AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0131240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0131240 MtrunA17_Chr1g0199931 98.742 318 3 1 1 318 1 317 0.0 635
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0131240 AT3G07950.1 66.890 299 99 0 20 318 6 304 1.67e-144 409

Find 99 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAATTAAAATATTCCTTTA+AGG 0.111127 1_3:-61996701 None:intergenic
TCTAGCTATACGCCACTTTC+TGG 0.171913 1_3:+61995501 Msa0131240:intron
ATAGTTTGTCTTCTCTTAAT+TGG 0.213685 1_3:+61994997 Msa0131240:CDS
TATCTCCCAACTGTTATATT+TGG 0.226590 1_3:+61995795 Msa0131240:CDS
GTTCTATTGTTCTGAGCTTC+TGG 0.234816 1_3:+61995754 Msa0131240:CDS
GCAAGTTGCTTGAGCCAGTA+TGG 0.253816 1_3:+61995019 Msa0131240:CDS
GGATTTCATGGAGTGATATC+TGG 0.288533 1_3:+61995522 Msa0131240:CDS
ATTGCTTATCGATCCAATAT+TGG 0.294681 1_3:+61996849 Msa0131240:three_prime_UTR
TGATTCTTGACATCCAATAT+TGG 0.294853 1_3:-61996862 None:intergenic
TGGAGTGATATCTGGCTTCT+TGG 0.301472 1_3:+61995530 Msa0131240:CDS
TTATCGACTGTGTCGGTGTC+TGG 0.303188 1_3:+61994156 Msa0131240:CDS
ACGCCACTTTCTGGATTTCA+TGG 0.317090 1_3:+61995510 Msa0131240:CDS
TATCTGAATTTACAAAATCT+AGG 0.321788 1_3:-61996604 None:intergenic
CGCTCATGATAGTTTCATAT+TGG 0.322870 1_3:-61996634 None:intergenic
TCACCCTTAAGCTTTGTTTC+CGG 0.323823 1_3:-61995861 None:intergenic
AGACAAATAAAAGAAAGAAT+TGG 0.330289 1_3:+61993967 Msa0131240:five_prime_UTR
TTTGTAAATTCAGATAAGTT+TGG 0.343360 1_3:+61996611 Msa0131240:three_prime_UTR
CGATGAAATATTGATGCAAT+AGG 0.351484 1_3:-61996042 None:intergenic
ATAACTTACCTTAAAATCTC+TGG 0.362264 1_3:-61995918 None:intergenic
TTGGCTACTTCGCGGAGTGC+AGG 0.365183 1_3:+61996544 Msa0131240:CDS
AACAGTTTGTTCAACGTAAC+CGG 0.368690 1_3:-61994850 None:intergenic
TACGTTGAACAAACTGTTCA+TGG 0.372400 1_3:+61994855 Msa0131240:CDS
CGTCTCCTAGATGCCTCAAT+GGG 0.379047 1_3:-61996144 None:intergenic
GATGCTGAAGATTACGATCT+TGG 0.405414 1_3:+61996096 Msa0131240:CDS
GACAAATAAAAGAAAGAATT+GGG 0.407285 1_3:+61993968 Msa0131240:five_prime_UTR
AATTCCCTGGGACCCCATAC+TGG 0.413994 1_3:-61995033 None:intergenic
GATGCAATAGGATCTATTAC+TGG 0.414267 1_3:-61996030 None:intergenic
AATCAAGATAAACTAGTTGT+TGG 0.419481 1_3:+61996880 Msa0131240:three_prime_UTR
CCAACCTTTCTTCCAGTGCT+CGG 0.426478 1_3:-61996510 None:intergenic
CAAGTTGCTTGAGCCAGTAT+GGG 0.433068 1_3:+61995020 Msa0131240:CDS
GATGAACTTTATAAATTCCC+TGG 0.433979 1_3:-61995046 None:intergenic
AGAAAGAGGTGCCCGAGCAC+TGG 0.436362 1_3:+61996498 Msa0131240:CDS
CTTGGAAGTGGACATTTGCC+AGG 0.442134 1_3:+61996114 Msa0131240:CDS
GAACACAATAAAGCGATAGA+TGG 0.444977 1_3:-61995735 None:intergenic
GCTTCTTCTGCCAGTATCTC+AGG 0.451123 1_3:-61995839 None:intergenic
CTGACCCCATTGAGGCATCT+AGG 0.457185 1_3:+61996139 Msa0131240:CDS
GCTGGATTTACCTGAGATAC+TGG 0.457712 1_3:+61995829 Msa0131240:CDS
TGGCTACTTCGCGGAGTGCA+GGG 0.459320 1_3:+61996545 Msa0131240:CDS
ACCGGCTGTAATGAGGTTCC+AGG 0.465128 1_3:-61994832 None:intergenic
GAAGCCGGAAACAAAGCTTA+AGG 0.465257 1_3:+61995857 Msa0131240:CDS
CCGTCTCCTAGATGCCTCAA+TGG 0.465259 1_3:-61996145 None:intergenic
GCAGGACAATACCATTTGCC+TGG 0.468808 1_3:+61994814 Msa0131240:intron
