AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1175620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1175620 MtrunA17_Chr4g0036071 76.812 69 16 0 1 69 334 402 5.76e-28 105
Msa1175620 MtrunA17_Chr2g0306581 81.250 48 9 0 15 62 1 48 1.75e-20 79.0
Msa1175620 MtrunA17_Chr1g0156051 69.492 59 18 0 1 59 361 419 8.07e-20 82.0
Msa1175620 MtrunA17_Chr2g0329551 61.765 68 26 0 2 69 196 263 1.74e-19 80.9
Msa1175620 MtrunA17_Chr5g0432701 66.667 60 20 0 10 69 142 201 1.31e-18 78.2
Msa1175620 MtrunA17_Chr3g0107491 66.667 60 20 0 10 69 160 219 1.35e-18 78.2
Msa1175620 MtrunA17_Chr6g0463571 70.909 55 16 0 11 65 135 189 3.04e-18 77.0
Msa1175620 MtrunA17_Chr3g0128781 60.000 60 24 0 10 69 91 150 4.20e-17 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1175620 AT4G38900.2 73.846 65 17 0 5 69 372 436 8.16e-24 94.0
Msa1175620 AT4G38900.3 73.846 65 17 0 5 69 372 436 8.16e-24 94.0
Msa1175620 AT2G21230.4 68.116 69 22 0 1 69 344 412 8.94e-24 93.6
Msa1175620 AT2G21230.1 68.116 69 22 0 1 69 344 412 8.94e-24 93.6
Msa1175620 AT2G21230.3 68.116 69 22 0 1 69 344 412 9.23e-24 93.6
Msa1175620 AT2G21230.2 69.118 68 21 0 2 69 345 412 1.04e-22 90.5
Msa1175620 AT4G38900.1 67.606 71 17 1 5 69 372 442 1.41e-21 87.4
Msa1175620 AT2G40620.1 70.000 60 18 0 10 69 131 190 1.97e-20 83.6
Msa1175620 AT2G31370.5 60.294 68 27 0 2 69 176 243 6.61e-19 79.7
Msa1175620 AT2G31370.2 60.294 68 27 0 2 69 176 243 6.91e-19 79.7
Msa1175620 AT2G31370.4 60.294 68 27 0 2 69 176 243 6.91e-19 79.7
Msa1175620 AT2G31370.1 60.294 68 27 0 2 69 176 243 6.91e-19 79.7
Msa1175620 AT2G31370.7 60.294 68 27 0 2 69 176 243 6.91e-19 79.7
Msa1175620 AT2G31370.3 60.294 68 27 0 2 69 176 243 6.91e-19 79.7
Msa1175620 AT2G31370.6 60.294 68 27 0 2 69 176 243 6.91e-19 79.7
Msa1175620 AT1G06850.2 65.000 60 21 0 10 69 131 190 5.58e-18 75.5
Msa1175620 AT1G06070.1 57.353 68 29 0 2 69 185 252 1.03e-17 76.6
Msa1175620 AT1G06850.1 65.000 60 21 0 10 69 131 190 1.32e-17 75.9
Msa1175620 AT1G43700.1 63.636 55 20 0 15 69 182 236 6.04e-16 71.2

Find 59 sgRNAs with CRISPR-Local

Find 276 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGGTTATTTCAGGATTT+TGG 0.101232 8_1:-7085812 Msa1175620:intron
GGACCTTAGCAAACTGTATT+TGG 0.168656 8_1:-7084749 Msa1175620:three_prime_UTR
GCTTAAGATGCGATATATTT+TGG 0.170519 8_1:-7085758 Msa1175620:CDS
ATTTGGAAAATAAATTTATA+AGG 0.205498 8_1:-7084732 Msa1175620:three_prime_UTR
AATAAATTTATAAGGGGAAT+TGG 0.239221 8_1:-7084724 Msa1175620:three_prime_UTR
TGCTACCAACACTGCTTTCT+CGG 0.291207 8_1:+7085617 None:intergenic
TTCAGAGTTGAAGAAAATTA+TGG 0.315927 8_1:-7085960 Msa1175620:CDS
TTTGGAAAATAAATTTATAA+GGG 0.316986 8_1:-7084731 Msa1175620:three_prime_UTR
AGGTTTGGCAACTTTGAGTA+TGG 0.321602 8_1:-7085063 Msa1175620:three_prime_UTR
GCAACGACACAACGAATTCT+TGG 0.332774 8_1:+7085505 None:intergenic
AACACTGCTTTCTCGGTTCT+AGG 0.357024 8_1:+7085624 None:intergenic
GGACTGTAATCTTGAACTTC+AGG 0.367870 8_1:+7085445 None:intergenic
GAGGTGTCATATTCTGAAAT+TGG 0.368658 8_1:+7085686 None:intergenic
ATAAATTTATAAGGGGAATT+GGG 0.372298 8_1:-7084723 Msa1175620:three_prime_UTR
GTTCTGAAATGCCTTCAGTT+TGG 0.388967 8_1:-7084632 Msa1175620:three_prime_UTR
AAAATAATACCTGGCCTTTG+TGG 0.394261 8_1:+7085406 None:intergenic
ATCTAATAATAATAATCAAC+TGG 0.395377 8_1:+7084834 None:intergenic
GAAAGTTGCTGAGATTCTTA+TGG 0.396164 8_1:-7085930 Msa1175620:CDS
ATTGAAGGTCTTGTTGTGTT+TGG 0.431019 8_1:+7084931 None:intergenic
GTTAACTTTCGCCACCGTAC+GGG 0.448129 8_1:-7085034 Msa1175620:three_prime_UTR
CAGCACCTCTTAGCCCGCTT+CGG 0.449837 8_1:+7085902 None:intergenic
CTATTCAGTTCCTTTGTGAA+TGG 0.454434 8_1:-7084964 Msa1175620:three_prime_UTR
TTGTGGAACCAAGCTCCATA+TGG 0.454668 8_1:+7085423 None:intergenic
