AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0146000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0146000 MtrunA17_Chr1g0152481 93.023 86 4 1 1 84 1 86 8.05e-30 101
Msa0146000 MtrunA17_Chr3g0134871 61.644 73 19 2 8 80 1 64 1.92e-12 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0146000 AT5G63905.1 68.493 73 23 0 11 83 3 75 3.13e-12 56.6

Find 43 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATCGGTGGTGAAAGAAAA+TGG 0.213474 1_4:-5728312 Msa0146000:three_prime_UTR
GGCCAATCGTTCTTCTTCTT+TGG 0.267606 1_4:+5729834 None:intergenic
GAGTTTCTGTAGAACGAAAA+TGG 0.308370 1_4:+5728223 None:intergenic
GTTGACTGATTCCAGCTTTG+AGG 0.313445 1_4:-5728187 Msa0146000:three_prime_UTR
TTGTCCCGAAGATGATTTAC+AGG 0.328544 1_4:-5728409 Msa0146000:three_prime_UTR
AATCGATCACTATGAGATAT+TGG 0.346724 1_4:-5728095 Msa0146000:three_prime_UTR
TTGACTGATTCCAGCTTTGA+GGG 0.362303 1_4:-5728186 Msa0146000:three_prime_UTR
AATAAATATATCTGTTTGTA+AGG 0.388436 1_4:-5728870 Msa0146000:intron
AAGTTCCTGTAAATCATCTT+CGG 0.390419 1_4:+5728404 None:intergenic
GCTGCACGTGCACATCAAAA+AGG 0.391519 1_4:-5729149 Msa0146000:CDS
AAGAAGATGGGTATATGCTT+TGG 0.393509 1_4:-5729886 Msa0146000:CDS
CAAGCTGCAAACTCAAATAA+AGG 0.408424 1_4:-5729116 Msa0146000:CDS
CTATGTAAGGCCCTCAAAGC+TGG 0.418267 1_4:+5728176 None:intergenic
CTGCTGAAGCCGCTCAGAAA+AGG 0.423944 1_4:-5729789 Msa0146000:intron
AGTTCCTGTAAATCATCTTC+GGG 0.445174 1_4:+5728405 None:intergenic
CAAAGCAAATCAAGCAAAGT+TGG 0.445816 1_4:+5728131 None:intergenic
GGTATATGCTTTGGTTGCTT+CGG 0.447661 1_4:-5729877 Msa0146000:CDS
ATGTTACATGTAACTATGTA+AGG 0.448764 1_4:+5728163 None:intergenic
CGATATATATAAAGAGAAAG+AGG 0.453670 1_4:-5729932 Msa0146000:five_prime_UTR
CAAAGCTGGAATCAGTCAAC+AGG 0.463500 1_4:+5728190 None:intergenic
AATCGGTGGTGAAAGAAAAT+GGG 0.464135 1_4:-5728311 Msa0146000:three_prime_UTR
GAACGAAGGATTAAAGAAGA+TGG 0.473381 1_4:-5729899 Msa0146000:CDS
GTGGCAGATGGGCTGAGGTT+AGG 0.489574 1_4:-5728894 Msa0146000:exon
TCTGTGGATGCAGTGGCAGA+TGG 0.491765 1_4:-5728906 Msa0146000:intron
TCTGTTTGTTTGTAAAGAAT+CGG 0.496098 1_4:-5728328 Msa0146000:three_prime_UTR
AACGAAGGATTAAAGAAGAT+GGG 0.507717 1_4:-5729898 Msa0146000:CDS
GATATATATAAAGAGAAAGA+GGG 0.508182 1_4:-5729931 Msa0146000:five_prime_UTR
ATATGCTTTGGTTGCTTCGG+TGG 0.510299 1_4:-5729874 Msa0146000:CDS
TGCTTTGGTTGCTTCGGTGG+CGG 0.510377 1_4:-5729871 Msa0146000:CDS
TTGTGTTGATTGCAGGCAAA+AGG 0.512011 1_4:-5729198 Msa0146000:intron
TAAAGGCGAACCAACTCTAA+AGG 0.521986 1_4:-5729099 Msa0146000:intron
GACTTTGAGAAATCTGCAGC+AGG 0.532357 1_4:-5729176 Msa0146000:CDS
GACCAAAGAAGAAGAACGAT+TGG 0.539638 1_4:-5729836 Msa0146000:CDS
