AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0058340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0058340 MtrunA17_Chr1g0155081 95.192 312 15 0 1 312 1 312 0.0 616
Msa0058340 MtrunA17_Chr4g0034951 49.320 294 128 7 30 311 26 310 1.36e-91 275
Msa0058340 MtrunA17_Chr8g0348371 41.924 291 150 9 37 311 15 302 1.41e-58 190
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0058340 AT1G75180.1 56.272 279 103 5 48 312 42 315 1.85e-99 295
Msa0058340 AT1G75180.3 56.272 279 103 5 48 312 42 315 1.85e-99 295
Msa0058340 AT1G19400.1 51.148 305 124 8 20 312 12 303 1.17e-97 290
Msa0058340 AT1G19400.3 51.148 305 124 8 20 312 12 303 1.17e-97 290
Msa0058340 AT1G19400.2 51.148 305 124 8 20 312 12 303 1.17e-97 290
Msa0058340 AT1G19400.4 50.442 226 94 5 11 225 7 225 6.99e-69 215
Msa0058340 AT1G75180.2 54.737 190 71 3 48 223 42 230 3.11e-63 200
Msa0058340 AT1G75180.4 54.737 190 71 3 48 223 42 230 4.49e-62 198
Msa0058340 AT1G01500.1 42.808 292 139 13 43 312 42 327 1.38e-58 191

Find 78 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTCAGATGTTTCATATTC+TGG 0.154223 1_2:-14675152 None:intergenic
TGACTACTAAAGAGAAAATT+TGG 0.167128 1_2:-14677748 None:intergenic
TTGTAATGCTCTTCTTCTTC+TGG 0.227081 1_2:-14677146 None:intergenic
TCGAGTTTGTACAATATTTC+AGG 0.240366 1_2:+14674376 Msa0058340:five_prime_UTR
AAAAGTGTATCAGGACTTAA+AGG 0.253465 1_2:-14674726 None:intergenic
ACAATCAACGAAACAGTTAA+AGG 0.256351 1_2:-14674417 None:intergenic
GCAACATAAACTTCAATTTC+AGG 0.260900 1_2:-14675032 None:intergenic
AGTGTCCTAGTTAAGATTAT+AGG 0.277513 1_2:-14675074 None:intergenic
ACAGATAGTATAAGAATAAA+TGG 0.288585 1_2:+14674852 Msa0058340:CDS
TCTTTGTAGGAGTTGGTATA+GGG 0.323141 1_2:+14677270 Msa0058340:CDS
ATGATAATGAGAAGAGATAT+TGG 0.323687 1_2:+14674943 Msa0058340:CDS
AAAAGTTTAATTGTTGTCAT+TGG 0.330187 1_2:+14677620 Msa0058340:three_prime_UTR
ATTGGACTTGGCATCTTTGT+AGG 0.336752 1_2:+14677257 Msa0058340:CDS
TGTTTCGTTGATTGTGAAAC+AGG 0.345089 1_2:+14674425 Msa0058340:five_prime_UTR
TGAAACTACGAGTAGTTCTT+TGG 0.346111 1_2:-14677316 None:intergenic
ATCTTTGTAGGAGTTGGTAT+AGG 0.360152 1_2:+14677269 Msa0058340:CDS
ATTGCAGGAAGAAACATAAT+AGG 0.362814 1_2:+14675105 Msa0058340:CDS
ATTTCACAGTAAATTCTTGT+TGG 0.364309 1_2:+14677779 Msa0058340:three_prime_UTR
CTACTTATCTGTGGAAAACT+TGG 0.373522 1_2:+14677506 Msa0058340:three_prime_UTR
GAAATTGAAGTTTATGTTGC+TGG 0.381875 1_2:+14675035 Msa0058340:CDS
TATGAATTGTGAAACAGAAA+TGG 0.385595 1_2:+14674968 Msa0058340:CDS
TTTACCTCTAAAAGTGTATC+AGG 0.386768 1_2:-14674735 None:intergenic
CTTGGCATCTTTGTAGGAGT+TGG 0.397784 1_2:+14677263 Msa0058340:CDS
GTAAGAGTTGGTGTTGGAAT+TGG 0.400841 1_2:+14677239 Msa0058340:CDS
AATAAATTTCATCTTACAAA+AGG 0.416697 1_2:-14677363 None:intergenic
AAGATTCTTCAATTCTAAGA+AGG 0.437779 1_2:+14674787 Msa0058340:CDS
ATTTCTTTAAAAGCCTTTGA+AGG 0.439617 1_2:-14677569 None:intergenic
AAATTAGAATGAAAATGAAA+TGG 0.444373 1_2:-14674204 None:intergenic
GGACACTGCAGATCAATTGC+AGG 0.447539 1_2:+14675090 Msa0058340:CDS
TTAATGCATGATGGATCATA+AGG 0.452740 1_2:-14674597 None:intergenic
ACACAAAATATGAAATCTAA+AGG 0.457706 1_2:-14674324 None:intergenic
TATCTATATATCCTTCCCTT+TGG 0.458465 1_2:-14677181 None:intergenic
ATGAAATCTAAAGGGAAGAA+AGG 0.463115 1_2:-14674315 None:intergenic
TGGAAGATGGTGAGCTATCA+TGG 0.463930 1_2:+14677204 Msa0058340:CDS
AAGGGAAGGATATATAGATA+TGG 0.466582 1_2:+14677184 Msa0058340:CDS
AAAGAGAACTTGAGTGAAGG+TGG 0.469788 1_2:-14674281 None:intergenic
