AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0132390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0132390 MtrunA17_Chr4g0036071 76.812 69 16 0 1 69 334 402 5.76e-28 105
Msa0132390 MtrunA17_Chr2g0306581 81.250 48 9 0 15 62 1 48 1.75e-20 79.0
Msa0132390 MtrunA17_Chr1g0156051 69.492 59 18 0 1 59 361 419 8.07e-20 82.0
Msa0132390 MtrunA17_Chr2g0329551 61.765 68 26 0 2 69 196 263 1.74e-19 80.9
Msa0132390 MtrunA17_Chr5g0432701 66.667 60 20 0 10 69 142 201 1.31e-18 78.2
Msa0132390 MtrunA17_Chr3g0107491 66.667 60 20 0 10 69 160 219 1.35e-18 78.2
Msa0132390 MtrunA17_Chr6g0463571 70.909 55 16 0 11 65 135 189 3.04e-18 77.0
Msa0132390 MtrunA17_Chr3g0128781 60.000 60 24 0 10 69 91 150 4.20e-17 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0132390 AT4G38900.2 73.846 65 17 0 5 69 372 436 8.16e-24 94.0
Msa0132390 AT4G38900.3 73.846 65 17 0 5 69 372 436 8.16e-24 94.0
Msa0132390 AT2G21230.4 68.116 69 22 0 1 69 344 412 8.94e-24 93.6
Msa0132390 AT2G21230.1 68.116 69 22 0 1 69 344 412 8.94e-24 93.6
Msa0132390 AT2G21230.3 68.116 69 22 0 1 69 344 412 9.23e-24 93.6
Msa0132390 AT2G21230.2 69.118 68 21 0 2 69 345 412 1.04e-22 90.5
Msa0132390 AT4G38900.1 67.606 71 17 1 5 69 372 442 1.41e-21 87.4
Msa0132390 AT2G40620.1 70.000 60 18 0 10 69 131 190 1.97e-20 83.6
Msa0132390 AT2G31370.5 60.294 68 27 0 2 69 176 243 6.61e-19 79.7
Msa0132390 AT2G31370.2 60.294 68 27 0 2 69 176 243 6.91e-19 79.7
Msa0132390 AT2G31370.4 60.294 68 27 0 2 69 176 243 6.91e-19 79.7
Msa0132390 AT2G31370.1 60.294 68 27 0 2 69 176 243 6.91e-19 79.7
Msa0132390 AT2G31370.7 60.294 68 27 0 2 69 176 243 6.91e-19 79.7
Msa0132390 AT2G31370.3 60.294 68 27 0 2 69 176 243 6.91e-19 79.7
Msa0132390 AT2G31370.6 60.294 68 27 0 2 69 176 243 6.91e-19 79.7
Msa0132390 AT1G06850.2 65.000 60 21 0 10 69 131 190 5.58e-18 75.5
Msa0132390 AT1G06070.1 57.353 68 29 0 2 69 185 252 1.03e-17 76.6
Msa0132390 AT1G06850.1 65.000 60 21 0 10 69 131 190 1.32e-17 75.9
Msa0132390 AT1G43700.1 63.636 55 20 0 15 69 182 236 6.04e-16 71.2

Find 59 sgRNAs with CRISPR-Local

Find 276 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGGTTATTTCAGGATTT+TGG 0.101232 1_3:+63407917 Msa0132390:intron
GGACCTTAGCAAACTGTATT+TGG 0.168656 1_3:+63408980 Msa0132390:three_prime_UTR
GCTTAAGATGCGATATATTT+TGG 0.170519 1_3:+63407971 Msa0132390:CDS
ATTTGGAAAATAAATTTATA+AGG 0.205498 1_3:+63408997 Msa0132390:three_prime_UTR
AATAAATTTATAAGGGGAAT+TGG 0.239221 1_3:+63409005 Msa0132390:three_prime_UTR
TGCTACCAACACTGCTTTCT+CGG 0.291207 1_3:-63408112 None:intergenic
TTCAGAGTTGAAGAAAATTA+TGG 0.315927 1_3:+63407769 Msa0132390:CDS
TTTGGAAAATAAATTTATAA+GGG 0.316986 1_3:+63408998 Msa0132390:three_prime_UTR
AGGTTTGGCAACTTTGAGTA+TGG 0.321602 1_3:+63408666 Msa0132390:three_prime_UTR
GCAACGACACAACGAATTCT+TGG 0.332774 1_3:-63408224 None:intergenic
AACACTGCTTTCTCGGTTCT+AGG 0.357024 1_3:-63408105 None:intergenic
GGACTGTAATCTTGAACTTC+AGG 0.367870 1_3:-63408284 None:intergenic
GAGGTGTCATATTCTGAAAT+TGG 0.368658 1_3:-63408043 None:intergenic
ATAAATTTATAAGGGGAATT+GGG 0.372298 1_3:+63409006 Msa0132390:three_prime_UTR
GTTCTGAAATGCCTTCAGTT+TGG 0.388967 1_3:+63409097 Msa0132390:three_prime_UTR
AAAATAATACCTGGCCTTTG+TGG 0.394261 1_3:-63408323 None:intergenic
ATCTAATAATAATAATCAAC+TGG 0.395377 1_3:-63408895 None:intergenic
GAAAGTTGCTGAGATTCTTA+TGG 0.396164 1_3:+63407799 Msa0132390:CDS
ATTGAAGGTCTTGTTGTGTT+TGG 0.431019 1_3:-63408798 None:intergenic
GTTAACTTTCGCCACCGTAC+GGG 0.448129 1_3:+63408695 Msa0132390:three_prime_UTR
CAGCACCTCTTAGCCCGCTT+CGG 0.449837 1_3:-63407827 None:intergenic
CTATTCAGTTCCTTTGTGAA+TGG 0.454434 1_3:+63408765 Msa0132390:three_prime_UTR
TTGTGGAACCAAGCTCCATA+TGG 0.454668 1_3:-63408306 None:intergenic
