AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0136140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0136140 MtrunA17_Chr1g0207301 95.758 330 14 0 1 330 51 380 0.0 649
Msa0136140 MtrunA17_Chr5g0393721 49.699 332 131 10 9 329 9 315 2.79e-86 263
Msa0136140 MtrunA17_Chr1g0207311 43.202 331 132 9 1 324 1 282 4.64e-61 196
Msa0136140 MtrunA17_Chr3g0134741 52.147 163 77 1 161 323 153 314 5.24e-48 164
Msa0136140 MtrunA17_Chr1g0152651 51.235 162 69 2 152 313 198 349 1.20e-40 145
Msa0136140 MtrunA17_Chr5g0435021 62.857 70 26 0 237 306 217 286 6.20e-24 99.8
Msa0136140 MtrunA17_Chr8g0384141 53.623 69 32 0 234 302 110 178 3.00e-18 83.2
Msa0136140 MtrunA17_Chr4g0074161 43.333 90 49 1 214 301 180 269 6.23e-17 80.9
Msa0136140 MtrunA17_Chr3g0134001 49.254 67 34 0 234 300 167 233 8.65e-17 79.0
Msa0136140 MtrunA17_Chr7g0243041 58.182 55 23 0 245 299 33 87 2.64e-16 75.1
Msa0136140 MtrunA17_Chr1g0190731 47.059 68 36 0 234 301 189 256 3.84e-14 72.4
Msa0136140 MtrunA17_Chr1g0153241 43.548 62 35 0 254 315 101 162 1.29e-12 67.0
Msa0136140 MtrunA17_Chr5g0402031 42.105 76 44 0 254 329 121 196 2.68e-12 67.0
Msa0136140 MtrunA17_Chr1g0193321 50.000 48 24 0 254 301 478 525 8.74e-12 66.2
Msa0136140 MtrunA17_Chr3g0095391 36.264 91 46 3 224 307 288 373 2.01e-11 64.7
Msa0136140 MtrunA17_Chr1g0182791 37.349 83 45 1 226 301 298 380 5.45e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0136140 AT2G14760.1 37.216 352 150 12 10 324 6 323 6.31e-56 185
Msa0136140 AT2G14760.3 35.890 365 150 13 10 324 6 336 3.33e-52 176
Msa0136140 AT4G33880.1 38.136 354 165 16 10 320 6 348 2.55e-45 158
Msa0136140 AT5G43175.1 54.140 157 57 2 184 325 63 219 5.98e-45 153
Msa0136140 AT1G27740.1 53.642 151 52 3 176 325 121 254 1.22e-44 153
Msa0136140 AT1G66470.1 54.839 93 41 1 214 306 172 263 1.66e-24 101
Msa0136140 AT5G37800.1 61.039 77 27 1 230 306 196 269 1.73e-24 101
Msa0136140 AT4G30980.1 45.783 83 45 0 219 301 111 193 1.72e-17 82.0
Msa0136140 AT4G30980.2 45.783 83 45 0 219 301 159 241 1.92e-17 82.4
Msa0136140 AT2G24260.2 53.846 65 30 0 237 301 34 98 4.32e-17 79.7
Msa0136140 AT2G24260.1 53.846 65 30 0 237 301 137 201 9.97e-17 80.5
Msa0136140 AT5G01305.1 48.649 74 33 1 237 305 36 109 2.40e-16 76.3
Msa0136140 AT3G21330.1 39.394 99 59 1 202 300 234 331 3.57e-16 79.0
Msa0136140 AT5G58010.1 47.143 70 37 0 232 301 93 162 7.95e-16 77.0
Msa0136140 AT4G00120.1 37.975 79 49 0 222 300 96 174 3.36e-15 73.6
Msa0136140 AT4G36930.1 39.189 74 45 0 245 318 198 271 5.56e-12 66.6
Msa0136140 AT2G20180.7 40.244 82 38 2 254 326 223 302 5.58e-12 66.6
Msa0136140 AT2G20180.1 40.244 82 38 2 254 326 223 302 5.58e-12 66.6
Msa0136140 AT2G20180.5 40.244 82 38 2 254 326 223 302 5.58e-12 66.6
Msa0136140 AT1G09530.3 39.394 66 40 0 254 319 353 418 9.28e-12 66.2
Msa0136140 AT1G09530.2 39.394 66 40 0 254 319 353 418 9.28e-12 66.2
Msa0136140 AT1G09530.5 39.394 66 40 0 254 319 353 418 9.28e-12 66.2
Msa0136140 AT1G09530.1 39.394 66 40 0 254 319 353 418 9.28e-12 66.2
Msa0136140 AT1G09530.4 39.394 66 40 0 254 319 353 418 9.28e-12 66.2
