AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0138190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0138190 MtrunA17_Chr1g0211081 94.061 522 22 4 1 521 6 519 0.0 998
Msa0138190 MtrunA17_Chr8g0365711 28.544 522 259 13 5 518 4 419 2.06e-60 205
Msa0138190 MtrunA17_Chr3g0131691 34.146 205 114 2 311 515 293 476 2.71e-32 129
Msa0138190 MtrunA17_Chr7g0252041 36.275 204 106 4 315 515 285 467 9.14e-31 125
Msa0138190 MtrunA17_Chr2g0298751 34.715 193 107 5 309 498 45 221 3.16e-22 95.9
Msa0138190 MtrunA17_Chr4g0048851 33.529 170 81 3 315 484 46 183 3.28e-20 89.4
Msa0138190 MtrunA17_Chr2g0320291 31.034 174 81 4 301 471 121 258 5.64e-18 85.1
Msa0138190 MtrunA17_Chr4g0028071 31.176 170 70 5 310 467 143 277 2.46e-17 83.6
Msa0138190 MtrunA17_Chr3g0141091 27.962 211 108 4 304 514 161 327 5.89e-16 79.3
Msa0138190 MtrunA17_Chr1g0185621 28.972 214 118 6 310 513 464 653 3.09e-15 78.6
Msa0138190 MtrunA17_Chr8g0346161 30.180 222 113 9 295 506 268 457 6.46e-15 77.0
Msa0138190 MtrunA17_Chr5g0411541 29.609 179 90 3 304 482 144 286 8.77e-15 75.5
Msa0138190 MtrunA17_Chr5g0400661 29.570 186 110 7 309 493 142 307 7.92e-14 72.8
Msa0138190 MtrunA17_Chr7g0260841 42.045 88 50 1 284 371 324 410 1.45e-13 73.2
Msa0138190 MtrunA17_Chr4g0054581 26.012 173 104 4 308 479 190 339 2.83e-13 71.6
Msa0138190 MtrunA17_Chr8g0386921 29.630 189 110 6 296 473 386 562 3.58e-13 72.0
Msa0138190 MtrunA17_Chr7g0225901 27.528 178 102 4 302 479 74 224 4.62e-13 69.3
Msa0138190 MtrunA17_Chr5g0411341 27.679 224 126 8 300 513 472 669 7.29e-13 71.2
Msa0138190 MtrunA17_Chr7g0275821 55.556 54 24 0 320 373 256 309 1.45e-12 69.7
Msa0138190 MtrunA17_Chr8g0366751 29.268 164 94 4 320 480 466 610 5.35e-12 68.6
Msa0138190 MtrunA17_Chr8g0346151 40.260 77 44 1 295 369 256 332 6.50e-12 67.8
Msa0138190 MtrunA17_Chr5g0400671 33.000 100 60 1 288 380 119 218 1.20e-11 66.2
Msa0138190 MtrunA17_Chr4g0048891 37.624 101 57 2 279 373 2 102 1.42e-11 62.4
Msa0138190 MtrunA17_Chr5g0400621 42.222 90 47 3 303 392 145 229 1.76e-11 65.5
Msa0138190 MtrunA17_Chr2g0307131 28.324 173 97 5 307 479 170 315 1.84e-11 65.9
Msa0138190 MtrunA17_Chr4g0049331 49.091 55 28 0 317 371 136 190 2.71e-11 64.7
Msa0138190 MtrunA17_Chr6g0468491 29.630 189 99 8 294 479 116 273 2.95e-11 64.7
Msa0138190 MtrunA17_Chr4g0054571 36.111 72 46 0 300 371 134 205 6.19e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0138190 AT2G16910.1 46.545 550 227 16 1 524 5 513 7.60e-145 429
Msa0138190 AT3G26744.1 35.979 189 98 2 315 500 306 474 8.51e-31 125
Msa0138190 AT3G26744.4 35.979 189 98 2 315 500 306 474 8.51e-31 125
Msa0138190 AT3G26744.2 35.979 189 98 2 315 500 306 474 8.51e-31 125
Msa0138190 AT3G26744.6 36.813 182 92 2 315 493 306 467 2.71e-30 124
Msa0138190 AT1G12860.1 35.323 201 106 3 315 515 266 442 8.01e-30 122
Msa0138190 AT1G12860.2 36.313 179 93 2 315 493 266 423 6.72e-29 119
Msa0138190 AT1G10610.1 35.404 161 88 4 2 152 8 162 9.46e-25 107
Msa0138190 AT1G10610.1 25.962 208 116 4 314 516 262 436 9.09e-16 80.1
Msa0138190 AT5G57150.1 32.212 208 117 5 298 500 32 220 2.31e-20 90.9
Msa0138190 AT5G57150.5 32.338 201 112 5 298 493 32 213 4.84e-20 89.4
Msa0138190 AT5G57150.3 32.338 201 112 5 298 493 33 214 7.86e-20 88.6
Msa0138190 AT5G57150.2 32.338 201 112 5 298 493 33 214 8.03e-20 89.0
Msa0138190 AT5G57150.4 32.338 201 112 5 298 493 33 214 1.03e-19 89.4
Msa0138190 AT2G28160.2 30.061 163 89 4 308 470 123 260 6.46e-17 82.0
Msa0138190 AT4G21330.1 28.155 206 118 4 314 519 30 205 6.57e-17 80.1
Msa0138190 AT2G28160.1 30.061 163 89 4 308 470 123 260 6.92e-17 82.0
Msa0138190 AT1G10610.2 32.836 134 75 3 28 152 5 132 1.17e-16 82.8
Msa0138190 AT1G10610.2 25.962 208 116 4 314 516 232 406 7.80e-16 80.1