TGTTAACTTTCGCCACCGTA+CGG 0.456246 8_1:-7085035 Msa1175620:three_prime_UTR
AATAGATGCAGTGCTCTAGA+AGG 0.460695 8_1:+7084982 None:intergenic
ATATCGCATCTTAAGCTCAA+TGG 0.469206 8_1:+7085765 None:intergenic
TTCAAGATTACAGTCCCATA+TGG 0.472759 8_1:-7085438 Msa1175620:three_prime_UTR
GAATTCGTTGTGTCGTTGCT+AGG 0.487493 8_1:-7085501 Msa1175620:three_prime_UTR
ACAACAAGACCTTCAATTGA+AGG 0.487972 8_1:-7084925 Msa1175620:three_prime_UTR
CTATGTGTAGGATGGAGGTT+TGG 0.488964 8_1:-7085078 Msa1175620:intron
GCTCTAGAAGGTTGTGCATA+GGG 0.490885 8_1:+7084994 None:intergenic
AAATTGTGTAAAGAACAATA+TGG 0.499131 8_1:+7084655 None:intergenic
TCAGTTCCTTTGTGAATGGT+AGG 0.502741 8_1:-7084960 Msa1175620:three_prime_UTR
TTCCACATGTTATATACAGT+TGG 0.506432 8_1:+7084874 None:intergenic
TTACAGTCCCATATGGAGCT+TGG 0.523649 8_1:-7085431 Msa1175620:three_prime_UTR
TTTCCAAATACAGTTTGCTA+AGG 0.524653 8_1:+7084746 None:intergenic
CTGATCCGAAGCGGGCTAAG+AGG 0.529672 8_1:-7085907 Msa1175620:intron
TGTGGAACCAAGCTCCATAT+GGG 0.530718 8_1:+7085424 None:intergenic
GTTCAGACTCTGAAGACTGA+AGG 0.542615 8_1:-7085721 Msa1175620:CDS
TGCTCTAGAAGGTTGTGCAT+AGG 0.543792 8_1:+7084993 None:intergenic
TTGGAAAATAAATTTATAAG+GGG 0.545493 8_1:-7084730 Msa1175620:three_prime_UTR
TCTTATGGCTGATCCGAAGC+GGG 0.549131 8_1:-7085915 Msa1175620:CDS
ATTGCTGAAGCAAACTCAGC+AGG 0.554785 8_1:+7085662 None:intergenic
CATATCTATGTGTAGGATGG+AGG 0.564335 8_1:-7085083 Msa1175620:intron
CATGTGAGCTGCTCCCCGTA+CGG 0.564411 8_1:+7085020 None:intergenic
TAGAACCGAGAAAGCAGTGT+TGG 0.572911 8_1:-7085622 Msa1175620:intron
AGCTTGGTTCCACAAAGGCC+AGG 0.580658 8_1:-7085415 Msa1175620:intron
TTCAGACTCTGAAGACTGAA+GGG 0.583079 8_1:-7085720 Msa1175620:CDS
TTAACTTTCGCCACCGTACG+GGG 0.586054 8_1:-7085033 Msa1175620:three_prime_UTR
CAAGACCTTCAATTGAAGGA+TGG 0.592333 8_1:-7084921 Msa1175620:three_prime_UTR
TGCCAACTGTATATAACATG+TGG 0.599624 8_1:-7084876 Msa1175620:three_prime_UTR
CGAAACATAACAGATGGGAC+AGG 0.602460 8_1:-7086761 None:intergenic
CGTACGGGGAGCAGCTCACA+TGG 0.603251 8_1:-7085019 Msa1175620:three_prime_UTR
TGAAGCAAACTCAGCAGGAG+AGG 0.607777 8_1:+7085667 None:intergenic
CGAAAACTGATCCAAACTGA+AGG 0.608245 8_1:+7084621 None:intergenic
TTCTTATGGCTGATCCGAAG+CGG 0.620106 8_1:-7085916 Msa1175620:CDS
GTGAGCTGCTCCCCGTACGG+TGG 0.624321 8_1:+7085023 None:intergenic
TATGGAGCTTGGTTCCACAA+AGG 0.680414 8_1:-7085420 Msa1175620:three_prime_UTR
CAATCTCCTACCATTCACAA+AGG 0.682616 8_1:+7084954 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAATCTACTTTTTTTT+TGG + chr8_1:7084800-7084819 None:intergenic 10.0%
!! TAAAAAAAAAAAATAATACC+TGG + chr8_1:7085911-7085930 None:intergenic 10.0%
!! ATTTGGAAAATAAATTTATA+AGG - chr8_1:7086573-7086592 Msa1175620:intron 10.0%
!! TTTGGAAAATAAATTTATAA+GGG - chr8_1:7086574-7086593 Msa1175620:intron 10.0%
!! ATCATAAAAAATAAAAAGAA+TGG + chr8_1:7086621-7086640 None:intergenic 10.0%
!!! AAAAAAATCTACTTTTTTTT+TGG + chr8_1:7084800-7084819 None:intergenic 10.0%
!! TAAAAAAAAAAAATAATACC+TGG + chr8_1:7085911-7085930 None:intergenic 10.0%
!! ATTTGGAAAATAAATTTATA+AGG - chr8_1:7086573-7086592 Msa1175620:intron 10.0%
!! TTTGGAAAATAAATTTATAA+GGG - chr8_1:7086574-7086593 Msa1175620:intron 10.0%
!! ATCATAAAAAATAAAAAGAA+TGG + chr8_1:7086621-7086640 None:intergenic 10.0%
!! AATAAAAAATCTCAAGAAAA+AGG + chr8_1:7084669-7084688 None:intergenic 15.0%
!!! TTTTCTTGAGATTTTTTATT+TGG - chr8_1:7084669-7084688 Msa1175620:three_prime_UTR 15.0%
!! TACCATTATAAATGATAATT+TGG - chr8_1:7084838-7084857 Msa1175620:three_prime_UTR 15.0%
!! TTCCAAATTATCATTTATAA+TGG + chr8_1:7084843-7084862 None:intergenic 15.0%
!! ATTATAAATGATAATTTGGA+AGG - chr8_1:7084842-7084861 Msa1175620:three_prime_UTR 15.0%
