AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0130900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0130900 MtrunA17_Chr1g0199461 98.571 70 1 0 1 70 256 325 3.08e-44 149
Msa0130900 MtrunA17_Chr1g0180941 80.303 66 13 0 1 66 231 296 8.46e-32 115
Msa0130900 MtrunA17_Chr3g0120091 60.870 69 27 0 1 69 128 196 2.36e-24 94.0
Msa0130900 MtrunA17_Chr6g0461741 57.971 69 29 0 1 69 128 196 5.75e-23 90.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0130900 AT5G03330.3 68.675 83 22 1 1 83 251 329 5.97e-31 114
Msa0130900 AT5G03330.4 68.675 83 22 1 1 83 251 329 5.97e-31 114
Msa0130900 AT5G04250.2 51.429 105 45 2 6 110 247 345 1.43e-30 113
Msa0130900 AT5G04250.1 51.429 105 45 2 6 110 247 345 1.43e-30 113
Msa0130900 AT5G03330.1 75.714 70 17 0 1 70 251 320 4.14e-30 112
Msa0130900 AT5G03330.2 75.714 70 17 0 1 70 251 320 4.14e-30 112
Msa0130900 AT3G02070.1 60.000 65 26 0 1 65 117 181 3.63e-22 88.6
Msa0130900 AT3G02070.2 60.000 65 26 0 1 65 117 181 3.63e-22 88.6
Msa0130900 AT3G22260.1 56.522 69 30 0 1 69 139 207 9.89e-21 85.1
Msa0130900 AT3G22260.4 56.522 69 30 0 1 69 139 207 1.07e-20 85.1
Msa0130900 AT3G22260.3 56.522 69 30 0 1 69 139 207 1.07e-20 85.1
Msa0130900 AT3G22260.6 56.522 69 30 0 1 69 139 207 1.07e-20 85.1
Msa0130900 AT3G22260.5 56.522 69 30 0 1 69 139 207 1.07e-20 85.1
Msa0130900 AT3G22260.2 56.522 69 30 0 1 69 139 207 1.07e-20 85.1

Find 47 sgRNAs with CRISPR-Local

Find 291 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGTAATATTTCTGAGCTTT+TGG 0.231658 1_3:-61390326 Msa0130900:CDS
ATAAATTTGTTACCTCCTTC+CGG 0.346210 1_3:+61390276 None:intergenic
ATGGTGGAACTTTGGCGTTT+AGG 0.370980 1_3:-61389755 Msa0130900:CDS
AGAATAGAAGGAGGCGAAGA+GGG 0.380255 1_3:+61389543 None:intergenic
TATATACATAAAAGAATAGA+AGG 0.383736 1_3:+61389531 None:intergenic
GGGCGATCATGTCACGTTGC+AGG 0.384197 1_3:-61391594 Msa0130900:CDS
TACAAAGCCGGTCATACTAA+TGG 0.384307 1_3:+61389650 None:intergenic
CTATGCTGGTTATGTTCCTA+TGG 0.389215 1_3:-61391762 Msa0130900:CDS
ATTTGTGATTACATCTTTCA+AGG 0.399824 1_3:-61390652 Msa0130900:CDS
TGATCAAATCTTACTTCTTG+TGG 0.413550 1_3:+61390590 None:intergenic
ACATAACCAGCATAGAGATC+TGG 0.421382 1_3:+61391770 None:intergenic
GTTGCAGGCTGCTGCAGATT+GGG 0.423487 1_3:-61391579 Msa0130900:intron
CGTTGCAGGCTGCTGCAGAT+TGG 0.424955 1_3:-61391580 Msa0130900:CDS
AAGAATAGAAGGAGGCGAAG+AGG 0.435692 1_3:+61389542 None:intergenic
ACAATAATATGCAGCTAACT+TGG 0.442304 1_3:+61389445 None:intergenic
GCATTACAACTCAATTTATC+CGG 0.470926 1_3:-61390295 Msa0130900:CDS
AATATTTAAAGAAGATGAGC+AGG 0.478327 1_3:-61391730 Msa0130900:intron
AATTTGTTAGGAGTGGTGAA+TGG 0.479280 1_3:-61391616 Msa0130900:intron
AAGAAGAGATGGTGGAACTT+TGG 0.480199 1_3:-61389763 Msa0130900:CDS
AATCTAACATATTCTTAAGC+TGG 0.480575 1_3:-61389388 Msa0130900:three_prime_UTR
AGCTCAAATGATTACAAAGC+CGG 0.482549 1_3:+61389638 None:intergenic
ATTGGTTCCATTAGTATGAC+CGG 0.501743 1_3:-61389657 Msa0130900:exon
ACTGCATAAATCACAGGTAA+AGG 0.509232 1_3:+61390345 None:intergenic
CATAACCAGCATAGAGATCT+GGG 0.512866 1_3:+61391771 None:intergenic
GGAGGAACCAACTCTAGCTA+TGG 0.513218 1_3:+61389564 None:intergenic
AAATAAATATATAACGCAGC+TGG 0.521490 1_3:+61389608 None:intergenic
ATTTGTTAGGAGTGGTGAAT+GGG 0.523506 1_3:-61391615 Msa0130900:intron
AATATTACTGCATAAATCAC+AGG 0.529008 1_3:+61390339 None:intergenic
GTTTAGGATCAGCGCACAAA+TGG 0.537718 1_3:-61389739 Msa0130900:CDS
TCCGACCCAGATCTCTATGC+TGG 0.548139 1_3:-61391776 Msa0130900:CDS
AAATATTCACTATAAACCAT+AGG 0.553433 1_3:+61391746 None:intergenic
TACAACTCAATTTATCCGGA+AGG 0.580859 1_3:-61390291 Msa0130900:CDS
TCTGGTCAGATTGTCTGCAA+TGG 0.587731 1_3:+61389697 None:intergenic
GAGGAACCAACTCTAGCTAT+GGG 0.590658 1_3:+61389565 None:intergenic