Msa0138190 AT5G46760.1 31.677 161 87 4 320 480 419 556 1.80e-16 82.8
Msa0138190 AT5G65640.1 28.959 221 122 5 304 524 166 351 5.22e-16 80.1
Msa0138190 AT1G63650.3 30.814 172 102 4 319 486 408 566 5.85e-16 81.3
Msa0138190 AT1G63650.1 30.814 172 102 4 319 486 408 566 5.85e-16 81.3
Msa0138190 AT1G63650.2 30.814 172 102 4 319 486 408 566 5.85e-16 81.3
Msa0138190 AT1G10610.3 26.486 185 103 3 314 493 165 321 5.98e-16 79.7
Msa0138190 AT4G29930.1 30.928 194 124 5 315 507 53 237 8.25e-16 77.8
Msa0138190 AT2G46510.1 44.086 93 47 1 301 388 375 467 8.87e-16 80.9
Msa0138190 AT4G17880.1 29.570 186 99 5 300 480 395 553 5.26e-15 78.2
Msa0138190 AT5G65640.2 57.746 71 30 0 304 374 166 236 5.90e-15 75.9
Msa0138190 AT5G10570.1 29.717 212 103 6 306 512 138 308 7.42e-15 76.3
Msa0138190 AT4G29930.3 30.508 177 115 4 315 490 53 222 8.31e-15 75.1
Msa0138190 AT4G29930.7 30.508 177 115 4 315 490 53 222 8.31e-15 75.1
Msa0138190 AT4G29930.4 55.000 60 27 0 315 374 53 112 4.82e-14 71.2
Msa0138190 AT4G29930.2 55.000 60 27 0 315 374 53 112 5.62e-14 72.0
Msa0138190 AT4G29930.5 55.000 60 27 0 315 374 53 112 5.95e-14 72.0
Msa0138190 AT4G29930.6 55.000 60 27 0 315 374 53 112 6.21e-14 71.6
Msa0138190 AT5G46830.1 27.805 205 114 6 310 514 337 507 9.28e-13 70.9
Msa0138190 AT4G37850.2 27.059 170 97 4 311 479 147 290 1.23e-12 69.7
Msa0138190 AT4G37850.1 27.059 170 97 4 311 479 147 290 1.23e-12 69.7
Msa0138190 AT5G41315.1 28.977 176 105 6 319 486 444 607 2.02e-12 70.1
Msa0138190 AT5G41315.3 28.877 187 110 7 308 486 441 612 2.04e-12 70.1
Msa0138190 AT1G01260.1 42.045 88 49 2 287 373 404 490 2.21e-12 70.1
Msa0138190 AT1G01260.2 42.045 88 49 2 287 373 404 490 2.21e-12 70.1
Msa0138190 AT1G01260.3 42.045 88 49 2 287 373 404 490 2.21e-12 70.1
Msa0138190 AT5G41315.2 28.977 176 105 6 319 486 452 615 2.25e-12 70.1
Msa0138190 AT4G09820.1 26.786 168 74 3 317 482 364 484 3.78e-12 68.9
Msa0138190 AT4G16430.1 43.421 76 38 1 301 371 300 375 5.89e-12 68.2
Msa0138190 AT1G32640.1 28.205 195 109 4 320 514 456 619 9.83e-12 68.2
Msa0138190 AT2G22750.4 26.471 170 103 3 310 479 148 295 5.34e-11 64.7
Msa0138190 AT2G22750.5 26.471 170 103 3 310 479 144 291 5.72e-11 64.3
Msa0138190 AT2G22750.3 26.471 170 103 3 310 479 148 295 5.99e-11 64.3
Msa0138190 AT2G22750.1 26.471 170 103 3 310 479 120 267 7.37e-11 63.9
Msa0138190 AT4G00870.1 40.323 62 37 0 320 381 253 314 7.77e-11 64.7
Msa0138190 AT2G22750.2 26.471 170 103 3 310 479 120 267 8.22e-11 63.5

Find 108 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGATAGAGCTTCTATTCT+TGG 0.152880 1_3:+70976290 Msa0138190:CDS
GGAACATTGTTATTATTATT+TGG 0.155242 1_3:-70976437 None:intergenic
CTATCAAGCTTTGAGATTCT+TGG 0.205780 1_3:-70976275 None:intergenic
CTCTTGTTGGTTTGAATGGC+TGG 0.239275 1_3:+70974404 Msa0138190:CDS
TGAAATCCATGTTCTGCTTT+TGG 0.239465 1_3:-70976458 None:intergenic
ATAGAATTATGAACAGAAAA+AGG 0.296948 1_3:+70976983 Msa0138190:intron
CAGCAACAAGGTTCTTTGAT+TGG 0.308174 1_3:-70976196 None:intergenic
GTGGAAGTGGCTCAGATAGA+TGG 0.338551 1_3:+70976701 Msa0138190:CDS
GTTATATAACCTTCGTTCAT+TGG 0.349149 1_3:+70976250 Msa0138190:CDS
AGATCACAAGAGTTTGTTCT+TGG 0.351468 1_3:-70974692 None:intergenic
GGTGTTCTTCTCCACCATTT+TGG 0.356060 1_3:-70974628 None:intergenic
GAACTTACATGTAAAGTGTT+TGG 0.359394 1_3:-70974918 None:intergenic
GAAGGAAGAAACTGAATGAT+AGG 0.368436 1_3:+70976228 Msa0138190:CDS
CAAATCTTCATGCTGGAACT+TGG 0.371640 1_3:+70977064 Msa0138190:CDS
AGACCTCTTGTTGGTTTGAA+TGG 0.376737 1_3:+70974400 Msa0138190:CDS
GTGTTTGGATCCAAGCTATT+GGG 0.381111 1_3:-70974903 None:intergenic
TAGGTATGGAAGTGGAAAGT+TGG 0.382059 1_3:-70974721 None:intergenic
ATTGGGATAGTTCAACCAAT+TGG 0.386734 1_3:-70974886 None:intergenic
