AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0142300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0142300 MtrunA17_Chr4g0075981 63.415 164 24 2 1 142 1 150 2.53e-64 193
Msa0142300 MtrunA17_Chr4g0075871 71.429 63 18 0 80 142 1 63 1.02e-23 87.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 37 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAACTTCTTTCAGAATT+TGG 0.091898 1_4:+1206179 Msa0142300:five_prime_UTR
TATAATATTGGTGCGATAAA+TGG 0.126613 1_4:+1207734 Msa0142300:three_prime_UTR
CCGATCACTCTTCTAGAATT+TGG 0.195722 1_4:+1206219 Msa0142300:five_prime_UTR
ACAGGATAAATATGAAAATA+TGG 0.284798 1_4:+1207358 Msa0142300:intron
TTATAGGTAACTAGTCATTT+AGG 0.290616 1_4:-1207802 None:intergenic
TGACTAGTTACCTATAAAAT+TGG 0.295752 1_4:+1207808 Msa0142300:three_prime_UTR
TCGTAATAGGACTATCATTA+TGG 0.340697 1_4:+1206927 Msa0142300:intron
AATAGACTACTATATAATAT+TGG 0.346695 1_4:+1207722 Msa0142300:three_prime_UTR
AGCTGGAAGCTTTGCTTGCT+CGG 0.347069 1_4:+1207576 Msa0142300:intron
AAATATCTTGAGGAGTGATT+TGG 0.375811 1_4:-1206151 None:intergenic
TATCATTATTAACAAGCATC+AGG 0.412703 1_4:-1207454 None:intergenic
GATGCTGAAGACATAGTAAC+TGG 0.416061 1_4:+1206994 Msa0142300:CDS
ATAATATTGGTGCGATAAAT+GGG 0.423675 1_4:+1207735 Msa0142300:three_prime_UTR
CAAGTTCTGATAACACGAAT+AGG 0.451357 1_4:+1206953 Msa0142300:CDS
GTACGAGTTATGTAAGTAGA+AGG 0.463059 1_4:-1207866 None:intergenic
CTATTCGTGTTATCAGAACT+TGG 0.465198 1_4:-1206952 None:intergenic
TAAATATGAAAATATGGAAA+AGG 0.473598 1_4:+1207364 Msa0142300:CDS
TTATACTCGCTGCTGCCAGC+TGG 0.485111 1_4:+1206103 Msa0142300:five_prime_UTR
TTACATGCAAGAAGAGGAAA+AGG 0.487878 1_4:+1207646 Msa0142300:CDS
CTGTAACTAACTTCGCCAGC+TGG 0.506799 1_4:-1206118 None:intergenic
CAGAACAGCTGATGAGTTGA+GGG 0.507301 1_4:+1207265 Msa0142300:CDS
TTATTAACTTAGGAAAACCT+TGG 0.510321 1_4:+1207141 Msa0142300:intron
CGGATGAAATGAAATGTGAC+TGG 0.533408 1_4:+1207198 Msa0142300:CDS
TGAAATTTGAAAATATCTTG+AGG 0.539465 1_4:-1206161 None:intergenic
AAGTACTCTTATATATTCGC+AGG 0.548263 1_4:+1207242 Msa0142300:CDS
ACTGGAGGAAAGAGGAATGA+AGG 0.555180 1_4:+1207012 Msa0142300:CDS
GCAGAACAGCTGATGAGTTG+AGG 0.555622 1_4:+1207264 Msa0142300:CDS
ACATAGTAACTGGAGGAAAG+AGG 0.562414 1_4:+1207004 Msa0142300:CDS
AAATGAAATGTGACTGGGCA+AGG 0.566618 1_4:+1207204 Msa0142300:CDS
GCATCAGAAGTAGTCTTCAC+AGG 0.567765 1_4:-1206976 None:intergenic
CCAAATTCTAGAAGAGTGAT+CGG 0.573879 1_4:-1206219 None:intergenic
AGTTAATTACATGCAAGAAG+AGG 0.578388 1_4:+1207640 Msa0142300:CDS
TATTCATCAGATCGCATCCA+AGG 0.582787 1_4:-1207158 None:intergenic
ATGGAAGAAAGCTCAAGAGA+TGG 0.623297 1_4:+1207431 Msa0142300:CDS
