AlfalfaGEDB | Msa0165770

M. sativa cultivar zhongmu-4 / Msa0165770

KEGG: K01426
GO: GO:0003674 GO:0003824 GO:0004040 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006082 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009683 GO:0009684 GO:0009850 GO:0009851 GO:0009987 GO:0010817 GO:0016053 GO:0016787 GO:0016810 GO:0016811 GO:0018130 GO:0019438 GO:0019752 GO:0031974 GO:0031981 GO:0032787 GO:0034641 GO:0034754 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043864 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046483 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0072330 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01425.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0165770	sp\|Q9FR37\|AMI1_ARATH	65.083	421	143	3	1	417	1	421	0.0	548

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0165770	G7I823	95.981	423	17	0	1	423	1	423	0.0	840

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0127470	Msa0165770	0.862048	6.677107e-64	-8.615850e-47
Msa0165770	Msa0287010	0.800964	1.125576e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0165770	MtrunA17_Chr1g0191741	95.981	423	17	0	1	423	1	423	0.0	840
Msa0165770	MtrunA17_Chr8g0393041	51.010	396	193	1	28	423	64	458	3.63e-137	405
Msa0165770	MtrunA17_Chr4g0050371	48.341	422	212	2	6	423	48	467	5.34e-130	387
Msa0165770	MtrunA17_Chr2g0279961	37.255	153	95	1	20	172	189	340	1.74e-21	97.1
Msa0165770	MtrunA17_Chr8g0384851	41.611	149	86	1	27	175	203	350	3.31e-19	90.1
Msa0165770	MtrunA17_Chr8g0385461	29.651	172	114	3	26	196	225	390	1.78e-17	84.7
Msa0165770	MtrunA17_Chr3g0097801	47.321	112	59	0	63	174	141	252	2.29e-16	81.3
Msa0165770	MtrunA17_Chr3g0130801	36.913	149	92	1	27	175	267	413	2.30e-16	81.3
Msa0165770	MtrunA17_Chr8g0377651	39.496	119	66	2	63	176	140	257	1.35e-14	75.5
Msa0165770	MtrunA17_Chr8g0377641	38.655	119	67	2	63	176	137	254	5.60e-14	73.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0165770	AT1G08980.1	65.083	421	143	3	1	417	1	421	0.0	548
Msa0165770	AT3G17970.2	51.010	396	189	2	30	423	61	453	2.82e-134	397
Msa0165770	AT3G17970.1	51.010	396	189	2	30	423	61	453	1.35e-133	396
Msa0165770	AT5G09420.1	48.578	422	211	3	6	422	46	466	2.10e-133	396
Msa0165770	AT5G64440.1	35.570	149	95	1	27	175	197	344	2.48e-20	94.0
Msa0165770	AT5G07360.1	33.553	152	94	3	26	176	267	412	1.54e-17	85.5
Msa0165770	AT5G07360.3	33.553	152	94	3	26	176	267	412	1.54e-17	85.5
Msa0165770	AT3G25660.1	46.429	112	60	0	63	174	142	253	3.40e-16	80.9
Msa0165770	AT5G07360.2	32.237	152	89	4	26	176	267	405	7.41e-14	73.9