Msa0136140 AT1G09530.6 39.394 66 40 0 254 319 353 418 9.28e-12 66.2
Msa0136140 AT2G20180.4 40.244 82 38 2 254 326 294 373 9.87e-12 66.2
Msa0136140 AT2G20180.6 40.244 82 38 2 254 326 294 373 9.87e-12 66.2
Msa0136140 AT2G20180.2 40.244 82 38 2 254 326 294 373 9.87e-12 66.2
Msa0136140 AT2G20180.3 40.244 82 38 2 254 326 294 373 9.87e-12 66.2
Msa0136140 AT5G67110.1 42.857 77 28 3 254 330 103 163 9.22e-11 61.2

Find 54 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAACAGATTTGAAGTTAA+AGG 0.203807 1_3:-67936820 Msa0136140:CDS
TCAAATCTGTTGAATCATTC+TGG 0.235168 1_3:+67936829 None:intergenic
CATTAGGGACTAGGCTTTGC+AGG 0.277798 1_3:+67936572 None:intergenic
CTTGGAAGTTATTAAGTAAC+TGG 0.304266 1_3:+67937696 None:intergenic
AACACTATAAGTGCTAAAAT+TGG 0.312512 1_3:+67937427 None:intergenic
TTCATTCCATTGTAAGCAAT+TGG 0.326950 1_3:+67936211 None:intergenic
CAAGTTTCTGAGCACCATTA+TGG 0.327811 1_3:-67937677 Msa0136140:CDS
TCAACAGATTTGAAGTTAAA+GGG 0.332099 1_3:-67936819 Msa0136140:CDS
AGAGTTTGGTAAAGATGTTT+TGG 0.332649 1_3:-67937363 Msa0136140:CDS
CTAAATTGAAATTTCCATAA+TGG 0.336286 1_3:+67937663 None:intergenic
CCTTGGTTCCATTAGGGACT+AGG 0.345834 1_3:+67936563 None:intergenic
CATAATGGTGCTCAGAAACT+TGG 0.346093 1_3:+67937678 None:intergenic
GAAAACACTTCTGAAGAGTT+TGG 0.361815 1_3:-67937377 Msa0136140:CDS
CAAGGAAAGAAAATGGATTC+TGG 0.368940 1_3:-67937788 None:intergenic
TTTAGCACTTATAGTGTTCA+AGG 0.374420 1_3:-67937422 Msa0136140:CDS
AAAGCTACCTTGGTTCCATT+AGG 0.376583 1_3:+67936556 None:intergenic
GGATGAAAGTCATGATCTTC+AGG 0.377305 1_3:-67936928 Msa0136140:CDS
ACAAGAGAAATGTGAAATCT+AGG 0.391427 1_3:-67936989 Msa0136140:CDS
TTTATTGCTAGTATCAGAAA+TGG 0.398251 1_3:+67937517 None:intergenic
AAGCTACCTTGGTTCCATTA+GGG 0.399329 1_3:+67936557 None:intergenic
GCTATATATCTATGGTAGTT+CGG 0.420297 1_3:+67936159 None:intergenic
CTGCAAAGCCTAGTCCCTAA+TGG 0.420311 1_3:-67936571 Msa0136140:CDS
AATAAATTTCATAATACAAA+TGG 0.447590 1_3:-67937500 Msa0136140:CDS
GAAGAATGGAGTTCTCTTAG+TGG 0.449677 1_3:-67937752 Msa0136140:CDS
ACTATTGTGGATTCATGTCC+TGG 0.460970 1_3:+67937620 None:intergenic
TATATCTATGGTAGTTCGGT+TGG 0.463306 1_3:+67936163 None:intergenic
TAGAAATTCCATCTACATCA+TGG 0.473913 1_3:-67937643 Msa0136140:CDS
TGTCCTGGCCATGATGTAGA+TGG 0.481256 1_3:+67937635 None:intergenic
GCTATTCATGCTCACTATTG+TGG 0.481463 1_3:+67937607 None:intergenic
AAGGGAAAATCAAAATGTAA+TGG 0.488727 1_3:-67936801 Msa0136140:CDS
GGCATTCATGTAGCCAATGT+TGG 0.502539 1_3:+67937448 None:intergenic
ATATCTATGGTAGTTCGGTT+GGG 0.502830 1_3:+67936164 None:intergenic
AAGATCTAGGAGCTCGATGA+AGG 0.519130 1_3:-67937294 Msa0136140:intron
TTCTGAGCTCTGATGATCAC+TGG 0.521996 1_3:-67936243 Msa0136140:CDS
GCTTACAATGGAATGAACAT+TGG 0.525606 1_3:-67936205 Msa0136140:CDS
GTGAGCAAAGTTGTGAAGAA+TGG 0.531367 1_3:-67937766 Msa0136140:CDS
GTTCGGTTGGGATAATGTTG+AGG 0.541601 1_3:+67936176 None:intergenic
AATGATCATGTAGCCAACAT+TGG 0.547548 1_3:-67937461 Msa0136140:CDS
ATTCCATCTACATCATGGCC+AGG 0.561187 1_3:-67937638 Msa0136140:CDS
GTTCAAAAGCAACGCTGACG+AGG 0.565933 1_3:-67936949 Msa0136140:CDS
GTTCTTGATACACTCTTTCG+CGG 0.570482 1_3:+67937337 None:intergenic
