Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0577880 | XP_003592099.1 | 95.327 | 214 | 10 | 0 | 1 | 214 | 1 | 214 | 2.38e-145 | 413 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0577880 | G7IBZ6 | 95.327 | 214 | 10 | 0 | 1 | 214 | 1 | 214 | 1.14e-145 | 413 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0132610 | Msa0577880 | 0.904632 | 1.002269e-79 | -8.615850e-47 |
| Msa0577880 | Msa1071770 | 0.821778 | 3.369166e-53 | -8.615850e-47 |
| Msa0577880 | Msa1158830 | 0.811822 | 5.776272e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0577880 | MtrunA17_Chr1g0201801 | 95.327 | 214 | 10 | 0 | 1 | 214 | 1 | 214 | 2.19e-149 | 413 |
| Msa0577880 | MtrunA17_Chr6g0464341 | 56.566 | 99 | 37 | 3 | 50 | 145 | 36 | 131 | 8.43e-23 | 95.9 |
| Msa0577880 | MtrunA17_Chr6g0472121 | 59.184 | 98 | 34 | 3 | 50 | 144 | 9 | 103 | 1.16e-22 | 95.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0577880 | AT1G76770.1 | 55.118 | 127 | 54 | 1 | 1 | 124 | 1 | 127 | 1.78e-40 | 138 |
| Msa0577880 | AT1G76780.3 | 51.181 | 127 | 59 | 1 | 1 | 124 | 1 | 127 | 6.89e-35 | 131 |
| Msa0577880 | AT1G76780.2 | 51.181 | 127 | 59 | 1 | 1 | 124 | 1 | 127 | 6.89e-35 | 131 |
Find 35 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTGTCCACCTTTGGTTCT+TGG | 0.114619 | 4_1:+82197118 | Msa0577880:CDS |
| AATCCCTAATGGAGTTGTTT+TGG | 0.253932 | 4_1:+82196853 | Msa0577880:CDS |
| CTAGGACGTCTCTTCTTAAC+AGG | 0.266778 | 4_1:-82197082 | None:intergenic |
| TTATCCAAAACAACTCCATT+AGG | 0.283059 | 4_1:-82196857 | None:intergenic |
| AAGAAAGAGCTGAAAATAAA+AGG | 0.299279 | 4_1:+82196812 | Msa0577880:CDS |
| GACAGGTCCTGCTCTGTCTT+TGG | 0.343396 | 4_1:-82196294 | None:intergenic |
| TATCCAAAACAACTCCATTA+GGG | 0.355864 | 4_1:-82196856 | None:intergenic |
| ATTGCTATGGAGGTTGAGTT+AGG | 0.385137 | 4_1:+82196215 | None:intergenic |
| ATGGCAACCTTGTCCACCTT+TGG | 0.390972 | 4_1:+82197111 | Msa0577880:CDS |
| AAGAAGTTTAAAATCCCTAA+TGG | 0.416386 | 4_1:+82196842 | Msa0577880:CDS |
| TGTCCACCTTTGGTTCTTGG+AGG | 0.424444 | 4_1:+82197121 | Msa0577880:CDS |
| GGCAAACTGAAAATCAGAAA+TGG | 0.432210 | 4_1:-82196273 | None:intergenic |
| CAGTTTGCCAAAGACAGAGC+AGG | 0.497784 | 4_1:+82196287 | Msa0577880:CDS |
| AGAAGAAGTGAAGGAAGAAG+AGG | 0.509148 | 4_1:+82196973 | Msa0577880:CDS |
| GTTTCTTTAGATAAGAAGAC+AGG | 0.514312 | 4_1:-82196311 | None:intergenic |
| GACATCAACATTAACAAAGA+TGG | 0.531815 | 4_1:+82196731 | Msa0577880:CDS |
| CTGAAAATCAGAAATGGAAG+TGG | 0.544169 | 4_1:-82196267 | None:intergenic |
| GAAGAAGTGAAGGAAGAAGA+GGG | 0.550743 | 4_1:+82196974 | Msa0577880:CDS |
| CCAAGAACCAAAGGTGGACA+AGG | 0.550847 | 4_1:-82197118 | None:intergenic |
| TAGCAAAAGGAGTACTGAGT+GGG | 0.567184 | 4_1:+82196942 | Msa0577880:CDS |