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Find 135 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGTAATACAGTAGACA+TGG	0.641014	1_4:+46531069	Msa0165770:exon
AAAATCATAGGGACTGATAC+AGG	0.476501	1_4:+46532279	Msa0165770:intron
AAAATCCCACAATATGATGC+AGG	0.541556	1_4:-46532318	None:intergenic
AAACTCCAACAATTGACAGA+AGG	0.641345	1_4:-46534078	None:intergenic
AAACTTACAATGATTGATTA+AGG	0.260467	1_4:-46530993	None:intergenic
AAAGCAATACAGAAGTTGTA+TGG	0.471747	1_4:+46532882	Msa0165770:CDS
AAAGGGTATGTAGCTGGATT+CGG	0.360080	1_4:+46531351	Msa0165770:CDS
AACCCACAAAACAAGGGAAG+AGG	0.585573	1_4:+46531018	Msa0165770:five_prime_UTR
AACGGAGAAAATATACACTA+TGG	0.428548	1_4:+46532030	Msa0165770:CDS
AAGCTTGTGGATTTCTCTCT+AGG	0.437688	1_4:+46532129	Msa0165770:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GCCAATCAAAATTTGATGTT+TGG	+	chr1_4:46530915-46530934	Msa0165770:five_prime_UTR	30.0%
AAAACGTAATACAGTAGACA+TGG	+	chr1_4:46531069-46531088	Msa0165770:exon	30.0%
TCAAACAGATTTGATGTGAA+AGG	+	chr1_4:46531333-46531352	Msa0165770:intron	30.0%
CAAACAGATTTGATGTGAAA+GGG	+	chr1_4:46531334-46531353	Msa0165770:intron	30.0%
TAAAACTGTCATGGATGAAA+TGG	+	chr1_4:46531464-46531483	Msa0165770:CDS	30.0%
CTTTCTTGTCTAAATCAAGT+TGG	+	chr1_4:46531681-46531700	Msa0165770:intron	30.0%
TTCTGAATAATGCCACTAAA+AGG	+	chr1_4:46531775-46531794	Msa0165770:intron	30.0%
ACCCTTGTGATTAGATTTAA+AGG	-	chr1_4:46531841-46531860	None:intergenic	30.0%
TTTAAATCTAATCACAAGGG+TGG	+	chr1_4:46531840-46531859	Msa0165770:intron	30.0%
CAAAAGATACAACTACTCTT+CGG	-	chr1_4:46531935-46531954	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	46530867	46534584	46530867	ID=Msa0165770;Name=Msa0165770
chr1_4	mRNA	46530867	46534584	46530867	ID=Msa0165770-mRNA-1;Parent=Msa0165770;Name=Msa0165770-mRNA-1;_AED=0.30;_eAED=0.30;_QI=221\|1\|1\|1\|1\|1\|9\|262\|423
chr1_4	exon	46530867	46531197	46530867	ID=Msa0165770-mRNA-1:exon:13369;Parent=Msa0165770-mRNA-1
chr1_4	exon	46531341	46531493	46531341	ID=Msa0165770-mRNA-1:exon:13370;Parent=Msa0165770-mRNA-1
chr1_4	exon	46532026	46532150	46532026	ID=Msa0165770-mRNA-1:exon:13371;Parent=Msa0165770-mRNA-1
chr1_4	exon	46532289	46532414	46532289	ID=Msa0165770-mRNA-1:exon:13372;Parent=Msa0165770-mRNA-1
chr1_4	exon	46532724	46532906	46532724	ID=Msa0165770-mRNA-1:exon:13373;Parent=Msa0165770-mRNA-1
chr1_4	exon	46533070	46533217	46533070	ID=Msa0165770-mRNA-1:exon:13374;Parent=Msa0165770-mRNA-1
chr1_4	exon	46533329	46533497	46533329	ID=Msa0165770-mRNA-1:exon:13375;Parent=Msa0165770-mRNA-1
chr1_4	exon	46533980	46534114	46533980	ID=Msa0165770-mRNA-1:exon:13376;Parent=Msa0165770-mRNA-1
chr1_4	exon	46534200	46534584	46534200	ID=Msa0165770-mRNA-1:exon:13377;Parent=Msa0165770-mRNA-1
chr1_4	five_prime_UTR	46530867	46531087	46530867	ID=Msa0165770-mRNA-1:five_prime_utr;Parent=Msa0165770-mRNA-1
chr1_4	CDS	46531088	46531197	46531088	ID=Msa0165770-mRNA-1:cds;Parent=Msa0165770-mRNA-1
chr1_4	CDS	46531341	46531493	46531341	ID=Msa0165770-mRNA-1:cds;Parent=Msa0165770-mRNA-1
chr1_4	CDS	46532026	46532150	46532026	ID=Msa0165770-mRNA-1:cds;Parent=Msa0165770-mRNA-1
chr1_4	CDS	46532289	46532414	46532289	ID=Msa0165770-mRNA-1:cds;Parent=Msa0165770-mRNA-1
chr1_4	CDS	46532724	46532906	46532724	ID=Msa0165770-mRNA-1:cds;Parent=Msa0165770-mRNA-1
chr1_4	CDS	46533070	46533217	46533070	ID=Msa0165770-mRNA-1:cds;Parent=Msa0165770-mRNA-1
chr1_4	CDS	46533329	46533497	46533329	ID=Msa0165770-mRNA-1:cds;Parent=Msa0165770-mRNA-1
chr1_4	CDS	46533980	46534114	46533980	ID=Msa0165770-mRNA-1:cds;Parent=Msa0165770-mRNA-1
chr1_4	CDS	46534200	46534322	46534200	ID=Msa0165770-mRNA-1:cds;Parent=Msa0165770-mRNA-1
chr1_4	three_prime_UTR	46534323	46534584	46534323	ID=Msa0165770-mRNA-1:three_prime_utr;Parent=Msa0165770-mRNA-1