GATTCCAGTGCCTGGTGGAC+TGG 0.406904 1_3:+70975071 Msa0138190:CDS
CAGAACATGGATTTCATGTT+GGG 0.411727 1_3:+70976465 Msa0138190:CDS
TATATATACCCTGAATCTAT+AGG 0.413784 1_3:-70974740 None:intergenic
TGAGCTCTTTGTAACTAAAC+AGG 0.419409 1_3:+70975095 Msa0138190:CDS
GGTTGGGATGTTGTTGTGGT+GGG 0.420257 1_3:+70974594 Msa0138190:CDS
AAGAGGATGATGGAAAATAT+AGG 0.425274 1_3:+70976153 Msa0138190:CDS
AGTGTTTGGATCCAAGCTAT+TGG 0.426318 1_3:-70974904 None:intergenic
ACTTCCATACCTATAGATTC+AGG 0.426419 1_3:+70974731 Msa0138190:CDS
TTTCCCTGTCTCTTCATGTA+GGG 0.439137 1_3:+70974652 Msa0138190:CDS
TTGGGATAGTTCAACCAATT+GGG 0.440118 1_3:-70974885 None:intergenic
TATTAACTAACCAACCCAAT+TGG 0.442270 1_3:+70974871 Msa0138190:CDS
TATAGGAGAAGAAGTGGAAA+AGG 0.445604 1_3:+70976170 Msa0138190:CDS
AGGTATGGAAGTGGAAAGTT+GGG 0.445933 1_3:-70974720 None:intergenic
GAAGGGATGAACCACTCAAT+TGG 0.446434 1_3:+70975732 Msa0138190:CDS
GAGAGATTAAGACCTCTTGT+TGG 0.450531 1_3:+70974391 Msa0138190:CDS
ATGGAGGCTTTGAACACTTT+AGG 0.460171 1_3:+70976782 Msa0138190:CDS
ATATATATAGGTATCTTGAA+TGG 0.460510 1_3:+70974573 Msa0138190:intron
AGTAACTGTGTATATGTTGA+TGG 0.466614 1_3:+70976401 Msa0138190:CDS
ATCATGCACCTCTTCTGTGT+TGG 0.467913 1_3:-70976006 None:intergenic
TGGTTGGGATGTTGTTGTGG+TGG 0.468844 1_3:+70974593 Msa0138190:CDS
GCAGAACATGGATTTCATGT+TGG 0.469960 1_3:+70976464 Msa0138190:CDS
GGAAAATATAGGAGAAGAAG+TGG 0.472548 1_3:+70976164 Msa0138190:CDS
GATCACAAGAGTTTGTTCTT+GGG 0.473126 1_3:-70974691 None:intergenic
TCTTGTTGGTTTGAATGGCT+GGG 0.473478 1_3:+70974405 Msa0138190:CDS
AAGAAGCAAGAACATGTTGT+TGG 0.475916 1_3:+70976089 Msa0138190:CDS
GCTGGGATTACTGTGTCTAC+TGG 0.476054 1_3:+70974422 Msa0138190:CDS
ATATAGGTATCTTGAATGGT+TGG 0.479738 1_3:+70974577 Msa0138190:intron
GGCTTTGAACACTTTAGGCA+TGG 0.486692 1_3:+70976787 Msa0138190:CDS
ATGAATATTGCAGGCACAAG+TGG 0.489997 1_3:+70976682 Msa0138190:intron
TATAGGTATCTTGAATGGTT+GGG 0.493698 1_3:+70974578 Msa0138190:intron
GATTCAATGTGTCTGATGAA+TGG 0.495437 1_3:-70975833 None:intergenic
TAATAATCAATATATGTTTG+TGG 0.498103 1_3:+70976049 Msa0138190:CDS
GTTGAACTATCCCAATAGCT+TGG 0.501147 1_3:+70974893 Msa0138190:CDS
CAGAATGAAGAAGAGGATGA+TGG 0.501916 1_3:+70976143 Msa0138190:CDS
ATTGAACAGGATTCATGCAC+AGG 0.504490 1_3:+70974845 Msa0138190:intron
TTCTCTTCCTCTCAGCAACA+AGG 0.506548 1_3:-70976208 None:intergenic
GAATGGTTGGGATGTTGTTG+TGG 0.510919 1_3:+70974590 Msa0138190:CDS
GTATCCCTACATGAAGAGAC+AGG 0.511754 1_3:-70974656 None:intergenic
ATACCCTGAATCTATAGGTA+TGG 0.513797 1_3:-70974735 None:intergenic
GGGAGTGAAAGCAACCAAAA+TGG 0.520448 1_3:+70974614 Msa0138190:CDS
TGGAAGTGGCTCAGATAGAT+GGG 0.522256 1_3:+70976702 Msa0138190:CDS
TGAGACAAGACCCTTGTGAC+TGG 0.522381 1_3:-70976831 None:intergenic
CATGGATTTCATGTTGGGAA+TGG 0.525371 1_3:+70976470 Msa0138190:CDS
ATTGCAGGCACAAGTGGAAG+TGG 0.525672 1_3:+70976688 Msa0138190:intron
TGAATCTATAGGTATGGAAG+TGG 0.528440 1_3:-70974729 None:intergenic
CACGGAACAAATCTTCATGC+TGG 0.531543 1_3:+70977057 Msa0138190:CDS
CTTCCATACCTATAGATTCA+GGG 0.537250 1_3:+70974732 Msa0138190:CDS
ATGCAATCGAATCCAATTGT+GGG 0.539218 1_3:+70975774 Msa0138190:CDS
CTATGTTTCAAAACAAAAGC+AGG 0.545019 1_3:+70976493 Msa0138190:CDS
GGAAATTAAGTGAAGATCAA+AGG 0.546606 1_3:+70974443 Msa0138190:CDS
CATGCAATCGAATCCAATTG+TGG 0.546804 1_3:+70975773 Msa0138190:CDS
GATAGTTCAACCAATTGGGT+TGG 0.554141 1_3:-70974881 None:intergenic
TACATGAAGAGACAGGGAAA+AGG 0.554895 1_3:-70974649 None:intergenic
TTGAATTGAAACCAATTGAG+TGG 0.557955 1_3:-70975743 None:intergenic
CAGGCACTGGAATCAAAACA+TGG 0.559119 1_3:-70975062 None:intergenic
CAGTGATCAGAATGAAGAAG+AGG 0.559858 1_3:+70976136 Msa0138190:CDS
TTGTTGCTGAGAGGAAGAGA+AGG 0.562094 1_3:+70976210 Msa0138190:CDS