TCAGGAGCAGAATTTGAGCT+CGG 0.588142 1_3:-67936910 Msa0136140:CDS
GCTTTAAAGAAGCTAAATGC+AGG 0.589797 1_3:-67936870 Msa0136140:CDS
GGATATCAGCACCATGCTTG+AGG 0.592275 1_3:-67936453 Msa0136140:CDS
AAATTTCATAATACAAATGG+TGG 0.597992 1_3:-67937497 Msa0136140:CDS
TATGCTCCAATTGCTTACAA+TGG 0.606083 1_3:-67936217 Msa0136140:CDS
GGAAAATCAAAATGTAATGG+AGG 0.608687 1_3:-67936798 Msa0136140:CDS
TTGCACAGCTTCCTCAAGCA+TGG 0.610000 1_3:+67936442 None:intergenic
ATAGACACCTTGTGGATCAG+AGG 0.616647 1_3:+67936770 None:intergenic
ATCTAGGAAGAAACCAAAGT+CGG 0.617908 1_3:-67936973 Msa0136140:CDS
CCTAGTCCCTAATGGAACCA+AGG 0.646129 1_3:-67936563 Msa0136140:intron
GGTTCTGCCTCTGATCCACA+AGG 0.648330 1_3:-67936777 Msa0136140:CDS
GGAGCAGAATTTGAGCTCGG+AGG 0.653936 1_3:-67936907 Msa0136140:CDS
AGAGTGTATCAAGAACAACC+TGG 0.703200 1_3:-67937330 Msa0136140:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATTTCATAATACAAA+TGG - chr1_3:67936444-67936463 Msa0136140:CDS 10.0%
!!! TTCAACTATTTTTAAATAAT+TGG - chr1_3:67937422-67937441 Msa0136140:CDS 10.0%
!! TATTTCAAGAATATATAATA+TGG + chr1_3:67937634-67937653 None:intergenic 10.0%
!! AATAAATTTCATAATACAAA+TGG - chr1_3:67936444-67936463 Msa0136140:CDS 10.0%
!!! TTCAACTATTTTTAAATAAT+TGG - chr1_3:67937422-67937441 Msa0136140:CDS 10.0%
!! TATTTCAAGAATATATAATA+TGG + chr1_3:67937634-67937653 None:intergenic 10.0%
!! TAAATTTATGATTCATATCA+AGG + chr1_3:67937591-67937610 None:intergenic 15.0%
!! TAAATTTATGATTCATATCA+AGG + chr1_3:67937591-67937610 None:intergenic 15.0%
!! CTAAATTGAAATTTCCATAA+TGG + chr1_3:67936284-67936303 None:intergenic 20.0%
!! AAATTTCATAATACAAATGG+TGG - chr1_3:67936447-67936466 Msa0136140:CDS 20.0%
!! GGTATTATGTATAAGAAAAA+AGG - chr1_3:67936717-67936736 Msa0136140:intron 20.0%
!! AACAAATAGAAACATGTATT+TGG + chr1_3:67936767-67936786 None:intergenic 20.0%
!!! TATTTGTTTACATTTAAGAC+CGG - chr1_3:67936779-67936798 Msa0136140:CDS 20.0%
!!! TTTTGATTTAATGATTCCAT+AGG - chr1_3:67936923-67936942 Msa0136140:CDS 20.0%
!!! TTATTAGCAATGGTATTTTT+AGG + chr1_3:67937227-67937246 None:intergenic 20.0%
!!! CTGTGATTTTGAATTATTAA+AGG - chr1_3:67937277-67937296 Msa0136140:intron 20.0%
!!! TTTTCTTACTCATGAAATAT+AGG - chr1_3:67937467-67937486 Msa0136140:CDS 20.0%
!!! CTTATGTTTTTACCTTAATT+TGG + chr1_3:67937548-67937567 None:intergenic 20.0%
!! CAAATTAAGGTAAAAACATA+AGG - chr1_3:67937546-67937565 Msa0136140:CDS 20.0%
!! CTAAATTGAAATTTCCATAA+TGG + chr1_3:67936284-67936303 None:intergenic 20.0%
!! AAATTTCATAATACAAATGG+TGG - chr1_3:67936447-67936466 Msa0136140:CDS 20.0%
!! GGTATTATGTATAAGAAAAA+AGG - chr1_3:67936717-67936736 Msa0136140:intron 20.0%
!! AACAAATAGAAACATGTATT+TGG + chr1_3:67936767-67936786 None:intergenic 20.0%
!!! TATTTGTTTACATTTAAGAC+CGG - chr1_3:67936779-67936798 Msa0136140:CDS 20.0%
!!! TTTTGATTTAATGATTCCAT+AGG - chr1_3:67936923-67936942 Msa0136140:CDS 20.0%
!!! TTATTAGCAATGGTATTTTT+AGG + chr1_3:67937227-67937246 None:intergenic 20.0%
!!! CTGTGATTTTGAATTATTAA+AGG - chr1_3:67937277-67937296 Msa0136140:intron 20.0%
!!! TTTTCTTACTCATGAAATAT+AGG - chr1_3:67937467-67937486 Msa0136140:CDS 20.0%