Gene Sequence

>Msa0165770

ATGGAAACAGCCTCAGACTATGGAGCATTCATGGAGAAATTTATTTTACCACCAACTTCTTCTTCCCCTGATCTTCCTCTGAATTCACTCACCTTTGCAGTCAAAGACATATTTGATGTGAAAGGGTATGTAGCTGGATTCGGGAATCCTGATTGGGCAAGGACTCATCAGGCTGCTACTTCTACTGCTCCAACAGTTTTGGCTGTCCTGAGTGCAGGTGCCACTTGTGTTGGTAAAACTGTCATGGATGAAATGGCTTACAGTATAAACGGAGAAAATATACACTATGGCACGCCTAGAAATCCTTGTGCAGAAGACCGAGTACCTGGAGGATCTTCTAGTGGCTCTGCTGTTGCAGTTGGTGCAAAGCTTGTGGATTTCTCTCTAGGGACTGATACAGGAGGAAGTGTAAGAGTTCCTGCATCATATTGTGGGATTTTAGGATTTCGTCCTTCTCATGGCAGAATCTCCAAATCAGGAGTTGTTCCAATGGCACAGAGTTTTGATACTGTTGGATGGTTTGCTAGGGATCCAAAGATTTTAAGTGCAGTTGGACATGTACTTTTGCAATCGCCACCCATCACACCCATTAGACCTACTCAGATTATCATTGCAGAGGATTGTTTTCAGCTTTCAAGTGTTCCTTTCAATGTAGTTTCACAAATTGTCATCAAAGCAATACAGAAGTTGTATGGAGATGGTGTATTAAGGCATGAAATCCTTGGTGAATACGTGAAGGCCAAAGTTCCGGGTTTGAAGCATTTCATGAGTGAAGAGAAAACAGACGAAGTATACAACATTCCATCACTGGCTGCACTTTCAAGTGCCATGCGATTGCTTCAGAGGTATGAATTTAAGAATAACCATGGTGAATGGATCAATGCAGTCAAACCTGATTTAGGTCCGGGAATTTCAGAACGCGTATCAGATGCCCTACGCACAACAGAGGAGAATATTGATATCTGTCATTCTATAATAAGGGAGTTGCGTGATGCTCTTACTTCTCTTCTTGGGGATTTTGGCGTTCTCATGATCCCCACAGTTCCAGGTCCTCCACCTAAACTACAAACAAACTCATCAGACTTGGAAATTTTTCGCGCTAGGGCTTTTAGCCTTCTGTCAATTGTTGGAGTTTCTGGATTTTGTCAGGTAAGCATACCATTAGGGATGCATAATGATCTCCCTGCATCAGTTTCTCTGGTGGCCAGGAATGGTGCAGATGAATTCTTGCTTCACCTTGTGGAGAGTATTTATGATAACATTGAAAAATAG

Protein sequence

>Msa0165770

METASDYGAFMEKFILPPTSSSPDLPLNSLTFAVKDIFDVKGYVAGFGNPDWARTHQAATSTAPTVLAVLSAGATCVGKTVMDEMAYSINGENIHYGTPRNPCAEDRVPGGSSSGSAVAVGAKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGRISKSGVVPMAQSFDTVGWFARDPKILSAVGHVLLQSPPITPIRPTQIIIAEDCFQLSSVPFNVVSQIVIKAIQKLYGDGVLRHEILGEYVKAKVPGLKHFMSEEKTDEVYNIPSLAALSSAMRLLQRYEFKNNHGEWINAVKPDLGPGISERVSDALRTTEENIDICHSIIRELRDALTSLLGDFGVLMIPTVPGPPPKLQTNSSDLEIFRARAFSLLSIVGVSGFCQVSIPLGMHNDLPASVSLVARNGADEFLLHLVESIYDNIEK*