AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0183440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0183440 MtrunA17_Chr1g0211081 94.253 522 21 4 1 521 6 519 0.0 1000
Msa0183440 MtrunA17_Chr8g0365711 28.872 523 256 13 5 518 4 419 1.30e-60 205
Msa0183440 MtrunA17_Chr3g0131691 34.146 205 114 2 311 515 293 476 2.81e-32 129
Msa0183440 MtrunA17_Chr7g0252041 36.275 204 106 4 315 515 285 467 1.05e-30 124
Msa0183440 MtrunA17_Chr2g0298751 34.715 193 107 5 309 498 45 221 2.99e-22 95.9
Msa0183440 MtrunA17_Chr4g0048851 33.529 170 81 3 315 484 46 183 3.38e-20 89.0
Msa0183440 MtrunA17_Chr2g0320291 31.034 174 81 4 301 471 121 258 6.02e-18 84.7
Msa0183440 MtrunA17_Chr4g0028071 31.176 170 70 5 310 467 143 277 2.39e-17 83.6
Msa0183440 MtrunA17_Chr3g0141091 27.962 211 108 4 304 514 161 327 5.83e-16 79.3
Msa0183440 MtrunA17_Chr1g0185621 28.972 214 118 6 310 513 464 653 3.59e-15 78.6
Msa0183440 MtrunA17_Chr8g0346161 30.180 222 113 9 295 506 268 457 6.88e-15 77.0
Msa0183440 MtrunA17_Chr5g0411541 29.050 179 91 3 304 482 144 286 8.69e-15 75.5
Msa0183440 MtrunA17_Chr5g0400661 29.570 186 110 7 309 493 142 307 8.75e-14 72.8
Msa0183440 MtrunA17_Chr7g0260841 42.045 88 50 1 284 371 324 410 1.48e-13 73.2
Msa0183440 MtrunA17_Chr4g0054581 26.012 173 104 4 308 479 190 339 3.10e-13 71.6
Msa0183440 MtrunA17_Chr8g0386921 29.630 189 110 6 296 473 386 562 3.42e-13 72.0
Msa0183440 MtrunA17_Chr7g0225901 27.528 178 102 4 302 479 74 224 4.98e-13 69.3
Msa0183440 MtrunA17_Chr5g0411341 27.679 224 126 8 300 513 472 669 7.89e-13 71.2
Msa0183440 MtrunA17_Chr7g0275821 55.556 54 24 0 320 373 256 309 1.52e-12 69.7
Msa0183440 MtrunA17_Chr8g0366751 29.268 164 94 4 320 480 466 610 5.03e-12 68.6
Msa0183440 MtrunA17_Chr8g0346151 40.260 77 44 1 295 369 256 332 6.68e-12 67.8
Msa0183440 MtrunA17_Chr5g0400671 33.000 100 60 1 288 380 119 218 1.18e-11 66.2
Msa0183440 MtrunA17_Chr5g0400621 42.222 90 47 3 303 392 145 229 1.84e-11 65.5
Msa0183440 MtrunA17_Chr2g0307131 27.168 173 99 4 307 479 170 315 1.93e-11 65.9
Msa0183440 MtrunA17_Chr4g0048891 37.624 101 57 2 279 373 2 102 1.95e-11 62.0
Msa0183440 MtrunA17_Chr4g0049331 49.091 55 28 0 317 371 136 190 2.94e-11 64.7
Msa0183440 MtrunA17_Chr6g0468491 29.630 189 99 8 294 479 116 273 3.01e-11 64.7
Msa0183440 MtrunA17_Chr4g0054571 36.111 72 46 0 300 371 134 205 6.47e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0183440 AT2G16910.1 46.898 548 228 17 1 524 5 513 1.22e-145 431
Msa0183440 AT3G26744.1 35.979 189 98 2 315 500 306 474 8.67e-31 125
Msa0183440 AT3G26744.4 35.979 189 98 2 315 500 306 474 8.67e-31 125
Msa0183440 AT3G26744.2 35.979 189 98 2 315 500 306 474 8.67e-31 125
Msa0183440 AT3G26744.6 36.813 182 92 2 315 493 306 467 2.90e-30 124
Msa0183440 AT1G12860.1 35.821 201 105 4 315 515 266 442 9.34e-30 122
Msa0183440 AT1G12860.2 36.313 179 93 2 315 493 266 423 7.18e-29 119
Msa0183440 AT1G10610.1 35.404 161 88 4 2 152 8 162 9.55e-25 107
Msa0183440 AT1G10610.1 25.962 208 116 4 314 516 262 436 1.05e-15 80.1
Msa0183440 AT5G57150.1 32.212 208 117 5 298 500 32 220 2.29e-20 90.9
Msa0183440 AT5G57150.5 32.338 201 112 5 298 493 32 213 5.13e-20 89.4
Msa0183440 AT5G57150.3 32.338 201 112 5 298 493 33 214 7.07e-20 89.0
Msa0183440 AT5G57150.2 32.338 201 112 5 298 493 33 214 7.66e-20 89.0
Msa0183440 AT5G57150.4 32.338 201 112 5 298 493 33 214 1.10e-19 89.4
Msa0183440 AT2G28160.2 30.061 163 89 4 308 470 123 260 5.83e-17 82.4
Msa0183440 AT4G21330.1 28.155 206 118 4 314 519 30 205 5.97e-17 80.1
Msa0183440 AT2G28160.1 30.061 163 89 4 308 470 123 260 7.73e-17 82.0
Msa0183440 AT1G10610.2 32.836 134 75 3 28 152 5 132 1.08e-16 82.8
Msa0183440 AT1G10610.2 25.962 208 116 4 314 516 232 406 8.76e-16 80.1