AAGAGCTCAACCAGTCCACC+AGG 0.565516 1_3:-70975081 None:intergenic
ACCTTGGTATCCTTCAGAGA+AGG 0.568708 1_3:-70975943 None:intergenic
ATCAATTTCACAAAACCACC+TGG 0.575508 1_3:-70976761 None:intergenic
GATCATGAAGAAAAGAACCT+TGG 0.576590 1_3:-70975959 None:intergenic
TCAACCAGTCCACCAGGCAC+TGG 0.579883 1_3:-70975075 None:intergenic
AAACAAAAGCAGGAAGATGT+TGG 0.583119 1_3:+70976503 Msa0138190:CDS
ATCTCAATGCATTGTCATGG+TGG 0.583356 1_3:+70975704 Msa0138190:CDS
TGACAAGCAGACACAGCAGA+TGG 0.586592 1_3:+70976535 Msa0138190:CDS
AAAGGAGAGTGAAATAGTTG+AGG 0.586665 1_3:+70977001 Msa0138190:CDS
CACATCTCAATGCATTGTCA+TGG 0.588350 1_3:+70975701 Msa0138190:CDS
AACAACGAAACCTTCTCTGA+AGG 0.589214 1_3:+70975933 Msa0138190:CDS
TTCAATACAGATATGTCATG+TGG 0.598259 1_3:-70975858 None:intergenic
AGAGCTTCAAGATGAACTTG+AGG 0.601650 1_3:+70976355 Msa0138190:CDS
AATGTTCCAAAAGCAGAACA+TGG 0.606756 1_3:+70976452 Msa0138190:CDS
TCTCAAATCCAACACAGAAG+AGG 0.606938 1_3:+70975998 Msa0138190:CDS
ATTGTCATGGTGGATCAAGA+AGG 0.607678 1_3:+70975714 Msa0138190:CDS
ACCTTCTCTGAAGGATACCA+AGG 0.618882 1_3:+70975942 Msa0138190:CDS
CAAAGAACCTTGTTGCTGAG+AGG 0.619036 1_3:+70976201 Msa0138190:CDS
CTTGAGGAAAATTCAGACAC+AGG 0.625559 1_3:+70976371 Msa0138190:CDS
TGCCACAGTAACCAGTCACA+AGG 0.625627 1_3:+70976820 Msa0138190:CDS
AGATGCTATTGAATTTGTGA+AGG 0.628469 1_3:+70976313 Msa0138190:CDS
CAGCCATTCAAACCAACAAG+AGG 0.632565 1_3:-70974403 None:intergenic
ATTGTGGGTGATGAAAATGA+TGG 0.641485 1_3:+70975789 Msa0138190:CDS
AGTGAAAGCAACCAAAATGG+TGG 0.646392 1_3:+70974617 Msa0138190:CDS
ATTCACTACTAGAGCTGACA+CGG 0.647595 1_3:+70977039 Msa0138190:CDS
TTCTTGGAACCAATGAACGA+AGG 0.654816 1_3:-70976259 None:intergenic
ACCCTTGTGACTGGTTACTG+TGG 0.657104 1_3:-70976822 None:intergenic
TTGTCATGGTGGATCAAGAA+GGG 0.669108 1_3:+70975715 Msa0138190:CDS
TGGGAATGAGTACTTTGTGA+AGG 0.678653 1_3:+70976721 Msa0138190:CDS
CAAGCAGACACAGCAGATGG+AGG 0.682677 1_3:+70976538 Msa0138190:CDS
TATCCCTACATGAAGAGACA+GGG 0.687876 1_3:-70974655 None:intergenic
GCCACAGTAACCAGTCACAA+GGG 0.696830 1_3:+70976821 Msa0138190:CDS
AGGCACTGGAATCAAAACAT+GGG 0.706180 1_3:-70975061 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAATAGAACAAAAAATA+TGG - chr1_3:70974503-70974522 None:intergenic 10.0%
!!! TAAATTTTAAATTAAGCTAA+AGG - chr1_3:70975192-70975211 None:intergenic 10.0%
!! AGTTATTAGTATATTTATTT+AGG - chr1_3:70975627-70975646 None:intergenic 10.0%
!! AATTTATATACTAATAACAA+AGG + chr1_3:70976586-70976605 Msa0138190:intron 10.0%
!!! ATGATATTAAATTTTTTTCT+TGG + chr1_3:70976951-70976970 Msa0138190:intron 10.0%
!!! TGATATTAAATTTTTTTCTT+GGG + chr1_3:70976952-70976971 Msa0138190:intron 10.0%
!!! TTGTTGGTTTTTATATATAT+AGG + chr1_3:70974561-70974580 Msa0138190:intron 15.0%
!! TTATATATAGATAGAGTAAA+GGG - chr1_3:70974810-70974829 None:intergenic 15.0%
!! ATTATATATAGATAGAGTAA+AGG - chr1_3:70974811-70974830 None:intergenic 15.0%
!! AAAATAGTACTCAATATATA+TGG - chr1_3:70975020-70975039 None:intergenic 15.0%
!!! ACTTTTTTGAATTATTATCA+AGG + chr1_3:70975465-70975484 Msa0138190:intron 15.0%
!! CATAATAAAAATATGAAGTT+CGG + chr1_3:70975498-70975517 Msa0138190:intron 15.0%
!! TAATAATCAATATATGTTTG+TGG + chr1_3:70976049-70976068 Msa0138190:CDS 15.0%
!!! TATAAATTTTAGTATAAGCT+AGG - chr1_3:70976574-70976593 None:intergenic 15.0%
!!! TAATTGTGATGTTAATTTGT+TGG + chr1_3:70974545-70974564 Msa0138190:intron 20.0%
!! ATATATATAGGTATCTTGAA+TGG + chr1_3:70974573-70974592 Msa0138190:intron 20.0%
!! AGATGAATTAGTTGAAAAAT+AGG - chr1_3:70974959-70974978 None:intergenic 20.0%
!!! TTTTTCAACTAATTCATCTT+TGG + chr1_3:70974960-70974979 Msa0138190:intron 20.0%
!! TATAAGTAGGTAGATATAAA+TGG + chr1_3:70975155-70975174 Msa0138190:intron 20.0%
