AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0145820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0145820 MtrunA17_Chr1g0155081 95.833 312 12 1 1 311 1 312 0.0 618
Msa0145820 MtrunA17_Chr4g0034951 50.171 293 126 6 30 310 26 310 4.90e-94 281
Msa0145820 MtrunA17_Chr8g0348371 42.466 292 146 10 37 310 15 302 7.00e-60 193
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0145820 AT1G75180.1 56.631 279 101 6 48 311 42 315 1.42e-99 295
Msa0145820 AT1G75180.3 56.631 279 101 6 48 311 42 315 1.42e-99 295
Msa0145820 AT1G19400.1 51.645 304 123 8 20 311 12 303 2.41e-99 295
Msa0145820 AT1G19400.3 51.645 304 123 8 20 311 12 303 2.41e-99 295
Msa0145820 AT1G19400.2 51.645 304 123 8 20 311 12 303 2.41e-99 295
Msa0145820 AT1G19400.4 50.661 227 94 5 11 226 7 226 1.77e-70 219
Msa0145820 AT1G75180.2 55.263 190 70 3 48 223 42 230 7.28e-65 204
Msa0145820 AT1G75180.4 55.263 190 70 3 48 223 42 230 1.20e-63 202
Msa0145820 AT1G01500.1 43.151 292 137 13 43 311 42 327 3.98e-60 195

Find 80 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTACTTATCTGTGGAAAATT+TGG 0.104827 1_4:+5549143 Msa0145820:three_prime_UTR
ATTTCAGATGTTTCATATTC+TGG 0.154223 1_4:-5546990 None:intergenic
TGACTACTAAAGAGAAAATT+TGG 0.167128 1_4:-5549385 None:intergenic
TCGAGTTTGTACAATATTTC+AGG 0.240366 1_4:+5546214 Msa0145820:five_prime_UTR
AAAAGTGTATCAGGACTTAA+AGG 0.253465 1_4:-5546564 None:intergenic
ACAATCAACGAAACAGTTAA+AGG 0.256351 1_4:-5546255 None:intergenic
AGTGTCCTAGTTAAGATTAT+AGG 0.277513 1_4:-5546912 None:intergenic
ACAGATAGTATAAGAATAAA+TGG 0.288585 1_4:+5546690 Msa0145820:CDS
TATGTTGCTGGTTGTTTCTC+AGG 0.293158 1_4:+5546885 Msa0145820:CDS
GCAACATAAACCTCAATTTC+AGG 0.293507 1_4:-5546870 None:intergenic
TTGTTGTAATGCTCTTCTTC+TGG 0.302320 1_4:-5548789 None:intergenic
TCTTTGTAGGAGTTGGTATA+GGG 0.323141 1_4:+5548910 Msa0145820:CDS
ATGATAATGAGAAGAGATAT+TGG 0.323687 1_4:+5546781 Msa0145820:CDS
AAAAGTTTAATTGTTGTCAT+TGG 0.330187 1_4:+5549257 Msa0145820:three_prime_UTR
ATTGGACTTGGCATCTTTGT+AGG 0.336752 1_4:+5548897 Msa0145820:CDS
GAAATTGAGGTTTATGTTGC+TGG 0.339747 1_4:+5546873 Msa0145820:CDS
TGTTTCGTTGATTGTGAAAC+AGG 0.345089 1_4:+5546263 Msa0145820:five_prime_UTR
TGAAACTACGAGTAGTTCTT+TGG 0.346111 1_4:-5548956 None:intergenic
ATCTTTGTAGGAGTTGGTAT+AGG 0.360152 1_4:+5548909 Msa0145820:CDS
ATTGCAGGAAGAAACATAAT+AGG 0.362814 1_4:+5546943 Msa0145820:CDS
ATTTCACAGTAAATTCTTGT+TGG 0.364309 1_4:+5549416 Msa0145820:three_prime_UTR
TATGAATTGTGAAACAGAAA+TGG 0.385595 1_4:+5546806 Msa0145820:CDS
TTTACCTCTAAAAGTGTATC+AGG 0.386768 1_4:-5546573 None:intergenic
CTTGGCATCTTTGTAGGAGT+TGG 0.397784 1_4:+5548903 Msa0145820:CDS
GTAAGAGTTGGTGTTGGAAT+TGG 0.400841 1_4:+5548879 Msa0145820:CDS
AAATTAGAATGAAAATGAAA+TGG 0.403061 1_4:-5546042 None:intergenic
AATAAATTTCATCTTACAAA+AGG 0.416697 1_4:-5549003 None:intergenic
AAGATTCTTCAATTCTAAGA+AGG 0.437779 1_4:+5546625 Msa0145820:CDS
ATTTCTTTAAAAGCCTTTGA+AGG 0.439617 1_4:-5549206 None:intergenic
TGTTTCTGTTCCTGAAATTG+AGG 0.445193 1_4:+5546860 Msa0145820:CDS
GGACACTGCAGATCAATTGC+AGG 0.447539 1_4:+5546928 Msa0145820:CDS