TGTAAATCATCTTCGGGACA+AGG 0.544700 1_4:+5728411 None:intergenic
AGTCACCAAACCTTTAGAGT+TGG 0.547268 1_4:+5729089 None:intergenic
AAGCACGCTGTGTATAGAAT+CGG 0.550284 1_4:+5728435 None:intergenic
CGCTGTGTATAGAATCGGAC+TGG 0.551942 1_4:+5728440 None:intergenic
CTGTGGATGCAGTGGCAGAT+GGG 0.552408 1_4:-5728905 Msa0146000:intron
TCTAGCACGCGCTTCTTCAG+AGG 0.566908 1_4:+5729813 None:intergenic
AAAATGGGTATATGTTGCGA+AGG 0.595310 1_4:-5728296 Msa0146000:three_prime_UTR
ATGCAGTGGCAGATGGGCTG+AGG 0.609439 1_4:-5728899 Msa0146000:intron
GTAAATCATCTTCGGGACAA+GGG 0.622918 1_4:+5728412 None:intergenic
GTTTGTTTGTAAAGAATCGG+TGG 0.632432 1_4:-5728325 Msa0146000:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAACTTTGT+AGG + chr1_4:5728347-5728366 None:intergenic 10.0%
!!! TTGTATTAAAAAATGAATTT+TGG - chr1_4:5728445-5728464 Msa0146000:three_prime_UTR 10.0%
!! TTAAATTCATTACATATTTA+GGG + chr1_4:5728693-5728712 None:intergenic 10.0%
!! TGATTTATGTTAATATAAAA+AGG - chr1_4:5729511-5729530 Msa0146000:intron 10.0%
!! AAAAAAAAAAAAAACTTTGT+AGG + chr1_4:5728347-5728366 None:intergenic 10.0%
!!! TTGTATTAAAAAATGAATTT+TGG - chr1_4:5728445-5728464 Msa0146000:three_prime_UTR 10.0%
!! TTAAATTCATTACATATTTA+GGG + chr1_4:5728693-5728712 None:intergenic 10.0%
!! TGATTTATGTTAATATAAAA+AGG - chr1_4:5729511-5729530 Msa0146000:intron 10.0%
!!! TTTTTTTTTTTTAACGGATT+TGG - chr1_4:5728358-5728377 Msa0146000:three_prime_UTR 15.0%
!!! TTTTTTTTTTTAACGGATTT+GGG - chr1_4:5728359-5728378 Msa0146000:three_prime_UTR 15.0%
!! CTTAAATTCATTACATATTT+AGG + chr1_4:5728694-5728713 None:intergenic 15.0%
!! AAAATAAATGATAACAAAAG+TGG + chr1_4:5729004-5729023 None:intergenic 15.0%
!! AATAAATATATCTGTTTGTA+AGG - chr1_4:5729168-5729187 Msa0146000:CDS 15.0%
!!! AATTTAGGACTAAATTAATT+TGG - chr1_4:5729379-5729398 Msa0146000:intron 15.0%
!!! TTTTTTTTTTTTAACGGATT+TGG - chr1_4:5728358-5728377 Msa0146000:three_prime_UTR 15.0%
!!! TTTTTTTTTTTAACGGATTT+GGG - chr1_4:5728359-5728378 Msa0146000:three_prime_UTR 15.0%
!! CTTAAATTCATTACATATTT+AGG + chr1_4:5728694-5728713 None:intergenic 15.0%
!! AAAATAAATGATAACAAAAG+TGG + chr1_4:5729004-5729023 None:intergenic 15.0%
!! AATAAATATATCTGTTTGTA+AGG - chr1_4:5729168-5729187 Msa0146000:CDS 15.0%
!!! AATTTAGGACTAAATTAATT+TGG - chr1_4:5729379-5729398 Msa0146000:intron 15.0%
!! GATATATATAAAGAGAAAGA+GGG - chr1_4:5728107-5728126 Msa0146000:three_prime_UTR 20.0%
!!! TTTTCATTTCAATACTCATA+TGG - chr1_4:5728383-5728402 Msa0146000:three_prime_UTR 20.0%
!! TTTCATTTCAATACTCATAT+GGG - chr1_4:5728384-5728403 Msa0146000:three_prime_UTR 20.0%
!! TAAATGTATCTGAAAATTGT+TGG + chr1_4:5729080-5729099 None:intergenic 20.0%
