AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0137110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0137110 MtrunA17_Chr5g0409501 72.093 43 11 1 1 43 1 42 6.38e-14 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 69 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTGACAGGCTGCTGTGTT+TGG 0.226175 1_3:-69416861 Msa0137110:three_prime_UTR
TGCTGCTGTTTGGGTTCATT+AGG 0.259959 1_3:-69416908 Msa0137110:three_prime_UTR
TTTGACAGGCTGCTGTGTTT+GGG 0.260405 1_3:-69416860 Msa0137110:three_prime_UTR
ATATACCTATTGTGTATAAT+TGG 0.278386 1_3:+69418190 None:intergenic
GACTGTTTCGTGCTGCTGTT+TGG 0.303173 1_3:-69416918 Msa0137110:three_prime_UTR
TTGTGAGGCGGAGGTATTCT+AGG 0.323878 1_3:-69416692 Msa0137110:three_prime_UTR
GCTGCTGTTTGGGTTCATTA+GGG 0.329201 1_3:-69416907 Msa0137110:three_prime_UTR
TGTTCCTTTCCGCGAGTTGT+TGG 0.365499 1_3:-69416584 Msa0137110:three_prime_UTR
GCAGGTGCTGCTGTTGGTGT+TGG 0.374496 1_3:-69416830 Msa0137110:three_prime_UTR
TATCATGCAGACTCTCTTTG+AGG 0.377142 1_3:-69418270 Msa0137110:intron
TTTCCCCTCCTAGCCTTTGC+GGG 0.390956 1_3:-69416722 Msa0137110:three_prime_UTR
CAGGTGCTGCTGTTGGTGTT+GGG 0.414089 1_3:-69416829 Msa0137110:three_prime_UTR
ACTGTTTCGTGCTGCTGTTT+GGG 0.416253 1_3:-69416917 Msa0137110:three_prime_UTR
CTTTCCCCTCCTAGCCTTTG+CGG 0.419409 1_3:-69416723 Msa0137110:three_prime_UTR
TTGAAGAGTGTATACTATCT+TGG 0.421203 1_3:+69417513 None:intergenic
TTGAAGTTTCTTCTAAGTGT+TGG 0.425711 1_3:-69417608 Msa0137110:three_prime_UTR
TAGTACCTTGGTACATTGCT+TGG 0.447221 1_3:-69416664 Msa0137110:three_prime_UTR
AGCCTTCAATATACCTTGGT+TGG 0.451057 1_3:-69416622 Msa0137110:three_prime_UTR
GCCTTCAATATACCTTGGTT+GGG 0.452692 1_3:-69416621 Msa0137110:three_prime_UTR
TTAAACTTACTTTATAACAT+TGG 0.467968 1_3:-69416378 Msa0137110:three_prime_UTR
GTGTTGGCAGGTGCTGCTGT+TGG 0.471847 1_3:-69416836 Msa0137110:three_prime_UTR
TTAGTCTGTTTCGTGCTGCT+TGG 0.472547 1_3:-69416947 Msa0137110:three_prime_UTR
TGGTTCCAATTATACACAAT+AGG 0.473155 1_3:-69418195 Msa0137110:intron
TGTTGGGTTGCTGTCGTAGC+AGG 0.476983 1_3:-69416813 Msa0137110:intron
AACATGTTTAAATAATGTCC+TGG 0.490974 1_3:+69419195 None:intergenic
TTGACAGGCTGCTGTGTTTG+GGG 0.493111 1_3:-69416859 Msa0137110:three_prime_UTR
ACCTTCCTTTGCACTAATGT+AGG 0.502129 1_3:+69418225 None:intergenic
TGGCAGCCTTCAATATACCT+TGG 0.503384 1_3:-69416626 Msa0137110:three_prime_UTR
CCTCTGCCGCACCAGAAAAG+TGG 0.510030 1_3:-69419247 Msa0137110:CDS
TGACAGGCTGCTGTGTTTGG+GGG 0.513369 1_3:-69416858 Msa0137110:three_prime_UTR
CCTTGGTACATTGCTTGGAG+TGG 0.522618 1_3:-69416659 Msa0137110:three_prime_UTR
CTGTTTGGGTTCATTAGGGG+TGG 0.527027 1_3:-69416903 Msa0137110:three_prime_UTR
GCCCAACCAAGGTATATTGA+AGG 0.529112 1_3:+69416620 Msa0137100:CDS
TGTGAGGCGGAGGTATTCTA+GGG 0.534048 1_3:-69416691 Msa0137110:three_prime_UTR
TCATAGCTTGCTCCTCATCA+AGG 0.540953 1_3:+69417005 None:intergenic
