Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0132610 | XP_003592099.1 | 95.794 | 214 | 9 | 0 | 1 | 214 | 1 | 214 | 7.53e-146 | 415 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0132610 | G7IBZ6 | 95.794 | 214 | 9 | 0 | 1 | 214 | 1 | 214 | 3.60e-146 | 415 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0132610 | Msa0577880 | 0.904632 | 1.002269e-79 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0132610 | MtrunA17_Chr1g0201801 | 95.794 | 214 | 9 | 0 | 1 | 214 | 1 | 214 | 6.92e-150 | 415 |
| Msa0132610 | MtrunA17_Chr6g0464341 | 56.566 | 99 | 37 | 3 | 50 | 145 | 36 | 131 | 7.74e-23 | 96.3 |
| Msa0132610 | MtrunA17_Chr6g0472121 | 59.184 | 98 | 34 | 3 | 50 | 144 | 9 | 103 | 9.14e-23 | 95.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0132610 | AT1G76770.1 | 55.118 | 127 | 54 | 1 | 1 | 124 | 1 | 127 | 2.18e-40 | 138 |
| Msa0132610 | AT1G76780.3 | 51.181 | 127 | 59 | 1 | 1 | 124 | 1 | 127 | 8.27e-35 | 130 |
| Msa0132610 | AT1G76780.2 | 51.181 | 127 | 59 | 1 | 1 | 124 | 1 | 127 | 8.27e-35 | 130 |
Find 35 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTGTCCACCTTTGGTTCT+TGG | 0.114619 | 1_3:-63825662 | Msa0132610:CDS |
| AATCCCTAATGGAGTTGTTT+TGG | 0.253932 | 1_3:-63825927 | Msa0132610:CDS |
| CTAGGACGTCTCTTCTTAAC+AGG | 0.266778 | 1_3:+63825698 | None:intergenic |
| TTATCCAAAACAACTCCATT+AGG | 0.283059 | 1_3:+63825923 | None:intergenic |
| AAGAAAGAGCTGAAAATAAA+AGG | 0.299279 | 1_3:-63825968 | Msa0132610:CDS |
| GACAGGTCCTGCTCTGTCTT+TGG | 0.343396 | 1_3:+63826486 | None:intergenic |
| TATCCAAAACAACTCCATTA+GGG | 0.355864 | 1_3:+63825924 | None:intergenic |
| ATTGCTATGGAGGTTGAGTT+AGG | 0.385137 | 1_3:-63826565 | None:intergenic |
| ATGGCAACCTTGTCCACCTT+TGG | 0.390972 | 1_3:-63825669 | Msa0132610:CDS |
| AAGAAGTTTAAAATCCCTAA+TGG | 0.416386 | 1_3:-63825938 | Msa0132610:CDS |
| TGTCCACCTTTGGTTCTTGG+AGG | 0.424444 | 1_3:-63825659 | Msa0132610:CDS |
| GGCAAACTGAAAATCAGAAA+TGG | 0.432210 | 1_3:+63826507 | None:intergenic |
| CAGTTTGCCAAAGACAGAGC+AGG | 0.497784 | 1_3:-63826493 | Msa0132610:CDS |
| AGAAGAAGTGAAGGAAGAAG+AGG | 0.509148 | 1_3:-63825807 | Msa0132610:CDS |
| GTTTCTTTAGATAAGAAGAC+AGG | 0.514312 | 1_3:+63826469 | None:intergenic |
| GACATCAACATTAACAAAGA+TGG | 0.531815 | 1_3:-63826049 | Msa0132610:CDS |
| CTGAAAATCAGAAATGGAAG+TGG | 0.544169 | 1_3:+63826513 | None:intergenic |
| GAAGAAGTGAAGGAAGAAGA+GGG | 0.550743 | 1_3:-63825806 | Msa0132610:CDS |
| CCAAGAACCAAAGGTGGACA+AGG | 0.550847 | 1_3:+63825662 | None:intergenic |
| TAGCAAAAGGAGTACTGAGT+GGG | 0.567184 | 1_3:-63825838 | Msa0132610:CDS |
| TACAGATGATAAGAGTGAAG+AGG | 0.572349 | 1_3:-63825729 | Msa0132610:CDS |
| AAAGGAGTACTGAGTGGGGT+GGG | 0.574936 | 1_3:-63825833 | Msa0132610:CDS |
