AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0160450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0160450 MtrunA17_Chr1g0173541 69.192 198 38 3 2 187 196 382 7.13e-89 270
Msa0160450 MtrunA17_Chr7g0257751 70.312 192 46 3 2 187 202 388 1.10e-86 264
Msa0160450 MtrunA17_Chr4g0059951 58.586 198 59 3 2 187 217 403 1.65e-71 226
Msa0160450 MtrunA17_Chr7g0242561 58.081 198 60 3 2 187 200 386 7.37e-70 221
Msa0160450 MtrunA17_Chr5g0448721 52.632 190 83 3 2 187 240 426 1.24e-60 197
Msa0160450 MtrunA17_Chr8g0385341 54.167 192 73 3 4 187 176 360 2.32e-60 195
Msa0160450 MtrunA17_Chr4g0033371 51.852 189 69 2 11 188 226 403 2.34e-60 196
Msa0160450 MtrunA17_Chr1g0176751 51.813 193 80 3 2 187 268 454 4.86e-60 196
Msa0160450 MtrunA17_Chr0c02g0489761 53.299 197 69 3 4 188 224 409 6.09e-60 195
Msa0160450 MtrunA17_Chr1g0170281 55.080 187 62 3 12 187 273 448 8.30e-60 196
Msa0160450 MtrunA17_Chr3g0131181 52.406 187 88 1 2 187 173 359 1.58e-59 194
Msa0160450 MtrunA17_Chr5g0397101 51.795 195 72 3 4 187 213 396 2.73e-59 193
Msa0160450 MtrunA17_Chr1g0176421 51.010 198 74 4 2 187 305 491 3.49e-59 195
Msa0160450 MtrunA17_Chr5g0441691 50.254 197 75 3 4 188 214 399 1.12e-58 191
Msa0160450 MtrunA17_Chr1g0200601 52.432 185 87 1 4 187 176 360 1.50e-58 191
Msa0160450 MtrunA17_Chr8g0387671 51.309 191 80 3 4 187 212 396 1.05e-57 189
Msa0160450 MtrunA17_Chr4g0074811 49.746 197 76 4 4 188 237 422 1.16e-56 186
Msa0160450 MtrunA17_Chr1g0157131 50.254 197 75 3 4 188 220 405 1.34e-55 183
Msa0160450 MtrunA17_Chr3g0098951 50.769 195 74 3 4 187 230 413 2.09e-55 183
Msa0160450 MtrunA17_Chr5g0444171 50.000 196 75 3 4 187 238 422 2.48e-54 180
Msa0160450 MtrunA17_Chr7g0267751 47.938 194 86 3 2 187 279 465 1.92e-53 179
Msa0160450 MtrunA17_Chr4g0074791 44.974 189 81 4 11 188 250 426 2.17e-45 156
Msa0160450 MtrunA17_Chr5g0405421 44.505 182 91 2 12 188 256 432 1.77e-42 149
Msa0160450 MtrunA17_Chr5g0444191 64.000 100 36 0 9 108 60 159 7.38e-42 140
Msa0160450 MtrunA17_Chr5g0402551 39.011 182 98 2 13 188 294 468 1.64e-39 141
Msa0160450 MtrunA17_Chr7g0267771 38.378 185 100 3 2 183 232 405 4.23e-38 136
Msa0160450 MtrunA17_Chr8g0374701 38.378 185 93 3 13 187 288 461 1.31e-37 136
Msa0160450 MtrunA17_Chr4g0045261 37.569 181 100 2 13 187 297 470 1.34e-36 133
Msa0160450 MtrunA17_Chr7g0256001 38.202 178 104 2 13 187 301 475 2.25e-36 133
Msa0160450 MtrunA17_Chr7g0276111 34.848 198 108 4 1 188 291 477 1.91e-35 130
Msa0160450 MtrunA17_Chr3g0127571 38.043 184 93 4 13 187 298 469 6.04e-35 129
Msa0160450 MtrunA17_Chr1g0203881 43.307 127 60 1 12 138 196 310 3.39e-28 109
Msa0160450 MtrunA17_Chr1g0190131 41.176 102 59 1 9 109 157 258 1.22e-24 97.1
Msa0160450 MtrunA17_Chr3g0132591 40.187 107 63 1 4 109 248 354 1.63e-20 88.2
Msa0160450 MtrunA17_Chr7g0274941 40.000 100 58 1 12 109 169 268 3.34e-20 85.5
Msa0160450 MtrunA17_Chr4g0069301 38.393 112 59 5 4 109 168 275 1.71e-15 73.6
Msa0160450 MtrunA17_Chr2g0304111 38.393 112 59 5 4 109 165 272 8.41e-15 71.6
Msa0160450 MtrunA17_Chr2g0302431 43.820 89 44 4 19 105 181 265 1.33e-14 71.2
Msa0160450 MtrunA17_Chr6g0478601 40.404 99 49 3 19 112 301 394 3.38e-14 70.1
Msa0160450 MtrunA17_Chr8g0355131 33.803 142 72 4 18 148 208 338 2.33e-13 67.4
Msa0160450 MtrunA17_Chr5g0441771 35.537 121 57 3 64 184 2 101 3.61e-13 62.8
Msa0160450 MtrunA17_Chr4g0001331 35.714 112 62 4 4 109 161 268 1.01e-12 65.5
Msa0160450 MtrunA17_Chr6g0453681 36.697 109 62 3 4 109 161 265 1.90e-12 64.7
Msa0160450 MtrunA17_Chr6g0468751 36.697 109 62 3 4 109 161 265 2.01e-12 64.7
Msa0160450 MtrunA17_Chr4g0008561 37.634 93 55 3 18 108 178 269 2.44e-12 64.7
Msa0160450 MtrunA17_Chr4g0063881 39.286 112 56 7 4 109 153 258 3.12e-12 64.3
Msa0160450 MtrunA17_Chr3g0132671 36.000 100 58 3 12 109 200 295 6.19e-12 63.5
Msa0160450 MtrunA17_Chr1g0167311 38.776 98 57 3 18 114 315 410 8.97e-12 62.8
Msa0160450 MtrunA17_Chr1g0166101 39.785 93 50 4 19 109 185 273 1.23e-11 62.4
Msa0160450 MtrunA17_Chr4g0018201 42.045 88 49 2 18 104 947 1033 1.28e-11 62.8
Msa0160450 MtrunA17_Chr8g0353621 31.776 107 69 3 4 109 152 255 1.63e-11 61.6
Msa0160450 MtrunA17_Chr5g0428981 42.045 88 49 2 18 104 951 1037 1.89e-11 62.0
Msa0160450 MtrunA17_Chr8g0351491 33.333 93 59 2 18 109 171 261 5.83e-11 60.1
Msa0160450 MtrunA17_Chr4g0059751 32.110 109 67 4 1 106 234 338 7.61e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0160450 AT3G57530.2 64.646 198 47 3 2 187 207 393 2.29e-82 250
Msa0160450 AT3G57530.1 64.646 198 47 3 2 187 207 393 1.88e-81 251
Msa0160450 AT5G12480.2 65.608 189 60 3 2 187 109 295 1.30e-79 244
Msa0160450 AT5G12480.1 65.608 189 60 3 2 187 203 389 1.58e-78 244
Msa0160450 AT2G41860.1 67.725 189 56 3 2 187 198 384 5.31e-78 242
Msa0160450 AT5G19450.2 65.608 189 60 3 2 187 201 387 1.30e-77 241
Msa0160450 AT5G19450.1 65.608 189 60 3 2 187 201 387 1.30e-77 241
Msa0160450 AT2G41860.2 67.196 189 57 3 2 187 198 384 1.52e-77 241
Msa0160450 AT3G51850.3 58.586 198 59 3 2 187 198 384 2.27e-71 222
Msa0160450 AT3G51850.2 58.586 198 59 3 2 187 198 384 1.03e-70 223
Msa0160450 AT3G51850.1 58.586 198 59 3 2 187 198 384 3.84e-70 222
Msa0160450 AT1G74740.1 57.576 198 61 3 2 187 203 389 6.41e-68 216