ATCTGCTACCCGCAAAGGCT+AGG 0.542672 1_3:+69416714 Msa0137100:CDS
CCGATTGCAAAATAAACCTA+GGG 0.543286 1_3:+69419305 None:intergenic
GCTACCCGCAAAGGCTAGGA+GGG 0.545225 1_3:+69416718 Msa0137100:CDS
TTCTAGGGGTGGTAGTACCT+TGG 0.546841 1_3:-69416676 Msa0137110:three_prime_UTR
ATATAATTGACGATGTTCAC+AGG 0.552222 1_3:-69417583 Msa0137110:intron
AACACCAACAACTCGCGGAA+AGG 0.562210 1_3:+69416580 Msa0137100:CDS
TGGGTTCATTAGGGGTGGCG+TGG 0.568705 1_3:-69416898 Msa0137110:three_prime_UTR
TATTGCCTACATTAGTGCAA+AGG 0.571128 1_3:-69418230 Msa0137110:CDS
ATAAGTAGAAATATTTGTAG+TGG 0.574416 1_3:-69416490 Msa0137110:three_prime_UTR
GGTAGCAGATTGTGAGGCGG+AGG 0.577700 1_3:-69416701 Msa0137110:three_prime_UTR
CAATCGGAGCAACTTTGTCG+AGG 0.582928 1_3:-69419289 Msa0137110:CDS
TGCTACCCGCAAAGGCTAGG+AGG 0.584137 1_3:+69416717 Msa0137100:CDS
CTGCTGTTTGGGTTCATTAG+GGG 0.584859 1_3:-69416906 Msa0137110:three_prime_UTR
TTTGTCGAGGACCACCGTGC+CGG 0.586060 1_3:-69419276 Msa0137110:CDS
TCACAATCTGCTACCCGCAA+AGG 0.602076 1_3:+69416709 Msa0137100:CDS
TCCGATTGCAAAATAAACCT+AGG 0.611275 1_3:+69419304 None:intergenic
TTTGCGGGTAGCAGATTGTG+AGG 0.613528 1_3:-69416707 Msa0137110:three_prime_UTR
GGTGCGGCAGAGGTGAACTC+CGG 0.614603 1_3:+69419257 None:intergenic
GCCTACATTAGTGCAAAGGA+AGG 0.622054 1_3:-69418226 Msa0137110:CDS
ATATCAAATTGCAGTGAGGG+AGG 0.624092 1_3:-69417675 Msa0137110:intron
TGCAAAGGAAGGTGACACAC+TGG 0.626735 1_3:-69418215 Msa0137110:CDS
CTACCCGCAAAGGCTAGGAG+GGG 0.632710 1_3:+69416719 Msa0137100:CDS
ATGAGGAGCAAGCTATGATG+AGG 0.633367 1_3:-69417000 Msa0137110:three_prime_UTR
ATGTCAGCAACAAAACCCCT+AGG 0.637382 1_3:-69419321 Msa0137110:CDS
AAAGAAACACCAACAACTCG+CGG 0.660550 1_3:+69416575 Msa0137100:CDS
AATACATAAATGCCCAACCA+AGG 0.661177 1_3:+69416609 Msa0137100:CDS
GCGGGTAGCAGATTGTGAGG+CGG 0.663830 1_3:-69416704 Msa0137110:three_prime_UTR
AGGCGGAGGTATTCTAGGGG+TGG 0.666216 1_3:-69416687 Msa0137110:three_prime_UTR
GGCAGAGGTGAACTCCGGCA+CGG 0.666645 1_3:+69419262 None:intergenic
CGATTGCAAAATAAACCTAG+GGG 0.679356 1_3:+69419306 None:intergenic
TTATGGTTGAATCCTTGATG+AGG 0.693140 1_3:-69417017 Msa0137110:three_prime_UTR
CCACTCCAAGCAATGTACCA+AGG 0.709941 1_3:+69416659 Msa0137100:CDS
GTGAGGCGGAGGTATTCTAG+GGG 0.741583 1_3:-69416690 Msa0137110:three_prime_UTR
AGAGGTGAACTCCGGCACGG+TGG 0.742299 1_3:+69419265 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAACTAAAATATTGAAA+AGG + chr1_3:69417080-69417099 None:intergenic 10.0%
!! ATTAGCTATTTAAAAAAAAA+TGG - chr1_3:69419147-69419166 Msa0137110:intron 10.0%
!! TTAGCTATTTAAAAAAAAAT+GGG - chr1_3:69419148-69419167 Msa0137110:intron 10.0%
!!! ACATTATTTAAACATGTTTT+TGG - chr1_3:69416487-69416506 Msa0137110:three_prime_UTR 15.0%
!! AAAAAAGTAAAGTAACTAAA+TGG + chr1_3:69417911-69417930 None:intergenic 15.0%
!!! TTCTGAATTTGTTTTTATTT+TGG - chr1_3:69418228-69418247 Msa0137110:CDS 15.0%
