AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0692670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0692670 MtrunA17_Chr4g0075981 64.024 164 23 2 1 142 1 150 3.76e-65 195
Msa0692670 MtrunA17_Chr4g0075871 73.016 63 17 0 80 142 1 63 1.67e-24 89.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 38 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAACTTCTTTCAGAATT+TGG 0.091898 4_4:+61309423 Msa0692670:five_prime_UTR
TATAATATTGGTGCGATAAA+TGG 0.126613 4_4:+61310980 Msa0692670:three_prime_UTR
CCGATCACTCTTCTAGAATT+TGG 0.195722 4_4:+61309463 Msa0692670:five_prime_UTR
GAGAAAGACTTCAGAGAAAA+TGG 0.256513 4_4:+61310855 Msa0692670:CDS
ACAGGATAAATATGAAAATA+TGG 0.284798 4_4:+61310603 Msa0692670:intron
TTATAGGTAACTAGTCATTT+AGG 0.290616 4_4:-61311047 None:intergenic
TGACTAGTTACCTATAAAAT+TGG 0.295752 4_4:+61311053 Msa0692670:three_prime_UTR
TCGTAATAGGACTATCATTA+TGG 0.340697 4_4:+61310171 Msa0692670:intron
AATAGACTACTATATAATAT+TGG 0.346695 4_4:+61310968 Msa0692670:three_prime_UTR
AGCTGGAAGCTTTGCTTGCT+CGG 0.347069 4_4:+61310821 Msa0692670:intron
AAATATCTTGAGGAGTGATT+TGG 0.375811 4_4:-61309395 None:intergenic
TATCATTATTAACAAGCATC+AGG 0.412703 4_4:-61310699 None:intergenic
GATGCTGAAGACATAGTAAC+TGG 0.416061 4_4:+61310238 Msa0692670:CDS
ATAATATTGGTGCGATAAAT+GGG 0.423675 4_4:+61310981 Msa0692670:three_prime_UTR
CAAGTTCTGATAACACGAAT+AGG 0.451357 4_4:+61310197 Msa0692670:CDS
GTACGAGTTATGTAAGTAGA+AGG 0.463059 4_4:-61311111 None:intergenic
CTATTCGTGTTATCAGAACT+TGG 0.465198 4_4:-61310196 None:intergenic
TAAATATGAAAATATGGAAA+AGG 0.473598 4_4:+61310609 Msa0692670:CDS
TTATACTCGCTGCTGCCAGC+TGG 0.485111 4_4:+61309347 Msa0692670:five_prime_UTR
TTACATGCAAGAAGAGGAAA+AGG 0.487878 4_4:+61310891 Msa0692670:CDS
CTGTAACTAACTTCGCCAGC+TGG 0.506799 4_4:-61309362 None:intergenic
CAGAACAGCTGATGAGTTGA+GGG 0.507301 4_4:+61310509 Msa0692670:CDS
TTATTAACTTAGGAAAACCT+TGG 0.510321 4_4:+61310385 Msa0692670:intron
CGGATGAAATGAAATGTGAC+TGG 0.533408 4_4:+61310442 Msa0692670:CDS
TGAAATTTGAAAATATCTTG+AGG 0.539465 4_4:-61309405 None:intergenic
AAGTACTCTTATATATTCGC+AGG 0.548263 4_4:+61310486 Msa0692670:CDS
ACTGGAGGAAAGAGGAATGA+AGG 0.555180 4_4:+61310256 Msa0692670:CDS
GCAGAACAGCTGATGAGTTG+AGG 0.555622 4_4:+61310508 Msa0692670:CDS
ACATAGTAACTGGAGGAAAG+AGG 0.562414 4_4:+61310248 Msa0692670:CDS