TTAATGCATGATGGATCATA+AGG 0.452740 1_4:-5546435 None:intergenic
ACACAAAATATGAAATCTAA+AGG 0.457706 1_4:-5546162 None:intergenic
TATCTATATATCCTTCCCTT+TGG 0.458465 1_4:-5548821 None:intergenic
ATGAAATCTAAAGGGAAGAA+AGG 0.463115 1_4:-5546153 None:intergenic
TGGAAGATGGTGAGCTATCA+TGG 0.463930 1_4:+5548844 Msa0145820:CDS
AAGGGAAGGATATATAGATA+TGG 0.466582 1_4:+5548824 Msa0145820:CDS
TGGTATAGGGGTCAGCCTCT+TGG 0.472718 1_4:+5548923 Msa0145820:CDS
CACAAAATATGAAATCTAAA+GGG 0.476086 1_4:-5546161 None:intergenic
ATGAATTGTGAAACAGAAAT+GGG 0.487805 1_4:+5546807 Msa0145820:CDS
GAATGAAAATGAAATGGAAA+AGG 0.502375 1_4:-5546036 None:intergenic
GATGTTTGTGATCATGATCA+TGG 0.514929 1_4:+5547020 Msa0145820:CDS
AAAGAGAACTTGAGCGAAGG+TGG 0.522042 1_4:-5546119 None:intergenic
TTTCTTTAAAAGCCTTTGAA+GGG 0.528421 1_4:-5549205 None:intergenic
AATGATAATTTAATGCATGA+TGG 0.535072 1_4:-5546444 None:intergenic
ACAGAAATGGGACCTAATAG+TGG 0.538208 1_4:+5546819 Msa0145820:CDS
GTTGGTGTTGGAATTGGACT+TGG 0.540231 1_4:+5548885 Msa0145820:CDS
CAACAACATGTATTGCCAAA+GGG 0.547747 1_4:+5548806 Msa0145820:CDS
GAGCTATCATGGTTCAATGC+TGG 0.554903 1_4:+5548855 Msa0145820:CDS
TGATCATGGACTTGTTTCAC+AGG 0.556098 1_4:+5547034 Msa0145820:CDS
GGATATATAGATATGGAAGA+TGG 0.561870 1_4:+5548831 Msa0145820:CDS
ACTAGGTAGTGCGTACGGTA+TGG 0.566871 1_4:+5549060 Msa0145820:three_prime_UTR
ATGAATGTGGTTCTATAGCT+AGG 0.569789 1_4:+5549299 Msa0145820:three_prime_UTR
GAAAGATTGTGTACTAAACA+AGG 0.572805 1_4:-5549172 None:intergenic
ACTACGAGTAGTTCTTTGGT+AGG 0.574661 1_4:-5548952 None:intergenic
AGATTCTTCAATTCTAAGAA+GGG 0.575943 1_4:+5546626 Msa0145820:CDS
CTTTGTAGGAGTTGGTATAG+GGG 0.584980 1_4:+5548911 Msa0145820:CDS
GAAAACCTATAATCTTAACT+AGG 0.587994 1_4:+5546907 Msa0145820:CDS
AACATGTATTGCCAAAGGGA+AGG 0.589506 1_4:+5548810 Msa0145820:CDS
ACAACAACATGTATTGCCAA+AGG 0.592557 1_4:+5548805 Msa0145820:CDS
TGATGAAGATATAGAAATTG+TGG 0.597506 1_4:-5546537 None:intergenic
TTCAGGCAAAATTACATCAG+CGG 0.600431 1_4:+5546231 Msa0145820:five_prime_UTR
AAAGGGCAACTACTTATCTG+TGG 0.604818 1_4:+5549134 Msa0145820:three_prime_UTR
AAGAAAGAGAACTTGAGCGA+AGG 0.610368 1_4:-5546122 None:intergenic
TATGATGCATCATCATGCAA+TGG 0.618243 1_4:+5546369 Msa0145820:CDS
CTACTCGTAGTTTCAAGAGG+AGG 0.625683 1_4:+5548964 Msa0145820:CDS
GAACTACTCGTAGTTTCAAG+AGG 0.629061 1_4:+5548961 Msa0145820:CDS
GATGCATCATCATGCAATGG+AGG 0.630369 1_4:+5546372 Msa0145820:CDS
CTACCACTAGGTAGTGCGTA+CGG 0.636113 1_4:+5549055 Msa0145820:three_prime_UTR
ACAACACTTCTGTATGAATG+TGG 0.643795 1_4:+5549286 Msa0145820:three_prime_UTR
GCTGGAGTAAGAGTTGGTGT+TGG 0.644565 1_4:+5548873 Msa0145820:CDS
TTATATTTAGGAATCAAACT+CGG 0.649610 1_4:-5549110 None:intergenic
AAAGATTGTGTACTAAACAA+GGG 0.649643 1_4:-5549171 None:intergenic
TTCAATGCTGGAGTAAGAGT+TGG 0.649924 1_4:+5548867 Msa0145820:CDS
TAATAGGAAAGTGTGCATGT+TGG 0.650786 1_4:+5546959 Msa0145820:CDS
AAGATATAGAAATTGTGGTG+TGG 0.664213 1_4:-5546532 None:intergenic
TCAATTACTTATCTACCACT+AGG 0.665223 1_4:+5549043 Msa0145820:three_prime_UTR
AAAACTCTTACATCAAACCA+AGG 0.678998 1_4:-5546474 None:intergenic
TTTGGTAGGAACGTGCCAAG+AGG 0.697390 1_4:-5548938 None:intergenic