!!! AATTATGTTTTGACTAAATT+AGG - chr8_1:7084919-7084938 Msa1175620:three_prime_UTR 15.0%
!!! TCTTTGTTTTTAAATTGAAT+GGG - chr8_1:7084985-7085004 Msa1175620:three_prime_UTR 15.0%
!! AAAAACATTTAAACTTCATA+AGG + chr8_1:7085041-7085060 None:intergenic 15.0%
!!! ATTTAACTTGGATATTTTTT+TGG - chr8_1:7085475-7085494 Msa1175620:three_prime_UTR 15.0%
!!! TTTAACTTGGATATTTTTTT+GGG - chr8_1:7085476-7085495 Msa1175620:three_prime_UTR 15.0%
!! ATCTAATAATAATAATCAAC+TGG + chr8_1:7086474-7086493 None:intergenic 15.0%
!! TTGGAAAATAAATTTATAAG+GGG - chr8_1:7086575-7086594 Msa1175620:intron 15.0%
!! AATAAAAAATCTCAAGAAAA+AGG + chr8_1:7084669-7084688 None:intergenic 15.0%
!!! TTTTCTTGAGATTTTTTATT+TGG - chr8_1:7084669-7084688 Msa1175620:three_prime_UTR 15.0%
!! TACCATTATAAATGATAATT+TGG - chr8_1:7084838-7084857 Msa1175620:three_prime_UTR 15.0%
!! TTCCAAATTATCATTTATAA+TGG + chr8_1:7084843-7084862 None:intergenic 15.0%
!! ATTATAAATGATAATTTGGA+AGG - chr8_1:7084842-7084861 Msa1175620:three_prime_UTR 15.0%
!!! AATTATGTTTTGACTAAATT+AGG - chr8_1:7084919-7084938 Msa1175620:three_prime_UTR 15.0%
!!! TCTTTGTTTTTAAATTGAAT+GGG - chr8_1:7084985-7085004 Msa1175620:three_prime_UTR 15.0%
!! AAAAACATTTAAACTTCATA+AGG + chr8_1:7085041-7085060 None:intergenic 15.0%
!!! ATTTAACTTGGATATTTTTT+TGG - chr8_1:7085475-7085494 Msa1175620:three_prime_UTR 15.0%
!!! TTTAACTTGGATATTTTTTT+GGG - chr8_1:7085476-7085495 Msa1175620:three_prime_UTR 15.0%
!! ATCTAATAATAATAATCAAC+TGG + chr8_1:7086474-7086493 None:intergenic 15.0%
!! TTGGAAAATAAATTTATAAG+GGG - chr8_1:7086575-7086594 Msa1175620:intron 15.0%
!! AACAAAGACTACAAATTAAT+AGG + chr8_1:7084601-7084620 None:intergenic 20.0%
!!! GTCTTTGTTTTTAAATTGAA+TGG - chr8_1:7084984-7085003 Msa1175620:three_prime_UTR 20.0%
!!! TTGTGATTTATGTGTATTTT+AGG - chr8_1:7085059-7085078 Msa1175620:three_prime_UTR 20.0%
!!! TTTATGTGTATTTTAGGATA+AGG - chr8_1:7085065-7085084 Msa1175620:three_prime_UTR 20.0%
!! TTTAGTTAGTAATGTCAATA+TGG - chr8_1:7085445-7085464 Msa1175620:three_prime_UTR 20.0%
!!! ATATTTTTTTGGGTTATTTC+AGG - chr8_1:7085486-7085505 Msa1175620:three_prime_UTR 20.0%
!! AGAAATGATCAATAAAGATT+TGG + chr8_1:7085720-7085739 None:intergenic 20.0%
!! GTGTTAAATTATTATGATAG+CGG - chr8_1:7085774-7085793 Msa1175620:CDS 20.0%
!!! AAATTGTTAAAGAGTTTTGT+AGG - chr8_1:7086535-7086554 Msa1175620:intron 20.0%
!! AATAAATTTATAAGGGGAAT+TGG - chr8_1:7086581-7086600 Msa1175620:intron 20.0%
!! ATAAATTTATAAGGGGAATT+GGG - chr8_1:7086582-7086601 Msa1175620:intron 20.0%
!! AAATTGTGTAAAGAACAATA+TGG + chr8_1:7086653-7086672 None:intergenic 20.0%
!! AACAAAGACTACAAATTAAT+AGG + chr8_1:7084601-7084620 None:intergenic 20.0%
!!! GTCTTTGTTTTTAAATTGAA+TGG - chr8_1:7084984-7085003 Msa1175620:three_prime_UTR 20.0%
!!! TTGTGATTTATGTGTATTTT+AGG - chr8_1:7085059-7085078 Msa1175620:three_prime_UTR 20.0%
!!! TTTATGTGTATTTTAGGATA+AGG - chr8_1:7085065-7085084 Msa1175620:three_prime_UTR 20.0%
!! TTTAGTTAGTAATGTCAATA+TGG - chr8_1:7085445-7085464 Msa1175620:three_prime_UTR 20.0%
!!! ATATTTTTTTGGGTTATTTC+AGG - chr8_1:7085486-7085505 Msa1175620:three_prime_UTR 20.0%
!! AGAAATGATCAATAAAGATT+TGG + chr8_1:7085720-7085739 None:intergenic 20.0%
!! GTGTTAAATTATTATGATAG+CGG - chr8_1:7085774-7085793 Msa1175620:CDS 20.0%
!!! AAATTGTTAAAGAGTTTTGT+AGG - chr8_1:7086535-7086554 Msa1175620:intron 20.0%
!! AATAAATTTATAAGGGGAAT+TGG - chr8_1:7086581-7086600 Msa1175620:intron 20.0%
!! ATAAATTTATAAGGGGAATT+GGG - chr8_1:7086582-7086601 Msa1175620:intron 20.0%
!! AAATTGTGTAAAGAACAATA+TGG + chr8_1:7086653-7086672 None:intergenic 20.0%
! CTAAATTACAATCTGTCATT+TGG - chr8_1:7084634-7084653 Msa1175620:three_prime_UTR 25.0%
