Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0135760 | Msa0135770 | 0.838720 | 2.431978e-57 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 5 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTATATGTTTATCAGTGTA+AGG | 0.390449 | 1_3:+67501966 | Msa0135770:CDS |
| CTTACACTGATAAACATATA+CGG | 0.395159 | 1_3:-67501965 | None:intergenic |
| ATAAGCACATCTGCTTCATT+AGG | 0.401944 | 1_3:+67502019 | Msa0135770:CDS |
| CAGTACTTCAAGAGAAGTGT+TGG | 0.501193 | 1_3:-67500706 | None:intergenic |
| AGTACTTCAAGAGAAGTGTT+GGG | 0.559445 | 1_3:-67500705 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAATAAAATTAAAATCT+TGG | - | chr1_3:67500955-67500974 | None:intergenic | 10.0% |
| !!! | ATATATTTTGACATAAAAAA+TGG | + | chr1_3:67501482-67501501 | Msa0135770:intron | 10.0% |
| !!! | TATTTATTAATTTGTGATTT+AGG | + | chr1_3:67501548-67501567 | Msa0135770:intron | 10.0% |
| !! | ACAAAATAAAATTAAAATCT+TGG | - | chr1_3:67500955-67500974 | None:intergenic | 10.0% |
| !!! | ATATATTTTGACATAAAAAA+TGG | + | chr1_3:67501482-67501501 | Msa0135770:intron | 10.0% |
| !!! | TATTTATTAATTTGTGATTT+AGG | + | chr1_3:67501548-67501567 | Msa0135770:intron | 10.0% |
| !! | TTTATGAAAATATATGTGAA+TGG | + | chr1_3:67501299-67501318 | Msa0135770:intron | 15.0% |
| !! | AATATCTAAGTTAATTTACT+AGG | + | chr1_3:67501752-67501771 | Msa0135770:intron | 15.0% |
| !! | GATATTTAATTAAATGACTA+AGG | - | chr1_3:67501814-67501833 | None:intergenic | 15.0% |
| !!! | TTTTTTTCTTTTTCAGATAA+AGG | + | chr1_3:67501853-67501872 | Msa0135770:intron | 15.0% |
| !!! | AACATGAGTAATAATTTTTT+GGG | - | chr1_3:67501927-67501946 | None:intergenic | 15.0% |
| !! | TTTATGAAAATATATGTGAA+TGG | + | chr1_3:67501299-67501318 | Msa0135770:intron | 15.0% |
| !! | AATATCTAAGTTAATTTACT+AGG | + | chr1_3:67501752-67501771 | Msa0135770:intron | 15.0% |
| !! | GATATTTAATTAAATGACTA+AGG | - | chr1_3:67501814-67501833 | None:intergenic | 15.0% |
| !!! | TTTTTTTCTTTTTCAGATAA+AGG | + | chr1_3:67501853-67501872 | Msa0135770:intron | 15.0% |
| !!! | AACATGAGTAATAATTTTTT+GGG | - | chr1_3:67501927-67501946 | None:intergenic | 15.0% |
| !! | TCAGTAATATCTTTATAAGA+GGG | - | chr1_3:67500886-67500905 | None:intergenic | 20.0% |
| !! | TATATATTAAAGGAGAGAAA+TGG | + | chr1_3:67501148-67501167 | Msa0135770:intron | 20.0% |
| !!! | TATTTTGACATAAAAAATGG+AGG | + | chr1_3:67501485-67501504 | Msa0135770:intron | 20.0% |
| !!! | ATTTTGACATAAAAAATGGA+GGG | + | chr1_3:67501486-67501505 | Msa0135770:intron | 20.0% |
| !! | ATATCATAGCCTATTTATTA+AGG | - | chr1_3:67501639-67501658 | None:intergenic | 20.0% |
| !!! | TGTCATGTCTTATTTATTTT+CGG | + | chr1_3:67501685-67501704 | Msa0135770:intron | 20.0% |
| !!! | TTATTTATTTTCGGTTCAAT+TGG | + | chr1_3:67501694-67501713 | Msa0135770:intron | 20.0% |