ATACCGTACGCACTACCTAG+TGG 0.715828 1_4:-5549058 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGCATATATATTTTTTATTT+AGG + chr1_4:5547997-5548016 Msa0145820:intron 10.0%
!! AAATTAGAATGAAAATGAAA+TGG - chr1_4:5546045-5546064 None:intergenic 15.0%
!! ACAAGAAAAACAAATTAAAA+AGG - chr1_4:5546188-5546207 None:intergenic 15.0%
!!! ATTAACAATTTTAGTGATTT+TGG + chr1_4:5546326-5546345 Msa0145820:five_prime_UTR 15.0%
!!! TATTAATGTTATCTATGTTT+TGG + chr1_4:5547461-5547480 Msa0145820:intron 15.0%
!!! ACATATCTTTTATTAACTAT+TGG + chr1_4:5547573-5547592 Msa0145820:intron 15.0%
!!! CATATCTTTTATTAACTATT+GGG + chr1_4:5547574-5547593 Msa0145820:intron 15.0%
!! ACTATCTATTAGATTAAATT+TGG + chr1_4:5547968-5547987 Msa0145820:intron 15.0%
!!! TTCATTTATGTTTTAATCTA+TGG + chr1_4:5548494-5548513 Msa0145820:intron 15.0%
!! ACATATATCTAGTTATAAAA+TGG + chr1_4:5548667-5548686 Msa0145820:intron 15.0%
!! AATAAATTTCATCTTACAAA+AGG - chr1_4:5549006-5549025 None:intergenic 15.0%
!! TTGATTCCTAAATATAAAAA+AGG + chr1_4:5549116-5549135 Msa0145820:three_prime_UTR 15.0%
!! TGATTCCTAAATATAAAAAA+GGG + chr1_4:5549117-5549136 Msa0145820:three_prime_UTR 15.0%
!! CACAAAATATGAAATCTAAA+GGG - chr1_4:5546164-5546183 None:intergenic 20.0%
!! ACACAAAATATGAAATCTAA+AGG - chr1_4:5546165-5546184 None:intergenic 20.0%
!! AATGATAATTTAATGCATGA+TGG - chr1_4:5546447-5546466 None:intergenic 20.0%
!!! AATTATCATTTTCATTACCT+TGG + chr1_4:5546457-5546476 Msa0145820:CDS 20.0%
!! ACAGATAGTATAAGAATAAA+TGG + chr1_4:5546690-5546709 Msa0145820:CDS 20.0%
!!! TATCATAATTTTCACTATCA+TGG + chr1_4:5547247-5547266 Msa0145820:intron 20.0%
!!! ATCATAATTTTCACTATCAT+GGG + chr1_4:5547248-5547267 Msa0145820:intron 20.0%
!!! TGACTAATTGACTATATTTT+CGG + chr1_4:5547332-5547351 Msa0145820:intron 20.0%
!! AATTTAGTACCTTTAGATAT+TGG + chr1_4:5547491-5547510 Msa0145820:intron 20.0%
!! AATAAAAGATATGTGTATTG+TGG - chr1_4:5547567-5547586 None:intergenic 20.0%
!!! TTTTGTTTATATTTGAGACA+GGG - chr1_4:5547762-5547781 None:intergenic 20.0%
!!! TTTTTGTTTATATTTGAGAC+AGG - chr1_4:5547763-5547782 None:intergenic 20.0%
!!! TATTTTGTCAAAAAACCTTA+TGG - chr1_4:5548177-5548196 None:intergenic 20.0%
!!! TTTTAATCTATGGATTTACT+TGG + chr1_4:5548504-5548523 Msa0145820:intron 20.0%
!! TTTATGCATTAATCTATACA+TGG + chr1_4:5548532-5548551 Msa0145820:intron 20.0%
!!! AAATTTTACAGATGCTTAAA+AGG - chr1_4:5548597-5548616 None:intergenic 20.0%
!! AATTTATTTACCATGTTACT+TGG + chr1_4:5548612-5548631 Msa0145820:intron 20.0%
!! TTATATTTAGGAATCAAACT+CGG - chr1_4:5549113-5549132 None:intergenic 20.0%
!! AAAAGTTTAATTGTTGTCAT+TGG + chr1_4:5549257-5549276 Msa0145820:three_prime_UTR 20.0%
! GAATGAAAATGAAATGGAAA+AGG - chr1_4:5546039-5546058 None:intergenic 25.0%
! TACAAGTCACAAGATAAAAA+TGG - chr1_4:5546095-5546114 None:intergenic 25.0%
! AAAAATGATGTAGACTTTGA+TGG + chr1_4:5546303-5546322 Msa0145820:five_prime_UTR 25.0%
!!! GTTTTTTATGTAAGAGTTAG+TGG + chr1_4:5546492-5546511 Msa0145820:CDS 25.0%
! TGATGAAGATATAGAAATTG+TGG - chr1_4:5546540-5546559 None:intergenic 25.0%
!! AAGATTCTTCAATTCTAAGA+AGG + chr1_4:5546625-5546644 Msa0145820:CDS 25.0%