AAATGAAATGTGACTGGGCA+AGG 0.566618 4_4:+61310448 Msa0692670:CDS
GCATCAGAAGTAGTCTTCAC+AGG 0.567765 4_4:-61310220 None:intergenic
CCAAATTCTAGAAGAGTGAT+CGG 0.573879 4_4:-61309463 None:intergenic
AGTTAATTACATGCAAGAAG+AGG 0.578388 4_4:+61310885 Msa0692670:CDS
TATTCATCAGATCGCATCCA+AGG 0.582787 4_4:-61310402 None:intergenic
ATGGAAGAAAGCTCAAGAGA+TGG 0.623297 4_4:+61310676 Msa0692670:CDS
GCTGAAGACATAGTAACTGG+AGG 0.677497 4_4:+61310241 Msa0692670:CDS
ATACGTATTGATCAAAGCTG+CGG 0.682966 4_4:+61310422 Msa0692670:CDS
GGATGAAATGAAATGTGACT+GGG 0.699158 4_4:+61310443 Msa0692670:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAATGATAAAAACAAAA+TGG + chr4_4:61310711-61310730 Msa0692670:CDS 10.0%
!! AAAAACATAATACTAAATAT+AGG - chr4_4:61310757-61310776 None:intergenic 10.0%
!! TTGAATAAAATTGAATTAAA+TGG + chr4_4:61310775-61310794 Msa0692670:intron 10.0%
!! TAATAATGATAAAAACAAAA+TGG + chr4_4:61310711-61310730 Msa0692670:CDS 10.0%
!! AAAAACATAATACTAAATAT+AGG - chr4_4:61310757-61310776 None:intergenic 10.0%
!! TTGAATAAAATTGAATTAAA+TGG + chr4_4:61310775-61310794 Msa0692670:intron 10.0%
!! AAAAATCAAGAAAAAACAAT+AGG - chr4_4:61310040-61310059 None:intergenic 15.0%
!! TAAATATGAAAATATGGAAA+AGG + chr4_4:61310609-61310628 Msa0692670:CDS 15.0%
!! AATAGACTACTATATAATAT+TGG + chr4_4:61310968-61310987 Msa0692670:three_prime_UTR 15.0%
!! AAAAATCAAGAAAAAACAAT+AGG - chr4_4:61310040-61310059 None:intergenic 15.0%
!! TAAATATGAAAATATGGAAA+AGG + chr4_4:61310609-61310628 Msa0692670:CDS 15.0%
!! AATAGACTACTATATAATAT+TGG + chr4_4:61310968-61310987 Msa0692670:three_prime_UTR 15.0%
!! TGAAATTTGAAAATATCTTG+AGG - chr4_4:61309408-61309427 None:intergenic 20.0%
!!! GAATATATAAGAGTACTTTT+TGG - chr4_4:61310484-61310503 None:intergenic 20.0%
!! ACAGGATAAATATGAAAATA+TGG + chr4_4:61310603-61310622 Msa0692670:intron 20.0%
!! AACATAATACTAAATATAGG+TGG - chr4_4:61310754-61310773 None:intergenic 20.0%
!!! ACTTGCTATCATTTTTTTTT+GGG - chr4_4:61311079-61311098 None:intergenic 20.0%
!! TGAAATTTGAAAATATCTTG+AGG - chr4_4:61309408-61309427 None:intergenic 20.0%
!!! GAATATATAAGAGTACTTTT+TGG - chr4_4:61310484-61310503 None:intergenic 20.0%
!! ACAGGATAAATATGAAAATA+TGG + chr4_4:61310603-61310622 Msa0692670:intron 20.0%
!! AACATAATACTAAATATAGG+TGG - chr4_4:61310754-61310773 None:intergenic 20.0%
!!! ACTTGCTATCATTTTTTTTT+GGG - chr4_4:61311079-61311098 None:intergenic 20.0%
