AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0133310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0133310 MtrunA17_Chr4g0033781 96.212 132 5 0 1 132 277 408 3.95e-90 267
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0133310 AT2G46200.1 59.756 82 30 1 54 132 290 371 6.07e-29 108
Msa0133310 AT2G46200.2 59.756 82 30 1 54 132 290 371 6.07e-29 108
Msa0133310 AT2G46200.4 48.387 62 29 1 54 112 290 351 3.61e-13 65.1

Find 53 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGGTTCTGCATGGCTTTAC+TGG 0.167077 1_3:+64579552 None:intergenic
CTCCTCCAGCCAACGAGATA+TGG 0.241122 1_3:-64578865 Msa0133310:CDS
ATAGAACTCAATCGGTAAAT+TGG 0.263314 1_3:+64579366 None:intergenic
GGAAAGAAGGAAAATACTTT+TGG 0.280650 1_3:-64578613 Msa0133310:three_prime_UTR
ATGCAGAAATGATAGTGATT+TGG 0.281057 1_3:-64578507 Msa0133310:three_prime_UTR
CGAGATATGGGCATCTTAAT+TGG 0.290801 1_3:-64578852 Msa0133310:exon
CCTTCCTCCCGGTCGAATAT+AGG 0.327458 1_3:+64579044 None:intergenic
TGCAGAAATGATAGTGATTT+GGG 0.336866 1_3:-64578506 Msa0133310:three_prime_UTR
CAATGATGAAAGTCAGATTA+AGG 0.364871 1_3:-64579509 Msa0133310:CDS
TTATCACTGTTGAATGTACT+TGG 0.379549 1_3:-64578634 Msa0133310:three_prime_UTR
AGCTTACACCGGGGCTTGCC+AGG 0.388675 1_3:+64578743 None:intergenic
ATCCCTCTTCGGCTACACTA+TGG 0.392785 1_3:+64579467 None:intergenic
TGGAGGAGCTTATGATCCTT+TGG 0.396538 1_3:-64579389 Msa0133310:CDS
ATCTCGTTGGCTGGAGGAGG+AGG 0.421763 1_3:+64578869 None:intergenic
GCATCCGAAGGACAGAACTA+TGG 0.438386 1_3:-64579409 Msa0133310:CDS
GTCAGATTAAGGAGAACAAC+AGG 0.444589 1_3:-64579498 Msa0133310:CDS
TCCTCCATAGTTCTGTCCTT+CGG 0.451599 1_3:+64579405 None:intergenic
GAGTTCTATCATTATTATCA+TGG 0.459001 1_3:-64579352 Msa0133310:CDS
TCCTCCAGCCAACGAGATAT+GGG 0.464743 1_3:-64578864 Msa0133310:CDS
GTTGAATGTACTTGGAAAGA+AGG 0.483405 1_3:-64578626 Msa0133310:three_prime_UTR
CATGGCTTTACTGGATTCCT+CGG 0.484046 1_3:+64579561 None:intergenic
AATCTCATGCAGGTTAGAAT+AGG 0.491283 1_3:-64579075 Msa0133310:intron
GATGCCCATATCTCGTTGGC+TGG 0.497660 1_3:+64578860 None:intergenic
CATATCTCGTTGGCTGGAGG+AGG 0.504973 1_3:+64578866 None:intergenic
TAATAATGATAGAACTCAAT+CGG 0.507283 1_3:+64579358 None:intergenic
AGTTGCATCTAATCCCTCTT+CGG 0.512994 1_3:+64579456 None:intergenic
ATGATATATTTATGCAATGC+TGG 0.518777 1_3:+64578796 None:intergenic
TTTCTTTCGAAAGTTTCCCT+CGG 0.520168 1_3:+64579533 None:intergenic
GGAGGCTGAACCCAGTGTCC+TGG 0.524624 1_3:+64578887 None:intergenic
CCTATATTCGACCGGGAGGA+AGG 0.539475 1_3:-64579044 Msa0133310:intron