GCTGAAGACATAGTAACTGG+AGG 0.677497 1_4:+1206997 Msa0142300:CDS
ATACGTATTGATCAAAGCTG+CGG 0.682966 1_4:+1207178 Msa0142300:CDS
GGATGAAATGAAATGTGACT+GGG 0.699158 1_4:+1207199 Msa0142300:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAATGATAAAAACAAAA+TGG + chr1_4:1207466-1207485 Msa0142300:CDS 10.0%
!! AAAAACATAATACTAAATAT+AGG - chr1_4:1207512-1207531 None:intergenic 10.0%
!! TTGAATAAAATTGAATTAAA+TGG + chr1_4:1207530-1207549 Msa0142300:intron 10.0%
!! TAATAATGATAAAAACAAAA+TGG + chr1_4:1207466-1207485 Msa0142300:CDS 10.0%
!! AAAAACATAATACTAAATAT+AGG - chr1_4:1207512-1207531 None:intergenic 10.0%
!! TTGAATAAAATTGAATTAAA+TGG + chr1_4:1207530-1207549 Msa0142300:intron 10.0%
!! AAAAATCAAGAAAAAACAAT+AGG - chr1_4:1206796-1206815 None:intergenic 15.0%
!! TAAATATGAAAATATGGAAA+AGG + chr1_4:1207364-1207383 Msa0142300:CDS 15.0%
!! AATAGACTACTATATAATAT+TGG + chr1_4:1207722-1207741 Msa0142300:three_prime_UTR 15.0%
!! AAAAATCAAGAAAAAACAAT+AGG - chr1_4:1206796-1206815 None:intergenic 15.0%
!! TAAATATGAAAATATGGAAA+AGG + chr1_4:1207364-1207383 Msa0142300:CDS 15.0%
!! AATAGACTACTATATAATAT+TGG + chr1_4:1207722-1207741 Msa0142300:three_prime_UTR 15.0%
!! TGAAATTTGAAAATATCTTG+AGG - chr1_4:1206164-1206183 None:intergenic 20.0%
!!! GAATATATAAGAGTACTTTT+TGG - chr1_4:1207240-1207259 None:intergenic 20.0%
!! ACAGGATAAATATGAAAATA+TGG + chr1_4:1207358-1207377 Msa0142300:intron 20.0%
!! AACATAATACTAAATATAGG+TGG - chr1_4:1207509-1207528 None:intergenic 20.0%
!!! ACTTGCTATCATTTTTTTTT+GGG - chr1_4:1207834-1207853 None:intergenic 20.0%
!! TGAAATTTGAAAATATCTTG+AGG - chr1_4:1206164-1206183 None:intergenic 20.0%
!!! GAATATATAAGAGTACTTTT+TGG - chr1_4:1207240-1207259 None:intergenic 20.0%
!! ACAGGATAAATATGAAAATA+TGG + chr1_4:1207358-1207377 Msa0142300:intron 20.0%
!! AACATAATACTAAATATAGG+TGG - chr1_4:1207509-1207528 None:intergenic 20.0%
!!! ACTTGCTATCATTTTTTTTT+GGG - chr1_4:1207834-1207853 None:intergenic 20.0%
! TTTCAACTTCTTTCAGAATT+TGG + chr1_4:1206179-1206198 Msa0142300:five_prime_UTR 25.0%
! ACATCAACATCAACAATAAT+AGG - chr1_4:1206464-1206483 None:intergenic 25.0%
!! TGATAATTTTCACTCGTAAT+AGG + chr1_4:1206914-1206933 Msa0142300:intron 25.0%
! CACATACATGTTATTAACTT+AGG + chr1_4:1207131-1207150 Msa0142300:intron 25.0%
! TTATTAACTTAGGAAAACCT+TGG + chr1_4:1207141-1207160 Msa0142300:intron 25.0%
! TATCATTATTAACAAGCATC+AGG - chr1_4:1207457-1207476 None:intergenic 25.0%
! TATAATATTGGTGCGATAAA+TGG + chr1_4:1207734-1207753 Msa0142300:three_prime_UTR 25.0%
! ATAATATTGGTGCGATAAAT+GGG + chr1_4:1207735-1207754 Msa0142300:three_prime_UTR 25.0%
!!! GCATCATCTGTTTTTTTTTT+TGG + chr1_4:1207772-1207791 Msa0142300:three_prime_UTR 25.0%