GCCTCAATGGGGTCAGAACC+TGG 0.474004 1_3:-61996132 None:intergenic
CAACCTTTCTTCCAGTGCTC+GGG 0.477825 1_3:-61996509 None:intergenic
GCGAGGTAGGTGAGAGCGGA+AGG 0.478280 1_3:-61994246 None:intergenic
TATGTACCAAATATAACAGT+TGG 0.478340 1_3:-61995801 None:intergenic
TTGAGCCAGTATGGGGTCCC+AGG 0.478497 1_3:+61995028 Msa0131240:CDS
ATCTGAATTTACAAAATCTA+GGG 0.480923 1_3:-61996603 None:intergenic
TATTTGGTACATATATGAGC+TGG 0.486230 1_3:+61995811 Msa0131240:CDS
TCTGAGCTTCTGGATAATAG+AGG 0.494967 1_3:+61995764 Msa0131240:CDS
AAGCCGGAAACAAAGCTTAA+GGG 0.498827 1_3:+61995858 Msa0131240:CDS
ACGTTGAACAAACTGTTCAT+GGG 0.500320 1_3:+61994856 Msa0131240:CDS
TTTGTACTACATTACAAGAC+AGG 0.505647 1_3:+61995119 Msa0131240:CDS
TAACTTACCTTAAAATCTCT+GGG 0.509944 1_3:-61995917 None:intergenic
GTAATGAGGTTCCAGGCAAA+TGG 0.511611 1_3:-61994825 None:intergenic
GAAGATTACGATCTTGGAAG+TGG 0.512476 1_3:+61996102 Msa0131240:CDS
ACGAAAGGAAGCTCTTGATC+TGG 0.516753 1_3:-61995573 None:intergenic
GTGCCCGAGCACTGGAAGAA+AGG 0.518496 1_3:+61996506 Msa0131240:CDS
GCCTGGAACCTCATTACAGC+CGG 0.524904 1_3:+61994831 Msa0131240:CDS
ACCTCGCTCTCATTCCCGCC+AGG 0.526374 1_3:+61994262 Msa0131240:CDS
TCCCGTCTCATCACAGTCAC+AGG 0.530989 1_3:+61994040 Msa0131240:CDS
GAGAATGCAAAATCCTCACT+TGG 0.534096 1_3:-61995885 None:intergenic
TGATTATACAACTGCATGGA+AGG 0.535217 1_3:+61996813 Msa0131240:three_prime_UTR
CCGAGCACTGGAAGAAAGGT+TGG 0.540323 1_3:+61996510 Msa0131240:CDS
ATGAACTTTATAAATTCCCT+GGG 0.543126 1_3:-61995045 None:intergenic
GAGATACTGGCAGAAGAAGC+CGG 0.547275 1_3:+61995842 Msa0131240:CDS
GACTGTGATCTTCATGTGAG+TGG 0.547508 1_3:+61996734 Msa0131240:three_prime_UTR
ACTCCATGAAATCCAGAAAG+TGG 0.547828 1_3:-61995513 None:intergenic
GAGAGCGAGGTAGGTGAGAG+CGG 0.549328 1_3:-61994250 None:intergenic
GCCAGGTTCTGACCCCATTG+AGG 0.551934 1_3:+61996131 Msa0131240:CDS
TGGATTCACACGACTCTGCA+AGG 0.552218 1_3:+61994176 Msa0131240:CDS
CAACGTAACCGGCTGTAATG+AGG 0.557570 1_3:-61994839 None:intergenic
TGTGATGAGACGGGAAGAGA+AGG 0.558428 1_3:-61994032 None:intergenic
AAGGTTGGCTAACGAGAGGT+TGG 0.561612 1_3:+61996525 Msa0131240:CDS
ACGAGAGGTTGGCTACTTCG+CGG 0.563072 1_3:+61996536 Msa0131240:CDS
GGCTACTTCGCGGAGTGCAG+GGG 0.564830 1_3:+61996546 Msa0131240:CDS
ATGTACCAAATATAACAGTT+GGG 0.565063 1_3:-61995800 None:intergenic
GAGTTGCAAACAAATGCTGC+AGG 0.567919 1_3:+61996568 Msa0131240:CDS
AGAAAAGGTAGTTACCTGGC+GGG 0.583997 1_3:-61994276 None:intergenic
ATGAGCGACATTATCACAGT+TGG 0.585291 1_3:+61996650 Msa0131240:three_prime_UTR
CGTTGAACAAACTGTTCATG+GGG 0.600069 1_3:+61994857 Msa0131240:CDS
TGAGCCAGTATGGGGTCCCA+GGG 0.605388 1_3:+61995029 Msa0131240:CDS
CAGCTCAATTATCTGAGACA+AGG 0.610943 1_3:-61996767 None:intergenic
GGATTCACACGACTCTGCAA+GGG 0.617498 1_3:+61994177 Msa0131240:CDS
CGAGGTAGGTGAGAGCGGAA+GGG 0.618929 1_3:-61994245 None:intergenic
AAGTTGCTTGAGCCAGTATG+GGG 0.623617 1_3:+61995021 Msa0131240:CDS
TACCTGTGACTGTGATGAGA+CGG 0.626766 1_3:-61994042 None:intergenic
GGCGGGAATGAGAGCGAGGT+AGG 0.628802 1_3:-61994259 None:intergenic
ACCTGTGACTGTGATGAGAC+GGG 0.629877 1_3:-61994041 None:intergenic
TGTAATGTAGTACAAAGCAA+TGG 0.632081 1_3:-61995112 None:intergenic
ATATTGATTATACAACTGCA+TGG 0.632517 1_3:+61996809 Msa0131240:three_prime_UTR