Msa0160450 AT1G18890.1 54.545 198 67 3 2 187 207 393 1.39e-65 210
Msa0160450 AT4G35310.2 52.105 190 84 3 2 187 241 427 1.49e-60 197
Msa0160450 AT4G35310.1 52.105 190 84 3 2 187 241 427 1.49e-60 197
Msa0160450 AT4G38230.1 51.053 190 86 3 2 187 24 210 2.86e-60 191
Msa0160450 AT4G09570.1 54.737 190 75 4 4 187 171 355 2.88e-60 195
Msa0160450 AT2G31500.1 51.515 198 73 3 2 187 210 396 3.37e-60 197
Msa0160450 AT4G23650.1 54.545 187 63 3 12 187 233 408 3.45e-60 196
Msa0160450 AT2G17290.1 52.105 190 84 3 2 187 229 415 5.00e-60 196
Msa0160450 AT2G17290.2 52.105 190 84 3 2 187 229 415 5.00e-60 196
Msa0160450 AT4G38230.4 51.053 190 86 3 2 187 168 354 8.83e-59 191
Msa0160450 AT4G38230.3 51.053 190 86 3 2 187 168 354 8.83e-59 191
Msa0160450 AT3G10660.2 51.010 198 74 4 2 187 330 516 1.16e-58 194
Msa0160450 AT3G10660.1 51.010 198 74 4 2 187 330 516 1.16e-58 194
Msa0160450 AT4G38230.2 51.053 190 86 3 2 187 198 384 1.46e-58 191
Msa0160450 AT1G35670.1 51.020 196 73 4 4 187 172 356 7.34e-58 189
Msa0160450 AT5G12180.1 53.191 188 66 2 12 188 228 404 1.90e-57 189
Msa0160450 AT5G04870.1 50.000 198 76 4 2 187 294 480 2.65e-57 190
Msa0160450 AT1G50700.3 52.083 192 79 3 4 188 219 404 5.86e-57 186
Msa0160450 AT2G38910.1 50.000 188 93 1 2 188 278 465 6.13e-57 188
Msa0160450 AT1G50700.1 52.083 192 79 3 4 188 219 404 9.22e-57 187
Msa0160450 AT1G50700.2 52.083 192 79 3 4 188 219 404 1.63e-56 186
Msa0160450 AT3G20410.2 49.239 197 77 4 4 188 237 422 1.11e-55 184
Msa0160450 AT3G20410.1 49.239 197 77 4 4 188 237 422 1.11e-55 184
Msa0160450 AT5G19360.1 50.761 197 74 3 4 188 214 399 1.57e-55 183
Msa0160450 AT1G76040.1 51.648 182 75 2 12 187 29 203 2.26e-55 178
Msa0160450 AT1G76040.4 51.648 182 75 2 12 187 29 203 2.26e-55 178
Msa0160450 AT4G04720.2 50.000 196 76 3 4 188 226 410 1.09e-54 181
Msa0160450 AT4G04720.1 50.000 196 76 3 4 188 226 410 1.09e-54 181
Msa0160450 AT1G76040.3 50.262 191 81 3 4 187 258 441 6.09e-54 179
Msa0160450 AT2G35890.2 46.970 198 82 4 2 187 276 462 7.52e-54 179
Msa0160450 AT2G35890.1 46.970 198 82 4 2 187 276 462 7.52e-54 179
Msa0160450 AT1G76040.2 50.262 191 81 3 4 187 258 441 1.20e-53 179
Msa0160450 AT5G23580.1 49.223 193 75 4 4 184 168 349 1.35e-53 177
Msa0160450 AT4G04740.4 49.490 196 77 3 4 188 215 399 8.39e-53 176
Msa0160450 AT4G04740.6 49.490 196 77 3 4 188 215 399 8.39e-53 176
Msa0160450 AT4G04740.5 49.490 196 77 3 4 188 215 399 8.39e-53 176
Msa0160450 AT4G04740.3 49.490 196 77 3 4 188 215 399 8.39e-53 176
Msa0160450 AT4G04740.1 49.490 196 77 3 4 188 215 399 8.39e-53 176
Msa0160450 AT4G04740.7 49.490 196 77 3 4 188 215 399 8.39e-53 176
Msa0160450 AT4G04740.2 49.490 196 77 3 4 188 215 399 9.54e-53 176
Msa0160450 AT4G21940.3 47.917 192 86 3 4 188 248 432 1.29e-51 172
Msa0160450 AT4G21940.1 47.917 192 86 3 4 188 248 432 3.48e-51 172
Msa0160450 AT4G21940.2 47.917 192 86 3 4 188 248 432 4.34e-51 172
Msa0160450 AT1G61950.1 47.959 196 79 4 4 187 245 429 5.99e-50 169
Msa0160450 AT5G66210.6 42.021 188 87 2 12 188 219 395 8.07e-43 148
Msa0160450 AT5G66210.4 42.021 188 87 2 12 188 219 395 8.07e-43 148
Msa0160450 AT5G66210.5 41.117 197 93 3 4 188 210 395 1.96e-42 148
Msa0160450 AT5G66210.3 41.117 197 93 3 4 188 210 395 2.59e-42 148
Msa0160450 AT5G66210.1 41.117 197 93 3 4 188 210 395 3.16e-42 148
Msa0160450 AT5G66210.2 41.117 197 93 3 4 188 210 395 3.16e-42 148
Msa0160450 AT2G17890.1 41.885 191 100 3 4 188 256 441 1.95e-41 147
Msa0160450 AT4G04710.2 43.655 197 84 4 2 188 184 363 2.47e-41 145
Msa0160450 AT4G04710.3 43.655 197 84 4 2 188 170 349 2.47e-41 145
Msa0160450 AT4G04710.1 41.667 204 91 4 2 188 184 376 4.64e-40 142
Msa0160450 AT4G04710.4 41.667 204 91 4 2 188 184 376 4.77e-40 142
Msa0160450 AT4G36070.1 40.659 182 98 2 12 188 228 404 1.26e-39 141
Msa0160450 AT3G50530.2 40.000 185 90 3 13 187 339 512 1.46e-39 142
Msa0160450 AT3G50530.1 40.000 185 90 3 13 187 308 481 1.63e-39 142
Msa0160450 AT4G36070.2 39.362 188 92 2 12 188 228 404 1.69e-39 141
Msa0160450 AT4G04700.1 40.201 199 97 3 1 188 175 362 4.55e-38 136
Msa0160450 AT1G49580.1 37.968 187 94 2 13 188 310 485 5.39e-37 135
Msa0160450 AT4G04695.1 41.414 198 94 3 2 188 176 362 1.19e-36 133
Msa0160450 AT3G19100.1 38.798 183 97 3 13 188 304 478 2.09e-36 133
Msa0160450 AT2G41140.1 39.665 179 99 3 13 187 283 456 5.41e-36 132
Msa0160450 AT3G56760.1 39.106 179 100 3 13 187 284 457 8.15e-36 131
Msa0160450 AT3G49370.1 34.946 186 100 3 1 176 289 463 1.11e-32 123
Msa0160450 AT5G24430.1 35.838 173 91 2 13 176 303 464 1.63e-32 122
Msa0160450 AT2G46700.2 49.533 107 53 1 1 106 109 215 1.84e-31 118
Msa0160450 AT2G46700.3 49.533 107 53 1 1 106 109 215 1.84e-31 118
Msa0160450 AT2G46700.1 49.533 107 53 1 1 106 290 396 1.86e-30 117
Msa0160450 AT1G12580.1 48.980 98 50 0 12 109 199 296 4.12e-27 107
Msa0160450 AT1G08650.2 43.269 104 58 1 9 111 166 269 5.11e-24 95.9
Msa0160450 AT1G08650.1 43.137 102 57 1 9 109 166 267 7.53e-24 95.5
Msa0160450 AT1G12680.1 42.727 110 62 1 1 109 240 349 1.89e-23 96.7
Msa0160450 AT3G04530.1 40.909 110 64 1 1 109 154 263 6.47e-22 90.1
Msa0160450 AT1G30270.1 38.393 112 59 5 4 109 173 280 3.32e-15 73.2
Msa0160450 AT1G30270.2 38.393 112 59 5 4 109 173 280 3.47e-15 73.2