Msa0183440 AT5G46760.1 31.677 161 87 4 320 480 419 556 2.14e-16 82.8
Msa0183440 AT5G65640.1 28.959 221 122 5 304 524 166 351 5.03e-16 80.1
Msa0183440 AT1G10610.3 26.486 185 103 3 314 493 165 321 6.09e-16 79.7
Msa0183440 AT1G63650.3 30.814 172 102 4 319 486 408 566 6.73e-16 81.3
Msa0183440 AT1G63650.1 30.814 172 102 4 319 486 408 566 6.73e-16 81.3
Msa0183440 AT1G63650.2 30.814 172 102 4 319 486 408 566 6.73e-16 81.3
Msa0183440 AT4G29930.1 30.928 194 124 5 315 507 53 237 8.10e-16 77.8
Msa0183440 AT2G46510.1 44.086 93 47 1 301 388 375 467 9.61e-16 80.5
Msa0183440 AT5G65640.2 57.746 71 30 0 304 374 166 236 6.24e-15 75.9
Msa0183440 AT4G17880.1 29.570 186 99 5 300 480 395 553 6.84e-15 77.8
Msa0183440 AT5G10570.1 29.717 212 103 6 306 512 138 308 7.69e-15 75.9
Msa0183440 AT4G29930.3 30.508 177 115 4 315 490 53 222 8.39e-15 75.1
Msa0183440 AT4G29930.7 30.508 177 115 4 315 490 53 222 8.39e-15 75.1
Msa0183440 AT4G29930.4 55.000 60 27 0 315 374 53 112 4.59e-14 71.2
Msa0183440 AT4G29930.2 55.000 60 27 0 315 374 53 112 5.79e-14 72.0
Msa0183440 AT4G29930.6 55.000 60 27 0 315 374 53 112 6.15e-14 71.6
Msa0183440 AT4G29930.5 55.000 60 27 0 315 374 53 112 6.18e-14 72.0
Msa0183440 AT5G46830.1 28.718 195 105 6 320 514 347 507 1.05e-12 70.9
Msa0183440 AT4G37850.2 27.059 170 97 4 311 479 147 290 1.20e-12 69.7
Msa0183440 AT4G37850.1 27.059 170 97 4 311 479 147 290 1.20e-12 69.7
Msa0183440 AT5G41315.1 28.877 187 110 7 308 486 436 607 1.90e-12 70.5
Msa0183440 AT5G41315.3 28.877 187 110 7 308 486 441 612 1.93e-12 70.5
Msa0183440 AT1G01260.1 42.045 88 49 2 287 373 404 490 2.01e-12 70.1
Msa0183440 AT1G01260.2 42.045 88 49 2 287 373 404 490 2.01e-12 70.1
Msa0183440 AT1G01260.3 42.045 88 49 2 287 373 404 490 2.01e-12 70.1
Msa0183440 AT5G41315.2 28.977 176 105 6 319 486 452 615 2.08e-12 70.1
Msa0183440 AT4G09820.1 26.786 168 74 3 317 482 364 484 3.98e-12 68.9
Msa0183440 AT4G16430.1 43.421 76 38 1 301 371 300 375 6.54e-12 68.2
Msa0183440 AT1G32640.1 28.205 195 109 4 320 514 456 619 1.06e-11 67.8
Msa0183440 AT2G22750.4 26.471 170 103 3 310 479 148 295 6.17e-11 64.3
Msa0183440 AT2G22750.5 26.471 170 103 3 310 479 144 291 6.27e-11 64.3
Msa0183440 AT2G22750.3 26.471 170 103 3 310 479 148 295 6.27e-11 64.3
Msa0183440 AT4G00870.1 40.323 62 37 0 320 381 253 314 7.98e-11 64.7
Msa0183440 AT2G22750.1 26.471 170 103 3 310 479 120 267 8.85e-11 63.5
Msa0183440 AT2G22750.2 26.471 170 103 3 310 479 120 267 9.01e-11 63.5

Find 106 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAACATTGTTATTATTATT+TGG 0.155242 1_4:+72375181 None:intergenic
CTATCAAGCTTTGAGATTCT+TGG 0.205780 1_4:+72375343 None:intergenic
CTCTTGTTGGTTTGAATGGC+TGG 0.239275 1_4:-72377207 Msa0183440:CDS
TGAAATCCATGTTCTGCTTT+TGG 0.239465 1_4:+72375160 None:intergenic
CTTGATAGAGCATCTATTCT+TGG 0.248227 1_4:-72375328 Msa0183440:CDS
ATAGAATTATGAACAGAAAA+AGG 0.296948 1_4:-72374635 Msa0183440:intron
CAGCAACAAGGTTCTTTGAT+TGG 0.308174 1_4:+72375422 None:intergenic
GTGGAAGTGGCTCAGATAGA+TGG 0.338551 1_4:-72374917 Msa0183440:CDS
GTTATATAACCTTCGTTCAT+TGG 0.349149 1_4:-72375368 Msa0183440:CDS
AGATCACAAGAGTTTGTTCT+TGG 0.351468 1_4:+72376921 None:intergenic
GGTGTTCTTCTCCACCATTT+TGG 0.356060 1_4:+72376985 None:intergenic
GAACTTACATGTAAAGTGTT+TGG 0.359394 1_4:+72376700 None:intergenic
GAAGGAAGAAACTGAATGAT+AGG 0.368436 1_4:-72375390 Msa0183440:CDS
CAAATCTTCATGCTGGAACT+TGG 0.371640 1_4:-72374554 Msa0183440:CDS
AGACCTCTTGTTGGTTTGAA+TGG 0.376737 1_4:-72377211 Msa0183440:CDS
GTGTTTGGATCCAAGCTATT+GGG 0.381111 1_4:+72376715 None:intergenic
TAGGTATGGAAGTGGAAAGT+TGG 0.382059 1_4:+72376892 None:intergenic