| TACAGATGATAAGAGTGAAG+AGG | 0.572349 | 4_1:+82197051 | Msa0577880:CDS |
| AAAGGAGTACTGAGTGGGGT+GGG | 0.574936 | 4_1:+82196947 | Msa0577880:CDS |
| ACCTTTGAGATGTGCTGTGA+GGG | 0.582015 | 4_1:-82196348 | None:intergenic |
| GCTAAGTATTACGAAGAGGA+AGG | 0.582114 | 4_1:+82196887 | Msa0577880:CDS |
| ACCCTCACAGCACATCTCAA+AGG | 0.582279 | 4_1:+82196347 | Msa0577880:CDS |
| AAAGGTGGACAAGGTTGCCA+TGG | 0.584568 | 4_1:-82197109 | None:intergenic |
| CAAAGCTAAGTATTACGAAG+AGG | 0.607521 | 4_1:+82196883 | Msa0577880:CDS |
| GGTGGGAATAGAAGAAGTGA+AGG | 0.616063 | 4_1:+82196964 | Msa0577880:CDS |
| AAAAGGAGTACTGAGTGGGG+TGG | 0.620358 | 4_1:+82196946 | Msa0577880:CDS |
| GTTGATCCTCCAAGAACCAA+AGG | 0.621557 | 4_1:-82197127 | None:intergenic |
| GATCCTCCAAGAACCAAAGG+TGG | 0.669806 | 4_1:-82197124 | None:intergenic |
| AGCAAAAGGAGTACTGAGTG+GGG | 0.702706 | 4_1:+82196943 | Msa0577880:CDS |
| AACCTTTGAGATGTGCTGTG+AGG | 0.714535 | 4_1:-82196349 | None:intergenic |
| AGAGACGTCCTAGAAAACCA+TGG | 0.719572 | 4_1:+82197092 | Msa0577880:CDS |
| ATAGCAAAAGGAGTACTGAG+TGG | 0.745850 | 4_1:+82196941 | Msa0577880:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CATCTTATGATATGTCTATA+AGG | + | chr4_1:82196481-82196500 | Msa0577880:intron | 25.0% |
| ! | ATATGTTCATCATATCAACA+TGG | - | chr4_1:82196687-82196706 | None:intergenic | 25.0% |
| ! | AAGAAAGAGCTGAAAATAAA+AGG | + | chr4_1:82196812-82196831 | Msa0577880:CDS | 25.0% |
| !! | AAGAAGTTTAAAATCCCTAA+TGG | + | chr4_1:82196842-82196861 | Msa0577880:CDS | 25.0% |
| ! | CATCTTATGATATGTCTATA+AGG | + | chr4_1:82196481-82196500 | Msa0577880:intron | 25.0% |
| ! | ATATGTTCATCATATCAACA+TGG | - | chr4_1:82196687-82196706 | None:intergenic | 25.0% |
| ! | AAGAAAGAGCTGAAAATAAA+AGG | + | chr4_1:82196812-82196831 | Msa0577880:CDS | 25.0% |
| !! | AAGAAGTTTAAAATCCCTAA+TGG | + | chr4_1:82196842-82196861 | Msa0577880:CDS | 25.0% |
| GTTTCTTTAGATAAGAAGAC+AGG | - | chr4_1:82196314-82196333 | None:intergenic | 30.0% | |
| CAACATGGTATCATTAACAT+AGG | - | chr4_1:82196672-82196691 | None:intergenic | 30.0% | |
| TTGATATGATGAACATATGC+AGG | + | chr4_1:82196689-82196708 | Msa0577880:intron | 30.0% | |
| GACATCAACATTAACAAAGA+TGG | + | chr4_1:82196731-82196750 | Msa0577880:CDS | 30.0% | |
| ! | ATTTTCAGCTCTTTCTTGAA+TGG | - | chr4_1:82196809-82196828 | None:intergenic | 30.0% |
| TATCCAAAACAACTCCATTA+GGG | - | chr4_1:82196859-82196878 | None:intergenic | 30.0% | |
| TTATCCAAAACAACTCCATT+AGG | - | chr4_1:82196860-82196879 | None:intergenic | 30.0% | |
| GAAAATAGTGATGCCAAAAA+CGG | + | chr4_1:82196916-82196935 | Msa0577880:CDS | 30.0% | |
| GTTTCTTTAGATAAGAAGAC+AGG | - | chr4_1:82196314-82196333 | None:intergenic | 30.0% | |