Msa0160450 AT1G30270.3 38.393 112 59 5 4 109 76 183 4.02e-15 72.8
Msa0160450 AT5G39440.1 37.838 111 63 3 1 109 158 264 5.57e-15 72.8
Msa0160450 AT5G10930.1 43.820 89 44 3 19 105 173 257 1.15e-14 71.6
Msa0160450 AT1G48260.1 39.450 109 54 5 4 105 153 256 6.67e-14 69.3
Msa0160450 AT1G48260.2 39.450 109 54 5 4 105 68 171 7.33e-14 68.9
Msa0160450 AT1G48260.4 39.450 109 54 5 4 105 68 171 7.33e-14 68.9
Msa0160450 AT1G48260.3 39.450 109 54 5 4 105 68 171 7.33e-14 68.9
Msa0160450 AT1G48260.5 39.450 109 54 5 4 105 68 171 7.33e-14 68.9
Msa0160450 AT3G17510.2 40.367 109 53 5 4 105 82 185 7.83e-14 68.9
Msa0160450 AT5G01810.3 33.929 112 64 4 4 109 153 260 2.45e-13 67.8
Msa0160450 AT5G01810.1 33.929 112 64 4 4 109 153 260 2.54e-13 67.8
Msa0160450 AT5G01810.2 33.929 112 64 4 4 109 153 260 2.54e-13 67.8
Msa0160450 AT3G53930.1 37.500 96 51 3 18 108 179 270 2.92e-13 67.8
Msa0160450 AT3G53930.2 37.500 96 51 3 18 108 179 270 2.97e-13 67.8
Msa0160450 AT5G62310.1 42.553 94 44 3 18 106 943 1031 3.60e-13 67.4
Msa0160450 AT3G29160.3 37.615 109 61 3 4 109 162 266 6.89e-13 66.2
Msa0160450 AT1G48490.3 41.111 90 51 2 19 107 1012 1100 7.33e-13 66.6
Msa0160450 AT1G48490.2 41.111 90 51 2 19 107 1012 1100 7.33e-13 66.6
Msa0160450 AT1G48490.1 41.111 90 51 2 19 107 1012 1100 7.33e-13 66.6
Msa0160450 AT1G48490.4 41.111 90 51 2 19 107 1013 1101 7.40e-13 66.6
Msa0160450 AT5G25110.1 42.697 89 45 4 19 105 203 287 7.85e-13 66.2
Msa0160450 AT3G29160.1 37.615 109 61 3 4 109 162 266 9.20e-13 66.2
Msa0160450 AT3G29160.2 37.615 109 61 3 4 109 162 266 9.20e-13 66.2
Msa0160450 AT3G01090.3 37.615 109 61 3 4 109 161 265 1.16e-12 65.9
Msa0160450 AT3G01090.1 37.615 109 61 3 4 109 161 265 1.16e-12 65.9
Msa0160450 AT3G01090.2 37.615 109 61 3 4 109 184 288 1.21e-12 65.9
Msa0160450 AT3G17850.1 39.362 94 47 3 19 107 1072 1160 1.32e-12 65.9
Msa0160450 AT1G45160.1 38.298 94 48 3 19 107 856 944 1.51e-12 65.9
Msa0160450 AT1G45160.2 38.298 94 48 3 19 107 856 944 1.57e-12 65.9
Msa0160450 AT2G26980.2 38.835 103 53 5 4 100 156 254 2.31e-11 61.2
Msa0160450 AT2G20050.1 36.364 99 56 3 12 107 932 1026 2.52e-11 62.0
Msa0160450 AT2G20050.2 36.364 99 56 3 12 107 929 1023 2.54e-11 62.0
Msa0160450 AT1G01140.1 36.559 93 53 4 19 109 180 268 2.68e-11 62.0
Msa0160450 AT1G01140.3 36.559 93 53 4 19 109 180 268 2.69e-11 62.0
Msa0160450 AT2G45490.1 33.333 93 59 2 18 109 177 267 3.94e-11 60.8
Msa0160450 AT5G09890.1 41.772 79 45 1 19 96 305 383 5.63e-11 60.8
Msa0160450 AT5G09890.2 41.772 79 45 1 19 96 305 383 5.91e-11 60.8

Find 58 sgRNAs with CRISPR-Local

Find 288 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACATCTTCCATTAGTATT+TGG 0.177416 1_4:+32907875 None:intergenic
ATTCTTTACATCTTACTTTC+TGG 0.208402 1_4:-32909162 Msa0160450:CDS
GTGAGTATATTCCCATTATC+TGG 0.214914 1_4:-32907856 Msa0160450:three_prime_UTR
ACTTCAGGGGCCATGTAATA+TGG 0.287926 1_4:+32909234 None:intergenic
GCACTCCAGATATCAATTTC+AGG 0.289113 1_4:+32909192 None:intergenic
GTGCAGTAAGCCGGCGCTTT+GGG 0.289898 1_4:+32908411 None:intergenic
ACAGAATAGATCCCAGATAA+TGG 0.293405 1_4:+32907844 None:intergenic
TGTGCAGTAAGCCGGCGCTT+TGG 0.327314 1_4:+32908410 None:intergenic
GAAAATTGTGTGAGCCTTGC+TGG 0.331474 1_4:+32908203 None:intergenic
GATGGATGACTTTCTATTAC+CGG 0.338055 1_4:+32907731 None:intergenic
ATATATATCATGTTAACATT+TGG 0.353145 1_4:-32909676 None:intergenic
ACATTTGGAGTTAAAATGTT+AGG 0.358434 1_4:-32909661 Msa0160450:CDS
AAATCCTCCTAATGTTTCAT+TGG 0.363649 1_4:-32908241 Msa0160450:CDS
TTCTTTGCATTCTGCAACCA+TGG 0.369376 1_4:+32908262 None:intergenic
TTTCATTAGTTCTATTTAAC+CGG 0.375769 1_4:-32907750 Msa0160450:three_prime_UTR
GAAAGATTTAACGAGATAGT+TGG 0.378078 1_4:-32909261 Msa0160450:CDS
GTTTCTCCCAATGAAACATT+AGG 0.386917 1_4:+32908234 None:intergenic
GGACATCTCCATCAGGAATT+CGG 0.387321 1_4:+32907896 None:intergenic
GTGATATATACAGAAAATAA+AGG 0.404149 1_4:-32907963 Msa0160450:CDS
CAGAATAGATCCCAGATAAT+GGG 0.406441 1_4:+32907845 None:intergenic
GTAAGCCGGCGCTTTGGGTC+AGG 0.420975 1_4:+32908416 None:intergenic
ATGGCCCTGAAATTGATATC+TGG 0.424350 1_4:-32909197 Msa0160450:CDS
TGGAGATGTCCAAATACTAA+TGG 0.429065 1_4:-32907884 Msa0160450:three_prime_UTR
TTGTGTGAGCCTTGCTGGCT+CGG 0.430581 1_4:+32908208 None:intergenic
AATCCTCCTAATGTTTCATT+GGG 0.441297 1_4:-32908240 Msa0160450:CDS
TTCGCATTATCAGAAACCTT+TGG 0.449362 1_4:+32908464 None:intergenic
AGAAGTTGTTAGAATAAAAG+AGG 0.455947 1_4:-32907994 Msa0160450:CDS
TTAAAATGCAGAAACTGAGC+AGG 0.461264 1_4:-32908540 Msa0160450:intron
TACTCATGTTGACAGTTAGT+TGG 0.462071 1_4:+32907782 None:intergenic
AAAGCGCCGGCTTACTGCAC+AGG 0.477508 1_4:-32908408 Msa0160450:CDS
GAAGTTGTTAGAATAAAAGA+GGG 0.499193 1_4:-32907993 Msa0160450:CDS
GTAATAGAAAGTCATCCATC+TGG 0.499711 1_4:-32907728 Msa0160450:three_prime_UTR
CACTCCAGATATCAATTTCA+GGG 0.504258 1_4:+32909193 None:intergenic
TGCTTATATTCAAGACCAGA+TGG 0.507162 1_4:+32907713 None:intergenic
GAAATTGATATCTGGAGTGC+TGG 0.520506 1_4:-32909189 Msa0160450:CDS
TCTGTTTGTTCCACAGTCGA+TGG 0.526269 1_4:-32907826 Msa0160450:three_prime_UTR
TTATCAGAAACCTTTGGCCA+TGG 0.532002 1_4:+32908470 None:intergenic
TTGACTTTACAAGGGTTCCA+TGG 0.534211 1_4:-32908487 Msa0160450:CDS
AGTATTTGGACATCTCCATC+AGG 0.535929 1_4:+32907889 None:intergenic