| ACCTTTGAGATGTGCTGTGA+GGG | 0.582015 | 1_3:+63826432 | None:intergenic |
| GCTAAGTATTACGAAGAGGA+AGG | 0.582114 | 1_3:-63825893 | Msa0132610:CDS |
| ACCCTCACAGCACATCTCAA+AGG | 0.582279 | 1_3:-63826433 | Msa0132610:intron |
| AAAGGTGGACAAGGTTGCCA+TGG | 0.584568 | 1_3:+63825671 | None:intergenic |
| CAAAGCTAAGTATTACGAAG+AGG | 0.607521 | 1_3:-63825897 | Msa0132610:CDS |
| GGTGGGAATAGAAGAAGTGA+AGG | 0.616063 | 1_3:-63825816 | Msa0132610:CDS |
| AAAAGGAGTACTGAGTGGGG+TGG | 0.620358 | 1_3:-63825834 | Msa0132610:CDS |
| GTTGATCCTCCAAGAACCAA+AGG | 0.621557 | 1_3:+63825653 | None:intergenic |
| GATCCTCCAAGAACCAAAGG+TGG | 0.669806 | 1_3:+63825656 | None:intergenic |
| AGCAAAAGGAGTACTGAGTG+GGG | 0.702706 | 1_3:-63825837 | Msa0132610:CDS |
| AACCTTTGAGATGTGCTGTG+AGG | 0.714535 | 1_3:+63826431 | None:intergenic |
| AGAGACGTCCTAGAAAACCA+TGG | 0.719572 | 1_3:-63825688 | Msa0132610:CDS |
| ATAGCAAAAGGAGTACTGAG+TGG | 0.745850 | 1_3:-63825839 | Msa0132610:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CATCTTATGATATGTCTATA+AGG | - | chr1_3:63825855-63825874 | Msa0132610:CDS | 25.0% |
| ! | ATATGTTCATCATATCAACA+TGG | + | chr1_3:63826061-63826080 | None:intergenic | 25.0% |
| ! | AAGAAAGAGCTGAAAATAAA+AGG | - | chr1_3:63826186-63826205 | Msa0132610:intron | 25.0% |
| !! | AAGAAGTTTAAAATCCCTAA+TGG | - | chr1_3:63826216-63826235 | Msa0132610:intron | 25.0% |
| ! | CATCTTATGATATGTCTATA+AGG | - | chr1_3:63825855-63825874 | Msa0132610:CDS | 25.0% |
| ! | ATATGTTCATCATATCAACA+TGG | + | chr1_3:63826061-63826080 | None:intergenic | 25.0% |
| ! | AAGAAAGAGCTGAAAATAAA+AGG | - | chr1_3:63826186-63826205 | Msa0132610:intron | 25.0% |
| !! | AAGAAGTTTAAAATCCCTAA+TGG | - | chr1_3:63826216-63826235 | Msa0132610:intron | 25.0% |
| GTTTCTTTAGATAAGAAGAC+AGG | + | chr1_3:63825688-63825707 | None:intergenic | 30.0% | |
| CAACATGGTATCATTAACAT+AGG | + | chr1_3:63826046-63826065 | None:intergenic | 30.0% | |
| TTGATATGATGAACATATGC+AGG | - | chr1_3:63826063-63826082 | Msa0132610:CDS | 30.0% | |
| GACATCAACATTAACAAAGA+TGG | - | chr1_3:63826105-63826124 | Msa0132610:intron | 30.0% | |
| ! | ATTTTCAGCTCTTTCTTGAA+TGG | + | chr1_3:63826183-63826202 | None:intergenic | 30.0% |
| TATCCAAAACAACTCCATTA+GGG | + | chr1_3:63826233-63826252 | None:intergenic | 30.0% | |
| TTATCCAAAACAACTCCATT+AGG | + | chr1_3:63826234-63826253 | None:intergenic | 30.0% | |
| GTTTCTTTAGATAAGAAGAC+AGG | + | chr1_3:63825688-63825707 | None:intergenic | 30.0% | |
| CAACATGGTATCATTAACAT+AGG | + | chr1_3:63826046-63826065 | None:intergenic | 30.0% | |
| TTGATATGATGAACATATGC+AGG | - | chr1_3:63826063-63826082 | Msa0132610:CDS | 30.0% | |
| GACATCAACATTAACAAAGA+TGG | - | chr1_3:63826105-63826124 | Msa0132610:intron | 30.0% | |