ATTGGGATAGTTCAACCAAT+TGG 0.386734 1_4:+72376732 None:intergenic
GATTCCAGTGCCTGGTGGAC+TGG 0.406904 1_4:-72376547 Msa0183440:CDS
CAGAACATGGATTTCATGTT+GGG 0.411727 1_4:-72375153 Msa0183440:CDS
TATATATACCCTGAATCTAT+AGG 0.413784 1_4:+72376873 None:intergenic
TGAGCTCTTTGTAACTAAAC+AGG 0.419409 1_4:-72376523 Msa0183440:intron
GGTTGGGATGTTGTTGTGGT+GGG 0.420257 1_4:-72377019 Msa0183440:CDS
AAGAGGATGATGGAAAATAT+AGG 0.425274 1_4:-72375465 Msa0183440:CDS
AGTGTTTGGATCCAAGCTAT+TGG 0.426318 1_4:+72376714 None:intergenic
ACTTCCATACCTATAGATTC+AGG 0.426419 1_4:-72376882 Msa0183440:CDS
TTTCCCTGTCTCTTCATGTA+GGG 0.439137 1_4:-72376961 Msa0183440:CDS
TTGGGATAGTTCAACCAATT+GGG 0.440118 1_4:+72376733 None:intergenic
TATTAACTAACCAACCCAAT+TGG 0.442270 1_4:-72376747 Msa0183440:CDS
TATAGGAGAAGAAGTGGAAA+AGG 0.445604 1_4:-72375448 Msa0183440:CDS
AGGTATGGAAGTGGAAAGTT+GGG 0.445933 1_4:+72376893 None:intergenic
GAAGGGATGAACCACTCAAT+TGG 0.446434 1_4:-72375886 Msa0183440:CDS
GAGAGATTAAGACCTCTTGT+TGG 0.450531 1_4:-72377220 Msa0183440:CDS
ATGGAGGCTTTGAACACTTT+AGG 0.460171 1_4:-72374836 Msa0183440:CDS
TTATATATAGGTATCTTGAA+TGG 0.460510 1_4:-72377040 Msa0183440:intron
AGTAACTGTGTATATGTTGA+TGG 0.466614 1_4:-72375217 Msa0183440:CDS
TGGTTGGGATGTTGTTGTGG+TGG 0.468844 1_4:-72377020 Msa0183440:CDS
ATATAGGTATCTTGAATGGT+TGG 0.468983 1_4:-72377036 Msa0183440:intron
GCAGAACATGGATTTCATGT+TGG 0.469960 1_4:-72375154 Msa0183440:CDS
GGAAAATATAGGAGAAGAAG+TGG 0.472548 1_4:-72375454 Msa0183440:CDS
GATCACAAGAGTTTGTTCTT+GGG 0.473126 1_4:+72376922 None:intergenic
TCTTGTTGGTTTGAATGGCT+GGG 0.473478 1_4:-72377206 Msa0183440:CDS
GATTCAATGTCTCTGATGAA+TGG 0.473958 1_4:+72375785 None:intergenic
AAGAAGCAAGAACATGTTGT+TGG 0.475916 1_4:-72375529 Msa0183440:CDS
GCTGGGATTACTGTGTCTAC+TGG 0.476054 1_4:-72377189 Msa0183440:CDS
GGCTTTGAACACTTTAGGCA+TGG 0.486692 1_4:-72374831 Msa0183440:CDS
ATGAATATTGCAGGCACAAG+TGG 0.489997 1_4:-72374936 Msa0183440:intron
TATAGGTATCTTGAATGGTT+GGG 0.493698 1_4:-72377035 Msa0183440:intron
TAATAATCAATATATGTTTG+TGG 0.498103 1_4:-72375569 Msa0183440:CDS
GTTGAACTATCCCAATAGCT+TGG 0.501147 1_4:-72376725 Msa0183440:CDS
CAGAATGAAGAAGAGGATGA+TGG 0.501916 1_4:-72375475 Msa0183440:CDS
ATTGAACAGGATTCATGCAC+AGG 0.504490 1_4:-72376773 Msa0183440:intron
TTCTCTTCCTCTCAGCAACA+AGG 0.506548 1_4:+72375410 None:intergenic
GAATGGTTGGGATGTTGTTG+TGG 0.510919 1_4:-72377023 Msa0183440:CDS
GTATCCCTACATGAAGAGAC+AGG 0.511754 1_4:+72376957 None:intergenic
ATACCCTGAATCTATAGGTA+TGG 0.513797 1_4:+72376878 None:intergenic
GGGAGTGAAAGCAACCAAAA+TGG 0.520448 1_4:-72376999 Msa0183440:CDS
TGGAAGTGGCTCAGATAGAT+GGG 0.522256 1_4:-72374916 Msa0183440:CDS
TGAGACAAGACCCTTGTGAC+TGG 0.522381 1_4:+72374787 None:intergenic
CATGGATTTCATGTTGGGAA+TGG 0.525371 1_4:-72375148 Msa0183440:CDS
ATTGCAGGCACAAGTGGAAG+TGG 0.525672 1_4:-72374930 Msa0183440:intron
TGAATCTATAGGTATGGAAG+TGG 0.528440 1_4:+72376884 None:intergenic
CACGGAACAAATCTTCATGC+TGG 0.531543 1_4:-72374561 Msa0183440:CDS
CTTCCATACCTATAGATTCA+GGG 0.537250 1_4:-72376881 Msa0183440:intron
ATGCAATCGAATCCAATTGT+GGG 0.539218 1_4:-72375844 Msa0183440:CDS
ACCTTGGTATTCTTCAGAGA+AGG 0.541843 1_4:+72375675 None:intergenic
CTATGTTTCAAAACAAAAGC+AGG 0.545019 1_4:-72375125 Msa0183440:CDS
GGAAATTAAGTGAAGATCAA+AGG 0.546606 1_4:-72377168 Msa0183440:intron
CATGCAATCGAATCCAATTG+TGG 0.546804 1_4:-72375845 Msa0183440:CDS
GATAGTTCAACCAATTGGGT+TGG 0.554141 1_4:+72376737 None:intergenic
TACATGAAGAGACAGGGAAA+AGG 0.554895 1_4:+72376964 None:intergenic
TTGAATTGAAACCAATTGAG+TGG 0.557955 1_4:+72375875 None:intergenic
CAGGCACTGGAATCAAAACA+TGG 0.559119 1_4:+72376556 None:intergenic
CAGTGATCAGAATGAAGAAG+AGG 0.559858 1_4:-72375482 Msa0183440:CDS