!! AGATTCTTCAATTCTAAGAA+GGG + chr1_4:5546626-5546645 Msa0145820:CDS 25.0%
!! ATGATAATGAGAAGAGATAT+TGG + chr1_4:5546781-5546800 Msa0145820:CDS 25.0%
! TATGAATTGTGAAACAGAAA+TGG + chr1_4:5546806-5546825 Msa0145820:CDS 25.0%
! ATGAATTGTGAAACAGAAAT+GGG + chr1_4:5546807-5546826 Msa0145820:CDS 25.0%
! GAAAACCTATAATCTTAACT+AGG + chr1_4:5546907-5546926 Msa0145820:CDS 25.0%
! ATTTCAGATGTTTCATATTC+TGG - chr1_4:5546993-5547012 None:intergenic 25.0%
! TATTATCCACAAAAATTGCA+TGG - chr1_4:5547292-5547311 None:intergenic 25.0%
!! ATTGACTATATTTTCGGAAT+TGG + chr1_4:5547338-5547357 Msa0145820:intron 25.0%
! CAAGAAAACAGTATTATCAA+GGG - chr1_4:5547382-5547401 None:intergenic 25.0%
! ACAAGAAAACAGTATTATCA+AGG - chr1_4:5547383-5547402 None:intergenic 25.0%
!!! TCTAAAGGTACTAAATTAGA+GGG - chr1_4:5547488-5547507 None:intergenic 25.0%
!!! ATCTAAAGGTACTAAATTAG+AGG - chr1_4:5547489-5547508 None:intergenic 25.0%
! CAAATTTGTACCATAATAGA+AGG - chr1_4:5548110-5548129 None:intergenic 25.0%
! AAATAAATGGGCCAAATATA+GGG - chr1_4:5548420-5548439 None:intergenic 25.0%
!! ATCTATACATGGATTTTCTT+AGG + chr1_4:5548543-5548562 Msa0145820:intron 25.0%
! AACTAGATATATGTTGTTGA+AGG - chr1_4:5548661-5548680 None:intergenic 25.0%
!!! TAGTAATAACTTAGCTTGTT+TGG + chr1_4:5548729-5548748 Msa0145820:intron 25.0%
!!! AGTTGCCCTTTTTTATATTT+AGG - chr1_4:5549125-5549144 None:intergenic 25.0%
! AAAGATTGTGTACTAAACAA+GGG - chr1_4:5549174-5549193 None:intergenic 25.0%
!! TTTCTTTAAAAGCCTTTGAA+GGG - chr1_4:5549208-5549227 None:intergenic 25.0%
!! ATTTCTTTAAAAGCCTTTGA+AGG - chr1_4:5549209-5549228 None:intergenic 25.0%
!!! ATGTTCTTGTTTTGTTTTGA+AGG + chr1_4:5549227-5549246 Msa0145820:three_prime_UTR 25.0%
!! AATTTTGTGTCAATCTTCAA+TGG - chr1_4:5549338-5549357 None:intergenic 25.0%
! TGACTACTAAAGAGAAAATT+TGG - chr1_4:5549388-5549407 None:intergenic 25.0%
! ATTTCACAGTAAATTCTTGT+TGG + chr1_4:5549416-5549435 Msa0145820:three_prime_UTR 25.0%
ATGAAATCTAAAGGGAAGAA+AGG - chr1_4:5546156-5546175 None:intergenic 30.0%
TCGAGTTTGTACAATATTTC+AGG + chr1_4:5546214-5546233 Msa0145820:five_prime_UTR 30.0%
ACAATCAACGAAACAGTTAA+AGG - chr1_4:5546258-5546277 None:intergenic 30.0%
! AGTGATTTTGGATATGAAGA+TGG + chr1_4:5546338-5546357 Msa0145820:exon 30.0%
! GTGATTTTGGATATGAAGAT+GGG + chr1_4:5546339-5546358 Msa0145820:exon 30.0%
!! TTAATGCATGATGGATCATA+AGG - chr1_4:5546438-5546457 None:intergenic 30.0%
AAAACTCTTACATCAAACCA+AGG - chr1_4:5546477-5546496 None:intergenic 30.0%
AAGATATAGAAATTGTGGTG+TGG - chr1_4:5546535-5546554 None:intergenic 30.0%
! AAAAGTGTATCAGGACTTAA+AGG - chr1_4:5546567-5546586 None:intergenic 30.0%
! TTTACCTCTAAAAGTGTATC+AGG - chr1_4:5546576-5546595 None:intergenic 30.0%
!! GATACACTTTTAGAGGTAAA+TGG + chr1_4:5546576-5546595 Msa0145820:CDS 30.0%
! CCTTTGAAAAAACCACTATT+AGG - chr1_4:5546834-5546853 None:intergenic 30.0%
!!! CCTAATAGTGGTTTTTTCAA+AGG + chr1_4:5546831-5546850 Msa0145820:CDS 30.0%
AGTGTCCTAGTTAAGATTAT+AGG - chr1_4:5546915-5546934 None:intergenic 30.0%
ATTGCAGGAAGAAACATAAT+AGG + chr1_4:5546943-5546962 Msa0145820:CDS 30.0%
!! ACAACTTTTAATTGCGTGTT+TGG + chr1_4:5547066-5547085 Msa0145820:intron 30.0%