TGGTATAGGGGTCAGCCTCT+TGG 0.472718 1_2:+14677283 Msa0058340:CDS
CACAAAATATGAAATCTAAA+GGG 0.476086 1_2:-14674323 None:intergenic
ATGAATTGTGAAACAGAAAT+GGG 0.487805 1_2:+14674969 Msa0058340:CDS
GAATGAAAATGAAATGGAAA+AGG 0.502375 1_2:-14674198 None:intergenic
GATGTTTGTGATCATGATCA+TGG 0.514929 1_2:+14675182 Msa0058340:CDS
TTTCTTTAAAAGCCTTTGAA+GGG 0.528421 1_2:-14677568 None:intergenic
AATGATAATTTAATGCATGA+TGG 0.535072 1_2:-14674606 None:intergenic
ACAGAAATGGGACCTAATAG+TGG 0.538208 1_2:+14674981 Msa0058340:CDS
GTTGGTGTTGGAATTGGACT+TGG 0.540231 1_2:+14677245 Msa0058340:CDS
CAACAACATGTATTGCCAAA+GGG 0.547747 1_2:+14677166 Msa0058340:CDS
GAGCTATCATGGTTCAATGC+TGG 0.554903 1_2:+14677215 Msa0058340:CDS
TGATCATGGACTTGTTTCAC+AGG 0.556098 1_2:+14675196 Msa0058340:CDS
GGATATATAGATATGGAAGA+TGG 0.561870 1_2:+14677191 Msa0058340:CDS
AAGAAAGAGAACTTGAGTGA+AGG 0.566707 1_2:-14674284 None:intergenic
ACTAGGTAGTGCGTACGGTA+TGG 0.566871 1_2:+14677423 Msa0058340:three_prime_UTR
ATGAATGTGGTTCTATAGCT+AGG 0.569789 1_2:+14677662 Msa0058340:three_prime_UTR
GAAAGATTGTGTACTAAACA+AGG 0.572805 1_2:-14677535 None:intergenic
ACTACGAGTAGTTCTTTGGT+AGG 0.574661 1_2:-14677312 None:intergenic
AGATTCTTCAATTCTAAGAA+GGG 0.575943 1_2:+14674788 Msa0058340:CDS
GAAAACCTATAATCTTAACT+AGG 0.581947 1_2:+14675069 Msa0058340:CDS
CTTTGTAGGAGTTGGTATAG+GGG 0.584980 1_2:+14677271 Msa0058340:CDS
AACATGTATTGCCAAAGGGA+AGG 0.589506 1_2:+14677170 Msa0058340:CDS
ACAACAACATGTATTGCCAA+AGG 0.592557 1_2:+14677165 Msa0058340:CDS
TGATGAAGATATAGAAATTG+TGG 0.597506 1_2:-14674699 None:intergenic
TTCAGGCAAAATTACATCAG+CGG 0.600431 1_2:+14674393 Msa0058340:five_prime_UTR
AAAGGGCAACTACTTATCTG+TGG 0.604818 1_2:+14677497 Msa0058340:three_prime_UTR
TATGATGCATCATCATGCAA+TGG 0.618243 1_2:+14674531 Msa0058340:CDS
CTACTCGTAGTTTCAAGAGG+AGG 0.625683 1_2:+14677324 Msa0058340:CDS
GAACTACTCGTAGTTTCAAG+AGG 0.629061 1_2:+14677321 Msa0058340:CDS
GATGCATCATCATGCAATGG+AGG 0.630369 1_2:+14674534 Msa0058340:CDS
CTACCACTAGGTAGTGCGTA+CGG 0.636113 1_2:+14677418 Msa0058340:three_prime_UTR
TTATATCTAGGAATCAAACT+CGG 0.636343 1_2:-14677473 None:intergenic
ACAACACTTCTGTATGAATG+TGG 0.643795 1_2:+14677649 Msa0058340:three_prime_UTR
GCTGGAGTAAGAGTTGGTGT+TGG 0.644565 1_2:+14677233 Msa0058340:CDS
AAAGATTGTGTACTAAACAA+GGG 0.649643 1_2:-14677534 None:intergenic
TTCAATGCTGGAGTAAGAGT+TGG 0.649924 1_2:+14677227 Msa0058340:CDS
TAATAGGAAAGTGTGCATGT+TGG 0.650786 1_2:+14675121 Msa0058340:CDS
AAGATATAGAAATTGTGGTG+TGG 0.664213 1_2:-14674694 None:intergenic
TCAATTACTTATCTACCACT+AGG 0.665223 1_2:+14677406 Msa0058340:three_prime_UTR
AAAACTCTTACATCAAACCA+AGG 0.678998 1_2:-14674636 None:intergenic
TTTGGTAGGAACGTGCCAAG+AGG 0.697390 1_2:-14677298 None:intergenic
ATACCGTACGCACTACCTAG+TGG 0.715828 1_2:-14677421 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATTTTTTATTTATATTT+AGG + chr1_2:14676339-14676358 Msa0058340:intron 0.0%
!!! ATAATTTTTAAAAATAAGTG+GGG + chr1_2:14675362-14675381 Msa0058340:intron 10.0%
!!! TAAAAAATTATTTTTTGTTG+TGG + chr1_2:14675453-14675472 Msa0058340:intron 10.0%
!!! AAAAAATTATTTTTTGTTGT+GGG + chr1_2:14675454-14675473 Msa0058340:intron 10.0%
!! AAATTAGAATGAAAATGAAA+TGG - chr1_2:14674207-14674226 None:intergenic 15.0%
!! ACAAGAAAAACAAATTAAAA+AGG - chr1_2:14674350-14674369 None:intergenic 15.0%
!!! ATTAACAATTTTAGTGATTT+TGG + chr1_2:14674488-14674507 Msa0058340:five_prime_UTR 15.0%