!! TCATTGAATGATATACTATA+AGG + chr1_3:70975307-70975326 Msa0138190:intron 20.0%
!!! TAAGATCATGTTAATTTTGA+TGG + chr1_3:70975406-70975425 Msa0138190:intron 20.0%
!!! TGAATTATTATCAAGGTTTT+TGG + chr1_3:70975472-70975491 Msa0138190:intron 20.0%
!!! GGAACATTGTTATTATTATT+TGG - chr1_3:70976440-70976459 None:intergenic 20.0%
!! AAGTTAAGAAAATGATTCTA+AGG - chr1_3:70976882-70976901 None:intergenic 20.0%
!! AGACAAATTCAAATCAAAAT+AGG + chr1_3:70976918-70976937 Msa0138190:intron 20.0%
!! ATAGAATTATGAACAGAAAA+AGG + chr1_3:70976983-70977002 Msa0138190:intron 20.0%
! TATATATACCCTGAATCTAT+AGG - chr1_3:70974743-70974762 None:intergenic 25.0%
! TATATGGAATTAGCTGAAAA+AGG - chr1_3:70975004-70975023 None:intergenic 25.0%
!!! ATATTGAGTACTATTTTTGC+AGG + chr1_3:70975023-70975042 Msa0138190:intron 25.0%
!! TTTTTGCAGGAAACAATTAA+TGG + chr1_3:70975036-70975055 Msa0138190:intron 25.0%
!! TTTTGCAGGAAACAATTAAT+GGG + chr1_3:70975037-70975056 Msa0138190:intron 25.0%
! TTAGAAACACACATATAAGT+AGG + chr1_3:70975142-70975161 Msa0138190:intron 25.0%
! GCTTAATTTACATATTGAAC+TGG + chr1_3:70975247-70975266 Msa0138190:intron 25.0%
! TTCTCTGTGTATAATATAAC+AGG + chr1_3:70975647-70975666 Msa0138190:intron 25.0%
!!! CTCAAATGTTTTTAAAGTTG+AGG + chr1_3:70976850-70976869 Msa0138190:CDS 25.0%
GGAAATTAAGTGAAGATCAA+AGG + chr1_3:70974443-70974462 Msa0138190:CDS 30.0%
ATATAGGTATCTTGAATGGT+TGG + chr1_3:70974577-70974596 Msa0138190:intron 30.0%
TATAGGTATCTTGAATGGTT+GGG + chr1_3:70974578-70974597 Msa0138190:intron 30.0%
ATATAGATAGAGTAAAGGGA+GGG - chr1_3:70974806-70974825 None:intergenic 30.0%
TATATAGATAGAGTAAAGGG+AGG - chr1_3:70974807-70974826 None:intergenic 30.0%
CTGTTCAATGAAGAGATTAA+AGG - chr1_3:70974834-70974853 None:intergenic 30.0%
CTTTAATCTCTTCATTGAAC+AGG + chr1_3:70974832-70974851 Msa0138190:intron 30.0%
TATTAACTAACCAACCCAAT+TGG + chr1_3:70974871-70974890 Msa0138190:CDS 30.0%
! GAACTTACATGTAAAGTGTT+TGG - chr1_3:70974921-70974940 None:intergenic 30.0%
CACTTTACATGTAAGTTCAT+TGG + chr1_3:70974923-70974942 Msa0138190:intron 30.0%
GGTCTAATTATGATGTTGAA+GGG - chr1_3:70975342-70975361 None:intergenic 30.0%
TGATCTTGATGTTGTTAACT+GGG - chr1_3:70975365-70975384 None:intergenic 30.0%
!! TATAAAAGCGTCAAAGATGT+TGG - chr1_3:70975525-70975544 None:intergenic 30.0%
CTGTGTATAATATAACAGGT+TGG + chr1_3:70975651-70975670 Msa0138190:intron 30.0%
TTGAATTGAAACCAATTGAG+TGG - chr1_3:70975746-70975765 None:intergenic 30.0%
TTCAATACAGATATGTCATG+TGG - chr1_3:70975861-70975880 None:intergenic 30.0%
!!! GCTTCTTTTTTGTGTCTAAT+GGG - chr1_3:70976076-70976095 None:intergenic 30.0%
!!! TGCTTCTTTTTTGTGTCTAA+TGG - chr1_3:70976077-70976096 None:intergenic 30.0%
!! AAGAGGATGATGGAAAATAT+AGG + chr1_3:70976153-70976172 Msa0138190:CDS 30.0%
GTTATATAACCTTCGTTCAT+TGG + chr1_3:70976250-70976269 Msa0138190:CDS 30.0%
AGATGCTATTGAATTTGTGA+AGG + chr1_3:70976313-70976332 Msa0138190:CDS 30.0%
AGTAACTGTGTATATGTTGA+TGG + chr1_3:70976401-70976420 Msa0138190:CDS 30.0%
CTATGTTTCAAAACAAAAGC+AGG + chr1_3:70976493-70976512 Msa0138190:CDS 30.0%
CACATGAATATGAATATTGC+AGG + chr1_3:70976673-70976692 Msa0138190:intron 30.0%
! GATCACAAGAGTTTGTTCTT+GGG - chr1_3:70974694-70974713 None:intergenic 35.0%
! AGATCACAAGAGTTTGTTCT+TGG - chr1_3:70974695-70974714 None:intergenic 35.0%
TGAATCTATAGGTATGGAAG+TGG - chr1_3:70974732-70974751 None:intergenic 35.0%
ACTTCCATACCTATAGATTC+AGG + chr1_3:70974731-70974750 Msa0138190:CDS 35.0%
CTTCCATACCTATAGATTCA+GGG + chr1_3:70974732-70974751 Msa0138190:CDS 35.0%
ATACCCTGAATCTATAGGTA+TGG - chr1_3:70974738-70974757 None:intergenic 35.0%
TTGGGATAGTTCAACCAATT+GGG - chr1_3:70974888-70974907 None:intergenic 35.0%
ATTGGGATAGTTCAACCAAT+TGG - chr1_3:70974889-70974908 None:intergenic 35.0%