! TTATAGGTAACTAGTCATTT+AGG - chr1_4:1207805-1207824 None:intergenic 25.0%
!! TGACTAGTTACCTATAAAAT+TGG + chr1_4:1207808-1207827 Msa0142300:three_prime_UTR 25.0%
!!! TTTTTTTGGGCCAATTTTAT+AGG - chr1_4:1207821-1207840 None:intergenic 25.0%
!!! GACTTGCTATCATTTTTTTT+TGG - chr1_4:1207835-1207854 None:intergenic 25.0%
! TTTCAACTTCTTTCAGAATT+TGG + chr1_4:1206179-1206198 Msa0142300:five_prime_UTR 25.0%
! ACATCAACATCAACAATAAT+AGG - chr1_4:1206464-1206483 None:intergenic 25.0%
!! TGATAATTTTCACTCGTAAT+AGG + chr1_4:1206914-1206933 Msa0142300:intron 25.0%
! CACATACATGTTATTAACTT+AGG + chr1_4:1207131-1207150 Msa0142300:intron 25.0%
! TTATTAACTTAGGAAAACCT+TGG + chr1_4:1207141-1207160 Msa0142300:intron 25.0%
! TATCATTATTAACAAGCATC+AGG - chr1_4:1207457-1207476 None:intergenic 25.0%
! TATAATATTGGTGCGATAAA+TGG + chr1_4:1207734-1207753 Msa0142300:three_prime_UTR 25.0%
! ATAATATTGGTGCGATAAAT+GGG + chr1_4:1207735-1207754 Msa0142300:three_prime_UTR 25.0%
!!! GCATCATCTGTTTTTTTTTT+TGG + chr1_4:1207772-1207791 Msa0142300:three_prime_UTR 25.0%
! TTATAGGTAACTAGTCATTT+AGG - chr1_4:1207805-1207824 None:intergenic 25.0%
!! TGACTAGTTACCTATAAAAT+TGG + chr1_4:1207808-1207827 Msa0142300:three_prime_UTR 25.0%
!!! TTTTTTTGGGCCAATTTTAT+AGG - chr1_4:1207821-1207840 None:intergenic 25.0%
!!! GACTTGCTATCATTTTTTTT+TGG - chr1_4:1207835-1207854 None:intergenic 25.0%
AAATATCTTGAGGAGTGATT+TGG - chr1_4:1206154-1206173 None:intergenic 30.0%
!! TGTTGATAATGATGAATCTC+CGG + chr1_4:1206481-1206500 Msa0142300:intron 30.0%
CAAAACAAAACAAACCAAAC+CGG - chr1_4:1206503-1206522 None:intergenic 30.0%
!!! TGATTTTTTTTCACTCACGA+TGG + chr1_4:1206635-1206654 Msa0142300:intron 30.0%
AACAACATCAGTAGGATTAT+TGG - chr1_4:1206821-1206840 None:intergenic 30.0%
TATAGATGAACAACATCAGT+AGG - chr1_4:1206829-1206848 None:intergenic 30.0%
TGTGATTTCAGTCTTAGTTT+AGG + chr1_4:1206880-1206899 Msa0142300:intron 30.0%
TCGTAATAGGACTATCATTA+TGG + chr1_4:1206927-1206946 Msa0142300:intron 30.0%
! ATATGATGCTTTGTTGTATG+TGG + chr1_4:1207056-1207075 Msa0142300:intron 30.0%
AAGTACTCTTATATATTCGC+AGG + chr1_4:1207242-1207261 Msa0142300:CDS 30.0%
ATACAATACGTACAAAGACT+AGG - chr1_4:1207316-1207335 None:intergenic 30.0%
ATTGTCTTCAAATTGCATAC+AGG + chr1_4:1207340-1207359 Msa0142300:intron 30.0%
TGCAAAAAAGAAACAACAGA+TGG + chr1_4:1207412-1207431 Msa0142300:CDS 30.0%
! TTTTTCTTTTTACTTCCAGC+TGG + chr1_4:1207559-1207578 Msa0142300:intron 30.0%
AGTTAATTACATGCAAGAAG+AGG + chr1_4:1207640-1207659 Msa0142300:CDS 30.0%
AAATATCTTGAGGAGTGATT+TGG - chr1_4:1206154-1206173 None:intergenic 30.0%
!! TGTTGATAATGATGAATCTC+CGG + chr1_4:1206481-1206500 Msa0142300:intron 30.0%
