AlfalfaGEDB | Msa0135580

M. sativa cultivar zhongmu-4 / Msa0135580

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564
PFAM: PF01657.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0135580	sp\|Q9M0X5\|CRK25_ARATH	45.833	672	335	15	18	670	14	675	0.0	572

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0135580	A0A396JUV2	94.094	508	29	1	1	508	13	519	0.0	962

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0040990	Msa0135580	0.887283	1.634725e-72	-8.615850e-47
Msa0135580	Msa1008380	0.801175	1.019454e-48	-8.615850e-47
Msa0135580	Msa1008390	0.829769	4.276661e-55	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0135580	MtrunA17_Chr1g0206291	94.094	508	29	1	1	508	13	519	0.0	962
Msa0135580	MtrunA17_Chr1g0206351	52.665	638	280	8	26	647	27	658	0.0	684
Msa0135580	MtrunA17_Chr6g0475111	54.518	653	268	11	28	670	281	914	0.0	681
Msa0135580	MtrunA17_Chr6g0475111	50.607	247	116	5	9	253	10	252	1.81e-65	232
Msa0135580	MtrunA17_Chr8g0347571	53.425	657	267	14	28	670	266	897	0.0	666
Msa0135580	MtrunA17_Chr8g0347571	48.583	247	118	4	9	255	7	244	9.62e-63	224
Msa0135580	MtrunA17_Chr6g0474901	52.632	646	284	10	28	670	268	894	0.0	656
Msa0135580	MtrunA17_Chr6g0474901	48.162	272	122	8	8	274	6	263	9.36e-68	238
Msa0135580	MtrunA17_Chr6g0474861	53.083	665	254	9	8	670	6	614	0.0	647
Msa0135580	MtrunA17_Chr6g0474811	52.167	646	285	9	28	670	277	901	0.0	645

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0135580	AT4G05200.2	46.131	672	333	16	18	670	32	693	0.0	572
Msa0135580	AT4G05200.1	45.833	672	335	15	18	670	14	675	0.0	572
Msa0135580	AT4G23180.1	46.728	657	315	14	28	670	34	669	0.0	546
Msa0135580	AT4G23160.1	43.465	658	346	12	26	670	38	682	0.0	533
Msa0135580	AT4G23270.2	43.137	663	352	10	12	670	4	645	5.62e-172	506
Msa0135580	AT4G23270.1	43.137	663	352	10	12	670	4	645	5.62e-172	506
Msa0135580	AT4G23310.2	43.304	672	335	15	28	670	136	790	2.27e-171	509
Msa0135580	AT4G23310.2	29.911	224	146	7	28	247	23	239	4.52e-16	82.8
Msa0135580	AT4G23150.1	43.293	656	344	14	26	670	21	659	1.82e-170	503
Msa0135580	AT4G23270.3	43.030	660	351	10	12	667	4	642	2.06e-170	502

