AlfalfaGEDB | Msa0165400

M. sativa cultivar zhongmu-4 / Msa0165400

KEGG: -
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009889 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010072 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0022622 GO:0023052 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048364 GO:0048367 GO:0048507 GO:0048508 GO:0048527 GO:0048528 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0080090 GO:0090421 GO:0090696 GO:0099402 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0165400	QDC33492.1	99.636	275	1	0	1	275	1	275	0.0	582

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0165400	sp\|Q9S851\|NAC31_ARATH	57.500	160	65	1	9	165	21	180	1.34e-61	200

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0165400	A0A4Y5UM74	99.636	275	1	0	1	275	1	275	0.0	582

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TFs/TRs:

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Gene ID	Type	Classification
Msa0165400	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0027490	Msa0165400	0.986346	1.757506e-166	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0165400	MtrunA17_Chr1g0183741	98.909	275	3	0	1	275	1	275	0.0	579
Msa0165400	MtrunA17_Chr7g0261411	76.109	293	50	7	1	275	1	291	4.91e-152	426
Msa0165400	MtrunA17_Chr2g0312321	46.914	243	111	5	6	234	7	245	2.00e-67	211
Msa0165400	MtrunA17_Chr2g0316971	56.250	160	64	1	8	161	21	180	4.17e-61	197
Msa0165400	MtrunA17_Chr3g0139331	43.333	240	110	5	9	234	10	237	1.63e-59	192
Msa0165400	MtrunA17_Chr4g0060931	48.691	191	82	4	10	186	17	205	5.67e-59	191
Msa0165400	MtrunA17_Chr1g0147421	53.797	158	70	2	2	156	19	176	1.38e-58	190
Msa0165400	MtrunA17_Chr4g0018391	45.813	203	91	5	8	194	5	204	1.97e-57	187
Msa0165400	MtrunA17_Chr3g0091921	49.733	187	78	5	10	182	16	200	9.99e-57	184
Msa0165400	MtrunA17_Chr5g0405211	48.205	195	89	5	8	192	6	198	1.93e-56	184

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0165400	AT4G28530.1	72.414	174	32	2	1	158	1	174	2.35e-89	269
Msa0165400	AT1G76420.1	57.500	160	65	1	9	165	21	180	1.36e-62	200
Msa0165400	AT1G56010.2	43.011	279	137	8	6	274	15	281	1.58e-61	197
Msa0165400	AT2G24430.2	56.291	151	63	1	9	156	15	165	3.22e-61	196
Msa0165400	AT2G24430.1	56.291	151	63	1	9	156	15	165	3.22e-61	196
Msa0165400	AT5G53950.1	50.276	181	84	1	10	184	17	197	1.04e-59	194
Msa0165400	AT2G18060.3	50.265	189	83	6	8	187	7	193	1.28e-59	194
Msa0165400	AT2G18060.1	50.265	189	83	6	8	187	7	193	1.28e-59	194
Msa0165400	AT5G07680.2	57.516	153	57	3	10	156	3	153	1.23e-58	189
Msa0165400	AT3G12977.1	56.410	156	61	3	6	156	14	167	1.26e-58	188

