AlfalfaGEDB | Msa0152030

M. sativa cultivar zhongmu-4 / Msa0152030

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0152030	XP_024632725.2	84.789	710	44	6	1	647	13	721	0.0	1214

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0152030	sp\|Q9SY66\|FRS11_ARATH	34.581	561	295	9	50	542	48	604	1.49e-108	345

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0152030	A0A2U1NDE9	53.163	664	260	9	21	646	10	660	0.0	686

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TFs/TRs:

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Gene ID	Type	Classification
Msa0152030	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0152030	Msa0152070	0.871325	7.446633e-67	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0152030	MtrunA17_Chr2g0294841	46.828	331	167	4	50	373	45	373	3.34e-97	315
Msa0152030	MtrunA17_Chr2g0294841	27.530	247	108	7	438	614	500	745	1.40e-16	84.0
Msa0152030	MtrunA17_Chr2g0276841	45.260	327	173	3	50	372	70	394	1.12e-94	306
Msa0152030	MtrunA17_Chr2g0276841	32.639	144	89	4	403	542	487	626	1.89e-16	83.2
Msa0152030	MtrunA17_Chr2g0290391	32.743	565	305	16	50	543	51	611	4.07e-92	300
Msa0152030	MtrunA17_Chr5g0431001	45.509	334	169	4	50	373	61	391	5.07e-92	294
Msa0152030	MtrunA17_Chr1g0198761	42.727	330	176	4	53	372	50	376	9.94e-86	284
Msa0152030	MtrunA17_Chr1g0198761	29.670	273	137	5	404	622	470	741	6.58e-30	125
Msa0152030	MtrunA17_Chr5g0448761	29.289	577	320	15	46	543	56	623	1.10e-67	236
Msa0152030	MtrunA17_Chr1g0202341	30.070	572	311	15	48	543	49	607	9.82e-62	218

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0152030	AT1G10240.2	34.581	561	295	9	50	542	48	604	1.51e-109	345
Msa0152030	AT1G10240.1	34.581	561	295	9	50	542	48	604	1.51e-109	345
Msa0152030	AT5G28530.3	31.886	599	324	15	23	540	17	612	4.89e-97	312
Msa0152030	AT5G28530.2	31.886	599	324	15	23	540	17	612	4.89e-97	312
Msa0152030	AT5G28530.1	31.509	603	325	14	23	540	17	616	1.68e-96	311
Msa0152030	AT5G28530.5	29.711	589	287	17	23	540	17	549	2.66e-74	251
Msa0152030	AT5G28530.4	29.342	593	288	16	23	540	17	553	7.37e-74	249
Msa0152030	AT4G38180.1	29.549	599	333	14	23	543	45	632	1.66e-71	247
Msa0152030	AT1G76320.3	26.998	563	307	17	56	540	3	539	3.88e-54	197
Msa0152030	AT1G76320.2	26.998	563	307	17	56	540	3	539	4.18e-54	197

