chr1_3 | exon | 60787526 | 60787588 | 60787526 | ID=Msa0130480-mRNA-1:exon:20047;Parent=Msa0130480-mRNA-1 |
| chr1_3 | exon | 60788147 | 60788187 | 60788147 | ID=Msa0130480-mRNA-1:exon:20046;Parent=Msa0130480-mRNA-1 |
| chr1_3 | five_prime_UTR | 60788161 | 60788187 | 60788161 | ID=Msa0130480-mRNA-1:five_prime_utr;Parent=Msa0130480-mRNA-1 |
| chr1_3 | CDS | 60788147 | 60788160 | 60788147 | ID=Msa0130480-mRNA-1:cds;Parent=Msa0130480-mRNA-1 |
| chr1_3 | CDS | 60787526 | 60787588 | 60787526 | ID=Msa0130480-mRNA-1:cds;Parent=Msa0130480-mRNA-1 |
| chr1_3 | CDS | 60787016 | 60787151 | 60787016 | ID=Msa0130480-mRNA-1:cds;Parent=Msa0130480-mRNA-1 |
| chr1_3 | CDS | 60786569 | 60786625 | 60786569 | ID=Msa0130480-mRNA-1:cds;Parent=Msa0130480-mRNA-1 |
| chr1_3 | CDS | 60786159 | 60786245 | 60786159 | ID=Msa0130480-mRNA-1:cds;Parent=Msa0130480-mRNA-1 |
| chr1_3 | CDS | 60784809 | 60784995 | 60784809 | ID=Msa0130480-mRNA-1:cds;Parent=Msa0130480-mRNA-1 |
| chr1_3 | CDS | 60784240 | 60784724 | 60784240 | ID=Msa0130480-mRNA-1:cds;Parent=Msa0130480-mRNA-1 |
| chr1_3 | CDS | 60783147 | 60783617 | 60783147 | ID=Msa0130480-mRNA-1:cds;Parent=Msa0130480-mRNA-1 |
| chr1_3 | CDS | 60780644 | 60780726 | 60780644 | ID=Msa0130480-mRNA-1:cds;Parent=Msa0130480-mRNA-1 |
| chr1_3 | CDS | 60780392 | 60780524 | 60780392 | ID=Msa0130480-mRNA-1:cds;Parent=Msa0130480-mRNA-1 |
| chr1_3 | CDS | 60780227 | 60780295 | 60780227 | ID=Msa0130480-mRNA-1:cds;Parent=Msa0130480-mRNA-1 |
| chr1_3 | CDS | 60773312 | 60773421 | 60773312 | ID=Msa0130480-mRNA-1:cds;Parent=Msa0130480-mRNA-1 |
| chr1_3 | CDS | 60773071 | 60773206 | 60773071 | ID=Msa0130480-mRNA-1:cds;Parent=Msa0130480-mRNA-1 |
| chr1_3 | CDS | 60770685 | 60770871 | 60770685 | ID=Msa0130480-mRNA-1:cds;Parent=Msa0130480-mRNA-1 |
| chr1_3 | CDS | 60770119 | 60770597 | 60770119 | ID=Msa0130480-mRNA-1:cds;Parent=Msa0130480-mRNA-1 |
| chr1_3 | CDS | 60768967 | 60769335 | 60768967 | ID=Msa0130480-mRNA-1:cds;Parent=Msa0130480-mRNA-1 |
| chr1_3 | CDS | 60768826 | 60768864 | 60768826 | ID=Msa0130480-mRNA-1:cds;Parent=Msa0130480-mRNA-1 |
| Gene Sequence |