!! GATATATATAAAGAGAAAGA+GGG - chr1_4:5728107-5728126 Msa0146000:three_prime_UTR 20.0%
!!! TTTTCATTTCAATACTCATA+TGG - chr1_4:5728383-5728402 Msa0146000:three_prime_UTR 20.0%
!! TTTCATTTCAATACTCATAT+GGG - chr1_4:5728384-5728403 Msa0146000:three_prime_UTR 20.0%
!! TAAATGTATCTGAAAATTGT+TGG + chr1_4:5729080-5729099 None:intergenic 20.0%
! CGATATATATAAAGAGAAAG+AGG - chr1_4:5728106-5728125 Msa0146000:three_prime_UTR 25.0%
! ATTCAGGAGAAAAAAGAAAA+GGG + chr1_4:5728298-5728317 None:intergenic 25.0%
! TATTCAGGAGAAAAAAGAAA+AGG + chr1_4:5728299-5728318 None:intergenic 25.0%
! AATTGTACAATTAAGTTTGG+TGG + chr1_4:5728324-5728343 None:intergenic 25.0%
! AGGAATTGTACAATTAAGTT+TGG + chr1_4:5728327-5728346 None:intergenic 25.0%
! TATATTCTTTGTGATCATAC+TGG - chr1_4:5728411-5728430 Msa0146000:three_prime_UTR 25.0%
! ATAGAAACAACTTATGAGAT+GGG + chr1_4:5728601-5728620 None:intergenic 25.0%
! TATAGAAACAACTTATGAGA+TGG + chr1_4:5728602-5728621 None:intergenic 25.0%
!!! ATGTTATGATGTTATTTTGC+TGG - chr1_4:5728766-5728785 Msa0146000:intron 25.0%
! AATTGTTGGTCAAAAAACAT+GGG + chr1_4:5729066-5729085 None:intergenic 25.0%
! AAATTGTTGGTCAAAAAACA+TGG + chr1_4:5729067-5729086 None:intergenic 25.0%
! TTATAGTTATCCTGTTCATT+CGG - chr1_4:5729211-5729230 Msa0146000:intron 25.0%
! GAATTCAATCTAAATTCAAC+TGG + chr1_4:5729436-5729455 None:intergenic 25.0%
!!! TTTTTAAAGCAGTGTAGATT+AGG - chr1_4:5729462-5729481 Msa0146000:intron 25.0%
! TCTGTTTGTTTGTAAAGAAT+CGG - chr1_4:5729710-5729729 Msa0146000:intron 25.0%
! ATGTTACATGTAACTATGTA+AGG + chr1_4:5729878-5729897 None:intergenic 25.0%
! CGATATATATAAAGAGAAAG+AGG - chr1_4:5728106-5728125 Msa0146000:three_prime_UTR 25.0%
! ATTCAGGAGAAAAAAGAAAA+GGG + chr1_4:5728298-5728317 None:intergenic 25.0%
! TATTCAGGAGAAAAAAGAAA+AGG + chr1_4:5728299-5728318 None:intergenic 25.0%
! AATTGTACAATTAAGTTTGG+TGG + chr1_4:5728324-5728343 None:intergenic 25.0%
! AGGAATTGTACAATTAAGTT+TGG + chr1_4:5728327-5728346 None:intergenic 25.0%
! TATATTCTTTGTGATCATAC+TGG - chr1_4:5728411-5728430 Msa0146000:three_prime_UTR 25.0%
! ATAGAAACAACTTATGAGAT+GGG + chr1_4:5728601-5728620 None:intergenic 25.0%
! TATAGAAACAACTTATGAGA+TGG + chr1_4:5728602-5728621 None:intergenic 25.0%
!!! ATGTTATGATGTTATTTTGC+TGG - chr1_4:5728766-5728785 Msa0146000:intron 25.0%
! AATTGTTGGTCAAAAAACAT+GGG + chr1_4:5729066-5729085 None:intergenic 25.0%
! AAATTGTTGGTCAAAAAACA+TGG + chr1_4:5729067-5729086 None:intergenic 25.0%
! TTATAGTTATCCTGTTCATT+CGG - chr1_4:5729211-5729230 Msa0146000:intron 25.0%
! GAATTCAATCTAAATTCAAC+TGG + chr1_4:5729436-5729455 None:intergenic 25.0%