ATACAACTATACACTGTACA+AGG 0.590665 1_3:+61389477 None:intergenic
TTATTCGCAACATATTGTTG+AGG 0.598375 1_3:-61389421 Msa0130900:three_prime_UTR
CCAGTGCCCATAGCTAGAGT+TGG 0.600161 1_3:-61389571 Msa0130900:three_prime_UTR
AACTCAATTTATCCGGAAGG+AGG 0.611887 1_3:-61390288 Msa0130900:intron
TATGGGCACTGGCAGTGTCA+AGG 0.619181 1_3:+61389582 None:intergenic
CCAACTCTAGCTATGGGCAC+TGG 0.620376 1_3:+61389571 None:intergenic
CTTGTGGACTTCTGTATCTG+CGG 0.625166 1_3:+61390606 None:intergenic
TTTGTTAGGAGTGGTGAATG+GGG 0.625472 1_3:-61391614 Msa0130900:intron
GGTCATACTAATGGAACCAA+TGG 0.630026 1_3:+61389659 None:intergenic
ATAGAAGGAGGCGAAGAGGG+AGG 0.650159 1_3:+61389546 None:intergenic
ACCAGCATAGAGATCTGGGT+CGG 0.658906 1_3:+61391775 None:intergenic
ATACATAAAAGAATAGAAGG+AGG 0.670501 1_3:+61389534 None:intergenic
ACACTGTACAAGGAAAACAA+AGG 0.735590 1_3:+61389487 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATTAAAATTGATGTAT+AGG + chr1_3:61389750-61389769 None:intergenic 10.0%
!!! ATCAATTTTAATTAATCAAA+TGG - chr1_3:61389754-61389773 Msa0130900:CDS 10.0%
!! AAAAATTATTTGTCTTTAAA+TGG - chr1_3:61390258-61390277 Msa0130900:intron 10.0%
!! TAAATTCTTTAATTATGTAT+AGG - chr1_3:61390335-61390354 Msa0130900:CDS 10.0%
!! ATTAATTAAAATTGATGTAT+AGG + chr1_3:61389750-61389769 None:intergenic 10.0%
!!! ATCAATTTTAATTAATCAAA+TGG - chr1_3:61389754-61389773 Msa0130900:CDS 10.0%
!! AAAAATTATTTGTCTTTAAA+TGG - chr1_3:61390258-61390277 Msa0130900:intron 10.0%
!! TAAATTCTTTAATTATGTAT+AGG - chr1_3:61390335-61390354 Msa0130900:CDS 10.0%
!! AAACAGAAGAAAATTATAAT+AGG + chr1_3:61389649-61389668 None:intergenic 15.0%
!!! TTCTATCATAAACTAAATTT+TGG + chr1_3:61389849-61389868 None:intergenic 15.0%
!!! TTAATTGTTATTAGGAAAAT+TGG + chr1_3:61389987-61390006 None:intergenic 15.0%
!! ATTGGTATTATAATCTTTAT+TGG - chr1_3:61390232-61390251 Msa0130900:intron 15.0%
!! TTAATTATGTATAGGAATTA+AGG - chr1_3:61390343-61390362 Msa0130900:CDS 15.0%
!! TATATACATAAAAGAATAGA+AGG + chr1_3:61391642-61391661 None:intergenic 15.0%
!! AAACAGAAGAAAATTATAAT+AGG + chr1_3:61389649-61389668 None:intergenic 15.0%
!!! TTCTATCATAAACTAAATTT+TGG + chr1_3:61389849-61389868 None:intergenic 15.0%
!!! TTAATTGTTATTAGGAAAAT+TGG + chr1_3:61389987-61390006 None:intergenic 15.0%
!! ATTGGTATTATAATCTTTAT+TGG - chr1_3:61390232-61390251 Msa0130900:intron 15.0%
!! TTAATTATGTATAGGAATTA+AGG - chr1_3:61390343-61390362 Msa0130900:CDS 15.0%
!! TATATACATAAAAGAATAGA+AGG + chr1_3:61391642-61391661 None:intergenic 15.0%
!! AAATATTCACTATAAACCAT+AGG + chr1_3:61389427-61389446 None:intergenic 20.0%
!! TTAAACCATTTGAACTATTT+GGG + chr1_3:61390168-61390187 None:intergenic 20.0%
!! TTTAATCCTTGATATATCAT+AGG - chr1_3:61390201-61390220 Msa0130900:intron 20.0%
!! TTTAAACCTATGATATATCA+AGG + chr1_3:61390210-61390229 None:intergenic 20.0%
!! ATATCATAGGTTTAAATGAT+TGG - chr1_3:61390214-61390233 Msa0130900:intron 20.0%
!! CACTCAAATTAAAATTTGAT+AGG + chr1_3:61390734-61390753 None:intergenic 20.0%
!!! ATCAAATTTTAATTTGAGTG+TGG - chr1_3:61390734-61390753 Msa0130900:intron 20.0%
!!! TATTTTAAAGATATGCTATG+AGG + chr1_3:61391252-61391271 None:intergenic 20.0%
!!! TTTTTCTTCAAATAAGATGA+AGG + chr1_3:61391389-61391408 None:intergenic 20.0%
!! AAATATTCACTATAAACCAT+AGG + chr1_3:61389427-61389446 None:intergenic 20.0%
!! TTAAACCATTTGAACTATTT+GGG + chr1_3:61390168-61390187 None:intergenic 20.0%
!! TTTAATCCTTGATATATCAT+AGG - chr1_3:61390201-61390220 Msa0130900:intron 20.0%
!! TTTAAACCTATGATATATCA+AGG + chr1_3:61390210-61390229 None:intergenic 20.0%
!! ATATCATAGGTTTAAATGAT+TGG - chr1_3:61390214-61390233 Msa0130900:intron 20.0%
!! CACTCAAATTAAAATTTGAT+AGG + chr1_3:61390734-61390753 None:intergenic 20.0%