!!! GGTATTGTTCTTTTTAACTA+TGG - chr8_1:7084690-7084709 Msa1175620:three_prime_UTR 25.0%
!!! CATTTTTTTTGACTTCATGT+TGG - chr8_1:7084725-7084744 Msa1175620:three_prime_UTR 25.0%
!!! AATCTACTTTTTTTTTGGCT+AGG + chr8_1:7084795-7084814 None:intergenic 25.0%
! ATAAATGATAATTTGGAAGG+AGG - chr8_1:7084845-7084864 Msa1175620:three_prime_UTR 25.0%
! ATATGTATCTTGACTCTATT+TGG - chr8_1:7084962-7084981 Msa1175620:three_prime_UTR 25.0%
!!! TTTACTGCTCTTATTTTACT+TGG - chr8_1:7085198-7085217 Msa1175620:intron 25.0%
! CATCAAAATTAGTTCAAATC+AGG + chr8_1:7085241-7085260 None:intergenic 25.0%
!!! TTGAACTAATTTTGATGTCT+TGG - chr8_1:7085244-7085263 Msa1175620:intron 25.0%
! TTCAGAGTTGAAGAAAATTA+TGG - chr8_1:7085345-7085364 Msa1175620:intron 25.0%
! TATGGATGGTTAATTTAACT+TGG - chr8_1:7085463-7085482 Msa1175620:three_prime_UTR 25.0%
!! ATTAGCTTCTTAGTAATCAA+AGG + chr8_1:7085751-7085770 None:intergenic 25.0%
!!! ATTTTTCGAAATGCAGAAAA+TGG + chr8_1:7085943-7085962 None:intergenic 25.0%
! AAGTTCAGTATTCTATAGTA+TGG - chr8_1:7085963-7085982 Msa1175620:CDS 25.0%
!!! GTATGGTTATGCAATTTTTT+CGG - chr8_1:7085980-7085999 Msa1175620:intron 25.0%
! ATTGAAGGATGGAAAAAAAA+TGG - chr8_1:7086395-7086414 Msa1175620:intron 25.0%
! TTGAAGGATGGAAAAAAAAT+GGG - chr8_1:7086396-7086415 Msa1175620:intron 25.0%
!!! ATTTTAAGAATGCTGTTGAT+TGG + chr8_1:7086717-7086736 None:intergenic 25.0%
! CTAAATTACAATCTGTCATT+TGG - chr8_1:7084634-7084653 Msa1175620:three_prime_UTR 25.0%
!!! GGTATTGTTCTTTTTAACTA+TGG - chr8_1:7084690-7084709 Msa1175620:three_prime_UTR 25.0%
!!! CATTTTTTTTGACTTCATGT+TGG - chr8_1:7084725-7084744 Msa1175620:three_prime_UTR 25.0%
!!! AATCTACTTTTTTTTTGGCT+AGG + chr8_1:7084795-7084814 None:intergenic 25.0%
! ATAAATGATAATTTGGAAGG+AGG - chr8_1:7084845-7084864 Msa1175620:three_prime_UTR 25.0%
! ATATGTATCTTGACTCTATT+TGG - chr8_1:7084962-7084981 Msa1175620:three_prime_UTR 25.0%
!!! TTTACTGCTCTTATTTTACT+TGG - chr8_1:7085198-7085217 Msa1175620:intron 25.0%
! CATCAAAATTAGTTCAAATC+AGG + chr8_1:7085241-7085260 None:intergenic 25.0%
!!! TTGAACTAATTTTGATGTCT+TGG - chr8_1:7085244-7085263 Msa1175620:intron 25.0%
! TTCAGAGTTGAAGAAAATTA+TGG - chr8_1:7085345-7085364 Msa1175620:intron 25.0%
! TATGGATGGTTAATTTAACT+TGG - chr8_1:7085463-7085482 Msa1175620:three_prime_UTR 25.0%
!! ATTAGCTTCTTAGTAATCAA+AGG + chr8_1:7085751-7085770 None:intergenic 25.0%
!!! ATTTTTCGAAATGCAGAAAA+TGG + chr8_1:7085943-7085962 None:intergenic 25.0%
! AAGTTCAGTATTCTATAGTA+TGG - chr8_1:7085963-7085982 Msa1175620:CDS 25.0%
!!! GTATGGTTATGCAATTTTTT+CGG - chr8_1:7085980-7085999 Msa1175620:intron 25.0%
! ATTGAAGGATGGAAAAAAAA+TGG - chr8_1:7086395-7086414 Msa1175620:intron 25.0%
! TTGAAGGATGGAAAAAAAAT+GGG - chr8_1:7086396-7086415 Msa1175620:intron 25.0%
!!! ATTTTAAGAATGCTGTTGAT+TGG + chr8_1:7086717-7086736 None:intergenic 25.0%
TTGTAATTTAGATGAGGCAT+GGG + chr8_1:7084625-7084644 None:intergenic 30.0%
ATTGTAATTTAGATGAGGCA+TGG + chr8_1:7084626-7084645 None:intergenic 30.0%
GACAGATTGTAATTTAGATG+AGG + chr8_1:7084631-7084650 None:intergenic 30.0%
! GAGTCAAGATACATATTTTG+CGG + chr8_1:7084958-7084977 None:intergenic 30.0%
ACAATGTGTGCTTACAATAT+GGG - chr8_1:7085135-7085154 Msa1175620:intron 30.0%
GTTAGTAATGTCAATATGGA+TGG - chr8_1:7085449-7085468 Msa1175620:three_prime_UTR 30.0%
!! TTTGGGTTATTTCAGGATTT+TGG - chr8_1:7085493-7085512 Msa1175620:three_prime_UTR 30.0%
! GCTTAAGATGCGATATATTT+TGG - chr8_1:7085547-7085566 Msa1175620:intron 30.0%
TTGTCACTGTTGTCAAATAA+AGG - chr8_1:7086030-7086049 Msa1175620:intron 30.0%
TTGTCAAATAAAGGCTATAG+TGG - chr8_1:7086039-7086058 Msa1175620:intron 30.0%
! TTTTTCCATCCTTCAATTGA+AGG + chr8_1:7086392-7086411 None:intergenic 30.0%