CCATTGAGGCATCTAGGAGA+CGG 0.641159 1_3:+61996145 Msa0131240:CDS
AGAATGCAAAATCCTCACTT+GGG 0.648231 1_3:-61995884 None:intergenic
ACCTGGCGGGAATGAGAGCG+AGG 0.651805 1_3:-61994263 None:intergenic
GCTTAAGGGTGACCCAAGTG+AGG 0.669268 1_3:+61995872 Msa0131240:CDS
ATATTTCATCGAATGCTCTG+TGG 0.677191 1_3:+61996054 Msa0131240:CDS
AAGAACGTTATCGACTGTGT+CGG 0.685286 1_3:+61994149 Msa0131240:CDS
AAGAAAGGTTGGCTAACGAG+AGG 0.692597 1_3:+61996521 Msa0131240:CDS
GTCTCCTAGATGCCTCAATG+GGG 0.734529 1_3:-61996143 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTCATAATCTATTAATTAT+TGG - chr1_3:61994343-61994362 None:intergenic 15.0%
!! TTTAATAATTGTACTAAGTT+GGG - chr1_3:61994590-61994609 None:intergenic 15.0%
!!! TTTTAATAATTGTACTAAGT+TGG - chr1_3:61994591-61994610 None:intergenic 15.0%
!! TAAAAACATACTAATGTTAA+TGG - chr1_3:61994962-61994981 None:intergenic 15.0%
!!! TAACATTAGTATGTTTTTAT+AGG + chr1_3:61994963-61994982 Msa0131240:intron 15.0%
!! AATATAATCTCATAAAGTAA+TGG - chr1_3:61995170-61995189 None:intergenic 15.0%
!! GTAAAAAGATATAAAAGAAT+TGG - chr1_3:61995470-61995489 None:intergenic 15.0%
!! AAATTATAAATTACTACTGT+GGG + chr1_3:61996215-61996234 Msa0131240:intron 15.0%
!! CAAATTAAAATATTCCTTTA+AGG - chr1_3:61996704-61996723 None:intergenic 15.0%
!!! TATTTTAATTTGCATGTATT+TGG + chr1_3:61996712-61996731 Msa0131240:three_prime_UTR 15.0%
!! AGACAAATAAAAGAAAGAAT+TGG + chr1_3:61993967-61993986 Msa0131240:five_prime_UTR 20.0%
!! GACAAATAAAAGAAAGAATT+GGG + chr1_3:61993968-61993987 Msa0131240:five_prime_UTR 20.0%
!! TATTGGAAATATGTGATAAA+GGG - chr1_3:61994326-61994345 None:intergenic 20.0%
!! TTATTGGAAATATGTGATAA+AGG - chr1_3:61994327-61994346 None:intergenic 20.0%
!!! AAAGGAAAATAACTTGTATT+TGG - chr1_3:61994777-61994796 None:intergenic 20.0%
!! AAGAAAGAAATATGATAAAC+AGG - chr1_3:61995228-61995247 None:intergenic 20.0%
!! TAAGCTTGTAATAGAAAAAA+TGG - chr1_3:61995280-61995299 None:intergenic 20.0%
!!! TTTTTCTATTACAAGCTTAA+TGG + chr1_3:61995281-61995300 Msa0131240:intron 20.0%
!!! ATGACTTGTTTAAAAAACAT+TGG - chr1_3:61995370-61995389 None:intergenic 20.0%
!!! TTCATTTTTATATTGACGAA+AGG - chr1_3:61995591-61995610 None:intergenic 20.0%
!! TTTCGTCAATATAAAAATGA+AGG + chr1_3:61995590-61995609 Msa0131240:CDS 20.0%
!!! TTTAAGGTAAGTTATGATTT+TGG + chr1_3:61995926-61995945 Msa0131240:intron 20.0%
!! GACATCAGATATAAAAAAAA+AGG - chr1_3:61996003-61996022 None:intergenic 20.0%
!! CAAATTATAAATTACTACTG+TGG + chr1_3:61996214-61996233 Msa0131240:intron 20.0%
!! AATTATAAATTACTACTGTG+GGG + chr1_3:61996216-61996235 Msa0131240:intron 20.0%
!! ATCTGAATTTACAAAATCTA+GGG - chr1_3:61996606-61996625 None:intergenic 20.0%
!! TATCTGAATTTACAAAATCT+AGG - chr1_3:61996607-61996626 None:intergenic 20.0%
!! TTTGTAAATTCAGATAAGTT+TGG + chr1_3:61996611-61996630 Msa0131240:three_prime_UTR 20.0%
! AATTGAGAATTGCAATTTGT+AGG + chr1_3:61994122-61994141 Msa0131240:intron 25.0%
! ATTGAGAATTGCAATTTGTA+GGG + chr1_3:61994123-61994142 Msa0131240:intron 25.0%
! TTAGGTATAAGTATAACCAT+TGG + chr1_3:61994452-61994471 Msa0131240:intron 25.0%
! ATAATGTTATGAAGACTCAT+AGG - chr1_3:61994705-61994724 None:intergenic 25.0%