!!! ATTTTTAAAAATTATTAGGC+CGG - chr1_2:14675357-14675376 None:intergenic 15.0%
!!! GTTTTTTTAATTAGAATATG+AGG + chr1_2:14675409-14675428 Msa0058340:intron 15.0%
!!! AAAAATTATTTTTTGTTGTG+GGG + chr1_2:14675455-14675474 Msa0058340:intron 15.0%
!!! TATCATAATTTTCATATTCA+TGG + chr1_2:14675592-14675611 Msa0058340:intron 15.0%
!!! ATCATAATTTTCATATTCAT+GGG + chr1_2:14675593-14675612 Msa0058340:intron 15.0%
!!! TATTAATGTTATCTATGTTT+TGG + chr1_2:14675808-14675827 Msa0058340:intron 15.0%
!!! ACATATCTTTTATTAACTAT+TGG + chr1_2:14675923-14675942 Msa0058340:intron 15.0%
!!! CATATCTTTTATTAACTATT+GGG + chr1_2:14675924-14675943 Msa0058340:intron 15.0%
!! AATTATATTATTATCTGTGT+CGG - chr1_2:14676194-14676213 None:intergenic 15.0%
!! ACTATCTATTAGATTAAATT+TGG + chr1_2:14676304-14676323 Msa0058340:intron 15.0%
!! ATTGTAACTATATACATATA+TGG + chr1_2:14676639-14676658 Msa0058340:intron 15.0%
!! AATAAATTTCATCTTACAAA+AGG - chr1_2:14677366-14677385 None:intergenic 15.0%
!! CACAAAATATGAAATCTAAA+GGG - chr1_2:14674326-14674345 None:intergenic 20.0%
!! ACACAAAATATGAAATCTAA+AGG - chr1_2:14674327-14674346 None:intergenic 20.0%
!! AATGATAATTTAATGCATGA+TGG - chr1_2:14674609-14674628 None:intergenic 20.0%
!!! AATTATCATTTTCATTACCT+TGG + chr1_2:14674619-14674638 Msa0058340:CDS 20.0%
!! ACAGATAGTATAAGAATAAA+TGG + chr1_2:14674852-14674871 Msa0058340:CDS 20.0%
!! TTAAAACAAGCTTAAATACA+TGG + chr1_2:14675386-14675405 Msa0058340:intron 20.0%
!! TAAAACAAGCTTAAATACAT+GGG + chr1_2:14675387-14675406 Msa0058340:intron 20.0%
!!! TGACTAATTGACTATATTTT+CGG + chr1_2:14675679-14675698 Msa0058340:intron 20.0%
!! CAAAAAATCAGTATTATCAA+GGG - chr1_2:14675729-14675748 None:intergenic 20.0%
!! ACAAAAAATCAGTATTATCA+AGG - chr1_2:14675730-14675749 None:intergenic 20.0%
!! AATAAAAGATATGTGTATTG+TGG - chr1_2:14675917-14675936 None:intergenic 20.0%
!!! TTTTGTTTATATTTGAGACA+GGG - chr1_2:14676112-14676131 None:intergenic 20.0%
!!! TTTTTGTTTATATTTGAGAC+AGG - chr1_2:14676113-14676132 None:intergenic 20.0%
!! ATATAATTCAGTGTAATCAT+AGG + chr1_2:14676206-14676225 Msa0058340:intron 20.0%
!! CAAATTTGTATCATAATAGA+AGG - chr1_2:14676424-14676443 None:intergenic 20.0%
!!! TATTTTGTCAAAAAACCTTA+TGG - chr1_2:14676540-14676559 None:intergenic 20.0%
!!! TTATAAATGTGAGCAATTTT+AGG + chr1_2:14676722-14676741 Msa0058340:intron 20.0%
!!! TATAAATGTGAGCAATTTTA+GGG + chr1_2:14676723-14676742 Msa0058340:intron 20.0%
!! TTTATGCATTAATCTATACA+TGG + chr1_2:14676897-14676916 Msa0058340:intron 20.0%
!!! AAATTTTACAGATGCTTAAA+AGG - chr1_2:14676962-14676981 None:intergenic 20.0%
!! GCTTAACATAAAAGTTATTA+CGG - chr1_2:14677066-14677085 None:intergenic 20.0%
!! AACCAAACAAGTTATATAAT+AGG - chr1_2:14677090-14677109 None:intergenic 20.0%
!!! TATATAACTTGTTTGGTTTT+TGG + chr1_2:14677092-14677111 Msa0058340:intron 20.0%
!! TTGATTCCTAGATATAAAAA+AGG + chr1_2:14677479-14677498 Msa0058340:three_prime_UTR 20.0%
!! TGATTCCTAGATATAAAAAA+GGG + chr1_2:14677480-14677499 Msa0058340:three_prime_UTR 20.0%
!! AAAAGTTTAATTGTTGTCAT+TGG + chr1_2:14677620-14677639 Msa0058340:three_prime_UTR 20.0%
! GAATGAAAATGAAATGGAAA+AGG - chr1_2:14674201-14674220 None:intergenic 25.0%
! TACAAGTCACAAGATAAAAA+TGG - chr1_2:14674257-14674276 None:intergenic 25.0%
! AAAAATGATGTAGACTTTGA+TGG + chr1_2:14674465-14674484 Msa0058340:five_prime_UTR 25.0%
!!! GTTTTTTATGTAAGAGTTAG+TGG + chr1_2:14674654-14674673 Msa0058340:CDS 25.0%
! TGATGAAGATATAGAAATTG+TGG - chr1_2:14674702-14674721 None:intergenic 25.0%