!! TTAAACTTACTTTATAACAT+TGG - chr1_3:69419299-69419318 Msa0137110:CDS 15.0%
!!! TGATTAATTTTGAGAGATTT+GGG - chr1_3:69416683-69416702 Msa0137110:three_prime_UTR 20.0%
!! ATTAAAAACTGATAGAGAAA+TGG + chr1_3:69416741-69416760 Msa0137100:CDS 20.0%
!! GCAACAAATAAAAAATACTA+AGG + chr1_3:69417111-69417130 None:intergenic 20.0%
!! AAAAAAAGTAGAGTAGATTA+AGG + chr1_3:69417388-69417407 None:intergenic 20.0%
!! ATATACCTATTGTGTATAAT+TGG + chr1_3:69417490-69417509 None:intergenic 20.0%
!!! TTTTGATAGAGAAGTTAATT+TGG - chr1_3:69417544-69417563 Msa0137110:intron 20.0%
!! GATAATTATGTTAACTGAAT+TGG - chr1_3:69417710-69417729 Msa0137110:intron 20.0%
!! AATATATAGAATGTAATAGC+AGG - chr1_3:69417744-69417763 Msa0137110:intron 20.0%
!! AGTACAATACAATAACAATT+AGG + chr1_3:69417823-69417842 None:intergenic 20.0%
!! AAAATACTATTTGCAAACAA+AGG + chr1_3:69417854-69417873 None:intergenic 20.0%
!! ATTCAATGACATATATACTA+TGG + chr1_3:69418555-69418574 None:intergenic 20.0%
!! AGTATATATGTCATTGAATT+TGG - chr1_3:69418556-69418575 Msa0137110:intron 20.0%
!! ATAAGTAGAAATATTTGTAG+TGG - chr1_3:69419187-69419206 Msa0137110:intron 20.0%
!!! AACTTTTTTTTTAATTGAGC+TGG - chr1_3:69419258-69419277 Msa0137110:CDS 20.0%
! AACATGTTTAAATAATGTCC+TGG + chr1_3:69416485-69416504 Msa0137100:intron 25.0%
! CCTTAATATCTAATCTATCA+TGG + chr1_3:69416643-69416662 Msa0137100:CDS 25.0%
! CCATGATAGATTAGATATTA+AGG - chr1_3:69416640-69416659 Msa0137110:three_prime_UTR 25.0%
! ATATTAAGGAATTTAAGTCC+TGG - chr1_3:69416654-69416673 Msa0137110:three_prime_UTR 25.0%
! TATTAAGGAATTTAAGTCCT+GGG - chr1_3:69416655-69416674 Msa0137110:three_prime_UTR 25.0%
!!! GTGATTAATTTTGAGAGATT+TGG - chr1_3:69416682-69416701 Msa0137110:three_prime_UTR 25.0%
! CCTGAGAATTAAACAAATAA+TGG + chr1_3:69416800-69416819 Msa0137100:CDS 25.0%
! CCATTATTTGTTTAATTCTC+AGG - chr1_3:69416797-69416816 Msa0137110:intron 25.0%
!!! TGATTTGTTAAAATTGCTCT+TGG - chr1_3:69417679-69417698 Msa0137110:intron 25.0%
! ATGGAAGAAGAAAAAAAACT+GGG + chr1_3:69417941-69417960 None:intergenic 25.0%
! AATGGAAGAAGAAAAAAAAC+TGG + chr1_3:69417942-69417961 None:intergenic 25.0%
! GAAGTCTTCAAATTCATAAA+TGG + chr1_3:69417960-69417979 None:intergenic 25.0%
! TTAATACTAATGCAATCCTA+GGG + chr1_3:69418446-69418465 None:intergenic 25.0%
!!! TTTACATTTCTAGAACTCAA+AGG + chr1_3:69418469-69418488 None:intergenic 25.0%
! GATAGAGATAATCTGTTTAA+AGG - chr1_3:69418508-69418527 Msa0137110:intron 25.0%
!!! AATATTATTTTTCGTCGAGA+GGG + chr1_3:69419130-69419149 None:intergenic 25.0%
!!! TAATATTATTTTTCGTCGAG+AGG + chr1_3:69419131-69419150 None:intergenic 25.0%
! CATTTTCGAATTTTTGAACC+AGG - chr1_3:69416464-69416483 Msa0137110:three_prime_UTR 30.0%
!!! ATTCTCTGTTTTGATGTTCA+CGG - chr1_3:69416516-69416535 Msa0137110:three_prime_UTR 30.0%
AAAATCCCTAATCTCTCAAT+GGG + chr1_3:69416583-69416602 Msa0137100:CDS 30.0%
