AlfalfaGEDB | Msa0144390

M. sativa cultivar zhongmu-4 / Msa0144390

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0144390	XP_039683773.1	86.538	208	21	2	246	447	548	754	1.18e-114	363
Msa0144390	XP_039683773.1	90.533	169	5	1	1	158	1	169	3.14e-95	313
Msa0144390	XP_039683773.1	91.667	120	4	1	158	271	385	504	9.90e-63	227

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0144390	sp\|Q9SZL8\|FRS5_ARATH	40.984	122	64	2	152	267	422	541	6.45e-23	105
Msa0144390	sp\|Q9SZL8\|FRS5_ARATH	35.758	165	83	5	27	169	59	222	2.10e-16	85.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0144390	A0A6A5MW98	54.517	321	98	6	159	438	101	414	7.69e-106	329

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TFs/TRs:

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Gene ID	Type	Classification
Msa0144390	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0144390	Msa1208310	0.809577	1.766080e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0144390	MtrunA17_Chr5g0448761	42.478	113	59	1	161	267	420	532	3.87e-23	102
Msa0144390	MtrunA17_Chr5g0448761	37.405	131	69	2	36	153	59	189	2.28e-17	84.7
Msa0144390	MtrunA17_Chr1g0202341	38.889	126	69	2	152	271	397	520	2.00e-21	97.4
Msa0144390	MtrunA17_Chr1g0202341	30.303	165	99	5	1	153	17	177	6.37e-13	70.9
Msa0144390	MtrunA17_Chr5g0448511	39.655	116	64	1	158	267	189	304	1.18e-19	91.7
Msa0144390	MtrunA17_Chr5g0441581	35.766	137	82	1	137	267	349	485	8.00e-19	89.4
Msa0144390	MtrunA17_Chr1g0196541	43.820	89	44	1	159	241	343	431	2.64e-18	87.8
Msa0144390	MtrunA17_Chr1g0207551	34.746	118	71	1	161	272	385	502	4.11e-18	87.4
Msa0144390	MtrunA17_Chr2g0296781	43.678	87	43	1	161	241	345	431	8.52e-17	83.2
Msa0144390	MtrunA17_Chr6g0477261	42.529	87	44	1	161	241	234	320	1.90e-16	82.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0144390	AT4G38180.1	40.984	122	64	2	152	267	422	541	6.56e-24	105
Msa0144390	AT4G38180.1	35.758	165	83	5	27	169	59	222	2.13e-17	85.5
Msa0144390	AT4G15090.1	37.288	118	68	1	161	272	405	522	1.04e-21	98.6
Msa0144390	AT4G15090.5	37.288	118	68	1	161	272	405	522	1.04e-21	98.6
Msa0144390	AT4G15090.3	37.288	118	68	1	161	272	405	522	1.04e-21	98.6
Msa0144390	AT4G15090.2	37.288	118	68	1	161	272	405	522	1.04e-21	98.6
Msa0144390	AT4G15090.4	37.288	118	68	1	161	272	405	522	1.04e-21	98.6
Msa0144390	AT4G38170.1	34.483	116	70	1	158	267	191	306	7.46e-19	89.4
Msa0144390	AT2G27110.2	35.669	157	87	5	152	302	369	517	2.98e-18	88.2
Msa0144390	AT2G27110.1	35.669	157	87	5	152	302	369	517	2.98e-18	88.2

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Find 58 sgRNAs with CRISPR-Local