| CAACATGGTATCATTAACAT+AGG | - | chr4_1:82196672-82196691 | None:intergenic | 30.0% | |
| TTGATATGATGAACATATGC+AGG | + | chr4_1:82196689-82196708 | Msa0577880:intron | 30.0% | |
| GACATCAACATTAACAAAGA+TGG | + | chr4_1:82196731-82196750 | Msa0577880:CDS | 30.0% | |
| ! | ATTTTCAGCTCTTTCTTGAA+TGG | - | chr4_1:82196809-82196828 | None:intergenic | 30.0% |
| TATCCAAAACAACTCCATTA+GGG | - | chr4_1:82196859-82196878 | None:intergenic | 30.0% | |
| TTATCCAAAACAACTCCATT+AGG | - | chr4_1:82196860-82196879 | None:intergenic | 30.0% | |
| GAAAATAGTGATGCCAAAAA+CGG | + | chr4_1:82196916-82196935 | Msa0577880:CDS | 30.0% | |
| CTGAAAATCAGAAATGGAAG+TGG | - | chr4_1:82196270-82196289 | None:intergenic | 35.0% | |
| GGCAAACTGAAAATCAGAAA+TGG | - | chr4_1:82196276-82196295 | None:intergenic | 35.0% | |
| ! | TTTTCACCAGCTATCATGAA+GGG | - | chr4_1:82196530-82196549 | None:intergenic | 35.0% |
| GGTACAAAAATTTCAGTGAG+TGG | + | chr4_1:82196752-82196771 | Msa0577880:CDS | 35.0% | |
| ! | AATCCCTAATGGAGTTGTTT+TGG | + | chr4_1:82196853-82196872 | Msa0577880:CDS | 35.0% |
| CAAAGCTAAGTATTACGAAG+AGG | + | chr4_1:82196883-82196902 | Msa0577880:CDS | 35.0% | |
| !!! | CCTTTTGCTATTTCCGTTTT+TGG | - | chr4_1:82196932-82196951 | None:intergenic | 35.0% |
| CCAAAAACGGAAATAGCAAA+AGG | + | chr4_1:82196929-82196948 | Msa0577880:CDS | 35.0% | |
| ! | TCTTCTCTTGCATTTTGTTC+TGG | - | chr4_1:82197022-82197041 | None:intergenic | 35.0% |
| TACAGATGATAAGAGTGAAG+AGG | + | chr4_1:82197051-82197070 | Msa0577880:CDS | 35.0% | |
| CTGAAAATCAGAAATGGAAG+TGG | - | chr4_1:82196270-82196289 | None:intergenic | 35.0% | |
| GGCAAACTGAAAATCAGAAA+TGG | - | chr4_1:82196276-82196295 | None:intergenic | 35.0% | |
| ! | TTTTCACCAGCTATCATGAA+GGG | - | chr4_1:82196530-82196549 | None:intergenic | 35.0% |
| GGTACAAAAATTTCAGTGAG+TGG | + | chr4_1:82196752-82196771 | Msa0577880:CDS | 35.0% | |
| ! | AATCCCTAATGGAGTTGTTT+TGG | + | chr4_1:82196853-82196872 | Msa0577880:CDS | 35.0% |
| CAAAGCTAAGTATTACGAAG+AGG | + | chr4_1:82196883-82196902 | Msa0577880:CDS | 35.0% | |
| !!! | CCTTTTGCTATTTCCGTTTT+TGG | - | chr4_1:82196932-82196951 | None:intergenic | 35.0% |
| CCAAAAACGGAAATAGCAAA+AGG | + | chr4_1:82196929-82196948 | Msa0577880:CDS | 35.0% | |
| ! | TCTTCTCTTGCATTTTGTTC+TGG | - | chr4_1:82197022-82197041 | None:intergenic | 35.0% |
| TACAGATGATAAGAGTGAAG+AGG | + | chr4_1:82197051-82197070 | Msa0577880:CDS | 35.0% | |
| ! | GTTTTCACCAGCTATCATGA+AGG | - | chr4_1:82196531-82196550 | None:intergenic | 40.0% |
| GCTAAGTATTACGAAGAGGA+AGG | + | chr4_1:82196887-82196906 | Msa0577880:CDS | 40.0% | |
| !! | ATAGCAAAAGGAGTACTGAG+TGG | + | chr4_1:82196941-82196960 | Msa0577880:CDS | 40.0% |
| !! | TAGCAAAAGGAGTACTGAGT+GGG | + | chr4_1:82196942-82196961 | Msa0577880:CDS | 40.0% |