TGTTAACTTTCGCCACCGTA+CGG 0.456246 1_3:+63408694 Msa0132390:three_prime_UTR
AATAGATGCAGTGCTCTAGA+AGG 0.460695 1_3:-63408747 None:intergenic
ATATCGCATCTTAAGCTCAA+TGG 0.469206 1_3:-63407964 None:intergenic
TTCAAGATTACAGTCCCATA+TGG 0.472759 1_3:+63408291 Msa0132390:three_prime_UTR
GAATTCGTTGTGTCGTTGCT+AGG 0.487493 1_3:+63408228 Msa0132390:three_prime_UTR
ACAACAAGACCTTCAATTGA+AGG 0.487972 1_3:+63408804 Msa0132390:three_prime_UTR
CTATGTGTAGGATGGAGGTT+TGG 0.488964 1_3:+63408651 Msa0132390:intron
GCTCTAGAAGGTTGTGCATA+GGG 0.490885 1_3:-63408735 None:intergenic
AAATTGTGTAAAGAACAATA+TGG 0.499131 1_3:-63409074 None:intergenic
TCAGTTCCTTTGTGAATGGT+AGG 0.502741 1_3:+63408769 Msa0132390:three_prime_UTR
TTCCACATGTTATATACAGT+TGG 0.506432 1_3:-63408855 None:intergenic
TTACAGTCCCATATGGAGCT+TGG 0.523649 1_3:+63408298 Msa0132390:three_prime_UTR
TTTCCAAATACAGTTTGCTA+AGG 0.524653 1_3:-63408983 None:intergenic
CTGATCCGAAGCGGGCTAAG+AGG 0.529672 1_3:+63407822 Msa0132390:CDS
TGTGGAACCAAGCTCCATAT+GGG 0.530718 1_3:-63408305 None:intergenic
GTTCAGACTCTGAAGACTGA+AGG 0.542615 1_3:+63408008 Msa0132390:CDS
TGCTCTAGAAGGTTGTGCAT+AGG 0.543792 1_3:-63408736 None:intergenic
TTGGAAAATAAATTTATAAG+GGG 0.545493 1_3:+63408999 Msa0132390:three_prime_UTR
TCTTATGGCTGATCCGAAGC+GGG 0.549131 1_3:+63407814 Msa0132390:CDS
ATTGCTGAAGCAAACTCAGC+AGG 0.554785 1_3:-63408067 None:intergenic
CATATCTATGTGTAGGATGG+AGG 0.564335 1_3:+63408646 Msa0132390:intron
CATGTGAGCTGCTCCCCGTA+CGG 0.564411 1_3:-63408709 None:intergenic
TAGAACCGAGAAAGCAGTGT+TGG 0.572911 1_3:+63408107 Msa0132390:exon
AGCTTGGTTCCACAAAGGCC+AGG 0.580658 1_3:+63408314 Msa0132390:three_prime_UTR
TTCAGACTCTGAAGACTGAA+GGG 0.583079 1_3:+63408009 Msa0132390:CDS
TTAACTTTCGCCACCGTACG+GGG 0.586054 1_3:+63408696 Msa0132390:three_prime_UTR
CAAGACCTTCAATTGAAGGA+TGG 0.592333 1_3:+63408808 Msa0132390:three_prime_UTR
TGCCAACTGTATATAACATG+TGG 0.599624 1_3:+63408853 Msa0132390:three_prime_UTR
CGAAACATAACAGATGGGAC+AGG 0.602460 1_3:+63406968 None:intergenic
CGTACGGGGAGCAGCTCACA+TGG 0.603251 1_3:+63408710 Msa0132390:three_prime_UTR
TGAAGCAAACTCAGCAGGAG+AGG 0.607777 1_3:-63408062 None:intergenic
CGAAAACTGATCCAAACTGA+AGG 0.608245 1_3:-63409108 None:intergenic
TTCTTATGGCTGATCCGAAG+CGG 0.620106 1_3:+63407813 Msa0132390:CDS
GTGAGCTGCTCCCCGTACGG+TGG 0.624321 1_3:-63408706 None:intergenic
TATGGAGCTTGGTTCCACAA+AGG 0.680414 1_3:+63408309 Msa0132390:three_prime_UTR
CAATCTCCTACCATTCACAA+AGG 0.682616 1_3:-63408775 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAATCTACTTTTTTTT+TGG - chr1_3:63407224-63407243 None:intergenic 10.0%
!! TAAAAAAAAAAAATAATACC+TGG - chr1_3:63408335-63408354 None:intergenic 10.0%
!! ATTTGGAAAATAAATTTATA+AGG + chr1_3:63408997-63409016 Msa0132390:three_prime_UTR 10.0%
!! TTTGGAAAATAAATTTATAA+GGG + chr1_3:63408998-63409017 Msa0132390:three_prime_UTR 10.0%
!! ATCATAAAAAATAAAAAGAA+TGG - chr1_3:63409045-63409064 None:intergenic 10.0%
!!! AAAAAAATCTACTTTTTTTT+TGG - chr1_3:63407224-63407243 None:intergenic 10.0%
!! TAAAAAAAAAAAATAATACC+TGG - chr1_3:63408335-63408354 None:intergenic 10.0%
!! ATTTGGAAAATAAATTTATA+AGG + chr1_3:63408997-63409016 Msa0132390:three_prime_UTR 10.0%
!! TTTGGAAAATAAATTTATAA+GGG + chr1_3:63408998-63409017 Msa0132390:three_prime_UTR 10.0%
!! ATCATAAAAAATAAAAAGAA+TGG - chr1_3:63409045-63409064 None:intergenic 10.0%
!! AATAAAAAATCTCAAGAAAA+AGG - chr1_3:63407093-63407112 None:intergenic 15.0%
!!! TTTTCTTGAGATTTTTTATT+TGG + chr1_3:63407093-63407112 Msa0132390:intron 15.0%
!! TACCATTATAAATGATAATT+TGG + chr1_3:63407262-63407281 Msa0132390:intron 15.0%
!! TTCCAAATTATCATTTATAA+TGG - chr1_3:63407267-63407286 None:intergenic 15.0%
!! ATTATAAATGATAATTTGGA+AGG + chr1_3:63407266-63407285 Msa0132390:intron 15.0%