TGAGCTCTTTGTAACTAAAC+AGG + chr1_3:70975095-70975114 Msa0138190:CDS 35.0%
GGGTCTAATTATGATGTTGA+AGG - chr1_3:70975343-70975362 None:intergenic 35.0%
ATCTTGATGTTGTTAACTGG+GGG - chr1_3:70975363-70975382 None:intergenic 35.0%
GATCTTGATGTTGTTAACTG+GGG - chr1_3:70975364-70975383 None:intergenic 35.0%
GTGATCTTGATGTTGTTAAC+TGG - chr1_3:70975366-70975385 None:intergenic 35.0%
TTTAGGCAAAACTGCATCAA+AGG - chr1_3:70975610-70975629 None:intergenic 35.0%
ATGCAATCGAATCCAATTGT+GGG + chr1_3:70975774-70975793 Msa0138190:CDS 35.0%
! TCATTTTCATCACCCACAAT+TGG - chr1_3:70975789-70975808 None:intergenic 35.0%
ATTGTGGGTGATGAAAATGA+TGG + chr1_3:70975789-70975808 Msa0138190:CDS 35.0%
GATTCAATGTGTCTGATGAA+TGG - chr1_3:70975836-70975855 None:intergenic 35.0%
GATCATGAAGAAAAGAACCT+TGG - chr1_3:70975962-70975981 None:intergenic 35.0%
AAGAAGCAAGAACATGTTGT+TGG + chr1_3:70976089-70976108 Msa0138190:CDS 35.0%
GGAAAATATAGGAGAAGAAG+TGG + chr1_3:70976164-70976183 Msa0138190:CDS 35.0%
TATAGGAGAAGAAGTGGAAA+AGG + chr1_3:70976170-70976189 Msa0138190:CDS 35.0%
GAAGGAAGAAACTGAATGAT+AGG + chr1_3:70976228-70976247 Msa0138190:CDS 35.0%
!! CTATCAAGCTTTGAGATTCT+TGG - chr1_3:70976278-70976297 None:intergenic 35.0%
CTTGATAGAGCTTCTATTCT+TGG + chr1_3:70976290-70976309 Msa0138190:CDS 35.0%
AATGTTCCAAAAGCAGAACA+TGG + chr1_3:70976452-70976471 Msa0138190:CDS 35.0%
! TGAAATCCATGTTCTGCTTT+TGG - chr1_3:70976461-70976480 None:intergenic 35.0%
CAGAACATGGATTTCATGTT+GGG + chr1_3:70976465-70976484 Msa0138190:CDS 35.0%
AAACAAAAGCAGGAAGATGT+TGG + chr1_3:70976503-70976522 Msa0138190:CDS 35.0%
ATCAATTTCACAAAACCACC+TGG - chr1_3:70976764-70976783 None:intergenic 35.0%
!!! AGGTGGTTTTGTGAAATTGA+TGG + chr1_3:70976763-70976782 Msa0138190:CDS 35.0%
!!! TGGTTTTGTGAAATTGATGG+AGG + chr1_3:70976766-70976785 Msa0138190:CDS 35.0%
AAAGGAGAGTGAAATAGTTG+AGG + chr1_3:70977001-70977020 Msa0138190:CDS 35.0%
GAGAGATTAAGACCTCTTGT+TGG + chr1_3:70974391-70974410 Msa0138190:CDS 40.0%
!! AGACCTCTTGTTGGTTTGAA+TGG + chr1_3:70974400-70974419 Msa0138190:CDS 40.0%
!! TCTTGTTGGTTTGAATGGCT+GGG + chr1_3:70974405-70974424 Msa0138190:CDS 40.0%
AGTGAAAGCAACCAAAATGG+TGG + chr1_3:70974617-70974636 Msa0138190:CDS 40.0%
TACATGAAGAGACAGGGAAA+AGG - chr1_3:70974652-70974671 None:intergenic 40.0%
! TTTTCCCTGTCTCTTCATGT+AGG + chr1_3:70974651-70974670 Msa0138190:CDS 40.0%
TTTCCCTGTCTCTTCATGTA+GGG + chr1_3:70974652-70974671 Msa0138190:CDS 40.0%
TATCCCTACATGAAGAGACA+GGG - chr1_3:70974658-70974677 None:intergenic 40.0%
AGGTATGGAAGTGGAAAGTT+GGG - chr1_3:70974723-70974742 None:intergenic 40.0%
TAGGTATGGAAGTGGAAAGT+TGG - chr1_3:70974724-70974743 None:intergenic 40.0%
ATTGAACAGGATTCATGCAC+AGG + chr1_3:70974845-70974864 Msa0138190:intron 40.0%
GATAGTTCAACCAATTGGGT+TGG - chr1_3:70974884-70974903 None:intergenic 40.0%
GTTGAACTATCCCAATAGCT+TGG + chr1_3:70974893-70974912 Msa0138190:CDS 40.0%
GTGTTTGGATCCAAGCTATT+GGG - chr1_3:70974906-70974925 None:intergenic 40.0%
AGTGTTTGGATCCAAGCTAT+TGG - chr1_3:70974907-70974926 None:intergenic 40.0%
!! AGGCACTGGAATCAAAACAT+GGG - chr1_3:70975064-70975083 None:intergenic 40.0%
CACATCTCAATGCATTGTCA+TGG + chr1_3:70975701-70975720 Msa0138190:CDS 40.0%
ATCTCAATGCATTGTCATGG+TGG + chr1_3:70975704-70975723 Msa0138190:CDS 40.0%
!! ATTGTCATGGTGGATCAAGA+AGG + chr1_3:70975714-70975733 Msa0138190:CDS 40.0%
!! TTGTCATGGTGGATCAAGAA+GGG + chr1_3:70975715-70975734 Msa0138190:CDS 40.0%
CATGCAATCGAATCCAATTG+TGG + chr1_3:70975773-70975792 Msa0138190:CDS 40.0%
AACAACGAAACCTTCTCTGA+AGG + chr1_3:70975933-70975952 Msa0138190:CDS 40.0%
TCTCAAATCCAACACAGAAG+AGG + chr1_3:70975998-70976017 Msa0138190:CDS 40.0%
CAGTGATCAGAATGAAGAAG+AGG + chr1_3:70976136-70976155 Msa0138190:CDS 40.0%