!!! CTTATGTTTTTACCTTAATT+TGG + chr1_3:67937548-67937567 None:intergenic 20.0%
!! CAAATTAAGGTAAAAACATA+AGG - chr1_3:67937546-67937565 Msa0136140:CDS 20.0%
!! TTACTATTATCAGCATTTTG+AGG + chr1_3:67936375-67936394 None:intergenic 25.0%
! TTTATTGCTAGTATCAGAAA+TGG + chr1_3:67936430-67936449 None:intergenic 25.0%
! AACACTATAAGTGCTAAAAT+TGG + chr1_3:67936520-67936539 None:intergenic 25.0%
! AAAAATCAGGAAAAAGATCT+AGG - chr1_3:67936637-67936656 Msa0136140:intron 25.0%
! TTCTTATACATAATACCAGA+GGG + chr1_3:67936714-67936733 None:intergenic 25.0%
! TTTCTTATACATAATACCAG+AGG + chr1_3:67936715-67936734 None:intergenic 25.0%
!!! TCAATGTGTTTAATTTTGTC+TGG - chr1_3:67936880-67936899 Msa0136140:CDS 25.0%
!! TTCAACAGATTTGAAGTTAA+AGG - chr1_3:67937124-67937143 Msa0136140:intron 25.0%
!! TCAACAGATTTGAAGTTAAA+GGG - chr1_3:67937125-67937144 Msa0136140:intron 25.0%
! AAGGGAAAATCAAAATGTAA+TGG - chr1_3:67937143-67937162 Msa0136140:intron 25.0%
!!! GTATTTTTAGGAAATGCAAT+TGG + chr1_3:67937215-67937234 None:intergenic 25.0%
! GGACATAAAATTATTAGCAA+TGG + chr1_3:67937237-67937256 None:intergenic 25.0%
! AATTCAAAATCACAGTACTT+TGG + chr1_3:67937272-67937291 None:intergenic 25.0%
!! TTACTATTATCAGCATTTTG+AGG + chr1_3:67936375-67936394 None:intergenic 25.0%
! TTTATTGCTAGTATCAGAAA+TGG + chr1_3:67936430-67936449 None:intergenic 25.0%
! AACACTATAAGTGCTAAAAT+TGG + chr1_3:67936520-67936539 None:intergenic 25.0%
! AAAAATCAGGAAAAAGATCT+AGG - chr1_3:67936637-67936656 Msa0136140:intron 25.0%
! TTCTTATACATAATACCAGA+GGG + chr1_3:67936714-67936733 None:intergenic 25.0%
! TTTCTTATACATAATACCAG+AGG + chr1_3:67936715-67936734 None:intergenic 25.0%
!!! TCAATGTGTTTAATTTTGTC+TGG - chr1_3:67936880-67936899 Msa0136140:CDS 25.0%
!! TTCAACAGATTTGAAGTTAA+AGG - chr1_3:67937124-67937143 Msa0136140:intron 25.0%
!! TCAACAGATTTGAAGTTAAA+GGG - chr1_3:67937125-67937144 Msa0136140:intron 25.0%
! AAGGGAAAATCAAAATGTAA+TGG - chr1_3:67937143-67937162 Msa0136140:intron 25.0%
!!! GTATTTTTAGGAAATGCAAT+TGG + chr1_3:67937215-67937234 None:intergenic 25.0%
! GGACATAAAATTATTAGCAA+TGG + chr1_3:67937237-67937256 None:intergenic 25.0%
! AATTCAAAATCACAGTACTT+TGG + chr1_3:67937272-67937291 None:intergenic 25.0%
CTTGGAAGTTATTAAGTAAC+TGG + chr1_3:67936251-67936270 None:intergenic 30.0%
TAGAAATTCCATCTACATCA+TGG - chr1_3:67936301-67936320 Msa0136140:intron 30.0%
TTTAGCACTTATAGTGTTCA+AGG - chr1_3:67936522-67936541 Msa0136140:intron 30.0%
! AAGTGTTTTCAGTTATCTGT+TGG + chr1_3:67936557-67936576 None:intergenic 30.0%
! AGAGTTTGGTAAAGATGTTT+TGG - chr1_3:67936581-67936600 Msa0136140:CDS 30.0%
! ATCTTTTTCCTGATTTTTCC+AGG + chr1_3:67936635-67936654 None:intergenic 30.0%
ACAAGAGAAATGTGAAATCT+AGG - chr1_3:67936955-67936974 Msa0136140:CDS 30.0%
TCAAATCTGTTGAATCATTC+TGG + chr1_3:67937118-67937137 None:intergenic 30.0%
GGAAAATCAAAATGTAATGG+AGG - chr1_3:67937146-67937165 Msa0136140:intron 30.0%
GTACTTTGGACAAAAATTCA+AGG + chr1_3:67937258-67937277 None:intergenic 30.0%
TCTTACTCATGAAATATAGG+TGG - chr1_3:67937470-67937489 Msa0136140:CDS 30.0%
! GCAATATGTGAAGTTTTTAC+AGG - chr1_3:67937521-67937540 Msa0136140:CDS 30.0%