TTCCACATGTTATATACAGT+TGG + chr8_1:7086434-7086453 None:intergenic 30.0%
!! TTTCCAAATACAGTTTGCTA+AGG + chr8_1:7086562-7086581 None:intergenic 30.0%
TTGTAATTTAGATGAGGCAT+GGG + chr8_1:7084625-7084644 None:intergenic 30.0%
ATTGTAATTTAGATGAGGCA+TGG + chr8_1:7084626-7084645 None:intergenic 30.0%
GACAGATTGTAATTTAGATG+AGG + chr8_1:7084631-7084650 None:intergenic 30.0%
! GAGTCAAGATACATATTTTG+CGG + chr8_1:7084958-7084977 None:intergenic 30.0%
ACAATGTGTGCTTACAATAT+GGG - chr8_1:7085135-7085154 Msa1175620:intron 30.0%
GTTAGTAATGTCAATATGGA+TGG - chr8_1:7085449-7085468 Msa1175620:three_prime_UTR 30.0%
!! TTTGGGTTATTTCAGGATTT+TGG - chr8_1:7085493-7085512 Msa1175620:three_prime_UTR 30.0%
! GCTTAAGATGCGATATATTT+TGG - chr8_1:7085547-7085566 Msa1175620:intron 30.0%
TTGTCACTGTTGTCAAATAA+AGG - chr8_1:7086030-7086049 Msa1175620:intron 30.0%
TTGTCAAATAAAGGCTATAG+TGG - chr8_1:7086039-7086058 Msa1175620:intron 30.0%
! TTTTTCCATCCTTCAATTGA+AGG + chr8_1:7086392-7086411 None:intergenic 30.0%
TTCCACATGTTATATACAGT+TGG + chr8_1:7086434-7086453 None:intergenic 30.0%
!! TTTCCAAATACAGTTTGCTA+AGG + chr8_1:7086562-7086581 None:intergenic 30.0%
TGTAATTTAGATGAGGCATG+GGG + chr8_1:7084624-7084643 None:intergenic 35.0%
ATGTCTAAGCTATAACTCCT+TGG - chr8_1:7084759-7084778 Msa1175620:three_prime_UTR 35.0%
!! GGGAAGTACTTTGAGATATT+TGG - chr8_1:7085005-7085024 Msa1175620:three_prime_UTR 35.0%
GACAATGTGTGCTTACAATA+TGG - chr8_1:7085134-7085153 Msa1175620:intron 35.0%
GAAAGTTGCTGAGATTCTTA+TGG - chr8_1:7085375-7085394 Msa1175620:intron 35.0%
!!! ATTTCAGGATTTTGGCTAAC+AGG - chr8_1:7085501-7085520 Msa1175620:three_prime_UTR 35.0%
ATATCGCATCTTAAGCTCAA+TGG + chr8_1:7085543-7085562 None:intergenic 35.0%
!!! TATTTTGGAGCTAGAACACA+AGG - chr8_1:7085562-7085581 Msa1175620:intron 35.0%
!! GAGGTGTCATATTCTGAAAT+TGG + chr8_1:7085622-7085641 None:intergenic 35.0%
TTCAAGATTACAGTCCCATA+TGG - chr8_1:7085867-7085886 Msa1175620:intron 35.0%
! AAAATAATACCTGGCCTTTG+TGG + chr8_1:7085902-7085921 None:intergenic 35.0%
TGCATTGCATATCTATGTGT+AGG - chr8_1:7086215-7086234 Msa1175620:intron 35.0%
TTGCATATCTATGTGTAGGA+TGG - chr8_1:7086219-7086238 Msa1175620:intron 35.0%
CTATTCAGTTCCTTTGTGAA+TGG - chr8_1:7086341-7086360 Msa1175620:intron 35.0%
!! ATTGAAGGTCTTGTTGTGTT+TGG + chr8_1:7086377-7086396 None:intergenic 35.0%
ACAACAAGACCTTCAATTGA+AGG - chr8_1:7086380-7086399 Msa1175620:intron 35.0%
TGCCAACTGTATATAACATG+TGG - chr8_1:7086429-7086448 Msa1175620:intron 35.0%
TGTAATTTAGATGAGGCATG+GGG + chr8_1:7084624-7084643 None:intergenic 35.0%
ATGTCTAAGCTATAACTCCT+TGG - chr8_1:7084759-7084778 Msa1175620:three_prime_UTR 35.0%
!! GGGAAGTACTTTGAGATATT+TGG - chr8_1:7085005-7085024 Msa1175620:three_prime_UTR 35.0%
GACAATGTGTGCTTACAATA+TGG - chr8_1:7085134-7085153 Msa1175620:intron 35.0%
GAAAGTTGCTGAGATTCTTA+TGG - chr8_1:7085375-7085394 Msa1175620:intron 35.0%
!!! ATTTCAGGATTTTGGCTAAC+AGG - chr8_1:7085501-7085520 Msa1175620:three_prime_UTR 35.0%
ATATCGCATCTTAAGCTCAA+TGG + chr8_1:7085543-7085562 None:intergenic 35.0%
!!! TATTTTGGAGCTAGAACACA+AGG - chr8_1:7085562-7085581 Msa1175620:intron 35.0%
!! GAGGTGTCATATTCTGAAAT+TGG + chr8_1:7085622-7085641 None:intergenic 35.0%
TTCAAGATTACAGTCCCATA+TGG - chr8_1:7085867-7085886 Msa1175620:intron 35.0%
! AAAATAATACCTGGCCTTTG+TGG + chr8_1:7085902-7085921 None:intergenic 35.0%
TGCATTGCATATCTATGTGT+AGG - chr8_1:7086215-7086234 Msa1175620:intron 35.0%
TTGCATATCTATGTGTAGGA+TGG - chr8_1:7086219-7086238 Msa1175620:intron 35.0%
CTATTCAGTTCCTTTGTGAA+TGG - chr8_1:7086341-7086360 Msa1175620:intron 35.0%
!! ATTGAAGGTCTTGTTGTGTT+TGG + chr8_1:7086377-7086396 None:intergenic 35.0%
ACAACAAGACCTTCAATTGA+AGG - chr8_1:7086380-7086399 Msa1175620:intron 35.0%