!!! ATCAAATTTTAATTTGAGTG+TGG - chr1_3:61390734-61390753 Msa0130900:intron 20.0%
!!! TATTTTAAAGATATGCTATG+AGG + chr1_3:61391252-61391271 None:intergenic 20.0%
!!! TTTTTCTTCAAATAAGATGA+AGG + chr1_3:61391389-61391408 None:intergenic 20.0%
! AATATTTAAAGAAGATGAGC+AGG - chr1_3:61389440-61389459 Msa0130900:three_prime_UTR 25.0%
! TTATGTTTCTTATGCCAATA+TGG - chr1_3:61389488-61389507 Msa0130900:three_prime_UTR 25.0%
!! GCTTTATGTATGAATTTGTT+AGG - chr1_3:61389542-61389561 Msa0130900:three_prime_UTR 25.0%
! GAAGAAAATTATAATAGGCA+AGG + chr1_3:61389644-61389663 None:intergenic 25.0%
! TTAAAATAAACTCTGACACT+TGG + chr1_3:61389719-61389738 None:intergenic 25.0%
! TAAAATTGATGTATAGGAGT+TGG + chr1_3:61389744-61389763 None:intergenic 25.0%
!!! AGTTTTTCAAGGTCTAAATA+TGG + chr1_3:61389781-61389800 None:intergenic 25.0%
! TTAGACCTTGAAAAACTATA+AGG - chr1_3:61389784-61389803 Msa0130900:CDS 25.0%
! TAGACCTTGAAAAACTATAA+GGG - chr1_3:61389785-61389804 Msa0130900:CDS 25.0%
! AGTTTATGATAGAACATCAT+TGG - chr1_3:61389855-61389874 Msa0130900:intron 25.0%
!!! ACTGCGTTTTAATTGTTATT+AGG + chr1_3:61389995-61390014 None:intergenic 25.0%
!! ATTTGGGACAATTAACTATT+TGG + chr1_3:61390152-61390171 None:intergenic 25.0%
! CTTAAACCATTTGAACTATT+TGG + chr1_3:61390169-61390188 None:intergenic 25.0%
! ATAAGTAAATATCATGTCAC+CGG + chr1_3:61390306-61390325 None:intergenic 25.0%
! ATTTACTTGAATGACTGATA+TGG - chr1_3:61390460-61390479 Msa0130900:intron 25.0%
! ATTTGTGATTACATCTTTCA+AGG - chr1_3:61390518-61390537 Msa0130900:intron 25.0%
! AGTACATGTAAATGTGAATA+TGG - chr1_3:61390643-61390662 Msa0130900:CDS 25.0%
! AATATTACTGCATAAATCAC+AGG + chr1_3:61390834-61390853 None:intergenic 25.0%
!!! AGTAATATTTCTGAGCTTTT+GGG - chr1_3:61390845-61390864 Msa0130900:intron 25.0%
! TTTGTATATAGGAGATTTCA+TGG + chr1_3:61391021-61391040 None:intergenic 25.0%
!!! TTGGCTGTATTTTTGTATAT+AGG + chr1_3:61391032-61391051 None:intergenic 25.0%
! ATATACAAAAATACAGCCAA+TGG - chr1_3:61391032-61391051 Msa0130900:intron 25.0%
!! TCATATGGTTATATTTTCAG+AGG - chr1_3:61391296-61391315 Msa0130900:intron 25.0%
! ATTTGAAGAAAAAGAAGAGA+TGG - chr1_3:61391396-61391415 Msa0130900:intron 25.0%
! AAATAAATATATAACGCAGC+TGG + chr1_3:61391565-61391584 None:intergenic 25.0%
! ATACATAAAAGAATAGAAGG+AGG + chr1_3:61391639-61391658 None:intergenic 25.0%
! AATCTAACATATTCTTAAGC+TGG - chr1_3:61391782-61391801 Msa0130900:CDS 25.0%
! AATATTTAAAGAAGATGAGC+AGG - chr1_3:61389440-61389459 Msa0130900:three_prime_UTR 25.0%
! TTATGTTTCTTATGCCAATA+TGG - chr1_3:61389488-61389507 Msa0130900:three_prime_UTR 25.0%
!! GCTTTATGTATGAATTTGTT+AGG - chr1_3:61389542-61389561 Msa0130900:three_prime_UTR 25.0%
! GAAGAAAATTATAATAGGCA+AGG + chr1_3:61389644-61389663 None:intergenic 25.0%
! TTAAAATAAACTCTGACACT+TGG + chr1_3:61389719-61389738 None:intergenic 25.0%
! TAAAATTGATGTATAGGAGT+TGG + chr1_3:61389744-61389763 None:intergenic 25.0%
!!! AGTTTTTCAAGGTCTAAATA+TGG + chr1_3:61389781-61389800 None:intergenic 25.0%
! TTAGACCTTGAAAAACTATA+AGG - chr1_3:61389784-61389803 Msa0130900:CDS 25.0%
! TAGACCTTGAAAAACTATAA+GGG - chr1_3:61389785-61389804 Msa0130900:CDS 25.0%
! AGTTTATGATAGAACATCAT+TGG - chr1_3:61389855-61389874 Msa0130900:intron 25.0%
!!! ACTGCGTTTTAATTGTTATT+AGG + chr1_3:61389995-61390014 None:intergenic 25.0%
!! ATTTGGGACAATTAACTATT+TGG + chr1_3:61390152-61390171 None:intergenic 25.0%
! CTTAAACCATTTGAACTATT+TGG + chr1_3:61390169-61390188 None:intergenic 25.0%
! ATAAGTAAATATCATGTCAC+CGG + chr1_3:61390306-61390325 None:intergenic 25.0%
! ATTTACTTGAATGACTGATA+TGG - chr1_3:61390460-61390479 Msa0130900:intron 25.0%
! ATTTGTGATTACATCTTTCA+AGG - chr1_3:61390518-61390537 Msa0130900:intron 25.0%
! AGTACATGTAAATGTGAATA+TGG - chr1_3:61390643-61390662 Msa0130900:CDS 25.0%