!! AAGATTCTTCAATTCTAAGA+AGG + chr1_2:14674787-14674806 Msa0058340:CDS 25.0%
!! AGATTCTTCAATTCTAAGAA+GGG + chr1_2:14674788-14674807 Msa0058340:CDS 25.0%
!! ATGATAATGAGAAGAGATAT+TGG + chr1_2:14674943-14674962 Msa0058340:CDS 25.0%
! TATGAATTGTGAAACAGAAA+TGG + chr1_2:14674968-14674987 Msa0058340:CDS 25.0%
! ATGAATTGTGAAACAGAAAT+GGG + chr1_2:14674969-14674988 Msa0058340:CDS 25.0%
! GAAAACCTATAATCTTAACT+AGG + chr1_2:14675069-14675088 Msa0058340:CDS 25.0%
! ATTTCAGATGTTTCATATTC+TGG - chr1_2:14675155-14675174 None:intergenic 25.0%
!!! ACAACTTTTAAGTGCATTTA+TGG + chr1_2:14675228-14675247 Msa0058340:intron 25.0%
! TATTATCCACAAAAATTGCA+TGG - chr1_2:14675637-14675656 None:intergenic 25.0%
!! ATTGACTATATTTTCGGATT+TGG + chr1_2:14675685-14675704 Msa0058340:intron 25.0%
!!! TCTAAAGGTACTAAATTAGA+GGG - chr1_2:14675835-14675854 None:intergenic 25.0%
!!! ATCTAAAGGTACTAAATTAG+AGG - chr1_2:14675836-14675855 None:intergenic 25.0%
! TTAGTACCTTTAGATGATAT+TGG + chr1_2:14675841-14675860 Msa0058340:intron 25.0%
!! ACTTATAGGATAGGATTTTT+GGG - chr1_2:14675868-14675887 None:intergenic 25.0%
! AGTAATACGTTTATTTCCTT+AGG - chr1_2:14676469-14676488 None:intergenic 25.0%
! TACATATATGGTTACTCAAT+AGG + chr1_2:14676651-14676670 Msa0058340:intron 25.0%
! ATTTATAAAAATAGCCGATC+GGG - chr1_2:14676710-14676729 None:intergenic 25.0%
! CATTTATAAAAATAGCCGAT+CGG - chr1_2:14676711-14676730 None:intergenic 25.0%
!! ATAAATGTGAGCAATTTTAG+GGG + chr1_2:14676724-14676743 Msa0058340:intron 25.0%
!! ATCTATACATGGATTTTCTT+AGG + chr1_2:14676908-14676927 Msa0058340:intron 25.0%
!!! TGCTTAAAAGGTTTGTTAAA+TGG - chr1_2:14676950-14676969 None:intergenic 25.0%
! AATTTATCTACCATGTTACT+TGG + chr1_2:14676977-14676996 Msa0058340:intron 25.0%
!!! CACCTATTATATAACTTGTT+TGG + chr1_2:14677085-14677104 Msa0058340:intron 25.0%
! TTATATCTAGGAATCAAACT+CGG - chr1_2:14677476-14677495 None:intergenic 25.0%
! AAAGATTGTGTACTAAACAA+GGG - chr1_2:14677537-14677556 None:intergenic 25.0%
!! TTTCTTTAAAAGCCTTTGAA+GGG - chr1_2:14677571-14677590 None:intergenic 25.0%
!! ATTTCTTTAAAAGCCTTTGA+AGG - chr1_2:14677572-14677591 None:intergenic 25.0%
!!! ATGTTCTTGTTTTGTTTTGA+AGG + chr1_2:14677590-14677609 Msa0058340:three_prime_UTR 25.0%
!! AATTTTGTGTCAATCTTCAT+TGG - chr1_2:14677701-14677720 None:intergenic 25.0%
! TGACTACTAAAGAGAAAATT+TGG - chr1_2:14677751-14677770 None:intergenic 25.0%
! ATTTCACAGTAAATTCTTGT+TGG + chr1_2:14677779-14677798 Msa0058340:three_prime_UTR 25.0%
ATGAAATCTAAAGGGAAGAA+AGG - chr1_2:14674318-14674337 None:intergenic 30.0%
TCGAGTTTGTACAATATTTC+AGG + chr1_2:14674376-14674395 Msa0058340:five_prime_UTR 30.0%
ACAATCAACGAAACAGTTAA+AGG - chr1_2:14674420-14674439 None:intergenic 30.0%
! AGTGATTTTGGATATGAAGA+TGG + chr1_2:14674500-14674519 Msa0058340:exon 30.0%
! GTGATTTTGGATATGAAGAT+GGG + chr1_2:14674501-14674520 Msa0058340:exon 30.0%
!! TTAATGCATGATGGATCATA+AGG - chr1_2:14674600-14674619 None:intergenic 30.0%
AAAACTCTTACATCAAACCA+AGG - chr1_2:14674639-14674658 None:intergenic 30.0%
AAGATATAGAAATTGTGGTG+TGG - chr1_2:14674697-14674716 None:intergenic 30.0%
! AAAAGTGTATCAGGACTTAA+AGG - chr1_2:14674729-14674748 None:intergenic 30.0%
! TTTACCTCTAAAAGTGTATC+AGG - chr1_2:14674738-14674757 None:intergenic 30.0%
!! GATACACTTTTAGAGGTAAA+TGG + chr1_2:14674738-14674757 Msa0058340:CDS 30.0%
! CCTTTGAAAAAACCACTATT+AGG - chr1_2:14674996-14675015 None:intergenic 30.0%
!!! CCTAATAGTGGTTTTTTCAA+AGG + chr1_2:14674993-14675012 Msa0058340:CDS 30.0%