! GTTTTTACAGGTCCAAATTA+AGG - chr1_3:67937533-67937552 Msa0136140:CDS 30.0%
TTCATTCCATTGTAAGCAAT+TGG + chr1_3:67937736-67937755 None:intergenic 30.0%
CTTGGAAGTTATTAAGTAAC+TGG + chr1_3:67936251-67936270 None:intergenic 30.0%
TAGAAATTCCATCTACATCA+TGG - chr1_3:67936301-67936320 Msa0136140:intron 30.0%
TTTAGCACTTATAGTGTTCA+AGG - chr1_3:67936522-67936541 Msa0136140:intron 30.0%
! AAGTGTTTTCAGTTATCTGT+TGG + chr1_3:67936557-67936576 None:intergenic 30.0%
! AGAGTTTGGTAAAGATGTTT+TGG - chr1_3:67936581-67936600 Msa0136140:CDS 30.0%
! ATCTTTTTCCTGATTTTTCC+AGG + chr1_3:67936635-67936654 None:intergenic 30.0%
ACAAGAGAAATGTGAAATCT+AGG - chr1_3:67936955-67936974 Msa0136140:CDS 30.0%
TCAAATCTGTTGAATCATTC+TGG + chr1_3:67937118-67937137 None:intergenic 30.0%
GGAAAATCAAAATGTAATGG+AGG - chr1_3:67937146-67937165 Msa0136140:intron 30.0%
GTACTTTGGACAAAAATTCA+AGG + chr1_3:67937258-67937277 None:intergenic 30.0%
TCTTACTCATGAAATATAGG+TGG - chr1_3:67937470-67937489 Msa0136140:CDS 30.0%
! GCAATATGTGAAGTTTTTAC+AGG - chr1_3:67937521-67937540 Msa0136140:CDS 30.0%
! GTTTTTACAGGTCCAAATTA+AGG - chr1_3:67937533-67937552 Msa0136140:CDS 30.0%
TTCATTCCATTGTAAGCAAT+TGG + chr1_3:67937736-67937755 None:intergenic 30.0%
AATGATCATGTAGCCAACAT+TGG - chr1_3:67936483-67936502 Msa0136140:intron 35.0%
! GAAAACACTTCTGAAGAGTT+TGG - chr1_3:67936567-67936586 Msa0136140:CDS 35.0%
AAGAACAACCTGGAAAAATC+AGG - chr1_3:67936624-67936643 Msa0136140:intron 35.0%
TCTTTGTAAATTACTCCCTC+TGG - chr1_3:67936696-67936715 Msa0136140:intron 35.0%
! TTGTTTACATTTAAGACCGG+AGG - chr1_3:67936782-67936801 Msa0136140:CDS 35.0%
! TGTTTACATTTAAGACCGGA+GGG - chr1_3:67936783-67936802 Msa0136140:CDS 35.0%
!!! TCCTGCTTTTTTTATAGTCC+TGG - chr1_3:67936817-67936836 Msa0136140:CDS 35.0%
ACCAGGACTATAAAAAAAGC+AGG + chr1_3:67936821-67936840 None:intergenic 35.0%
GTGATATTGTGACAAGCATT+TGG - chr1_3:67936839-67936858 Msa0136140:CDS 35.0%
! CTCTTGTTCTTTTGTACCTA+TGG + chr1_3:67936942-67936961 None:intergenic 35.0%
ATCTAGGAAGAAACCAAAGT+CGG - chr1_3:67936971-67936990 Msa0136140:CDS 35.0%
!! GCTTTAAAGAAGCTAAATGC+AGG - chr1_3:67937074-67937093 Msa0136140:intron 35.0%
ATGATTCATATCAAGGCCTA+AGG + chr1_3:67937584-67937603 None:intergenic 35.0%
TATGCTCCAATTGCTTACAA+TGG - chr1_3:67937727-67937746 Msa0136140:CDS 35.0%
GCTTACAATGGAATGAACAT+TGG - chr1_3:67937739-67937758 Msa0136140:CDS 35.0%
AATGATCATGTAGCCAACAT+TGG - chr1_3:67936483-67936502 Msa0136140:intron 35.0%
! GAAAACACTTCTGAAGAGTT+TGG - chr1_3:67936567-67936586 Msa0136140:CDS 35.0%
AAGAACAACCTGGAAAAATC+AGG - chr1_3:67936624-67936643 Msa0136140:intron 35.0%
TCTTTGTAAATTACTCCCTC+TGG - chr1_3:67936696-67936715 Msa0136140:intron 35.0%
! TTGTTTACATTTAAGACCGG+AGG - chr1_3:67936782-67936801 Msa0136140:CDS 35.0%
! TGTTTACATTTAAGACCGGA+GGG - chr1_3:67936783-67936802 Msa0136140:CDS 35.0%
!!! TCCTGCTTTTTTTATAGTCC+TGG - chr1_3:67936817-67936836 Msa0136140:CDS 35.0%
ACCAGGACTATAAAAAAAGC+AGG + chr1_3:67936821-67936840 None:intergenic 35.0%
GTGATATTGTGACAAGCATT+TGG - chr1_3:67936839-67936858 Msa0136140:CDS 35.0%