TTGTTGCTGAGAGGAAGAGA+AGG 0.562094 1_4:-72375408 Msa0183440:CDS
AAGAGCTCAACCAGTCCACC+AGG 0.565516 1_4:+72376537 None:intergenic
ATCAATTTCACAAAACCACC+TGG 0.575508 1_4:+72374857 None:intergenic
TCAACCAGTCCACCAGGCAC+TGG 0.579883 1_4:+72376543 None:intergenic
GATCCTGAAGAAAAGAACCT+TGG 0.582504 1_4:+72375659 None:intergenic
AAACAAAAGCAGGAAGATGT+TGG 0.583119 1_4:-72375115 Msa0183440:CDS
ATCTCAATGCATTGTCATGG+TGG 0.583356 1_4:-72375914 Msa0183440:CDS
TGACAAGCAGACACAGCAGA+TGG 0.586592 1_4:-72375083 Msa0183440:CDS
AAAGGAGAGTGAAATAGTTG+AGG 0.586665 1_4:-72374617 Msa0183440:CDS
CACATCTCAATGCATTGTCA+TGG 0.588350 1_4:-72375917 Msa0183440:CDS
TTCAATACAGATATGTCATG+TGG 0.598259 1_4:+72375760 None:intergenic
AGAGCTTCAAGATGAACTTG+AGG 0.601650 1_4:-72375263 Msa0183440:CDS
AATGTTCCAAAAGCAGAACA+TGG 0.606756 1_4:-72375166 Msa0183440:CDS
TCTCAAATCACACACAGAAG+AGG 0.606938 1_4:-72375620 Msa0183440:CDS
ATTGTCATGGTGGATCAAGA+AGG 0.607678 1_4:-72375904 Msa0183440:CDS
CAAAGAACCTTGTTGCTGAG+AGG 0.619036 1_4:-72375417 Msa0183440:CDS
CTTGAGGAAAATTCAGACAC+AGG 0.625559 1_4:-72375247 Msa0183440:CDS
TGCCACAGTAACCAGTCACA+AGG 0.625627 1_4:-72374798 Msa0183440:CDS
AGATGCTATTGAATTTGTGA+AGG 0.628469 1_4:-72375305 Msa0183440:CDS
CAGCCATTCAAACCAACAAG+AGG 0.632565 1_4:+72377208 None:intergenic
ATTGTGGGTGATGAAAATGA+TGG 0.641485 1_4:-72375829 Msa0183440:CDS
AGTGAAAGCAACCAAAATGG+TGG 0.646392 1_4:-72376996 Msa0183440:CDS
ATTCACTACTAGAGCTGACA+CGG 0.647595 1_4:-72374579 Msa0183440:CDS
TTCTTGGAACCAATGAACGA+AGG 0.654816 1_4:+72375359 None:intergenic
ACCCTTGTGACTGGTTACTG+TGG 0.657104 1_4:+72374796 None:intergenic
TTGTCATGGTGGATCAAGAA+GGG 0.669108 1_4:-72375903 Msa0183440:CDS
TGGGAATGAGTACTTTGTGA+AGG 0.678653 1_4:-72374897 Msa0183440:CDS
CAAGCAGACACAGCAGATGG+AGG 0.682677 1_4:-72375080 Msa0183440:intron
TATCCCTACATGAAGAGACA+GGG 0.687876 1_4:+72376958 None:intergenic
ACCTTCTCTGAAGAATACCA+AGG 0.692673 1_4:-72375676 Msa0183440:CDS
GCCACAGTAACCAGTCACAA+GGG 0.696830 1_4:-72374797 Msa0183440:CDS
AGGCACTGGAATCAAAACAT+GGG 0.706180 1_4:+72376557 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAATAGAACAAAAAATA+TGG + chr1_4:72374673-72374692 None:intergenic 10.0%
!!! TAAATTTTAAATTAAGCTAA+AGG + chr1_4:72375355-72375374 None:intergenic 10.0%
!! AGTTATTAGTATATTTATTT+AGG + chr1_4:72375790-72375809 None:intergenic 10.0%
!! AATTTATATACTAATAACAA+AGG - chr1_4:72376749-72376768 Msa0183440:CDS 10.0%
!!! ATGATATTAAATTTTTTTCT+TGG - chr1_4:72377114-72377133 Msa0183440:intron 10.0%
!!! TGATATTAAATTTTTTTCTT+GGG - chr1_4:72377115-72377134 Msa0183440:intron 10.0%
!!! ATTTGTTGGTTTTTATATAT+AGG - chr1_4:72374729-72374748 Msa0183440:intron 15.0%
!! AAGGATTATATATAGATAAA+GGG + chr1_4:72374978-72374997 None:intergenic 15.0%
!! AAAGGATTATATATAGATAA+AGG + chr1_4:72374979-72374998 None:intergenic 15.0%
!! AAAATAGTACTCAATATATA+TGG + chr1_4:72375183-72375202 None:intergenic 15.0%
!!! ACTTTTTTGAATTATTATCA+AGG - chr1_4:72375628-72375647 Msa0183440:CDS 15.0%
!! CATAATAAAAATATGAAGTT+CGG - chr1_4:72375661-72375680 Msa0183440:CDS 15.0%
!! TAATAATCAATATATGTTTG+TGG - chr1_4:72376212-72376231 Msa0183440:intron 15.0%
!!! TATAAATTTTAGTATAAGCT+AGG + chr1_4:72376737-72376756 None:intergenic 15.0%
!!! TAATTGTGATGTTAATTTGT+TGG - chr1_4:72374715-72374734 Msa0183440:intron 20.0%
!! TTATATATAGGTATCTTGAA+TGG - chr1_4:72374741-72374760 Msa0183440:intron 20.0%
!! ATTATATATAGATAAAGGGA+GGG + chr1_4:72374974-72374993 None:intergenic 20.0%
!! AGATGAATTAGTTGAAAAAT+AGG + chr1_4:72375122-72375141 None:intergenic 20.0%
!!! TTTTTCAACTAATTCATCTT+TGG - chr1_4:72375123-72375142 Msa0183440:CDS 20.0%
!! TATAAGTAGGTAGATATAAA+TGG - chr1_4:72375318-72375337 Msa0183440:CDS 20.0%