TAAAATCCCTAATCTCTCAA+TGG + chr1_3:69416584-69416603 Msa0137100:CDS 30.0%
AGTAAGATTAGAGAGAGATT+AGG - chr1_3:69416615-69416634 Msa0137110:three_prime_UTR 30.0%
GTAAGATTAGAGAGAGATTA+GGG - chr1_3:69416616-69416635 Msa0137110:three_prime_UTR 30.0%
!! TTTGAGAGATTTGGGATTTT+AGG - chr1_3:69416691-69416710 Msa0137110:three_prime_UTR 30.0%
!! TTGAGAGATTTGGGATTTTA+GGG - chr1_3:69416692-69416711 Msa0137110:three_prime_UTR 30.0%
TCATATCTCTCTAATGCATA+TGG - chr1_3:69416844-69416863 Msa0137110:three_prime_UTR 30.0%
ATGTGGTGAAAATAACTTCA+AGG - chr1_3:69416909-69416928 Msa0137110:three_prime_UTR 30.0%
TGTGATTCTAATAGATACAG+TGG - chr1_3:69416959-69416978 Msa0137110:three_prime_UTR 30.0%
AGGTTACTAACCTTCAAAAA+GGG + chr1_3:69417060-69417079 None:intergenic 30.0%
AAGGTTACTAACCTTCAAAA+AGG + chr1_3:69417061-69417080 None:intergenic 30.0%
AAGTTGTATGTGATGAATGT+TGG - chr1_3:69417286-69417305 Msa0137110:intron 30.0%
TGGTTCCAATTATACACAAT+AGG - chr1_3:69417482-69417501 Msa0137110:intron 30.0%
TGCTGTCAACAATATTTGTA+TGG - chr1_3:69417580-69417599 Msa0137110:intron 30.0%
TGTCAACAATATTTGTATGG+AGG - chr1_3:69417583-69417602 Msa0137110:intron 30.0%
! GTTTTTCTGCTATATTCTGT+AGG - chr1_3:69417633-69417652 Msa0137110:CDS 30.0%
AAAATTGCTCTTGGAACAAT+TGG - chr1_3:69417688-69417707 Msa0137110:intron 30.0%
ATTTGCAAACAAAGGTCAAA+AGG + chr1_3:69417846-69417865 None:intergenic 30.0%
TCAAATATCAAATTGCAGTG+AGG - chr1_3:69417998-69418017 Msa0137110:intron 30.0%
CAAATATCAAATTGCAGTGA+GGG - chr1_3:69417999-69418018 Msa0137110:intron 30.0%
TTGAAGTTTCTTCTAAGTGT+TGG - chr1_3:69418069-69418088 Msa0137110:intron 30.0%
ATATAATTGACGATGTTCAC+AGG - chr1_3:69418094-69418113 Msa0137110:intron 30.0%
TATAAACCATCATTGTCATG+AGG + chr1_3:69418120-69418139 None:intergenic 30.0%
! TTGAAGAGTGTATACTATCT+TGG + chr1_3:69418167-69418186 None:intergenic 30.0%
CATAAGTTTCAACAATAACC+TGG + chr1_3:69418409-69418428 None:intergenic 30.0%
TGAGAATGTTATAATACCCT+AGG - chr1_3:69418427-69418446 Msa0137110:intron 30.0%
GTTAATACTAATGCAATCCT+AGG + chr1_3:69418447-69418466 None:intergenic 30.0%
! AGCATACTTTTCATTTTCAG+CGG - chr1_3:69418634-69418653 Msa0137110:intron 30.0%
CGATTGCAAAATAAACCTAG+GGG + chr1_3:69416374-69416393 Msa0137100:intron 35.0%
CCGATTGCAAAATAAACCTA+GGG + chr1_3:69416375-69416394 Msa0137100:intron 35.0%
!!! CCCTAGGTTTATTTTGCAAT+CGG - chr1_3:69416372-69416391 Msa0137110:three_prime_UTR 35.0%
TCCGATTGCAAAATAAACCT+AGG + chr1_3:69416376-69416395 Msa0137100:intron 35.0%
!! CGTATACAAAACCACTTTTC+TGG + chr1_3:69416444-69416463 Msa0137100:intron 35.0%
! TTTTGATGTTCACGGATGAA+GGG - chr1_3:69416524-69416543 Msa0137110:three_prime_UTR 35.0%
CAAAATTAATCACTGAACCC+AGG + chr1_3:69416675-69416694 Msa0137100:CDS 35.0%
TATCTCTCTAATGCATATGG+TGG - chr1_3:69416847-69416866 Msa0137110:three_prime_UTR 35.0%
ACTTGTTTACTGATGTGATG+TGG - chr1_3:69416892-69416911 Msa0137110:three_prime_UTR 35.0%