TGTTCCACAGTCGATGGATG+TGG 0.548598 1_4:-32907820 Msa0160450:three_prime_UTR
TGAGTATATTCCCATTATCT+GGG 0.548769 1_4:-32907855 Msa0160450:three_prime_UTR
AACTAAATGCAGATCATCCA+TGG 0.555527 1_4:-32908279 Msa0160450:intron
CTAGGTCACCGAATTCCTGA+TGG 0.557144 1_4:-32907904 Msa0160450:exon
TACAAGGGTTCCATGGCCAA+AGG 0.558449 1_4:-32908480 Msa0160450:CDS
ACATTGATGAACTACGAGTA+GGG 0.566646 1_4:-32907932 Msa0160450:CDS
TCTCCCAATGAAACATTAGG+AGG 0.570722 1_4:+32908237 None:intergenic
ACACTTCCTGTGCAGTAAGC+CGG 0.574215 1_4:+32908402 None:intergenic
AGTTGGAAGTCCATATTACA+TGG 0.582119 1_4:-32909244 Msa0160450:CDS
ACTTAAGAAAACAGCACTGA+GGG 0.590032 1_4:-32908168 Msa0160450:intron
TTGATCCTGACCCAAAGCGC+CGG 0.592831 1_4:-32908421 Msa0160450:CDS
AACATTGATGAACTACGAGT+AGG 0.607687 1_4:-32907933 Msa0160450:CDS
GGAGTTAAAATGTTAGGACA+AGG 0.611266 1_4:-32909655 Msa0160450:intron
ACTACGAGTAGGGTTAAGCT+AGG 0.621790 1_4:-32907922 Msa0160450:exon
ATTTCCACATCCATCGACTG+TGG 0.626550 1_4:+32907816 None:intergenic
GATCAAGCATCTTCTTAACA+AGG 0.646363 1_4:+32908438 None:intergenic
TAAAATGCAGAAACTGAGCA+GGG 0.661481 1_4:-32908539 Msa0160450:intron
AGAAACAGTCCGAGCCAGCA+AGG 0.667822 1_4:-32908217 Msa0160450:CDS
AACTTAAGAAAACAGCACTG+AGG 0.760635 1_4:-32908169 Msa0160450:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTATTTTCCTATAT+TGG - chr1_4:32909278-32909297 Msa0160450:intron 10.0%
!!! TTTATTTTATTTTCCTATAT+TGG - chr1_4:32909278-32909297 Msa0160450:intron 10.0%
!!! AATTTCTTTTTAATACTTCA+GGG + chr1_4:32908120-32908139 None:intergenic 15.0%
!!! TAATTTCTTTTTAATACTTC+AGG + chr1_4:32908121-32908140 None:intergenic 15.0%
!!! GAAGTATTAAAAAGAAATTA+TGG - chr1_4:32908121-32908140 Msa0160450:intron 15.0%
!! AATATACAAATGTAAATACA+AGG + chr1_4:32908655-32908674 None:intergenic 15.0%
!!! AATTTCTTTTTAATACTTCA+GGG + chr1_4:32908120-32908139 None:intergenic 15.0%
!!! TAATTTCTTTTTAATACTTC+AGG + chr1_4:32908121-32908140 None:intergenic 15.0%
!!! GAAGTATTAAAAAGAAATTA+TGG - chr1_4:32908121-32908140 Msa0160450:intron 15.0%
!! AATATACAAATGTAAATACA+AGG + chr1_4:32908655-32908674 None:intergenic 15.0%
!!! TAAAGTTTACAATTTTGTGT+AGG - chr1_4:32908052-32908071 Msa0160450:intron 20.0%
!!! ATTTCTTTTTAATACTTCAG+GGG + chr1_4:32908119-32908138 None:intergenic 20.0%
!!! ATCTTTTATTGACTTTACAA+GGG - chr1_4:32908842-32908861 Msa0160450:intron 20.0%
!! TAAAAAAGACCGAAATAAAA+GGG + chr1_4:32909262-32909281 None:intergenic 20.0%
!! ATAAAAAAGACCGAAATAAA+AGG + chr1_4:32909263-32909282 None:intergenic 20.0%
!!! TATTTTCTGTATATATCACT+CGG + chr1_4:32909373-32909392 None:intergenic 20.0%
!! GTGATATATACAGAAAATAA+AGG - chr1_4:32909374-32909393 Msa0160450:intron 20.0%
!!! TTTCATTAGTTCTATTTAAC+CGG - chr1_4:32909587-32909606 Msa0160450:intron 20.0%
!!! CATTTATCTTTTTCATTTTG+TGG + chr1_4:32909668-32909687 None:intergenic 20.0%
!!! TAAAGTTTACAATTTTGTGT+AGG - chr1_4:32908052-32908071 Msa0160450:intron 20.0%
!!! ATTTCTTTTTAATACTTCAG+GGG + chr1_4:32908119-32908138 None:intergenic 20.0%
!!! ATCTTTTATTGACTTTACAA+GGG - chr1_4:32908842-32908861 Msa0160450:intron 20.0%
!! TAAAAAAGACCGAAATAAAA+GGG + chr1_4:32909262-32909281 None:intergenic 20.0%
!! ATAAAAAAGACCGAAATAAA+AGG + chr1_4:32909263-32909282 None:intergenic 20.0%
!!! TATTTTCTGTATATATCACT+CGG + chr1_4:32909373-32909392 None:intergenic 20.0%
!! GTGATATATACAGAAAATAA+AGG - chr1_4:32909374-32909393 Msa0160450:intron 20.0%
!!! TTTCATTAGTTCTATTTAAC+CGG - chr1_4:32909587-32909606 Msa0160450:intron 20.0%
!!! CATTTATCTTTTTCATTTTG+TGG + chr1_4:32909668-32909687 None:intergenic 20.0%
!! ACATTTGGAGTTAAAATGTT+AGG - chr1_4:32907676-32907695 Msa0160450:three_prime_UTR 25.0%
! ATATATCATAACCAACAGAA+GGG + chr1_4:32907777-32907796 None:intergenic 25.0%
! AATATATCATAACCAACAGA+AGG + chr1_4:32907778-32907797 None:intergenic 25.0%
!! TTCTGTTGGTTATGATATAT+TGG - chr1_4:32907777-32907796 Msa0160450:three_prime_UTR 25.0%
!! TCTGTTGGTTATGATATATT+GGG - chr1_4:32907778-32907797 Msa0160450:three_prime_UTR 25.0%
! AAACTTTAGTTAACTGTGTA+TGG + chr1_4:32908040-32908059 None:intergenic 25.0%
! ATTCTTTACATCTTACTTTC+TGG - chr1_4:32908175-32908194 Msa0160450:CDS 25.0%
! CACTTTAACCTAAATTCTTA+AGG + chr1_4:32908365-32908384 None:intergenic 25.0%
!! AACTACTCTAAAAAAAGAGT+AGG + chr1_4:32908491-32908510 None:intergenic 25.0%
! TTACTATAACCTACTTGAAA+GGG - chr1_4:32908509-32908528 Msa0160450:CDS 25.0%
!!! GATCTTTTATTGACTTTACA+AGG - chr1_4:32908841-32908860 Msa0160450:intron 25.0%
! AGAAGTTGTTAGAATAAAAG+AGG - chr1_4:32909343-32909362 Msa0160450:intron 25.0%
! GAAGTTGTTAGAATAAAAGA+GGG - chr1_4:32909344-32909363 Msa0160450:intron 25.0%
!! ACATTTGGAGTTAAAATGTT+AGG - chr1_4:32907676-32907695 Msa0160450:three_prime_UTR 25.0%
! ATATATCATAACCAACAGAA+GGG + chr1_4:32907777-32907796 None:intergenic 25.0%
! AATATATCATAACCAACAGA+AGG + chr1_4:32907778-32907797 None:intergenic 25.0%
!! TTCTGTTGGTTATGATATAT+TGG - chr1_4:32907777-32907796 Msa0160450:three_prime_UTR 25.0%
!! TCTGTTGGTTATGATATATT+GGG - chr1_4:32907778-32907797 Msa0160450:three_prime_UTR 25.0%
! AAACTTTAGTTAACTGTGTA+TGG + chr1_4:32908040-32908059 None:intergenic 25.0%
! ATTCTTTACATCTTACTTTC+TGG - chr1_4:32908175-32908194 Msa0160450:CDS 25.0%