ATGGGATGCCTATATTCGAC+CGG 0.541340 1_3:-64579052 Msa0133310:CDS
AAGTTTCCCTCGGTTCTGCA+TGG 0.542418 1_3:+64579543 None:intergenic
TCATGCAGGTTAGAATAGGA+TGG 0.550259 1_3:-64579071 Msa0133310:intron
ACTTTGTGCAGTCGCATACC+AGG 0.571448 1_3:-64578905 Msa0133310:intron
CAGTCGCATACCAGGACACT+GGG 0.574072 1_3:-64578897 Msa0133310:CDS
TCATGGAAGCAACAATGACA+TGG 0.575257 1_3:-64579335 Msa0133310:CDS
CATGCAGGTTAGAATAGGAT+GGG 0.585291 1_3:-64579070 Msa0133310:intron
GTCCATAGTGTAGCCGAAGA+GGG 0.591775 1_3:-64579469 Msa0133310:CDS
GCCCATATCTCGTTGGCTGG+AGG 0.613148 1_3:+64578863 None:intergenic
AGTCCATAGTGTAGCCGAAG+AGG 0.619578 1_3:-64579470 Msa0133310:CDS
AGATGCAATTGCAACTGCCG+AGG 0.619618 1_3:-64579578 Msa0133310:CDS
TTAAGATGCCCATATCTCGT+TGG 0.621646 1_3:+64578856 None:intergenic
GCAGTCGCATACCAGGACAC+TGG 0.624972 1_3:-64578898 Msa0133310:intron
GTAAAGCCATGCAGAACCGA+GGG 0.633620 1_3:-64579549 Msa0133310:CDS
AGTAAAGCCATGCAGAACCG+AGG 0.640787 1_3:-64579550 Msa0133310:CDS
TGGGATGCCTATATTCGACC+GGG 0.643337 1_3:-64579051 Msa0133310:CDS
TATGGGCATCTTAATTGGTG+AGG 0.644535 1_3:-64578847 Msa0133310:exon
TCTAGTAACATAGCATCCGA+AGG 0.647457 1_3:-64579421 Msa0133310:CDS
TCAATCGGTAAATTGGCCAA+AGG 0.652661 1_3:+64579373 None:intergenic
TCCGAAGGACAGAACTATGG+AGG 0.665419 1_3:-64579406 Msa0133310:CDS
CATGGAAGCAACAATGACAT+GGG 0.669121 1_3:-64579334 Msa0133310:CDS
TGACATGGGCACATTGATCG+AGG 0.675577 1_3:-64579320 Msa0133310:intron
GATGCCTATATTCGACCGGG+AGG 0.757591 1_3:-64579048 Msa0133310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATAATAATAATAAAAAAAG+TGG + chr1_3:64578964-64578983 None:intergenic 10.0%
!!! CACTTTTTTTATTATTATTA+TGG - chr1_3:64578962-64578981 Msa0133310:intron 10.0%
!! CATAATAATAATAAAAAAAG+TGG + chr1_3:64578964-64578983 None:intergenic 10.0%
!!! CACTTTTTTTATTATTATTA+TGG - chr1_3:64578962-64578981 Msa0133310:intron 10.0%
!! TAATAATGATAGAACTCAAT+CGG + chr1_3:64578746-64578765 None:intergenic 20.0%
!!! TGTTTCTTTAGAATTCATAT+TGG - chr1_3:64578865-64578884 Msa0133310:CDS 20.0%
!! TAATAATGATAGAACTCAAT+CGG + chr1_3:64578746-64578765 None:intergenic 20.0%
!!! TGTTTCTTTAGAATTCATAT+TGG - chr1_3:64578865-64578884 Msa0133310:CDS 20.0%
! GAGTTCTATCATTATTATCA+TGG - chr1_3:64578749-64578768 Msa0133310:three_prime_UTR 25.0%
!!! TTCTTTAGAATTCATATTGG+TGG - chr1_3:64578868-64578887 Msa0133310:CDS 25.0%
! ATGATATATTTATGCAATGC+TGG + chr1_3:64579308-64579327 None:intergenic 25.0%
!!! TATCATCTGATATGTTTTGA+AGG - chr1_3:64579322-64579341 Msa0133310:CDS 25.0%