GCAACATAAACTTCAATTTC+AGG - chr1_2:14675035-14675054 None:intergenic 30.0%
GAAATTGAAGTTTATGTTGC+TGG + chr1_2:14675035-14675054 Msa0058340:CDS 30.0%
AGTGTCCTAGTTAAGATTAT+AGG - chr1_2:14675077-14675096 None:intergenic 30.0%
ATTGCAGGAAGAAACATAAT+AGG + chr1_2:14675105-14675124 Msa0058340:CDS 30.0%
TATGAGTTTGATGAAATCAC+AGG + chr1_2:14675257-14675276 Msa0058340:intron 30.0%
ATGAGTTTGATGAAATCACA+GGG + chr1_2:14675258-14675277 Msa0058340:intron 30.0%
AATTTGCGAAATGTTCCATT+TGG + chr1_2:14675556-14675575 Msa0058340:intron 30.0%
AATGTTCCATTTGGTCTATA+AGG + chr1_2:14675565-14675584 Msa0058340:intron 30.0%
ATGTTCCATTTGGTCTATAA+GGG + chr1_2:14675566-14675585 Msa0058340:intron 30.0%
TATATCCCTTATAGACCAAA+TGG - chr1_2:14675574-14675593 None:intergenic 30.0%
! CTTTTACCATGCAATTTTTG+TGG + chr1_2:14675628-14675647 Msa0058340:intron 30.0%
! CACTTATAGGATAGGATTTT+TGG - chr1_2:14675869-14675888 None:intergenic 30.0%
AATCTATTAACAAGGGACAT+TGG - chr1_2:14675989-14676008 None:intergenic 30.0%
AGGTCAGAATCTATTAACAA+GGG - chr1_2:14675996-14676015 None:intergenic 30.0%
AAGGTCAGAATCTATTAACA+AGG - chr1_2:14675997-14676016 None:intergenic 30.0%
GACACAACATACAGAAAATA+AGG - chr1_2:14676054-14676073 None:intergenic 30.0%
! AAGGAGTAGATTGAAAGTTT+TGG - chr1_2:14676405-14676424 None:intergenic 30.0%
GGAAATAAACGTATTACTGA+AGG + chr1_2:14676471-14676490 Msa0058340:intron 30.0%
ATAAACAATATCTCCCCTAA+TGG + chr1_2:14676560-14676579 Msa0058340:intron 30.0%
GAGAAAAGGTAGTTATAAGA+AGG - chr1_2:14676595-14676614 None:intergenic 30.0%
TATAGTTACAATTGCTGATG+TGG - chr1_2:14676631-14676650 None:intergenic 30.0%
AGCAACCATCAAATACATAT+TGG + chr1_2:14676754-14676773 Msa0058340:intron 30.0%
TGGACCTTAGACAAATAAAT+GGG - chr1_2:14676794-14676813 None:intergenic 30.0%
TTGGACCTTAGACAAATAAA+TGG - chr1_2:14676795-14676814 None:intergenic 30.0%
!! ACTATGTTAGGTATTGGATT+TGG - chr1_2:14676814-14676833 None:intergenic 30.0%
CTAAAAGCAGATACTATGTT+AGG - chr1_2:14676826-14676845 None:intergenic 30.0%
CAAATTAAGTCCAAGTAACA+TGG - chr1_2:14676990-14677009 None:intergenic 30.0%
ACTTGGAAATATGTTGTTGA+AGG - chr1_2:14677026-14677045 None:intergenic 30.0%
!!! TTGTTTGGTTTTTGGTTCAT+AGG + chr1_2:14677100-14677119 Msa0058340:intron 30.0%
TATCTATATATCCTTCCCTT+TGG - chr1_2:14677184-14677203 None:intergenic 30.0%
AAGGGAAGGATATATAGATA+TGG + chr1_2:14677184-14677203 Msa0058340:CDS 30.0%
GGATATATAGATATGGAAGA+TGG + chr1_2:14677191-14677210 Msa0058340:CDS 30.0%
TCAATTACTTATCTACCACT+AGG + chr1_2:14677406-14677425 Msa0058340:three_prime_UTR 30.0%
!!! AGTTGCCCTTTTTTATATCT+AGG - chr1_2:14677488-14677507 None:intergenic 30.0%
GAAAGATTGTGTACTAAACA+AGG - chr1_2:14677538-14677557 None:intergenic 30.0%
AAGAAAGAGAACTTGAGTGA+AGG - chr1_2:14674287-14674306 None:intergenic 35.0%
TTCAGGCAAAATTACATCAG+CGG + chr1_2:14674393-14674412 Msa0058340:five_prime_UTR 35.0%
! TGTTTCGTTGATTGTGAAAC+AGG + chr1_2:14674425-14674444 Msa0058340:five_prime_UTR 35.0%
! ATTTTGGATATGAAGATGGG+AGG + chr1_2:14674504-14674523 Msa0058340:exon 35.0%
TATGATGCATCATCATGCAA+TGG + chr1_2:14674531-14674550 Msa0058340:CDS 35.0%
! AAGTCCTGATACACTTTTAG+AGG + chr1_2:14674731-14674750 Msa0058340:CDS 35.0%
! TAATAGGAAAGTGTGCATGT+TGG + chr1_2:14675121-14675140 Msa0058340:CDS 35.0%
GATGTTTGTGATCATGATCA+TGG + chr1_2:14675182-14675201 Msa0058340:CDS 35.0%
! TTGTTGAAGCATGTAGCTTT+TGG + chr1_2:14675292-14675311 Msa0058340:intron 35.0%
! AGCTTTTGGCAAAATCATGA+CGG + chr1_2:14675306-14675325 Msa0058340:intron 35.0%