| !!! | TATTTATTTTCGGTTCAATT+GGG | + | chr1_3:67501695-67501714 | Msa0135770:intron | 20.0% |
| !! | GAAAAAAAATCATCAAATCT+CGG | - | chr1_3:67501841-67501860 | None:intergenic | 20.0% |
| !!! | CAACATGAGTAATAATTTTT+TGG | - | chr1_3:67501928-67501947 | None:intergenic | 20.0% |
| !! | TCAGTAATATCTTTATAAGA+GGG | - | chr1_3:67500886-67500905 | None:intergenic | 20.0% |
| !! | TATATATTAAAGGAGAGAAA+TGG | + | chr1_3:67501148-67501167 | Msa0135770:intron | 20.0% |
| !!! | TATTTTGACATAAAAAATGG+AGG | + | chr1_3:67501485-67501504 | Msa0135770:intron | 20.0% |
| !!! | ATTTTGACATAAAAAATGGA+GGG | + | chr1_3:67501486-67501505 | Msa0135770:intron | 20.0% |
| !! | ATATCATAGCCTATTTATTA+AGG | - | chr1_3:67501639-67501658 | None:intergenic | 20.0% |
| !!! | TGTCATGTCTTATTTATTTT+CGG | + | chr1_3:67501685-67501704 | Msa0135770:intron | 20.0% |
| !!! | TTATTTATTTTCGGTTCAAT+TGG | + | chr1_3:67501694-67501713 | Msa0135770:intron | 20.0% |
| !!! | TATTTATTTTCGGTTCAATT+GGG | + | chr1_3:67501695-67501714 | Msa0135770:intron | 20.0% |
| !! | GAAAAAAAATCATCAAATCT+CGG | - | chr1_3:67501841-67501860 | None:intergenic | 20.0% |
| !!! | CAACATGAGTAATAATTTTT+TGG | - | chr1_3:67501928-67501947 | None:intergenic | 20.0% |
| ! | CAGTAATATCTTTATAAGAG+GGG | - | chr1_3:67500885-67500904 | None:intergenic | 25.0% |
| ! | CTCAGTAATATCTTTATAAG+AGG | - | chr1_3:67500887-67500906 | None:intergenic | 25.0% |
| ! | CATAAAAAGTTACCATTCAA+AGG | - | chr1_3:67501285-67501304 | None:intergenic | 25.0% |
| ! | AATATATGTAATCAGTGCAT+TGG | + | chr1_3:67501336-67501355 | Msa0135770:intron | 25.0% |
| ! | TAAAGATCTCTTAAGAAAGA+CGG | + | chr1_3:67501514-67501533 | Msa0135770:intron | 25.0% |
| ! | CTTACACTGATAAACATATA+CGG | - | chr1_3:67501968-67501987 | None:intergenic | 25.0% |
| ! | CAGTAATATCTTTATAAGAG+GGG | - | chr1_3:67500885-67500904 | None:intergenic | 25.0% |
| ! | CTCAGTAATATCTTTATAAG+AGG | - | chr1_3:67500887-67500906 | None:intergenic | 25.0% |
| ! | CATAAAAAGTTACCATTCAA+AGG | - | chr1_3:67501285-67501304 | None:intergenic | 25.0% |
| ! | AATATATGTAATCAGTGCAT+TGG | + | chr1_3:67501336-67501355 | Msa0135770:intron | 25.0% |
| ! | TAAAGATCTCTTAAGAAAGA+CGG | + | chr1_3:67501514-67501533 | Msa0135770:intron | 25.0% |
| ! | CTTACACTGATAAACATATA+CGG | - | chr1_3:67501968-67501987 | None:intergenic | 25.0% |
| GTTGATGTGTCATATTTCAT+CGG | + | chr1_3:67500915-67500934 | Msa0135770:intron | 30.0% | |
| ! | GTTATTCTAGGGAAATGTTA+AGG | + | chr1_3:67501234-67501253 | Msa0135770:intron | 30.0% |
| !! | TGTCTCTGAAATACTCTTTA+AGG | - | chr1_3:67501410-67501429 | None:intergenic | 30.0% |
| GCATGAATATGTGGATAATT+GGG | + | chr1_3:67501609-67501628 | Msa0135770:intron | 30.0% | |
