AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0132970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0132970 MtrunA17_Chr1g0202381 83.444 151 7 1 6 138 635 785 2.14e-70 225
Msa0132970 MtrunA17_Chr1g0204031 67.550 151 31 1 6 138 724 874 1.90e-53 180
Msa0132970 MtrunA17_Chr5g0397121 61.184 152 38 4 6 138 679 828 9.21e-50 169
Msa0132970 MtrunA17_Chr1g0202371 68.212 151 30 1 6 138 563 713 3.71e-49 167
Msa0132970 MtrunA17_Chr6g0462581 62.416 149 39 2 6 138 666 813 3.66e-48 164
Msa0132970 MtrunA17_Chr8g0387641 50.617 162 50 4 6 138 687 847 2.38e-43 151
Msa0132970 MtrunA17_Chr1g0195731 46.429 140 67 4 6 138 1101 1239 1.91e-33 123
Msa0132970 MtrunA17_Chr1g0195741 46.429 140 67 5 6 138 1105 1243 5.14e-32 119
Msa0132970 MtrunA17_Chr4g0038101 42.857 126 66 2 6 130 888 1008 1.21e-27 106
Msa0132970 MtrunA17_Chr3g0116131 46.763 139 66 4 6 137 1069 1206 2.10e-27 106
Msa0132970 MtrunA17_Chr1g0204061 72.131 61 17 0 78 138 2 62 1.82e-24 89.4
Msa0132970 MtrunA17_Chr1g0204051 76.316 38 9 0 6 43 38 75 9.84e-15 64.7
Msa0132970 MtrunA17_Chr3g0127401 42.857 77 39 2 2 73 239 315 6.30e-13 64.3
Msa0132970 MtrunA17_Chr3g0127431 37.778 90 50 3 2 85 242 331 8.49e-13 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0132970 AT4G28000.2 56.954 151 46 2 6 138 683 832 1.84e-47 163
Msa0132970 AT4G28000.1 56.954 151 46 2 6 138 681 830 1.90e-47 163
Msa0132970 AT1G64110.2 57.143 147 48 4 6 138 684 829 1.62e-45 157
Msa0132970 AT1G64110.4 57.143 147 48 4 6 138 684 829 1.62e-45 157
Msa0132970 AT1G64110.1 57.143 147 48 4 6 138 679 824 1.76e-45 157
Msa0132970 AT5G52882.1 53.642 151 51 2 6 138 680 829 1.82e-45 157
Msa0132970 AT1G64110.5 57.143 147 48 4 6 138 682 827 1.87e-45 157
Msa0132970 AT1G64110.3 57.143 147 48 4 6 138 682 827 1.87e-45 157
Msa0132970 AT1G02890.2 45.000 140 69 4 6 138 1080 1218 1.93e-32 120
Msa0132970 AT1G02890.1 45.324 139 68 4 6 137 1108 1245 2.07e-32 120
Msa0132970 AT4G02480.1 44.604 139 69 4 6 137 1127 1264 1.26e-29 112
Msa0132970 AT4G28000.3 56.842 95 41 0 6 100 683 777 7.61e-29 110
Msa0132970 AT4G24860.4 45.324 139 68 4 6 137 957 1094 1.04e-27 107
Msa0132970 AT4G24860.2 45.324 139 68 4 6 137 980 1117 1.04e-27 107
Msa0132970 AT4G24860.1 45.324 139 68 4 6 137 984 1121 1.13e-27 107
Msa0132970 AT4G24860.3 45.324 139 68 4 6 137 982 1119 1.21e-27 107
Msa0132970 AT1G50140.2 47.619 126 60 3 6 130 856 976 4.50e-27 105
Msa0132970 AT1G50140.5 47.619 126 60 3 6 130 856 976 4.50e-27 105
Msa0132970 AT1G50140.3 47.619 126 60 3 6 130 878 998 4.69e-27 105
Msa0132970 AT1G50140.1 47.619 126 60 3 6 130 878 998 4.69e-27 105
Msa0132970 AT1G50140.4 47.619 126 60 3 6 130 878 998 4.69e-27 105
Msa0132970 AT3G19740.1 46.512 129 57 4 6 130 868 988 5.88e-26 102
Msa0132970 AT1G62130.2 39.716 141 72 4 6 136 898 1035 1.53e-24 98.2
Msa0132970 AT1G62130.1 39.716 141 72 4 6 136 905 1042 1.58e-24 98.2
Msa0132970 AT5G53540.1 35.780 109 60 5 2 104 242 346 6.72e-13 64.7