!!! AATTATGTTTTGACTAAATT+AGG + chr1_3:63407343-63407362 Msa0132390:intron 15.0%
!!! TCTTTGTTTTTAAATTGAAT+GGG + chr1_3:63407409-63407428 Msa0132390:intron 15.0%
!! AAAAACATTTAAACTTCATA+AGG - chr1_3:63407465-63407484 None:intergenic 15.0%
!!! ATTTAACTTGGATATTTTTT+TGG + chr1_3:63407899-63407918 Msa0132390:intron 15.0%
!!! TTTAACTTGGATATTTTTTT+GGG + chr1_3:63407900-63407919 Msa0132390:intron 15.0%
!! ATCTAATAATAATAATCAAC+TGG - chr1_3:63408898-63408917 None:intergenic 15.0%
!! TTGGAAAATAAATTTATAAG+GGG + chr1_3:63408999-63409018 Msa0132390:three_prime_UTR 15.0%
!! AATAAAAAATCTCAAGAAAA+AGG - chr1_3:63407093-63407112 None:intergenic 15.0%
!!! TTTTCTTGAGATTTTTTATT+TGG + chr1_3:63407093-63407112 Msa0132390:intron 15.0%
!! TACCATTATAAATGATAATT+TGG + chr1_3:63407262-63407281 Msa0132390:intron 15.0%
!! TTCCAAATTATCATTTATAA+TGG - chr1_3:63407267-63407286 None:intergenic 15.0%
!! ATTATAAATGATAATTTGGA+AGG + chr1_3:63407266-63407285 Msa0132390:intron 15.0%
!!! AATTATGTTTTGACTAAATT+AGG + chr1_3:63407343-63407362 Msa0132390:intron 15.0%
!!! TCTTTGTTTTTAAATTGAAT+GGG + chr1_3:63407409-63407428 Msa0132390:intron 15.0%
!! AAAAACATTTAAACTTCATA+AGG - chr1_3:63407465-63407484 None:intergenic 15.0%
!!! ATTTAACTTGGATATTTTTT+TGG + chr1_3:63407899-63407918 Msa0132390:intron 15.0%
!!! TTTAACTTGGATATTTTTTT+GGG + chr1_3:63407900-63407919 Msa0132390:intron 15.0%
!! ATCTAATAATAATAATCAAC+TGG - chr1_3:63408898-63408917 None:intergenic 15.0%
!! TTGGAAAATAAATTTATAAG+GGG + chr1_3:63408999-63409018 Msa0132390:three_prime_UTR 15.0%
!! AACAAAGACTACAAATTAAT+AGG - chr1_3:63407025-63407044 None:intergenic 20.0%
!!! GTCTTTGTTTTTAAATTGAA+TGG + chr1_3:63407408-63407427 Msa0132390:intron 20.0%
!!! TTGTGATTTATGTGTATTTT+AGG + chr1_3:63407483-63407502 Msa0132390:intron 20.0%
!!! TTTATGTGTATTTTAGGATA+AGG + chr1_3:63407489-63407508 Msa0132390:intron 20.0%
!! TTTAGTTAGTAATGTCAATA+TGG + chr1_3:63407869-63407888 Msa0132390:intron 20.0%
!!! ATATTTTTTTGGGTTATTTC+AGG + chr1_3:63407910-63407929 Msa0132390:intron 20.0%
!! AGAAATGATCAATAAAGATT+TGG - chr1_3:63408144-63408163 None:intergenic 20.0%
!! GTGTTAAATTATTATGATAG+CGG + chr1_3:63408198-63408217 Msa0132390:intron 20.0%
!!! AAATTGTTAAAGAGTTTTGT+AGG + chr1_3:63408959-63408978 Msa0132390:three_prime_UTR 20.0%
!! AATAAATTTATAAGGGGAAT+TGG + chr1_3:63409005-63409024 Msa0132390:three_prime_UTR 20.0%
!! ATAAATTTATAAGGGGAATT+GGG + chr1_3:63409006-63409025 Msa0132390:three_prime_UTR 20.0%
!! AAATTGTGTAAAGAACAATA+TGG - chr1_3:63409077-63409096 None:intergenic 20.0%
!! AACAAAGACTACAAATTAAT+AGG - chr1_3:63407025-63407044 None:intergenic 20.0%
!!! GTCTTTGTTTTTAAATTGAA+TGG + chr1_3:63407408-63407427 Msa0132390:intron 20.0%
!!! TTGTGATTTATGTGTATTTT+AGG + chr1_3:63407483-63407502 Msa0132390:intron 20.0%
!!! TTTATGTGTATTTTAGGATA+AGG + chr1_3:63407489-63407508 Msa0132390:intron 20.0%
!! TTTAGTTAGTAATGTCAATA+TGG + chr1_3:63407869-63407888 Msa0132390:intron 20.0%
!!! ATATTTTTTTGGGTTATTTC+AGG + chr1_3:63407910-63407929 Msa0132390:intron 20.0%
!! AGAAATGATCAATAAAGATT+TGG - chr1_3:63408144-63408163 None:intergenic 20.0%
!! GTGTTAAATTATTATGATAG+CGG + chr1_3:63408198-63408217 Msa0132390:intron 20.0%
!!! AAATTGTTAAAGAGTTTTGT+AGG + chr1_3:63408959-63408978 Msa0132390:three_prime_UTR 20.0%
!! AATAAATTTATAAGGGGAAT+TGG + chr1_3:63409005-63409024 Msa0132390:three_prime_UTR 20.0%
!! ATAAATTTATAAGGGGAATT+GGG + chr1_3:63409006-63409025 Msa0132390:three_prime_UTR 20.0%
!! AAATTGTGTAAAGAACAATA+TGG - chr1_3:63409077-63409096 None:intergenic 20.0%
! CTAAATTACAATCTGTCATT+TGG + chr1_3:63407058-63407077 Msa0132390:intron 25.0%
!!! GGTATTGTTCTTTTTAACTA+TGG + chr1_3:63407114-63407133 Msa0132390:intron 25.0%
!!! CATTTTTTTTGACTTCATGT+TGG + chr1_3:63407149-63407168 Msa0132390:intron 25.0%