! AATATTACTGCATAAATCAC+AGG + chr1_3:61390834-61390853 None:intergenic 25.0%
!!! AGTAATATTTCTGAGCTTTT+GGG - chr1_3:61390845-61390864 Msa0130900:intron 25.0%
! TTTGTATATAGGAGATTTCA+TGG + chr1_3:61391021-61391040 None:intergenic 25.0%
!!! TTGGCTGTATTTTTGTATAT+AGG + chr1_3:61391032-61391051 None:intergenic 25.0%
! ATATACAAAAATACAGCCAA+TGG - chr1_3:61391032-61391051 Msa0130900:intron 25.0%
!! TCATATGGTTATATTTTCAG+AGG - chr1_3:61391296-61391315 Msa0130900:intron 25.0%
! ATTTGAAGAAAAAGAAGAGA+TGG - chr1_3:61391396-61391415 Msa0130900:intron 25.0%
! AAATAAATATATAACGCAGC+TGG + chr1_3:61391565-61391584 None:intergenic 25.0%
! ATACATAAAAGAATAGAAGG+AGG + chr1_3:61391639-61391658 None:intergenic 25.0%
GACTTTCAATAAAGCCATAT+TGG + chr1_3:61389505-61389524 None:intergenic 30.0%
AAAGCTATCAGCAAAACATA+AGG + chr1_3:61389527-61389546 None:intergenic 30.0%
!! ATGTATGAATTTGTTAGGAG+TGG - chr1_3:61389547-61389566 Msa0130900:three_prime_UTR 30.0%
AGACCTTGAAAAACTATAAG+GGG - chr1_3:61389786-61389805 Msa0130900:CDS 30.0%
! TTTCCCCTTATAGTTTTTCA+AGG + chr1_3:61389792-61389811 None:intergenic 30.0%
AACTATAAGGGGAAACTATT+AGG - chr1_3:61389797-61389816 Msa0130900:intron 30.0%
TGTAATTTGATCCATGTAGA+GGG - chr1_3:61389878-61389897 Msa0130900:intron 30.0%
ATTGTCCCAAATAGTTCAAA+TGG - chr1_3:61390160-61390179 Msa0130900:intron 30.0%
TAAATGGAACTGCTGTAAAA+AGG - chr1_3:61390274-61390293 Msa0130900:intron 30.0%
ATCAGTCATTCAAGTAAATG+AGG + chr1_3:61390459-61390478 None:intergenic 30.0%
TGATCAAATCTTACTTCTTG+TGG + chr1_3:61390583-61390602 None:intergenic 30.0%
!!! ACTTAGCATTTTTCTGAAAC+AGG + chr1_3:61390799-61390818 None:intergenic 30.0%
!! CAGTAATATTTCTGAGCTTT+TGG - chr1_3:61390844-61390863 Msa0130900:intron 30.0%
GCATTACAACTCAATTTATC+CGG - chr1_3:61390875-61390894 Msa0130900:intron 30.0%
!! ATAAATTTGTTACCTCCTTC+CGG + chr1_3:61390897-61390916 None:intergenic 30.0%
!! TTTCATGGAGCGTTTTAAAT+TGG + chr1_3:61391006-61391025 None:intergenic 30.0%
TCTTATTGCTAGATAACCAT+TGG + chr1_3:61391051-61391070 None:intergenic 30.0%
!! CATTGCAGTTTTATGTAACA+TGG - chr1_3:61391159-61391178 Msa0130900:intron 30.0%
!! TACTGTTAGTTTGCTTCATA+TGG - chr1_3:61391281-61391300 Msa0130900:intron 30.0%
!!! ATTTTTGGCTCTTAAACCAT+TGG - chr1_3:61391495-61391514 Msa0130900:intron 30.0%
ATACAACTATACACTGTACA+AGG + chr1_3:61391696-61391715 None:intergenic 30.0%
ACAATAATATGCAGCTAACT+TGG + chr1_3:61391728-61391747 None:intergenic 30.0%
TTATTCGCAACATATTGTTG+AGG - chr1_3:61391749-61391768 Msa0130900:CDS 30.0%
GACTTTCAATAAAGCCATAT+TGG + chr1_3:61389505-61389524 None:intergenic 30.0%
AAAGCTATCAGCAAAACATA+AGG + chr1_3:61389527-61389546 None:intergenic 30.0%
!! ATGTATGAATTTGTTAGGAG+TGG - chr1_3:61389547-61389566 Msa0130900:three_prime_UTR 30.0%
AGACCTTGAAAAACTATAAG+GGG - chr1_3:61389786-61389805 Msa0130900:CDS 30.0%
! TTTCCCCTTATAGTTTTTCA+AGG + chr1_3:61389792-61389811 None:intergenic 30.0%
AACTATAAGGGGAAACTATT+AGG - chr1_3:61389797-61389816 Msa0130900:intron 30.0%
TGTAATTTGATCCATGTAGA+GGG - chr1_3:61389878-61389897 Msa0130900:intron 30.0%
ATTGTCCCAAATAGTTCAAA+TGG - chr1_3:61390160-61390179 Msa0130900:intron 30.0%
TAAATGGAACTGCTGTAAAA+AGG - chr1_3:61390274-61390293 Msa0130900:intron 30.0%
ATCAGTCATTCAAGTAAATG+AGG + chr1_3:61390459-61390478 None:intergenic 30.0%
TGATCAAATCTTACTTCTTG+TGG + chr1_3:61390583-61390602 None:intergenic 30.0%
!!! ACTTAGCATTTTTCTGAAAC+AGG + chr1_3:61390799-61390818 None:intergenic 30.0%
!! CAGTAATATTTCTGAGCTTT+TGG - chr1_3:61390844-61390863 Msa0130900:intron 30.0%
GCATTACAACTCAATTTATC+CGG - chr1_3:61390875-61390894 Msa0130900:intron 30.0%
!! ATAAATTTGTTACCTCCTTC+CGG + chr1_3:61390897-61390916 None:intergenic 30.0%