Msa0132970 AT4G27680.1 31.387 137 75 7 2 132 239 362 2.30e-11 60.5

Find 32 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATGTGTCCGTATTAAAAT+TGG 0.218812 1_3:+64224827 None:intergenic
TATCTATACTGAATGATTAA+TGG 0.260519 1_3:+64224775 None:intergenic
CACTATTGACAAATATATTT+CGG 0.268771 1_3:+64224681 None:intergenic
CCTGTTGTATTAGTTCCTTA+AGG 0.295010 1_3:+64226049 None:intergenic
CTGTTGTATTAGTTCCTTAA+GGG 0.298402 1_3:+64226050 None:intergenic
GGCTTAAAATATACATCCTT+TGG 0.353279 1_3:+64224796 None:intergenic
GTCTTCCGTCTGCAGAAAAC+AGG 0.355275 1_3:-64226285 Msa0132970:CDS
TGTTGACCCGTGCGCCGCAC+GGG 0.461472 1_3:-64224713 Msa0132970:three_prime_UTR
AAGGAATGGAATGATTTGTA+TGG 0.463735 1_3:-64224909 Msa0132970:CDS
ATATAGTGGAAGTGATCTTA+AGG 0.469929 1_3:-64226182 Msa0132970:intron
TGTATGGAGAAGGAGGTTCA+AGG 0.471721 1_3:-64224893 Msa0132970:CDS
ATCAACAATGACCAGGTTGA+AGG 0.500442 1_3:-64224928 Msa0132970:CDS
TGGAATGATTTGTATGGAGA+AGG 0.503022 1_3:-64224903 Msa0132970:CDS
CAATGACCAGGTTGAAGGAA+TGG 0.505602 1_3:-64224923 Msa0132970:CDS
CCTTAAGGAACTAATACAAC+AGG 0.508742 1_3:-64226049 Msa0132970:CDS
TTTATCTTCTTCAGTAGCAA+TGG 0.511349 1_3:+64225876 None:intergenic
TTGTTGACCCGTGCGCCGCA+CGG 0.514648 1_3:-64224714 Msa0132970:three_prime_UTR
AACAGACCCGTGCGGCGCAC+GGG 0.519535 1_3:+64224707 None:intergenic
AAACAGACCCGTGCGGCGCA+CGG 0.528470 1_3:+64224706 None:intergenic
ACTATGACAGAAGGATATAG+TGG 0.531817 1_3:-64226196 Msa0132970:CDS
AGGAGGTTCAAGGAAGAAAG+AGG 0.570259 1_3:-64224883 Msa0132970:CDS
GATATGCTGCAGTCATGCAC+AGG 0.577750 1_3:+64226076 None:intergenic
AATGATTTGTATGGAGAAGG+AGG 0.581152 1_3:-64224900 Msa0132970:CDS
ATCATTCCATTCCTTCAACC+TGG 0.581868 1_3:+64224917 None:intergenic
GGAACTAATACAACAGGAGA+AGG 0.585212 1_3:-64226043 Msa0132970:CDS
CAGAAGGATCAACAATGACC+AGG 0.586376 1_3:-64224935 Msa0132970:CDS
TGTTGTATTAGTTCCTTAAG+GGG 0.594732 1_3:+64226051 None:intergenic
GAGCTTTCAACTATGACAGA+AGG 0.605225 1_3:-64226205 Msa0132970:CDS
ACACATCAATATTTGTCCAA+AGG 0.620109 1_3:-64224812 Msa0132970:three_prime_UTR
GTTGTATTAGTTCCTTAAGG+GGG 0.626882 1_3:+64226052 None:intergenic
TCTTCCGTCTGCAGAAAACA+GGG 0.635804 1_3:-64226284 Msa0132970:CDS
TTCGGCTAAACAGACCCGTG+CGG 0.764236 1_3:+64224699 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTTCTTTTTCTAAAATAAA+TGG + chr1_3:64225034-64225053 None:intergenic 15.0%
!!! ATTTTAGAAAAAGAAGAAAA+AGG - chr1_3:64225037-64225056 Msa0132970:intron 15.0%
!! ATGTAATATTCATTGATAAA+TGG + chr1_3:64225679-64225698 None:intergenic 15.0%
!!! TTTTTATGTATAGAAATTGA+TGG - chr1_3:64225971-64225990 Msa0132970:intron 15.0%
!!! TCTTCTTTTTCTAAAATAAA+TGG + chr1_3:64225034-64225053 None:intergenic 15.0%
!!! ATTTTAGAAAAAGAAGAAAA+AGG - chr1_3:64225037-64225056 Msa0132970:intron 15.0%
!! ATGTAATATTCATTGATAAA+TGG + chr1_3:64225679-64225698 None:intergenic 15.0%
!!! TTTTTATGTATAGAAATTGA+TGG - chr1_3:64225971-64225990 Msa0132970:intron 15.0%
!!! GATCTTAAGGTTAATTTTAT+TGG - chr1_3:64224797-64224816 Msa0132970:three_prime_UTR 20.0%