!! TCATTGAATGATATACTATA+AGG - chr1_4:72375470-72375489 Msa0183440:CDS 20.0%
!!! TAAGATCATGTTAATTTTGA+TGG - chr1_4:72375569-72375588 Msa0183440:CDS 20.0%
!!! TGAATTATTATCAAGGTTTT+TGG - chr1_4:72375635-72375654 Msa0183440:CDS 20.0%
!!! GGAACATTGTTATTATTATT+TGG + chr1_4:72376603-72376622 None:intergenic 20.0%
!! AAGTTAAGAAAATGATTCTA+AGG + chr1_4:72377045-72377064 None:intergenic 20.0%
!! AGACAAATTCAAATCAAAAT+AGG - chr1_4:72377081-72377100 Msa0183440:intron 20.0%
!! ATAGAATTATGAACAGAAAA+AGG - chr1_4:72377146-72377165 Msa0183440:intron 20.0%
! TATATATACCCTGAATCTAT+AGG + chr1_4:72374911-72374930 None:intergenic 25.0%
! GATTATATATAGATAAAGGG+AGG + chr1_4:72374975-72374994 None:intergenic 25.0%
! TATATGGAATTAGCTGAAAA+AGG + chr1_4:72375167-72375186 None:intergenic 25.0%
!!! ATATTGAGTACTATTTTTGC+AGG - chr1_4:72375186-72375205 Msa0183440:CDS 25.0%
!! TTTTTGCAGGAAACAATTAA+TGG - chr1_4:72375199-72375218 Msa0183440:CDS 25.0%
!! TTTTGCAGGAAACAATTAAT+GGG - chr1_4:72375200-72375219 Msa0183440:CDS 25.0%
! TTAGAAACACACATATAAGT+AGG - chr1_4:72375305-72375324 Msa0183440:CDS 25.0%
! GCTTAATTTACATATTGAAC+TGG - chr1_4:72375410-72375429 Msa0183440:CDS 25.0%
! TTCTCTGTGTATAATATAAC+AGG - chr1_4:72375810-72375829 Msa0183440:CDS 25.0%
!!! CTCAAATGTTTTTAAAGTTG+AGG - chr1_4:72377013-72377032 Msa0183440:CDS 25.0%
GGAAATTAAGTGAAGATCAA+AGG - chr1_4:72374613-72374632 Msa0183440:CDS 30.0%
ATATAGGTATCTTGAATGGT+TGG - chr1_4:72374745-72374764 Msa0183440:intron 30.0%
TATAGGTATCTTGAATGGTT+GGG - chr1_4:72374746-72374765 Msa0183440:intron 30.0%
CTGTTCAATGAAGAGATTAA+AGG + chr1_4:72374997-72375016 None:intergenic 30.0%
CTTTAATCTCTTCATTGAAC+AGG - chr1_4:72374995-72375014 Msa0183440:intron 30.0%
TATTAACTAACCAACCCAAT+TGG - chr1_4:72375034-72375053 Msa0183440:intron 30.0%
! GAACTTACATGTAAAGTGTT+TGG + chr1_4:72375084-72375103 None:intergenic 30.0%
CACTTTACATGTAAGTTCAT+TGG - chr1_4:72375086-72375105 Msa0183440:CDS 30.0%
GGTCTAATTATGATGTTGAA+GGG + chr1_4:72375505-72375524 None:intergenic 30.0%
TGATCTTGATGTTGTTAACT+GGG + chr1_4:72375528-72375547 None:intergenic 30.0%
!! TATAAAAGCGTCAAAGATGT+TGG + chr1_4:72375688-72375707 None:intergenic 30.0%
CTGTGTATAATATAACAGGT+TGG - chr1_4:72375814-72375833 Msa0183440:CDS 30.0%
TTGAATTGAAACCAATTGAG+TGG + chr1_4:72375909-72375928 None:intergenic 30.0%
TTCAATACAGATATGTCATG+TGG + chr1_4:72376024-72376043 None:intergenic 30.0%
!!! GCTTCTTTTTTGTGTCTAAT+GGG + chr1_4:72376239-72376258 None:intergenic 30.0%
!!! TGCTTCTTTTTTGTGTCTAA+TGG + chr1_4:72376240-72376259 None:intergenic 30.0%
!! AAGAGGATGATGGAAAATAT+AGG - chr1_4:72376316-72376335 Msa0183440:intron 30.0%
GTTATATAACCTTCGTTCAT+TGG - chr1_4:72376413-72376432 Msa0183440:intron 30.0%
AGATGCTATTGAATTTGTGA+AGG - chr1_4:72376476-72376495 Msa0183440:intron 30.0%
AGTAACTGTGTATATGTTGA+TGG - chr1_4:72376564-72376583 Msa0183440:CDS 30.0%
CTATGTTTCAAAACAAAAGC+AGG - chr1_4:72376656-72376675 Msa0183440:intron 30.0%
CACATGAATATGAATATTGC+AGG - chr1_4:72376836-72376855 Msa0183440:intron 30.0%
! GATCACAAGAGTTTGTTCTT+GGG + chr1_4:72374862-72374881 None:intergenic 35.0%
! AGATCACAAGAGTTTGTTCT+TGG + chr1_4:72374863-72374882 None:intergenic 35.0%
TGAATCTATAGGTATGGAAG+TGG + chr1_4:72374900-72374919 None:intergenic 35.0%
ACTTCCATACCTATAGATTC+AGG - chr1_4:72374899-72374918 Msa0183440:CDS 35.0%
CTTCCATACCTATAGATTCA+GGG - chr1_4:72374900-72374919 Msa0183440:CDS 35.0%
ATACCCTGAATCTATAGGTA+TGG + chr1_4:72374906-72374925 None:intergenic 35.0%
TTGGGATAGTTCAACCAATT+GGG + chr1_4:72375051-72375070 None:intergenic 35.0%
ATTGGGATAGTTCAACCAAT+TGG + chr1_4:72375052-72375071 None:intergenic 35.0%
TGAGCTCTTTGTAACTAAAC+AGG - chr1_4:72375258-72375277 Msa0183440:CDS 35.0%