TAATAGATACAGTGGATAGC+AGG - chr1_3:69416967-69416986 Msa0137110:three_prime_UTR 35.0%
AATAGATACAGTGGATAGCA+GGG - chr1_3:69416968-69416987 Msa0137110:three_prime_UTR 35.0%
GGTTACTAACCTTCAAAAAG+GGG + chr1_3:69417059-69417078 None:intergenic 35.0%
!!! GTTGCTGAAAATTTTGAAGC+TGG - chr1_3:69417126-69417145 Msa0137110:intron 35.0%
!!! TGAAAATTTTGAAGCTGGCA+AGG - chr1_3:69417131-69417150 Msa0137110:intron 35.0%
! GGCAAGGTTGACTTATTAAT+TGG - chr1_3:69417147-69417166 Msa0137110:intron 35.0%
TAGCTGTATGTGATGAATGT+TGG - chr1_3:69417333-69417352 Msa0137110:intron 35.0%
TATTGCCTACATTAGTGCAA+AGG - chr1_3:69417447-69417466 Msa0137110:intron 35.0%
CAATAGGTATATCACTTCGT+TGG - chr1_3:69417498-69417517 Msa0137110:intron 35.0%
TTTCTGCTATATTCTGTAGG+CGG - chr1_3:69417636-69417655 Msa0137110:CDS 35.0%
CACAAATATTCCATCGACAT+TGG + chr1_3:69417776-69417795 None:intergenic 35.0%
CAATGTCGATGGAATATTTG+TGG - chr1_3:69417774-69417793 Msa0137110:intron 35.0%
TCTAATAGAGTAGAGCAACT+AGG + chr1_3:69418283-69418302 None:intergenic 35.0%
TGTGAGCACATCTATAGTTA+AGG + chr1_3:69418313-69418332 None:intergenic 35.0%
ATGTGCTCACATAAATCAAG+AGG - chr1_3:69418322-69418341 Msa0137110:intron 35.0%
CAAGAGGTTTATGCTTTGAT+AGG - chr1_3:69418338-69418357 Msa0137110:intron 35.0%
! TGCTTTTAGTATCAACATCC+AGG - chr1_3:69418388-69418407 Msa0137110:intron 35.0%
ATGCACAAAAACACAAGGTA+TGG + chr1_3:69418606-69418625 None:intergenic 35.0%
AACACATGCACAAAAACACA+AGG + chr1_3:69418611-69418630 None:intergenic 35.0%
!! TGTGTTTTTGTGCATGTGTT+AGG - chr1_3:69418611-69418630 Msa0137110:intron 35.0%
! ATACTTTTCATTTTCAGCGG+TGG - chr1_3:69418637-69418656 Msa0137110:intron 35.0%
TTATGGTTGAATCCTTGATG+AGG - chr1_3:69418660-69418679 Msa0137110:intron 35.0%
TGAGGAAAAACAAGCTTTCA+TGG - chr1_3:69418695-69418714 Msa0137110:intron 35.0%
GAGGAAAAACAAGCTTTCAT+GGG - chr1_3:69418696-69418715 Msa0137110:intron 35.0%
!!! CTTGGAGTGGTTTTTGTTTT+TGG - chr1_3:69419031-69419050 Msa0137110:intron 35.0%
AATACATAAATGCCCAACCA+AGG + chr1_3:69419071-69419090 None:intergenic 35.0%
!! ACAAAACCACTTTTCTGGTG+CGG + chr1_3:69416439-69416458 Msa0137100:intron 40.0%
!! GTTTTGATGTTCACGGATGA+AGG - chr1_3:69416523-69416542 Msa0137110:three_prime_UTR 40.0%
! TCTCAGGTGAAGTACTAAAG+AGG - chr1_3:69416813-69416832 Msa0137110:intron 40.0%
! CTCAGGTGAAGTACTAAAGA+GGG - chr1_3:69416814-69416833 Msa0137110:three_prime_UTR 40.0%
GCATTCCAAGAATCAAACTC+AGG + chr1_3:69417037-69417056 None:intergenic 40.0%
TATCATGCAGACTCTCTTTG+AGG - chr1_3:69417407-69417426 Msa0137110:intron 40.0%
ACCTTCCTTTGCACTAATGT+AGG + chr1_3:69417455-69417474 None:intergenic 40.0%
TTCTGCTATATTCTGTAGGC+GGG - chr1_3:69417637-69417656 Msa0137110:CDS 40.0%
ACAATTAGGCCCACACAAAT+AGG + chr1_3:69417809-69417828 None:intergenic 40.0%
ATATCAAATTGCAGTGAGGG+AGG - chr1_3:69418002-69418021 Msa0137110:intron 40.0%