TGCCAACTGTATATAACATG+TGG - chr8_1:7086429-7086448 Msa1175620:intron 35.0%
TTCCCAACCTTAGCAATGAA+GGG + chr8_1:7085093-7085112 None:intergenic 40.0%
!!! GATTTTGGCTAACAGGCAAT+CGG - chr8_1:7085508-7085527 Msa1175620:three_prime_UTR 40.0%
! TTCAGACTCTGAAGACTGAA+GGG - chr8_1:7085585-7085604 Msa1175620:intron 40.0%
GGACTGTAATCTTGAACTTC+AGG + chr8_1:7085863-7085882 None:intergenic 40.0%
! GACAAAAACCACTGCTGATT+TGG + chr8_1:7086015-7086034 None:intergenic 40.0%
CCACGAATCGCAGAAAATAA+TGG + chr8_1:7086161-7086180 None:intergenic 40.0%
! CCATTATTTTCTGCGATTCG+TGG - chr8_1:7086158-7086177 Msa1175620:intron 40.0%
CATATCTATGTGTAGGATGG+AGG - chr8_1:7086222-7086241 Msa1175620:intron 40.0%
AGGTTTGGCAACTTTGAGTA+TGG - chr8_1:7086242-7086261 Msa1175620:intron 40.0%
! AATAGATGCAGTGCTCTAGA+AGG + chr8_1:7086326-7086345 None:intergenic 40.0%
TCAGTTCCTTTGTGAATGGT+AGG - chr8_1:7086345-7086364 Msa1175620:intron 40.0%
CAATCTCCTACCATTCACAA+AGG + chr8_1:7086354-7086373 None:intergenic 40.0%
CAAGACCTTCAATTGAAGGA+TGG - chr8_1:7086384-7086403 Msa1175620:intron 40.0%
GGACCTTAGCAAACTGTATT+TGG - chr8_1:7086556-7086575 Msa1175620:intron 40.0%
!! GTTCTGAAATGCCTTCAGTT+TGG - chr8_1:7086673-7086692 Msa1175620:intron 40.0%
CGAAAACTGATCCAAACTGA+AGG + chr8_1:7086687-7086706 None:intergenic 40.0%
TTCCCAACCTTAGCAATGAA+GGG + chr8_1:7085093-7085112 None:intergenic 40.0%
!!! GATTTTGGCTAACAGGCAAT+CGG - chr8_1:7085508-7085527 Msa1175620:three_prime_UTR 40.0%
! TTCAGACTCTGAAGACTGAA+GGG - chr8_1:7085585-7085604 Msa1175620:intron 40.0%
GGACTGTAATCTTGAACTTC+AGG + chr8_1:7085863-7085882 None:intergenic 40.0%
! GACAAAAACCACTGCTGATT+TGG + chr8_1:7086015-7086034 None:intergenic 40.0%
CCACGAATCGCAGAAAATAA+TGG + chr8_1:7086161-7086180 None:intergenic 40.0%
! CCATTATTTTCTGCGATTCG+TGG - chr8_1:7086158-7086177 Msa1175620:intron 40.0%
CATATCTATGTGTAGGATGG+AGG - chr8_1:7086222-7086241 Msa1175620:intron 40.0%
AGGTTTGGCAACTTTGAGTA+TGG - chr8_1:7086242-7086261 Msa1175620:intron 40.0%
! AATAGATGCAGTGCTCTAGA+AGG + chr8_1:7086326-7086345 None:intergenic 40.0%
TCAGTTCCTTTGTGAATGGT+AGG - chr8_1:7086345-7086364 Msa1175620:intron 40.0%
CAATCTCCTACCATTCACAA+AGG + chr8_1:7086354-7086373 None:intergenic 40.0%
CAAGACCTTCAATTGAAGGA+TGG - chr8_1:7086384-7086403 Msa1175620:intron 40.0%
GGACCTTAGCAAACTGTATT+TGG - chr8_1:7086556-7086575 Msa1175620:intron 40.0%
!! GTTCTGAAATGCCTTCAGTT+TGG - chr8_1:7086673-7086692 Msa1175620:intron 40.0%
CGAAAACTGATCCAAACTGA+AGG + chr8_1:7086687-7086706 None:intergenic 40.0%
GCTCATTTACCTCTCACTGT+TGG + chr8_1:7084895-7084914 None:intergenic 45.0%
TAAGGCTCCCTTCATTGCTA+AGG - chr8_1:7085083-7085102 Msa1175620:intron 45.0%
CTCCCTTCATTGCTAAGGTT+GGG - chr8_1:7085088-7085107 Msa1175620:intron 45.0%
CTTCCCAACCTTAGCAATGA+AGG + chr8_1:7085094-7085113 None:intergenic 45.0%
TGCTAAGGTTGGGAAGTTGA+AGG - chr8_1:7085098-7085117 Msa1175620:intron 45.0%
TAAGGTTGGGAAGTTGAAGG+AGG - chr8_1:7085101-7085120 Msa1175620:intron 45.0%
TTGGACTGAGAAGACTGCTT+CGG - chr8_1:7085263-7085282 Msa1175620:intron 45.0%
TTCTTATGGCTGATCCGAAG+CGG - chr8_1:7085389-7085408 Msa1175620:intron 45.0%
! GTTCAGACTCTGAAGACTGA+AGG - chr8_1:7085584-7085603 Msa1175620:intron 45.0%
ATTGCTGAAGCAAACTCAGC+AGG + chr8_1:7085646-7085665 None:intergenic 45.0%
AACACTGCTTTCTCGGTTCT+AGG + chr8_1:7085684-7085703 None:intergenic 45.0%
TAGAACCGAGAAAGCAGTGT+TGG - chr8_1:7085683-7085702 Msa1175620:CDS 45.0%
TGCTACCAACACTGCTTTCT+CGG + chr8_1:7085691-7085710 None:intergenic 45.0%
GCAACGACACAACGAATTCT+TGG + chr8_1:7085803-7085822 None:intergenic 45.0%
! GAATTCGTTGTGTCGTTGCT+AGG - chr8_1:7085804-7085823 Msa1175620:intron 45.0%