!!! ATATGTTTTGAAGGTTTGAA+CGG - chr1_3:64579331-64579350 Msa0133310:CDS 25.0%
! AAAAAAATGATGATTAGCAC+TGG + chr1_3:64579437-64579456 None:intergenic 25.0%
!!! GTGCTAATCATCATTTTTTT+AGG - chr1_3:64579437-64579456 Msa0133310:CDS 25.0%
! GAGTTCTATCATTATTATCA+TGG - chr1_3:64578749-64578768 Msa0133310:three_prime_UTR 25.0%
!!! TTCTTTAGAATTCATATTGG+TGG - chr1_3:64578868-64578887 Msa0133310:CDS 25.0%
! ATGATATATTTATGCAATGC+TGG + chr1_3:64579308-64579327 None:intergenic 25.0%
!!! TATCATCTGATATGTTTTGA+AGG - chr1_3:64579322-64579341 Msa0133310:CDS 25.0%
!!! ATATGTTTTGAAGGTTTGAA+CGG - chr1_3:64579331-64579350 Msa0133310:CDS 25.0%
! AAAAAAATGATGATTAGCAC+TGG + chr1_3:64579437-64579456 None:intergenic 25.0%
!!! GTGCTAATCATCATTTTTTT+AGG - chr1_3:64579437-64579456 Msa0133310:CDS 25.0%
CAATGATGAAAGTCAGATTA+AGG - chr1_3:64578592-64578611 Msa0133310:three_prime_UTR 30.0%
ATAGAACTCAATCGGTAAAT+TGG + chr1_3:64578738-64578757 None:intergenic 30.0%
!!! TTAATTTTTTACCTTCCTCC+CGG + chr1_3:64579071-64579090 None:intergenic 30.0%
TTATCACTGTTGAATGTACT+TGG - chr1_3:64579467-64579486 Msa0133310:CDS 30.0%
! GGAAAGAAGGAAAATACTTT+TGG - chr1_3:64579488-64579507 Msa0133310:CDS 30.0%
! ATGCAGAAATGATAGTGATT+TGG - chr1_3:64579594-64579613 Msa0133310:CDS 30.0%
! TGCAGAAATGATAGTGATTT+GGG - chr1_3:64579595-64579614 Msa0133310:CDS 30.0%
CAATGATGAAAGTCAGATTA+AGG - chr1_3:64578592-64578611 Msa0133310:three_prime_UTR 30.0%
ATAGAACTCAATCGGTAAAT+TGG + chr1_3:64578738-64578757 None:intergenic 30.0%
!!! TTAATTTTTTACCTTCCTCC+CGG + chr1_3:64579071-64579090 None:intergenic 30.0%
TTATCACTGTTGAATGTACT+TGG - chr1_3:64579467-64579486 Msa0133310:CDS 30.0%
! GGAAAGAAGGAAAATACTTT+TGG - chr1_3:64579488-64579507 Msa0133310:CDS 30.0%
! ATGCAGAAATGATAGTGATT+TGG - chr1_3:64579594-64579613 Msa0133310:CDS 30.0%
TTTCTTTCGAAAGTTTCCCT+CGG + chr1_3:64578571-64578590 None:intergenic 35.0%
TGAGATTATTCAGCAGTCAA+TGG + chr1_3:64579013-64579032 None:intergenic 35.0%
AATCTCATGCAGGTTAGAAT+AGG - chr1_3:64579026-64579045 Msa0133310:intron 35.0%
! CACTGGAATTTTACACAGAA+TGG + chr1_3:64579420-64579439 None:intergenic 35.0%
GTTGAATGTACTTGGAAAGA+AGG - chr1_3:64579475-64579494 Msa0133310:CDS 35.0%
! GCAGAAATGATAGTGATTTG+GGG - chr1_3:64579596-64579615 Msa0133310:CDS 35.0%
TTTCTTTCGAAAGTTTCCCT+CGG + chr1_3:64578571-64578590 None:intergenic 35.0%
TGAGATTATTCAGCAGTCAA+TGG + chr1_3:64579013-64579032 None:intergenic 35.0%
AATCTCATGCAGGTTAGAAT+AGG - chr1_3:64579026-64579045 Msa0133310:intron 35.0%