!!! TTTTTAAAGCAGTGTAGATT+AGG - chr1_4:5729462-5729481 Msa0146000:intron 25.0%
! TCTGTTTGTTTGTAAAGAAT+CGG - chr1_4:5729710-5729729 Msa0146000:intron 25.0%
! ATGTTACATGTAACTATGTA+AGG + chr1_4:5729878-5729897 None:intergenic 25.0%
AACGAAGGATTAAAGAAGAT+GGG - chr1_4:5728140-5728159 Msa0146000:three_prime_UTR 30.0%
AAAGGGGAATTTAGAGAAAA+AGG + chr1_4:5728281-5728300 None:intergenic 30.0%
TTCAGGAGAAAAAAGAAAAG+GGG + chr1_4:5728297-5728316 None:intergenic 30.0%
! TTAAGTTTGGTGGAATATTC+AGG + chr1_4:5728314-5728333 None:intergenic 30.0%
ACTAGAGATAAGCTCAAATA+AGG - chr1_4:5728562-5728581 Msa0146000:intron 30.0%
CTCAAATAAGGCAATTCAAA+CGG - chr1_4:5728574-5728593 Msa0146000:intron 30.0%
TCAAATAAGGCAATTCAAAC+GGG - chr1_4:5728575-5728594 Msa0146000:intron 30.0%
AAACACTTGTGAAACTGTTT+GGG + chr1_4:5728631-5728650 None:intergenic 30.0%
TAAACACTTGTGAAACTGTT+TGG + chr1_4:5728632-5728651 None:intergenic 30.0%
! TTACATATTTAGGGTGTGTT+TGG + chr1_4:5728684-5728703 None:intergenic 30.0%
GAATTTAAGCTTACATGTTG+AGG - chr1_4:5728705-5728724 Msa0146000:intron 30.0%
GTAGAAACTCTTAATAAAGG+TGG + chr1_4:5728982-5729001 None:intergenic 30.0%
GTGGTAGAAACTCTTAATAA+AGG + chr1_4:5728985-5729004 None:intergenic 30.0%
!!! CGTTTTGATTCTTTTATCTG+TGG - chr1_4:5729116-5729135 Msa0146000:CDS 30.0%
GAGTTTGCACTCAAAAATTT+AGG - chr1_4:5729364-5729383 Msa0146000:intron 30.0%
!! TCTAAATTCAACTGGTTTGT+TGG + chr1_4:5729428-5729447 None:intergenic 30.0%
! TGTTTTACAGTATCAATGAC+AGG - chr1_4:5729574-5729593 Msa0146000:intron 30.0%
AAGTTCCTGTAAATCATCTT+CGG + chr1_4:5729637-5729656 None:intergenic 30.0%
! ATTTTCGTTCTACAGAAACT+CGG - chr1_4:5729817-5729836 Msa0146000:CDS 30.0%
AATCGATCACTATGAGATAT+TGG - chr1_4:5729943-5729962 Msa0146000:five_prime_UTR 30.0%
AACGAAGGATTAAAGAAGAT+GGG - chr1_4:5728140-5728159 Msa0146000:three_prime_UTR 30.0%
AAAGGGGAATTTAGAGAAAA+AGG + chr1_4:5728281-5728300 None:intergenic 30.0%
TTCAGGAGAAAAAAGAAAAG+GGG + chr1_4:5728297-5728316 None:intergenic 30.0%
! TTAAGTTTGGTGGAATATTC+AGG + chr1_4:5728314-5728333 None:intergenic 30.0%
ACTAGAGATAAGCTCAAATA+AGG - chr1_4:5728562-5728581 Msa0146000:intron 30.0%
CTCAAATAAGGCAATTCAAA+CGG - chr1_4:5728574-5728593 Msa0146000:intron 30.0%
TCAAATAAGGCAATTCAAAC+GGG - chr1_4:5728575-5728594 Msa0146000:intron 30.0%
AAACACTTGTGAAACTGTTT+GGG + chr1_4:5728631-5728650 None:intergenic 30.0%
TAAACACTTGTGAAACTGTT+TGG + chr1_4:5728632-5728651 None:intergenic 30.0%
! TTACATATTTAGGGTGTGTT+TGG + chr1_4:5728684-5728703 None:intergenic 30.0%
GAATTTAAGCTTACATGTTG+AGG - chr1_4:5728705-5728724 Msa0146000:intron 30.0%
GTAGAAACTCTTAATAAAGG+TGG + chr1_4:5728982-5729001 None:intergenic 30.0%