! TTTCAACTTCTTTCAGAATT+TGG + chr4_4:61309423-61309442 Msa0692670:five_prime_UTR 25.0%
! ACATCAACATCAACAATAAT+AGG - chr4_4:61309708-61309727 None:intergenic 25.0%
!! TGATAATTTTCACTCGTAAT+AGG + chr4_4:61310158-61310177 Msa0692670:intron 25.0%
! CACATACATGTTATTAACTT+AGG + chr4_4:61310375-61310394 Msa0692670:intron 25.0%
! TTATTAACTTAGGAAAACCT+TGG + chr4_4:61310385-61310404 Msa0692670:intron 25.0%
! TATCATTATTAACAAGCATC+AGG - chr4_4:61310702-61310721 None:intergenic 25.0%
! TATAATATTGGTGCGATAAA+TGG + chr4_4:61310980-61310999 Msa0692670:three_prime_UTR 25.0%
! ATAATATTGGTGCGATAAAT+GGG + chr4_4:61310981-61311000 Msa0692670:three_prime_UTR 25.0%
!!! TGCATCATCTGTTTTTTTTT+TGG + chr4_4:61311017-61311036 Msa0692670:three_prime_UTR 25.0%
! TTATAGGTAACTAGTCATTT+AGG - chr4_4:61311050-61311069 None:intergenic 25.0%
!! TGACTAGTTACCTATAAAAT+TGG + chr4_4:61311053-61311072 Msa0692670:three_prime_UTR 25.0%
!!! TTTTTTTGGGCCAATTTTAT+AGG - chr4_4:61311066-61311085 None:intergenic 25.0%
!!! GACTTGCTATCATTTTTTTT+TGG - chr4_4:61311080-61311099 None:intergenic 25.0%
! TTTCAACTTCTTTCAGAATT+TGG + chr4_4:61309423-61309442 Msa0692670:five_prime_UTR 25.0%
! ACATCAACATCAACAATAAT+AGG - chr4_4:61309708-61309727 None:intergenic 25.0%
!! TGATAATTTTCACTCGTAAT+AGG + chr4_4:61310158-61310177 Msa0692670:intron 25.0%
! CACATACATGTTATTAACTT+AGG + chr4_4:61310375-61310394 Msa0692670:intron 25.0%
! TTATTAACTTAGGAAAACCT+TGG + chr4_4:61310385-61310404 Msa0692670:intron 25.0%
! TATCATTATTAACAAGCATC+AGG - chr4_4:61310702-61310721 None:intergenic 25.0%
! TATAATATTGGTGCGATAAA+TGG + chr4_4:61310980-61310999 Msa0692670:three_prime_UTR 25.0%
! ATAATATTGGTGCGATAAAT+GGG + chr4_4:61310981-61311000 Msa0692670:three_prime_UTR 25.0%
!!! TGCATCATCTGTTTTTTTTT+TGG + chr4_4:61311017-61311036 Msa0692670:three_prime_UTR 25.0%
! TTATAGGTAACTAGTCATTT+AGG - chr4_4:61311050-61311069 None:intergenic 25.0%
!! TGACTAGTTACCTATAAAAT+TGG + chr4_4:61311053-61311072 Msa0692670:three_prime_UTR 25.0%
!!! TTTTTTTGGGCCAATTTTAT+AGG - chr4_4:61311066-61311085 None:intergenic 25.0%
!!! GACTTGCTATCATTTTTTTT+TGG - chr4_4:61311080-61311099 None:intergenic 25.0%
AAATATCTTGAGGAGTGATT+TGG - chr4_4:61309398-61309417 None:intergenic 30.0%
!! TGTTGATAATGATGAATCTC+CGG + chr4_4:61309725-61309744 Msa0692670:intron 30.0%