| AGAAGAAGTGAAGGAAGAAG+AGG | + | chr4_1:82196973-82196992 | Msa0577880:CDS | 40.0% | |
| GAAGAAGTGAAGGAAGAAGA+GGG | + | chr4_1:82196974-82196993 | Msa0577880:CDS | 40.0% | |
| ! | GTTTTCACCAGCTATCATGA+AGG | - | chr4_1:82196531-82196550 | None:intergenic | 40.0% |
| GCTAAGTATTACGAAGAGGA+AGG | + | chr4_1:82196887-82196906 | Msa0577880:CDS | 40.0% | |
| !! | ATAGCAAAAGGAGTACTGAG+TGG | + | chr4_1:82196941-82196960 | Msa0577880:CDS | 40.0% |
| !! | TAGCAAAAGGAGTACTGAGT+GGG | + | chr4_1:82196942-82196961 | Msa0577880:CDS | 40.0% |
| AGAAGAAGTGAAGGAAGAAG+AGG | + | chr4_1:82196973-82196992 | Msa0577880:CDS | 40.0% | |
| GAAGAAGTGAAGGAAGAAGA+GGG | + | chr4_1:82196974-82196993 | Msa0577880:CDS | 40.0% | |
| !! | ACCTTTGAGATGTGCTGTGA+GGG | - | chr4_1:82196351-82196370 | None:intergenic | 45.0% |
| !! | AACCTTTGAGATGTGCTGTG+AGG | - | chr4_1:82196352-82196371 | None:intergenic | 45.0% |
| CCACTTGTAAGACTGTTCGA+CGG | + | chr4_1:82196446-82196465 | Msa0577880:intron | 45.0% | |
| CATAAGCCCTTCATGATAGC+TGG | + | chr4_1:82196521-82196540 | Msa0577880:intron | 45.0% | |
| !! | AGCAAAAGGAGTACTGAGTG+GGG | + | chr4_1:82196943-82196962 | Msa0577880:CDS | 45.0% |
| GGTGGGAATAGAAGAAGTGA+AGG | + | chr4_1:82196964-82196983 | Msa0577880:CDS | 45.0% | |
| CTAGGACGTCTCTTCTTAAC+AGG | - | chr4_1:82197085-82197104 | None:intergenic | 45.0% | |
| AGAGACGTCCTAGAAAACCA+TGG | + | chr4_1:82197092-82197111 | Msa0577880:CDS | 45.0% | |
| ! | CAAGGTTGCCATGGTTTTCT+AGG | - | chr4_1:82197103-82197122 | None:intergenic | 45.0% |
| GTTGATCCTCCAAGAACCAA+AGG | - | chr4_1:82197130-82197149 | None:intergenic | 45.0% | |
| !! | ACCTTTGAGATGTGCTGTGA+GGG | - | chr4_1:82196351-82196370 | None:intergenic | 45.0% |
| !! | AACCTTTGAGATGTGCTGTG+AGG | - | chr4_1:82196352-82196371 | None:intergenic | 45.0% |
| CCACTTGTAAGACTGTTCGA+CGG | + | chr4_1:82196446-82196465 | Msa0577880:intron | 45.0% | |
| CATAAGCCCTTCATGATAGC+TGG | + | chr4_1:82196521-82196540 | Msa0577880:intron | 45.0% | |
| !! | AGCAAAAGGAGTACTGAGTG+GGG | + | chr4_1:82196943-82196962 | Msa0577880:CDS | 45.0% |
| GGTGGGAATAGAAGAAGTGA+AGG | + | chr4_1:82196964-82196983 | Msa0577880:CDS | 45.0% | |
| CTAGGACGTCTCTTCTTAAC+AGG | - | chr4_1:82197085-82197104 | None:intergenic | 45.0% | |
| AGAGACGTCCTAGAAAACCA+TGG | + | chr4_1:82197092-82197111 | Msa0577880:CDS | 45.0% | |
| ! | CAAGGTTGCCATGGTTTTCT+AGG | - | chr4_1:82197103-82197122 | None:intergenic | 45.0% |
| GTTGATCCTCCAAGAACCAA+AGG | - | chr4_1:82197130-82197149 | None:intergenic | 45.0% | |
| CAGTTTGCCAAAGACAGAGC+AGG | + | chr4_1:82196287-82196306 | Msa0577880:CDS | 50.0% | |
| ACCCTCACAGCACATCTCAA+AGG | + | chr4_1:82196347-82196366 | Msa0577880:CDS | 50.0% | |