!! TTTCATGGAGCGTTTTAAAT+TGG + chr1_3:61391006-61391025 None:intergenic 30.0%
TCTTATTGCTAGATAACCAT+TGG + chr1_3:61391051-61391070 None:intergenic 30.0%
!! CATTGCAGTTTTATGTAACA+TGG - chr1_3:61391159-61391178 Msa0130900:intron 30.0%
!! TACTGTTAGTTTGCTTCATA+TGG - chr1_3:61391281-61391300 Msa0130900:intron 30.0%
!!! ATTTTTGGCTCTTAAACCAT+TGG - chr1_3:61391495-61391514 Msa0130900:intron 30.0%
ATACAACTATACACTGTACA+AGG + chr1_3:61391696-61391715 None:intergenic 30.0%
ACAATAATATGCAGCTAACT+TGG + chr1_3:61391728-61391747 None:intergenic 30.0%
TTATTCGCAACATATTGTTG+AGG - chr1_3:61391749-61391768 Msa0130900:CDS 30.0%
!! AATTTGTTAGGAGTGGTGAA+TGG - chr1_3:61389554-61389573 Msa0130900:three_prime_UTR 35.0%
! ATTTGTTAGGAGTGGTGAAT+GGG - chr1_3:61389555-61389574 Msa0130900:three_prime_UTR 35.0%
TGTGTGTGTGATATGATCAA+CGG - chr1_3:61389690-61389709 Msa0130900:CDS 35.0%
GGAAACTATTAGGAAAGACT+TGG - chr1_3:61389807-61389826 Msa0130900:intron 35.0%
GTGTAATTTGATCCATGTAG+AGG - chr1_3:61389877-61389896 Msa0130900:intron 35.0%
ACGCAGTCTTCAAAAAGAAT+TGG - chr1_3:61390008-61390027 Msa0130900:intron 35.0%
AGGATAAACTGAGTATTCTG+TGG - chr1_3:61390363-61390382 Msa0130900:CDS 35.0%
GGATAAACTGAGTATTCTGT+GGG - chr1_3:61390364-61390383 Msa0130900:CDS 35.0%
GAGGAATAAACAAAACGCAA+TGG + chr1_3:61390440-61390459 None:intergenic 35.0%
TTTCTCATGTCCTTTCAGTA+TGG - chr1_3:61390486-61390505 Msa0130900:intron 35.0%
!!! TATTTTGACGCCATACTGAA+AGG + chr1_3:61390499-61390518 None:intergenic 35.0%
ACTGCATAAATCACAGGTAA+AGG + chr1_3:61390828-61390847 None:intergenic 35.0%
TACAACTCAATTTATCCGGA+AGG - chr1_3:61390879-61390898 Msa0130900:intron 35.0%
! TGTCTGGAGTGTTTGTATAA+AGG + chr1_3:61390920-61390939 None:intergenic 35.0%
AATTTCCATTATCGTGTGTC+TGG + chr1_3:61390936-61390955 None:intergenic 35.0%
TCCATGCTCTTGAATTCATT+TGG - chr1_3:61391122-61391141 Msa0130900:intron 35.0%
ACCAAATGAATTCAAGAGCA+TGG + chr1_3:61391126-61391145 None:intergenic 35.0%
TGAAGAAAAAGAAGAGATGG+TGG - chr1_3:61391399-61391418 Msa0130900:intron 35.0%
! AGACAATCTGACCAGATTTT+TGG - chr1_3:61391480-61391499 Msa0130900:intron 35.0%
TGGTTTAAGAGCCAAAAATC+TGG + chr1_3:61391494-61391513 None:intergenic 35.0%
! ATTGGTTCCATTAGTATGAC+CGG - chr1_3:61391513-61391532 Msa0130900:intron 35.0%
AGCTCAAATGATTACAAAGC+CGG + chr1_3:61391535-61391554 None:intergenic 35.0%
ACACTGTACAAGGAAAACAA+AGG + chr1_3:61391686-61391705 None:intergenic 35.0%
!! AATTTGTTAGGAGTGGTGAA+TGG - chr1_3:61389554-61389573 Msa0130900:three_prime_UTR 35.0%
! ATTTGTTAGGAGTGGTGAAT+GGG - chr1_3:61389555-61389574 Msa0130900:three_prime_UTR 35.0%
TGTGTGTGTGATATGATCAA+CGG - chr1_3:61389690-61389709 Msa0130900:CDS 35.0%
GGAAACTATTAGGAAAGACT+TGG - chr1_3:61389807-61389826 Msa0130900:intron 35.0%
GTGTAATTTGATCCATGTAG+AGG - chr1_3:61389877-61389896 Msa0130900:intron 35.0%
ACGCAGTCTTCAAAAAGAAT+TGG - chr1_3:61390008-61390027 Msa0130900:intron 35.0%
AGGATAAACTGAGTATTCTG+TGG - chr1_3:61390363-61390382 Msa0130900:CDS 35.0%
GGATAAACTGAGTATTCTGT+GGG - chr1_3:61390364-61390383 Msa0130900:CDS 35.0%
GAGGAATAAACAAAACGCAA+TGG + chr1_3:61390440-61390459 None:intergenic 35.0%
TTTCTCATGTCCTTTCAGTA+TGG - chr1_3:61390486-61390505 Msa0130900:intron 35.0%
!!! TATTTTGACGCCATACTGAA+AGG + chr1_3:61390499-61390518 None:intergenic 35.0%
ACTGCATAAATCACAGGTAA+AGG + chr1_3:61390828-61390847 None:intergenic 35.0%
TACAACTCAATTTATCCGGA+AGG - chr1_3:61390879-61390898 Msa0130900:intron 35.0%
! TGTCTGGAGTGTTTGTATAA+AGG + chr1_3:61390920-61390939 None:intergenic 35.0%
AATTTCCATTATCGTGTGTC+TGG + chr1_3:61390936-61390955 None:intergenic 35.0%
TCCATGCTCTTGAATTCATT+TGG - chr1_3:61391122-61391141 Msa0130900:intron 35.0%
ACCAAATGAATTCAAGAGCA+TGG + chr1_3:61391126-61391145 None:intergenic 35.0%