TTACAGTCCCATATGGAGCT+TGG - chr8_1:7085874-7085893 Msa1175620:intron 45.0%
TGTGGAACCAAGCTCCATAT+GGG + chr8_1:7085884-7085903 None:intergenic 45.0%
TTGTGGAACCAAGCTCCATA+TGG + chr8_1:7085885-7085904 None:intergenic 45.0%
TATGGAGCTTGGTTCCACAA+AGG - chr8_1:7085885-7085904 Msa1175620:intron 45.0%
!! CCACTGCTGATTTGGAAAAC+TGG + chr8_1:7086007-7086026 None:intergenic 45.0%
! CCAGTTTTCCAAATCAGCAG+TGG - chr8_1:7086004-7086023 Msa1175620:intron 45.0%
CTATGTGTAGGATGGAGGTT+TGG - chr8_1:7086227-7086246 Msa1175620:intron 45.0%
TGTTAACTTTCGCCACCGTA+CGG - chr8_1:7086270-7086289 Msa1175620:intron 45.0%
!! GCTCTAGAAGGTTGTGCATA+GGG + chr8_1:7086314-7086333 None:intergenic 45.0%
!! TGCTCTAGAAGGTTGTGCAT+AGG + chr8_1:7086315-7086334 None:intergenic 45.0%
GCTCATTTACCTCTCACTGT+TGG + chr8_1:7084895-7084914 None:intergenic 45.0%
TAAGGCTCCCTTCATTGCTA+AGG - chr8_1:7085083-7085102 Msa1175620:intron 45.0%
CTCCCTTCATTGCTAAGGTT+GGG - chr8_1:7085088-7085107 Msa1175620:intron 45.0%
CTTCCCAACCTTAGCAATGA+AGG + chr8_1:7085094-7085113 None:intergenic 45.0%
TGCTAAGGTTGGGAAGTTGA+AGG - chr8_1:7085098-7085117 Msa1175620:intron 45.0%
TAAGGTTGGGAAGTTGAAGG+AGG - chr8_1:7085101-7085120 Msa1175620:intron 45.0%
TTGGACTGAGAAGACTGCTT+CGG - chr8_1:7085263-7085282 Msa1175620:intron 45.0%
TTCTTATGGCTGATCCGAAG+CGG - chr8_1:7085389-7085408 Msa1175620:intron 45.0%
! GTTCAGACTCTGAAGACTGA+AGG - chr8_1:7085584-7085603 Msa1175620:intron 45.0%
ATTGCTGAAGCAAACTCAGC+AGG + chr8_1:7085646-7085665 None:intergenic 45.0%
AACACTGCTTTCTCGGTTCT+AGG + chr8_1:7085684-7085703 None:intergenic 45.0%
TAGAACCGAGAAAGCAGTGT+TGG - chr8_1:7085683-7085702 Msa1175620:CDS 45.0%
TGCTACCAACACTGCTTTCT+CGG + chr8_1:7085691-7085710 None:intergenic 45.0%
GCAACGACACAACGAATTCT+TGG + chr8_1:7085803-7085822 None:intergenic 45.0%
! GAATTCGTTGTGTCGTTGCT+AGG - chr8_1:7085804-7085823 Msa1175620:intron 45.0%
TTACAGTCCCATATGGAGCT+TGG - chr8_1:7085874-7085893 Msa1175620:intron 45.0%
TGTGGAACCAAGCTCCATAT+GGG + chr8_1:7085884-7085903 None:intergenic 45.0%
TTGTGGAACCAAGCTCCATA+TGG + chr8_1:7085885-7085904 None:intergenic 45.0%
TATGGAGCTTGGTTCCACAA+AGG - chr8_1:7085885-7085904 Msa1175620:intron 45.0%
!! CCACTGCTGATTTGGAAAAC+TGG + chr8_1:7086007-7086026 None:intergenic 45.0%
! CCAGTTTTCCAAATCAGCAG+TGG - chr8_1:7086004-7086023 Msa1175620:intron 45.0%
CTATGTGTAGGATGGAGGTT+TGG - chr8_1:7086227-7086246 Msa1175620:intron 45.0%
TGTTAACTTTCGCCACCGTA+CGG - chr8_1:7086270-7086289 Msa1175620:intron 45.0%
!! GCTCTAGAAGGTTGTGCATA+GGG + chr8_1:7086314-7086333 None:intergenic 45.0%
!! TGCTCTAGAAGGTTGTGCAT+AGG + chr8_1:7086315-7086334 None:intergenic 45.0%
GGCTAGGTTCAAAGATGCCA+AGG + chr8_1:7084779-7084798 None:intergenic 50.0%
!! TTGGTGTGAGGGTGCTACAT+TGG + chr8_1:7084876-7084895 None:intergenic 50.0%
ACCTCTCACTGTTGGTGTGA+GGG + chr8_1:7084887-7084906 None:intergenic 50.0%
TACCTCTCACTGTTGGTGTG+AGG + chr8_1:7084888-7084907 None:intergenic 50.0%
GCTCCCTTCATTGCTAAGGT+TGG - chr8_1:7085087-7085106 Msa1175620:intron 50.0%
AAGACTGCTTCGGAGGAAGA+AGG - chr8_1:7085273-7085292 Msa1175620:intron 50.0%
TCTTATGGCTGATCCGAAGC+GGG - chr8_1:7085390-7085409 Msa1175620:intron 50.0%
TGAAGCAAACTCAGCAGGAG+AGG + chr8_1:7085641-7085660 None:intergenic 50.0%
ATAGTGGCGCTATTGCGTAG+TGG - chr8_1:7086055-7086074 Msa1175620:intron 50.0%
CGATTCGTGGTTGACATCAC+TGG - chr8_1:7086171-7086190 Msa1175620:intron 50.0%
GTTAACTTTCGCCACCGTAC+GGG - chr8_1:7086271-7086290 Msa1175620:intron 50.0%
TTAACTTTCGCCACCGTACG+GGG - chr8_1:7086272-7086291 Msa1175620:intron 50.0%
GGCTAGGTTCAAAGATGCCA+AGG + chr8_1:7084779-7084798 None:intergenic 50.0%
!! TTGGTGTGAGGGTGCTACAT+TGG + chr8_1:7084876-7084895 None:intergenic 50.0%