GTGGTAGAAACTCTTAATAA+AGG + chr1_4:5728985-5729004 None:intergenic 30.0%
!!! CGTTTTGATTCTTTTATCTG+TGG - chr1_4:5729116-5729135 Msa0146000:CDS 30.0%
GAGTTTGCACTCAAAAATTT+AGG - chr1_4:5729364-5729383 Msa0146000:intron 30.0%
!! TCTAAATTCAACTGGTTTGT+TGG + chr1_4:5729428-5729447 None:intergenic 30.0%
! TGTTTTACAGTATCAATGAC+AGG - chr1_4:5729574-5729593 Msa0146000:intron 30.0%
AAGTTCCTGTAAATCATCTT+CGG + chr1_4:5729637-5729656 None:intergenic 30.0%
! ATTTTCGTTCTACAGAAACT+CGG - chr1_4:5729817-5729836 Msa0146000:CDS 30.0%
GAACGAAGGATTAAAGAAGA+TGG - chr1_4:5728139-5728158 Msa0146000:three_prime_UTR 35.0%
AAGAAGATGGGTATATGCTT+TGG - chr1_4:5728152-5728171 Msa0146000:three_prime_UTR 35.0%
! AGGTTTAAACCTTTTCTGAG+CGG + chr1_4:5728261-5728280 None:intergenic 35.0%
TTTAAGCTTACATGTTGAGG+TGG - chr1_4:5728708-5728727 Msa0146000:intron 35.0%
TTAAGCTTACATGTTGAGGT+GGG - chr1_4:5728709-5728728 Msa0146000:intron 35.0%
CAAGCTGCAAACTCAAATAA+AGG - chr1_4:5728922-5728941 Msa0146000:intron 35.0%
AAAGAAAGCAGCATATTGCA+AGG + chr1_4:5729039-5729058 None:intergenic 35.0%
GTGCAAACTCAAAACAAACA+AGG + chr1_4:5729354-5729373 None:intergenic 35.0%
! AGTAGTGCTTTCGATTTGAT+TGG - chr1_4:5729541-5729560 Msa0146000:intron 35.0%
AGTTCCTGTAAATCATCTTC+GGG + chr1_4:5729636-5729655 None:intergenic 35.0%
GTTTGTTTGTAAAGAATCGG+TGG - chr1_4:5729713-5729732 Msa0146000:intron 35.0%
AATCGGTGGTGAAAGAAAAT+GGG - chr1_4:5729727-5729746 Msa0146000:intron 35.0%
AAAATGGGTATATGTTGCGA+AGG - chr1_4:5729742-5729761 Msa0146000:intron 35.0%
GAGTTTCTGTAGAACGAAAA+TGG + chr1_4:5729818-5729837 None:intergenic 35.0%
CAAAGCAAATCAAGCAAAGT+TGG + chr1_4:5729910-5729929 None:intergenic 35.0%
GAACGAAGGATTAAAGAAGA+TGG - chr1_4:5728139-5728158 Msa0146000:three_prime_UTR 35.0%
AAGAAGATGGGTATATGCTT+TGG - chr1_4:5728152-5728171 Msa0146000:three_prime_UTR 35.0%
! AGGTTTAAACCTTTTCTGAG+CGG + chr1_4:5728261-5728280 None:intergenic 35.0%
TTTAAGCTTACATGTTGAGG+TGG - chr1_4:5728708-5728727 Msa0146000:intron 35.0%
TTAAGCTTACATGTTGAGGT+GGG - chr1_4:5728709-5728728 Msa0146000:intron 35.0%
CAAGCTGCAAACTCAAATAA+AGG - chr1_4:5728922-5728941 Msa0146000:intron 35.0%
AAAGAAAGCAGCATATTGCA+AGG + chr1_4:5729039-5729058 None:intergenic 35.0%
GTGCAAACTCAAAACAAACA+AGG + chr1_4:5729354-5729373 None:intergenic 35.0%
! AGTAGTGCTTTCGATTTGAT+TGG - chr1_4:5729541-5729560 Msa0146000:intron 35.0%
AGTTCCTGTAAATCATCTTC+GGG + chr1_4:5729636-5729655 None:intergenic 35.0%
GTTTGTTTGTAAAGAATCGG+TGG - chr1_4:5729713-5729732 Msa0146000:intron 35.0%
AATCGGTGGTGAAAGAAAAT+GGG - chr1_4:5729727-5729746 Msa0146000:intron 35.0%
AAAATGGGTATATGTTGCGA+AGG - chr1_4:5729742-5729761 Msa0146000:intron 35.0%