AGTGCATGTTTGGATTAACA+GGG - chr1_2:14675536-14675555 None:intergenic 35.0%
TAGTGCATGTTTGGATTAAC+AGG - chr1_2:14675537-14675556 None:intergenic 35.0%
TTCGCAAATTAGTGCATGTT+TGG - chr1_2:14675546-14675565 None:intergenic 35.0%
! TTCGGATTTGGAATAAGATG+TGG + chr1_2:14675697-14675716 Msa0058340:intron 35.0%
TTGGGACCAATATCATCTAA+AGG - chr1_2:14675850-14675869 None:intergenic 35.0%
AACAGTCACACTTATAGGAT+AGG - chr1_2:14675877-14675896 None:intergenic 35.0%
TATAAGTGTGACTGTTGCAT+GGG + chr1_2:14675881-14675900 Msa0058340:intron 35.0%
ACCAGTACTTGTCCTTTAAT+GGG - chr1_2:14676021-14676040 None:intergenic 35.0%
ATGAAGCACAAACATAGACA+CGG + chr1_2:14676146-14676165 Msa0058340:intron 35.0%
! TGTAATCATAGGTATCGTGT+TGG + chr1_2:14676217-14676236 Msa0058340:intron 35.0%
! CTTCTAGTTGTACATTCATC+TGG - chr1_2:14676366-14676385 None:intergenic 35.0%
CAGATGAATGTACAACTAGA+AGG + chr1_2:14676364-14676383 Msa0058340:intron 35.0%
TTGAGTGTGATAGTAACCTA+AGG + chr1_2:14676450-14676469 Msa0058340:intron 35.0%
CTCTTTCTGTCTAAACCATA+AGG + chr1_2:14676522-14676541 Msa0058340:intron 35.0%
GTCAATATGCTTGAGAGAAA+AGG - chr1_2:14676609-14676628 None:intergenic 35.0%
! GTATTTGATGGTTGCTCAAT+AGG - chr1_2:14676750-14676769 None:intergenic 35.0%
GCAGATACTATGTTAGGTAT+TGG - chr1_2:14676820-14676839 None:intergenic 35.0%
GTTGTTGAAGGACTAATACT+AGG - chr1_2:14677014-14677033 None:intergenic 35.0%
TTGTAATGCTCTTCTTCTTC+TGG - chr1_2:14677149-14677168 None:intergenic 35.0%
ACAACAACATGTATTGCCAA+AGG + chr1_2:14677165-14677184 Msa0058340:CDS 35.0%
CAACAACATGTATTGCCAAA+GGG + chr1_2:14677166-14677185 Msa0058340:CDS 35.0%
!! ATCTTTGTAGGAGTTGGTAT+AGG + chr1_2:14677269-14677288 Msa0058340:CDS 35.0%
!! TCTTTGTAGGAGTTGGTATA+GGG + chr1_2:14677270-14677289 Msa0058340:CDS 35.0%
TGAAACTACGAGTAGTTCTT+TGG - chr1_2:14677319-14677338 None:intergenic 35.0%
CTACTTATCTGTGGAAAACT+TGG + chr1_2:14677506-14677525 Msa0058340:three_prime_UTR 35.0%
ACAACACTTCTGTATGAATG+TGG + chr1_2:14677649-14677668 Msa0058340:three_prime_UTR 35.0%
! ATGAATGTGGTTCTATAGCT+AGG + chr1_2:14677662-14677681 Msa0058340:three_prime_UTR 35.0%
AAAGAGAACTTGAGTGAAGG+TGG - chr1_2:14674284-14674303 None:intergenic 40.0%
ACAGAAATGGGACCTAATAG+TGG + chr1_2:14674981-14675000 Msa0058340:CDS 40.0%
! TGATCATGGACTTGTTTCAC+AGG + chr1_2:14675196-14675215 Msa0058340:CDS 40.0%
CATGCAACAGTCACACTTAT+AGG - chr1_2:14675882-14675901 None:intergenic 40.0%
CTATAAGTGTGACTGTTGCA+TGG + chr1_2:14675880-14675899 Msa0058340:intron 40.0%
ATTCTGACCTTCCCCATTAA+AGG + chr1_2:14676006-14676025 Msa0058340:intron 40.0%
TACTTGTCCTTTAATGGGGA+AGG - chr1_2:14676016-14676035 None:intergenic 40.0%
CCAGTACTTGTCCTTTAATG+GGG - chr1_2:14676020-14676039 None:intergenic 40.0%
CACCAGTACTTGTCCTTTAA+TGG - chr1_2:14676022-14676041 None:intergenic 40.0%
!! TAAAGGACAAGTACTGGTGT+TGG + chr1_2:14676023-14676042 Msa0058340:intron 40.0%
!! AAAGGACAAGTACTGGTGTT+GGG + chr1_2:14676024-14676043 Msa0058340:intron 40.0%
!! TATGAAGCACTGATACTCCT+CGG - chr1_2:14676285-14676304 None:intergenic 40.0%
AAGGAGACACTTACCATTAG+GGG - chr1_2:14676576-14676595 None:intergenic 40.0%
GAAGGAGACACTTACCATTA+GGG - chr1_2:14676577-14676596 None:intergenic 40.0%
AGAAGGAGACACTTACCATT+AGG - chr1_2:14676578-14676597 None:intergenic 40.0%
TGATCAGGAGCAAAATTGAG+GGG - chr1_2:14676677-14676696 None:intergenic 40.0%
CTGATCAGGAGCAAAATTGA+GGG - chr1_2:14676678-14676697 None:intergenic 40.0%