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Find 106 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTGGTAGAGAAAATTT+GGG	0.443943	1_3:-67246232	None:intergenic
AAAGTAAGAGAACGATAGAA+AGG	0.477714	1_3:-67246893	None:intergenic
AAATAATCAAGACTTCCATT+TGG	0.418479	1_3:-67248767	None:intergenic
AACACAAATGCTGGTTCTTG+TGG	0.572328	1_3:-67250282	None:intergenic
AACAGTGCACTCTTTATTTG+TGG	0.388488	1_3:-67246480	None:intergenic
AACCCTAAAATCTCAGATTT+TGG	0.174005	1_3:+67249646	Msa0135580:CDS
AACCTATGCACATCATGCGT+CGG	0.577967	1_3:+67246437	Msa0135580:CDS
AAGATTGGAAAAGGTGGATT+TGG	0.290249	1_3:+67247997	Msa0135580:CDS
AAGATTTCTATGTTGTAGCT+TGG	0.491023	1_3:-67248684	None:intergenic
AAGTAAGAGAACGATAGAAA+GGG	0.568732	1_3:-67246892	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGATGATTGAAATGGTTAAG+AGG	-	chr1_3:67246178-67246197	None:intergenic	30.0%
ATTGAGGAAGATGATTGAAA+TGG	-	chr1_3:67246186-67246205	None:intergenic	30.0%
GAGAATATGTTGCAAAATTG+AGG	-	chr1_3:67246202-67246221	None:intergenic	30.0%
CAAACTTGGTAGAGAAAATT+TGG	-	chr1_3:67246236-67246255	None:intergenic	30.0%
TCAACGTATCATACAACAAA+CGG	-	chr1_3:67246640-67246659	None:intergenic	30.0%
TTCGAAGAAATTCTCTACAA+GGG	+	chr1_3:67246688-67246707	Msa0135580:CDS	30.0%
TGATAAATTCAGATCTTCCA+AGG	+	chr1_3:67246804-67246823	Msa0135580:CDS	30.0%
AAGTAAGAGAACGATAGAAA+GGG	-	chr1_3:67246895-67246914	None:intergenic	30.0%
AAAGTAAGAGAACGATAGAA+AGG	-	chr1_3:67246896-67246915	None:intergenic	30.0%
TTTCTATCGTTCTCTTACTT+TGG	+	chr1_3:67246895-67246914	Msa0135580:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	67246143	67250425	67246143	ID=Msa0135580;Name=Msa0135580
chr1_3	mRNA	67246143	67250425	67246143	ID=Msa0135580-mRNA-1;Parent=Msa0135580;Name=Msa0135580-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0\|0.83\|0.57\|1\|1\|1\|7\|0\|670
chr1_3	exon	67246143	67246953	67246143	ID=Msa0135580-mRNA-1:exon:23088;Parent=Msa0135580-mRNA-1
chr1_3	exon	67247431	67247574	67247431	ID=Msa0135580-mRNA-1:exon:23089;Parent=Msa0135580-mRNA-1
chr1_3	exon	67247911	67248032	67247911	ID=Msa0135580-mRNA-1:exon:23090;Parent=Msa0135580-mRNA-1
chr1_3	exon	67248587	67248797	67248587	ID=Msa0135580-mRNA-1:exon:23091;Parent=Msa0135580-mRNA-1
chr1_3	exon	67249497	67249731	67249497	ID=Msa0135580-mRNA-1:exon:23092;Parent=Msa0135580-mRNA-1
chr1_3	exon	67249809	67249962	67249809	ID=Msa0135580-mRNA-1:exon:23093;Parent=Msa0135580-mRNA-1
chr1_3	exon	67250090	67250425	67250090	ID=Msa0135580-mRNA-1:exon:23094;Parent=Msa0135580-mRNA-1
chr1_3	CDS	67246143	67246953	67246143	ID=Msa0135580-mRNA-1:cds;Parent=Msa0135580-mRNA-1
chr1_3	CDS	67247431	67247574	67247431	ID=Msa0135580-mRNA-1:cds;Parent=Msa0135580-mRNA-1
chr1_3	CDS	67247911	67248032	67247911	ID=Msa0135580-mRNA-1:cds;Parent=Msa0135580-mRNA-1
chr1_3	CDS	67248587	67248797	67248587	ID=Msa0135580-mRNA-1:cds;Parent=Msa0135580-mRNA-1
chr1_3	CDS	67249497	67249731	67249497	ID=Msa0135580-mRNA-1:cds;Parent=Msa0135580-mRNA-1
chr1_3	CDS	67249809	67249962	67249809	ID=Msa0135580-mRNA-1:cds;Parent=Msa0135580-mRNA-1
chr1_3	CDS	67250090	67250425	67250090	ID=Msa0135580-mRNA-1:cds;Parent=Msa0135580-mRNA-1