!!! AATCTACTTTTTTTTTGGCT+AGG - chr1_3:63407219-63407238 None:intergenic 25.0%
! ATAAATGATAATTTGGAAGG+AGG + chr1_3:63407269-63407288 Msa0132390:intron 25.0%
! ATATGTATCTTGACTCTATT+TGG + chr1_3:63407386-63407405 Msa0132390:intron 25.0%
!!! TTTACTGCTCTTATTTTACT+TGG + chr1_3:63407622-63407641 Msa0132390:intron 25.0%
! CATCAAAATTAGTTCAAATC+AGG - chr1_3:63407665-63407684 None:intergenic 25.0%
!!! TTGAACTAATTTTGATGTCT+TGG + chr1_3:63407668-63407687 Msa0132390:intron 25.0%
! TTCAGAGTTGAAGAAAATTA+TGG + chr1_3:63407769-63407788 Msa0132390:CDS 25.0%
! TATGGATGGTTAATTTAACT+TGG + chr1_3:63407887-63407906 Msa0132390:intron 25.0%
!! ATTAGCTTCTTAGTAATCAA+AGG - chr1_3:63408175-63408194 None:intergenic 25.0%
!!! ATTTTTCGAAATGCAGAAAA+TGG - chr1_3:63408367-63408386 None:intergenic 25.0%
! AAGTTCAGTATTCTATAGTA+TGG + chr1_3:63408387-63408406 Msa0132390:intron 25.0%
!!! GTATGGTTATGCAATTTTTT+CGG + chr1_3:63408404-63408423 Msa0132390:intron 25.0%
! ATTGAAGGATGGAAAAAAAA+TGG + chr1_3:63408819-63408838 Msa0132390:three_prime_UTR 25.0%
! TTGAAGGATGGAAAAAAAAT+GGG + chr1_3:63408820-63408839 Msa0132390:three_prime_UTR 25.0%
!!! ATTTTAAGAATGCTGTTGAT+TGG - chr1_3:63409141-63409160 None:intergenic 25.0%
! CTAAATTACAATCTGTCATT+TGG + chr1_3:63407058-63407077 Msa0132390:intron 25.0%
!!! GGTATTGTTCTTTTTAACTA+TGG + chr1_3:63407114-63407133 Msa0132390:intron 25.0%
!!! CATTTTTTTTGACTTCATGT+TGG + chr1_3:63407149-63407168 Msa0132390:intron 25.0%
!!! AATCTACTTTTTTTTTGGCT+AGG - chr1_3:63407219-63407238 None:intergenic 25.0%
! ATAAATGATAATTTGGAAGG+AGG + chr1_3:63407269-63407288 Msa0132390:intron 25.0%
! ATATGTATCTTGACTCTATT+TGG + chr1_3:63407386-63407405 Msa0132390:intron 25.0%
!!! TTTACTGCTCTTATTTTACT+TGG + chr1_3:63407622-63407641 Msa0132390:intron 25.0%
! CATCAAAATTAGTTCAAATC+AGG - chr1_3:63407665-63407684 None:intergenic 25.0%
!!! TTGAACTAATTTTGATGTCT+TGG + chr1_3:63407668-63407687 Msa0132390:intron 25.0%
! TTCAGAGTTGAAGAAAATTA+TGG + chr1_3:63407769-63407788 Msa0132390:CDS 25.0%
! TATGGATGGTTAATTTAACT+TGG + chr1_3:63407887-63407906 Msa0132390:intron 25.0%
!! ATTAGCTTCTTAGTAATCAA+AGG - chr1_3:63408175-63408194 None:intergenic 25.0%
!!! ATTTTTCGAAATGCAGAAAA+TGG - chr1_3:63408367-63408386 None:intergenic 25.0%
! AAGTTCAGTATTCTATAGTA+TGG + chr1_3:63408387-63408406 Msa0132390:intron 25.0%
!!! GTATGGTTATGCAATTTTTT+CGG + chr1_3:63408404-63408423 Msa0132390:intron 25.0%
! ATTGAAGGATGGAAAAAAAA+TGG + chr1_3:63408819-63408838 Msa0132390:three_prime_UTR 25.0%
! TTGAAGGATGGAAAAAAAAT+GGG + chr1_3:63408820-63408839 Msa0132390:three_prime_UTR 25.0%
!!! ATTTTAAGAATGCTGTTGAT+TGG - chr1_3:63409141-63409160 None:intergenic 25.0%
TTGTAATTTAGATGAGGCAT+GGG - chr1_3:63407049-63407068 None:intergenic 30.0%
ATTGTAATTTAGATGAGGCA+TGG - chr1_3:63407050-63407069 None:intergenic 30.0%
GACAGATTGTAATTTAGATG+AGG - chr1_3:63407055-63407074 None:intergenic 30.0%
! GAGTCAAGATACATATTTTG+CGG - chr1_3:63407382-63407401 None:intergenic 30.0%
ACAATGTGTGCTTACAATAT+GGG + chr1_3:63407559-63407578 Msa0132390:intron 30.0%
GTTAGTAATGTCAATATGGA+TGG + chr1_3:63407873-63407892 Msa0132390:intron 30.0%
!! TTTGGGTTATTTCAGGATTT+TGG + chr1_3:63407917-63407936 Msa0132390:intron 30.0%
! GCTTAAGATGCGATATATTT+TGG + chr1_3:63407971-63407990 Msa0132390:CDS 30.0%
TTGTCACTGTTGTCAAATAA+AGG + chr1_3:63408454-63408473 Msa0132390:intron 30.0%
TTGTCAAATAAAGGCTATAG+TGG + chr1_3:63408463-63408482 Msa0132390:intron 30.0%
! TTTTTCCATCCTTCAATTGA+AGG - chr1_3:63408816-63408835 None:intergenic 30.0%
TTCCACATGTTATATACAGT+TGG - chr1_3:63408858-63408877 None:intergenic 30.0%
!! TTTCCAAATACAGTTTGCTA+AGG - chr1_3:63408986-63409005 None:intergenic 30.0%
TTGTAATTTAGATGAGGCAT+GGG - chr1_3:63407049-63407068 None:intergenic 30.0%