!! TACTTAAGTACTTATTTGAT+GGG - chr1_3:64225416-64225435 Msa0132970:intron 20.0%
!!! TTTGTCTTTAATATTTTACC+AGG + chr1_3:64225524-64225543 None:intergenic 20.0%
!! AATATTCATTGATAAATGGA+AGG + chr1_3:64225675-64225694 None:intergenic 20.0%
!! AATTTCTTGCAAAAAAAAGA+GGG + chr1_3:64225843-64225862 None:intergenic 20.0%
!! AAATTTCTTGCAAAAAAAAG+AGG + chr1_3:64225844-64225863 None:intergenic 20.0%
!!! AATGATACATTATTTTGTTG+AGG - chr1_3:64225873-64225892 Msa0132970:CDS 20.0%
!! CATAAAAATAATTATTCAGC+TGG + chr1_3:64225959-64225978 None:intergenic 20.0%
!! TATCTATACTGAATGATTAA+TGG + chr1_3:64226194-64226213 None:intergenic 20.0%
!! CACTATTGACAAATATATTT+CGG + chr1_3:64226288-64226307 None:intergenic 20.0%
!!! GATCTTAAGGTTAATTTTAT+TGG - chr1_3:64224797-64224816 Msa0132970:three_prime_UTR 20.0%
!! TACTTAAGTACTTATTTGAT+GGG - chr1_3:64225416-64225435 Msa0132970:intron 20.0%
!!! TTTGTCTTTAATATTTTACC+AGG + chr1_3:64225524-64225543 None:intergenic 20.0%
!! AATATTCATTGATAAATGGA+AGG + chr1_3:64225675-64225694 None:intergenic 20.0%
!! AATTTCTTGCAAAAAAAAGA+GGG + chr1_3:64225843-64225862 None:intergenic 20.0%
!! AAATTTCTTGCAAAAAAAAG+AGG + chr1_3:64225844-64225863 None:intergenic 20.0%
!!! AATGATACATTATTTTGTTG+AGG - chr1_3:64225873-64225892 Msa0132970:CDS 20.0%
!! CATAAAAATAATTATTCAGC+TGG + chr1_3:64225959-64225978 None:intergenic 20.0%
!! TATCTATACTGAATGATTAA+TGG + chr1_3:64226194-64226213 None:intergenic 20.0%
!! CACTATTGACAAATATATTT+CGG + chr1_3:64226288-64226307 None:intergenic 20.0%
! TTTCTATAAGTACTTGCAAA+CGG + chr1_3:64225279-64225298 None:intergenic 25.0%
! CTACTTAAGTACTTATTTGA+TGG - chr1_3:64225415-64225434 Msa0132970:intron 25.0%
! TAAGTTGTTTATCCATACAA+AGG - chr1_3:64225462-64225481 Msa0132970:intron 25.0%
!! GAGGAATTCTTATTTCTTAA+AGG - chr1_3:64225557-64225576 Msa0132970:intron 25.0%
! TAACTTTCATAAGTTATTCC+AGG - chr1_3:64225625-64225644 Msa0132970:intron 25.0%
! AGACATTCATTCTTACAAAT+GGG + chr1_3:64225928-64225947 None:intergenic 25.0%
!!! CAGTTATTTCTTTTAGATTG+TGG - chr1_3:64226113-64226132 Msa0132970:intron 25.0%
! TTTCTATAAGTACTTGCAAA+CGG + chr1_3:64225279-64225298 None:intergenic 25.0%
! CTACTTAAGTACTTATTTGA+TGG - chr1_3:64225415-64225434 Msa0132970:intron 25.0%
! TAAGTTGTTTATCCATACAA+AGG - chr1_3:64225462-64225481 Msa0132970:intron 25.0%
!! GAGGAATTCTTATTTCTTAA+AGG - chr1_3:64225557-64225576 Msa0132970:intron 25.0%
! TAACTTTCATAAGTTATTCC+AGG - chr1_3:64225625-64225644 Msa0132970:intron 25.0%
! AGACATTCATTCTTACAAAT+GGG + chr1_3:64225928-64225947 None:intergenic 25.0%
!!! CAGTTATTTCTTTTAGATTG+TGG - chr1_3:64226113-64226132 Msa0132970:intron 25.0%
! ATATAGTGGAAGTGATCTTA+AGG - chr1_3:64224784-64224803 Msa0132970:three_prime_UTR 30.0%
TGTTGTATTAGTTCCTTAAG+GGG + chr1_3:64224918-64224937 None:intergenic 30.0%
CTGTTGTATTAGTTCCTTAA+GGG + chr1_3:64224919-64224938 None:intergenic 30.0%
TTTATCTTCTTCAGTAGCAA+TGG + chr1_3:64225093-64225112 None:intergenic 30.0%