| CCGTCGAACAGTCTTACAAG+TGG | - | chr4_1:82196449-82196468 | None:intergenic | 50.0% | |
| !! | AAAAGGAGTACTGAGTGGGG+TGG | + | chr4_1:82196946-82196965 | Msa0577880:CDS | 50.0% |
| !! | AAAGGAGTACTGAGTGGGGT+GGG | + | chr4_1:82196947-82196966 | Msa0577880:CDS | 50.0% |
| AAAGGTGGACAAGGTTGCCA+TGG | - | chr4_1:82197112-82197131 | None:intergenic | 50.0% | |
| ATGGCAACCTTGTCCACCTT+TGG | + | chr4_1:82197111-82197130 | Msa0577880:CDS | 50.0% | |
| CCAAGAACCAAAGGTGGACA+AGG | - | chr4_1:82197121-82197140 | None:intergenic | 50.0% | |
| !! | CCTTGTCCACCTTTGGTTCT+TGG | + | chr4_1:82197118-82197137 | Msa0577880:CDS | 50.0% |
| !! | TGTCCACCTTTGGTTCTTGG+AGG | + | chr4_1:82197121-82197140 | Msa0577880:CDS | 50.0% |
| GATCCTCCAAGAACCAAAGG+TGG | - | chr4_1:82197127-82197146 | None:intergenic | 50.0% | |
| CAGTTTGCCAAAGACAGAGC+AGG | + | chr4_1:82196287-82196306 | Msa0577880:CDS | 50.0% | |
| ACCCTCACAGCACATCTCAA+AGG | + | chr4_1:82196347-82196366 | Msa0577880:CDS | 50.0% | |
| CCGTCGAACAGTCTTACAAG+TGG | - | chr4_1:82196449-82196468 | None:intergenic | 50.0% | |
| !! | AAAAGGAGTACTGAGTGGGG+TGG | + | chr4_1:82196946-82196965 | Msa0577880:CDS | 50.0% |
| !! | AAAGGAGTACTGAGTGGGGT+GGG | + | chr4_1:82196947-82196966 | Msa0577880:CDS | 50.0% |
| AAAGGTGGACAAGGTTGCCA+TGG | - | chr4_1:82197112-82197131 | None:intergenic | 50.0% | |
| ATGGCAACCTTGTCCACCTT+TGG | + | chr4_1:82197111-82197130 | Msa0577880:CDS | 50.0% | |
| CCAAGAACCAAAGGTGGACA+AGG | - | chr4_1:82197121-82197140 | None:intergenic | 50.0% | |
| !! | CCTTGTCCACCTTTGGTTCT+TGG | + | chr4_1:82197118-82197137 | Msa0577880:CDS | 50.0% |
| !! | TGTCCACCTTTGGTTCTTGG+AGG | + | chr4_1:82197121-82197140 | Msa0577880:CDS | 50.0% |
| GATCCTCCAAGAACCAAAGG+TGG | - | chr4_1:82197127-82197146 | None:intergenic | 50.0% | |
| GACAGGTCCTGCTCTGTCTT+TGG | - | chr4_1:82196297-82196316 | None:intergenic | 55.0% | |
| GACAGGTCCTGCTCTGTCTT+TGG | - | chr4_1:82196297-82196316 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 82196221 | 82197207 | 82196221 | ID=Msa0577880;Name=Msa0577880 |
| chr4_1 | mRNA | 82196221 | 82197207 | 82196221 | ID=Msa0577880-mRNA-1;Parent=Msa0577880;Name=Msa0577880-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|1|0|1|1|1|2|0|214 |
| chr4_1 | exon | 82196221 | 82196368 | 82196221 | ID=Msa0577880-mRNA-1:exon:29738;Parent=Msa0577880-mRNA-1 |
| chr4_1 | exon | 82196711 | 82197207 | 82196711 | ID=Msa0577880-mRNA-1:exon:29739;Parent=Msa0577880-mRNA-1 |
| chr4_1 | CDS | 82196221 | 82196368 | 82196221 | ID=Msa0577880-mRNA-1:cds;Parent=Msa0577880-mRNA-1 |
| chr4_1 | CDS | 82196711 | 82197207 | 82196711 | ID=Msa0577880-mRNA-1:cds;Parent=Msa0577880-mRNA-1 |
| Gene Sequence |
| Protein sequence |