! CTCTTGTTCTTTTGTACCTA+TGG + chr1_3:67936942-67936961 None:intergenic 35.0%
ATCTAGGAAGAAACCAAAGT+CGG - chr1_3:67936971-67936990 Msa0136140:CDS 35.0%
!! GCTTTAAAGAAGCTAAATGC+AGG - chr1_3:67937074-67937093 Msa0136140:intron 35.0%
ATGATTCATATCAAGGCCTA+AGG + chr1_3:67937584-67937603 None:intergenic 35.0%
TATGCTCCAATTGCTTACAA+TGG - chr1_3:67937727-67937746 Msa0136140:CDS 35.0%
GCTTACAATGGAATGAACAT+TGG - chr1_3:67937739-67937758 Msa0136140:CDS 35.0%
GTGAGCAAAGTTGTGAAGAA+TGG - chr1_3:67936178-67936197 Msa0136140:CDS 40.0%
GAAGAATGGAGTTCTCTTAG+TGG - chr1_3:67936192-67936211 Msa0136140:CDS 40.0%
!!! TAGTGGATTTTACACAGCTG+AGG - chr1_3:67936209-67936228 Msa0136140:CDS 40.0%
!! AGCTGAGGAAGCTGATTTTA+TGG - chr1_3:67936224-67936243 Msa0136140:CDS 40.0%
!! GCTGAGGAAGCTGATTTTAT+GGG - chr1_3:67936225-67936244 Msa0136140:CDS 40.0%
CATAATGGTGCTCAGAAACT+TGG + chr1_3:67936269-67936288 None:intergenic 40.0%
CAAGTTTCTGAGCACCATTA+TGG - chr1_3:67936267-67936286 Msa0136140:intron 40.0%
ACTATTGTGGATTCATGTCC+TGG + chr1_3:67936327-67936346 None:intergenic 40.0%
GCTATTCATGCTCACTATTG+TGG + chr1_3:67936340-67936359 None:intergenic 40.0%
GTTCTTGATACACTCTTTCG+CGG + chr1_3:67936610-67936629 None:intergenic 40.0%
AGAGTGTATCAAGAACAACC+TGG - chr1_3:67936614-67936633 Msa0136140:intron 40.0%
! AAGAAAAAAGGTGCGTGTGT+TGG - chr1_3:67936729-67936748 Msa0136140:intron 40.0%
ATGCTTGTCACAATATCACC+AGG + chr1_3:67936838-67936857 None:intergenic 40.0%
!! TTGCTTTTGAACTCCGACTT+TGG + chr1_3:67936987-67937006 None:intergenic 40.0%
GGATGAAAGTCATGATCTTC+AGG - chr1_3:67937016-67937035 Msa0136140:intron 40.0%
! ACTTTTGCATAGACACCTTG+TGG + chr1_3:67937185-67937204 None:intergenic 40.0%
AAGCTACCTTGGTTCCATTA+GGG + chr1_3:67937390-67937409 None:intergenic 40.0%
AAAGCTACCTTGGTTCCATT+AGG + chr1_3:67937391-67937410 None:intergenic 40.0%
TGTCATTGAGAAAGCTACCT+TGG + chr1_3:67937401-67937420 None:intergenic 40.0%
ATTCATATCAAGGCCTAAGG+AGG + chr1_3:67937581-67937600 None:intergenic 40.0%
GTGAGCAAAGTTGTGAAGAA+TGG - chr1_3:67936178-67936197 Msa0136140:CDS 40.0%
GAAGAATGGAGTTCTCTTAG+TGG - chr1_3:67936192-67936211 Msa0136140:CDS 40.0%
!!! TAGTGGATTTTACACAGCTG+AGG - chr1_3:67936209-67936228 Msa0136140:CDS 40.0%
!! AGCTGAGGAAGCTGATTTTA+TGG - chr1_3:67936224-67936243 Msa0136140:CDS 40.0%
!! GCTGAGGAAGCTGATTTTAT+GGG - chr1_3:67936225-67936244 Msa0136140:CDS 40.0%
CATAATGGTGCTCAGAAACT+TGG + chr1_3:67936269-67936288 None:intergenic 40.0%
CAAGTTTCTGAGCACCATTA+TGG - chr1_3:67936267-67936286 Msa0136140:intron 40.0%
ACTATTGTGGATTCATGTCC+TGG + chr1_3:67936327-67936346 None:intergenic 40.0%
GCTATTCATGCTCACTATTG+TGG + chr1_3:67936340-67936359 None:intergenic 40.0%
GTTCTTGATACACTCTTTCG+CGG + chr1_3:67936610-67936629 None:intergenic 40.0%
AGAGTGTATCAAGAACAACC+TGG - chr1_3:67936614-67936633 Msa0136140:intron 40.0%
! AAGAAAAAAGGTGCGTGTGT+TGG - chr1_3:67936729-67936748 Msa0136140:intron 40.0%
ATGCTTGTCACAATATCACC+AGG + chr1_3:67936838-67936857 None:intergenic 40.0%
!! TTGCTTTTGAACTCCGACTT+TGG + chr1_3:67936987-67937006 None:intergenic 40.0%
GGATGAAAGTCATGATCTTC+AGG - chr1_3:67937016-67937035 Msa0136140:intron 40.0%