!! GAAAATAACTTGTATTTGGT+AGG - chr1_3:61994773-61994792 None:intergenic 25.0%
!! ATAGTTTGTCTTCTCTTAAT+TGG + chr1_3:61994997-61995016 Msa0131240:CDS 25.0%
! ATGAACTTTATAAATTCCCT+GGG - chr1_3:61995048-61995067 None:intergenic 25.0%
! ACATAATGAAAGCTTGAAAT+GGG - chr1_3:61995310-61995329 None:intergenic 25.0%
! ATGTACCAAATATAACAGTT+GGG - chr1_3:61995803-61995822 None:intergenic 25.0%
! TATGTACCAAATATAACAGT+TGG - chr1_3:61995804-61995823 None:intergenic 25.0%
! TAACTTACCTTAAAATCTCT+GGG - chr1_3:61995920-61995939 None:intergenic 25.0%
! ATAACTTACCTTAAAATCTC+TGG - chr1_3:61995921-61995940 None:intergenic 25.0%
!!! AATTTGCTATATACTCAACT+AGG - chr1_3:61996200-61996219 None:intergenic 25.0%
! AAATGGTTTCAGTTAAAGAA+AGG + chr1_3:61996322-61996341 Msa0131240:intron 25.0%
! CTCAAATAAACAAGAAAAGA+TGG - chr1_3:61996379-61996398 None:intergenic 25.0%
!!! ATTCTTTTGTTTGTCCTTAA+AGG + chr1_3:61996687-61996706 Msa0131240:three_prime_UTR 25.0%
!! ATATTGATTATACAACTGCA+TGG + chr1_3:61996809-61996828 Msa0131240:three_prime_UTR 25.0%
AATTGAAGGTGAGAAAGAAA+AGG - chr1_3:61994294-61994313 None:intergenic 30.0%
AAGGGAAACATTTGAATTGA+AGG - chr1_3:61994308-61994327 None:intergenic 30.0%
ACTTATACCTAAGCTAATGA+TGG - chr1_3:61994444-61994463 None:intergenic 30.0%
! CTGTTTTCATATATTGTCAC+CGG + chr1_3:61994499-61994518 Msa0131240:intron 30.0%
! AAAGTGTCTGTGTTTATAAG+TGG + chr1_3:61994643-61994662 Msa0131240:intron 30.0%
AAGTGTCTGTGTTTATAAGT+GGG + chr1_3:61994644-61994663 Msa0131240:intron 30.0%
! CATTATTTTCCGTCAATGTA+AGG + chr1_3:61994719-61994738 Msa0131240:intron 30.0%
!! AAGTTATTTTCCTTTCCTGT+TGG + chr1_3:61994782-61994801 Msa0131240:intron 30.0%
GATGAACTTTATAAATTCCC+TGG - chr1_3:61995049-61995068 None:intergenic 30.0%
TGTAATGTAGTACAAAGCAA+TGG - chr1_3:61995115-61995134 None:intergenic 30.0%
TTTGTACTACATTACAAGAC+AGG + chr1_3:61995119-61995138 Msa0131240:CDS 30.0%
TTCTATTACAAGCTTAATGG+AGG + chr1_3:61995284-61995303 Msa0131240:intron 30.0%
CACATAATGAAAGCTTGAAA+TGG - chr1_3:61995311-61995330 None:intergenic 30.0%
CATTTCAAGCTTTCATTATG+TGG + chr1_3:61995309-61995328 Msa0131240:intron 30.0%
TCAACACTAGAAAACATTGT+TGG + chr1_3:61995344-61995363 Msa0131240:intron 30.0%
ATTAATGTGCATTGTGCAAT+TGG - chr1_3:61995447-61995466 None:intergenic 30.0%
AAGCAATGAGTTACAAGTTA+AGG - chr1_3:61995636-61995655 None:intergenic 30.0%
ATTTACTTCACAGCAACAAT+AGG + chr1_3:61995657-61995676 Msa0131240:intron 30.0%
TATCTCCCAACTGTTATATT+TGG + chr1_3:61995795-61995814 Msa0131240:CDS 30.0%
TATTTGGTACATATATGAGC+TGG + chr1_3:61995811-61995830 Msa0131240:CDS 30.0%
! CATTTTTCCCAGAGATTTTA+AGG + chr1_3:61995910-61995929 Msa0131240:CDS 30.0%
AAGTTGCTCAGAATAACTAA+AGG - chr1_3:61995977-61995996 None:intergenic 30.0%
CGATGAAATATTGATGCAAT+AGG - chr1_3:61996045-61996064 None:intergenic 30.0%
! TGAATTAAAAGCGCAGAAAA+GGG - chr1_3:61996177-61996196 None:intergenic 30.0%
AGAAAGATAATAGAAGTTGC+AGG + chr1_3:61996261-61996280 Msa0131240:intron 30.0%
!!! GAAACTTTTTTTTGCTGTGA+TGG + chr1_3:61996287-61996306 Msa0131240:intron 30.0%
TATTACAACAATGCTATGAC+AGG - chr1_3:61996429-61996448 None:intergenic 30.0%
ATTGCTTATCGATCCAATAT+TGG + chr1_3:61996849-61996868 Msa0131240:three_prime_UTR 30.0%