TATGAGTTTGATGAAAACAC+AGG + chr1_4:5547095-5547114 Msa0145820:intron 30.0%
ATGAGTTTGATGAAAACACA+GGG + chr1_4:5547096-5547115 Msa0145820:intron 30.0%
! AGCTTTTGCCAAAATAATGA+CGG + chr1_4:5547144-5547163 Msa0145820:intron 30.0%
AATTTGCGAAATGTTCCATT+TGG + chr1_4:5547213-5547232 Msa0145820:intron 30.0%
AATGTTCCATTTGGTCTATT+AGG + chr1_4:5547222-5547241 Msa0145820:intron 30.0%
ATGTTCCATTTGGTCTATTA+GGG + chr1_4:5547223-5547242 Msa0145820:intron 30.0%
!!! TTTTTGGGACCAATATCTAA+AGG - chr1_4:5547503-5547522 None:intergenic 30.0%
!!! ACTTCTAGGATAGGATTTTT+GGG - chr1_4:5547518-5547537 None:intergenic 30.0%
AATCTATTTACAAGGGACAT+TGG - chr1_4:5547639-5547658 None:intergenic 30.0%
AGGTCAGAATCTATTTACAA+GGG - chr1_4:5547646-5547665 None:intergenic 30.0%
AAGGTCAGAATCTATTTACA+AGG - chr1_4:5547647-5547666 None:intergenic 30.0%
GACACAACATACAGAAAATA+AGG - chr1_4:5547704-5547723 None:intergenic 30.0%
! AAGGAGTAGATTGAAAGTTT+TGG - chr1_4:5548091-5548110 None:intergenic 30.0%
TCAATCTACTCCTTCTATTA+TGG + chr1_4:5548097-5548116 Msa0145820:intron 30.0%
GAGAAAAGGTAGTTATAAGA+AGG - chr1_4:5548232-5548251 None:intergenic 30.0%
! ACCTTTTCTCTCAAGTATAT+TGG + chr1_4:5548242-5548261 Msa0145820:intron 30.0%
ACTGTAACCAAATACATACA+TGG + chr1_4:5548276-5548295 Msa0145820:intron 30.0%
TGAACAACCATGTATGTATT+TGG - chr1_4:5548286-5548305 None:intergenic 30.0%
CAAATAAATGGGCCAAATAT+AGG - chr1_4:5548421-5548440 None:intergenic 30.0%
TGGACCTTAGACAAATAAAT+GGG - chr1_4:5548432-5548451 None:intergenic 30.0%
TTGGACCTTAGACAAATAAA+TGG - chr1_4:5548433-5548452 None:intergenic 30.0%
!! TGCTTAAAAGGTTTGCTAAA+TGG - chr1_4:5548585-5548604 None:intergenic 30.0%
CAAATTAAGTCCAAGTAACA+TGG - chr1_4:5548625-5548644 None:intergenic 30.0%
!!! TAACTTAGCTTGTTTGGTTT+TGG + chr1_4:5548735-5548754 Msa0145820:intron 30.0%
TATCTATATATCCTTCCCTT+TGG - chr1_4:5548824-5548843 None:intergenic 30.0%
AAGGGAAGGATATATAGATA+TGG + chr1_4:5548824-5548843 Msa0145820:CDS 30.0%
GGATATATAGATATGGAAGA+TGG + chr1_4:5548831-5548850 Msa0145820:CDS 30.0%
TCAATTACTTATCTACCACT+AGG + chr1_4:5549043-5549062 Msa0145820:three_prime_UTR 30.0%
CTACTTATCTGTGGAAAATT+TGG + chr1_4:5549143-5549162 Msa0145820:three_prime_UTR 30.0%
GAAAGATTGTGTACTAAACA+AGG - chr1_4:5549175-5549194 None:intergenic 30.0%
TTCAGGCAAAATTACATCAG+CGG + chr1_4:5546231-5546250 Msa0145820:five_prime_UTR 35.0%
! TGTTTCGTTGATTGTGAAAC+AGG + chr1_4:5546263-5546282 Msa0145820:five_prime_UTR 35.0%
! ATTTTGGATATGAAGATGGG+AGG + chr1_4:5546342-5546361 Msa0145820:exon 35.0%
TATGATGCATCATCATGCAA+TGG + chr1_4:5546369-5546388 Msa0145820:CDS 35.0%
! AAGTCCTGATACACTTTTAG+AGG + chr1_4:5546569-5546588 Msa0145820:CDS 35.0%
! TGTTTCTGTTCCTGAAATTG+AGG + chr1_4:5546860-5546879 Msa0145820:CDS 35.0%
GCAACATAAACCTCAATTTC+AGG - chr1_4:5546873-5546892 None:intergenic 35.0%
GAAATTGAGGTTTATGTTGC+TGG + chr1_4:5546873-5546892 Msa0145820:CDS 35.0%
! TAATAGGAAAGTGTGCATGT+TGG + chr1_4:5546959-5546978 Msa0145820:CDS 35.0%
GATGTTTGTGATCATGATCA+TGG + chr1_4:5547020-5547039 Msa0145820:CDS 35.0%
TTCGCAAATTAGTGCATGTT+TGG - chr1_4:5547203-5547222 None:intergenic 35.0%
GATATCCCTAATAGACCAAA+TGG - chr1_4:5547231-5547250 None:intergenic 35.0%