ACCTCTCACTGTTGGTGTGA+GGG + chr8_1:7084887-7084906 None:intergenic 50.0%
TACCTCTCACTGTTGGTGTG+AGG + chr8_1:7084888-7084907 None:intergenic 50.0%
GCTCCCTTCATTGCTAAGGT+TGG - chr8_1:7085087-7085106 Msa1175620:intron 50.0%
AAGACTGCTTCGGAGGAAGA+AGG - chr8_1:7085273-7085292 Msa1175620:intron 50.0%
TCTTATGGCTGATCCGAAGC+GGG - chr8_1:7085390-7085409 Msa1175620:intron 50.0%
TGAAGCAAACTCAGCAGGAG+AGG + chr8_1:7085641-7085660 None:intergenic 50.0%
ATAGTGGCGCTATTGCGTAG+TGG - chr8_1:7086055-7086074 Msa1175620:intron 50.0%
CGATTCGTGGTTGACATCAC+TGG - chr8_1:7086171-7086190 Msa1175620:intron 50.0%
GTTAACTTTCGCCACCGTAC+GGG - chr8_1:7086271-7086290 Msa1175620:intron 50.0%
TTAACTTTCGCCACCGTACG+GGG - chr8_1:7086272-7086291 Msa1175620:intron 50.0%
ACCCTCACACCAACAGTGAG+AGG - chr8_1:7084883-7084902 Msa1175620:three_prime_UTR 55.0%
GACTGAGAAGACTGCTTCGG+AGG - chr8_1:7085266-7085285 Msa1175620:intron 55.0%
AGCTTGGTTCCACAAAGGCC+AGG - chr8_1:7085890-7085909 Msa1175620:intron 55.0%
ACCCTCACACCAACAGTGAG+AGG - chr8_1:7084883-7084902 Msa1175620:three_prime_UTR 55.0%
GACTGAGAAGACTGCTTCGG+AGG - chr8_1:7085266-7085285 Msa1175620:intron 55.0%
AGCTTGGTTCCACAAAGGCC+AGG - chr8_1:7085890-7085909 Msa1175620:intron 55.0%
!! CTGATCCGAAGCGGGCTAAG+AGG - chr8_1:7085398-7085417 Msa1175620:intron 60.0%
CAGCACCTCTTAGCCCGCTT+CGG + chr8_1:7085406-7085425 None:intergenic 60.0%
CATGTGAGCTGCTCCCCGTA+CGG + chr8_1:7086288-7086307 None:intergenic 60.0%
!! CTGATCCGAAGCGGGCTAAG+AGG - chr8_1:7085398-7085417 Msa1175620:intron 60.0%
CAGCACCTCTTAGCCCGCTT+CGG + chr8_1:7085406-7085425 None:intergenic 60.0%
CATGTGAGCTGCTCCCCGTA+CGG + chr8_1:7086288-7086307 None:intergenic 60.0%
CGTACGGGGAGCAGCTCACA+TGG - chr8_1:7086286-7086305 Msa1175620:intron 65.0%
CGTACGGGGAGCAGCTCACA+TGG - chr8_1:7086286-7086305 Msa1175620:intron 65.0%
GTGAGCTGCTCCCCGTACGG+TGG + chr8_1:7086285-7086304 None:intergenic 70.0%
GTGAGCTGCTCCCCGTACGG+TGG + chr8_1:7086285-7086304 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 7084557 7086770 7084557 ID=Msa1175620;Name=Msa1175620
chr8_1 mRNA 7084557 7086770 7084557 ID=Msa1175620-mRNA-1;Parent=Msa1175620;Name=Msa1175620-mRNA-1;_AED=0.30;_eAED=0.31;_QI=0|0.75|0.6|1|0.75|0.8|5|671|91
chr8_1 exon 7084557 7085090 7084557 ID=Msa1175620-mRNA-1:exon:2572;Parent=Msa1175620-mRNA-1
chr8_1 exon 7085416 7085533 7085416 ID=Msa1175620-mRNA-1:exon:2571;Parent=Msa1175620-mRNA-1
chr8_1 exon 7085623 7085819 7085623 ID=Msa1175620-mRNA-1:exon:2570;Parent=Msa1175620-mRNA-1
chr8_1 exon 7085908 7085996 7085908 ID=Msa1175620-mRNA-1:exon:2569;Parent=Msa1175620-mRNA-1
chr8_1 exon 7086762 7086770 7086762 ID=Msa1175620-mRNA-1:exon:2568;Parent=Msa1175620-mRNA-1
chr8_1 CDS 7086762 7086770 7086762 ID=Msa1175620-mRNA-1:cds;Parent=Msa1175620-mRNA-1
chr8_1 CDS 7085908 7085996 7085908 ID=Msa1175620-mRNA-1:cds;Parent=Msa1175620-mRNA-1
chr8_1 CDS 7085642 7085819 7085642 ID=Msa1175620-mRNA-1:cds;Parent=Msa1175620-mRNA-1
chr8_1 three_prime_UTR 7085623 7085641 7085623 ID=Msa1175620-mRNA-1:three_prime_utr;Parent=Msa1175620-mRNA-1
chr8_1 three_prime_UTR 7085416 7085533 7085416 ID=Msa1175620-mRNA-1:three_prime_utr;Parent=Msa1175620-mRNA-1
chr8_1 three_prime_UTR 7084557 7085090 7084557 ID=Msa1175620-mRNA-1:three_prime_utr;Parent=Msa1175620-mRNA-1
Gene Sequence

>Msa1175620

ATGGGACAGACTGAGAAGAATGCTTCAGAGTTGAAGAAAATTATGGCTAATGAGAAAGTTGCTGAGATTCTTATGGCTGATCCGAAGCGGGCTAAGAGGATTTTGGCTAACAGGCAATCGGCTGCGCGTTCCATTGAGCTTAAGATGCGATATATTTTGGAGCTAGAACACAAGGTTCAGACTCTGAAGACTGAAGGGTTGTTTCTTCTCCAATTTCAGAATATGACACCTCTCCTGCTGAGTTTGCTTCAGCAATTGCTGAGTTATGAAGCCTAG
Protein sequence

>Msa1175620

MGQTEKNASELKKIMANEKVAEILMADPKRAKRILANRQSAARSIELKMRYILELEHKVQTLKTEGLFLLQFQNMTPLLLSLLQQLLSYEA*