GGGCACCAATATGTATTTGA+TGG - chr1_2:14676762-14676781 None:intergenic 40.0%
AAATAAATGGGCCGGATAGA+GGG - chr1_2:14676782-14676801 None:intergenic 40.0%
CCTTAGACAAATAAATGGGC+CGG - chr1_2:14676790-14676809 None:intergenic 40.0%
TTGTGACACTCATAAGCACT+TGG - chr1_2:14677043-14677062 None:intergenic 40.0%
AACATGTATTGCCAAAGGGA+AGG + chr1_2:14677170-14677189 Msa0058340:CDS 40.0%
TTCAATGCTGGAGTAAGAGT+TGG + chr1_2:14677227-14677246 Msa0058340:CDS 40.0%
!! GTAAGAGTTGGTGTTGGAAT+TGG + chr1_2:14677239-14677258 Msa0058340:CDS 40.0%
ATTGGACTTGGCATCTTTGT+AGG + chr1_2:14677257-14677276 Msa0058340:CDS 40.0%
!! CTTTGTAGGAGTTGGTATAG+GGG + chr1_2:14677271-14677290 Msa0058340:CDS 40.0%
ACTACGAGTAGTTCTTTGGT+AGG - chr1_2:14677315-14677334 None:intergenic 40.0%
GAACTACTCGTAGTTTCAAG+AGG + chr1_2:14677321-14677340 Msa0058340:CDS 40.0%
AAAGGGCAACTACTTATCTG+TGG + chr1_2:14677497-14677516 Msa0058340:three_prime_UTR 40.0%
! TCATTTTCTGTGCCCTTCAA+AGG + chr1_2:14677556-14677575 Msa0058340:three_prime_UTR 40.0%
GATGCATCATCATGCAATGG+AGG + chr1_2:14674534-14674553 Msa0058340:CDS 45.0%
GCATGTTTGGATTAACAGGG+AGG - chr1_2:14675533-14675552 None:intergenic 45.0%
CCCCATTAAAGGACAAGTAC+TGG + chr1_2:14676017-14676036 Msa0058340:intron 45.0%
!! AAGGACAAGTACTGGTGTTG+GGG + chr1_2:14676025-14676044 Msa0058340:intron 45.0%
ACATAGACACGGACACAACA+CGG + chr1_2:14676157-14676176 Msa0058340:intron 45.0%
GATTAGGCGTGTCCTAGTAT+CGG - chr1_2:14676263-14676282 None:intergenic 45.0%
CCTGATCAGGAGCAAAATTG+AGG - chr1_2:14676679-14676698 None:intergenic 45.0%
! CCTCAATTTTGCTCCTGATC+AGG + chr1_2:14676676-14676695 Msa0058340:intron 45.0%
! ATCAGGTTGTAACTCCCGAT+CGG + chr1_2:14676693-14676712 Msa0058340:intron 45.0%
! ACATATTGGTGCCCTCTATC+CGG + chr1_2:14676768-14676787 Msa0058340:intron 45.0%
CAAATAAATGGGCCGGATAG+AGG - chr1_2:14676783-14676802 None:intergenic 45.0%
CCGGCCCATTTATTTGTCTA+AGG + chr1_2:14676787-14676806 Msa0058340:intron 45.0%
TGGAAGATGGTGAGCTATCA+TGG + chr1_2:14677204-14677223 Msa0058340:CDS 45.0%
GAGCTATCATGGTTCAATGC+TGG + chr1_2:14677215-14677234 Msa0058340:CDS 45.0%
!! GTTGGTGTTGGAATTGGACT+TGG + chr1_2:14677245-14677264 Msa0058340:CDS 45.0%
CTTGGCATCTTTGTAGGAGT+TGG + chr1_2:14677263-14677282 Msa0058340:CDS 45.0%
CTACTCGTAGTTTCAAGAGG+AGG + chr1_2:14677324-14677343 Msa0058340:CDS 45.0%
!!! ACTTATTTTTAAAAATTATT+AGG - chr1_2:14675361-14675380 None:intergenic 5.0%
!!! TAATAATTTTTAAAAATAAG+TGG + chr1_2:14675360-14675379 Msa0058340:intron 5.0%
!!! AATAATTTTTAAAAATAAGT+GGG + chr1_2:14675361-14675380 Msa0058340:intron 5.0%
GGACACTGCAGATCAATTGC+AGG + chr1_2:14675090-14675109 Msa0058340:CDS 50.0%
CACTGTGATTGCGTCAGACA+AGG + chr1_2:14675331-14675350 Msa0058340:intron 50.0%
! GCACTGATACTCCTCGGATT+AGG - chr1_2:14676279-14676298 None:intergenic 50.0%
TCGGGAGTTACAACCTGATC+AGG - chr1_2:14676692-14676711 None:intergenic 50.0%
!! GCTGGAGTAAGAGTTGGTGT+TGG + chr1_2:14677233-14677252 Msa0058340:CDS 50.0%
TTTGGTAGGAACGTGCCAAG+AGG - chr1_2:14677301-14677320 None:intergenic 50.0%
CTACCACTAGGTAGTGCGTA+CGG + chr1_2:14677418-14677437 Msa0058340:three_prime_UTR 50.0%
ATACCGTACGCACTACCTAG+TGG - chr1_2:14677424-14677443 None:intergenic 50.0%
ACTAGGTAGTGCGTACGGTA+TGG + chr1_2:14677423-14677442 Msa0058340:three_prime_UTR 50.0%
GTGATTGCGTCAGACAAGGC+CGG + chr1_2:14675335-14675354 Msa0058340:intron 55.0%
!! GTTTTGGCGGCCTTACCCTT+AGG + chr1_2:14675490-14675509 Msa0058340:intron 55.0%
AACAGGGAGGTCTGACGTCA+GGG - chr1_2:14675520-14675539 None:intergenic 55.0%