! CACTTTAACCTAAATTCTTA+AGG + chr1_4:32908365-32908384 None:intergenic 25.0%
!! AACTACTCTAAAAAAAGAGT+AGG + chr1_4:32908491-32908510 None:intergenic 25.0%
! TTACTATAACCTACTTGAAA+GGG - chr1_4:32908509-32908528 Msa0160450:CDS 25.0%
!!! GATCTTTTATTGACTTTACA+AGG - chr1_4:32908841-32908860 Msa0160450:intron 25.0%
! AGAAGTTGTTAGAATAAAAG+AGG - chr1_4:32909343-32909362 Msa0160450:intron 25.0%
! GAAGTTGTTAGAATAAAAGA+GGG - chr1_4:32909344-32909363 Msa0160450:intron 25.0%
!!! ACAAGGTATACTTTGTTTTC+TGG - chr1_4:32907699-32907718 Msa0160450:three_prime_UTR 30.0%
! TCTTTGAAAGCAATTGACTT+TGG - chr1_4:32907908-32907927 Msa0160450:exon 30.0%
! CTGTCAATTTTCTTTAAACC+AGG - chr1_4:32907932-32907951 Msa0160450:CDS 30.0%
ACTAGTAAATAGCATTTACC+TGG + chr1_4:32907953-32907972 None:intergenic 30.0%
! CTTGTTCTTCATCATCATTT+TGG - chr1_4:32908005-32908024 Msa0160450:CDS 30.0%
TTTAGTTAACTGTGTATGGA+TGG + chr1_4:32908036-32908055 None:intergenic 30.0%
GAAAGATTTAACGAGATAGT+TGG - chr1_4:32908076-32908095 Msa0160450:intron 30.0%
TATCTTGCCAAAGCTATATT+TGG - chr1_4:32908227-32908246 Msa0160450:CDS 30.0%
TCAAAAGCCAAATATAGCTT+TGG + chr1_4:32908237-32908256 None:intergenic 30.0%
! TTGAACCTTTTATAAGTGAG+AGG - chr1_4:32908323-32908342 Msa0160450:intron 30.0%
GTTACTATAACCTACTTGAA+AGG - chr1_4:32908508-32908527 Msa0160450:CDS 30.0%
TTACCATGATCTTTCTTATG+TGG - chr1_4:32908555-32908574 Msa0160450:intron 30.0%
!!! TATTTTGACAAATGTGCTTG+TGG - chr1_4:32908619-32908638 Msa0160450:intron 30.0%
TGATGTCATGAATATTGTGA+TGG + chr1_4:32908723-32908742 None:intergenic 30.0%
ACACAAAAAAGCTTGAAAAC+TGG - chr1_4:32909015-32909034 Msa0160450:intron 30.0%
CACAAAAAAGCTTGAAAACT+GGG - chr1_4:32909016-32909035 Msa0160450:intron 30.0%
AAATCCTCCTAATGTTTCAT+TGG - chr1_4:32909096-32909115 Msa0160450:intron 30.0%
AATCCTCCTAATGTTTCATT+GGG - chr1_4:32909097-32909116 Msa0160450:intron 30.0%
!! CATTTACGACCCTTTTATTT+CGG - chr1_4:32909250-32909269 Msa0160450:CDS 30.0%
CTCACATAAAAGTCCAATAT+AGG + chr1_4:32909294-32909313 None:intergenic 30.0%
TCACATCTTCCATTAGTATT+TGG + chr1_4:32909465-32909484 None:intergenic 30.0%
TGAGTATATTCCCATTATCT+GGG - chr1_4:32909482-32909501 Msa0160450:intron 30.0%
!!! ACAAGGTATACTTTGTTTTC+TGG - chr1_4:32907699-32907718 Msa0160450:three_prime_UTR 30.0%
! TCTTTGAAAGCAATTGACTT+TGG - chr1_4:32907908-32907927 Msa0160450:exon 30.0%
! CTGTCAATTTTCTTTAAACC+AGG - chr1_4:32907932-32907951 Msa0160450:CDS 30.0%
ACTAGTAAATAGCATTTACC+TGG + chr1_4:32907953-32907972 None:intergenic 30.0%
! CTTGTTCTTCATCATCATTT+TGG - chr1_4:32908005-32908024 Msa0160450:CDS 30.0%
TTTAGTTAACTGTGTATGGA+TGG + chr1_4:32908036-32908055 None:intergenic 30.0%
GAAAGATTTAACGAGATAGT+TGG - chr1_4:32908076-32908095 Msa0160450:intron 30.0%
TATCTTGCCAAAGCTATATT+TGG - chr1_4:32908227-32908246 Msa0160450:CDS 30.0%
TCAAAAGCCAAATATAGCTT+TGG + chr1_4:32908237-32908256 None:intergenic 30.0%
! TTGAACCTTTTATAAGTGAG+AGG - chr1_4:32908323-32908342 Msa0160450:intron 30.0%
GTTACTATAACCTACTTGAA+AGG - chr1_4:32908508-32908527 Msa0160450:CDS 30.0%
TTACCATGATCTTTCTTATG+TGG - chr1_4:32908555-32908574 Msa0160450:intron 30.0%
!!! TATTTTGACAAATGTGCTTG+TGG - chr1_4:32908619-32908638 Msa0160450:intron 30.0%
TGATGTCATGAATATTGTGA+TGG + chr1_4:32908723-32908742 None:intergenic 30.0%
ACACAAAAAAGCTTGAAAAC+TGG - chr1_4:32909015-32909034 Msa0160450:intron 30.0%
CACAAAAAAGCTTGAAAACT+GGG - chr1_4:32909016-32909035 Msa0160450:intron 30.0%
AAATCCTCCTAATGTTTCAT+TGG - chr1_4:32909096-32909115 Msa0160450:intron 30.0%
AATCCTCCTAATGTTTCATT+GGG - chr1_4:32909097-32909116 Msa0160450:intron 30.0%
!! CATTTACGACCCTTTTATTT+CGG - chr1_4:32909250-32909269 Msa0160450:CDS 30.0%
CTCACATAAAAGTCCAATAT+AGG + chr1_4:32909294-32909313 None:intergenic 30.0%
TCACATCTTCCATTAGTATT+TGG + chr1_4:32909465-32909484 None:intergenic 30.0%
TGAGTATATTCCCATTATCT+GGG - chr1_4:32909482-32909501 Msa0160450:intron 30.0%
GGAGTTAAAATGTTAGGACA+AGG - chr1_4:32907682-32907701 Msa0160450:three_prime_UTR 35.0%
! GTGTAAATTTTCCCTTCTGT+TGG - chr1_4:32907763-32907782 Msa0160450:three_prime_UTR 35.0%
! TTTTAGATGTGCCACAAACA+TGG - chr1_4:32907824-32907843 Msa0160450:three_prime_UTR 35.0%
TCTGCAAACAAAAAGTTCTC+GGG + chr1_4:32907872-32907891 None:intergenic 35.0%
GTCTGCAAACAAAAAGTTCT+CGG + chr1_4:32907873-32907892 None:intergenic 35.0%
!! CTTTTTGTTTGCAGACAAGA+AGG - chr1_4:32907877-32907896 Msa0160450:three_prime_UTR 35.0%
AGTTGGAAGTCCATATTACA+TGG - chr1_4:32908093-32908112 Msa0160450:intron 35.0%
CACTCCAGATATCAATTTCA+GGG + chr1_4:32908147-32908166 None:intergenic 35.0%
!! TTTCTGGTGTCAATCCTTTT+TGG - chr1_4:32908191-32908210 Msa0160450:CDS 35.0%
!! TTCTGGTGTCAATCCTTTTT+GGG - chr1_4:32908192-32908211 Msa0160450:CDS 35.0%
!!! GCTATATTTGGCTTTTGAGT+CGG - chr1_4:32908239-32908258 Msa0160450:CDS 35.0%
! TGTATCCTAGATGTGGATTT+TGG + chr1_4:32908282-32908301 None:intergenic 35.0%
! AAAATCCACATCTAGGATAC+AGG - chr1_4:32908281-32908300 Msa0160450:intron 35.0%
! TCTTAAGGCGTTAGGTTTAT+GGG + chr1_4:32908350-32908369 None:intergenic 35.0%
! TTCTTAAGGCGTTAGGTTTA+TGG + chr1_4:32908351-32908370 None:intergenic 35.0%
ACCTAACGCCTTAAGAATTT+AGG - chr1_4:32908354-32908373 Msa0160450:intron 35.0%