CAAAACAAAACAAACCAAAC+CGG - chr1_4:1206503-1206522 None:intergenic 30.0%
!!! TGATTTTTTTTCACTCACGA+TGG + chr1_4:1206635-1206654 Msa0142300:intron 30.0%
AACAACATCAGTAGGATTAT+TGG - chr1_4:1206821-1206840 None:intergenic 30.0%
TATAGATGAACAACATCAGT+AGG - chr1_4:1206829-1206848 None:intergenic 30.0%
TGTGATTTCAGTCTTAGTTT+AGG + chr1_4:1206880-1206899 Msa0142300:intron 30.0%
TCGTAATAGGACTATCATTA+TGG + chr1_4:1206927-1206946 Msa0142300:intron 30.0%
! ATATGATGCTTTGTTGTATG+TGG + chr1_4:1207056-1207075 Msa0142300:intron 30.0%
AAGTACTCTTATATATTCGC+AGG + chr1_4:1207242-1207261 Msa0142300:CDS 30.0%
ATACAATACGTACAAAGACT+AGG - chr1_4:1207316-1207335 None:intergenic 30.0%
ATTGTCTTCAAATTGCATAC+AGG + chr1_4:1207340-1207359 Msa0142300:intron 30.0%
TGCAAAAAAGAAACAACAGA+TGG + chr1_4:1207412-1207431 Msa0142300:CDS 30.0%
! TTTTTCTTTTTACTTCCAGC+TGG + chr1_4:1207559-1207578 Msa0142300:intron 30.0%
AGTTAATTACATGCAAGAAG+AGG + chr1_4:1207640-1207659 Msa0142300:CDS 30.0%
! CCAAATTCTAGAAGAGTGAT+CGG - chr1_4:1206222-1206241 None:intergenic 35.0%
ATCATAGAAAGAGGTTCGAA+CGG - chr1_4:1206349-1206368 None:intergenic 35.0%
AGGATTGGAATCATAGAAAG+AGG - chr1_4:1206358-1206377 None:intergenic 35.0%
ATGGAACACAAAATCAGGAT+TGG - chr1_4:1206373-1206392 None:intergenic 35.0%
GATAGATGGAACACAAAATC+AGG - chr1_4:1206378-1206397 None:intergenic 35.0%
ATAATGATGAATCTCCGGTT+TGG + chr1_4:1206486-1206505 Msa0142300:intron 35.0%
CTATTCGTGTTATCAGAACT+TGG - chr1_4:1206955-1206974 None:intergenic 35.0%
! CAAGTTCTGATAACACGAAT+AGG + chr1_4:1206953-1206972 Msa0142300:CDS 35.0%
GTATGTGGTATGCTAATCAA+TGG + chr1_4:1207071-1207090 Msa0142300:intron 35.0%
! ATACGTATTGATCAAAGCTG+CGG + chr1_4:1207178-1207197 Msa0142300:CDS 35.0%
GGATGAAATGAAATGTGACT+GGG + chr1_4:1207199-1207218 Msa0142300:CDS 35.0%
TTACATGCAAGAAGAGGAAA+AGG + chr1_4:1207646-1207665 Msa0142300:CDS 35.0%
! CCAAATTCTAGAAGAGTGAT+CGG - chr1_4:1206222-1206241 None:intergenic 35.0%
ATCATAGAAAGAGGTTCGAA+CGG - chr1_4:1206349-1206368 None:intergenic 35.0%
AGGATTGGAATCATAGAAAG+AGG - chr1_4:1206358-1206377 None:intergenic 35.0%
ATGGAACACAAAATCAGGAT+TGG - chr1_4:1206373-1206392 None:intergenic 35.0%
GATAGATGGAACACAAAATC+AGG - chr1_4:1206378-1206397 None:intergenic 35.0%
ATAATGATGAATCTCCGGTT+TGG + chr1_4:1206486-1206505 Msa0142300:intron 35.0%
CTATTCGTGTTATCAGAACT+TGG - chr1_4:1206955-1206974 None:intergenic 35.0%
! CAAGTTCTGATAACACGAAT+AGG + chr1_4:1206953-1206972 Msa0142300:CDS 35.0%
GTATGTGGTATGCTAATCAA+TGG + chr1_4:1207071-1207090 Msa0142300:intron 35.0%
! ATACGTATTGATCAAAGCTG+CGG + chr1_4:1207178-1207197 Msa0142300:CDS 35.0%
GGATGAAATGAAATGTGACT+GGG + chr1_4:1207199-1207218 Msa0142300:CDS 35.0%