ATTGTAATTTAGATGAGGCA+TGG - chr1_3:63407050-63407069 None:intergenic 30.0%
GACAGATTGTAATTTAGATG+AGG - chr1_3:63407055-63407074 None:intergenic 30.0%
! GAGTCAAGATACATATTTTG+CGG - chr1_3:63407382-63407401 None:intergenic 30.0%
ACAATGTGTGCTTACAATAT+GGG + chr1_3:63407559-63407578 Msa0132390:intron 30.0%
GTTAGTAATGTCAATATGGA+TGG + chr1_3:63407873-63407892 Msa0132390:intron 30.0%
!! TTTGGGTTATTTCAGGATTT+TGG + chr1_3:63407917-63407936 Msa0132390:intron 30.0%
! GCTTAAGATGCGATATATTT+TGG + chr1_3:63407971-63407990 Msa0132390:CDS 30.0%
TTGTCACTGTTGTCAAATAA+AGG + chr1_3:63408454-63408473 Msa0132390:intron 30.0%
TTGTCAAATAAAGGCTATAG+TGG + chr1_3:63408463-63408482 Msa0132390:intron 30.0%
! TTTTTCCATCCTTCAATTGA+AGG - chr1_3:63408816-63408835 None:intergenic 30.0%
TTCCACATGTTATATACAGT+TGG - chr1_3:63408858-63408877 None:intergenic 30.0%
!! TTTCCAAATACAGTTTGCTA+AGG - chr1_3:63408986-63409005 None:intergenic 30.0%
TGTAATTTAGATGAGGCATG+GGG - chr1_3:63407048-63407067 None:intergenic 35.0%
ATGTCTAAGCTATAACTCCT+TGG + chr1_3:63407183-63407202 Msa0132390:intron 35.0%
!! GGGAAGTACTTTGAGATATT+TGG + chr1_3:63407429-63407448 Msa0132390:intron 35.0%
GACAATGTGTGCTTACAATA+TGG + chr1_3:63407558-63407577 Msa0132390:intron 35.0%
GAAAGTTGCTGAGATTCTTA+TGG + chr1_3:63407799-63407818 Msa0132390:CDS 35.0%
!!! ATTTCAGGATTTTGGCTAAC+AGG + chr1_3:63407925-63407944 Msa0132390:intron 35.0%
ATATCGCATCTTAAGCTCAA+TGG - chr1_3:63407967-63407986 None:intergenic 35.0%
!!! TATTTTGGAGCTAGAACACA+AGG + chr1_3:63407986-63408005 Msa0132390:CDS 35.0%
!! GAGGTGTCATATTCTGAAAT+TGG - chr1_3:63408046-63408065 None:intergenic 35.0%
TTCAAGATTACAGTCCCATA+TGG + chr1_3:63408291-63408310 Msa0132390:three_prime_UTR 35.0%
! AAAATAATACCTGGCCTTTG+TGG - chr1_3:63408326-63408345 None:intergenic 35.0%
TGCATTGCATATCTATGTGT+AGG + chr1_3:63408639-63408658 Msa0132390:intron 35.0%
TTGCATATCTATGTGTAGGA+TGG + chr1_3:63408643-63408662 Msa0132390:intron 35.0%
CTATTCAGTTCCTTTGTGAA+TGG + chr1_3:63408765-63408784 Msa0132390:three_prime_UTR 35.0%
!! ATTGAAGGTCTTGTTGTGTT+TGG - chr1_3:63408801-63408820 None:intergenic 35.0%
ACAACAAGACCTTCAATTGA+AGG + chr1_3:63408804-63408823 Msa0132390:three_prime_UTR 35.0%
TGCCAACTGTATATAACATG+TGG + chr1_3:63408853-63408872 Msa0132390:three_prime_UTR 35.0%
TGTAATTTAGATGAGGCATG+GGG - chr1_3:63407048-63407067 None:intergenic 35.0%
ATGTCTAAGCTATAACTCCT+TGG + chr1_3:63407183-63407202 Msa0132390:intron 35.0%
!! GGGAAGTACTTTGAGATATT+TGG + chr1_3:63407429-63407448 Msa0132390:intron 35.0%
GACAATGTGTGCTTACAATA+TGG + chr1_3:63407558-63407577 Msa0132390:intron 35.0%
GAAAGTTGCTGAGATTCTTA+TGG + chr1_3:63407799-63407818 Msa0132390:CDS 35.0%
!!! ATTTCAGGATTTTGGCTAAC+AGG + chr1_3:63407925-63407944 Msa0132390:intron 35.0%
ATATCGCATCTTAAGCTCAA+TGG - chr1_3:63407967-63407986 None:intergenic 35.0%
!!! TATTTTGGAGCTAGAACACA+AGG + chr1_3:63407986-63408005 Msa0132390:CDS 35.0%
!! GAGGTGTCATATTCTGAAAT+TGG - chr1_3:63408046-63408065 None:intergenic 35.0%
TTCAAGATTACAGTCCCATA+TGG + chr1_3:63408291-63408310 Msa0132390:three_prime_UTR 35.0%
! AAAATAATACCTGGCCTTTG+TGG - chr1_3:63408326-63408345 None:intergenic 35.0%
TGCATTGCATATCTATGTGT+AGG + chr1_3:63408639-63408658 Msa0132390:intron 35.0%
TTGCATATCTATGTGTAGGA+TGG + chr1_3:63408643-63408662 Msa0132390:intron 35.0%
CTATTCAGTTCCTTTGTGAA+TGG + chr1_3:63408765-63408784 Msa0132390:three_prime_UTR 35.0%
!! ATTGAAGGTCTTGTTGTGTT+TGG - chr1_3:63408801-63408820 None:intergenic 35.0%
ACAACAAGACCTTCAATTGA+AGG + chr1_3:63408804-63408823 Msa0132390:three_prime_UTR 35.0%
TGCCAACTGTATATAACATG+TGG + chr1_3:63408853-63408872 Msa0132390:three_prime_UTR 35.0%
TTCCCAACCTTAGCAATGAA+GGG - chr1_3:63407517-63407536 None:intergenic 40.0%
!!! GATTTTGGCTAACAGGCAAT+CGG + chr1_3:63407932-63407951 Msa0132390:CDS 40.0%