TGAAGAAAAAGAAGAGATGG+TGG - chr1_3:61391399-61391418 Msa0130900:intron 35.0%
! AGACAATCTGACCAGATTTT+TGG - chr1_3:61391480-61391499 Msa0130900:intron 35.0%
TGGTTTAAGAGCCAAAAATC+TGG + chr1_3:61391494-61391513 None:intergenic 35.0%
! ATTGGTTCCATTAGTATGAC+CGG - chr1_3:61391513-61391532 Msa0130900:intron 35.0%
AGCTCAAATGATTACAAAGC+CGG + chr1_3:61391535-61391554 None:intergenic 35.0%
ACACTGTACAAGGAAAACAA+AGG + chr1_3:61391686-61391705 None:intergenic 35.0%
CATAACCAGCATAGAGATCT+GGG + chr1_3:61389402-61389421 None:intergenic 40.0%
ACATAACCAGCATAGAGATC+TGG + chr1_3:61389403-61389422 None:intergenic 40.0%
! CTATGCTGGTTATGTTCCTA+TGG - chr1_3:61389408-61389427 Msa0130900:three_prime_UTR 40.0%
TTTGTTAGGAGTGGTGAATG+GGG - chr1_3:61389556-61389575 Msa0130900:three_prime_UTR 40.0%
AGACTTGGAGATCAATGAGT+TGG - chr1_3:61389822-61389841 Msa0130900:intron 40.0%
GAGTTTAGACCAACAAGAGA+GGG + chr1_3:61389965-61389984 None:intergenic 40.0%
TAAACTGAGTATTCTGTGGG+TGG - chr1_3:61390367-61390386 Msa0130900:CDS 40.0%
AAACTGAGTATTCTGTGGGT+GGG - chr1_3:61390368-61390387 Msa0130900:intron 40.0%
!!! GCATGTAGTGTGTTTTGAAG+TGG - chr1_3:61390404-61390423 Msa0130900:intron 40.0%
AACTCAATTTATCCGGAAGG+AGG - chr1_3:61390882-61390901 Msa0130900:intron 40.0%
AGAACCAATACTCTCTGAGT+TGG - chr1_3:61391068-61391087 Msa0130900:intron 40.0%
! AAAGCCAACTCAGAGAGTAT+TGG + chr1_3:61391075-61391094 None:intergenic 40.0%
!! AAGAAGAGATGGTGGAACTT+TGG - chr1_3:61391407-61391426 Msa0130900:intron 40.0%
GGTCATACTAATGGAACCAA+TGG + chr1_3:61391514-61391533 None:intergenic 40.0%
TACAAAGCCGGTCATACTAA+TGG + chr1_3:61391523-61391542 None:intergenic 40.0%
CATAACCAGCATAGAGATCT+GGG + chr1_3:61389402-61389421 None:intergenic 40.0%
ACATAACCAGCATAGAGATC+TGG + chr1_3:61389403-61389422 None:intergenic 40.0%
! CTATGCTGGTTATGTTCCTA+TGG - chr1_3:61389408-61389427 Msa0130900:three_prime_UTR 40.0%
TTTGTTAGGAGTGGTGAATG+GGG - chr1_3:61389556-61389575 Msa0130900:three_prime_UTR 40.0%
AGACTTGGAGATCAATGAGT+TGG - chr1_3:61389822-61389841 Msa0130900:intron 40.0%
GAGTTTAGACCAACAAGAGA+GGG + chr1_3:61389965-61389984 None:intergenic 40.0%
TAAACTGAGTATTCTGTGGG+TGG - chr1_3:61390367-61390386 Msa0130900:CDS 40.0%
AAACTGAGTATTCTGTGGGT+GGG - chr1_3:61390368-61390387 Msa0130900:intron 40.0%
!!! GCATGTAGTGTGTTTTGAAG+TGG - chr1_3:61390404-61390423 Msa0130900:intron 40.0%
AACTCAATTTATCCGGAAGG+AGG - chr1_3:61390882-61390901 Msa0130900:intron 40.0%
AGAACCAATACTCTCTGAGT+TGG - chr1_3:61391068-61391087 Msa0130900:intron 40.0%
! AAAGCCAACTCAGAGAGTAT+TGG + chr1_3:61391075-61391094 None:intergenic 40.0%
!! AAGAAGAGATGGTGGAACTT+TGG - chr1_3:61391407-61391426 Msa0130900:intron 40.0%
GGTCATACTAATGGAACCAA+TGG + chr1_3:61391514-61391533 None:intergenic 40.0%
TACAAAGCCGGTCATACTAA+TGG + chr1_3:61391523-61391542 None:intergenic 40.0%
TGCTGCAGATTGGGTATGAA+GGG - chr1_3:61389600-61389619 Msa0130900:three_prime_UTR 45.0%
! AAGCCTTTTCCCACTAAGTG+GGG + chr1_3:61389904-61389923 None:intergenic 45.0%
! CAAGCCTTTTCCCACTAAGT+GGG + chr1_3:61389905-61389924 None:intergenic 45.0%
! GGGCTCACAAAGATTTTGGA+AGG + chr1_3:61389945-61389964 None:intergenic 45.0%
! GAGAGGGCTCACAAAGATTT+TGG + chr1_3:61389949-61389968 None:intergenic 45.0%
GGAGTTTAGACCAACAAGAG+AGG + chr1_3:61389966-61389985 None:intergenic 45.0%
GGAACTGCTGTAAAAAGGTG+AGG - chr1_3:61390279-61390298 Msa0130900:intron 45.0%
TGCTGTAAAAAGGTGAGGAC+CGG - chr1_3:61390284-61390303 Msa0130900:intron 45.0%
CTTGTGGACTTCTGTATCTG+CGG + chr1_3:61390567-61390586 None:intergenic 45.0%
AGTGTGGTGCATAATCTCTG+TGG - chr1_3:61390750-61390769 Msa0130900:intron 45.0%
ATCTCTGTGGTGTGTACTGT+TGG - chr1_3:61390763-61390782 Msa0130900:intron 45.0%
! ATTTCTGAGCTTTTGGGCAG+AGG - chr1_3:61390851-61390870 Msa0130900:intron 45.0%