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Find 86 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAAAACGATCAAAAGG+AGG	0.648569	1_4:+14375049	Msa0152030:CDS
AAAACGATCAAAAGGAGGAA+TGG	0.539810	1_4:+14375054	Msa0152030:CDS
AAACAGTGTCTACATTTCAA+TGG	0.471539	1_4:+14374072	Msa0152030:CDS
AAAGACTAGAAAATATATTT+TGG	0.283353	1_4:+14373889	Msa0152030:CDS
AAGCATAAAGTCTTATGGGA+CGG	0.643257	1_4:+14374773	Msa0152030:CDS
AAGGTAGTAGATCATGATTG+TGG	0.693617	1_4:-14373572	None:intergenic
AATACTTATAATATGCCTTT+TGG	0.320115	1_4:+14373984	Msa0152030:CDS
AATAGACCATCTTCGAGATG+TGG	0.610642	1_4:+14373465	Msa0152030:CDS
AATATGATGCTTAGTGAAGG+AGG	0.615302	1_4:+14374953	Msa0152030:CDS
AATTGAGAACCCACTCCTTT+TGG	0.307636	1_4:-14373331	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTACGTCCAAACCAAATTTA+CGG	-	chr1_4:14372310-14372329	None:intergenic	30.0%
ACAACCCATTTATACTCATA+TGG	+	chr1_4:14372694-14372713	Msa0152030:intron	30.0%
GCAACCATATGAGTATAAAT+GGG	-	chr1_4:14372701-14372720	None:intergenic	30.0%
AGCAACCATATGAGTATAAA+TGG	-	chr1_4:14372702-14372721	None:intergenic	30.0%
ATTAAGGAATTAACTCACTC+AGG	-	chr1_4:14372728-14372747	None:intergenic	30.0%
ACATCATATTAAAAGGGAAC+AGG	-	chr1_4:14372957-14372976	None:intergenic	30.0%
CTATTTCGTTGTTCTTTAGA+TGG	-	chr1_4:14373450-14373469	None:intergenic	30.0%
TATTTGCTTGACTGAAAGAT+CGG	-	chr1_4:14373676-14373695	None:intergenic	30.0%
TGCATTTGATACTACTTACA+AGG	+	chr1_4:14373959-14373978	Msa0152030:CDS	30.0%
AAACAGTGTCTACATTTCAA+TGG	+	chr1_4:14374072-14374091	Msa0152030:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	14372215	14375246	14372215	ID=Msa0152030;Name=Msa0152030
chr1_4	mRNA	14372215	14375246	14372215	ID=Msa0152030-mRNA-1;Parent=Msa0152030;Name=Msa0152030-mRNA-1;_AED=0.07;_eAED=0.15;_QI=0\|0\|0\|0.75\|0.66\|0.5\|4\|0\|648
chr1_4	exon	14372215	14372231	14372215	ID=Msa0152030-mRNA-1:exon:5696;Parent=Msa0152030-mRNA-1
chr1_4	exon	14373140	14374103	14373140	ID=Msa0152030-mRNA-1:exon:5697;Parent=Msa0152030-mRNA-1
chr1_4	exon	14374218	14374472	14374218	ID=Msa0152030-mRNA-1:exon:5698;Parent=Msa0152030-mRNA-1
chr1_4	exon	14374536	14375246	14374536	ID=Msa0152030-mRNA-1:exon:5699;Parent=Msa0152030-mRNA-1
chr1_4	CDS	14372215	14372231	14372215	ID=Msa0152030-mRNA-1:cds;Parent=Msa0152030-mRNA-1
chr1_4	CDS	14373140	14374103	14373140	ID=Msa0152030-mRNA-1:cds;Parent=Msa0152030-mRNA-1
chr1_4	CDS	14374218	14374472	14374218	ID=Msa0152030-mRNA-1:cds;Parent=Msa0152030-mRNA-1
chr1_4	CDS	14374536	14375246	14374536	ID=Msa0152030-mRNA-1:cds;Parent=Msa0152030-mRNA-1