GGGTCTAATTATGATGTTGA+AGG + chr1_4:72375506-72375525 None:intergenic 35.0%
ATCTTGATGTTGTTAACTGG+GGG + chr1_4:72375526-72375545 None:intergenic 35.0%
GATCTTGATGTTGTTAACTG+GGG + chr1_4:72375527-72375546 None:intergenic 35.0%
GTGATCTTGATGTTGTTAAC+TGG + chr1_4:72375529-72375548 None:intergenic 35.0%
TTTAGGCAAAACTGCATCAA+AGG + chr1_4:72375773-72375792 None:intergenic 35.0%
ATGCAATCGAATCCAATTGT+GGG - chr1_4:72375937-72375956 Msa0183440:CDS 35.0%
! TCATTTTCATCACCCACAAT+TGG + chr1_4:72375952-72375971 None:intergenic 35.0%
ATTGTGGGTGATGAAAATGA+TGG - chr1_4:72375952-72375971 Msa0183440:CDS 35.0%
GATTCAATGTCTCTGATGAA+TGG + chr1_4:72375999-72376018 None:intergenic 35.0%
! ATACCAAGGTTCTTTTCTTC+AGG - chr1_4:72376119-72376138 Msa0183440:intron 35.0%
AAGAAGCAAGAACATGTTGT+TGG - chr1_4:72376252-72376271 Msa0183440:intron 35.0%
GGAAAATATAGGAGAAGAAG+TGG - chr1_4:72376327-72376346 Msa0183440:intron 35.0%
TATAGGAGAAGAAGTGGAAA+AGG - chr1_4:72376333-72376352 Msa0183440:intron 35.0%
GAAGGAAGAAACTGAATGAT+AGG - chr1_4:72376391-72376410 Msa0183440:intron 35.0%
!! CTATCAAGCTTTGAGATTCT+TGG + chr1_4:72376441-72376460 None:intergenic 35.0%
! CTTGATAGAGCATCTATTCT+TGG - chr1_4:72376453-72376472 Msa0183440:intron 35.0%
AATGTTCCAAAAGCAGAACA+TGG - chr1_4:72376615-72376634 Msa0183440:intron 35.0%
! TGAAATCCATGTTCTGCTTT+TGG + chr1_4:72376624-72376643 None:intergenic 35.0%
CAGAACATGGATTTCATGTT+GGG - chr1_4:72376628-72376647 Msa0183440:intron 35.0%
AAACAAAAGCAGGAAGATGT+TGG - chr1_4:72376666-72376685 Msa0183440:intron 35.0%
ATCAATTTCACAAAACCACC+TGG + chr1_4:72376927-72376946 None:intergenic 35.0%
!!! AGGTGGTTTTGTGAAATTGA+TGG - chr1_4:72376926-72376945 Msa0183440:CDS 35.0%
!!! TGGTTTTGTGAAATTGATGG+AGG - chr1_4:72376929-72376948 Msa0183440:CDS 35.0%
AAAGGAGAGTGAAATAGTTG+AGG - chr1_4:72377164-72377183 Msa0183440:intron 35.0%
GAGAGATTAAGACCTCTTGT+TGG - chr1_4:72374561-72374580 Msa0183440:CDS 40.0%
!! AGACCTCTTGTTGGTTTGAA+TGG - chr1_4:72374570-72374589 Msa0183440:CDS 40.0%
!! TCTTGTTGGTTTGAATGGCT+GGG - chr1_4:72374575-72374594 Msa0183440:CDS 40.0%
AGTGAAAGCAACCAAAATGG+TGG - chr1_4:72374785-72374804 Msa0183440:CDS 40.0%
TACATGAAGAGACAGGGAAA+AGG + chr1_4:72374820-72374839 None:intergenic 40.0%
! TTTTCCCTGTCTCTTCATGT+AGG - chr1_4:72374819-72374838 Msa0183440:CDS 40.0%
TTTCCCTGTCTCTTCATGTA+GGG - chr1_4:72374820-72374839 Msa0183440:CDS 40.0%
TATCCCTACATGAAGAGACA+GGG + chr1_4:72374826-72374845 None:intergenic 40.0%
AGGTATGGAAGTGGAAAGTT+GGG + chr1_4:72374891-72374910 None:intergenic 40.0%
TAGGTATGGAAGTGGAAAGT+TGG + chr1_4:72374892-72374911 None:intergenic 40.0%
ATTGAACAGGATTCATGCAC+AGG - chr1_4:72375008-72375027 Msa0183440:intron 40.0%
GATAGTTCAACCAATTGGGT+TGG + chr1_4:72375047-72375066 None:intergenic 40.0%
GTTGAACTATCCCAATAGCT+TGG - chr1_4:72375056-72375075 Msa0183440:intron 40.0%
GTGTTTGGATCCAAGCTATT+GGG + chr1_4:72375069-72375088 None:intergenic 40.0%
AGTGTTTGGATCCAAGCTAT+TGG + chr1_4:72375070-72375089 None:intergenic 40.0%
!! AGGCACTGGAATCAAAACAT+GGG + chr1_4:72375227-72375246 None:intergenic 40.0%
CACATCTCAATGCATTGTCA+TGG - chr1_4:72375864-72375883 Msa0183440:CDS 40.0%
ATCTCAATGCATTGTCATGG+TGG - chr1_4:72375867-72375886 Msa0183440:CDS 40.0%
!! ATTGTCATGGTGGATCAAGA+AGG - chr1_4:72375877-72375896 Msa0183440:CDS 40.0%
!! TTGTCATGGTGGATCAAGAA+GGG - chr1_4:72375878-72375897 Msa0183440:CDS 40.0%
CATGCAATCGAATCCAATTG+TGG - chr1_4:72375936-72375955 Msa0183440:CDS 40.0%
! ACCTTCTCTGAAGAATACCA+AGG - chr1_4:72376105-72376124 Msa0183440:intron 40.0%
ACCTTGGTATTCTTCAGAGA+AGG + chr1_4:72376109-72376128 None:intergenic 40.0%
GATCCTGAAGAAAAGAACCT+TGG + chr1_4:72376125-72376144 None:intergenic 40.0%