! ACTTTTGCATAGACACCTTG+TGG + chr1_3:67937185-67937204 None:intergenic 40.0%
AAGCTACCTTGGTTCCATTA+GGG + chr1_3:67937390-67937409 None:intergenic 40.0%
AAAGCTACCTTGGTTCCATT+AGG + chr1_3:67937391-67937410 None:intergenic 40.0%
TGTCATTGAGAAAGCTACCT+TGG + chr1_3:67937401-67937420 None:intergenic 40.0%
ATTCATATCAAGGCCTAAGG+AGG + chr1_3:67937581-67937600 None:intergenic 40.0%
ATTCCATCTACATCATGGCC+AGG - chr1_3:67936306-67936325 Msa0136140:intron 45.0%
GGCATTCATGTAGCCAATGT+TGG + chr1_3:67936499-67936518 None:intergenic 45.0%
AAGATCTAGGAGCTCGATGA+AGG - chr1_3:67936650-67936669 Msa0136140:intron 45.0%
TCAGGAGCAGAATTTGAGCT+CGG - chr1_3:67937034-67937053 Msa0136140:intron 45.0%
ATAGACACCTTGTGGATCAG+AGG + chr1_3:67937177-67937196 None:intergenic 45.0%
AAGGATCCTCATTCCTCCTT+AGG - chr1_3:67937565-67937584 Msa0136140:CDS 45.0%
! TTCTGAGCTCTGATGATCAC+TGG - chr1_3:67937701-67937720 Msa0136140:CDS 45.0%
GTTCGGTTGGGATAATGTTG+AGG + chr1_3:67937771-67937790 None:intergenic 45.0%
ATTCCATCTACATCATGGCC+AGG - chr1_3:67936306-67936325 Msa0136140:intron 45.0%
GGCATTCATGTAGCCAATGT+TGG + chr1_3:67936499-67936518 None:intergenic 45.0%
AAGATCTAGGAGCTCGATGA+AGG - chr1_3:67936650-67936669 Msa0136140:intron 45.0%
TCAGGAGCAGAATTTGAGCT+CGG - chr1_3:67937034-67937053 Msa0136140:intron 45.0%
ATAGACACCTTGTGGATCAG+AGG + chr1_3:67937177-67937196 None:intergenic 45.0%
AAGGATCCTCATTCCTCCTT+AGG - chr1_3:67937565-67937584 Msa0136140:CDS 45.0%
! TTCTGAGCTCTGATGATCAC+TGG - chr1_3:67937701-67937720 Msa0136140:CDS 45.0%
GTTCGGTTGGGATAATGTTG+AGG + chr1_3:67937771-67937790 None:intergenic 45.0%
TGTCCTGGCCATGATGTAGA+TGG + chr1_3:67936312-67936331 None:intergenic 50.0%
TCTAGGAGCTCGATGAAGGT+AGG - chr1_3:67936654-67936673 Msa0136140:intron 50.0%
AGGAAGTGACTTACTCCCTC+CGG + chr1_3:67936801-67936820 None:intergenic 50.0%
GTTCAAAAGCAACGCTGACG+AGG - chr1_3:67936995-67937014 Msa0136140:CDS 50.0%
CATTAGGGACTAGGCTTTGC+AGG + chr1_3:67937375-67937394 None:intergenic 50.0%
CTGCAAAGCCTAGTCCCTAA+TGG - chr1_3:67937373-67937392 Msa0136140:CDS 50.0%
CCTTGGTTCCATTAGGGACT+AGG + chr1_3:67937384-67937403 None:intergenic 50.0%
CCTAGTCCCTAATGGAACCA+AGG - chr1_3:67937381-67937400 Msa0136140:CDS 50.0%
GGATATCAGCACCATGCTTG+AGG - chr1_3:67937491-67937510 Msa0136140:CDS 50.0%
TTGCACAGCTTCCTCAAGCA+TGG + chr1_3:67937505-67937524 None:intergenic 50.0%
TCAAGGCCTAAGGAGGAATG+AGG + chr1_3:67937574-67937593 None:intergenic 50.0%
TGTCCTGGCCATGATGTAGA+TGG + chr1_3:67936312-67936331 None:intergenic 50.0%
TCTAGGAGCTCGATGAAGGT+AGG - chr1_3:67936654-67936673 Msa0136140:intron 50.0%
AGGAAGTGACTTACTCCCTC+CGG + chr1_3:67936801-67936820 None:intergenic 50.0%
GTTCAAAAGCAACGCTGACG+AGG - chr1_3:67936995-67937014 Msa0136140:CDS 50.0%
CATTAGGGACTAGGCTTTGC+AGG + chr1_3:67937375-67937394 None:intergenic 50.0%
CTGCAAAGCCTAGTCCCTAA+TGG - chr1_3:67937373-67937392 Msa0136140:CDS 50.0%
CCTTGGTTCCATTAGGGACT+AGG + chr1_3:67937384-67937403 None:intergenic 50.0%
CCTAGTCCCTAATGGAACCA+AGG - chr1_3:67937381-67937400 Msa0136140:CDS 50.0%
GGATATCAGCACCATGCTTG+AGG - chr1_3:67937491-67937510 Msa0136140:CDS 50.0%