TGATTCTTGACATCCAATAT+TGG - chr1_3:61996865-61996884 None:intergenic 30.0%
AGAAAGAAAAGGTAGTTACC+TGG - chr1_3:61994283-61994302 None:intergenic 35.0%
CAATATATGAAAACAGGAGC+AGG - chr1_3:61994495-61994514 None:intergenic 35.0%
CGGTGACAATATATGAAAAC+AGG - chr1_3:61994501-61994520 None:intergenic 35.0%
AGTGTCTGTGTTTATAAGTG+GGG + chr1_3:61994645-61994664 Msa0131240:intron 35.0%
ATGAAGACTCATAGGAATTG+TGG - chr1_3:61994697-61994716 None:intergenic 35.0%
!! AAGAAAGCACCTTACATTGA+CGG - chr1_3:61994731-61994750 None:intergenic 35.0%
! AACAGTTTGTTCAACGTAAC+CGG - chr1_3:61994853-61994872 None:intergenic 35.0%
TACGTTGAACAAACTGTTCA+TGG + chr1_3:61994855-61994874 Msa0131240:CDS 35.0%
ACGTTGAACAAACTGTTCAT+GGG + chr1_3:61994856-61994875 Msa0131240:CDS 35.0%
GTATAGCTAGAACATTGCAA+AGG - chr1_3:61995492-61995511 None:intergenic 35.0%
GAGTTACAAGTTAAGGAACA+AGG - chr1_3:61995629-61995648 None:intergenic 35.0%
GAGACATGGCACAATATTTA+GGG - chr1_3:61995697-61995716 None:intergenic 35.0%
AGAGACATGGCACAATATTT+AGG - chr1_3:61995698-61995717 None:intergenic 35.0%
GAACACAATAAAGCGATAGA+TGG - chr1_3:61995738-61995757 None:intergenic 35.0%
AGAATGCAAAATCCTCACTT+GGG - chr1_3:61995887-61995906 None:intergenic 35.0%
GATGCAATAGGATCTATTAC+TGG - chr1_3:61996033-61996052 None:intergenic 35.0%
ATATTTCATCGAATGCTCTG+TGG + chr1_3:61996054-61996073 Msa0131240:CDS 35.0%
! GTGAATTAAAAGCGCAGAAA+AGG - chr1_3:61996178-61996197 None:intergenic 35.0%
CAAGAGAAAAGTAGTCACAA+TGG - chr1_3:61996475-61996494 None:intergenic 35.0%
! CTTTTCTCTTGTAGAGAAAG+AGG + chr1_3:61996484-61996503 Msa0131240:intron 35.0%
CGCTCATGATAGTTTCATAT+TGG - chr1_3:61996637-61996656 None:intergenic 35.0%
TGATTATACAACTGCATGGA+AGG + chr1_3:61996813-61996832 Msa0131240:three_prime_UTR 35.0%
! AAGAACGTTATCGACTGTGT+CGG + chr1_3:61994149-61994168 Msa0131240:CDS 40.0%
AAGAAAAGGTAGTTACCTGG+CGG - chr1_3:61994280-61994299 None:intergenic 40.0%
! ATTGCTGCCATCATTAGCTT+AGG + chr1_3:61994434-61994453 Msa0131240:intron 40.0%
TGTGGCTTATGTAATGCCAA+TGG - chr1_3:61994471-61994490 None:intergenic 40.0%
TCCACTTGTCAGTGTTTCAT+AGG + chr1_3:61994524-61994543 Msa0131240:intron 40.0%
ACCTATGAAACACTGACAAG+TGG - chr1_3:61994528-61994547 None:intergenic 40.0%
TTGTCAGTGTTTCATAGGTG+TGG + chr1_3:61994529-61994548 Msa0131240:intron 40.0%
TGTCAGTGTTTCATAGGTGT+GGG + chr1_3:61994530-61994549 Msa0131240:intron 40.0%
! TGTACTAAGTTGGGAGTGAA+AGG - chr1_3:61994581-61994600 None:intergenic 40.0%
GTGTCTGTGTTTATAAGTGG+GGG + chr1_3:61994646-61994665 Msa0131240:intron 40.0%
GACTCATAGGAATTGTGGTT+GGG - chr1_3:61994692-61994711 None:intergenic 40.0%
AGACTCATAGGAATTGTGGT+TGG - chr1_3:61994693-61994712 None:intergenic 40.0%
CAACATGAAACCAACAGGAA+AGG - chr1_3:61994795-61994814 None:intergenic 40.0%
CGTTGAACAAACTGTTCATG+GGG + chr1_3:61994857-61994876 Msa0131240:CDS 40.0%
TGAAGCAACAGATAGATCTC+AGG - chr1_3:61994927-61994946 None:intergenic 40.0%
! GAGATCTATCTGTTGCTTCA+AGG + chr1_3:61994927-61994946 Msa0131240:intron 40.0%
GAGTTTCAAAGTGAAGTGCA+AGG - chr1_3:61995258-61995277 None:intergenic 40.0%
ACTCCATGAAATCCAGAAAG+TGG - chr1_3:61995516-61995535 None:intergenic 40.0%