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Find 103 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGATGAGAAAGAAGCAAAT+TGG	0.341217	1_4:-45886426	None:intergenic
ACAAATGAGGATGTTCTCAA+GGG	0.646017	1_4:+45883273	Msa0165400:CDS
ACACATTGATTAGCATTTGT+AGG	0.419765	1_4:+45886899	Msa0165400:three_prime_UTR
ACATTGATTAGCATTTGTAG+GGG	0.662335	1_4:+45886901	Msa0165400:three_prime_UTR
ACCAACCAAACCATGCCAAT+TGG	0.386891	1_4:+45886397	Msa0165400:CDS
ACCAATAGGGCAACAACATC+TGG	0.310609	1_4:+45885276	Msa0165400:CDS
ACGTGCATGATTGGGATTAT+AGG	0.305437	1_4:+45886804	Msa0165400:three_prime_UTR
ACTTGCATACATGCGAGCCT+TGG	0.522819	1_4:+45883313	Msa0165400:CDS
AGCAACAGGAAAGGATCGAA+CGG	0.629108	1_4:+45885308	Msa0165400:CDS
AGGACCAATTCTTCATCACT+AGG	0.581995	1_4:-45883228	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTATCCTAGTGATGAAGAAT+TGG	+	chr1_4:45883224-45883243	Msa0165400:CDS	30.0%
AATTGACATTCACAAACACA+TGG	+	chr1_4:45883456-45883475	Msa0165400:intron	30.0%
TACGGCATCATTAATTAACT+TGG	+	chr1_4:45883481-45883500	Msa0165400:intron	30.0%
GTTCAGTTCTTCAATGATTA+AGG	+	chr1_4:45883749-45883768	Msa0165400:intron	30.0%
TACAGCAGAAAAAAACAACT+AGG	-	chr1_4:45883898-45883917	None:intergenic	30.0%
GTGAAAGATCAAATGTTGTA+TGG	-	chr1_4:45884195-45884214	None:intergenic	30.0%
AGAAAGTCGTCATACAAAAT+AGG	+	chr1_4:45884363-45884382	Msa0165400:intron	30.0%
TATGGAAAAACATGTGACTT+AGG	-	chr1_4:45885112-45885131	None:intergenic	30.0%
CATGCATGAATAGAGATAAT+AGG	-	chr1_4:45885151-45885170	None:intergenic	30.0%
TAAAACCTGTGGCATATTTA+CGG	-	chr1_4:45885254-45885273	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	45883026	45886962	45883026	ID=Msa0165400;Name=Msa0165400
chr1_4	mRNA	45883026	45886962	45883026	ID=Msa0165400-mRNA-1;Parent=Msa0165400;Name=Msa0165400-mRNA-1;_AED=0.02;_eAED=0.02;_QI=151\|1\|1\|1\|1\|1\|3\|272\|275
chr1_4	exon	45883026	45883345	45883026	ID=Msa0165400-mRNA-1:exon:13131;Parent=Msa0165400-mRNA-1
chr1_4	exon	45885202	45885467	45885202	ID=Msa0165400-mRNA-1:exon:13132;Parent=Msa0165400-mRNA-1
chr1_4	exon	45886298	45886962	45886298	ID=Msa0165400-mRNA-1:exon:13133;Parent=Msa0165400-mRNA-1
chr1_4	five_prime_UTR	45883026	45883176	45883026	ID=Msa0165400-mRNA-1:five_prime_utr;Parent=Msa0165400-mRNA-1
chr1_4	CDS	45883177	45883345	45883177	ID=Msa0165400-mRNA-1:cds;Parent=Msa0165400-mRNA-1
chr1_4	CDS	45885202	45885467	45885202	ID=Msa0165400-mRNA-1:cds;Parent=Msa0165400-mRNA-1
chr1_4	CDS	45886298	45886690	45886298	ID=Msa0165400-mRNA-1:cds;Parent=Msa0165400-mRNA-1
chr1_4	three_prime_UTR	45886691	45886962	45886691	ID=Msa0165400-mRNA-1:three_prime_utr;Parent=Msa0165400-mRNA-1

Gene Sequence

>Msa0165400

ATGGGTCTTAGAGATGTCGGTGCTTCACTTCCCCCTGGCTTTCGATTTTATCCTAGTGATGAAGAATTGGTCCTTCATTACCTTTACAAAAAAATCACAAATGAGGATGTTCTCAAGGGTACCTTGGAGGAAATTGACTTGCATACATGCGAGCCTTGGCAACTTCCTGAGGTGGCTAAACTCAACGCAACTGAATGGTACTTCTTCAGCTTTCGTGACCGTAAATATGCCACAGGTTTTAGAACCAATAGGGCAACAACATCTGGGTATTGGAAAGCAACAGGAAAGGATCGAACGGTGTTTGATCCAATCACACATGAGGTTGTAGGAATGAGGAAAACTCTAGTGTTCTATAGGAATAGAGCTCCAAATGGAATCAAAACAGGTTGGATCATGCATGAGTTTAGGTTGGAGACCCCACATATGCCACCTAAGGAAGACTGGGTTTTGTGTAGAGTATTTCATAAAGGCAAGACAGAAAATAATGCCAAATTAAGTCCACAAGAAATGTATGAGACTATAGCCCCATCCCTAACCAACCAAACCATGCCAATTGGGTACAACCAATTTGCTTCTTTCTCATCTTCAATGACAACCCACCATTGTCATTTAAATGAGAACCAAAATAATTCTTTGATGAATCTTCTTCAATTTTCTAAGGAAACAAACAACAATTGTAGTAGTGTTACTCAAATTAGTCCTAAATGTGATGATGGATATGGGTTTTTGTGGGACATGGATTTGGAAGATCATCATGATGGTGTGGCCTCATCAGACATGGAAGGAATGAGATTCGAGGTTGATAATAATAGCATGGTCTTGTTATAA

Protein sequence

>Msa0165400

MGLRDVGASLPPGFRFYPSDEELVLHYLYKKITNEDVLKGTLEEIDLHTCEPWQLPEVAKLNATEWYFFSFRDRKYATGFRTNRATTSGYWKATGKDRTVFDPITHEVVGMRKTLVFYRNRAPNGIKTGWIMHEFRLETPHMPPKEDWVLCRVFHKGKTENNAKLSPQEMYETIAPSLTNQTMPIGYNQFASFSSSMTTHHCHLNENQNNSLMNLLQFSKETNNNCSSVTQISPKCDDGYGFLWDMDLEDHHDGVASSDMEGMRFEVDNNSMVLL*