| ! | ATTTTCAGCTCTTTCTTGAA+TGG | + | chr1_3:63826183-63826202 | None:intergenic | 30.0% |
| TATCCAAAACAACTCCATTA+GGG | + | chr1_3:63826233-63826252 | None:intergenic | 30.0% | |
| TTATCCAAAACAACTCCATT+AGG | + | chr1_3:63826234-63826253 | None:intergenic | 30.0% | |
| CTGAAAATCAGAAATGGAAG+TGG | + | chr1_3:63825644-63825663 | None:intergenic | 35.0% | |
| GGCAAACTGAAAATCAGAAA+TGG | + | chr1_3:63825650-63825669 | None:intergenic | 35.0% | |
| ! | TTTTCACCAGCTATCATGAA+GGG | + | chr1_3:63825904-63825923 | None:intergenic | 35.0% |
| GGTACAAAAATTTCAGTGAG+TGG | - | chr1_3:63826126-63826145 | Msa0132610:intron | 35.0% | |
| ! | AATCCCTAATGGAGTTGTTT+TGG | - | chr1_3:63826227-63826246 | Msa0132610:intron | 35.0% |
| CAAAGCTAAGTATTACGAAG+AGG | - | chr1_3:63826257-63826276 | Msa0132610:intron | 35.0% | |
| GAGAATAGTGATGCCAAAAA+CGG | - | chr1_3:63826290-63826309 | Msa0132610:intron | 35.0% | |
| !!! | CCTTTTGCTATTTCCGTTTT+TGG | + | chr1_3:63826306-63826325 | None:intergenic | 35.0% |
| CCAAAAACGGAAATAGCAAA+AGG | - | chr1_3:63826303-63826322 | Msa0132610:intron | 35.0% | |
| ! | TCTTCTCTTGCATTTTGTTC+TGG | + | chr1_3:63826396-63826415 | None:intergenic | 35.0% |
| TACAGATGATAAGAGTGAAG+AGG | - | chr1_3:63826425-63826444 | Msa0132610:intron | 35.0% | |
| CTGAAAATCAGAAATGGAAG+TGG | + | chr1_3:63825644-63825663 | None:intergenic | 35.0% | |
| GGCAAACTGAAAATCAGAAA+TGG | + | chr1_3:63825650-63825669 | None:intergenic | 35.0% | |
| ! | TTTTCACCAGCTATCATGAA+GGG | + | chr1_3:63825904-63825923 | None:intergenic | 35.0% |
| GGTACAAAAATTTCAGTGAG+TGG | - | chr1_3:63826126-63826145 | Msa0132610:intron | 35.0% | |
| ! | AATCCCTAATGGAGTTGTTT+TGG | - | chr1_3:63826227-63826246 | Msa0132610:intron | 35.0% |
| CAAAGCTAAGTATTACGAAG+AGG | - | chr1_3:63826257-63826276 | Msa0132610:intron | 35.0% | |
| GAGAATAGTGATGCCAAAAA+CGG | - | chr1_3:63826290-63826309 | Msa0132610:intron | 35.0% | |
| !!! | CCTTTTGCTATTTCCGTTTT+TGG | + | chr1_3:63826306-63826325 | None:intergenic | 35.0% |
| CCAAAAACGGAAATAGCAAA+AGG | - | chr1_3:63826303-63826322 | Msa0132610:intron | 35.0% | |
| ! | TCTTCTCTTGCATTTTGTTC+TGG | + | chr1_3:63826396-63826415 | None:intergenic | 35.0% |
| TACAGATGATAAGAGTGAAG+AGG | - | chr1_3:63826425-63826444 | Msa0132610:intron | 35.0% | |
| ! | GTTTTCACCAGCTATCATGA+AGG | + | chr1_3:63825905-63825924 | None:intergenic | 40.0% |
| GCTAAGTATTACGAAGAGGA+AGG | - | chr1_3:63826261-63826280 | Msa0132610:intron | 40.0% | |
| !! | ATAGCAAAAGGAGTACTGAG+TGG | - | chr1_3:63826315-63826334 | Msa0132610:intron | 40.0% |
| !! | TAGCAAAAGGAGTACTGAGT+GGG | - | chr1_3:63826316-63826335 | Msa0132610:intron | 40.0% |