Find 272 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAGCTTCTATTATAAAC+TGG	0.392497	1_4:+3855466	None:intergenic
AAAGAGAGGATTATGAAAGT+AGG	0.500092	1_4:-3855306	Msa0144390:CDS
AAGTGTTCTACTTCTCGAAC+TGG	0.426697	1_4:-3856399	Msa0144390:CDS
AATTCATTATATCTGATTTG+AGG	0.445916	1_4:-3854793	Msa0144390:CDS
ACAAAAGGGAGAAAGAAGGA+TGG	0.625355	1_4:-3854614	Msa0144390:CDS
ACAAACAAAAGGGAGAAAGA+AGG	0.601052	1_4:-3854618	Msa0144390:CDS
ACAACAATGAATGGCTTCAA+GGG	0.573168	1_4:-3855516	Msa0144390:CDS
ACGGACTGGACAACAATGAA+TGG	0.575271	1_4:-3855525	Msa0144390:CDS
AGAAAAGCATGTCATGAGTG+TGG	0.709074	1_4:-3854518	Msa0144390:CDS
AGCAAAGGTATTAGTTGATT+TGG	0.254141	1_4:-3854840	Msa0144390:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTAATTCCTCACCAACATT+AGG	+	chr1_4:3854547-3854566	None:intergenic	30.0%
TAAATAGTGAACCTTGTAAG+GGG	-	chr1_4:3854563-3854582	Msa0144390:CDS	30.0%
CAATTCATTTGCAAAAAGGA+TGG	-	chr1_4:3854624-3854643	Msa0144390:CDS	30.0%
GTCTTTGTATGTTGTAATGA+AGG	-	chr1_4:3854688-3854707	Msa0144390:CDS	30.0%
CAATTATTGCAAACAGAAAC+AGG	-	chr1_4:3855104-3855123	Msa0144390:intron	30.0%
ATTATTCAATCCAACAAACG+GGG	+	chr1_4:3855292-3855311	None:intergenic	30.0%
GATTATTCAATCCAACAAAC+GGG	+	chr1_4:3855293-3855312	None:intergenic	30.0%
TGTGTTGAAATTTGAAAAGG+TGG	-	chr1_4:3855821-3855840	Msa0144390:intron	30.0%
CTTAGAAGTAGAAAAAAGAG+AGG	-	chr1_4:3855854-3855873	Msa0144390:intron	30.0%
AAAGAGAGGATTATGAAAGT+AGG	-	chr1_4:3855868-3855887	Msa0144390:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	3854466	3856730	3854466	ID=Msa0144390;Name=Msa0144390
chr1_4	mRNA	3854466	3856730	3854466	ID=Msa0144390-mRNA-1;Parent=Msa0144390;Name=Msa0144390-mRNA-1;_AED=0.07;_eAED=0.12;_QI=0\|0\|0\|1\|0\|0\|5\|0\|447
chr1_4	exon	3856487	3856730	3856487	ID=Msa0144390-mRNA-1:exon:1617;Parent=Msa0144390-mRNA-1
chr1_4	exon	3856223	3856453	3856223	ID=Msa0144390-mRNA-1:exon:1616;Parent=Msa0144390-mRNA-1
chr1_4	exon	3855375	3855574	3855375	ID=Msa0144390-mRNA-1:exon:1615;Parent=Msa0144390-mRNA-1
chr1_4	exon	3855230	3855356	3855230	ID=Msa0144390-mRNA-1:exon:1614;Parent=Msa0144390-mRNA-1
chr1_4	exon	3854466	3855007	3854466	ID=Msa0144390-mRNA-1:exon:1613;Parent=Msa0144390-mRNA-1
chr1_4	CDS	3856487	3856730	3856487	ID=Msa0144390-mRNA-1:cds;Parent=Msa0144390-mRNA-1
chr1_4	CDS	3856223	3856453	3856223	ID=Msa0144390-mRNA-1:cds;Parent=Msa0144390-mRNA-1
chr1_4	CDS	3855375	3855574	3855375	ID=Msa0144390-mRNA-1:cds;Parent=Msa0144390-mRNA-1
chr1_4	CDS	3855230	3855356	3855230	ID=Msa0144390-mRNA-1:cds;Parent=Msa0144390-mRNA-1
chr1_4	CDS	3854466	3855007	3854466	ID=Msa0144390-mRNA-1:cds;Parent=Msa0144390-mRNA-1