! TTCAGACTCTGAAGACTGAA+GGG + chr1_3:63408009-63408028 Msa0132390:CDS 40.0%
GGACTGTAATCTTGAACTTC+AGG - chr1_3:63408287-63408306 None:intergenic 40.0%
! GACAAAAACCACTGCTGATT+TGG - chr1_3:63408439-63408458 None:intergenic 40.0%
CCACGAATCGCAGAAAATAA+TGG - chr1_3:63408585-63408604 None:intergenic 40.0%
! CCATTATTTTCTGCGATTCG+TGG + chr1_3:63408582-63408601 Msa0132390:intron 40.0%
CATATCTATGTGTAGGATGG+AGG + chr1_3:63408646-63408665 Msa0132390:intron 40.0%
AGGTTTGGCAACTTTGAGTA+TGG + chr1_3:63408666-63408685 Msa0132390:three_prime_UTR 40.0%
! AATAGATGCAGTGCTCTAGA+AGG - chr1_3:63408750-63408769 None:intergenic 40.0%
TCAGTTCCTTTGTGAATGGT+AGG + chr1_3:63408769-63408788 Msa0132390:three_prime_UTR 40.0%
CAATCTCCTACCATTCACAA+AGG - chr1_3:63408778-63408797 None:intergenic 40.0%
CAAGACCTTCAATTGAAGGA+TGG + chr1_3:63408808-63408827 Msa0132390:three_prime_UTR 40.0%
GGACCTTAGCAAACTGTATT+TGG + chr1_3:63408980-63408999 Msa0132390:three_prime_UTR 40.0%
!! GTTCTGAAATGCCTTCAGTT+TGG + chr1_3:63409097-63409116 Msa0132390:three_prime_UTR 40.0%
CGAAAACTGATCCAAACTGA+AGG - chr1_3:63409111-63409130 None:intergenic 40.0%
TTCCCAACCTTAGCAATGAA+GGG - chr1_3:63407517-63407536 None:intergenic 40.0%
!!! GATTTTGGCTAACAGGCAAT+CGG + chr1_3:63407932-63407951 Msa0132390:CDS 40.0%
! TTCAGACTCTGAAGACTGAA+GGG + chr1_3:63408009-63408028 Msa0132390:CDS 40.0%
GGACTGTAATCTTGAACTTC+AGG - chr1_3:63408287-63408306 None:intergenic 40.0%
! GACAAAAACCACTGCTGATT+TGG - chr1_3:63408439-63408458 None:intergenic 40.0%
CCACGAATCGCAGAAAATAA+TGG - chr1_3:63408585-63408604 None:intergenic 40.0%
! CCATTATTTTCTGCGATTCG+TGG + chr1_3:63408582-63408601 Msa0132390:intron 40.0%
CATATCTATGTGTAGGATGG+AGG + chr1_3:63408646-63408665 Msa0132390:intron 40.0%
AGGTTTGGCAACTTTGAGTA+TGG + chr1_3:63408666-63408685 Msa0132390:three_prime_UTR 40.0%
! AATAGATGCAGTGCTCTAGA+AGG - chr1_3:63408750-63408769 None:intergenic 40.0%
TCAGTTCCTTTGTGAATGGT+AGG + chr1_3:63408769-63408788 Msa0132390:three_prime_UTR 40.0%
CAATCTCCTACCATTCACAA+AGG - chr1_3:63408778-63408797 None:intergenic 40.0%
CAAGACCTTCAATTGAAGGA+TGG + chr1_3:63408808-63408827 Msa0132390:three_prime_UTR 40.0%
GGACCTTAGCAAACTGTATT+TGG + chr1_3:63408980-63408999 Msa0132390:three_prime_UTR 40.0%
!! GTTCTGAAATGCCTTCAGTT+TGG + chr1_3:63409097-63409116 Msa0132390:three_prime_UTR 40.0%
CGAAAACTGATCCAAACTGA+AGG - chr1_3:63409111-63409130 None:intergenic 40.0%
GCTCATTTACCTCTCACTGT+TGG - chr1_3:63407319-63407338 None:intergenic 45.0%
TAAGGCTCCCTTCATTGCTA+AGG + chr1_3:63407507-63407526 Msa0132390:intron 45.0%
CTCCCTTCATTGCTAAGGTT+GGG + chr1_3:63407512-63407531 Msa0132390:intron 45.0%
CTTCCCAACCTTAGCAATGA+AGG - chr1_3:63407518-63407537 None:intergenic 45.0%
TGCTAAGGTTGGGAAGTTGA+AGG + chr1_3:63407522-63407541 Msa0132390:intron 45.0%
TAAGGTTGGGAAGTTGAAGG+AGG + chr1_3:63407525-63407544 Msa0132390:intron 45.0%
TTGGACTGAGAAGACTGCTT+CGG + chr1_3:63407687-63407706 Msa0132390:intron 45.0%
TTCTTATGGCTGATCCGAAG+CGG + chr1_3:63407813-63407832 Msa0132390:CDS 45.0%
! GTTCAGACTCTGAAGACTGA+AGG + chr1_3:63408008-63408027 Msa0132390:CDS 45.0%
ATTGCTGAAGCAAACTCAGC+AGG - chr1_3:63408070-63408089 None:intergenic 45.0%
AACACTGCTTTCTCGGTTCT+AGG - chr1_3:63408108-63408127 None:intergenic 45.0%
TAGAACCGAGAAAGCAGTGT+TGG + chr1_3:63408107-63408126 Msa0132390:exon 45.0%
TGCTACCAACACTGCTTTCT+CGG - chr1_3:63408115-63408134 None:intergenic 45.0%
GCAACGACACAACGAATTCT+TGG - chr1_3:63408227-63408246 None:intergenic 45.0%
! GAATTCGTTGTGTCGTTGCT+AGG + chr1_3:63408228-63408247 Msa0132390:three_prime_UTR 45.0%
TTACAGTCCCATATGGAGCT+TGG + chr1_3:63408298-63408317 Msa0132390:three_prime_UTR 45.0%
TGTGGAACCAAGCTCCATAT+GGG - chr1_3:63408308-63408327 None:intergenic 45.0%