CAGAATGAAGAAGAGGATGA+TGG + chr1_3:70976143-70976162 Msa0138190:CDS 40.0%
CAGCAACAAGGTTCTTTGAT+TGG - chr1_3:70976199-70976218 None:intergenic 40.0%
TTCTTGGAACCAATGAACGA+AGG - chr1_3:70976262-70976281 None:intergenic 40.0%
AGAGCTTCAAGATGAACTTG+AGG + chr1_3:70976355-70976374 Msa0138190:CDS 40.0%
CTTGAGGAAAATTCAGACAC+AGG + chr1_3:70976371-70976390 Msa0138190:CDS 40.0%
GCAGAACATGGATTTCATGT+TGG + chr1_3:70976464-70976483 Msa0138190:CDS 40.0%
CATGGATTTCATGTTGGGAA+TGG + chr1_3:70976470-70976489 Msa0138190:CDS 40.0%
ATGAATATTGCAGGCACAAG+TGG + chr1_3:70976682-70976701 Msa0138190:intron 40.0%
TGGGAATGAGTACTTTGTGA+AGG + chr1_3:70976721-70976740 Msa0138190:CDS 40.0%
!! ATGGAGGCTTTGAACACTTT+AGG + chr1_3:70976782-70976801 Msa0138190:CDS 40.0%
ATTCACTACTAGAGCTGACA+CGG + chr1_3:70977039-70977058 Msa0138190:CDS 40.0%
CAAATCTTCATGCTGGAACT+TGG + chr1_3:70977064-70977083 Msa0138190:CDS 40.0%
CAGCCATTCAAACCAACAAG+AGG - chr1_3:70974406-70974425 None:intergenic 45.0%
!! CTCTTGTTGGTTTGAATGGC+TGG + chr1_3:70974404-70974423 Msa0138190:CDS 45.0%
GAATGGTTGGGATGTTGTTG+TGG + chr1_3:70974590-70974609 Msa0138190:CDS 45.0%
GGGAGTGAAAGCAACCAAAA+TGG + chr1_3:70974614-70974633 Msa0138190:CDS 45.0%
! GGTGTTCTTCTCCACCATTT+TGG - chr1_3:70974631-70974650 None:intergenic 45.0%
GTATCCCTACATGAAGAGAC+AGG - chr1_3:70974659-70974678 None:intergenic 45.0%
!! CAGGCACTGGAATCAAAACA+TGG - chr1_3:70975065-70975084 None:intergenic 45.0%
!!! CATGTTTTGATTCCAGTGCC+TGG + chr1_3:70975063-70975082 Msa0138190:CDS 45.0%
GAAGGGATGAACCACTCAAT+TGG + chr1_3:70975732-70975751 Msa0138190:CDS 45.0%
! ACCTTCTCTGAAGGATACCA+AGG + chr1_3:70975942-70975961 Msa0138190:CDS 45.0%
ACCTTGGTATCCTTCAGAGA+AGG - chr1_3:70975946-70975965 None:intergenic 45.0%
ATCATGCACCTCTTCTGTGT+TGG - chr1_3:70976009-70976028 None:intergenic 45.0%
CAAAGAACCTTGTTGCTGAG+AGG + chr1_3:70976201-70976220 Msa0138190:CDS 45.0%
TTCTCTTCCTCTCAGCAACA+AGG - chr1_3:70976211-70976230 None:intergenic 45.0%
TTGTTGCTGAGAGGAAGAGA+AGG + chr1_3:70976210-70976229 Msa0138190:CDS 45.0%
TGGAAGTGGCTCAGATAGAT+GGG + chr1_3:70976702-70976721 Msa0138190:CDS 45.0%
!!! TGAAGGTGTTTTGTGAGCAC+AGG + chr1_3:70976738-70976757 Msa0138190:CDS 45.0%
!! GGCTTTGAACACTTTAGGCA+TGG + chr1_3:70976787-70976806 Msa0138190:CDS 45.0%
CACGGAACAAATCTTCATGC+TGG + chr1_3:70977057-70977076 Msa0138190:CDS 45.0%
GCTGGGATTACTGTGTCTAC+TGG + chr1_3:70974422-70974441 Msa0138190:CDS 50.0%
TGGTTGGGATGTTGTTGTGG+TGG + chr1_3:70974593-70974612 Msa0138190:CDS 50.0%
GGTTGGGATGTTGTTGTGGT+GGG + chr1_3:70974594-70974613 Msa0138190:CDS 50.0%
!!! GTTTTGATTCCAGTGCCTGG+TGG + chr1_3:70975066-70975085 Msa0138190:CDS 50.0%
TGACAAGCAGACACAGCAGA+TGG + chr1_3:70976535-70976554 Msa0138190:CDS 50.0%
ATTGCAGGCACAAGTGGAAG+TGG + chr1_3:70976688-70976707 Msa0138190:intron 50.0%
GTGGAAGTGGCTCAGATAGA+TGG + chr1_3:70976701-70976720 Msa0138190:CDS 50.0%
TGCCACAGTAACCAGTCACA+AGG + chr1_3:70976820-70976839 Msa0138190:CDS 50.0%
GCCACAGTAACCAGTCACAA+GGG + chr1_3:70976821-70976840 Msa0138190:CDS 50.0%
! ACCCTTGTGACTGGTTACTG+TGG - chr1_3:70976825-70976844 None:intergenic 50.0%
TGAGACAAGACCCTTGTGAC+TGG - chr1_3:70976834-70976853 None:intergenic 50.0%
AAGAGCTCAACCAGTCCACC+AGG - chr1_3:70975084-70975103 None:intergenic 55.0%
CAAGCAGACACAGCAGATGG+AGG + chr1_3:70976538-70976557 Msa0138190:CDS 55.0%
! GTGTTTTGTGAGCACAGGCC+AGG + chr1_3:70976743-70976762 Msa0138190:CDS 55.0%
! TTTTGTGAGCACAGGCCAGG+TGG + chr1_3:70976746-70976765 Msa0138190:CDS 55.0%
! GATTCCAGTGCCTGGTGGAC+TGG + chr1_3:70975071-70975090 Msa0138190:CDS 60.0%
TCAACCAGTCCACCAGGCAC+TGG - chr1_3:70975078-70975097 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 70974376 70977094 70974376 ID=Msa0138190;Name=Msa0138190