GAGTTTCTGTAGAACGAAAA+TGG + chr1_4:5729818-5729837 None:intergenic 35.0%
CAAAGCAAATCAAGCAAAGT+TGG + chr1_4:5729910-5729929 None:intergenic 35.0%
GAGGGAGAAAAAATGAACGA+AGG - chr1_4:5728125-5728144 Msa0146000:three_prime_UTR 40.0%
GGTATATGCTTTGGTTGCTT+CGG - chr1_4:5728161-5728180 Msa0146000:three_prime_UTR 40.0%
GACCAAAGAAGAAGAACGAT+TGG - chr1_4:5728202-5728221 Msa0146000:three_prime_UTR 40.0%
TAAGCTTACATGTTGAGGTG+GGG - chr1_4:5728710-5728729 Msa0146000:intron 40.0%
!! GTATGTGTTGTGTTGATTGC+AGG - chr1_4:5728833-5728852 Msa0146000:intron 40.0%
!! TTGTGTTGATTGCAGGCAAA+AGG - chr1_4:5728840-5728859 Msa0146000:intron 40.0%
ACGTGCACATCAAAAAGGAA+TGG - chr1_4:5728894-5728913 Msa0146000:exon 40.0%
TAAAGGCGAACCAACTCTAA+AGG - chr1_4:5728939-5728958 Msa0146000:intron 40.0%
!! AGTCACCAAACCTTTAGAGT+TGG + chr1_4:5728952-5728971 None:intergenic 40.0%
! TCTTTTATCTGTGGATGCAG+TGG - chr1_4:5729125-5729144 Msa0146000:CDS 40.0%
AGTTGCATAACCGAATGAAC+AGG + chr1_4:5729224-5729243 None:intergenic 40.0%
CAAACAAGGATATGCTCACT+TGG + chr1_4:5729340-5729359 None:intergenic 40.0%
AAGCACGCTGTGTATAGAAT+CGG + chr1_4:5729606-5729625 None:intergenic 40.0%
GTAAATCATCTTCGGGACAA+GGG + chr1_4:5729629-5729648 None:intergenic 40.0%
TGTAAATCATCTTCGGGACA+AGG + chr1_4:5729630-5729649 None:intergenic 40.0%
TTGTCCCGAAGATGATTTAC+AGG - chr1_4:5729629-5729648 Msa0146000:intron 40.0%
GAATCGGTGGTGAAAGAAAA+TGG - chr1_4:5729726-5729745 Msa0146000:intron 40.0%
! TTGACTGATTCCAGCTTTGA+GGG - chr1_4:5729852-5729871 Msa0146000:CDS 40.0%
GAGGGAGAAAAAATGAACGA+AGG - chr1_4:5728125-5728144 Msa0146000:three_prime_UTR 40.0%
GGTATATGCTTTGGTTGCTT+CGG - chr1_4:5728161-5728180 Msa0146000:three_prime_UTR 40.0%
GACCAAAGAAGAAGAACGAT+TGG - chr1_4:5728202-5728221 Msa0146000:three_prime_UTR 40.0%
TAAGCTTACATGTTGAGGTG+GGG - chr1_4:5728710-5728729 Msa0146000:intron 40.0%
!! GTATGTGTTGTGTTGATTGC+AGG - chr1_4:5728833-5728852 Msa0146000:intron 40.0%
!! TTGTGTTGATTGCAGGCAAA+AGG - chr1_4:5728840-5728859 Msa0146000:intron 40.0%
ACGTGCACATCAAAAAGGAA+TGG - chr1_4:5728894-5728913 Msa0146000:exon 40.0%
TAAAGGCGAACCAACTCTAA+AGG - chr1_4:5728939-5728958 Msa0146000:intron 40.0%
!! AGTCACCAAACCTTTAGAGT+TGG + chr1_4:5728952-5728971 None:intergenic 40.0%
! TCTTTTATCTGTGGATGCAG+TGG - chr1_4:5729125-5729144 Msa0146000:CDS 40.0%
AGTTGCATAACCGAATGAAC+AGG + chr1_4:5729224-5729243 None:intergenic 40.0%
CAAACAAGGATATGCTCACT+TGG + chr1_4:5729340-5729359 None:intergenic 40.0%
AAGCACGCTGTGTATAGAAT+CGG + chr1_4:5729606-5729625 None:intergenic 40.0%
GTAAATCATCTTCGGGACAA+GGG + chr1_4:5729629-5729648 None:intergenic 40.0%