| CATGAATATGTGGATAATTG+GGG | + | chr1_3:67501610-67501629 | Msa0135770:intron | 30.0% | |
| CGTATATGTTTATCAGTGTA+AGG | + | chr1_3:67501966-67501985 | Msa0135770:CDS | 30.0% | |
| GTTGATGTGTCATATTTCAT+CGG | + | chr1_3:67500915-67500934 | Msa0135770:intron | 30.0% | |
| ! | GTTATTCTAGGGAAATGTTA+AGG | + | chr1_3:67501234-67501253 | Msa0135770:intron | 30.0% |
| !! | TGTCTCTGAAATACTCTTTA+AGG | - | chr1_3:67501410-67501429 | None:intergenic | 30.0% |
| GCATGAATATGTGGATAATT+GGG | + | chr1_3:67501609-67501628 | Msa0135770:intron | 30.0% | |
| CATGAATATGTGGATAATTG+GGG | + | chr1_3:67501610-67501629 | Msa0135770:intron | 30.0% | |
| CGTATATGTTTATCAGTGTA+AGG | + | chr1_3:67501966-67501985 | Msa0135770:CDS | 30.0% | |
| !! | AGTACTTCAAGAGAAGTGTT+GGG | - | chr1_3:67500708-67500727 | None:intergenic | 35.0% |
| !! | CTTCTCTTGAAGTACTGTTA+TGG | + | chr1_3:67500712-67500731 | Msa0135770:intron | 35.0% |
| ! | GAGGATAGTGTGTTATTCTA+GGG | + | chr1_3:67501223-67501242 | Msa0135770:intron | 35.0% |
| !! | CACTCTTTAAGACCTTTGAA+TGG | + | chr1_3:67501270-67501289 | Msa0135770:intron | 35.0% |
| CTCTTCCGTGTATTATTGTT+GGG | + | chr1_3:67501587-67501606 | Msa0135770:intron | 35.0% | |
| TCATGCCCAACAATAATACA+CGG | - | chr1_3:67501595-67501614 | None:intergenic | 35.0% | |
| TATTGTTGGGCATGAATATG+TGG | + | chr1_3:67501600-67501619 | Msa0135770:intron | 35.0% | |
| GGCATGAATATGTGGATAAT+TGG | + | chr1_3:67501608-67501627 | Msa0135770:intron | 35.0% | |
| TTGGGGTGACCTTAATAAAT+AGG | + | chr1_3:67501627-67501646 | Msa0135770:intron | 35.0% | |
| ATAAGCACATCTGCTTCATT+AGG | + | chr1_3:67502019-67502038 | Msa0135770:CDS | 35.0% | |
| !! | AGTACTTCAAGAGAAGTGTT+GGG | - | chr1_3:67500708-67500727 | None:intergenic | 35.0% |
| !! | CTTCTCTTGAAGTACTGTTA+TGG | + | chr1_3:67500712-67500731 | Msa0135770:intron | 35.0% |
| ! | GAGGATAGTGTGTTATTCTA+GGG | + | chr1_3:67501223-67501242 | Msa0135770:intron | 35.0% |
| !! | CACTCTTTAAGACCTTTGAA+TGG | + | chr1_3:67501270-67501289 | Msa0135770:intron | 35.0% |
| CTCTTCCGTGTATTATTGTT+GGG | + | chr1_3:67501587-67501606 | Msa0135770:intron | 35.0% | |
| TCATGCCCAACAATAATACA+CGG | - | chr1_3:67501595-67501614 | None:intergenic | 35.0% | |
| TATTGTTGGGCATGAATATG+TGG | + | chr1_3:67501600-67501619 | Msa0135770:intron | 35.0% | |
| GGCATGAATATGTGGATAAT+TGG | + | chr1_3:67501608-67501627 | Msa0135770:intron | 35.0% | |
| TTGGGGTGACCTTAATAAAT+AGG | + | chr1_3:67501627-67501646 | Msa0135770:intron | 35.0% | |
| ATAAGCACATCTGCTTCATT+AGG | + | chr1_3:67502019-67502038 | Msa0135770:CDS | 35.0% | |
| ! | CAGTACTTCAAGAGAAGTGT+TGG | - | chr1_3:67500709-67500728 | None:intergenic | 40.0% |
| ! | GGAGGATAGTGTGTTATTCT+AGG | + | chr1_3:67501222-67501241 | Msa0135770:intron | 40.0% |