! ACCTAAATTCTTAAGGCGTT+AGG + chr1_4:32908358-32908377 None:intergenic 35.0%
AGAAACCATACAAGTGAGTT+GGG + chr1_4:32908393-32908412 None:intergenic 35.0%
AAAATATGTGCGCCTCTATT+AGG + chr1_4:32908456-32908475 None:intergenic 35.0%
! ATTACAACACCCTTTCAAGT+AGG + chr1_4:32908521-32908540 None:intergenic 35.0%
CATCCACATAAGAAAGATCA+TGG + chr1_4:32908561-32908580 None:intergenic 35.0%
CTTTCTTATGTGGATGAAGA+TGG - chr1_4:32908565-32908584 Msa0160450:intron 35.0%
!! AGATGGTATTTTTGTGCTTG+TGG - chr1_4:32908582-32908601 Msa0160450:intron 35.0%
TTAAAATGCAGAAACTGAGC+AGG - chr1_4:32908797-32908816 Msa0160450:intron 35.0%
TAAAATGCAGAAACTGAGCA+GGG - chr1_4:32908798-32908817 Msa0160450:intron 35.0%
TTCGCATTATCAGAAACCTT+TGG + chr1_4:32908876-32908895 None:intergenic 35.0%
GATCAAGCATCTTCTTAACA+AGG + chr1_4:32908902-32908921 None:intergenic 35.0%
AACTAAATGCAGATCATCCA+TGG - chr1_4:32909058-32909077 Msa0160450:intron 35.0%
GTTTCTCCCAATGAAACATT+AGG + chr1_4:32909106-32909125 None:intergenic 35.0%
AACTTAAGAAAACAGCACTG+AGG - chr1_4:32909168-32909187 Msa0160450:CDS 35.0%
! ACTTAAGAAAACAGCACTGA+GGG - chr1_4:32909169-32909188 Msa0160450:CDS 35.0%
CGAAATAAAAGGGTCGTAAA+TGG + chr1_4:32909252-32909271 None:intergenic 35.0%
! TATTGGACTTTTATGTGAGC+AGG - chr1_4:32909295-32909314 Msa0160450:intron 35.0%
AACATTGATGAACTACGAGT+AGG - chr1_4:32909404-32909423 Msa0160450:intron 35.0%
ACATTGATGAACTACGAGTA+GGG - chr1_4:32909405-32909424 Msa0160450:intron 35.0%
TGGAGATGTCCAAATACTAA+TGG - chr1_4:32909453-32909472 Msa0160450:intron 35.0%
GTGAGTATATTCCCATTATC+TGG - chr1_4:32909481-32909500 Msa0160450:intron 35.0%
CAGAATAGATCCCAGATAAT+GGG + chr1_4:32909495-32909514 None:intergenic 35.0%
ACAGAATAGATCCCAGATAA+TGG + chr1_4:32909496-32909515 None:intergenic 35.0%
TACTCATGTTGACAGTTAGT+TGG + chr1_4:32909558-32909577 None:intergenic 35.0%
GATGGATGACTTTCTATTAC+CGG + chr1_4:32909609-32909628 None:intergenic 35.0%
GTAATAGAAAGTCATCCATC+TGG - chr1_4:32909609-32909628 Msa0160450:intron 35.0%
TGCTTATATTCAAGACCAGA+TGG + chr1_4:32909627-32909646 None:intergenic 35.0%
GGAGTTAAAATGTTAGGACA+AGG - chr1_4:32907682-32907701 Msa0160450:three_prime_UTR 35.0%
! GTGTAAATTTTCCCTTCTGT+TGG - chr1_4:32907763-32907782 Msa0160450:three_prime_UTR 35.0%
! TTTTAGATGTGCCACAAACA+TGG - chr1_4:32907824-32907843 Msa0160450:three_prime_UTR 35.0%
TCTGCAAACAAAAAGTTCTC+GGG + chr1_4:32907872-32907891 None:intergenic 35.0%
GTCTGCAAACAAAAAGTTCT+CGG + chr1_4:32907873-32907892 None:intergenic 35.0%
!! CTTTTTGTTTGCAGACAAGA+AGG - chr1_4:32907877-32907896 Msa0160450:three_prime_UTR 35.0%
AGTTGGAAGTCCATATTACA+TGG - chr1_4:32908093-32908112 Msa0160450:intron 35.0%
CACTCCAGATATCAATTTCA+GGG + chr1_4:32908147-32908166 None:intergenic 35.0%
!! TTTCTGGTGTCAATCCTTTT+TGG - chr1_4:32908191-32908210 Msa0160450:CDS 35.0%
!! TTCTGGTGTCAATCCTTTTT+GGG - chr1_4:32908192-32908211 Msa0160450:CDS 35.0%
!!! GCTATATTTGGCTTTTGAGT+CGG - chr1_4:32908239-32908258 Msa0160450:CDS 35.0%
! TGTATCCTAGATGTGGATTT+TGG + chr1_4:32908282-32908301 None:intergenic 35.0%
! AAAATCCACATCTAGGATAC+AGG - chr1_4:32908281-32908300 Msa0160450:intron 35.0%
! TCTTAAGGCGTTAGGTTTAT+GGG + chr1_4:32908350-32908369 None:intergenic 35.0%
! TTCTTAAGGCGTTAGGTTTA+TGG + chr1_4:32908351-32908370 None:intergenic 35.0%
ACCTAACGCCTTAAGAATTT+AGG - chr1_4:32908354-32908373 Msa0160450:intron 35.0%
! ACCTAAATTCTTAAGGCGTT+AGG + chr1_4:32908358-32908377 None:intergenic 35.0%
AGAAACCATACAAGTGAGTT+GGG + chr1_4:32908393-32908412 None:intergenic 35.0%
AAAATATGTGCGCCTCTATT+AGG + chr1_4:32908456-32908475 None:intergenic 35.0%
! ATTACAACACCCTTTCAAGT+AGG + chr1_4:32908521-32908540 None:intergenic 35.0%
CATCCACATAAGAAAGATCA+TGG + chr1_4:32908561-32908580 None:intergenic 35.0%
CTTTCTTATGTGGATGAAGA+TGG - chr1_4:32908565-32908584 Msa0160450:intron 35.0%
!! AGATGGTATTTTTGTGCTTG+TGG - chr1_4:32908582-32908601 Msa0160450:intron 35.0%
TTAAAATGCAGAAACTGAGC+AGG - chr1_4:32908797-32908816 Msa0160450:intron 35.0%
TAAAATGCAGAAACTGAGCA+GGG - chr1_4:32908798-32908817 Msa0160450:intron 35.0%
TTCGCATTATCAGAAACCTT+TGG + chr1_4:32908876-32908895 None:intergenic 35.0%
GATCAAGCATCTTCTTAACA+AGG + chr1_4:32908902-32908921 None:intergenic 35.0%
AACTAAATGCAGATCATCCA+TGG - chr1_4:32909058-32909077 Msa0160450:intron 35.0%
GTTTCTCCCAATGAAACATT+AGG + chr1_4:32909106-32909125 None:intergenic 35.0%
AACTTAAGAAAACAGCACTG+AGG - chr1_4:32909168-32909187 Msa0160450:CDS 35.0%
! ACTTAAGAAAACAGCACTGA+GGG - chr1_4:32909169-32909188 Msa0160450:CDS 35.0%
CGAAATAAAAGGGTCGTAAA+TGG + chr1_4:32909252-32909271 None:intergenic 35.0%
! TATTGGACTTTTATGTGAGC+AGG - chr1_4:32909295-32909314 Msa0160450:intron 35.0%
AACATTGATGAACTACGAGT+AGG - chr1_4:32909404-32909423 Msa0160450:intron 35.0%
ACATTGATGAACTACGAGTA+GGG - chr1_4:32909405-32909424 Msa0160450:intron 35.0%
TGGAGATGTCCAAATACTAA+TGG - chr1_4:32909453-32909472 Msa0160450:intron 35.0%
GTGAGTATATTCCCATTATC+TGG - chr1_4:32909481-32909500 Msa0160450:intron 35.0%
CAGAATAGATCCCAGATAAT+GGG + chr1_4:32909495-32909514 None:intergenic 35.0%
ACAGAATAGATCCCAGATAA+TGG + chr1_4:32909496-32909515 None:intergenic 35.0%
TACTCATGTTGACAGTTAGT+TGG + chr1_4:32909558-32909577 None:intergenic 35.0%