TGTAAATCATCTTCGGGACA+AGG + chr1_4:5729630-5729649 None:intergenic 40.0%
TTGTCCCGAAGATGATTTAC+AGG - chr1_4:5729629-5729648 Msa0146000:intron 40.0%
GAATCGGTGGTGAAAGAAAA+TGG - chr1_4:5729726-5729745 Msa0146000:intron 40.0%
! TTGACTGATTCCAGCTTTGA+GGG - chr1_4:5729852-5729871 Msa0146000:CDS 40.0%
ATATGCTTTGGTTGCTTCGG+TGG - chr1_4:5728164-5728183 Msa0146000:three_prime_UTR 45.0%
GGCCAATCGTTCTTCTTCTT+TGG + chr1_4:5728207-5728226 None:intergenic 45.0%
AAGCTTACATGTTGAGGTGG+GGG - chr1_4:5728711-5728730 Msa0146000:intron 45.0%
! GACTTTGAGAAATCTGCAGC+AGG - chr1_4:5728862-5728881 Msa0146000:intron 45.0%
! GCGAACCAACTCTAAAGGTT+TGG - chr1_4:5728944-5728963 Msa0146000:intron 45.0%
ATCGAGGTGCGCAATACAAT+AGG + chr1_4:5729311-5729330 None:intergenic 45.0%
GCAGTGTAGATTAGGATCAG+TGG - chr1_4:5729470-5729489 Msa0146000:intron 45.0%
CAAAGCTGGAATCAGTCAAC+AGG + chr1_4:5729851-5729870 None:intergenic 45.0%
! GTTGACTGATTCCAGCTTTG+AGG - chr1_4:5729851-5729870 Msa0146000:CDS 45.0%
ATATGCTTTGGTTGCTTCGG+TGG - chr1_4:5728164-5728183 Msa0146000:three_prime_UTR 45.0%
GGCCAATCGTTCTTCTTCTT+TGG + chr1_4:5728207-5728226 None:intergenic 45.0%
AAGCTTACATGTTGAGGTGG+GGG - chr1_4:5728711-5728730 Msa0146000:intron 45.0%
! GACTTTGAGAAATCTGCAGC+AGG - chr1_4:5728862-5728881 Msa0146000:intron 45.0%
! GCGAACCAACTCTAAAGGTT+TGG - chr1_4:5728944-5728963 Msa0146000:intron 45.0%
ATCGAGGTGCGCAATACAAT+AGG + chr1_4:5729311-5729330 None:intergenic 45.0%
GCAGTGTAGATTAGGATCAG+TGG - chr1_4:5729470-5729489 Msa0146000:intron 45.0%
CAAAGCTGGAATCAGTCAAC+AGG + chr1_4:5729851-5729870 None:intergenic 45.0%
! GTTGACTGATTCCAGCTTTG+AGG - chr1_4:5729851-5729870 Msa0146000:CDS 45.0%
!!! GTTTTTTTTTTTTTTTTTAA+CGG - chr1_4:5728352-5728371 Msa0146000:three_prime_UTR 5.0%
!!! GTTTTTTTTTTTTTTTTTAA+CGG - chr1_4:5728352-5728371 Msa0146000:three_prime_UTR 5.0%
GCTGCACGTGCACATCAAAA+AGG - chr1_4:5728889-5728908 Msa0146000:exon 50.0%
! GCTCACTTGGAAGTACATCG+AGG + chr1_4:5729327-5729346 None:intergenic 50.0%
CGCTGTGTATAGAATCGGAC+TGG + chr1_4:5729601-5729620 None:intergenic 50.0%
CTATGTAAGGCCCTCAAAGC+TGG + chr1_4:5729865-5729884 None:intergenic 50.0%
GCTGCACGTGCACATCAAAA+AGG - chr1_4:5728889-5728908 Msa0146000:exon 50.0%
! GCTCACTTGGAAGTACATCG+AGG + chr1_4:5729327-5729346 None:intergenic 50.0%
CGCTGTGTATAGAATCGGAC+TGG + chr1_4:5729601-5729620 None:intergenic 50.0%
CTATGTAAGGCCCTCAAAGC+TGG + chr1_4:5729865-5729884 None:intergenic 50.0%
TGCTTTGGTTGCTTCGGTGG+CGG - chr1_4:5728167-5728186 Msa0146000:three_prime_UTR 55.0%
TCTAGCACGCGCTTCTTCAG+AGG + chr1_4:5728228-5728247 None:intergenic 55.0%
CTGCTGAAGCCGCTCAGAAA+AGG - chr1_4:5728249-5728268 Msa0146000:three_prime_UTR 55.0%