CAAAACAAAACAAACCAAAC+CGG - chr4_4:61309747-61309766 None:intergenic 30.0%
!!! TGATTTTTTTTCACTCACGA+TGG + chr4_4:61309879-61309898 Msa0692670:intron 30.0%
AACAACATCAGTAGGATTAT+TGG - chr4_4:61310065-61310084 None:intergenic 30.0%
TATAGATGAACAACATCAGT+AGG - chr4_4:61310073-61310092 None:intergenic 30.0%
TGTGATTTCAGTCTTAGTTT+AGG + chr4_4:61310124-61310143 Msa0692670:intron 30.0%
TCGTAATAGGACTATCATTA+TGG + chr4_4:61310171-61310190 Msa0692670:intron 30.0%
! ATATGATGCTTTGTTGTATG+TGG + chr4_4:61310300-61310319 Msa0692670:intron 30.0%
AAGTACTCTTATATATTCGC+AGG + chr4_4:61310486-61310505 Msa0692670:CDS 30.0%
TACAATAACGTACAAAGACT+AGG - chr4_4:61310560-61310579 None:intergenic 30.0%
ATTGTCTTCAAATTGCATAC+AGG + chr4_4:61310585-61310604 Msa0692670:intron 30.0%
TGCAAAAAAGAAACAACAGA+TGG + chr4_4:61310657-61310676 Msa0692670:CDS 30.0%
! TTTTTCTTTTTACTTCCAGC+TGG + chr4_4:61310804-61310823 Msa0692670:intron 30.0%
AGTTAATTACATGCAAGAAG+AGG + chr4_4:61310885-61310904 Msa0692670:CDS 30.0%
AAATATCTTGAGGAGTGATT+TGG - chr4_4:61309398-61309417 None:intergenic 30.0%
!! TGTTGATAATGATGAATCTC+CGG + chr4_4:61309725-61309744 Msa0692670:intron 30.0%
CAAAACAAAACAAACCAAAC+CGG - chr4_4:61309747-61309766 None:intergenic 30.0%
!!! TGATTTTTTTTCACTCACGA+TGG + chr4_4:61309879-61309898 Msa0692670:intron 30.0%
AACAACATCAGTAGGATTAT+TGG - chr4_4:61310065-61310084 None:intergenic 30.0%
TATAGATGAACAACATCAGT+AGG - chr4_4:61310073-61310092 None:intergenic 30.0%
TGTGATTTCAGTCTTAGTTT+AGG + chr4_4:61310124-61310143 Msa0692670:intron 30.0%
TCGTAATAGGACTATCATTA+TGG + chr4_4:61310171-61310190 Msa0692670:intron 30.0%
! ATATGATGCTTTGTTGTATG+TGG + chr4_4:61310300-61310319 Msa0692670:intron 30.0%
AAGTACTCTTATATATTCGC+AGG + chr4_4:61310486-61310505 Msa0692670:CDS 30.0%
TACAATAACGTACAAAGACT+AGG - chr4_4:61310560-61310579 None:intergenic 30.0%
ATTGTCTTCAAATTGCATAC+AGG + chr4_4:61310585-61310604 Msa0692670:intron 30.0%
TGCAAAAAAGAAACAACAGA+TGG + chr4_4:61310657-61310676 Msa0692670:CDS 30.0%
! TTTTTCTTTTTACTTCCAGC+TGG + chr4_4:61310804-61310823 Msa0692670:intron 30.0%
AGTTAATTACATGCAAGAAG+AGG + chr4_4:61310885-61310904 Msa0692670:CDS 30.0%
! CCAAATTCTAGAAGAGTGAT+CGG - chr4_4:61309466-61309485 None:intergenic 35.0%
ATCATAGAAAGAGGTTCGAA+CGG - chr4_4:61309593-61309612 None:intergenic 35.0%
AGGATTGGAATCATAGAAAG+AGG - chr4_4:61309602-61309621 None:intergenic 35.0%