| AGAAGAAGTGAAGGAAGAAG+AGG | - | chr1_3:63826347-63826366 | Msa0132610:intron | 40.0% | |
| GAAGAAGTGAAGGAAGAAGA+GGG | - | chr1_3:63826348-63826367 | Msa0132610:intron | 40.0% | |
| ! | GTTTTCACCAGCTATCATGA+AGG | + | chr1_3:63825905-63825924 | None:intergenic | 40.0% |
| GCTAAGTATTACGAAGAGGA+AGG | - | chr1_3:63826261-63826280 | Msa0132610:intron | 40.0% | |
| !! | ATAGCAAAAGGAGTACTGAG+TGG | - | chr1_3:63826315-63826334 | Msa0132610:intron | 40.0% |
| !! | TAGCAAAAGGAGTACTGAGT+GGG | - | chr1_3:63826316-63826335 | Msa0132610:intron | 40.0% |
| AGAAGAAGTGAAGGAAGAAG+AGG | - | chr1_3:63826347-63826366 | Msa0132610:intron | 40.0% | |
| GAAGAAGTGAAGGAAGAAGA+GGG | - | chr1_3:63826348-63826367 | Msa0132610:intron | 40.0% | |
| !! | ACCTTTGAGATGTGCTGTGA+GGG | + | chr1_3:63825725-63825744 | None:intergenic | 45.0% |
| !! | AACCTTTGAGATGTGCTGTG+AGG | + | chr1_3:63825726-63825745 | None:intergenic | 45.0% |
| CCACTTGTAAGACTGTTCGA+CGG | - | chr1_3:63825820-63825839 | Msa0132610:CDS | 45.0% | |
| CATAAGCCCTTCATGATAGC+TGG | - | chr1_3:63825895-63825914 | Msa0132610:CDS | 45.0% | |
| !! | AGCAAAAGGAGTACTGAGTG+GGG | - | chr1_3:63826317-63826336 | Msa0132610:intron | 45.0% |
| GGTGGGAATAGAAGAAGTGA+AGG | - | chr1_3:63826338-63826357 | Msa0132610:intron | 45.0% | |
| CTAGGACGTCTCTTCTTAAC+AGG | + | chr1_3:63826459-63826478 | None:intergenic | 45.0% | |
| AGAGACGTCCTAGAAAACCA+TGG | - | chr1_3:63826466-63826485 | Msa0132610:CDS | 45.0% | |
| ! | CAAGGTTGCCATGGTTTTCT+AGG | + | chr1_3:63826477-63826496 | None:intergenic | 45.0% |
| GTTGATCCTCCAAGAACCAA+AGG | + | chr1_3:63826504-63826523 | None:intergenic | 45.0% | |
| !! | ACCTTTGAGATGTGCTGTGA+GGG | + | chr1_3:63825725-63825744 | None:intergenic | 45.0% |
| !! | AACCTTTGAGATGTGCTGTG+AGG | + | chr1_3:63825726-63825745 | None:intergenic | 45.0% |
| CCACTTGTAAGACTGTTCGA+CGG | - | chr1_3:63825820-63825839 | Msa0132610:CDS | 45.0% | |
| CATAAGCCCTTCATGATAGC+TGG | - | chr1_3:63825895-63825914 | Msa0132610:CDS | 45.0% | |
| !! | AGCAAAAGGAGTACTGAGTG+GGG | - | chr1_3:63826317-63826336 | Msa0132610:intron | 45.0% |
| GGTGGGAATAGAAGAAGTGA+AGG | - | chr1_3:63826338-63826357 | Msa0132610:intron | 45.0% | |
| CTAGGACGTCTCTTCTTAAC+AGG | + | chr1_3:63826459-63826478 | None:intergenic | 45.0% | |
| AGAGACGTCCTAGAAAACCA+TGG | - | chr1_3:63826466-63826485 | Msa0132610:CDS | 45.0% | |
| ! | CAAGGTTGCCATGGTTTTCT+AGG | + | chr1_3:63826477-63826496 | None:intergenic | 45.0% |
| GTTGATCCTCCAAGAACCAA+AGG | + | chr1_3:63826504-63826523 | None:intergenic | 45.0% | |
| CAGTTTGCCAAAGACAGAGC+AGG | - | chr1_3:63825661-63825680 | Msa0132610:CDS | 50.0% | |
| ACCCTCACAGCACATCTCAA+AGG | - | chr1_3:63825721-63825740 | Msa0132610:CDS | 50.0% | |