GACAAACATCATTACTATTG+AGG - chr1_3:64225538-64225557 Msa0132970:intron 30.0%
! CTTATTTCTTAAAGGTGACT+CGG - chr1_3:64225565-64225584 Msa0132970:intron 30.0%
! TTATTTCTTAAAGGTGACTC+GGG - chr1_3:64225566-64225585 Msa0132970:intron 30.0%
TAATTGAAAGTTGAAACTCC+TGG + chr1_3:64225646-64225665 None:intergenic 30.0%
TCATTGATAAATGGAAGGTA+GGG + chr1_3:64225670-64225689 None:intergenic 30.0%
TTCATTGATAAATGGAAGGT+AGG + chr1_3:64225671-64225690 None:intergenic 30.0%
!!! TCTTATTTTGACCATCAAAC+AGG + chr1_3:64225747-64225766 None:intergenic 30.0%
GAGACATTCATTCTTACAAA+TGG + chr1_3:64225929-64225948 None:intergenic 30.0%
! AAGGAATGGAATGATTTGTA+TGG - chr1_3:64226057-64226076 Msa0132970:CDS 30.0%
!! TGTGGAAGCCAATTTTAATA+CGG - chr1_3:64226131-64226150 Msa0132970:intron 30.0%
TGATGTGTCCGTATTAAAAT+TGG + chr1_3:64226142-64226161 None:intergenic 30.0%
ACACATCAATATTTGTCCAA+AGG - chr1_3:64226154-64226173 Msa0132970:intron 30.0%
GGCTTAAAATATACATCCTT+TGG + chr1_3:64226173-64226192 None:intergenic 30.0%
! ATATAGTGGAAGTGATCTTA+AGG - chr1_3:64224784-64224803 Msa0132970:three_prime_UTR 30.0%
TGTTGTATTAGTTCCTTAAG+GGG + chr1_3:64224918-64224937 None:intergenic 30.0%
CTGTTGTATTAGTTCCTTAA+GGG + chr1_3:64224919-64224938 None:intergenic 30.0%
TTTATCTTCTTCAGTAGCAA+TGG + chr1_3:64225093-64225112 None:intergenic 30.0%
GACAAACATCATTACTATTG+AGG - chr1_3:64225538-64225557 Msa0132970:intron 30.0%
! CTTATTTCTTAAAGGTGACT+CGG - chr1_3:64225565-64225584 Msa0132970:intron 30.0%
! TTATTTCTTAAAGGTGACTC+GGG - chr1_3:64225566-64225585 Msa0132970:intron 30.0%
TAATTGAAAGTTGAAACTCC+TGG + chr1_3:64225646-64225665 None:intergenic 30.0%
TCATTGATAAATGGAAGGTA+GGG + chr1_3:64225670-64225689 None:intergenic 30.0%
TTCATTGATAAATGGAAGGT+AGG + chr1_3:64225671-64225690 None:intergenic 30.0%
!!! TCTTATTTTGACCATCAAAC+AGG + chr1_3:64225747-64225766 None:intergenic 30.0%
GAGACATTCATTCTTACAAA+TGG + chr1_3:64225929-64225948 None:intergenic 30.0%
! AAGGAATGGAATGATTTGTA+TGG - chr1_3:64226057-64226076 Msa0132970:CDS 30.0%
!! TGTGGAAGCCAATTTTAATA+CGG - chr1_3:64226131-64226150 Msa0132970:intron 30.0%
TGATGTGTCCGTATTAAAAT+TGG + chr1_3:64226142-64226161 None:intergenic 30.0%
ACACATCAATATTTGTCCAA+AGG - chr1_3:64226154-64226173 Msa0132970:intron 30.0%
GGCTTAAAATATACATCCTT+TGG + chr1_3:64226173-64226192 None:intergenic 30.0%
ACTATGACAGAAGGATATAG+TGG - chr1_3:64224770-64224789 Msa0132970:three_prime_UTR 35.0%
!! ATTTCTAGTTCCCTACTTGT+TGG - chr1_3:64224829-64224848 Msa0132970:three_prime_UTR 35.0%
ATTATCACCACCAACAAGTA+GGG + chr1_3:64224842-64224861 None:intergenic 35.0%
GTTGTATTAGTTCCTTAAGG+GGG + chr1_3:64224917-64224936 None:intergenic 35.0%
CCTGTTGTATTAGTTCCTTA+AGG + chr1_3:64224920-64224939 None:intergenic 35.0%
CCTTAAGGAACTAATACAAC+AGG - chr1_3:64224917-64224936 Msa0132970:CDS 35.0%
ACAGGAGAAGGAAAAAGAAA+TGG - chr1_3:64224935-64224954 Msa0132970:CDS 35.0%
CTCATGTCTTCCATGTTTAA+TGG + chr1_3:64225140-64225159 None:intergenic 35.0%