ATGGAACACAAAATCAGGAT+TGG - chr4_4:61309617-61309636 None:intergenic 35.0%
GATAGATGGAACACAAAATC+AGG - chr4_4:61309622-61309641 None:intergenic 35.0%
ATAATGATGAATCTCCGGTT+TGG + chr4_4:61309730-61309749 Msa0692670:intron 35.0%
CTATTCGTGTTATCAGAACT+TGG - chr4_4:61310199-61310218 None:intergenic 35.0%
! CAAGTTCTGATAACACGAAT+AGG + chr4_4:61310197-61310216 Msa0692670:CDS 35.0%
GTATGTGGTATGCTAATCAA+TGG + chr4_4:61310315-61310334 Msa0692670:intron 35.0%
! ATACGTATTGATCAAAGCTG+CGG + chr4_4:61310422-61310441 Msa0692670:CDS 35.0%
GGATGAAATGAAATGTGACT+GGG + chr4_4:61310443-61310462 Msa0692670:CDS 35.0%
GAGAAAGACTTCAGAGAAAA+TGG + chr4_4:61310855-61310874 Msa0692670:CDS 35.0%
TTACATGCAAGAAGAGGAAA+AGG + chr4_4:61310891-61310910 Msa0692670:CDS 35.0%
! CCAAATTCTAGAAGAGTGAT+CGG - chr4_4:61309466-61309485 None:intergenic 35.0%
ATCATAGAAAGAGGTTCGAA+CGG - chr4_4:61309593-61309612 None:intergenic 35.0%
AGGATTGGAATCATAGAAAG+AGG - chr4_4:61309602-61309621 None:intergenic 35.0%
ATGGAACACAAAATCAGGAT+TGG - chr4_4:61309617-61309636 None:intergenic 35.0%
GATAGATGGAACACAAAATC+AGG - chr4_4:61309622-61309641 None:intergenic 35.0%
ATAATGATGAATCTCCGGTT+TGG + chr4_4:61309730-61309749 Msa0692670:intron 35.0%
CTATTCGTGTTATCAGAACT+TGG - chr4_4:61310199-61310218 None:intergenic 35.0%
! CAAGTTCTGATAACACGAAT+AGG + chr4_4:61310197-61310216 Msa0692670:CDS 35.0%
GTATGTGGTATGCTAATCAA+TGG + chr4_4:61310315-61310334 Msa0692670:intron 35.0%
! ATACGTATTGATCAAAGCTG+CGG + chr4_4:61310422-61310441 Msa0692670:CDS 35.0%
GGATGAAATGAAATGTGACT+GGG + chr4_4:61310443-61310462 Msa0692670:CDS 35.0%
GAGAAAGACTTCAGAGAAAA+TGG + chr4_4:61310855-61310874 Msa0692670:CDS 35.0%
TTACATGCAAGAAGAGGAAA+AGG + chr4_4:61310891-61310910 Msa0692670:CDS 35.0%
!! CCGATCACTCTTCTAGAATT+TGG + chr4_4:61309463-61309482 Msa0692670:five_prime_UTR 40.0%
CATCGATCAACAACAGCATT+AGG - chr4_4:61309565-61309584 None:intergenic 40.0%
GATGCTGAAGACATAGTAAC+TGG + chr4_4:61310238-61310257 Msa0692670:CDS 40.0%
ACATAGTAACTGGAGGAAAG+AGG + chr4_4:61310248-61310267 Msa0692670:CDS 40.0%
TATTCATCAGATCGCATCCA+AGG - chr4_4:61310405-61310424 None:intergenic 40.0%
CGGATGAAATGAAATGTGAC+TGG + chr4_4:61310442-61310461 Msa0692670:CDS 40.0%
AAATGAAATGTGACTGGGCA+AGG + chr4_4:61310448-61310467 Msa0692670:CDS 40.0%
ATGGAAGAAAGCTCAAGAGA+TGG + chr4_4:61310676-61310695 Msa0692670:CDS 40.0%