! CTTTTGCCATGCAATTTTTG+TGG + chr1_4:5547283-5547302 Msa0145820:intron 35.0%
TTCGGAATTGGAATAAGATG+TGG + chr1_4:5547350-5547369 Msa0145820:intron 35.0%
!!! CACTTCTAGGATAGGATTTT+TGG - chr1_4:5547519-5547538 None:intergenic 35.0%
ACCAGTACTTGTCCTTTAAT+GGG - chr1_4:5547671-5547690 None:intergenic 35.0%
CGTAATCATATGTATCGTGT+CGG + chr1_4:5547887-5547906 Msa0145820:intron 35.0%
CTTCTAGATGTACATTCATC+TGG - chr1_4:5548024-5548043 None:intergenic 35.0%
CAGATGAATGTACATCTAGA+AGG + chr1_4:5548022-5548041 Msa0145820:intron 35.0%
! GAAGGTGTGAGTTGTTAAAA+TGG + chr1_4:5548040-5548059 Msa0145820:intron 35.0%
GTTTAGACAGAGAGAGAATT+AGG - chr1_4:5548155-5548174 None:intergenic 35.0%
GCCAATATACTTGAGAGAAA+AGG - chr1_4:5548246-5548265 None:intergenic 35.0%
TACATACATGGTTGTTCAGT+AGG + chr1_4:5548288-5548307 Msa0145820:intron 35.0%
! CTTTCTGAAAAAAGTCGATC+GGG - chr1_4:5548347-5548366 None:intergenic 35.0%
! CCTTTCTGAAAAAAGTCGAT+CGG - chr1_4:5548348-5548367 None:intergenic 35.0%
AGAAAGGTGAGCAAATTTGT+GGG + chr1_4:5548361-5548380 Msa0145820:intron 35.0%
AGCAACCATCGAATACATAT+CGG + chr1_4:5548392-5548411 Msa0145820:intron 35.0%
TTGGCCCATTTATTTGTCTA+AGG + chr1_4:5548425-5548444 Msa0145820:intron 35.0%
!! ACTGTGTTAGGTATTGGATT+TGG - chr1_4:5548452-5548471 None:intergenic 35.0%
CTAAAAGCAGATACTGTGTT+AGG - chr1_4:5548464-5548483 None:intergenic 35.0%
GTTGTTGAAGGACTAATACT+AGG - chr1_4:5548649-5548668 None:intergenic 35.0%
!!! CTTGTTTGGTTTTGGTTCAT+AGG + chr1_4:5548743-5548762 Msa0145820:intron 35.0%
TTGTTGTAATGCTCTTCTTC+TGG - chr1_4:5548792-5548811 None:intergenic 35.0%
ACAACAACATGTATTGCCAA+AGG + chr1_4:5548805-5548824 Msa0145820:CDS 35.0%
CAACAACATGTATTGCCAAA+GGG + chr1_4:5548806-5548825 Msa0145820:CDS 35.0%
!! ATCTTTGTAGGAGTTGGTAT+AGG + chr1_4:5548909-5548928 Msa0145820:CDS 35.0%
!! TCTTTGTAGGAGTTGGTATA+GGG + chr1_4:5548910-5548929 Msa0145820:CDS 35.0%
TGAAACTACGAGTAGTTCTT+TGG - chr1_4:5548959-5548978 None:intergenic 35.0%
ACAACACTTCTGTATGAATG+TGG + chr1_4:5549286-5549305 Msa0145820:three_prime_UTR 35.0%
! ATGAATGTGGTTCTATAGCT+AGG + chr1_4:5549299-5549318 Msa0145820:three_prime_UTR 35.0%
AAGAAAGAGAACTTGAGCGA+AGG - chr1_4:5546125-5546144 None:intergenic 40.0%
ACAGAAATGGGACCTAATAG+TGG + chr1_4:5546819-5546838 Msa0145820:CDS 40.0%
!! TATGTTGCTGGTTGTTTCTC+AGG + chr1_4:5546885-5546904 Msa0145820:CDS 40.0%
! TGATCATGGACTTGTTTCAC+AGG + chr1_4:5547034-5547053 Msa0145820:CDS 40.0%
! ACAGTGTGCCGTCATTATTT+TGG - chr1_4:5547155-5547174 None:intergenic 40.0%
!! AACAGTCACACTTCTAGGAT+AGG - chr1_4:5547527-5547546 None:intergenic 40.0%
! TAGAAGTGTGACTGTTGCAT+GGG + chr1_4:5547531-5547550 Msa0145820:intron 40.0%
ATTCTGACCTTCCCCATTAA+AGG + chr1_4:5547656-5547675 Msa0145820:intron 40.0%
TACTTGTCCTTTAATGGGGA+AGG - chr1_4:5547666-5547685 None:intergenic 40.0%
CCAGTACTTGTCCTTTAATG+GGG - chr1_4:5547670-5547689 None:intergenic 40.0%
CACCAGTACTTGTCCTTTAA+TGG - chr1_4:5547672-5547691 None:intergenic 40.0%
!! TAAAGGACAAGTACTGGTGT+TGG + chr1_4:5547673-5547692 Msa0145820:intron 40.0%
!! AAAGGACAAGTACTGGTGTT+GGG + chr1_4:5547674-5547693 Msa0145820:intron 40.0%