ACACTCCAGACACACGATAA+TGG - chr1_3:61390928-61390947 Msa0130900:intron 45.0%
! TTCAAGAGCATGGAAGAGAC+TGG + chr1_3:61391116-61391135 None:intergenic 45.0%
! ATGGTGGAACTTTGGCGTTT+AGG - chr1_3:61391415-61391434 Msa0130900:intron 45.0%
! GTTTAGGATCAGCGCACAAA+TGG - chr1_3:61391431-61391450 Msa0130900:intron 45.0%
TCTGGTCAGATTGTCTGCAA+TGG + chr1_3:61391476-61391495 None:intergenic 45.0%
GAGGAACCAACTCTAGCTAT+GGG + chr1_3:61391608-61391627 None:intergenic 45.0%
AGAATAGAAGGAGGCGAAGA+GGG + chr1_3:61391630-61391649 None:intergenic 45.0%
AAGAATAGAAGGAGGCGAAG+AGG + chr1_3:61391631-61391650 None:intergenic 45.0%
TGCTGCAGATTGGGTATGAA+GGG - chr1_3:61389600-61389619 Msa0130900:three_prime_UTR 45.0%
! AAGCCTTTTCCCACTAAGTG+GGG + chr1_3:61389904-61389923 None:intergenic 45.0%
! CAAGCCTTTTCCCACTAAGT+GGG + chr1_3:61389905-61389924 None:intergenic 45.0%
! GGGCTCACAAAGATTTTGGA+AGG + chr1_3:61389945-61389964 None:intergenic 45.0%
! GAGAGGGCTCACAAAGATTT+TGG + chr1_3:61389949-61389968 None:intergenic 45.0%
GGAGTTTAGACCAACAAGAG+AGG + chr1_3:61389966-61389985 None:intergenic 45.0%
GGAACTGCTGTAAAAAGGTG+AGG - chr1_3:61390279-61390298 Msa0130900:intron 45.0%
TGCTGTAAAAAGGTGAGGAC+CGG - chr1_3:61390284-61390303 Msa0130900:intron 45.0%
CTTGTGGACTTCTGTATCTG+CGG + chr1_3:61390567-61390586 None:intergenic 45.0%
AGTGTGGTGCATAATCTCTG+TGG - chr1_3:61390750-61390769 Msa0130900:intron 45.0%
ATCTCTGTGGTGTGTACTGT+TGG - chr1_3:61390763-61390782 Msa0130900:intron 45.0%
! ATTTCTGAGCTTTTGGGCAG+AGG - chr1_3:61390851-61390870 Msa0130900:intron 45.0%
ACACTCCAGACACACGATAA+TGG - chr1_3:61390928-61390947 Msa0130900:intron 45.0%
! TTCAAGAGCATGGAAGAGAC+TGG + chr1_3:61391116-61391135 None:intergenic 45.0%
! ATGGTGGAACTTTGGCGTTT+AGG - chr1_3:61391415-61391434 Msa0130900:intron 45.0%
! GTTTAGGATCAGCGCACAAA+TGG - chr1_3:61391431-61391450 Msa0130900:intron 45.0%
TCTGGTCAGATTGTCTGCAA+TGG + chr1_3:61391476-61391495 None:intergenic 45.0%
GAGGAACCAACTCTAGCTAT+GGG + chr1_3:61391608-61391627 None:intergenic 45.0%
AGAATAGAAGGAGGCGAAGA+GGG + chr1_3:61391630-61391649 None:intergenic 45.0%
AAGAATAGAAGGAGGCGAAG+AGG + chr1_3:61391631-61391650 None:intergenic 45.0%
ACCAGCATAGAGATCTGGGT+CGG + chr1_3:61389398-61389417 None:intergenic 50.0%
CTGCTGCAGATTGGGTATGA+AGG - chr1_3:61389599-61389618 Msa0130900:three_prime_UTR 50.0%
CACACACACACATGCATGCA+TGG + chr1_3:61389678-61389697 None:intergenic 50.0%
! CCAAGCCTTTTCCCACTAAG+TGG + chr1_3:61389906-61389925 None:intergenic 50.0%
CCACTTAGTGGGAAAAGGCT+TGG - chr1_3:61389903-61389922 Msa0130900:intron 50.0%
CTTTGTGAGCCCTCTCTTGT+TGG - chr1_3:61389953-61389972 Msa0130900:intron 50.0%
GGAGGAACCAACTCTAGCTA+TGG + chr1_3:61391609-61391628 None:intergenic 50.0%
ACCAGCATAGAGATCTGGGT+CGG + chr1_3:61389398-61389417 None:intergenic 50.0%
CTGCTGCAGATTGGGTATGA+AGG - chr1_3:61389599-61389618 Msa0130900:three_prime_UTR 50.0%
CACACACACACATGCATGCA+TGG + chr1_3:61389678-61389697 None:intergenic 50.0%
! CCAAGCCTTTTCCCACTAAG+TGG + chr1_3:61389906-61389925 None:intergenic 50.0%
CCACTTAGTGGGAAAAGGCT+TGG - chr1_3:61389903-61389922 Msa0130900:intron 50.0%
CTTTGTGAGCCCTCTCTTGT+TGG - chr1_3:61389953-61389972 Msa0130900:intron 50.0%
GGAGGAACCAACTCTAGCTA+TGG + chr1_3:61391609-61391628 None:intergenic 50.0%
TCCGACCCAGATCTCTATGC+TGG - chr1_3:61389394-61389413 Msa0130900:three_prime_UTR 55.0%
GTTGCAGGCTGCTGCAGATT+GGG - chr1_3:61389591-61389610 Msa0130900:three_prime_UTR 55.0%
ACTAAGTGGGGCCCTCTACA+TGG + chr1_3:61389892-61389911 None:intergenic 55.0%
ATGTAGAGGGCCCCACTTAG+TGG - chr1_3:61389891-61389910 Msa0130900:intron 55.0%
TGTAGAGGGCCCCACTTAGT+GGG - chr1_3:61389892-61389911 Msa0130900:intron 55.0%