>Msa0130480 ATGGCTACGTCTGGAAACATCGAAAACCTGAATGTCGCTGCCACAATCGCTTCACAACTTGGTGTTGATAATCTCATACCGTCGTGGCTGGTGGACAAAAGGAGAATTGCAACAAAAATAAAGTGTGCATCTGAAGCAACCGAAAAAGTTATATGGGAAAGCAATCCTACCAGAGCTTGCCCAAATTGTCAACATGTTATCGACAACAGTGATGACATTCTGTTAAGTCTCCCAAATGCAGCCTTGCTCGGATTCTTGATAGCTGTTTTGTTTATTCATCCCCATCATAAGTTGAATTTATTGAGATTTTCACAACGTGCCAGGAAAATTCCTGAATCTATTGAGATTTCTGCACCTGTAACACAAGAGTGGCCTGGATTACCTAGAGGTGTTAAATTTGATCCATCTGATCAAGAGATAATATGGCACTTGCTTGCAAAAGTTGGTGAAGGAAATTTAGAACCCCATCCATTCATCGATGAATTTATCACTACTCTTAAAATGGATGATGGTATTTGTTATACTCATCCTCAGCGTTTACCAGGTGTTTCGCGAGATGGAATTGCTTCACACTTTTTCCATAGAGCAATCAAAGCCTATAATACTGGAAGCCGAAAGCGTCGAAAAATATGTGGTCAAGATTTTGGCGACGTCCGCTGGCACAAGACCGGAAGGACTAAACCTATCCTCTTGAACGGGCTTCAGAAAGGCTGTAAAAAGATTATGGTACTTTATATGACTCCAGAGAGTGGAGGAAAAGCTGAGAAAACTAACTGGGTTATGCATCAATACCATCTTGGAACAGAAGAGGATGAAAAGGATGGAGAATATGTTATTTCTAAAGTATTTTACCAGCAGCAGCAAGCCAAATTGGGTGAAAAAGATGACCAAAATGTTCCTGAAACCTCGGAAGCAACTGTTATAAAAGTGGATCCAGTCACTCCCAATTCCGTGACGCCTGAACCTCCTCGCAATGAGAAGCAATGTTCAGATGTAGACCAAGAACAAGAATCTCATCATAGTCCTCAGATTCTACCAGATCTTTCTCGGTTAGAATGGCTACTTGAAAGTCAGGATGACTGTGAAGAGCTTCCAAAAATTGACTCGTCGACTATAGAAACTCAAACTAATGAAGGGGTCGATAACATTGACCTGTCGCAGAATTTATTGAATTCACAGCAACTTGCGGAAGGACTTTCCTTGTTCGCAGATTTCTTTCAGAGCCAGTCTCCGAAAAGGGATGGGGAAAACGGAGAGCAAATGGAACGACCTAGTCTTTCTGTATATGCTCATTTAGGAGCAGAGCAATTGAAGAAAGATATTGAAGAGTGTCAAAATCTTGAACTTAACACTACAAAGAAGAATGATATTGAAGAATGCCTTAATCTTGACGCTGACACTGAAAAAAAGAGCGATATCGAAGAGTGCCAAAATCTCAACCTTGACACTGCAAATGTAGAGCTTGACTCACAACCTTCCGACATTCGATTAAGTCAACTGAGTCTCCTTCAAGATGATCAGAATGGCAACGGAAAGGAGAAAGACATAGCAGAGGCATGGCGGAGCCCGACAAAAAAGCTCAAAGTCTCCTTCAAGATGGACTGTACTTTTTACAGCCGAAGATGCGACCAGGTTCTTTTCTTTCGAACCAAGGATCATCATCTATACACTTCTTATAGTTACTTGGCGACTGAAGTTGTCCTTGGAGCAAAAAAGGACATGACAAGTGATAATCCTAATTTCTTTTCGAATGTTAATGTCAGTCATAATCCTCTGCTGCACGTCCCGCTTAATTGGGGGATTTGGTTTACCTTCATTGCTCATTGCAACCTCCAACGCGTTCACTGTTCTTGGGCTCCCACATCATCCATGCTTCAGGCCCATTGGGCCCAAGTACCATCGTGGTTGGTGGACCAAAGTACACTTGCAACCGCTATAAGGTGTGCATCTGGAATGACCGCAAAAGTTACATCAGAAAGTAATCCTACCAGAGCGTGCCCTAATTGTCAACATGTTGTGGACAATACTCATGTAACACAAGAGTGGCCTGGACTTCCTAAAGGTGTTAAATTTGATCCATCTGATCAAGAGATAATATGGCACTTGCTTGCAAAAGTTGGTGAAGGAAGTTCAAAACCTCACCCTTTCATCGATGCATTTATCACTACTCTTAAAATGGATGATGGCATTTGTTATACTCATCCTCAGCATTTGCCAGGTGTTTCACAAGATGGAATTGTTTCACACTTTTTCCATAGAGCAGTGAAAGCTTATAGTACTGGAAGTCGAAAGCGGCGAAGAATATGTGGTCAAGAGTTTGCTTATGTCCGCTGGCACAAGACTGGAAAGACTAAACCTATCTTCTTGAACGGAGTTCAGAAAGGCTGCAAAAAGATTATGGTTCTTTATATGTTTCCAGAGAATGGAGGAAAAGGTGCAAAAACTAACTGGGTTATGCATCAGTATCATCTTGGAACAGAAGAGGATGAGAGGGAAGGGAACTATGTTATATCTAAAGTATTTTACCAGCAGCAGCAAGCCATATTGGGAAGAAAAGATGATCAAGATGTTCCTGAAACCGCGGAAGCAACTATTGCGAATGTTGATCTTGTCACTCCCAATTTTGCGACCACTGAACCTCCTCTTATCAGTCCCAATTTTGTAGACCAAGAACAAGAAACTCACCATATTCCTCAGATTCTACCAAATCTTCCTCAGTTAGAAGAATGGCTACTTAATAGTCAGGATGACTGTGAAGGGCTTCCGAAAATTGATCTACCGACGGTAGAAACTCAATTTCATGTAGGGATTAATAACATTGACATGCCACTGAATCTATTGGATTCACTTCCGGAAACATTTTCTTGGTTCGAATATTTATTTCAAAGCCAGTCTCCGAAAAGGCACAGGGAAAATGACGAACAAAAGAACCAGCCTAGTCTTTCGACATATGATCATCTAGGACCAGAGCAGTTAAAGAAAGACATGGAAGAGTGCCAAAATCTTGACCACACTGGCAATGTAGAGCTTGACTCAGAATCTTCCGACATTCGATTAAGTCAACTGGAATTCAGCTCACAGGAGCTATATCGACTAGGGTGGTAA |
| Protein sequence |
>Msa0130480 MATSGNIENLNVAATIASQLGVDNLIPSWLVDKRRIATKIKCASEATEKVIWESNPTRACPNCQHVIDNSDDILLSLPNAALLGFLIAVLFIHPHHKLNLLRFSQRARKIPESIEISAPVTQEWPGLPRGVKFDPSDQEIIWHLLAKVGEGNLEPHPFIDEFITTLKMDDGICYTHPQRLPGVSRDGIASHFFHRAIKAYNTGSRKRRKICGQDFGDVRWHKTGRTKPILLNGLQKGCKKIMVLYMTPESGGKAEKTNWVMHQYHLGTEEDEKDGEYVISKVFYQQQQAKLGEKDDQNVPETSEATVIKVDPVTPNSVTPEPPRNEKQCSDVDQEQESHHSPQILPDLSRLEWLLESQDDCEELPKIDSSTIETQTNEGVDNIDLSQNLLNSQQLAEGLSLFADFFQSQSPKRDGENGEQMERPSLSVYAHLGAEQLKKDIEECQNLELNTTKKNDIEECLNLDADTEKKSDIEECQNLNLDTANVELDSQPSDIRLSQLSLLQDDQNGNGKEKDIAEAWRSPTKKLKVSFKMDCTFYSRRCDQVLFFRTKDHHLYTSYSYLATEVVLGAKKDMTSDNPNFFSNVNVSHNPLLHVPLNWGIWFTFIAHCNLQRVHCSWAPTSSMLQAHWAQVPSWLVDQSTLATAIRCASGMTAKVTSESNPTRACPNCQHVVDNTHVTQEWPGLPKGVKFDPSDQEIIWHLLAKVGEGSSKPHPFIDAFITTLKMDDGICYTHPQHLPGVSQDGIVSHFFHRAVKAYSTGSRKRRRICGQEFAYVRWHKTGKTKPIFLNGVQKGCKKIMVLYMFPENGGKGAKTNWVMHQYHLGTEEDEREGNYVISKVFYQQQQAILGRKDDQDVPETAEATIANVDLVTPNFATTEPPLISPNFVDQEQETHHIPQILPNLPQLEEWLLNSQDDCEGLPKIDLPTVETQFHVGINNIDMPLNLLDSLPETFSWFEYLFQSQSPKRHRENDEQKNQPSLSTYDHLGPEQLKKDMEECQNLDHTGNVELDSESSDIRLSQLEFSSQELYRLGW* |