ATGAAGCACAGACATAGACA+CGG + chr1_4:5547796-5547815 Msa0145820:intron 40.0%
!! TATGAAGCACTGATACTCCT+CGG - chr1_4:5547949-5547968 None:intergenic 40.0%
CTCTCTCTGTCTAAACCATA+AGG + chr1_4:5548159-5548178 Msa0145820:intron 40.0%
ATAACCAATGTCTCCCCTAA+TGG + chr1_4:5548197-5548216 Msa0145820:intron 40.0%
AAGGAGACTCTTACCATTAG+GGG - chr1_4:5548213-5548232 None:intergenic 40.0%
GAAGGAGACTCTTACCATTA+GGG - chr1_4:5548214-5548233 None:intergenic 40.0%
AGAAGGAGACTCTTACCATT+AGG - chr1_4:5548215-5548234 None:intergenic 40.0%
TTTGGTTACAGTTGCTGATG+TGG - chr1_4:5548268-5548287 None:intergenic 40.0%
!!! CCGATCGACTTTTTTCAGAA+AGG + chr1_4:5548345-5548364 Msa0145820:intron 40.0%
CAGAAAGGTGAGCAAATTTG+TGG + chr1_4:5548360-5548379 Msa0145820:intron 40.0%
GAAAGGTGAGCAAATTTGTG+GGG + chr1_4:5548362-5548381 Msa0145820:intron 40.0%
GTATTCGATGGTTGCTCAAT+AGG - chr1_4:5548388-5548407 None:intergenic 40.0%
! ACATATCGGTGCCCTATATT+TGG + chr1_4:5548406-5548425 Msa0145820:intron 40.0%
GCAGATACTGTGTTAGGTAT+TGG - chr1_4:5548458-5548477 None:intergenic 40.0%
AACATGTATTGCCAAAGGGA+AGG + chr1_4:5548810-5548829 Msa0145820:CDS 40.0%
TTCAATGCTGGAGTAAGAGT+TGG + chr1_4:5548867-5548886 Msa0145820:CDS 40.0%
!! GTAAGAGTTGGTGTTGGAAT+TGG + chr1_4:5548879-5548898 Msa0145820:CDS 40.0%
ATTGGACTTGGCATCTTTGT+AGG + chr1_4:5548897-5548916 Msa0145820:CDS 40.0%
!! CTTTGTAGGAGTTGGTATAG+GGG + chr1_4:5548911-5548930 Msa0145820:CDS 40.0%
ACTACGAGTAGTTCTTTGGT+AGG - chr1_4:5548955-5548974 None:intergenic 40.0%
GAACTACTCGTAGTTTCAAG+AGG + chr1_4:5548961-5548980 Msa0145820:CDS 40.0%
AAAGGGCAACTACTTATCTG+TGG + chr1_4:5549134-5549153 Msa0145820:three_prime_UTR 40.0%
! TCATTTTCTGTGCCCTTCAA+AGG + chr1_4:5549193-5549212 Msa0145820:three_prime_UTR 40.0%
AAAGAGAACTTGAGCGAAGG+TGG - chr1_4:5546122-5546141 None:intergenic 45.0%
GATGCATCATCATGCAATGG+AGG + chr1_4:5546372-5546391 Msa0145820:CDS 45.0%
GCATGTTTGGATTGACAGTG+AGG - chr1_4:5547190-5547209 None:intergenic 45.0%
CATGCAACAGTCACACTTCT+AGG - chr1_4:5547532-5547551 None:intergenic 45.0%
! CTAGAAGTGTGACTGTTGCA+TGG + chr1_4:5547530-5547549 Msa0145820:intron 45.0%
CCCCATTAAAGGACAAGTAC+TGG + chr1_4:5547667-5547686 Msa0145820:intron 45.0%
!! AAGGACAAGTACTGGTGTTG+GGG + chr1_4:5547675-5547694 Msa0145820:intron 45.0%
GATTAGACGTGTTCTGGTGT+CGG - chr1_4:5547927-5547946 None:intergenic 45.0%
CTTACCATTAGGGGAGACAT+TGG - chr1_4:5548204-5548223 None:intergenic 45.0%
CTCTCAATTTCGCTCCTGAT+CGG + chr1_4:5548312-5548331 Msa0145820:intron 45.0%
TGGAAGATGGTGAGCTATCA+TGG + chr1_4:5548844-5548863 Msa0145820:CDS 45.0%
GAGCTATCATGGTTCAATGC+TGG + chr1_4:5548855-5548874 Msa0145820:CDS 45.0%
!! GTTGGTGTTGGAATTGGACT+TGG + chr1_4:5548885-5548904 Msa0145820:CDS 45.0%
CTTGGCATCTTTGTAGGAGT+TGG + chr1_4:5548903-5548922 Msa0145820:CDS 45.0%
CTACTCGTAGTTTCAAGAGG+AGG + chr1_4:5548964-5548983 Msa0145820:CDS 45.0%
GGACACTGCAGATCAATTGC+AGG + chr1_4:5546928-5546947 Msa0145820:CDS 50.0%
ACAGACATAGACACGGACAC+CGG + chr1_4:5547803-5547822 Msa0145820:intron 50.0%
ACCAGAACACGTCTAATCCG+AGG + chr1_4:5547929-5547948 Msa0145820:intron 50.0%