TTGTGGAACCAAGCTCCATA+TGG - chr1_3:63408309-63408328 None:intergenic 45.0%
TATGGAGCTTGGTTCCACAA+AGG + chr1_3:63408309-63408328 Msa0132390:three_prime_UTR 45.0%
!! CCACTGCTGATTTGGAAAAC+TGG - chr1_3:63408431-63408450 None:intergenic 45.0%
! CCAGTTTTCCAAATCAGCAG+TGG + chr1_3:63408428-63408447 Msa0132390:intron 45.0%
CTATGTGTAGGATGGAGGTT+TGG + chr1_3:63408651-63408670 Msa0132390:intron 45.0%
TGTTAACTTTCGCCACCGTA+CGG + chr1_3:63408694-63408713 Msa0132390:three_prime_UTR 45.0%
!! GCTCTAGAAGGTTGTGCATA+GGG - chr1_3:63408738-63408757 None:intergenic 45.0%
!! TGCTCTAGAAGGTTGTGCAT+AGG - chr1_3:63408739-63408758 None:intergenic 45.0%
GCTCATTTACCTCTCACTGT+TGG - chr1_3:63407319-63407338 None:intergenic 45.0%
TAAGGCTCCCTTCATTGCTA+AGG + chr1_3:63407507-63407526 Msa0132390:intron 45.0%
CTCCCTTCATTGCTAAGGTT+GGG + chr1_3:63407512-63407531 Msa0132390:intron 45.0%
CTTCCCAACCTTAGCAATGA+AGG - chr1_3:63407518-63407537 None:intergenic 45.0%
TGCTAAGGTTGGGAAGTTGA+AGG + chr1_3:63407522-63407541 Msa0132390:intron 45.0%
TAAGGTTGGGAAGTTGAAGG+AGG + chr1_3:63407525-63407544 Msa0132390:intron 45.0%
TTGGACTGAGAAGACTGCTT+CGG + chr1_3:63407687-63407706 Msa0132390:intron 45.0%
TTCTTATGGCTGATCCGAAG+CGG + chr1_3:63407813-63407832 Msa0132390:CDS 45.0%
! GTTCAGACTCTGAAGACTGA+AGG + chr1_3:63408008-63408027 Msa0132390:CDS 45.0%
ATTGCTGAAGCAAACTCAGC+AGG - chr1_3:63408070-63408089 None:intergenic 45.0%
AACACTGCTTTCTCGGTTCT+AGG - chr1_3:63408108-63408127 None:intergenic 45.0%
TAGAACCGAGAAAGCAGTGT+TGG + chr1_3:63408107-63408126 Msa0132390:exon 45.0%
TGCTACCAACACTGCTTTCT+CGG - chr1_3:63408115-63408134 None:intergenic 45.0%
GCAACGACACAACGAATTCT+TGG - chr1_3:63408227-63408246 None:intergenic 45.0%
! GAATTCGTTGTGTCGTTGCT+AGG + chr1_3:63408228-63408247 Msa0132390:three_prime_UTR 45.0%
TTACAGTCCCATATGGAGCT+TGG + chr1_3:63408298-63408317 Msa0132390:three_prime_UTR 45.0%
TGTGGAACCAAGCTCCATAT+GGG - chr1_3:63408308-63408327 None:intergenic 45.0%
TTGTGGAACCAAGCTCCATA+TGG - chr1_3:63408309-63408328 None:intergenic 45.0%
TATGGAGCTTGGTTCCACAA+AGG + chr1_3:63408309-63408328 Msa0132390:three_prime_UTR 45.0%
!! CCACTGCTGATTTGGAAAAC+TGG - chr1_3:63408431-63408450 None:intergenic 45.0%
! CCAGTTTTCCAAATCAGCAG+TGG + chr1_3:63408428-63408447 Msa0132390:intron 45.0%
CTATGTGTAGGATGGAGGTT+TGG + chr1_3:63408651-63408670 Msa0132390:intron 45.0%
TGTTAACTTTCGCCACCGTA+CGG + chr1_3:63408694-63408713 Msa0132390:three_prime_UTR 45.0%
!! GCTCTAGAAGGTTGTGCATA+GGG - chr1_3:63408738-63408757 None:intergenic 45.0%
!! TGCTCTAGAAGGTTGTGCAT+AGG - chr1_3:63408739-63408758 None:intergenic 45.0%
GGCTAGGTTCAAAGATGCCA+AGG - chr1_3:63407203-63407222 None:intergenic 50.0%
!! TTGGTGTGAGGGTGCTACAT+TGG - chr1_3:63407300-63407319 None:intergenic 50.0%
ACCTCTCACTGTTGGTGTGA+GGG - chr1_3:63407311-63407330 None:intergenic 50.0%
TACCTCTCACTGTTGGTGTG+AGG - chr1_3:63407312-63407331 None:intergenic 50.0%
GCTCCCTTCATTGCTAAGGT+TGG + chr1_3:63407511-63407530 Msa0132390:intron 50.0%
AAGACTGCTTCGGAGGAAGA+AGG + chr1_3:63407697-63407716 Msa0132390:intron 50.0%
TCTTATGGCTGATCCGAAGC+GGG + chr1_3:63407814-63407833 Msa0132390:CDS 50.0%
TGAAGCAAACTCAGCAGGAG+AGG - chr1_3:63408065-63408084 None:intergenic 50.0%
ATAGTGGCGCTATTGCGTAG+TGG + chr1_3:63408479-63408498 Msa0132390:intron 50.0%
CGATTCGTGGTTGACATCAC+TGG + chr1_3:63408595-63408614 Msa0132390:intron 50.0%
GTTAACTTTCGCCACCGTAC+GGG + chr1_3:63408695-63408714 Msa0132390:three_prime_UTR 50.0%
TTAACTTTCGCCACCGTACG+GGG + chr1_3:63408696-63408715 Msa0132390:three_prime_UTR 50.0%
GGCTAGGTTCAAAGATGCCA+AGG - chr1_3:63407203-63407222 None:intergenic 50.0%
!! TTGGTGTGAGGGTGCTACAT+TGG - chr1_3:63407300-63407319 None:intergenic 50.0%
ACCTCTCACTGTTGGTGTGA+GGG - chr1_3:63407311-63407330 None:intergenic 50.0%