! GCTTATTTGAGTTTATCGAC+TGG - chr1_3:64225236-64225255 Msa0132970:intron 35.0%
AATAGGCTGAAAACAGCTTA+TGG + chr1_3:64225315-64225334 None:intergenic 35.0%
CTTGAAGAGCTTATGGAAAT+AGG + chr1_3:64225332-64225351 None:intergenic 35.0%
AAGCTATCTTGAAGAGCTTA+TGG + chr1_3:64225339-64225358 None:intergenic 35.0%
! ATATTTTACCAGGCAGTACA+AGG + chr1_3:64225514-64225533 None:intergenic 35.0%
! GGGAATAGATATCTGGAATT+TGG - chr1_3:64225586-64225605 Msa0132970:intron 35.0%
GAGACAGGATATGAATATGA+TGG + chr1_3:64225783-64225802 None:intergenic 35.0%
TCTCTCTTATCAGATAGCAA+TGG + chr1_3:64225809-64225828 None:intergenic 35.0%
ATGGATGCATGTGTAAATAC+AGG - chr1_3:64225990-64226009 Msa0132970:intron 35.0%
! TGGAATGATTTGTATGGAGA+AGG - chr1_3:64226063-64226082 Msa0132970:CDS 35.0%
! AATGATTTGTATGGAGAAGG+AGG - chr1_3:64226066-64226085 Msa0132970:CDS 35.0%
ACTATGACAGAAGGATATAG+TGG - chr1_3:64224770-64224789 Msa0132970:three_prime_UTR 35.0%
!! ATTTCTAGTTCCCTACTTGT+TGG - chr1_3:64224829-64224848 Msa0132970:three_prime_UTR 35.0%
ATTATCACCACCAACAAGTA+GGG + chr1_3:64224842-64224861 None:intergenic 35.0%
GTTGTATTAGTTCCTTAAGG+GGG + chr1_3:64224917-64224936 None:intergenic 35.0%
CCTGTTGTATTAGTTCCTTA+AGG + chr1_3:64224920-64224939 None:intergenic 35.0%
CCTTAAGGAACTAATACAAC+AGG - chr1_3:64224917-64224936 Msa0132970:CDS 35.0%
ACAGGAGAAGGAAAAAGAAA+TGG - chr1_3:64224935-64224954 Msa0132970:CDS 35.0%
CTCATGTCTTCCATGTTTAA+TGG + chr1_3:64225140-64225159 None:intergenic 35.0%
! GCTTATTTGAGTTTATCGAC+TGG - chr1_3:64225236-64225255 Msa0132970:intron 35.0%
AATAGGCTGAAAACAGCTTA+TGG + chr1_3:64225315-64225334 None:intergenic 35.0%
CTTGAAGAGCTTATGGAAAT+AGG + chr1_3:64225332-64225351 None:intergenic 35.0%
AAGCTATCTTGAAGAGCTTA+TGG + chr1_3:64225339-64225358 None:intergenic 35.0%
! ATATTTTACCAGGCAGTACA+AGG + chr1_3:64225514-64225533 None:intergenic 35.0%
! GGGAATAGATATCTGGAATT+TGG - chr1_3:64225586-64225605 Msa0132970:intron 35.0%
GAGACAGGATATGAATATGA+TGG + chr1_3:64225783-64225802 None:intergenic 35.0%
TCTCTCTTATCAGATAGCAA+TGG + chr1_3:64225809-64225828 None:intergenic 35.0%
ATGGATGCATGTGTAAATAC+AGG - chr1_3:64225990-64226009 Msa0132970:intron 35.0%
! TGGAATGATTTGTATGGAGA+AGG - chr1_3:64226063-64226082 Msa0132970:CDS 35.0%
! AATGATTTGTATGGAGAAGG+AGG - chr1_3:64226066-64226085 Msa0132970:CDS 35.0%
! ATTTTGAGCAGGATCATGGT+TGG - chr1_3:64224659-64224678 Msa0132970:three_prime_UTR 40.0%
GAGCTTTCAACTATGACAGA+AGG - chr1_3:64224761-64224780 Msa0132970:three_prime_UTR 40.0%
GATTATCACCACCAACAAGT+AGG + chr1_3:64224843-64224862 None:intergenic 40.0%
GGAACTAATACAACAGGAGA+AGG - chr1_3:64224923-64224942 Msa0132970:CDS 40.0%
TTCCATGTTTAATGGCCTGA+GGG + chr1_3:64225132-64225151 None:intergenic 40.0%
AAACATGGAAGACATGAGAG+AGG - chr1_3:64225142-64225161 Msa0132970:intron 40.0%
CATGAGAGAGGCAAAGAATA+AGG - chr1_3:64225154-64225173 Msa0132970:intron 40.0%
CTACAGCAATTGAAGTGAAC+AGG + chr1_3:64225195-64225214 None:intergenic 40.0%
ACTTCAATTGCTGTAGAGCT+AGG - chr1_3:64225199-64225218 Msa0132970:intron 40.0%