! CACTGGAATTTTACACAGAA+TGG + chr1_3:64579420-64579439 None:intergenic 35.0%
GTTGAATGTACTTGGAAAGA+AGG - chr1_3:64579475-64579494 Msa0133310:CDS 35.0%
GTCAGATTAAGGAGAACAAC+AGG - chr1_3:64578603-64578622 Msa0133310:three_prime_UTR 40.0%
AGTTGCATCTAATCCCTCTT+CGG + chr1_3:64578648-64578667 None:intergenic 40.0%
TCTAGTAACATAGCATCCGA+AGG - chr1_3:64578680-64578699 Msa0133310:three_prime_UTR 40.0%
TCAATCGGTAAATTGGCCAA+AGG + chr1_3:64578731-64578750 None:intergenic 40.0%
TCATGGAAGCAACAATGACA+TGG - chr1_3:64578766-64578785 Msa0133310:three_prime_UTR 40.0%
CATGGAAGCAACAATGACAT+GGG - chr1_3:64578767-64578786 Msa0133310:three_prime_UTR 40.0%
! ATTCAGCAGTCAATGGACTT+AGG + chr1_3:64579006-64579025 None:intergenic 40.0%
ACTGCTGAATAATCTCATGC+AGG - chr1_3:64579016-64579035 Msa0133310:intron 40.0%
TCATGCAGGTTAGAATAGGA+TGG - chr1_3:64579030-64579049 Msa0133310:intron 40.0%
CATGCAGGTTAGAATAGGAT+GGG - chr1_3:64579031-64579050 Msa0133310:intron 40.0%
TTAAGATGCCCATATCTCGT+TGG + chr1_3:64579248-64579267 None:intergenic 40.0%
CGAGATATGGGCATCTTAAT+TGG - chr1_3:64579249-64579268 Msa0133310:intron 40.0%
TATGGGCATCTTAATTGGTG+AGG - chr1_3:64579254-64579273 Msa0133310:intron 40.0%
GTCAGATTAAGGAGAACAAC+AGG - chr1_3:64578603-64578622 Msa0133310:three_prime_UTR 40.0%
AGTTGCATCTAATCCCTCTT+CGG + chr1_3:64578648-64578667 None:intergenic 40.0%
TCTAGTAACATAGCATCCGA+AGG - chr1_3:64578680-64578699 Msa0133310:three_prime_UTR 40.0%
TCAATCGGTAAATTGGCCAA+AGG + chr1_3:64578731-64578750 None:intergenic 40.0%
TCATGGAAGCAACAATGACA+TGG - chr1_3:64578766-64578785 Msa0133310:three_prime_UTR 40.0%
CATGGAAGCAACAATGACAT+GGG - chr1_3:64578767-64578786 Msa0133310:three_prime_UTR 40.0%
! ATTCAGCAGTCAATGGACTT+AGG + chr1_3:64579006-64579025 None:intergenic 40.0%
ACTGCTGAATAATCTCATGC+AGG - chr1_3:64579016-64579035 Msa0133310:intron 40.0%
TCATGCAGGTTAGAATAGGA+TGG - chr1_3:64579030-64579049 Msa0133310:intron 40.0%
CATGCAGGTTAGAATAGGAT+GGG - chr1_3:64579031-64579050 Msa0133310:intron 40.0%
TTAAGATGCCCATATCTCGT+TGG + chr1_3:64579248-64579267 None:intergenic 40.0%
CGAGATATGGGCATCTTAAT+TGG - chr1_3:64579249-64579268 Msa0133310:intron 40.0%
TATGGGCATCTTAATTGGTG+AGG - chr1_3:64579254-64579273 Msa0133310:intron 40.0%
! CATGGCTTTACTGGATTCCT+CGG + chr1_3:64578543-64578562 None:intergenic 45.0%
! TCCTCCATAGTTCTGTCCTT+CGG + chr1_3:64578699-64578718 None:intergenic 45.0%
TGGAGGAGCTTATGATCCTT+TGG - chr1_3:64578712-64578731 Msa0133310:three_prime_UTR 45.0%
ATGGGATGCCTATATTCGAC+CGG - chr1_3:64579049-64579068 Msa0133310:CDS 45.0%