TACCTCTCACTGTTGGTGTG+AGG - chr1_3:63407312-63407331 None:intergenic 50.0%
GCTCCCTTCATTGCTAAGGT+TGG + chr1_3:63407511-63407530 Msa0132390:intron 50.0%
AAGACTGCTTCGGAGGAAGA+AGG + chr1_3:63407697-63407716 Msa0132390:intron 50.0%
TCTTATGGCTGATCCGAAGC+GGG + chr1_3:63407814-63407833 Msa0132390:CDS 50.0%
TGAAGCAAACTCAGCAGGAG+AGG - chr1_3:63408065-63408084 None:intergenic 50.0%
ATAGTGGCGCTATTGCGTAG+TGG + chr1_3:63408479-63408498 Msa0132390:intron 50.0%
CGATTCGTGGTTGACATCAC+TGG + chr1_3:63408595-63408614 Msa0132390:intron 50.0%
GTTAACTTTCGCCACCGTAC+GGG + chr1_3:63408695-63408714 Msa0132390:three_prime_UTR 50.0%
TTAACTTTCGCCACCGTACG+GGG + chr1_3:63408696-63408715 Msa0132390:three_prime_UTR 50.0%
ACCCTCACACCAACAGTGAG+AGG + chr1_3:63407307-63407326 Msa0132390:intron 55.0%
GACTGAGAAGACTGCTTCGG+AGG + chr1_3:63407690-63407709 Msa0132390:intron 55.0%
AGCTTGGTTCCACAAAGGCC+AGG + chr1_3:63408314-63408333 Msa0132390:three_prime_UTR 55.0%
ACCCTCACACCAACAGTGAG+AGG + chr1_3:63407307-63407326 Msa0132390:intron 55.0%
GACTGAGAAGACTGCTTCGG+AGG + chr1_3:63407690-63407709 Msa0132390:intron 55.0%
AGCTTGGTTCCACAAAGGCC+AGG + chr1_3:63408314-63408333 Msa0132390:three_prime_UTR 55.0%
!! CTGATCCGAAGCGGGCTAAG+AGG + chr1_3:63407822-63407841 Msa0132390:CDS 60.0%
CAGCACCTCTTAGCCCGCTT+CGG - chr1_3:63407830-63407849 None:intergenic 60.0%
CATGTGAGCTGCTCCCCGTA+CGG - chr1_3:63408712-63408731 None:intergenic 60.0%
!! CTGATCCGAAGCGGGCTAAG+AGG + chr1_3:63407822-63407841 Msa0132390:CDS 60.0%
CAGCACCTCTTAGCCCGCTT+CGG - chr1_3:63407830-63407849 None:intergenic 60.0%
CATGTGAGCTGCTCCCCGTA+CGG - chr1_3:63408712-63408731 None:intergenic 60.0%
CGTACGGGGAGCAGCTCACA+TGG + chr1_3:63408710-63408729 Msa0132390:three_prime_UTR 65.0%
CGTACGGGGAGCAGCTCACA+TGG + chr1_3:63408710-63408729 Msa0132390:three_prime_UTR 65.0%
GTGAGCTGCTCCCCGTACGG+TGG - chr1_3:63408709-63408728 None:intergenic 70.0%
GTGAGCTGCTCCCCGTACGG+TGG - chr1_3:63408709-63408728 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 63406981 63409194 63406981 ID=Msa0132390;Name=Msa0132390
chr1_3 mRNA 63406981 63409194 63406981 ID=Msa0132390-mRNA-1;Parent=Msa0132390;Name=Msa0132390-mRNA-1;_AED=0.30;_eAED=0.31;_QI=0|0.75|0.6|1|0.75|0.8|5|671|91
chr1_3 exon 63406981 63406989 63406981 ID=Msa0132390-mRNA-1:exon:21252;Parent=Msa0132390-mRNA-1
chr1_3 exon 63407755 63407843 63407755 ID=Msa0132390-mRNA-1:exon:21253;Parent=Msa0132390-mRNA-1
chr1_3 exon 63407932 63408128 63407932 ID=Msa0132390-mRNA-1:exon:21254;Parent=Msa0132390-mRNA-1
chr1_3 exon 63408218 63408335 63408218 ID=Msa0132390-mRNA-1:exon:21255;Parent=Msa0132390-mRNA-1
chr1_3 exon 63408661 63409194 63408661 ID=Msa0132390-mRNA-1:exon:21256;Parent=Msa0132390-mRNA-1
chr1_3 CDS 63406981 63406989 63406981 ID=Msa0132390-mRNA-1:cds;Parent=Msa0132390-mRNA-1
chr1_3 CDS 63407755 63407843 63407755 ID=Msa0132390-mRNA-1:cds;Parent=Msa0132390-mRNA-1
chr1_3 CDS 63407932 63408109 63407932 ID=Msa0132390-mRNA-1:cds;Parent=Msa0132390-mRNA-1
chr1_3 three_prime_UTR 63408110 63408128 63408110 ID=Msa0132390-mRNA-1:three_prime_utr;Parent=Msa0132390-mRNA-1
chr1_3 three_prime_UTR 63408218 63408335 63408218 ID=Msa0132390-mRNA-1:three_prime_utr;Parent=Msa0132390-mRNA-1
chr1_3 three_prime_UTR 63408661 63409194 63408661 ID=Msa0132390-mRNA-1:three_prime_utr;Parent=Msa0132390-mRNA-1
Gene Sequence

>Msa0132390

ATGGGACAGACTGAGAAGAATGCTTCAGAGTTGAAGAAAATTATGGCTAATGAGAAAGTTGCTGAGATTCTTATGGCTGATCCGAAGCGGGCTAAGAGGATTTTGGCTAACAGGCAATCGGCTGCGCGTTCCATTGAGCTTAAGATGCGATATATTTTGGAGCTAGAACACAAGGTTCAGACTCTGAAGACTGAAGGGTTGTTTCTTCTCCAATTTCAGAATATGACACCTCTCCTGCTGAGTTTGCTTCAGCAATTGCTGAGTTATGAAGCCTAG
Protein sequence

>Msa0132390

MGQTEKNASELKKIMANEKVAEILMADPKRAKRILANRQSAARSIELKMRYILELEHKVQTLKTEGLFLLQFQNMTPLLLSLLQQLLSYEA*