CTTCAATTGCTGTAGAGCTA+GGG - chr1_3:64225200-64225219 Msa0132970:intron 40.0%
AAAGGCCTTAGCAAGTGATT+TGG - chr1_3:64225480-64225499 Msa0132970:intron 40.0%
AAGGCCTTAGCAAGTGATTT+GGG - chr1_3:64225481-64225500 Msa0132970:intron 40.0%
TTCCTATCTTGTGCTTCTCT+AGG - chr1_3:64225709-64225728 Msa0132970:intron 40.0%
! TTCCTAGAGAAGCACAAGAT+AGG + chr1_3:64225714-64225733 None:intergenic 40.0%
! TGCTTCTCTAGGAATCACTT+AGG - chr1_3:64225720-64225739 Msa0132970:intron 40.0%
! GCTTCTCTAGGAATCACTTA+GGG - chr1_3:64225721-64225740 Msa0132970:intron 40.0%
!! AGATAGCAATGGATTGAGAC+AGG + chr1_3:64225798-64225817 None:intergenic 40.0%
TCTTGCAAAAAAAAGAGGGC+TGG + chr1_3:64225839-64225858 None:intergenic 40.0%
GATGCATGTGTAAATACAGG+TGG - chr1_3:64225993-64226012 Msa0132970:intron 40.0%
! ATCAACAATGACCAGGTTGA+AGG - chr1_3:64226038-64226057 Msa0132970:CDS 40.0%
ATCATTCCATTCCTTCAACC+TGG + chr1_3:64226052-64226071 None:intergenic 40.0%
! ATTTTGAGCAGGATCATGGT+TGG - chr1_3:64224659-64224678 Msa0132970:three_prime_UTR 40.0%
GAGCTTTCAACTATGACAGA+AGG - chr1_3:64224761-64224780 Msa0132970:three_prime_UTR 40.0%
GATTATCACCACCAACAAGT+AGG + chr1_3:64224843-64224862 None:intergenic 40.0%
GGAACTAATACAACAGGAGA+AGG - chr1_3:64224923-64224942 Msa0132970:CDS 40.0%
TTCCATGTTTAATGGCCTGA+GGG + chr1_3:64225132-64225151 None:intergenic 40.0%
AAACATGGAAGACATGAGAG+AGG - chr1_3:64225142-64225161 Msa0132970:intron 40.0%
CATGAGAGAGGCAAAGAATA+AGG - chr1_3:64225154-64225173 Msa0132970:intron 40.0%
CTACAGCAATTGAAGTGAAC+AGG + chr1_3:64225195-64225214 None:intergenic 40.0%
ACTTCAATTGCTGTAGAGCT+AGG - chr1_3:64225199-64225218 Msa0132970:intron 40.0%
CTTCAATTGCTGTAGAGCTA+GGG - chr1_3:64225200-64225219 Msa0132970:intron 40.0%
AAAGGCCTTAGCAAGTGATT+TGG - chr1_3:64225480-64225499 Msa0132970:intron 40.0%
AAGGCCTTAGCAAGTGATTT+GGG - chr1_3:64225481-64225500 Msa0132970:intron 40.0%
TTCCTATCTTGTGCTTCTCT+AGG - chr1_3:64225709-64225728 Msa0132970:intron 40.0%
! TTCCTAGAGAAGCACAAGAT+AGG + chr1_3:64225714-64225733 None:intergenic 40.0%
! TGCTTCTCTAGGAATCACTT+AGG - chr1_3:64225720-64225739 Msa0132970:intron 40.0%
! GCTTCTCTAGGAATCACTTA+GGG - chr1_3:64225721-64225740 Msa0132970:intron 40.0%
!! AGATAGCAATGGATTGAGAC+AGG + chr1_3:64225798-64225817 None:intergenic 40.0%
TCTTGCAAAAAAAAGAGGGC+TGG + chr1_3:64225839-64225858 None:intergenic 40.0%
GATGCATGTGTAAATACAGG+TGG - chr1_3:64225993-64226012 Msa0132970:intron 40.0%
! ATCAACAATGACCAGGTTGA+AGG - chr1_3:64226038-64226057 Msa0132970:CDS 40.0%
ATCATTCCATTCCTTCAACC+TGG + chr1_3:64226052-64226071 None:intergenic 40.0%
! GGCAATTTTGAGCAGGATCA+TGG - chr1_3:64224655-64224674 Msa0132970:three_prime_UTR 45.0%
TCTTCCGTCTGCAGAAAACA+GGG - chr1_3:64224682-64224701 Msa0132970:three_prime_UTR 45.0%
! GTTTCCCTGTTTTCTGCAGA+CGG + chr1_3:64224689-64224708 None:intergenic 45.0%
! TCTAGTTCCCTACTTGTTGG+TGG - chr1_3:64224832-64224851 Msa0132970:three_prime_UTR 45.0%