GCACAAAGTTGCAAGGAACA+TGG + chr1_3:64579185-64579204 None:intergenic 45.0%
! GAAGGTTTGAACGGTTTTCC+TGG - chr1_3:64579340-64579359 Msa0133310:CDS 45.0%
TGCTGCCAAAAAGCTTACAC+CGG + chr1_3:64579372-64579391 None:intergenic 45.0%
! CATGGCTTTACTGGATTCCT+CGG + chr1_3:64578543-64578562 None:intergenic 45.0%
! TCCTCCATAGTTCTGTCCTT+CGG + chr1_3:64578699-64578718 None:intergenic 45.0%
TGGAGGAGCTTATGATCCTT+TGG - chr1_3:64578712-64578731 Msa0133310:three_prime_UTR 45.0%
ATGGGATGCCTATATTCGAC+CGG - chr1_3:64579049-64579068 Msa0133310:CDS 45.0%
GCACAAAGTTGCAAGGAACA+TGG + chr1_3:64579185-64579204 None:intergenic 45.0%
! GAAGGTTTGAACGGTTTTCC+TGG - chr1_3:64579340-64579359 Msa0133310:CDS 45.0%
TGCTGCCAAAAAGCTTACAC+CGG + chr1_3:64579372-64579391 None:intergenic 45.0%
!!! ACTTTTTTTATTATTATTAT+GGG - chr1_3:64578963-64578982 Msa0133310:intron 5.0%
!!! ACTTTTTTTATTATTATTAT+GGG - chr1_3:64578963-64578982 Msa0133310:intron 5.0%
AGATGCAATTGCAACTGCCG+AGG - chr1_3:64578523-64578542 Msa0133310:three_prime_UTR 50.0%
! TCGGTTCTGCATGGCTTTAC+TGG + chr1_3:64578552-64578571 None:intergenic 50.0%
AGTAAAGCCATGCAGAACCG+AGG - chr1_3:64578551-64578570 Msa0133310:three_prime_UTR 50.0%
GTAAAGCCATGCAGAACCGA+GGG - chr1_3:64578552-64578571 Msa0133310:three_prime_UTR 50.0%
AAGTTTCCCTCGGTTCTGCA+TGG + chr1_3:64578561-64578580 None:intergenic 50.0%
AGTCCATAGTGTAGCCGAAG+AGG - chr1_3:64578631-64578650 Msa0133310:three_prime_UTR 50.0%
GTCCATAGTGTAGCCGAAGA+GGG - chr1_3:64578632-64578651 Msa0133310:three_prime_UTR 50.0%
ATCCCTCTTCGGCTACACTA+TGG + chr1_3:64578637-64578656 None:intergenic 50.0%
GCATCCGAAGGACAGAACTA+TGG - chr1_3:64578692-64578711 Msa0133310:three_prime_UTR 50.0%
TCCGAAGGACAGAACTATGG+AGG - chr1_3:64578695-64578714 Msa0133310:three_prime_UTR 50.0%
TGACATGGGCACATTGATCG+AGG - chr1_3:64578781-64578800 Msa0133310:three_prime_UTR 50.0%
TGGGATGCCTATATTCGACC+GGG - chr1_3:64579050-64579069 Msa0133310:CDS 50.0%
TGCGACTGCACAAAGTTGCA+AGG + chr1_3:64579192-64579211 None:intergenic 50.0%
ACTTTGTGCAGTCGCATACC+AGG - chr1_3:64579196-64579215 Msa0133310:intron 50.0%
TCCTCCAGCCAACGAGATAT+GGG - chr1_3:64579237-64579256 Msa0133310:intron 50.0%
! AAGCCCCGGTGTAAGCTTTT+TGG - chr1_3:64579364-64579383 Msa0133310:CDS 50.0%
CTGCCAAAAAGCTTACACCG+GGG + chr1_3:64579370-64579389 None:intergenic 50.0%
GCTGCCAAAAAGCTTACACC+GGG + chr1_3:64579371-64579390 None:intergenic 50.0%
AGATGCAATTGCAACTGCCG+AGG - chr1_3:64578523-64578542 Msa0133310:three_prime_UTR 50.0%