| !! | AAATGTTAAGGAGTGTCTCC+AGG | + | chr1_3:67501246-67501265 | Msa0135770:intron | 40.0% |
| !! | AATGTTAAGGAGTGTCTCCA+GGG | + | chr1_3:67501247-67501266 | Msa0135770:intron | 40.0% |
| CCAACAATAATACACGGAAG+AGG | - | chr1_3:67501589-67501608 | None:intergenic | 40.0% | |
| CCTCTTCCGTGTATTATTGT+TGG | + | chr1_3:67501586-67501605 | Msa0135770:intron | 40.0% | |
| ! | CAGTACTTCAAGAGAAGTGT+TGG | - | chr1_3:67500709-67500728 | None:intergenic | 40.0% |
| ! | GGAGGATAGTGTGTTATTCT+AGG | + | chr1_3:67501222-67501241 | Msa0135770:intron | 40.0% |
| !! | AAATGTTAAGGAGTGTCTCC+AGG | + | chr1_3:67501246-67501265 | Msa0135770:intron | 40.0% |
| !! | AATGTTAAGGAGTGTCTCCA+GGG | + | chr1_3:67501247-67501266 | Msa0135770:intron | 40.0% |
| CCAACAATAATACACGGAAG+AGG | - | chr1_3:67501589-67501608 | None:intergenic | 40.0% | |
| CCTCTTCCGTGTATTATTGT+TGG | + | chr1_3:67501586-67501605 | Msa0135770:intron | 40.0% | |
| !! | AAAGGTCTTAAAGAGTGCCC+TGG | - | chr1_3:67501267-67501286 | None:intergenic | 45.0% |
| !! | AAAGGTCTTAAAGAGTGCCC+TGG | - | chr1_3:67501267-67501286 | None:intergenic | 45.0% |
| !! | AATAAAGAAATATATATTAA+AGG | + | chr1_3:67501138-67501157 | Msa0135770:intron | 5.0% |
| !! | AATTATAAAATTATAAAACA+TGG | - | chr1_3:67501789-67501808 | None:intergenic | 5.0% |
| !! | AATAAAGAAATATATATTAA+AGG | + | chr1_3:67501138-67501157 | Msa0135770:intron | 5.0% |
| !! | AATTATAAAATTATAAAACA+TGG | - | chr1_3:67501789-67501808 | None:intergenic | 5.0% |
| GTAATGCAAGCTCCTCTCTC+AGG | + | chr1_3:67501201-67501220 | Msa0135770:intron | 50.0% | |
| ACACACTATCCTCCTGAGAG+AGG | - | chr1_3:67501216-67501235 | None:intergenic | 50.0% | |
| GTAATGCAAGCTCCTCTCTC+AGG | + | chr1_3:67501201-67501220 | Msa0135770:intron | 50.0% | |
| ACACACTATCCTCCTGAGAG+AGG | - | chr1_3:67501216-67501235 | None:intergenic | 50.0% | |
| ATGCAAGCTCCTCTCTCAGG+AGG | + | chr1_3:67501204-67501223 | Msa0135770:intron | 55.0% | |
| ATGCAAGCTCCTCTCTCAGG+AGG | + | chr1_3:67501204-67501223 | Msa0135770:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_3 | gene | 67500699 | 67502069 | 67500699 | ID=Msa0135770;Name=Msa0135770 |
| chr1_3 | mRNA | 67500699 | 67502069 | 67500699 | ID=Msa0135770-mRNA-1;Parent=Msa0135770;Name=Msa0135770-mRNA-1;_AED=0.44;_eAED=0.50;_QI=0|0|0|1|0|0|2|0|74 |
| chr1_3 | exon | 67500699 | 67500722 | 67500699 | ID=Msa0135770-mRNA-1:exon:23177;Parent=Msa0135770-mRNA-1 |
| chr1_3 | exon | 67501869 | 67502069 | 67501869 | ID=Msa0135770-mRNA-1:exon:23178;Parent=Msa0135770-mRNA-1 |
| chr1_3 | CDS | 67500699 | 67500722 | 67500699 | ID=Msa0135770-mRNA-1:cds;Parent=Msa0135770-mRNA-1 |
| chr1_3 | CDS | 67501869 | 67502069 | 67501869 | ID=Msa0135770-mRNA-1:cds;Parent=Msa0135770-mRNA-1 |
| Gene Sequence |
| Protein sequence |