! TCCTCGGATTAGACGTGTTC+TGG - chr1_4:5547933-5547952 None:intergenic 50.0%
CGATCAGGAGCGAAATTGAG+AGG - chr1_4:5548314-5548333 None:intergenic 50.0%
TCGGGAGTTACAAACCGATC+AGG - chr1_4:5548329-5548348 None:intergenic 50.0%
!! GGGCACCGATATGTATTCGA+TGG - chr1_4:5548400-5548419 None:intergenic 50.0%
!! GCTGGAGTAAGAGTTGGTGT+TGG + chr1_4:5548873-5548892 Msa0145820:CDS 50.0%
TTTGGTAGGAACGTGCCAAG+AGG - chr1_4:5548941-5548960 None:intergenic 50.0%
CTACCACTAGGTAGTGCGTA+CGG + chr1_4:5549055-5549074 Msa0145820:three_prime_UTR 50.0%
ATACCGTACGCACTACCTAG+TGG - chr1_4:5549061-5549080 None:intergenic 50.0%
ACTAGGTAGTGCGTACGGTA+TGG + chr1_4:5549060-5549079 Msa0145820:three_prime_UTR 50.0%
AGTGTGACTGTTGCATGGGC+AGG + chr1_4:5547535-5547554 Msa0145820:intron 55.0%
!! GACAAGTACTGGTGTTGGGG+AGG + chr1_4:5547678-5547697 Msa0145820:intron 55.0%
GTCAGTGTCTGTGTCGTGTC+CGG - chr1_4:5547825-5547844 None:intergenic 55.0%
TGGTATAGGGGTCAGCCTCT+TGG + chr1_4:5548923-5548942 Msa0145820:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 5546030 5549454 5546030 ID=Msa0145820;Name=Msa0145820
chr1_4 mRNA 5546030 5549454 5546030 ID=Msa0145820-mRNA-1;Parent=Msa0145820;Name=Msa0145820-mRNA-1;_AED=0.01;_eAED=0.01;_QI=321|1|1|1|1|1|2|459|311
chr1_4 exon 5546030 5547055 5546030 ID=Msa0145820-mRNA-1:exon:2303;Parent=Msa0145820-mRNA-1
chr1_4 exon 5548765 5549454 5548765 ID=Msa0145820-mRNA-1:exon:2304;Parent=Msa0145820-mRNA-1
chr1_4 five_prime_UTR 5546030 5546350 5546030 ID=Msa0145820-mRNA-1:five_prime_utr;Parent=Msa0145820-mRNA-1
chr1_4 CDS 5546351 5547055 5546351 ID=Msa0145820-mRNA-1:cds;Parent=Msa0145820-mRNA-1
chr1_4 CDS 5548765 5548995 5548765 ID=Msa0145820-mRNA-1:cds;Parent=Msa0145820-mRNA-1
chr1_4 three_prime_UTR 5548996 5549454 5548996 ID=Msa0145820-mRNA-1:three_prime_utr;Parent=Msa0145820-mRNA-1
Gene Sequence

>Msa0145820

ATGAAGATGGGAGGATCTTATGATGCATCATCATGCAATGGAGGAGAAGCAAGAAATAAAAGTCTTCAAATAATTGAATATTCACCTTATGATCCATCATGCATTAAATTATCATTTTCATTACCTTGGTTTGATGTAAGAGTTTTTTATGTAAGAGTTAGTGGAATTCAAGTGAATGAATCCACACCACAATTTCTATATCTTCATCATATACCTTTAAGTCCTGATACACTTTTAGAGGTAAATGGTGTTAGAAGTAACATGAATAGTGATGAAGATTCTTCAATTCTAAGAAGGGATAGAATTGATAAGAAATCTGAACAAGTCACTTTTGTGAATACAGATAGTATAAGAATAAATGGTAGCATGAAGTTTGAAGTTTGTGATAAAGATCAATGCATTCTCTCGTGTGTTCTTGAGATGAAAAGTGATGATAATGAGAAGAGATATTGGAGTATGAATTGTGAAACAGAAATGGGACCTAATAGTGGTTTTTTCAAAGGAAAACATGTTTCTGTTCCTGAAATTGAGGTTTATGTTGCTGGTTGTTTCTCAGGAAAACCTATAATCTTAACTAGGACACTGCAGATCAATTGCAGGAAGAAACATAATAGGAAAGTGTGCATGTTGGATGTGATTCCAGAATATGAAACATCTGAAATTAAGAAAGATGTTTGTGATCATGATCATGGACTTGTTTCACAGGTAGCTGAGTACAGAAGCTTCAAACCAGAAGAAGAGCATTACAACAACATGTATTGCCAAAGGGAAGGATATATAGATATGGAAGATGGTGAGCTATCATGGTTCAATGCTGGAGTAAGAGTTGGTGTTGGAATTGGACTTGGCATCTTTGTAGGAGTTGGTATAGGGGTCAGCCTCTTGGCACGTTCCTACCAAAGAACTACTCGTAGTTTCAAGAGGAGGTTCATATAA
Protein sequence

>Msa0145820

MKMGGSYDASSCNGGEARNKSLQIIEYSPYDPSCIKLSFSLPWFDVRVFYVRVSGIQVNESTPQFLYLHHIPLSPDTLLEVNGVRSNMNSDEDSSILRRDRIDKKSEQVTFVNTDSIRINGSMKFEVCDKDQCILSCVLEMKSDDNEKRYWSMNCETEMGPNSGFFKGKHVSVPEIEVYVAGCFSGKPIILTRTLQINCRKKHNRKVCMLDVIPEYETSEIKKDVCDHDHGLVSQVAEYRSFKPEEEHYNNMYCQREGYIDMEDGELSWFNAGVRVGVGIGLGIFVGVGIGVSLLARSYQRTTRSFKRRFI*