AAGATCAAGTGATTCCCCTC+AGG - chr1_3:64225114-64225133 Msa0132970:intron 45.0%
TCCATGTTTAATGGCCTGAG+GGG + chr1_3:64225131-64225150 None:intergenic 45.0%
CTTCCATGTTTAATGGCCTG+AGG + chr1_3:64225133-64225152 None:intergenic 45.0%
AGGCAAAGAATAAGGTGCGT+TGG - chr1_3:64225162-64225181 Msa0132970:intron 45.0%
!! GAGCTAGGGCTTGTTTCTAT+TGG - chr1_3:64225214-64225233 Msa0132970:intron 45.0%
! CACTTGCTAAGGCCTTTGTA+TGG + chr1_3:64225477-64225496 None:intergenic 45.0%
! GTGACTCGGGAATAGATATC+TGG - chr1_3:64225579-64225598 Msa0132970:intron 45.0%
ATCACTTAGGGCCTGTTTGA+TGG - chr1_3:64225733-64225752 Msa0132970:intron 45.0%
CAGAAGGATCAACAATGACC+AGG - chr1_3:64226031-64226050 Msa0132970:intron 45.0%
! CAATGACCAGGTTGAAGGAA+TGG - chr1_3:64226043-64226062 Msa0132970:CDS 45.0%
TGTATGGAGAAGGAGGTTCA+AGG - chr1_3:64226073-64226092 Msa0132970:CDS 45.0%
AGGAGGTTCAAGGAAGAAAG+AGG - chr1_3:64226083-64226102 Msa0132970:intron 45.0%
! GGCAATTTTGAGCAGGATCA+TGG - chr1_3:64224655-64224674 Msa0132970:three_prime_UTR 45.0%
TCTTCCGTCTGCAGAAAACA+GGG - chr1_3:64224682-64224701 Msa0132970:three_prime_UTR 45.0%
! GTTTCCCTGTTTTCTGCAGA+CGG + chr1_3:64224689-64224708 None:intergenic 45.0%
! TCTAGTTCCCTACTTGTTGG+TGG - chr1_3:64224832-64224851 Msa0132970:three_prime_UTR 45.0%
AAGATCAAGTGATTCCCCTC+AGG - chr1_3:64225114-64225133 Msa0132970:intron 45.0%
TCCATGTTTAATGGCCTGAG+GGG + chr1_3:64225131-64225150 None:intergenic 45.0%
CTTCCATGTTTAATGGCCTG+AGG + chr1_3:64225133-64225152 None:intergenic 45.0%
AGGCAAAGAATAAGGTGCGT+TGG - chr1_3:64225162-64225181 Msa0132970:intron 45.0%
!! GAGCTAGGGCTTGTTTCTAT+TGG - chr1_3:64225214-64225233 Msa0132970:intron 45.0%
! CACTTGCTAAGGCCTTTGTA+TGG + chr1_3:64225477-64225496 None:intergenic 45.0%
! GTGACTCGGGAATAGATATC+TGG - chr1_3:64225579-64225598 Msa0132970:intron 45.0%
ATCACTTAGGGCCTGTTTGA+TGG - chr1_3:64225733-64225752 Msa0132970:intron 45.0%
CAGAAGGATCAACAATGACC+AGG - chr1_3:64226031-64226050 Msa0132970:intron 45.0%
! CAATGACCAGGTTGAAGGAA+TGG - chr1_3:64226043-64226062 Msa0132970:CDS 45.0%
TGTATGGAGAAGGAGGTTCA+AGG - chr1_3:64226073-64226092 Msa0132970:CDS 45.0%
AGGAGGTTCAAGGAAGAAAG+AGG - chr1_3:64226083-64226102 Msa0132970:intron 45.0%
GTCTTCCGTCTGCAGAAAAC+AGG - chr1_3:64224681-64224700 Msa0132970:three_prime_UTR 50.0%
GATATGCTGCAGTCATGCAC+AGG + chr1_3:64224893-64224912 None:intergenic 50.0%
TCCCCTCAGGCCATTAAACA+TGG - chr1_3:64225127-64225146 Msa0132970:intron 50.0%
! GGCACCCAAATCACTTGCTA+AGG + chr1_3:64225488-64225507 None:intergenic 50.0%
TTACCAGGCAGTACAAGGCA+AGG + chr1_3:64225509-64225528 None:intergenic 50.0%
! GCTGCAAGTTTTGCTGCAGA+AGG - chr1_3:64226015-64226034 Msa0132970:intron 50.0%
GTCTTCCGTCTGCAGAAAAC+AGG - chr1_3:64224681-64224700 Msa0132970:three_prime_UTR 50.0%
GATATGCTGCAGTCATGCAC+AGG + chr1_3:64224893-64224912 None:intergenic 50.0%
TCCCCTCAGGCCATTAAACA+TGG - chr1_3:64225127-64225146 Msa0132970:intron 50.0%
! GGCACCCAAATCACTTGCTA+AGG + chr1_3:64225488-64225507 None:intergenic 50.0%