! TCGGTTCTGCATGGCTTTAC+TGG + chr1_3:64578552-64578571 None:intergenic 50.0%
AGTAAAGCCATGCAGAACCG+AGG - chr1_3:64578551-64578570 Msa0133310:three_prime_UTR 50.0%
GTAAAGCCATGCAGAACCGA+GGG - chr1_3:64578552-64578571 Msa0133310:three_prime_UTR 50.0%
AAGTTTCCCTCGGTTCTGCA+TGG + chr1_3:64578561-64578580 None:intergenic 50.0%
AGTCCATAGTGTAGCCGAAG+AGG - chr1_3:64578631-64578650 Msa0133310:three_prime_UTR 50.0%
GTCCATAGTGTAGCCGAAGA+GGG - chr1_3:64578632-64578651 Msa0133310:three_prime_UTR 50.0%
ATCCCTCTTCGGCTACACTA+TGG + chr1_3:64578637-64578656 None:intergenic 50.0%
GCATCCGAAGGACAGAACTA+TGG - chr1_3:64578692-64578711 Msa0133310:three_prime_UTR 50.0%
TCCGAAGGACAGAACTATGG+AGG - chr1_3:64578695-64578714 Msa0133310:three_prime_UTR 50.0%
TGACATGGGCACATTGATCG+AGG - chr1_3:64578781-64578800 Msa0133310:three_prime_UTR 50.0%
TGGGATGCCTATATTCGACC+GGG - chr1_3:64579050-64579069 Msa0133310:CDS 50.0%
TGCGACTGCACAAAGTTGCA+AGG + chr1_3:64579192-64579211 None:intergenic 50.0%
ACTTTGTGCAGTCGCATACC+AGG - chr1_3:64579196-64579215 Msa0133310:intron 50.0%
TCCTCCAGCCAACGAGATAT+GGG - chr1_3:64579237-64579256 Msa0133310:intron 50.0%
! AAGCCCCGGTGTAAGCTTTT+TGG - chr1_3:64579364-64579383 Msa0133310:CDS 50.0%
CTGCCAAAAAGCTTACACCG+GGG + chr1_3:64579370-64579389 None:intergenic 50.0%
GCTGCCAAAAAGCTTACACC+GGG + chr1_3:64579371-64579390 None:intergenic 50.0%
TGGCACGTAACTGTGCACGA+AGG - chr1_3:64578888-64578907 Msa0133310:CDS 55.0%
GATGCCTATATTCGACCGGG+AGG - chr1_3:64579053-64579072 Msa0133310:CDS 55.0%
CCTTCCTCCCGGTCGAATAT+AGG + chr1_3:64579060-64579079 None:intergenic 55.0%
CCTATATTCGACCGGGAGGA+AGG - chr1_3:64579057-64579076 Msa0133310:CDS 55.0%
CAGTCGCATACCAGGACACT+GGG - chr1_3:64579204-64579223 Msa0133310:intron 55.0%
! CATATCTCGTTGGCTGGAGG+AGG + chr1_3:64579238-64579257 None:intergenic 55.0%
CTCCTCCAGCCAACGAGATA+TGG - chr1_3:64579236-64579255 Msa0133310:intron 55.0%
GATGCCCATATCTCGTTGGC+TGG + chr1_3:64579244-64579263 None:intergenic 55.0%
TGGCACGTAACTGTGCACGA+AGG - chr1_3:64578888-64578907 Msa0133310:CDS 55.0%
GATGCCTATATTCGACCGGG+AGG - chr1_3:64579053-64579072 Msa0133310:CDS 55.0%
CCTTCCTCCCGGTCGAATAT+AGG + chr1_3:64579060-64579079 None:intergenic 55.0%
CCTATATTCGACCGGGAGGA+AGG - chr1_3:64579057-64579076 Msa0133310:CDS 55.0%
CAGTCGCATACCAGGACACT+GGG - chr1_3:64579204-64579223 Msa0133310:intron 55.0%
! CATATCTCGTTGGCTGGAGG+AGG + chr1_3:64579238-64579257 None:intergenic 55.0%
CTCCTCCAGCCAACGAGATA+TGG - chr1_3:64579236-64579255 Msa0133310:intron 55.0%