TTGCACAGCTTCCTCAAGCA+TGG + chr1_3:67937505-67937524 None:intergenic 50.0%
TCAAGGCCTAAGGAGGAATG+AGG + chr1_3:67937574-67937593 None:intergenic 50.0%
GGAGCAGAATTTGAGCTCGG+AGG - chr1_3:67937037-67937056 Msa0136140:intron 55.0%
GGTTCTGCCTCTGATCCACA+AGG - chr1_3:67937167-67937186 Msa0136140:intron 55.0%
GGAGCAGAATTTGAGCTCGG+AGG - chr1_3:67937037-67937056 Msa0136140:intron 55.0%
GGTTCTGCCTCTGATCCACA+AGG - chr1_3:67937167-67937186 Msa0136140:intron 55.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 67936168 67937798 67936168 ID=Msa0136140;Name=Msa0136140
chr1_3 mRNA 67936168 67937798 67936168 ID=Msa0136140-mRNA-1;Parent=Msa0136140;Name=Msa0136140-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|0|0|1|1|1|5|0|330
chr1_3 exon 67937295 67937798 67937295 ID=Msa0136140-mRNA-1:exon:23438;Parent=Msa0136140-mRNA-1
chr1_3 exon 67936764 67937021 67936764 ID=Msa0136140-mRNA-1:exon:23437;Parent=Msa0136140-mRNA-1
chr1_3 exon 67936564 67936629 67936564 ID=Msa0136140-mRNA-1:exon:23436;Parent=Msa0136140-mRNA-1
chr1_3 exon 67936412 67936477 67936412 ID=Msa0136140-mRNA-1:exon:23435;Parent=Msa0136140-mRNA-1
chr1_3 exon 67936168 67936266 67936168 ID=Msa0136140-mRNA-1:exon:23434;Parent=Msa0136140-mRNA-1
chr1_3 CDS 67937295 67937798 67937295 ID=Msa0136140-mRNA-1:cds;Parent=Msa0136140-mRNA-1
chr1_3 CDS 67936764 67937021 67936764 ID=Msa0136140-mRNA-1:cds;Parent=Msa0136140-mRNA-1
chr1_3 CDS 67936564 67936629 67936564 ID=Msa0136140-mRNA-1:cds;Parent=Msa0136140-mRNA-1
chr1_3 CDS 67936412 67936477 67936412 ID=Msa0136140-mRNA-1:cds;Parent=Msa0136140-mRNA-1
chr1_3 CDS 67936168 67936266 67936168 ID=Msa0136140-mRNA-1:cds;Parent=Msa0136140-mRNA-1
Gene Sequence

>Msa0136140

ATGGATTCTGGTGAGCAAAGTTGTGAAGAATGGAGTTCTCTTAGTGGATTTTACACAGCTGAGGAAGCTGATTTTATGGGCCAGTTACTTAATAACTTCCAAGTTTCTGAGCACCATTATGGAAATTTCAATTTAGAAATTCCATCTACATCATGGCCAGGACATGAATCCACAATAGTGAGCATGAATAGCAGTTCACATTTTCCTCAAAATGCTGATAATAGTAACACTGATGTTTTTAGTTTTTCACAAAGTAGCTCCATTTCTGATACTAGCAATAAATTTCATAATACAAATGGTGGTAACATCTGTTTGAATGATCATGTAGCCAACATTGGCTACATGAATGCCAATTTTAGCACTTATAGTGTTCAAGGTCATGACAGCCAACAGATAACTGAAAACACTTCTGAAGAGTTTGGTAAAGATGTTTTGGAATCCGCGAAAGAGTGTATCAAGAACAACCTGGAAAAATCAGGAAAAAGATCTAGGAGCTCGATGAAGGTACAAAAGAACAAGAGAAATGTGAAATCTAGGAAGAAACCAAAGTCGGAGTTCAAAAGCAACGCTGACGAGGATGAAAGTCATGATCTTCAGGAGCAGAATTTGAGCTCGGAGGATGATGATATCAATGCTTTAAAGAAGCTAAATGCAGGAACATCTTCAATCTTGAACCAGAATGATTCAACAGATTTGAAGTTAAAGGGAAAATCAAAATGTAATGGAGGTTCTGCCTCTGATCCACAAGGTGTCTATGCAAAAAAAAGAAGAGAAAGAATAAATGAAAGATTGAAAATCCTGCAAAGCCTAGTCCCTAATGGAACCAAGGTGGATATCAGCACCATGCTTGAGGAAGCTGTGCAATATGTGAAGTTTTTACAGGTCCAAATTAAGCTTCTGAGCTCTGATGATCACTGGATGTATGCTCCAATTGCTTACAATGGAATGAACATTGGACTAAACCTCAACATTATCCCAACCGAACTACCATAG
Protein sequence

>Msa0136140

MDSGEQSCEEWSSLSGFYTAEEADFMGQLLNNFQVSEHHYGNFNLEIPSTSWPGHESTIVSMNSSSHFPQNADNSNTDVFSFSQSSSISDTSNKFHNTNGGNICLNDHVANIGYMNANFSTYSVQGHDSQQITENTSEEFGKDVLESAKECIKNNLEKSGKRSRSSMKVQKNKRNVKSRKKPKSEFKSNADEDESHDLQEQNLSSEDDDINALKKLNAGTSSILNQNDSTDLKLKGKSKCNGGSASDPQGVYAKKRRERINERLKILQSLVPNGTKVDISTMLEEAVQYVKFLQVQIKLLSSDDHWMYAPIAYNGMNIGLNLNIIPTELP*