TTACCAGGCAGTACAAGGCA+AGG + chr1_3:64225509-64225528 None:intergenic 50.0%
! GCTGCAAGTTTTGCTGCAGA+AGG - chr1_3:64226015-64226034 Msa0132970:intron 50.0%
TGCAGCATATCGCCCCCTTA+AGG - chr1_3:64224902-64224921 Msa0132970:CDS 55.0%
TTCGGCTAAACAGACCCGTG+CGG + chr1_3:64226270-64226289 None:intergenic 55.0%
TGCAGCATATCGCCCCCTTA+AGG - chr1_3:64224902-64224921 Msa0132970:CDS 55.0%
TTCGGCTAAACAGACCCGTG+CGG + chr1_3:64226270-64226289 None:intergenic 55.0%
GTGCCTTGCCTTGTACTGCC+TGG - chr1_3:64225503-64225522 Msa0132970:intron 60.0%
GTGCCTTGCCTTGTACTGCC+TGG - chr1_3:64225503-64225522 Msa0132970:intron 60.0%
TTGTTGACCCGTGCGCCGCA+CGG - chr1_3:64226252-64226271 Msa0132970:CDS 65.0%
AAACAGACCCGTGCGGCGCA+CGG + chr1_3:64226263-64226282 None:intergenic 65.0%
TTGTTGACCCGTGCGCCGCA+CGG - chr1_3:64226252-64226271 Msa0132970:CDS 65.0%
AAACAGACCCGTGCGGCGCA+CGG + chr1_3:64226263-64226282 None:intergenic 65.0%
TGTTGACCCGTGCGCCGCAC+GGG - chr1_3:64226253-64226272 Msa0132970:CDS 70.0%
AACAGACCCGTGCGGCGCAC+GGG + chr1_3:64226262-64226281 None:intergenic 70.0%
TGTTGACCCGTGCGCCGCAC+GGG - chr1_3:64226253-64226272 Msa0132970:CDS 70.0%
AACAGACCCGTGCGGCGCAC+GGG + chr1_3:64226262-64226281 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 64224653 64226335 64224653 ID=Msa0132970;Name=Msa0132970
chr1_3 mRNA 64224653 64226335 64224653 ID=Msa0132970-mRNA-1;Parent=Msa0132970;Name=Msa0132970-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0.66|0.5|1|1|1|4|207|138
chr1_3 exon 64224653 64224976 64224653 ID=Msa0132970-mRNA-1:exon:21594;Parent=Msa0132970-mRNA-1
chr1_3 exon 64225867 64225944 64225867 ID=Msa0132970-mRNA-1:exon:21593;Parent=Msa0132970-mRNA-1
chr1_3 exon 64226032 64226100 64226032 ID=Msa0132970-mRNA-1:exon:21592;Parent=Msa0132970-mRNA-1
chr1_3 exon 64226183 64226335 64226183 ID=Msa0132970-mRNA-1:exon:21591;Parent=Msa0132970-mRNA-1
chr1_3 CDS 64226183 64226335 64226183 ID=Msa0132970-mRNA-1:cds;Parent=Msa0132970-mRNA-1
chr1_3 CDS 64226032 64226100 64226032 ID=Msa0132970-mRNA-1:cds;Parent=Msa0132970-mRNA-1
chr1_3 CDS 64225867 64225944 64225867 ID=Msa0132970-mRNA-1:cds;Parent=Msa0132970-mRNA-1
chr1_3 CDS 64224860 64224976 64224860 ID=Msa0132970-mRNA-1:cds;Parent=Msa0132970-mRNA-1
chr1_3 three_prime_UTR 64224653 64224859 64224653 ID=Msa0132970-mRNA-1:three_prime_utr;Parent=Msa0132970-mRNA-1
Gene Sequence

>Msa0132970

ATGGCAATTTTGAGCAGGATCATGGTTGGTCTTCCGTCTGCAGAAAACAGGGAAACAATCTTGAAAACTATTCTAGCTAATGAAAAATCTGAAAATATGAACTTCGAAGAGCTTTCAACTATGACAGAAGGATATAGTGGAAGTGATCTTAAGAACCTGTGCATGACTGCAGCATATCGCCCCCTTAAGGAACTAATACAACAGGAGAAGGAAAAAGAAATGAAAAAGAAGAAAAAGGTGACAGAAGTTGAAATCTCAGAAGACGCTTCCATTGCTACTGAAGAAGATAAAGAAGATCAAGTGGCTGCAAGTTTTGCTGCAGAAGGATCAACAATGACCAGGTTGAAGGAATGGAATGATTTGTATGGAGAAGGAGGTTCAAGGAAGAAAGAGGAGCAGCTCAGTTATTTCTTTTAG
Protein sequence

>Msa0132970

MAILSRIMVGLPSAENRETILKTILANEKSENMNFEELSTMTEGYSGSDLKNLCMTAAYRPLKELIQQEKEKEMKKKKKVTEVEISEDASIATEEDKEDQVAASFAAEGSTMTRLKEWNDLYGEGGSRKKEEQLSYFF*