GATGCCCATATCTCGTTGGC+TGG + chr1_3:64579244-64579263 None:intergenic 55.0%
GCAGTCGCATACCAGGACAC+TGG - chr1_3:64579203-64579222 Msa0133310:intron 60.0%
! ATCTCGTTGGCTGGAGGAGG+AGG + chr1_3:64579235-64579254 None:intergenic 60.0%
! GCCCATATCTCGTTGGCTGG+AGG + chr1_3:64579241-64579260 None:intergenic 60.0%
! ACGGTTTTCCTGGCAAGCCC+CGG - chr1_3:64579350-64579369 Msa0133310:CDS 60.0%
GCAGTCGCATACCAGGACAC+TGG - chr1_3:64579203-64579222 Msa0133310:intron 60.0%
! ATCTCGTTGGCTGGAGGAGG+AGG + chr1_3:64579235-64579254 None:intergenic 60.0%
! GCCCATATCTCGTTGGCTGG+AGG + chr1_3:64579241-64579260 None:intergenic 60.0%
! ACGGTTTTCCTGGCAAGCCC+CGG - chr1_3:64579350-64579369 Msa0133310:CDS 60.0%
GGAGGCTGAACCCAGTGTCC+TGG + chr1_3:64579217-64579236 None:intergenic 65.0%
AGCTTACACCGGGGCTTGCC+AGG + chr1_3:64579361-64579380 None:intergenic 65.0%
GGAGGCTGAACCCAGTGTCC+TGG + chr1_3:64579217-64579236 None:intergenic 65.0%
AGCTTACACCGGGGCTTGCC+AGG + chr1_3:64579361-64579380 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 64578506 64579617 64578506 ID=Msa0133310;Name=Msa0133310
chr1_3 mRNA 64578506 64579617 64578506 ID=Msa0133310-mRNA-1;Parent=Msa0133310;Name=Msa0133310-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|1|1|1|1|1|3|350|132
chr1_3 exon 64578506 64578916 64578506 ID=Msa0133310-mRNA-1:exon:21741;Parent=Msa0133310-mRNA-1
chr1_3 exon 64579045 64579085 64579045 ID=Msa0133310-mRNA-1:exon:21740;Parent=Msa0133310-mRNA-1
chr1_3 exon 64579321 64579617 64579321 ID=Msa0133310-mRNA-1:exon:21739;Parent=Msa0133310-mRNA-1
chr1_3 CDS 64579321 64579617 64579321 ID=Msa0133310-mRNA-1:cds;Parent=Msa0133310-mRNA-1
chr1_3 CDS 64579045 64579085 64579045 ID=Msa0133310-mRNA-1:cds;Parent=Msa0133310-mRNA-1
chr1_3 CDS 64578856 64578916 64578856 ID=Msa0133310-mRNA-1:cds;Parent=Msa0133310-mRNA-1
chr1_3 three_prime_UTR 64578506 64578855 64578506 ID=Msa0133310-mRNA-1:three_prime_utr;Parent=Msa0133310-mRNA-1
Gene Sequence

>Msa0133310

GAAACAGATGCTGCTAAAGATGCAATTGCAACTGCCGAGGAATCCAGTAAAGCCATGCAGAACCGAGGGAAACTTTCGAAAGAAAGCAATGATGAAAGTCAGATTAAGGAGAACAACAGGCAAATAGTCCATAGTGTAGCCGAAGAGGGATTAGATGCAACTGATGAGAAGAAATCTAGTAACATAGCATCCGAAGGACAGAACTATGGAGGAGCTTATGATCCTTTGGCCAATTTACCGATTGAGTTCTATCATTATTATCATGGAAGCAACAATGACATGGGCACATTGATCGAGGTTAGAATAGGATGGGATGCCTATATTCGACCGGGAGGAAGTCGCATACCAGGACACTGGGTTCAGCCTCCTCCTCCAGCCAACGAGATATGGGCATCTTAA
Protein sequence

>Msa0133310

ETDAAKDAIATAEESSKAMQNRGKLSKESNDESQIKENNRQIVHSVAEEGLDATDEKKSSNIASEGQNYGGAYDPLANLPIEFYHYYHGSNNDMGTLIEVRIGWDAYIRPGGSRIPGHWVQPPPPANEIWAS*