TTACATGCAAGAAGAGGAAA+AGG + chr1_4:1207646-1207665 Msa0142300:CDS 35.0%
!! CCGATCACTCTTCTAGAATT+TGG + chr1_4:1206219-1206238 Msa0142300:five_prime_UTR 40.0%
CATCGATCAACAACAGCATT+AGG - chr1_4:1206321-1206340 None:intergenic 40.0%
GATGCTGAAGACATAGTAAC+TGG + chr1_4:1206994-1207013 Msa0142300:CDS 40.0%
ACATAGTAACTGGAGGAAAG+AGG + chr1_4:1207004-1207023 Msa0142300:CDS 40.0%
TATTCATCAGATCGCATCCA+AGG - chr1_4:1207161-1207180 None:intergenic 40.0%
CGGATGAAATGAAATGTGAC+TGG + chr1_4:1207198-1207217 Msa0142300:CDS 40.0%
AAATGAAATGTGACTGGGCA+AGG + chr1_4:1207204-1207223 Msa0142300:CDS 40.0%
ATGGAAGAAAGCTCAAGAGA+TGG + chr1_4:1207431-1207450 Msa0142300:CDS 40.0%
!! CCGATCACTCTTCTAGAATT+TGG + chr1_4:1206219-1206238 Msa0142300:five_prime_UTR 40.0%
CATCGATCAACAACAGCATT+AGG - chr1_4:1206321-1206340 None:intergenic 40.0%
GATGCTGAAGACATAGTAAC+TGG + chr1_4:1206994-1207013 Msa0142300:CDS 40.0%
ACATAGTAACTGGAGGAAAG+AGG + chr1_4:1207004-1207023 Msa0142300:CDS 40.0%
TATTCATCAGATCGCATCCA+AGG - chr1_4:1207161-1207180 None:intergenic 40.0%
CGGATGAAATGAAATGTGAC+TGG + chr1_4:1207198-1207217 Msa0142300:CDS 40.0%
AAATGAAATGTGACTGGGCA+AGG + chr1_4:1207204-1207223 Msa0142300:CDS 40.0%
ATGGAAGAAAGCTCAAGAGA+TGG + chr1_4:1207431-1207450 Msa0142300:CDS 40.0%
! GTTCCATCTATCGCTGTTTC+AGG + chr1_4:1206386-1206405 Msa0142300:intron 45.0%
GCATCAGAAGTAGTCTTCAC+AGG - chr1_4:1206979-1206998 None:intergenic 45.0%
GCTGAAGACATAGTAACTGG+AGG + chr1_4:1206997-1207016 Msa0142300:CDS 45.0%
ACTGGAGGAAAGAGGAATGA+AGG + chr1_4:1207012-1207031 Msa0142300:CDS 45.0%
! CAGAACAGCTGATGAGTTGA+GGG + chr1_4:1207265-1207284 Msa0142300:CDS 45.0%
! GTTCCATCTATCGCTGTTTC+AGG + chr1_4:1206386-1206405 Msa0142300:intron 45.0%
GCATCAGAAGTAGTCTTCAC+AGG - chr1_4:1206979-1206998 None:intergenic 45.0%
GCTGAAGACATAGTAACTGG+AGG + chr1_4:1206997-1207016 Msa0142300:CDS 45.0%
ACTGGAGGAAAGAGGAATGA+AGG + chr1_4:1207012-1207031 Msa0142300:CDS 45.0%
! CAGAACAGCTGATGAGTTGA+GGG + chr1_4:1207265-1207284 Msa0142300:CDS 45.0%
!!! ATAGATTTTTTTTTTTTTTT+TGG + chr1_4:1207673-1207692 Msa0142300:exon 5.0%
!!! TAGATTTTTTTTTTTTTTTT+GGG + chr1_4:1207674-1207693 Msa0142300:exon 5.0%
!!! ATAGATTTTTTTTTTTTTTT+TGG + chr1_4:1207673-1207692 Msa0142300:exon 5.0%
!!! TAGATTTTTTTTTTTTTTTT+GGG + chr1_4:1207674-1207693 Msa0142300:exon 5.0%
CTGTAACTAACTTCGCCAGC+TGG - chr1_4:1206121-1206140 None:intergenic 50.0%
GAGCCTGAAACAGCGATAGA+TGG - chr1_4:1206392-1206411 None:intergenic 50.0%
GCAGAACAGCTGATGAGTTG+AGG + chr1_4:1207264-1207283 Msa0142300:CDS 50.0%
AGCTGGAAGCTTTGCTTGCT+CGG + chr1_4:1207576-1207595 Msa0142300:intron 50.0%