GGATTTCATGGAGTGATATC+TGG + chr1_3:61995522-61995541 Msa0131240:CDS 40.0%
CACTGAACAGAAAAGAGACA+TGG - chr1_3:61995711-61995730 None:intergenic 40.0%
! CATGTCTCTTTTCTGTTCAG+TGG + chr1_3:61995709-61995728 Msa0131240:intron 40.0%
! GTTCTATTGTTCTGAGCTTC+TGG + chr1_3:61995754-61995773 Msa0131240:CDS 40.0%
TCTGAGCTTCTGGATAATAG+AGG + chr1_3:61995764-61995783 Msa0131240:CDS 40.0%
AAGCCGGAAACAAAGCTTAA+GGG + chr1_3:61995858-61995877 Msa0131240:CDS 40.0%
!! TCACCCTTAAGCTTTGTTTC+CGG - chr1_3:61995864-61995883 None:intergenic 40.0%
GAGAATGCAAAATCCTCACT+TGG - chr1_3:61995888-61995907 None:intergenic 40.0%
! AGGATCTATTACTGGTCTAC+AGG - chr1_3:61996025-61996044 None:intergenic 40.0%
GATGCTGAAGATTACGATCT+TGG + chr1_3:61996096-61996115 Msa0131240:CDS 40.0%
GAAGATTACGATCTTGGAAG+TGG + chr1_3:61996102-61996121 Msa0131240:CDS 40.0%
! GATGGTTGCTTGATGGTAAA+TGG + chr1_3:61996305-61996324 Msa0131240:intron 40.0%
ATGAGCGACATTATCACAGT+TGG + chr1_3:61996650-61996669 Msa0131240:three_prime_UTR 40.0%
! CAGCTCAATTATCTGAGACA+AGG - chr1_3:61996770-61996789 None:intergenic 40.0%
GATCATCATCATCATCGTCG+TGG + chr1_3:61993933-61993952 Msa0131240:five_prime_UTR 45.0%
TACCTGTGACTGTGATGAGA+CGG - chr1_3:61994045-61994064 None:intergenic 45.0%
AGAAAAGGTAGTTACCTGGC+GGG - chr1_3:61994279-61994298 None:intergenic 45.0%
ATGAAAACAGGAGCAGGTTG+TGG - chr1_3:61994489-61994508 None:intergenic 45.0%
AAACACTGACAAGTGGACAC+CGG - chr1_3:61994521-61994540 None:intergenic 45.0%
GTTTATAAGTGGGGGATTCG+TGG + chr1_3:61994654-61994673 Msa0131240:intron 45.0%
!! TCCTGTTGGTTTCATGTTGC+AGG + chr1_3:61994796-61994815 Msa0131240:intron 45.0%
TCCTGCAACATGAAACCAAC+AGG - chr1_3:61994800-61994819 None:intergenic 45.0%
GTAATGAGGTTCCAGGCAAA+TGG - chr1_3:61994828-61994847 None:intergenic 45.0%
CAAGTTGCTTGAGCCAGTAT+GGG + chr1_3:61995020-61995039 Msa0131240:CDS 45.0%
AAGTTGCTTGAGCCAGTATG+GGG + chr1_3:61995021-61995040 Msa0131240:CDS 45.0%
CAAAGTGAAGTGCAAGGTCT+GGG - chr1_3:61995252-61995271 None:intergenic 45.0%
TCAAAGTGAAGTGCAAGGTC+TGG - chr1_3:61995253-61995272 None:intergenic 45.0%
! TCTAGCTATACGCCACTTTC+TGG + chr1_3:61995501-61995520 Msa0131240:intron 45.0%
! ACGCCACTTTCTGGATTTCA+TGG + chr1_3:61995510-61995529 Msa0131240:CDS 45.0%
! TGGAGTGATATCTGGCTTCT+TGG + chr1_3:61995530-61995549 Msa0131240:CDS 45.0%
ACGAAAGGAAGCTCTTGATC+TGG - chr1_3:61995576-61995595 None:intergenic 45.0%
GCTGGATTTACCTGAGATAC+TGG + chr1_3:61995829-61995848 Msa0131240:CDS 45.0%
GAAGCCGGAAACAAAGCTTA+AGG + chr1_3:61995857-61995876 Msa0131240:CDS 45.0%
TTGCTGTGATGGTTGCTTGA+TGG + chr1_3:61996298-61996317 Msa0131240:intron 45.0%
! AAGAAAGGTTGGCTAACGAG+AGG + chr1_3:61996521-61996540 Msa0131240:CDS 45.0%
GAGTTGCAAACAAATGCTGC+AGG + chr1_3:61996568-61996587 Msa0131240:CDS 45.0%
GACTGTGATCTTCATGTGAG+TGG + chr1_3:61996734-61996753 Msa0131240:three_prime_UTR 45.0%
TGTGATGAGACGGGAAGAGA+AGG - chr1_3:61994035-61994054 None:intergenic 50.0%
ACCTGTGACTGTGATGAGAC+GGG - chr1_3:61994044-61994063 None:intergenic 50.0%
! TTATCGACTGTGTCGGTGTC+TGG + chr1_3:61994156-61994175 Msa0131240:CDS 50.0%
TGGATTCACACGACTCTGCA+AGG + chr1_3:61994176-61994195 Msa0131240:CDS 50.0%
GGATTCACACGACTCTGCAA+GGG + chr1_3:61994177-61994196 Msa0131240:CDS 50.0%