| CCGTCGAACAGTCTTACAAG+TGG | + | chr1_3:63825823-63825842 | None:intergenic | 50.0% | |
| !! | AAAAGGAGTACTGAGTGGGG+TGG | - | chr1_3:63826320-63826339 | Msa0132610:intron | 50.0% |
| !! | AAAGGAGTACTGAGTGGGGT+GGG | - | chr1_3:63826321-63826340 | Msa0132610:intron | 50.0% |
| AAAGGTGGACAAGGTTGCCA+TGG | + | chr1_3:63826486-63826505 | None:intergenic | 50.0% | |
| ATGGCAACCTTGTCCACCTT+TGG | - | chr1_3:63826485-63826504 | Msa0132610:CDS | 50.0% | |
| CCAAGAACCAAAGGTGGACA+AGG | + | chr1_3:63826495-63826514 | None:intergenic | 50.0% | |
| !! | CCTTGTCCACCTTTGGTTCT+TGG | - | chr1_3:63826492-63826511 | Msa0132610:CDS | 50.0% |
| !! | TGTCCACCTTTGGTTCTTGG+AGG | - | chr1_3:63826495-63826514 | Msa0132610:CDS | 50.0% |
| GATCCTCCAAGAACCAAAGG+TGG | + | chr1_3:63826501-63826520 | None:intergenic | 50.0% | |
| CAGTTTGCCAAAGACAGAGC+AGG | - | chr1_3:63825661-63825680 | Msa0132610:CDS | 50.0% | |
| ACCCTCACAGCACATCTCAA+AGG | - | chr1_3:63825721-63825740 | Msa0132610:CDS | 50.0% | |
| CCGTCGAACAGTCTTACAAG+TGG | + | chr1_3:63825823-63825842 | None:intergenic | 50.0% | |
| !! | AAAAGGAGTACTGAGTGGGG+TGG | - | chr1_3:63826320-63826339 | Msa0132610:intron | 50.0% |
| !! | AAAGGAGTACTGAGTGGGGT+GGG | - | chr1_3:63826321-63826340 | Msa0132610:intron | 50.0% |
| AAAGGTGGACAAGGTTGCCA+TGG | + | chr1_3:63826486-63826505 | None:intergenic | 50.0% | |
| ATGGCAACCTTGTCCACCTT+TGG | - | chr1_3:63826485-63826504 | Msa0132610:CDS | 50.0% | |
| CCAAGAACCAAAGGTGGACA+AGG | + | chr1_3:63826495-63826514 | None:intergenic | 50.0% | |
| !! | CCTTGTCCACCTTTGGTTCT+TGG | - | chr1_3:63826492-63826511 | Msa0132610:CDS | 50.0% |
| !! | TGTCCACCTTTGGTTCTTGG+AGG | - | chr1_3:63826495-63826514 | Msa0132610:CDS | 50.0% |
| GATCCTCCAAGAACCAAAGG+TGG | + | chr1_3:63826501-63826520 | None:intergenic | 50.0% | |
| GACAGGTCCTGCTCTGTCTT+TGG | + | chr1_3:63825671-63825690 | None:intergenic | 55.0% | |
| GACAGGTCCTGCTCTGTCTT+TGG | + | chr1_3:63825671-63825690 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_3 | gene | 63825595 | 63826581 | 63825595 | ID=Msa0132610;Name=Msa0132610 |
| chr1_3 | mRNA | 63825595 | 63826581 | 63825595 | ID=Msa0132610-mRNA-1;Parent=Msa0132610;Name=Msa0132610-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|1|0|1|1|1|2|0|214 |
| chr1_3 | exon | 63826434 | 63826581 | 63826434 | ID=Msa0132610-mRNA-1:exon:21356;Parent=Msa0132610-mRNA-1 |
| chr1_3 | exon | 63825595 | 63826091 | 63825595 | ID=Msa0132610-mRNA-1:exon:21355;Parent=Msa0132610-mRNA-1 |
| chr1_3 | CDS | 63826434 | 63826581 | 63826434 | ID=Msa0132610-mRNA-1:cds;Parent=Msa0132610-mRNA-1 |
| chr1_3 | CDS | 63825595 | 63826091 | 63825595 | ID=Msa0132610-mRNA-1:cds;Parent=Msa0132610-mRNA-1 |
| Gene Sequence |
| Protein sequence |