chr1_3 mRNA 70974376 70977094 70974376 ID=Msa0138190-mRNA-1;Parent=Msa0138190;Name=Msa0138190-mRNA-1;_AED=0.08;_eAED=0.09;_QI=0|0|0|1|1|1|7|0|528
chr1_3 exon 70974376 70974464 70974376 ID=Msa0138190-mRNA-1:exon:24636;Parent=Msa0138190-mRNA-1
chr1_3 exon 70974583 70974753 70974583 ID=Msa0138190-mRNA-1:exon:24637;Parent=Msa0138190-mRNA-1
chr1_3 exon 70974854 70974932 70974854 ID=Msa0138190-mRNA-1:exon:24638;Parent=Msa0138190-mRNA-1
chr1_3 exon 70975045 70975116 70975045 ID=Msa0138190-mRNA-1:exon:24639;Parent=Msa0138190-mRNA-1
chr1_3 exon 70975669 70976559 70975669 ID=Msa0138190-mRNA-1:exon:24640;Parent=Msa0138190-mRNA-1
chr1_3 exon 70976695 70976871 70976695 ID=Msa0138190-mRNA-1:exon:24641;Parent=Msa0138190-mRNA-1
chr1_3 exon 70976987 70977094 70976987 ID=Msa0138190-mRNA-1:exon:24642;Parent=Msa0138190-mRNA-1
chr1_3 CDS 70974376 70974464 70974376 ID=Msa0138190-mRNA-1:cds;Parent=Msa0138190-mRNA-1
chr1_3 CDS 70974583 70974753 70974583 ID=Msa0138190-mRNA-1:cds;Parent=Msa0138190-mRNA-1
chr1_3 CDS 70974854 70974932 70974854 ID=Msa0138190-mRNA-1:cds;Parent=Msa0138190-mRNA-1
chr1_3 CDS 70975045 70975116 70975045 ID=Msa0138190-mRNA-1:cds;Parent=Msa0138190-mRNA-1
chr1_3 CDS 70975669 70976559 70975669 ID=Msa0138190-mRNA-1:cds;Parent=Msa0138190-mRNA-1
chr1_3 CDS 70976695 70976871 70976695 ID=Msa0138190-mRNA-1:cds;Parent=Msa0138190-mRNA-1
chr1_3 CDS 70976987 70977094 70976987 ID=Msa0138190-mRNA-1:cds;Parent=Msa0138190-mRNA-1
Gene Sequence

>Msa0138190

ATGCAAAACTTTACTGAGAGATTAAGACCTCTTGTTGGTTTGAATGGCTGGGATTACTGTGTCTACTGGAAATTAAGTGAAGATCAAAGGTATCTTGAATGGTTGGGATGTTGTTGTGGTGGGAGTGAAAGCAACCAAAATGGTGGAGAAGAACACCTTTTCCCTGTCTCTTCATGTAGGGATACAATGTTTTCACACCCAAGAACAAACTCTTGTGATCTTCTTTCCCAACTTTCCACTTCCATACCTATAGATTCAGGGATTCATGCACAGGCTCTATTAACTAACCAACCCAATTGGTTGAACTATCCCAATAGCTTGGATCCAAACACTTTACATGAAACAATTAATGGGACCCATGTTTTGATTCCAGTGCCTGGTGGACTGGTTGAGCTCTTTGTAACTAAACAGGTTGGTGAAGATCAAAATGTAATAGATTTTATCACATCTCAATGCATTGTCATGGTGGATCAAGAAGGGATGAACCACTCAATTGGTTTCAATTCAATGAGCAACATGCAATCGAATCCAATTGTGGGTGATGAAAATGATGGAAATAATACAAACAATATTTTCCATTCATCAGACACATTGAATCTTCCACATGACATATCTGTATTGAACTTCATGCAACAGTTCAATTACAGTTACAATCAACAACAAAACAGAATCAAGAACAACGAAACCTTCTCTGAAGGATACCAAGGTTCTTTTCTTCATGATCAAGAAAACAACAACACTCTCAAATCCAACACAGAAGAGGTGCATGATAACAAAAGCTTGATGACAGATAATAATCAATATATGTTTGTGGACCCATTAGACACAAAAAAGAAGCAAGAACATGTTGTTGGTAGAGCAGATTCTATGTCAGATTGCAGTGATCAGAATGAAGAAGAGGATGATGGAAAATATAGGAGAAGAAGTGGAAAAGGAAACCAATCAAAGAACCTTGTTGCTGAGAGGAAGAGAAGGAAGAAACTGAATGATAGGTTATATAACCTTCGTTCATTGGTTCCAAGAATCTCAAAGCTTGATAGAGCTTCTATTCTTGGAGATGCTATTGAATTTGTGAAGGATTTGCAGAAGCAAGTGAAAGAGCTTCAAGATGAACTTGAGGAAAATTCAGACACAGGAACTGAAAGTAACTGTGTATATGTTGATGGTGTTCAAGTTCAACCAAATAATAATAACAATGTTCCAAAAGCAGAACATGGATTTCATGTTGGGAATGGCTATGTTTCAAAACAAAAGCAGGAAGATGTTGGTACTGTTACTGACAAGCAGACACAGCAGATGGAGGCACAAGTGGAAGTGGCTCAGATAGATGGGAATGAGTACTTTGTGAAGGTGTTTTGTGAGCACAGGCCAGGTGGTTTTGTGAAATTGATGGAGGCTTTGAACACTTTAGGCATGGATGTAATGCATGCCACAGTAACCAGTCACAAGGGTCTTGTCTCAAATGTTTTTAAAGTTGAGAATTATGAACAGAAAAAGGAGAGTGAAATAGTTGAGGCTGAAGAAGTTAGAGATTCACTACTAGAGCTGACACGGAACAAATCTTCATGCTGGAACTTGGAATCATGA
Protein sequence

>Msa0138190

MQNFTERLRPLVGLNGWDYCVYWKLSEDQRYLEWLGCCCGGSESNQNGGEEHLFPVSSCRDTMFSHPRTNSCDLLSQLSTSIPIDSGIHAQALLTNQPNWLNYPNSLDPNTLHETINGTHVLIPVPGGLVELFVTKQVGEDQNVIDFITSQCIVMVDQEGMNHSIGFNSMSNMQSNPIVGDENDGNNTNNIFHSSDTLNLPHDISVLNFMQQFNYSYNQQQNRIKNNETFSEGYQGSFLHDQENNNTLKSNTEEVHDNKSLMTDNNQYMFVDPLDTKKKQEHVVGRADSMSDCSDQNEEEDDGKYRRRSGKGNQSKNLVAERKRRKKLNDRLYNLRSLVPRISKLDRASILGDAIEFVKDLQKQVKELQDELEENSDTGTESNCVYVDGVQVQPNNNNNVPKAEHGFHVGNGYVSKQKQEDVGTVTDKQTQQMEAQVEVAQIDGNEYFVKVFCEHRPGGFVKLMEALNTLGMDVMHATVTSHKGLVSNVFKVENYEQKKESEIVEAEEVRDSLLELTRNKSSCWNLES*