CTGTAACTAACTTCGCCAGC+TGG - chr1_4:1206121-1206140 None:intergenic 50.0%
GAGCCTGAAACAGCGATAGA+TGG - chr1_4:1206392-1206411 None:intergenic 50.0%
GCAGAACAGCTGATGAGTTG+AGG + chr1_4:1207264-1207283 Msa0142300:CDS 50.0%
AGCTGGAAGCTTTGCTTGCT+CGG + chr1_4:1207576-1207595 Msa0142300:intron 50.0%
TTATACTCGCTGCTGCCAGC+TGG + chr1_4:1206103-1206122 Msa0142300:five_prime_UTR 55.0%
GAGCAAGCAAAGCTTCCAGC+TGG - chr1_4:1207577-1207596 None:intergenic 55.0%
TTATACTCGCTGCTGCCAGC+TGG + chr1_4:1206103-1206122 Msa0142300:five_prime_UTR 55.0%
GAGCAAGCAAAGCTTCCAGC+TGG - chr1_4:1207577-1207596 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 1206103 1207884 1206103 ID=Msa0142300;Name=Msa0142300
chr1_4 mRNA 1206103 1207884 1206103 ID=Msa0142300-mRNA-1;Parent=Msa0142300;Name=Msa0142300-mRNA-1;_AED=0.09;_eAED=0.09;_QI=157|1|1|1|0.75|0.6|5|208|148
chr1_4 exon 1206103 1206249 1206103 ID=Msa0142300-mRNA-1:exon:372;Parent=Msa0142300-mRNA-1
chr1_4 exon 1206936 1207033 1206936 ID=Msa0142300-mRNA-1:exon:373;Parent=Msa0142300-mRNA-1
chr1_4 exon 1207153 1207286 1207153 ID=Msa0142300-mRNA-1:exon:374;Parent=Msa0142300-mRNA-1
chr1_4 exon 1207362 1207487 1207362 ID=Msa0142300-mRNA-1:exon:375;Parent=Msa0142300-mRNA-1
chr1_4 exon 1207578 1207884 1207578 ID=Msa0142300-mRNA-1:exon:376;Parent=Msa0142300-mRNA-1
chr1_4 five_prime_UTR 1206103 1206249 1206103 ID=Msa0142300-mRNA-1:five_prime_utr;Parent=Msa0142300-mRNA-1
chr1_4 five_prime_UTR 1206936 1206945 1206936 ID=Msa0142300-mRNA-1:five_prime_utr;Parent=Msa0142300-mRNA-1
chr1_4 CDS 1206946 1207033 1206946 ID=Msa0142300-mRNA-1:cds;Parent=Msa0142300-mRNA-1
chr1_4 CDS 1207153 1207286 1207153 ID=Msa0142300-mRNA-1:cds;Parent=Msa0142300-mRNA-1
chr1_4 CDS 1207362 1207487 1207362 ID=Msa0142300-mRNA-1:cds;Parent=Msa0142300-mRNA-1
chr1_4 CDS 1207578 1207676 1207578 ID=Msa0142300-mRNA-1:cds;Parent=Msa0142300-mRNA-1
chr1_4 three_prime_UTR 1207677 1207884 1207677 ID=Msa0142300-mRNA-1:three_prime_utr;Parent=Msa0142300-mRNA-1
Gene Sequence

>Msa0142300

ATGGAGCCAAGTTCTGATAACACGAATAGGCCTGTGAAGACTACTTCTGATGCTGAAGACATAGTAACTGGAGGAAAGAGGAATGAAGGAAAACCTTGGATGCGATCTGATGAATACGTATTGATCAAAGCTGCGGATGAAATGAAATGTGACTGGGCAAGGATATTGACATCCAAAAAGTACTCTTATATATTCGCAGGCAGAACAGCTGATGAGTTGAGGGATAAATATGAAAATATGGAAAAGGATAAATATGAACAAATGCTACAAGATGCAAAAAAGAAACAACAGATGGAAGAAAGCTCAAGAGATGGCCTGATGCTTGTTAATAATGATAAAAACAAAATGCTGGAAGCTTTGCTTGCTCGGAATGAGTTGCTGAGAAAGACTTCAGAGAAAATGAACATTGTAGTTAATTACATGCAAGAAGAGGAAAAGGATGAATAG
Protein sequence

>Msa0142300

MEPSSDNTNRPVKTTSDAEDIVTGGKRNEGKPWMRSDEYVLIKAADEMKCDWARILTSKKYSYIFAGRTADELRDKYENMEKDKYEQMLQDAKKKQQMEESSRDGLMLVNNDKNKMLEALLARNELLRKTSEKMNIVVNYMQEEEKDE*