TCTCAAATCACACACAGAAG+AGG - chr1_4:72376161-72376180 Msa0183440:intron 40.0%
CAGTGATCAGAATGAAGAAG+AGG - chr1_4:72376299-72376318 Msa0183440:intron 40.0%
CAGAATGAAGAAGAGGATGA+TGG - chr1_4:72376306-72376325 Msa0183440:intron 40.0%
CAGCAACAAGGTTCTTTGAT+TGG + chr1_4:72376362-72376381 None:intergenic 40.0%
TTCTTGGAACCAATGAACGA+AGG + chr1_4:72376425-72376444 None:intergenic 40.0%
AGAGCTTCAAGATGAACTTG+AGG - chr1_4:72376518-72376537 Msa0183440:intron 40.0%
CTTGAGGAAAATTCAGACAC+AGG - chr1_4:72376534-72376553 Msa0183440:CDS 40.0%
GCAGAACATGGATTTCATGT+TGG - chr1_4:72376627-72376646 Msa0183440:intron 40.0%
CATGGATTTCATGTTGGGAA+TGG - chr1_4:72376633-72376652 Msa0183440:intron 40.0%
ATGAATATTGCAGGCACAAG+TGG - chr1_4:72376845-72376864 Msa0183440:intron 40.0%
TGGGAATGAGTACTTTGTGA+AGG - chr1_4:72376884-72376903 Msa0183440:CDS 40.0%
!! ATGGAGGCTTTGAACACTTT+AGG - chr1_4:72376945-72376964 Msa0183440:CDS 40.0%
ATTCACTACTAGAGCTGACA+CGG - chr1_4:72377202-72377221 Msa0183440:CDS 40.0%
CAAATCTTCATGCTGGAACT+TGG - chr1_4:72377227-72377246 Msa0183440:CDS 40.0%
CAGCCATTCAAACCAACAAG+AGG + chr1_4:72374576-72374595 None:intergenic 45.0%
!! CTCTTGTTGGTTTGAATGGC+TGG - chr1_4:72374574-72374593 Msa0183440:CDS 45.0%
GAATGGTTGGGATGTTGTTG+TGG - chr1_4:72374758-72374777 Msa0183440:intron 45.0%
GGGAGTGAAAGCAACCAAAA+TGG - chr1_4:72374782-72374801 Msa0183440:CDS 45.0%
! GGTGTTCTTCTCCACCATTT+TGG + chr1_4:72374799-72374818 None:intergenic 45.0%
GTATCCCTACATGAAGAGAC+AGG + chr1_4:72374827-72374846 None:intergenic 45.0%
!! CAGGCACTGGAATCAAAACA+TGG + chr1_4:72375228-72375247 None:intergenic 45.0%
!!! CATGTTTTGATTCCAGTGCC+TGG - chr1_4:72375226-72375245 Msa0183440:CDS 45.0%
GAAGGGATGAACCACTCAAT+TGG - chr1_4:72375895-72375914 Msa0183440:CDS 45.0%
CAAAGAACCTTGTTGCTGAG+AGG - chr1_4:72376364-72376383 Msa0183440:intron 45.0%
TTCTCTTCCTCTCAGCAACA+AGG + chr1_4:72376374-72376393 None:intergenic 45.0%
TTGTTGCTGAGAGGAAGAGA+AGG - chr1_4:72376373-72376392 Msa0183440:intron 45.0%
TGGAAGTGGCTCAGATAGAT+GGG - chr1_4:72376865-72376884 Msa0183440:intron 45.0%
!!! TGAAGGTGTTTTGTGAGCAC+AGG - chr1_4:72376901-72376920 Msa0183440:CDS 45.0%
!! GGCTTTGAACACTTTAGGCA+TGG - chr1_4:72376950-72376969 Msa0183440:CDS 45.0%
CACGGAACAAATCTTCATGC+TGG - chr1_4:72377220-72377239 Msa0183440:CDS 45.0%
GCTGGGATTACTGTGTCTAC+TGG - chr1_4:72374592-72374611 Msa0183440:CDS 50.0%
TGGTTGGGATGTTGTTGTGG+TGG - chr1_4:72374761-72374780 Msa0183440:intron 50.0%
GGTTGGGATGTTGTTGTGGT+GGG - chr1_4:72374762-72374781 Msa0183440:intron 50.0%
!!! GTTTTGATTCCAGTGCCTGG+TGG - chr1_4:72375229-72375248 Msa0183440:CDS 50.0%
TGACAAGCAGACACAGCAGA+TGG - chr1_4:72376698-72376717 Msa0183440:intron 50.0%
ATTGCAGGCACAAGTGGAAG+TGG - chr1_4:72376851-72376870 Msa0183440:intron 50.0%
GTGGAAGTGGCTCAGATAGA+TGG - chr1_4:72376864-72376883 Msa0183440:intron 50.0%
TGCCACAGTAACCAGTCACA+AGG - chr1_4:72376983-72377002 Msa0183440:CDS 50.0%
GCCACAGTAACCAGTCACAA+GGG - chr1_4:72376984-72377003 Msa0183440:CDS 50.0%
! ACCCTTGTGACTGGTTACTG+TGG + chr1_4:72376988-72377007 None:intergenic 50.0%
TGAGACAAGACCCTTGTGAC+TGG + chr1_4:72376997-72377016 None:intergenic 50.0%
AAGAGCTCAACCAGTCCACC+AGG + chr1_4:72375247-72375266 None:intergenic 55.0%
CAAGCAGACACAGCAGATGG+AGG - chr1_4:72376701-72376720 Msa0183440:intron 55.0%
! GTGTTTTGTGAGCACAGGCC+AGG - chr1_4:72376906-72376925 Msa0183440:CDS 55.0%
! TTTTGTGAGCACAGGCCAGG+TGG - chr1_4:72376909-72376928 Msa0183440:CDS 55.0%
! GATTCCAGTGCCTGGTGGAC+TGG - chr1_4:72375234-72375253 Msa0183440:CDS 60.0%
TCAACCAGTCCACCAGGCAC+TGG + chr1_4:72375241-72375260 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 72374546 72377257 72374546 ID=Msa0183440;Name=Msa0183440