GATGGATGACTTTCTATTAC+CGG + chr1_4:32909609-32909628 None:intergenic 35.0%
GTAATAGAAAGTCATCCATC+TGG - chr1_4:32909609-32909628 Msa0160450:intron 35.0%
TGCTTATATTCAAGACCAGA+TGG + chr1_4:32909627-32909646 None:intergenic 35.0%
!!! GGTATACTTTGTTTTCTGGC+TGG - chr1_4:32907703-32907722 Msa0160450:three_prime_UTR 40.0%
ACAAACATGGTGTGATGCAT+CGG - chr1_4:32907837-32907856 Msa0160450:three_prime_UTR 40.0%
ATGGCCCTGAAATTGATATC+TGG - chr1_4:32908140-32908159 Msa0160450:intron 40.0%
GCACTCCAGATATCAATTTC+AGG + chr1_4:32908148-32908167 None:intergenic 40.0%
! GAAATTGATATCTGGAGTGC+TGG - chr1_4:32908148-32908167 Msa0160450:intron 40.0%
AAAAACCAACCTGCCCAAAA+AGG + chr1_4:32908208-32908227 None:intergenic 40.0%
ACCAACCAAAATCCACATCT+AGG - chr1_4:32908274-32908293 Msa0160450:intron 40.0%
!! TCCTAGATGTGGATTTTGGT+TGG + chr1_4:32908278-32908297 None:intergenic 40.0%
CATAACCTGTATCCTAGATG+TGG + chr1_4:32908289-32908308 None:intergenic 40.0%
AGGATACAGGTTATGTAGCA+AGG - chr1_4:32908294-32908313 Msa0160450:intron 40.0%
GGATACAGGTTATGTAGCAA+GGG - chr1_4:32908295-32908314 Msa0160450:intron 40.0%
TGGGTCCTCTCACTTATAAA+AGG + chr1_4:32908331-32908350 None:intergenic 40.0%
GAGAAACCATACAAGTGAGT+TGG + chr1_4:32908394-32908413 None:intergenic 40.0%
TTGACTTTACAAGGGTTCCA+TGG - chr1_4:32908850-32908869 Msa0160450:intron 40.0%
TTATCAGAAACCTTTGGCCA+TGG + chr1_4:32908870-32908889 None:intergenic 40.0%
TTCTTTGCATTCTGCAACCA+TGG + chr1_4:32909078-32909097 None:intergenic 40.0%
TCTCCCAATGAAACATTAGG+AGG + chr1_4:32909103-32909122 None:intergenic 40.0%
AGTATTTGGACATCTCCATC+AGG + chr1_4:32909451-32909470 None:intergenic 40.0%
!!! GGTATACTTTGTTTTCTGGC+TGG - chr1_4:32907703-32907722 Msa0160450:three_prime_UTR 40.0%
ACAAACATGGTGTGATGCAT+CGG - chr1_4:32907837-32907856 Msa0160450:three_prime_UTR 40.0%
ATGGCCCTGAAATTGATATC+TGG - chr1_4:32908140-32908159 Msa0160450:intron 40.0%
GCACTCCAGATATCAATTTC+AGG + chr1_4:32908148-32908167 None:intergenic 40.0%
! GAAATTGATATCTGGAGTGC+TGG - chr1_4:32908148-32908167 Msa0160450:intron 40.0%
AAAAACCAACCTGCCCAAAA+AGG + chr1_4:32908208-32908227 None:intergenic 40.0%
ACCAACCAAAATCCACATCT+AGG - chr1_4:32908274-32908293 Msa0160450:intron 40.0%
!! TCCTAGATGTGGATTTTGGT+TGG + chr1_4:32908278-32908297 None:intergenic 40.0%
CATAACCTGTATCCTAGATG+TGG + chr1_4:32908289-32908308 None:intergenic 40.0%
AGGATACAGGTTATGTAGCA+AGG - chr1_4:32908294-32908313 Msa0160450:intron 40.0%
GGATACAGGTTATGTAGCAA+GGG - chr1_4:32908295-32908314 Msa0160450:intron 40.0%
TGGGTCCTCTCACTTATAAA+AGG + chr1_4:32908331-32908350 None:intergenic 40.0%
GAGAAACCATACAAGTGAGT+TGG + chr1_4:32908394-32908413 None:intergenic 40.0%
TTGACTTTACAAGGGTTCCA+TGG - chr1_4:32908850-32908869 Msa0160450:intron 40.0%
TTATCAGAAACCTTTGGCCA+TGG + chr1_4:32908870-32908889 None:intergenic 40.0%
TTCTTTGCATTCTGCAACCA+TGG + chr1_4:32909078-32909097 None:intergenic 40.0%
TCTCCCAATGAAACATTAGG+AGG + chr1_4:32909103-32909122 None:intergenic 40.0%
AGTATTTGGACATCTCCATC+AGG + chr1_4:32909451-32909470 None:intergenic 40.0%
GATGCATCACACCATGTTTG+TGG + chr1_4:32907838-32907857 None:intergenic 45.0%
CAAACATGGTGTGATGCATC+GGG - chr1_4:32907838-32907857 Msa0160450:three_prime_UTR 45.0%
GTTAACTGTGTATGGATGGC+AGG + chr1_4:32908032-32908051 None:intergenic 45.0%
ACTTCAGGGGCCATGTAATA+TGG + chr1_4:32908106-32908125 None:intergenic 45.0%
!! TCAATCCTTTTTGGGCAGGT+TGG - chr1_4:32908200-32908219 Msa0160450:CDS 45.0%
TGATGCCCAACTCACTTGTA+TGG - chr1_4:32908385-32908404 Msa0160450:intron 45.0%
!! CTTACTGCACAGGAAGTGTT+AGG - chr1_4:32908939-32908958 Msa0160450:intron 45.0%
GAAAATTGTGTGAGCCTTGC+TGG + chr1_4:32909137-32909156 None:intergenic 45.0%
ACTACGAGTAGGGTTAAGCT+AGG - chr1_4:32909415-32909434 Msa0160450:intron 45.0%
GGACATCTCCATCAGGAATT+CGG + chr1_4:32909444-32909463 None:intergenic 45.0%
TCTGTTTGTTCCACAGTCGA+TGG - chr1_4:32909511-32909530 Msa0160450:intron 45.0%
! ATTTCCACATCCATCGACTG+TGG + chr1_4:32909524-32909543 None:intergenic 45.0%
GATGCATCACACCATGTTTG+TGG + chr1_4:32907838-32907857 None:intergenic 45.0%
CAAACATGGTGTGATGCATC+GGG - chr1_4:32907838-32907857 Msa0160450:three_prime_UTR 45.0%
GTTAACTGTGTATGGATGGC+AGG + chr1_4:32908032-32908051 None:intergenic 45.0%
ACTTCAGGGGCCATGTAATA+TGG + chr1_4:32908106-32908125 None:intergenic 45.0%
!! TCAATCCTTTTTGGGCAGGT+TGG - chr1_4:32908200-32908219 Msa0160450:CDS 45.0%
TGATGCCCAACTCACTTGTA+TGG - chr1_4:32908385-32908404 Msa0160450:intron 45.0%
!! CTTACTGCACAGGAAGTGTT+AGG - chr1_4:32908939-32908958 Msa0160450:intron 45.0%
GAAAATTGTGTGAGCCTTGC+TGG + chr1_4:32909137-32909156 None:intergenic 45.0%
ACTACGAGTAGGGTTAAGCT+AGG - chr1_4:32909415-32909434 Msa0160450:intron 45.0%
GGACATCTCCATCAGGAATT+CGG + chr1_4:32909444-32909463 None:intergenic 45.0%
TCTGTTTGTTCCACAGTCGA+TGG - chr1_4:32909511-32909530 Msa0160450:intron 45.0%
! ATTTCCACATCCATCGACTG+TGG + chr1_4:32909524-32909543 None:intergenic 45.0%
!! GGTGTCAATCCTTTTTGGGC+AGG - chr1_4:32908196-32908215 Msa0160450:CDS 50.0%
CAGGTTATGTAGCAAGGGTG+AGG - chr1_4:32908300-32908319 Msa0160450:intron 50.0%
TGGTTTCTCTGAGCCCAATG+TGG - chr1_4:32908405-32908424 Msa0160450:CDS 50.0%
CTCTATTAGGTCACGAGGAG+AGG + chr1_4:32908443-32908462 None:intergenic 50.0%