TAACAGGGAGGTCTGACGTC+AGG - chr1_2:14675521-14675540 None:intergenic 55.0%
AGTGTGACTGTTGCATGGGC+AGG + chr1_2:14675885-14675904 Msa0058340:intron 55.0%
!! GACAAGTACTGGTGTTGGGG+AGG + chr1_2:14676028-14676047 Msa0058340:intron 55.0%
! TCCTAGTATCGGACACGCGT+CGG - chr1_2:14676252-14676271 None:intergenic 55.0%
ACTAGGACACGCCTAATCCG+AGG + chr1_2:14676265-14676284 Msa0058340:intron 55.0%
TGGTATAGGGGTCAGCCTCT+TGG + chr1_2:14677283-14677302 Msa0058340:CDS 55.0%
TTGTTGTGGGGCCTAACGCG+AGG + chr1_2:14675467-14675486 Msa0058340:intron 60.0%
TGTTGTGGGGCCTAACGCGA+GGG + chr1_2:14675468-14675487 Msa0058340:intron 60.0%
!! GCCTAACGCGAGGGTTTTGG+CGG + chr1_2:14675477-14675496 Msa0058340:intron 60.0%
TCAGGGTCGGCCTAAGGGTA+AGG - chr1_2:14675503-14675522 None:intergenic 60.0%
TGACGTCAGGGTCGGCCTAA+GGG - chr1_2:14675508-14675527 None:intergenic 60.0%
ACCGACGCGTGTCCGATACT+AGG + chr1_2:14676248-14676267 Msa0058340:intron 60.0%
! GGGGCCTAACGCGAGGGTTT+TGG + chr1_2:14675474-14675493 Msa0058340:intron 65.0%
GCCGCCAAAACCCTCGCGTT+AGG - chr1_2:14675481-14675500 None:intergenic 65.0%
CTGACGTCAGGGTCGGCCTA+AGG - chr1_2:14675509-14675528 None:intergenic 65.0%
GGGAGGTCTGACGTCAGGGT+CGG - chr1_2:14675516-14675535 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 14674192 14677817 14674192 ID=Msa0058340;Name=Msa0058340
chr1_2 mRNA 14674192 14677817 14674192 ID=Msa0058340-mRNA-1;Parent=Msa0058340;Name=Msa0058340-mRNA-1;_AED=0.01;_eAED=0.01;_QI=321|1|1|1|1|1|2|462|312
chr1_2 exon 14674192 14675217 14674192 ID=Msa0058340-mRNA-1:exon:5888;Parent=Msa0058340-mRNA-1
chr1_2 exon 14677122 14677817 14677122 ID=Msa0058340-mRNA-1:exon:5889;Parent=Msa0058340-mRNA-1
chr1_2 five_prime_UTR 14674192 14674512 14674192 ID=Msa0058340-mRNA-1:five_prime_utr;Parent=Msa0058340-mRNA-1
chr1_2 CDS 14674513 14675217 14674513 ID=Msa0058340-mRNA-1:cds;Parent=Msa0058340-mRNA-1
chr1_2 CDS 14677122 14677355 14677122 ID=Msa0058340-mRNA-1:cds;Parent=Msa0058340-mRNA-1
chr1_2 three_prime_UTR 14677356 14677817 14677356 ID=Msa0058340-mRNA-1:three_prime_utr;Parent=Msa0058340-mRNA-1
Gene Sequence

>Msa0058340

ATGAAGATGGGAGGATCTTATGATGCATCATCATGCAATGGAGGAGAAGCAAGAAATAAAAGTCTTCAAATAATTGAATATTCACCTTATGATCCATCATGCATTAAATTATCATTTTCATTACCTTGGTTTGATGTAAGAGTTTTTTATGTAAGAGTTAGTGGAATTCAAGTGAATGAATCCACACCACAATTTCTATATCTTCATCATATACCTTTAAGTCCTGATACACTTTTAGAGGTAAATGGTGTTAGAAGTAACATGAATAGTGATGAAGATTCTTCAATTCTAAGAAGGGATAGAATTGATAAGAAATCTGAACAAGTCACTTTTGTGAATACAGATAGTATAAGAATAAATGGTAGCATGAAGTTTGAAGTTTGTGATAAAGATCAACGCATTCTCTCTTGTGTTCTTGAGATGAAAAGTGATGATAATGAGAAGAGATATTGGAGTATGAATTGTGAAACAGAAATGGGACCTAATAGTGGTTTTTTCAAAGGAAAACATGTTTCTGTTCCTGAAATTGAAGTTTATGTTGCTGGTTGCTTCTCAAGAAAACCTATAATCTTAACTAGGACACTGCAGATCAATTGCAGGAAGAAACATAATAGGAAAGTGTGCATGTTGGATGTGATTCCAGAATATGAAACATCTGAAATTAAGAAAGATGTTTGTGATCATGATCATGGACTTGTTTCACAGGTAGCTGAGTACAGAAGCTTCAAACCAGAAGAAGAAGAGCATTACAACAACATGTATTGCCAAAGGGAAGGATATATAGATATGGAAGATGGTGAGCTATCATGGTTCAATGCTGGAGTAAGAGTTGGTGTTGGAATTGGACTTGGCATCTTTGTAGGAGTTGGTATAGGGGTCAGCCTCTTGGCACGTTCCTACCAAAGAACTACTCGTAGTTTCAAGAGGAGGTTCATATAA
Protein sequence

>Msa0058340

MKMGGSYDASSCNGGEARNKSLQIIEYSPYDPSCIKLSFSLPWFDVRVFYVRVSGIQVNESTPQFLYLHHIPLSPDTLLEVNGVRSNMNSDEDSSILRRDRIDKKSEQVTFVNTDSIRINGSMKFEVCDKDQRILSCVLEMKSDDNEKRYWSMNCETEMGPNSGFFKGKHVSVPEIEVYVAGCFSRKPIILTRTLQINCRKKHNRKVCMLDVIPEYETSEIKKDVCDHDHGLVSQVAEYRSFKPEEEEHYNNMYCQREGYIDMEDGELSWFNAGVRVGVGIGLGIFVGVGIGVSLLARSYQRTTRSFKRRFI*