CAGTGTTTCATAGGTGTGGG+TGG + chr1_3:61994533-61994552 Msa0131240:intron 50.0%
TTTCATAGGTGTGGGTGGAG+TGG + chr1_3:61994538-61994557 Msa0131240:intron 50.0%
GCAGGACAATACCATTTGCC+TGG + chr1_3:61994814-61994833 Msa0131240:intron 50.0%
CAACGTAACCGGCTGTAATG+AGG - chr1_3:61994842-61994861 None:intergenic 50.0%
GCAAGTTGCTTGAGCCAGTA+TGG + chr1_3:61995019-61995038 Msa0131240:CDS 50.0%
GCTTCTTCTGCCAGTATCTC+AGG - chr1_3:61995842-61995861 None:intergenic 50.0%
GAGATACTGGCAGAAGAAGC+CGG + chr1_3:61995842-61995861 Msa0131240:CDS 50.0%
CTTGGAAGTGGACATTTGCC+AGG + chr1_3:61996114-61996133 Msa0131240:CDS 50.0%
GTCTCCTAGATGCCTCAATG+GGG - chr1_3:61996146-61996165 None:intergenic 50.0%
CGTCTCCTAGATGCCTCAAT+GGG - chr1_3:61996147-61996166 None:intergenic 50.0%
! CCATTGAGGCATCTAGGAGA+CGG + chr1_3:61996145-61996164 Msa0131240:CDS 50.0%
CAACCTTTCTTCCAGTGCTC+GGG - chr1_3:61996512-61996531 None:intergenic 50.0%
CCAACCTTTCTTCCAGTGCT+CGG - chr1_3:61996513-61996532 None:intergenic 50.0%
! AAGGTTGGCTAACGAGAGGT+TGG + chr1_3:61996525-61996544 Msa0131240:CDS 50.0%
TCCCGTCTCATCACAGTCAC+AGG + chr1_3:61994040-61994059 Msa0131240:CDS 55.0%
!! GGGATTCGTGGTGTTGCGTT+AGG + chr1_3:61994666-61994685 Msa0131240:intron 55.0%
GCCTGGAACCTCATTACAGC+CGG + chr1_3:61994831-61994850 Msa0131240:CDS 55.0%
ACCGGCTGTAATGAGGTTCC+AGG - chr1_3:61994835-61994854 None:intergenic 55.0%
AATTCCCTGGGACCCCATAC+TGG - chr1_3:61995036-61995055 None:intergenic 55.0%
GCTTAAGGGTGACCCAAGTG+AGG + chr1_3:61995872-61995891 Msa0131240:CDS 55.0%
! CTGACCCCATTGAGGCATCT+AGG + chr1_3:61996139-61996158 Msa0131240:CDS 55.0%
CCGTCTCCTAGATGCCTCAA+TGG - chr1_3:61996148-61996167 None:intergenic 55.0%
!! CCGAGCACTGGAAGAAAGGT+TGG + chr1_3:61996510-61996529 Msa0131240:CDS 55.0%
! ACGAGAGGTTGGCTACTTCG+CGG + chr1_3:61996536-61996555 Msa0131240:CDS 55.0%
CGAGGTAGGTGAGAGCGGAA+GGG - chr1_3:61994248-61994267 None:intergenic 60.0%
GAGAGCGAGGTAGGTGAGAG+CGG - chr1_3:61994253-61994272 None:intergenic 60.0%
TTGAGCCAGTATGGGGTCCC+AGG + chr1_3:61995028-61995047 Msa0131240:CDS 60.0%
TGAGCCAGTATGGGGTCCCA+GGG + chr1_3:61995029-61995048 Msa0131240:CDS 60.0%
! GCCAGGTTCTGACCCCATTG+AGG + chr1_3:61996131-61996150 Msa0131240:CDS 60.0%
GCCTCAATGGGGTCAGAACC+TGG - chr1_3:61996135-61996154 None:intergenic 60.0%
!! AGAAAGAGGTGCCCGAGCAC+TGG + chr1_3:61996498-61996517 Msa0131240:CDS 60.0%
!! GTGCCCGAGCACTGGAAGAA+AGG + chr1_3:61996506-61996525 Msa0131240:CDS 60.0%
TTGGCTACTTCGCGGAGTGC+AGG + chr1_3:61996544-61996563 Msa0131240:CDS 60.0%
TGGCTACTTCGCGGAGTGCA+GGG + chr1_3:61996545-61996564 Msa0131240:CDS 60.0%
GCGAGGTAGGTGAGAGCGGA+AGG - chr1_3:61994249-61994268 None:intergenic 65.0%
GGCGGGAATGAGAGCGAGGT+AGG - chr1_3:61994262-61994281 None:intergenic 65.0%
ACCTCGCTCTCATTCCCGCC+AGG + chr1_3:61994262-61994281 Msa0131240:CDS 65.0%
ACCTGGCGGGAATGAGAGCG+AGG - chr1_3:61994266-61994285 None:intergenic 65.0%
GGCTACTTCGCGGAGTGCAG+GGG + chr1_3:61996546-61996565 Msa0131240:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 61993904 61996899 61993904 ID=Msa0131240;Name=Msa0131240