! TTCATTTTCAGCGGTGGTTA+TGG - chr1_3:69418643-69418662 Msa0137110:intron 40.0%
!!! TCTGTTTTAGCAGGTTTGAC+AGG - chr1_3:69418803-69418822 Msa0137110:intron 40.0%
TAGTACCTTGGTACATTGCT+TGG - chr1_3:69419013-69419032 Msa0137110:intron 40.0%
AGCCTTCAATATACCTTGGT+TGG - chr1_3:69419055-69419074 Msa0137110:intron 40.0%
GCCTTCAATATACCTTGGTT+GGG - chr1_3:69419056-69419075 Msa0137110:intron 40.0%
AAAGAAACACCAACAACTCG+CGG + chr1_3:69419105-69419124 None:intergenic 40.0%
ATGTCAGCAACAAAACCCCT+AGG - chr1_3:69416356-69416375 Msa0137110:three_prime_UTR 45.0%
CCTAATCTCTCAATGGGTCA+CGG + chr1_3:69416577-69416596 Msa0137100:CDS 45.0%
CGTGACCCATTGAGAGATTA+GGG - chr1_3:69416575-69416594 Msa0137110:three_prime_UTR 45.0%
TAGTGGTGTGATTCTCGCTT+TGG - chr1_3:69417000-69417019 Msa0137110:three_prime_UTR 45.0%
AGTGGTGTGATTCTCGCTTT+GGG - chr1_3:69417001-69417020 Msa0137110:three_prime_UTR 45.0%
! ATTCTCGCTTTGGGTGTAAG+AGG - chr1_3:69417010-69417029 Msa0137110:three_prime_UTR 45.0%
GAGGTCCTGAGTTTGATTCT+TGG - chr1_3:69417029-69417048 Msa0137110:three_prime_UTR 45.0%
! CTTGGAATGCCCCTTTTTGA+AGG - chr1_3:69417047-69417066 Msa0137110:three_prime_UTR 45.0%
GCCTACATTAGTGCAAAGGA+AGG - chr1_3:69417451-69417470 Msa0137110:intron 45.0%
GATGCACTACTGCAACGATA+AGG + chr1_3:69417613-69417632 None:intergenic 45.0%
TCTGCTATATTCTGTAGGCG+GGG - chr1_3:69417638-69417657 Msa0137110:CDS 45.0%
GTCGATGGAATATTTGTGGC+TGG - chr1_3:69417778-69417797 Msa0137110:intron 45.0%
CTGGAAGCTCCTATTTGTGT+GGG - chr1_3:69417797-69417816 Msa0137110:intron 45.0%
!! TCATAGCTTGCTCCTCATCA+AGG + chr1_3:69418675-69418694 None:intergenic 45.0%
ATGAGGAGCAAGCTATGATG+AGG - chr1_3:69418677-69418696 Msa0137110:intron 45.0%
! TTAGTCTGTTTCGTGCTGCT+TGG - chr1_3:69418730-69418749 Msa0137110:intron 45.0%
! ACTGTTTCGTGCTGCTGTTT+GGG - chr1_3:69418760-69418779 Msa0137110:intron 45.0%
! TGCTGCTGTTTGGGTTCATT+AGG - chr1_3:69418769-69418788 Msa0137110:intron 45.0%
! GCTGCTGTTTGGGTTCATTA+GGG - chr1_3:69418770-69418789 Msa0137110:intron 45.0%
! CTGCTGTTTGGGTTCATTAG+GGG - chr1_3:69418771-69418790 Msa0137110:intron 45.0%
TTTGACAGGCTGCTGTGTTT+GGG - chr1_3:69418817-69418836 Msa0137110:intron 45.0%
!!! TGCAGCTGCTGATTTTTCGT+TGG - chr1_3:69418931-69418950 Msa0137110:intron 45.0%
TGGCAGCCTTCAATATACCT+TGG - chr1_3:69419051-69419070 Msa0137110:intron 45.0%
GCCCAACCAAGGTATATTGA+AGG + chr1_3:69419060-69419079 None:intergenic 45.0%
CAATCGGAGCAACTTTGTCG+AGG - chr1_3:69416388-69416407 Msa0137110:three_prime_UTR 50.0%
GATGTTCACGGATGAAGGGT+CGG - chr1_3:69416528-69416547 Msa0137110:three_prime_UTR 50.0%
CCGTGACCCATTGAGAGATT+AGG - chr1_3:69416574-69416593 Msa0137110:three_prime_UTR 50.0%
TGCAAAGGAAGGTGACACAC+TGG - chr1_3:69417462-69417481 Msa0137110:intron 50.0%
CTGCTATATTCTGTAGGCGG+GGG - chr1_3:69417639-69417658 Msa0137110:CDS 50.0%
!! CAGGAAAGTGCCAATGTCGA+TGG - chr1_3:69417763-69417782 Msa0137110:intron 50.0%