CTCTCCTCGTGACCTAATAG+AGG - chr1_4:32908441-32908460 Msa0160450:CDS 50.0%
TACAAGGGTTCCATGGCCAA+AGG - chr1_4:32908857-32908876 Msa0160450:intron 50.0%
ACACTTCCTGTGCAGTAAGC+CGG + chr1_4:32908938-32908957 None:intergenic 50.0%
CTAGGTCACCGAATTCCTGA+TGG - chr1_4:32909433-32909452 Msa0160450:intron 50.0%
TGTTCCACAGTCGATGGATG+TGG - chr1_4:32909517-32909536 Msa0160450:intron 50.0%
!! GGTGTCAATCCTTTTTGGGC+AGG - chr1_4:32908196-32908215 Msa0160450:CDS 50.0%
CAGGTTATGTAGCAAGGGTG+AGG - chr1_4:32908300-32908319 Msa0160450:intron 50.0%
TGGTTTCTCTGAGCCCAATG+TGG - chr1_4:32908405-32908424 Msa0160450:CDS 50.0%
CTCTATTAGGTCACGAGGAG+AGG + chr1_4:32908443-32908462 None:intergenic 50.0%
CTCTCCTCGTGACCTAATAG+AGG - chr1_4:32908441-32908460 Msa0160450:CDS 50.0%
TACAAGGGTTCCATGGCCAA+AGG - chr1_4:32908857-32908876 Msa0160450:intron 50.0%
ACACTTCCTGTGCAGTAAGC+CGG + chr1_4:32908938-32908957 None:intergenic 50.0%
CTAGGTCACCGAATTCCTGA+TGG - chr1_4:32909433-32909452 Msa0160450:intron 50.0%
TGTTCCACAGTCGATGGATG+TGG - chr1_4:32909517-32909536 Msa0160450:intron 50.0%
GTCAGGGGATCGTCCACATT+GGG + chr1_4:32908421-32908440 None:intergenic 55.0%
TAGGTCACGAGGAGAGGTCA+GGG + chr1_4:32908437-32908456 None:intergenic 55.0%
TTAGGTCACGAGGAGAGGTC+AGG + chr1_4:32908438-32908457 None:intergenic 55.0%
TGCGCCTCTATTAGGTCACG+AGG + chr1_4:32908448-32908467 None:intergenic 55.0%
TTGATCCTGACCCAAAGCGC+CGG - chr1_4:32908916-32908935 Msa0160450:intron 55.0%
AGAAACAGTCCGAGCCAGCA+AGG - chr1_4:32909120-32909139 Msa0160450:intron 55.0%
! TTGTGTGAGCCTTGCTGGCT+CGG + chr1_4:32909132-32909151 None:intergenic 55.0%
GTCAGGGGATCGTCCACATT+GGG + chr1_4:32908421-32908440 None:intergenic 55.0%
TAGGTCACGAGGAGAGGTCA+GGG + chr1_4:32908437-32908456 None:intergenic 55.0%
TTAGGTCACGAGGAGAGGTC+AGG + chr1_4:32908438-32908457 None:intergenic 55.0%
TGCGCCTCTATTAGGTCACG+AGG + chr1_4:32908448-32908467 None:intergenic 55.0%
TTGATCCTGACCCAAAGCGC+CGG - chr1_4:32908916-32908935 Msa0160450:intron 55.0%
AGAAACAGTCCGAGCCAGCA+AGG - chr1_4:32909120-32909139 Msa0160450:intron 55.0%
! TTGTGTGAGCCTTGCTGGCT+CGG + chr1_4:32909132-32909151 None:intergenic 55.0%
GGTCAGGGGATCGTCCACAT+TGG + chr1_4:32908422-32908441 None:intergenic 60.0%
AGGTCACGAGGAGAGGTCAG+GGG + chr1_4:32908436-32908455 None:intergenic 60.0%
GTGCAGTAAGCCGGCGCTTT+GGG + chr1_4:32908929-32908948 None:intergenic 60.0%
TGTGCAGTAAGCCGGCGCTT+TGG + chr1_4:32908930-32908949 None:intergenic 60.0%
!! AAAGCGCCGGCTTACTGCAC+AGG - chr1_4:32908929-32908948 Msa0160450:intron 60.0%
GGTCAGGGGATCGTCCACAT+TGG + chr1_4:32908422-32908441 None:intergenic 60.0%
AGGTCACGAGGAGAGGTCAG+GGG + chr1_4:32908436-32908455 None:intergenic 60.0%
GTGCAGTAAGCCGGCGCTTT+GGG + chr1_4:32908929-32908948 None:intergenic 60.0%
TGTGCAGTAAGCCGGCGCTT+TGG + chr1_4:32908930-32908949 None:intergenic 60.0%
!! AAAGCGCCGGCTTACTGCAC+AGG - chr1_4:32908929-32908948 Msa0160450:intron 60.0%
! GTAAGCCGGCGCTTTGGGTC+AGG + chr1_4:32908924-32908943 None:intergenic 65.0%
! GTAAGCCGGCGCTTTGGGTC+AGG + chr1_4:32908924-32908943 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 32907670 32909689 32907670 ID=Msa0160450;Name=Msa0160450
chr1_4 mRNA 32907670 32909689 32907670 ID=Msa0160450-mRNA-1;Parent=Msa0160450;Name=Msa0160450-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|0.25|0.2|0.8|0.5|0.4|5|253|188
chr1_4 exon 32907670 32908042 32907670 ID=Msa0160450-mRNA-1:exon:10381;Parent=Msa0160450-mRNA-1
chr1_4 exon 32908169 32908289 32908169 ID=Msa0160450-mRNA-1:exon:10380;Parent=Msa0160450-mRNA-1
chr1_4 exon 32908404 32908551 32908404 ID=Msa0160450-mRNA-1:exon:10379;Parent=Msa0160450-mRNA-1
chr1_4 exon 32909142 32909285 32909142 ID=Msa0160450-mRNA-1:exon:10378;Parent=Msa0160450-mRNA-1
chr1_4 exon 32909656 32909689 32909656 ID=Msa0160450-mRNA-1:exon:10377;Parent=Msa0160450-mRNA-1
chr1_4 CDS 32909656 32909689 32909656 ID=Msa0160450-mRNA-1:cds;Parent=Msa0160450-mRNA-1
chr1_4 CDS 32909142 32909285 32909142 ID=Msa0160450-mRNA-1:cds;Parent=Msa0160450-mRNA-1
chr1_4 CDS 32908404 32908551 32908404 ID=Msa0160450-mRNA-1:cds;Parent=Msa0160450-mRNA-1
chr1_4 CDS 32908169 32908289 32908169 ID=Msa0160450-mRNA-1:cds;Parent=Msa0160450-mRNA-1
chr1_4 CDS 32907923 32908042 32907923 ID=Msa0160450-mRNA-1:cds;Parent=Msa0160450-mRNA-1
chr1_4 three_prime_UTR 32907670 32907922 32907670 ID=Msa0160450-mRNA-1:three_prime_utr;Parent=Msa0160450-mRNA-1
Gene Sequence

>Msa0160450

ATGTTAACATTTGGAGTTAAAATGTTAGGACAAGGTGAAAGATTTAACGAGATAGTTGGAAGTCCATATTACATGGCCCCTGAAGTATTAAAAAGAAATTATGGCCCTGAAATTGATATCTGGAGTGCTGGAGTTATTCTTTACATCTTACTTTCTGGTGTCAATCCTTTTTGGGCAGAAACTGAGCAGGGAGTTGCTCAAGCAATTTTAAGATCTTTTATTGACTTTACAAGGGTTCCATGGCCAAAGGTTTCTGATAATGCGAAAGACCTTGTTAAGAAGATGCTTGATCCTGACCCAAAGCGCCGGCTTACTGCACAGGAAGTATCATCCATGGTTGCAGAATGCAAAGAAATCCTCCTAATGTTTCATTGGGAGAAACAGTCCGAGCCAGCAAGGCTCACACAATTTTCTGTAATGAACAAACTTAAGAAAACAGCACTGAGGGTGATTGCAGAGCATTTATCTGTAGAAGAAGTTGTTAGAATAAAAGAGGGATTCCGAGTGATATATACAGAAAATAAAGGCAAAATCAACATTGATGAACTACGAGTAGGGTTAAGCTAG
Protein sequence

>Msa0160450

MLTFGVKMLGQGERFNEIVGSPYYMAPEVLKRNYGPEIDIWSAGVILYILLSGVNPFWAETEQGVAQAILRSFIDFTRVPWPKVSDNAKDLVKKMLDPDPKRRLTAQEVSSMVAECKEILLMFHWEKQSEPARLTQFSVMNKLKKTALRVIAEHLSVEEVVRIKEGFRVIYTENKGKINIDELRVGLS*