chr1_3 mRNA 61993904 61996899 61993904 ID=Msa0131240-mRNA-1;Parent=Msa0131240;Name=Msa0131240-mRNA-1;_AED=0.15;_eAED=0.15;_QI=119|1|1|1|1|1|8|299|318
chr1_3 exon 61993904 61994061 61993904 ID=Msa0131240-mRNA-1:exon:20615;Parent=Msa0131240-mRNA-1
chr1_3 exon 61994144 61994283 61994144 ID=Msa0131240-mRNA-1:exon:20616;Parent=Msa0131240-mRNA-1
chr1_3 exon 61994818 61994878 61994818 ID=Msa0131240-mRNA-1:exon:20617;Parent=Msa0131240-mRNA-1
chr1_3 exon 61994985 61995151 61994985 ID=Msa0131240-mRNA-1:exon:20618;Parent=Msa0131240-mRNA-1
chr1_3 exon 61995506 61995611 61995506 ID=Msa0131240-mRNA-1:exon:20619;Parent=Msa0131240-mRNA-1
chr1_3 exon 61995729 61995931 61995729 ID=Msa0131240-mRNA-1:exon:20620;Parent=Msa0131240-mRNA-1
chr1_3 exon 61996029 61996166 61996029 ID=Msa0131240-mRNA-1:exon:20621;Parent=Msa0131240-mRNA-1
chr1_3 exon 61996498 61996899 61996498 ID=Msa0131240-mRNA-1:exon:20622;Parent=Msa0131240-mRNA-1
chr1_3 five_prime_UTR 61993904 61994022 61993904 ID=Msa0131240-mRNA-1:five_prime_utr;Parent=Msa0131240-mRNA-1
chr1_3 CDS 61994023 61994061 61994023 ID=Msa0131240-mRNA-1:cds;Parent=Msa0131240-mRNA-1
chr1_3 CDS 61994144 61994283 61994144 ID=Msa0131240-mRNA-1:cds;Parent=Msa0131240-mRNA-1
chr1_3 CDS 61994818 61994878 61994818 ID=Msa0131240-mRNA-1:cds;Parent=Msa0131240-mRNA-1
chr1_3 CDS 61994985 61995151 61994985 ID=Msa0131240-mRNA-1:cds;Parent=Msa0131240-mRNA-1
chr1_3 CDS 61995506 61995611 61995506 ID=Msa0131240-mRNA-1:cds;Parent=Msa0131240-mRNA-1
chr1_3 CDS 61995729 61995931 61995729 ID=Msa0131240-mRNA-1:cds;Parent=Msa0131240-mRNA-1
chr1_3 CDS 61996029 61996166 61996029 ID=Msa0131240-mRNA-1:cds;Parent=Msa0131240-mRNA-1
chr1_3 CDS 61996498 61996600 61996498 ID=Msa0131240-mRNA-1:cds;Parent=Msa0131240-mRNA-1
chr1_3 three_prime_UTR 61996601 61996899 61996601 ID=Msa0131240-mRNA-1:three_prime_utr;Parent=Msa0131240-mRNA-1
Gene Sequence

>Msa0131240

ATGAGCTCGCCTTCTCTTCCCGTCTCATCACAGTCACAGGGCACAAGAACGTTATCGACTGTGTCGGTGTCTGGATTCACACGACTCTGCAAGGGTCTCGCTCTCGTTCTCGTTGCAGCTCACGCTGTTCTTTATTTCTTCCCTTCCGCTCTCACCTACCTCGCTCTCATTCCCGCCAGGACAATACCATTTGCCTGGAACCTCATTACAGCCGGTTACGTTGAACAAACTGTTCATGGGGTTGTTATCAGCATAGTTTGTCTTCTCTTAATTGGCAAGTTGCTTGAGCCAGTATGGGGTCCCAGGGAATTTATAAAGTTCATCTTTATAGTTAACATCTTAACTTCTCTCTGCATTTTCATCACTGCCATTGCTTTGTACTACATTACAAGACAGGAGATTTATCTCTATACGCCACTTTCTGGATTTCATGGAGTGATATCTGGCTTCTTGGTTAGCATCAAGCAAATTATACCAGATCAAGAGCTTCCTTTCGTCAATATAAAAATGAAGTGGCTACCATCTATCGCTTTATTGTGTTCTATTGTTCTGAGCTTCTGGATAATAGAGGCAACAACATATCTCCCAACTGTTATATTTGGTACATATATGAGCTGGATTTACCTGAGATACTGGCAGAAGAAGCCGGAAACAAAGCTTAAGGGTGACCCAAGTGAGGATTTTGCATTCTCTACATTTTTCCCAGAGATTTTAAGACCAGTAATAGATCCTATTGCATCAATATTTCATCGAATGCTCTGTGGAAGATCTGATGCTTCTAATGATGCTGAAGATTACGATCTTGGAAGTGGACATTTGCCAGGTTCTGACCCCATTGAGGCATCTAGGAGACGAGAAAGAGGTGCCCGAGCACTGGAAGAAAGGTTGGCTAACGAGAGGTTGGCTACTTCGCGGAGTGCAGGGGAGTTGCAAACAAATGCTGCAGGAAATGTATAA
Protein sequence

>Msa0131240

MSSPSLPVSSQSQGTRTLSTVSVSGFTRLCKGLALVLVAAHAVLYFFPSALTYLALIPARTIPFAWNLITAGYVEQTVHGVVISIVCLLLIGKLLEPVWGPREFIKFIFIVNILTSLCIFITAIALYYITRQEIYLYTPLSGFHGVISGFLVSIKQIIPDQELPFVNIKMKWLPSIALLCSIVLSFWIIEATTYLPTVIFGTYMSWIYLRYWQKKPETKLKGDPSEDFAFSTFFPEILRPVIDPIASIFHRMLCGRSDASNDAEDYDLGSGHLPGSDPIEASRRRERGARALEERLANERLATSRSAGELQTNAAGNV*