GCTGGAAGCTCCTATTTGTG+TGG - chr1_3:69417796-69417815 Msa0137110:intron 50.0%
CACAGGCCTCATGACAATGA+TGG - chr1_3:69418111-69418130 Msa0137110:intron 50.0%
! GACTGTTTCGTGCTGCTGTT+TGG - chr1_3:69418759-69418778 Msa0137110:intron 50.0%
! CTGTTTGGGTTCATTAGGGG+TGG - chr1_3:69418774-69418793 Msa0137110:intron 50.0%
GTTTGACAGGCTGCTGTGTT+TGG - chr1_3:69418816-69418835 Msa0137110:intron 50.0%
TTGACAGGCTGCTGTGTTTG+GGG - chr1_3:69418818-69418837 Msa0137110:intron 50.0%
! CTGTCATTTGGCTGCTGTTG+TGG - chr1_3:69418895-69418914 Msa0137110:intron 50.0%
TCACAATCTGCTACCCGCAA+AGG + chr1_3:69418971-69418990 None:intergenic 50.0%
TTTGCGGGTAGCAGATTGTG+AGG - chr1_3:69418970-69418989 Msa0137110:intron 50.0%
!! TTGTGAGGCGGAGGTATTCT+AGG - chr1_3:69418985-69419004 Msa0137110:intron 50.0%
!! TGTGAGGCGGAGGTATTCTA+GGG - chr1_3:69418986-69419005 Msa0137110:intron 50.0%
! TTCTAGGGGTGGTAGTACCT+TGG - chr1_3:69419001-69419020 Msa0137110:intron 50.0%
CCACTCCAAGCAATGTACCA+AGG + chr1_3:69419021-69419040 None:intergenic 50.0%
CCTTGGTACATTGCTTGGAG+TGG - chr1_3:69419018-69419037 Msa0137110:intron 50.0%
TGTTCCTTTCCGCGAGTTGT+TGG - chr1_3:69419093-69419112 Msa0137110:intron 50.0%
AACACCAACAACTCGCGGAA+AGG + chr1_3:69419100-69419119 None:intergenic 50.0%
!! TGAAGGGTCGGTGTTACCTG+TGG - chr1_3:69416540-69416559 Msa0137110:three_prime_UTR 55.0%
CACGGTCTCAACTACTCCAC+AGG + chr1_3:69416559-69416578 Msa0137100:CDS 55.0%
! ACAGTGGATAGCAGGGCGTT+TGG - chr1_3:69416975-69416994 Msa0137110:three_prime_UTR 55.0%
! TAGCAGGGCGTTTGGTCTAG+TGG - chr1_3:69416983-69417002 Msa0137110:three_prime_UTR 55.0%
!!! TGGCGTGGCTCTGTTTTAGC+AGG - chr1_3:69418794-69418813 Msa0137110:intron 55.0%
TGACAGGCTGCTGTGTTTGG+GGG - chr1_3:69418819-69418838 Msa0137110:intron 55.0%
!! CAGGTGCTGCTGTTGGTGTT+GGG - chr1_3:69418848-69418867 Msa0137110:intron 55.0%
! TGTTGGGTTGCTGTCGTAGC+AGG - chr1_3:69418864-69418883 Msa0137110:intron 55.0%
!! CAGGTGCTGCTGCTGTCATT+TGG - chr1_3:69418883-69418902 Msa0137110:intron 55.0%
! CTTTCCCCTCCTAGCCTTTG+CGG - chr1_3:69418954-69418973 Msa0137110:intron 55.0%
! TTTCCCCTCCTAGCCTTTGC+GGG - chr1_3:69418955-69418974 Msa0137110:intron 55.0%
ATCTGCTACCCGCAAAGGCT+AGG + chr1_3:69418966-69418985 None:intergenic 55.0%
!! GTGAGGCGGAGGTATTCTAG+GGG - chr1_3:69418987-69419006 Msa0137110:intron 55.0%
TTTGTCGAGGACCACCGTGC+CGG - chr1_3:69416401-69416420 Msa0137110:three_prime_UTR 60.0%
!! CCACTTTTCTGGTGCGGCAG+AGG + chr1_3:69416433-69416452 Msa0137100:intron 60.0%
CCTCTGCCGCACCAGAAAAG+TGG - chr1_3:69416430-69416449 Msa0137110:three_prime_UTR 60.0%
TGGGTTCATTAGGGGTGGCG+TGG - chr1_3:69418779-69418798 Msa0137110:intron 60.0%
! GCTGCTGTGTTTGGGGGTGT+TGG - chr1_3:69418825-69418844 Msa0137110:intron 60.0%
!! CTGTGTTTGGGGGTGTTGGC+AGG - chr1_3:69418829-69418848 Msa0137110:intron 60.0%