!! CCGATCACTCTTCTAGAATT+TGG + chr4_4:61309463-61309482 Msa0692670:five_prime_UTR 40.0%
CATCGATCAACAACAGCATT+AGG - chr4_4:61309565-61309584 None:intergenic 40.0%
GATGCTGAAGACATAGTAAC+TGG + chr4_4:61310238-61310257 Msa0692670:CDS 40.0%
ACATAGTAACTGGAGGAAAG+AGG + chr4_4:61310248-61310267 Msa0692670:CDS 40.0%
TATTCATCAGATCGCATCCA+AGG - chr4_4:61310405-61310424 None:intergenic 40.0%
CGGATGAAATGAAATGTGAC+TGG + chr4_4:61310442-61310461 Msa0692670:CDS 40.0%
AAATGAAATGTGACTGGGCA+AGG + chr4_4:61310448-61310467 Msa0692670:CDS 40.0%
ATGGAAGAAAGCTCAAGAGA+TGG + chr4_4:61310676-61310695 Msa0692670:CDS 40.0%
! GTTCCATCTATCGCTGTTTC+AGG + chr4_4:61309630-61309649 Msa0692670:intron 45.0%
GCATCAGAAGTAGTCTTCAC+AGG - chr4_4:61310223-61310242 None:intergenic 45.0%
GCTGAAGACATAGTAACTGG+AGG + chr4_4:61310241-61310260 Msa0692670:CDS 45.0%
ACTGGAGGAAAGAGGAATGA+AGG + chr4_4:61310256-61310275 Msa0692670:CDS 45.0%
! CAGAACAGCTGATGAGTTGA+GGG + chr4_4:61310509-61310528 Msa0692670:CDS 45.0%
! GTTCCATCTATCGCTGTTTC+AGG + chr4_4:61309630-61309649 Msa0692670:intron 45.0%
GCATCAGAAGTAGTCTTCAC+AGG - chr4_4:61310223-61310242 None:intergenic 45.0%
GCTGAAGACATAGTAACTGG+AGG + chr4_4:61310241-61310260 Msa0692670:CDS 45.0%
ACTGGAGGAAAGAGGAATGA+AGG + chr4_4:61310256-61310275 Msa0692670:CDS 45.0%
! CAGAACAGCTGATGAGTTGA+GGG + chr4_4:61310509-61310528 Msa0692670:CDS 45.0%
!!! TAGATTTTTTTTTTTTTTTT+TGG + chr4_4:61310919-61310938 Msa0692670:exon 5.0%
!!! AGATTTTTTTTTTTTTTTTT+GGG + chr4_4:61310920-61310939 Msa0692670:exon 5.0%
!!! TAGATTTTTTTTTTTTTTTT+TGG + chr4_4:61310919-61310938 Msa0692670:exon 5.0%
!!! AGATTTTTTTTTTTTTTTTT+GGG + chr4_4:61310920-61310939 Msa0692670:exon 5.0%
CTGTAACTAACTTCGCCAGC+TGG - chr4_4:61309365-61309384 None:intergenic 50.0%
GAGCCTGAAACAGCGATAGA+TGG - chr4_4:61309636-61309655 None:intergenic 50.0%
GCAGAACAGCTGATGAGTTG+AGG + chr4_4:61310508-61310527 Msa0692670:CDS 50.0%
AGCTGGAAGCTTTGCTTGCT+CGG + chr4_4:61310821-61310840 Msa0692670:intron 50.0%
CTGTAACTAACTTCGCCAGC+TGG - chr4_4:61309365-61309384 None:intergenic 50.0%
GAGCCTGAAACAGCGATAGA+TGG - chr4_4:61309636-61309655 None:intergenic 50.0%
GCAGAACAGCTGATGAGTTG+AGG + chr4_4:61310508-61310527 Msa0692670:CDS 50.0%
AGCTGGAAGCTTTGCTTGCT+CGG + chr4_4:61310821-61310840 Msa0692670:intron 50.0%
TTATACTCGCTGCTGCCAGC+TGG + chr4_4:61309347-61309366 Msa0692670:five_prime_UTR 55.0%