CTTACATGTTGAGGTGGGGG+TGG - chr1_4:5728714-5728733 Msa0146000:intron 55.0%
TCTGTGGATGCAGTGGCAGA+TGG - chr1_4:5729132-5729151 Msa0146000:CDS 55.0%
CTGTGGATGCAGTGGCAGAT+GGG - chr1_4:5729133-5729152 Msa0146000:CDS 55.0%
TGCTTTGGTTGCTTCGGTGG+CGG - chr1_4:5728167-5728186 Msa0146000:three_prime_UTR 55.0%
TCTAGCACGCGCTTCTTCAG+AGG + chr1_4:5728228-5728247 None:intergenic 55.0%
CTGCTGAAGCCGCTCAGAAA+AGG - chr1_4:5728249-5728268 Msa0146000:three_prime_UTR 55.0%
CTTACATGTTGAGGTGGGGG+TGG - chr1_4:5728714-5728733 Msa0146000:intron 55.0%
TCTGTGGATGCAGTGGCAGA+TGG - chr1_4:5729132-5729151 Msa0146000:CDS 55.0%
CTGTGGATGCAGTGGCAGAT+GGG - chr1_4:5729133-5729152 Msa0146000:CDS 55.0%
!! ATGCAGTGGCAGATGGGCTG+AGG - chr1_4:5729139-5729158 Msa0146000:CDS 60.0%
!! GTGGCAGATGGGCTGAGGTT+AGG - chr1_4:5729144-5729163 Msa0146000:CDS 60.0%
!! ATGCAGTGGCAGATGGGCTG+AGG - chr1_4:5729139-5729158 Msa0146000:CDS 60.0%
!! GTGGCAGATGGGCTGAGGTT+AGG - chr1_4:5729144-5729163 Msa0146000:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 5728095 5729965 5728095 ID=Msa0146000;Name=Msa0146000
chr1_4 mRNA 5728095 5729965 5728095 ID=Msa0146000-mRNA-1;Parent=Msa0146000;Name=Msa0146000-mRNA-1;_AED=0.00;_eAED=0.00;_QI=42|1|1|1|0.66|0.5|4|412|84
chr1_4 exon 5728095 5728476 5728095 ID=Msa0146000-mRNA-1:exon:2435;Parent=Msa0146000-mRNA-1
chr1_4 exon 5728871 5728915 5728871 ID=Msa0146000-mRNA-1:exon:2434;Parent=Msa0146000-mRNA-1
chr1_4 exon 5729100 5729205 5729100 ID=Msa0146000-mRNA-1:exon:2433;Parent=Msa0146000-mRNA-1
chr1_4 exon 5729790 5729965 5729790 ID=Msa0146000-mRNA-1:exon:2432;Parent=Msa0146000-mRNA-1
chr1_4 five_prime_UTR 5729924 5729965 5729924 ID=Msa0146000-mRNA-1:five_prime_utr;Parent=Msa0146000-mRNA-1
chr1_4 CDS 5729790 5729923 5729790 ID=Msa0146000-mRNA-1:cds;Parent=Msa0146000-mRNA-1
chr1_4 CDS 5729100 5729205 5729100 ID=Msa0146000-mRNA-1:cds;Parent=Msa0146000-mRNA-1
chr1_4 CDS 5728901 5728915 5728901 ID=Msa0146000-mRNA-1:cds;Parent=Msa0146000-mRNA-1
chr1_4 three_prime_UTR 5728871 5728900 5728871 ID=Msa0146000-mRNA-1:three_prime_utr;Parent=Msa0146000-mRNA-1
chr1_4 three_prime_UTR 5728095 5728476 5728095 ID=Msa0146000-mRNA-1:three_prime_utr;Parent=Msa0146000-mRNA-1
Gene Sequence

>Msa0146000

ATGAACGAAGGATTAAAGAAGATGGGTATATGCTTTGGTTGCTTCGGTGGCGGTGACAAGCGCATGACCAAAGAAGAAGAACGATTGGCCTCTGAAGAAGCGCGTGCTAGAGCTGCTGAAGCCGCTCAGAAAAGGCAAAAGGACTTTGAGAAATCTGCAGCAGGACGAGCTGCACGTGCACATCAAAAAGGAATGGCAAAGCAAGCTGCAAACTCAAATAAAGGCGAACCAACTCTAAAGTGGCAGATGGGCTGA
Protein sequence

>Msa0146000

MNEGLKKMGICFGCFGGGDKRMTKEEERLASEEARARAAEAAQKRQKDFEKSAAGRAARAHQKGMAKQAANSNKGEPTLKWQMG*