!! TATGGGCACTGGCAGTGTCA+AGG + chr1_3:61391591-61391610 None:intergenic 55.0%
CCAACTCTAGCTATGGGCAC+TGG + chr1_3:61391602-61391621 None:intergenic 55.0%
CCAGTGCCCATAGCTAGAGT+TGG - chr1_3:61391599-61391618 Msa0130900:CDS 55.0%
ATAGAAGGAGGCGAAGAGGG+AGG + chr1_3:61391627-61391646 None:intergenic 55.0%
TCCGACCCAGATCTCTATGC+TGG - chr1_3:61389394-61389413 Msa0130900:three_prime_UTR 55.0%
GTTGCAGGCTGCTGCAGATT+GGG - chr1_3:61389591-61389610 Msa0130900:three_prime_UTR 55.0%
ACTAAGTGGGGCCCTCTACA+TGG + chr1_3:61389892-61389911 None:intergenic 55.0%
ATGTAGAGGGCCCCACTTAG+TGG - chr1_3:61389891-61389910 Msa0130900:intron 55.0%
TGTAGAGGGCCCCACTTAGT+GGG - chr1_3:61389892-61389911 Msa0130900:intron 55.0%
!! TATGGGCACTGGCAGTGTCA+AGG + chr1_3:61391591-61391610 None:intergenic 55.0%
CCAACTCTAGCTATGGGCAC+TGG + chr1_3:61391602-61391621 None:intergenic 55.0%
CCAGTGCCCATAGCTAGAGT+TGG - chr1_3:61391599-61391618 Msa0130900:CDS 55.0%
ATAGAAGGAGGCGAAGAGGG+AGG + chr1_3:61391627-61391646 None:intergenic 55.0%
GGGCGATCATGTCACGTTGC+AGG - chr1_3:61389576-61389595 Msa0130900:three_prime_UTR 60.0%
CGTTGCAGGCTGCTGCAGAT+TGG - chr1_3:61389590-61389609 Msa0130900:three_prime_UTR 60.0%
GGGCCCCACTTAGTGGGAAA+AGG - chr1_3:61389898-61389917 Msa0130900:intron 60.0%
GGGCGATCATGTCACGTTGC+AGG - chr1_3:61389576-61389595 Msa0130900:three_prime_UTR 60.0%
CGTTGCAGGCTGCTGCAGAT+TGG - chr1_3:61389590-61389609 Msa0130900:three_prime_UTR 60.0%
GGGCCCCACTTAGTGGGAAA+AGG - chr1_3:61389898-61389917 Msa0130900:intron 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 61389388 61391804 61389388 ID=Msa0130900;Name=Msa0130900
chr1_3 mRNA 61389388 61391804 61389388 ID=Msa0130900-mRNA-1;Parent=Msa0130900;Name=Msa0130900-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0.6|0.66|0.83|0.6|0.66|6|273|153
chr1_3 exon 61389388 61389811 61389388 ID=Msa0130900-mRNA-1:exon:20435;Parent=Msa0130900-mRNA-1
chr1_3 exon 61390289 61390386 61390289 ID=Msa0130900-mRNA-1:exon:20434;Parent=Msa0130900-mRNA-1
chr1_3 exon 61390543 61390547 61390543 ID=Msa0130900-mRNA-1:exon:20433;Parent=Msa0130900-mRNA-1
chr1_3 exon 61390604 61390688 61390604 ID=Msa0130900-mRNA-1:exon:20432;Parent=Msa0130900-mRNA-1
chr1_3 exon 61391580 61391628 61391580 ID=Msa0130900-mRNA-1:exon:20431;Parent=Msa0130900-mRNA-1
chr1_3 exon 61391731 61391804 61391731 ID=Msa0130900-mRNA-1:exon:20430;Parent=Msa0130900-mRNA-1
chr1_3 CDS 61391731 61391804 61391731 ID=Msa0130900-mRNA-1:cds;Parent=Msa0130900-mRNA-1
chr1_3 CDS 61391580 61391628 61391580 ID=Msa0130900-mRNA-1:cds;Parent=Msa0130900-mRNA-1
chr1_3 CDS 61390604 61390688 61390604 ID=Msa0130900-mRNA-1:cds;Parent=Msa0130900-mRNA-1
chr1_3 CDS 61390543 61390547 61390543 ID=Msa0130900-mRNA-1:cds;Parent=Msa0130900-mRNA-1
chr1_3 CDS 61390289 61390386 61390289 ID=Msa0130900-mRNA-1:cds;Parent=Msa0130900-mRNA-1
chr1_3 CDS 61389661 61389811 61389661 ID=Msa0130900-mRNA-1:cds;Parent=Msa0130900-mRNA-1
chr1_3 three_prime_UTR 61389388 61389660 61389388 ID=Msa0130900-mRNA-1:three_prime_utr;Parent=Msa0130900-mRNA-1
Gene Sequence

>Msa0130900

CTCAAGTCCGACCCAGATCTCTATGCTGGTTATGTTCCTATGGTTTATAGTGAATATTTAAAGAAGATGAGCAGGAGTGGTGAATGGGGCGATCATGTCACGTTGCAGGCTGCTGCAGATTGGTATGGCGTCAAAATATTTGTGATTACATCTTTCAAGGACACATGTTACATTGAGATTCTTCCGCAGATACAGAAGTCCACAAGAATACATAAAAATGCTAAGTGTATGACCTTTACCTGTGATTTATGCAGTAATATTTCTGAGCTTTTGGGCAGAGGTGCATTACAACTCAATTTATCCGGAAGGAGAAATGCCTTCATCTTATTTGAAGAAAAAGAAGAGATGGTGGAACTTTGGCGTTTAGGATCAGCGCACAAATGGATGCAACTATTTCATGCATCCATTGCAGACAATCTGACCAGATTTTTGGCTCTTAAACCATTGGTTCCATTAGTATGA
Protein sequence

>Msa0130900

LKSDPDLYAGYVPMVYSEYLKKMSRSGEWGDHVTLQAAADWYGVKIFVITSFKDTCYIEILPQIQKSTRIHKNAKCMTFTCDLCSNISELLGRGALQLNLSGRRNAFILFEEKEEMVELWRLGSAHKWMQLFHASIADNLTRFLALKPLVPLV*