GAGCAAGCAAAGCTTCCAGC+TGG - chr4_4:61310822-61310841 None:intergenic 55.0%
TTATACTCGCTGCTGCCAGC+TGG + chr4_4:61309347-61309366 Msa0692670:five_prime_UTR 55.0%
GAGCAAGCAAAGCTTCCAGC+TGG - chr4_4:61310822-61310841 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 61309347 61311129 61309347 ID=Msa0692670;Name=Msa0692670
chr4_4 mRNA 61309347 61311129 61309347 ID=Msa0692670-mRNA-1;Parent=Msa0692670;Name=Msa0692670-mRNA-1;_AED=0.09;_eAED=0.09;_QI=157|1|1|1|0.75|0.6|5|208|148
chr4_4 exon 61309347 61309493 61309347 ID=Msa0692670-mRNA-1:exon:16908;Parent=Msa0692670-mRNA-1
chr4_4 exon 61310180 61310277 61310180 ID=Msa0692670-mRNA-1:exon:16909;Parent=Msa0692670-mRNA-1
chr4_4 exon 61310397 61310530 61310397 ID=Msa0692670-mRNA-1:exon:16910;Parent=Msa0692670-mRNA-1
chr4_4 exon 61310607 61310732 61310607 ID=Msa0692670-mRNA-1:exon:16911;Parent=Msa0692670-mRNA-1
chr4_4 exon 61310823 61311129 61310823 ID=Msa0692670-mRNA-1:exon:16912;Parent=Msa0692670-mRNA-1
chr4_4 five_prime_UTR 61309347 61309493 61309347 ID=Msa0692670-mRNA-1:five_prime_utr;Parent=Msa0692670-mRNA-1
chr4_4 five_prime_UTR 61310180 61310189 61310180 ID=Msa0692670-mRNA-1:five_prime_utr;Parent=Msa0692670-mRNA-1
chr4_4 CDS 61310190 61310277 61310190 ID=Msa0692670-mRNA-1:cds;Parent=Msa0692670-mRNA-1
chr4_4 CDS 61310397 61310530 61310397 ID=Msa0692670-mRNA-1:cds;Parent=Msa0692670-mRNA-1
chr4_4 CDS 61310607 61310732 61310607 ID=Msa0692670-mRNA-1:cds;Parent=Msa0692670-mRNA-1
chr4_4 CDS 61310823 61310921 61310823 ID=Msa0692670-mRNA-1:cds;Parent=Msa0692670-mRNA-1
chr4_4 three_prime_UTR 61310922 61311129 61310922 ID=Msa0692670-mRNA-1:three_prime_utr;Parent=Msa0692670-mRNA-1
Gene Sequence

>Msa0692670

ATGGAGCCAAGTTCTGATAACACGAATAGGCCTGTGAAGACTACTTCTGATGCTGAAGACATAGTAACTGGAGGAAAGAGGAATGAAGGAAAACCTTGGATGCGATCTGATGAATACGTATTGATCAAAGCTGCGGATGAAATGAAATGTGACTGGGCAAGGATATTGACATCCAAAAAGTACTCTTATATATTCGCAGGCAGAACAGCTGATGAGTTGAGGGATAAATATGAAAATATGGAAAAGGATAAATATGAACAAATGCTACAAGATGCAAAAAAGAAACAACAGATGGAAGAAAGCTCAAGAGATGGCCTGATGCTTGTTAATAATGATAAAAACAAAATGCTGGAAGCTTTGCTTGCTCGGAATGAGTTGCTGAGAAAGACTTCAGAGAAAATGGACATTGTAGTTAATTACATGCAAGAAGAGGAAAAGGATGAATAG
Protein sequence

>Msa0692670

MEPSSDNTNRPVKTTSDAEDIVTGGKRNEGKPWMRSDEYVLIKAADEMKCDWARILTSKKYSYIFAGRTADELRDKYENMEKDKYEQMLQDAKKKQQMEESSRDGLMLVNNDKNKMLEALLARNELLRKTSEKMDIVVNYMQEEEKDE*