Gene Sequence

>Msa0144390

ATGGATGGTAATGTTTTTGAAGAAGCCACACAGTCAACAAATGTGGAAATATCTGAAGAAGATATTTTAGCTGCTGCTCCTAATGTTGGTGAGGAATTAAATAGTGAACCTTGTAAGGGGATGGAATTCAAATCTGTGGAAAAGGTTAAAGAATTTTACAATTCATTTGCAAAAAGGATGGGTTTTGGAGTACGTGTTCGCTCAACTAAACCAAAGAGAGCTGTCTTTGTATGTTGTAATGAAGAAATCCAAGATGACACAAATCAAACTAAAAGAAAGTGTTCTACTTCTCGAACTGGTTGTCTAGCCACACTTGTTGTTAAAAGAGGTACATTAGAAGGAAATTGGATTATATGTTCATTTAAGAATGATCATAACCATCCCATGGTTAGCCCTAAAAGTGTGTCTTATATGCGGTGTCATAAAAGCATGAATGGTGCTGCAAAAAGTCTTGTTGAACATTTTAAGGAAGAAGAAGACTGGAGCCGATTAATGATTGAGTACGGACTGGACAACAATGAATGGCTTCAAGGGTTATATAAAATTAGAGAATCTTGGATACCAGTTTATAATAGAAGCTGTTTTTTTGCTGGGATGAATACAAGTCAAAGAAGTGAGAGCATCAATGCGTTTTTTGATTCTTTTGTGAATGCAACTACAACATTGCAAGAATTTGTGGTTGATAGCCGCTTAGAAGTAGAAAAAAGAGAGGATTATGAAAGTAGGCATAAAAAACGCATATTGTCTACAGGGTCAAAACTTGAGGAGCACGCTGCGTCTATTTATTCGAGAAACATTTTTGGTATCACTCAAATTCCTGACCATTTTATTTTGCAGCGTTGGACTAAAAATGCTAACAAAAGCATTGAAGTTTGTGATACGACGAGTAATTTTGAAGGTCAAAATAGCACGCCAAAGATATTAAGAAGGATGCATGCTCAACAAGAAGCAAAGGTATTAGTTGATTTGGCTGAAGAGTCTGATGAGATATACAAATTCATTATATCTGATTTGAGGCGCACTCGTATGGCAGCCATTGCAATGAAGAAATCAAATTTAGCTATCAGTGATGGAGTTTCTCCTTTGGAATCTGGCCAAAATGTCAACCAAAATTGCTTGTCGGAGGAAGAAAGTGAACCAGAATTGACTATTAGAAATCCACGCAATGCACAAACAAAAGGGAGAAAGAAGGATGGACAGAAAAACACTCAAAATTGTAGATTCAAAAGTGGAATTGAACTGTCACATGCTCAACAAGTAGCAGTCAAAAGAAAAGCATGTCATGAGTGTGGTGAATCTGGTCACAACAGCAGAACTTGCAAGGAAAGAAATCAAAATGATTAA

Protein sequence

>Msa0144390

MDGNVFEEATQSTNVEISEEDILAAAPNVGEELNSEPCKGMEFKSVEKVKEFYNSFAKRMGFGVRVRSTKPKRAVFVCCNEEIQDDTNQTKRKCSTSRTGCLATLVVKRGTLEGNWIICSFKNDHNHPMVSPKSVSYMRCHKSMNGAAKSLVEHFKEEEDWSRLMIEYGLDNNEWLQGLYKIRESWIPVYNRSCFFAGMNTSQRSESINAFFDSFVNATTTLQEFVVDSRLEVEKREDYESRHKKRILSTGSKLEEHAASIYSRNIFGITQIPDHFILQRWTKNANKSIEVCDTTSNFEGQNSTPKILRRMHAQQEAKVLVDLAEESDEIYKFIISDLRRTRMAAIAMKKSNLAISDGVSPLESGQNVNQNCLSEEESEPELTIRNPRNAQTKGRKKDGQKNTQNCRFKSGIELSHAQQVAVKRKACHECGESGHNSRTCKERNQND*