Gene Sequence

>Msa0152030

ATGTTGCTGGGAGCGCTGTTGAGAATCACAAATTTGGTTGATTTGAACAATCTTCCAAACAATGAACCATGTGGTAATTCAGAAAGTGGAGTAGTTGAGTTGAGTAGCAATGAACAAGAAGCAATTACTATTGAAAACGATTTTGAACCTTTTATAGGGCAGTGTTTTCTAAGTGAAGAAGAAGCTTTCAAATTTTATGAGAATTATGCCAAAAGGAGTGGGTTCTCAATTAGAAAAGGTAGGTTTGTTAATAAAAATGGGATAATGCATAGGAGAGATTTGTTTTGTCACCGTGGAGGATTTCCTGATAAAAAAATTCAAGATCCATCTAAAGAACAACGAAATAGACCATCTTCGAGATGTGGATGCAAGGCAAGAATGACAATCAAATTGAAGAAATCCTTTGAGATTTTTCCAGAAGAGTGGCAGGTTATTGAATTTATATCTAACCACAATCATGATCTACTACCTTCAGATCAAGTGCACTTCCTTGCATCCTACCGCAAAATTTCTAAGGAAGAAGAGAAGCGGATATTGCTATTAAAAGAAGCCGATCTTTCAGTCAAGCAAATAATGCGTGTTATGGAGCTTGAGAAAAATGTAAAACATGGAGATCTTCGATTTCTTGAGAAAGATATTCATAATTTCTATAATAAGATTCATCAAGATAACATGAATGGTGATGCTAATGATCTTTTACAACTTTGTAAGTTTTCCAAGGAAGAAAACTCATTCTTCCAATTTGAATTTACGGTTGATAAAGAAAAAAGACTAGAAAATATATTTTGGTCTCCATCTCATTGTTTTGAATTGTACCAACGGTATGGAGATGCTGTTGCATTTGATACTACTTACAAGGTAAATACTTATAATATGCCTTTTGGCATTTTTGTTGGAGTCGACAATCACGGCAGAACAATATTGTTTGGTTGTGCGCTCATTCGAAATGAAACAGTGTCTACATTTCAATGGTTAATGAAAACTTTTGTTACTCTCATGAAGAAGCCTCCAAAGACAATCATTACTGATCAAGATCCATGGATGACTCAAGATATTGCTTTTGAAATGCCATCCACAAAACATGCTTTTTGTATATGGCACATTACTGCTAAATTTAGTGGGCATGACAACAACTGGAAGATCAGAGAGCATAAATGGATTCCTAAAACGCTTTACTTCATCTCGTTCATGTTTAACTCAACTTTTCAAACAAGTAAGAATATGTATGATAATTTAGTTGGTCTTGCCATTGAGGACATTGGGCAAAAACAATTACATCATACCATGTTGGATACATACAGGGGATCATCACTTCGATCTTTATCCCCTTTAGAGGAGCAAGTTTACAATATTTTCACAAAATATGCTTTCAAGAAATTTCAGGAGGAGTTTGAGAGGGCAACTCAATACAAGATTTGTGAAGAAAATCATGTAGAATTTACTGTGAAATATTACAAGGAGCAACATTCACAAAAGCATAAAGTCTTATGGGACGGTGATGTGGTTGGTTGTAGTTGCAAGCACTTTGAGTTTTGGGGTATTCTTTGTCGTCATGTATTGACAATATTCCTTCATAAAGATTGTTTTGAGATTCCAACCAGTTACTTGCCATTACGTTGGTGTCGTGATGAATTTCAAAGACCGGTTGAAGTAAATATGATGCTTAGTGAAGGAGGACCTATTGAGAATGTGGAGAATACAGTAGACTTAATTAAAAATCCGCCAAGGTCTAATACAAAAGGTCGTCCCAAAACAAAACGATCAAAAGGAGGAATGGAATTGTCAAAGAGAACAAGAAGCTGTACTTTTTGCAAGAGAAGGGGGCACAATGTTACAACTTGTCCGGACAAAGAAAGTCATGTTCAGCCAACAATCACCAAGAAAAGGAAGAATGAAGATCAAGCACAACAAAATTTGAATCCTGTTTTTTATCTAAAGTCTTCTTAG

Protein sequence

>Msa0152030

MLLGALLRITNLVDLNNLPNNEPCGNSESGVVELSSNEQEAITIENDFEPFIGQCFLSEEEAFKFYENYAKRSGFSIRKGRFVNKNGIMHRRDLFCHRGGFPDKKIQDPSKEQRNRPSSRCGCKARMTIKLKKSFEIFPEEWQVIEFISNHNHDLLPSDQVHFLASYRKISKEEEKRILLLKEADLSVKQIMRVMELEKNVKHGDLRFLEKDIHNFYNKIHQDNMNGDANDLLQLCKFSKEENSFFQFEFTVDKEKRLENIFWSPSHCFELYQRYGDAVAFDTTYKVNTYNMPFGIFVGVDNHGRTILFGCALIRNETVSTFQWLMKTFVTLMKKPPKTIITDQDPWMTQDIAFEMPSTKHAFCIWHITAKFSGHDNNWKIREHKWIPKTLYFISFMFNSTFQTSKNMYDNLVGLAIEDIGQKQLHHTMLDTYRGSSLRSLSPLEEQVYNIFTKYAFKKFQEEFERATQYKICEENHVEFTVKYYKEQHSQKHKVLWDGDVVGCSCKHFEFWGILCRHVLTIFLHKDCFEIPTSYLPLRWCRDEFQRPVEVNMMLSEGGPIENVENTVDLIKNPPRSNTKGRPKTKRSKGGMELSKRTRSCTFCKRRGHNVTTCPDKESHVQPTITKKRKNEDQAQQNLNPVFYLKSS*