chr1_4 mRNA 72374546 72377257 72374546 ID=Msa0183440-mRNA-1;Parent=Msa0183440;Name=Msa0183440-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0|0|1|1|1|7|0|528
chr1_4 exon 72377169 72377257 72377169 ID=Msa0183440-mRNA-1:exon:24440;Parent=Msa0183440-mRNA-1
chr1_4 exon 72376882 72377052 72376882 ID=Msa0183440-mRNA-1:exon:24439;Parent=Msa0183440-mRNA-1
chr1_4 exon 72376708 72376786 72376708 ID=Msa0183440-mRNA-1:exon:24438;Parent=Msa0183440-mRNA-1
chr1_4 exon 72376524 72376595 72376524 ID=Msa0183440-mRNA-1:exon:24437;Parent=Msa0183440-mRNA-1
chr1_4 exon 72375081 72375971 72375081 ID=Msa0183440-mRNA-1:exon:24436;Parent=Msa0183440-mRNA-1
chr1_4 exon 72374769 72374945 72374769 ID=Msa0183440-mRNA-1:exon:24435;Parent=Msa0183440-mRNA-1
chr1_4 exon 72374546 72374653 72374546 ID=Msa0183440-mRNA-1:exon:24434;Parent=Msa0183440-mRNA-1
chr1_4 CDS 72377169 72377257 72377169 ID=Msa0183440-mRNA-1:cds;Parent=Msa0183440-mRNA-1
chr1_4 CDS 72376882 72377052 72376882 ID=Msa0183440-mRNA-1:cds;Parent=Msa0183440-mRNA-1
chr1_4 CDS 72376708 72376786 72376708 ID=Msa0183440-mRNA-1:cds;Parent=Msa0183440-mRNA-1
chr1_4 CDS 72376524 72376595 72376524 ID=Msa0183440-mRNA-1:cds;Parent=Msa0183440-mRNA-1
chr1_4 CDS 72375081 72375971 72375081 ID=Msa0183440-mRNA-1:cds;Parent=Msa0183440-mRNA-1
chr1_4 CDS 72374769 72374945 72374769 ID=Msa0183440-mRNA-1:cds;Parent=Msa0183440-mRNA-1
chr1_4 CDS 72374546 72374653 72374546 ID=Msa0183440-mRNA-1:cds;Parent=Msa0183440-mRNA-1
Gene Sequence

>Msa0183440

ATGCAAAACTTTACTGAGAGATTAAGACCTCTTGTTGGTTTGAATGGCTGGGATTACTGTGTCTACTGGAAATTAAGTGAAGATCAAAGGTATCTTGAATGGTTGGGATGTTGTTGTGGTGGGAGTGAAAGCAACCAAAATGGTGGAGAAGAACACCTTTTCCCTGTCTCTTCATGTAGGGATACAATGTTTTCACACCCAAGAACAAACTCTTGTGATCTTCTTTCCCAACTTTCCACTTCCATACCTATAGATTCAGGGATTCATGCACAGGCTCTATTAACTAACCAACCCAATTGGTTGAACTATCCCAATAGCTTGGATCCAAACACTTTACATGAAACAATTAATGGGACCCATGTTTTGATTCCAGTGCCTGGTGGACTGGTTGAGCTCTTTGTAACTAAACAGGTTGGTGAAGATCAAAATGTAATAGATTTTATCACATCTCAATGCATTGTCATGGTGGATCAAGAAGGGATGAACCACTCAATTGGTTTCAATTCAATGAGCAACATGCAATCGAATCCAATTGTGGGTGATGAAAATGATGGAAATAATACAAACAATATTTTCCATTCATCAGAGACATTGAATCTTCCACATGACATATCTGTATTGAACTTCATGCAACAGTTCAATTACAGTTACAATCAACAACAAAACAGAATCAAGAACGACGAAACCTTCTCTGAAGAATACCAAGGTTCTTTTCTTCAGGATCAAGAAAACAACAACACTCTCAAATCACACACAGAAGAGGTGCATGATAACAAAAGCTTGATGACAGATAATAATCAATATATGTTTGTGGACCCATTAGACACAAAAAAGAAGCAAGAACATGTTGTTGGTAGAGCAGATTCTATGTCAGATTGCAGTGATCAGAATGAAGAAGAGGATGATGGAAAATATAGGAGAAGAAGTGGAAAAGGAAACCAATCAAAGAACCTTGTTGCTGAGAGGAAGAGAAGGAAGAAACTGAATGATAGGTTATATAACCTTCGTTCATTGGTTCCAAGAATCTCAAAGCTTGATAGAGCATCTATTCTTGGAGATGCTATTGAATTTGTGAAGGATTTGCAGAAGCAAGTGAAAGAGCTTCAAGATGAACTTGAGGAAAATTCAGACACAGGAACTGAAAGTAACTGTGTATATGTTGATGGTGTTCAAGTTCAACCAAATAATAATAACAATGTTCCAAAAGCAGAACATGGATTTCATGTTGGGAATGGCTATGTTTCAAAACAAAAGCAGGAAGATGTTGGTACTGTTACTGACAAGCAGACACAGCAGATGGAGGCACAAGTGGAAGTGGCTCAGATAGATGGGAATGAGTACTTTGTGAAGGTGTTTTGTGAGCACAGGCCAGGTGGTTTTGTGAAATTGATGGAGGCTTTGAACACTTTAGGCATGGATGTAATGCATGCCACAGTAACCAGTCACAAGGGTCTTGTCTCAAATGTTTTTAAAGTTGAGAATTATGAACAGAAAAAGGAGAGTGAAATAGTTGAGGCTGAAGAAGTTAGAGATTCACTACTAGAGCTGACACGGAACAAATCTTCATGCTGGAACTTGGAATCATGA
Protein sequence

>Msa0183440

MQNFTERLRPLVGLNGWDYCVYWKLSEDQRYLEWLGCCCGGSESNQNGGEEHLFPVSSCRDTMFSHPRTNSCDLLSQLSTSIPIDSGIHAQALLTNQPNWLNYPNSLDPNTLHETINGTHVLIPVPGGLVELFVTKQVGEDQNVIDFITSQCIVMVDQEGMNHSIGFNSMSNMQSNPIVGDENDGNNTNNIFHSSETLNLPHDISVLNFMQQFNYSYNQQQNRIKNDETFSEEYQGSFLQDQENNNTLKSHTEEVHDNKSLMTDNNQYMFVDPLDTKKKQEHVVGRADSMSDCSDQNEEEDDGKYRRRSGKGNQSKNLVAERKRRKKLNDRLYNLRSLVPRISKLDRASILGDAIEFVKDLQKQVKELQDELEENSDTGTESNCVYVDGVQVQPNNNNNVPKAEHGFHVGNGYVSKQKQEDVGTVTDKQTQQMEAQVEVAQIDGNEYFVKVFCEHRPGGFVKLMEALNTLGMDVMHATVTSHKGLVSNVFKVENYEQKKESEIVEAEEVRDSLLELTRNKSSCWNLES*