!! GTGTTGGCAGGTGCTGCTGT+TGG - chr1_3:69418841-69418860 Msa0137110:intron 60.0%
!! GCAGGTGCTGCTGTTGGTGT+TGG - chr1_3:69418847-69418866 Msa0137110:intron 60.0%
CTACCCGCAAAGGCTAGGAG+GGG + chr1_3:69418961-69418980 None:intergenic 60.0%
GCTACCCGCAAAGGCTAGGA+GGG + chr1_3:69418962-69418981 None:intergenic 60.0%
TGCTACCCGCAAAGGCTAGG+AGG + chr1_3:69418963-69418982 None:intergenic 60.0%
GCGGGTAGCAGATTGTGAGG+CGG - chr1_3:69418973-69418992 Msa0137110:intron 60.0%
GGTAGCAGATTGTGAGGCGG+AGG - chr1_3:69418976-69418995 Msa0137110:intron 60.0%
!! AGGCGGAGGTATTCTAGGGG+TGG - chr1_3:69418990-69419009 Msa0137110:intron 60.0%
AGAGGTGAACTCCGGCACGG+TGG + chr1_3:69416415-69416434 Msa0137100:intron 65.0%
GGCAGAGGTGAACTCCGGCA+CGG + chr1_3:69416418-69416437 Msa0137100:intron 65.0%
GGTGCGGCAGAGGTGAACTC+CGG + chr1_3:69416423-69416442 Msa0137100:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 69416356 69419343 69416356 ID=Msa0137110;Name=Msa0137110
chr1_3 mRNA 69416356 69419343 69416356 ID=Msa0137110-mRNA-1;Parent=Msa0137110;Name=Msa0137110-mRNA-1;_AED=0.23;_eAED=0.26;_QI=0|0.8|0.83|1|0|0|6|740|94
chr1_3 exon 69416356 69416763 69416356 ID=Msa0137110-mRNA-1:exon:24016;Parent=Msa0137110-mRNA-1
chr1_3 exon 69416814 69417066 69416814 ID=Msa0137110-mRNA-1:exon:24015;Parent=Msa0137110-mRNA-1
chr1_3 exon 69417528 69417558 69417528 ID=Msa0137110-mRNA-1:exon:24014;Parent=Msa0137110-mRNA-1
chr1_3 exon 69417584 69417683 69417584 ID=Msa0137110-mRNA-1:exon:24013;Parent=Msa0137110-mRNA-1
chr1_3 exon 69418196 69418282 69418196 ID=Msa0137110-mRNA-1:exon:24012;Parent=Msa0137110-mRNA-1
chr1_3 exon 69419198 69419343 69419198 ID=Msa0137110-mRNA-1:exon:24011;Parent=Msa0137110-mRNA-1
chr1_3 CDS 69419198 69419343 69419198 ID=Msa0137110-mRNA-1:cds;Parent=Msa0137110-mRNA-1
chr1_3 CDS 69418196 69418282 69418196 ID=Msa0137110-mRNA-1:cds;Parent=Msa0137110-mRNA-1
chr1_3 CDS 69417632 69417683 69417632 ID=Msa0137110-mRNA-1:cds;Parent=Msa0137110-mRNA-1
chr1_3 three_prime_UTR 69417584 69417631 69417584 ID=Msa0137110-mRNA-1:three_prime_utr;Parent=Msa0137110-mRNA-1
chr1_3 three_prime_UTR 69417528 69417558 69417528 ID=Msa0137110-mRNA-1:three_prime_utr;Parent=Msa0137110-mRNA-1
chr1_3 three_prime_UTR 69416814 69417066 69416814 ID=Msa0137110-mRNA-1:three_prime_utr;Parent=Msa0137110-mRNA-1
chr1_3 three_prime_UTR 69416356 69416763 69416356 ID=Msa0137110-mRNA-1:three_prime_utr;Parent=Msa0137110-mRNA-1
Gene Sequence

>Msa0137110

ATGTCAGCAACAAAACCCCTAGGTTTATTTTGCAATCGGAGCAACTTTGTCGAGGACCACCGTGCCGGAGTTCACCTCTGCCGCACCAGAAAAGTGGTTTTGTATACGCATTTTCGAATTTTTGAACCAGGACATTATTTAAACATACTCTCTTTGAGGTTCAAATGCAAGCAATTTATTGCCTACATTAGTGCAAAGGAAGGTGACACACTGGTTCCAATTATACACAATAGTGAGGGAGGACTTATGAGATTCGAAGAAAAATTTCAACAAAACATAAGATGA
Protein sequence

>Msa0137110

MSATKPLGLFCNRSNFVEDHRAGVHLCRTRKVVLYTHFRIFEPGHYLNILSLRFKCKQFIAYISAKEGDTLVPIIHNSEGGLMRFEEKFQQNIR*