Gene Sequence

>Msa0135580

ATGGCTTCCAACATCGAAGTATTAGTCTTCTTCCTCTTAACCATTTCAATCATCTTCCTCAATTTTGCAACATATTCTCATGCACAAGACCCAAATTTTCTCTACCAAGTTTGCTCAAAAAACAATTTCACATCCAACAGCACCTACCAAACAAACCTCAAAACTCTCTTCTCTTCCTTAACTTCCAAAGCAAAAACCGATCAAAACACCCAATTCTTCAACAACACCGTGGCCGGGAAAAATAATTCAGAAACAGTTTATGGACTATATATGTGCAGAGGTGACATCCCTTCCAACCTATGCACATCATGCGTCGGAAACGCAACTCAAAGATTGTCCACAAATAAAGAGTGCACTGTTTCAGTTGCAGCAGTTATGTGGTACGATGAATGCATGGTTCGGTATTCCAACATATCATTCTTTTCAACAGTTGCAACAGGACCAGGCTATGTTCTCCCAAGTCCAACTAACATGACAAACCAAGAAAGCTTCAACCGTTTGTTGTATGATACGTTGAACAAAACTTCAGATGAAGCTTCTTCTTCTTCGAAGAAATTCTCTACAAGGGAAGCGAAAATAAATATATTTCAGAATCTTTATTGTTTAGCTCAGTGTACACAAGATATTGAAGAAAGAGAGTGTAGAAGTTGTCTTTATGGTTTGATAAATTCAGATCTTCCAAGGTGTTGTGCAGGAACGCAAGGTGGAAGAGTTTTGTATCCTAATTGTATTATAAGGTTTGAAATTTACCCTTTCTATCGTTCTCTTACTTTGGCACCAACTCCAGCGCCTGCAGGACCTAATTCGACAGAAAATAAAAAAGGAAAATCACGAATAGCTATCATTGTTGCTATACCCATTGTGGCTGCTTCTGCAATAGTCTTTGCTGTTTGCTTGTATTATCGAAGGAGAAAAGCGAGAACAAGCAGGTCCAATATTCTCCTCCAAGAAAACTTTGGACGTGAAAGTACCACTTTAGAGGGGTTGCAATTCGATTTAGCTACAATTGCTACAGCAACAAACAATTTCTCACATGAGAACAAGATTGGAAAAGGTGGATTTGGAGAAGTTTACAAGGGCACTCTTCGTGATGGACGAGATATAGCTGTCAAGAGGCTTTCAACAAGTTCCACACAAGGTTCGATTGAGTTCAAGAATGAGATTTTATTGATAGCCAAGCTACAACATAGAAATCTTGTGGCATTGATAGGTTTTTGCCTAGAAGAGCAAGAGAAGATTCTCATTTACGAATATGTCCCAAATGGAAGTCTTGATTATTTTCTATTCGGGGCTCAAGAACAAAAGTTAAGCTGGTCCGAGCGCTACAAAATTATAGGAGGAACTGCTTTAGGAGTTCTTTATTTACACGATTATTCTCGACTAAAAGTTATACATCGTGATCTTAAACCTAGTAATATTCTATTAGATGAACATATGAACCCTAAAATCTCAGATTTTGGCATGGCTAGAATTGTTGACATTGACCAAGATCGCGGTAAAACCACCAGAATTGTGGGAACATATGGTTATATGTCTCCAGAATATGCAATGCTTGGAAAGTTTTCCGAAAAATCAGATGTTTTTAGTTTTGGAGTAATGGTCATAGAGATAATCACTGGGAAAAGGAATGCTAAGTCTTACGAATCAAATCCGGATGCAAAAGGCCTCTTGAGCTATGTTTGGAGACAATGGAATGACCAAACATTGTTCACTGCAATTGACCCAGATATAAAAGAAAAATATTCTGAAATTGAAGTTATAAAATGCATTCAAATTGGTTTATTATGTGTCCAAGAAAATCCAAATGCTAGACCTACAATGGCAACAGTCGTTTCATATCTTACTAATCACTCACTTGAACTGCCATTTCCACAAGAACCAGCATTTGTGTTGCATAGTACAATTGATCAAAAAATTGTTGGGCAGCAGGAGTCAAGCTCAGGTCTATCTGCTAGTAGTTCTAAACCATTTTCTGTTAATGACATGTCAATAAGTACATTTTATCCTCGTTAA

Protein sequence

>Msa0135580

MASNIEVLVFFLLTISIIFLNFATYSHAQDPNFLYQVCSKNNFTSNSTYQTNLKTLFSSLTSKAKTDQNTQFFNNTVAGKNNSETVYGLYMCRGDIPSNLCTSCVGNATQRLSTNKECTVSVAAVMWYDECMVRYSNISFFSTVATGPGYVLPSPTNMTNQESFNRLLYDTLNKTSDEASSSSKKFSTREAKINIFQNLYCLAQCTQDIEERECRSCLYGLINSDLPRCCAGTQGGRVLYPNCIIRFEIYPFYRSLTLAPTPAPAGPNSTENKKGKSRIAIIVAIPIVAASAIVFAVCLYYRRRKARTSRSNILLQENFGRESTTLEGLQFDLATIATATNNFSHENKIGKGGFGEVYKGTLRDGRDIAVKRLSTSSTQGSIEFKNEILLIAKLQHRNLVALIGFCLEEQEKILIYEYVPNGSLDYFLFGAQEQKLSWSERYKIIGGTALGVLYLHDYSRLKVIHRDLKPSNILLDEHMNPKISDFGMARIVDIDQDRGKTTRIVGTYGYMSPEYAMLGKFSEKSDVFSFGVMVIEIITGKRNAKSYESNPDAKGLLSYVWRQWNDQTLFTAIDPDIKEKYSEIEVIKCIQIGLLCVQENPNARPTMATVVSYLTNHSLELPFPQEPAFVLHSTIDQKIVGQQESSSGLSASSSKPFSVNDMSISTFYPR*