Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0029370 | Msa0169400 | 0.834694 | 2.583924e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATTACTTTGATATGAAAA+AGG | 0.206899 | 1_4:+52641095 | None:intergenic |
| GTTGCTGAGTTCTGCTGTTT+TGG | 0.222251 | 1_4:+52641620 | None:intergenic |
| TCAACCCAGCATCATCTTGC+TGG | 0.398731 | 1_4:+52640994 | None:intergenic |
| CTGAACAGAAAGCCGATAAC+GGG | 0.408377 | 1_4:-52641133 | Msa0169400:intron |
| AGCTACCAGCAAGATGATGC+TGG | 0.437977 | 1_4:-52640999 | Msa0169400:three_prime_UTR |
| TTGATGGCCATGACTTCGGC+AGG | 0.476686 | 1_4:+52641040 | None:intergenic |
| CATGACTTCGGCAGGGTGGA+AGG | 0.497430 | 1_4:+52641048 | None:intergenic |
| AGTGTTACATGCCATCTTGA+TGG | 0.499566 | 1_4:+52641024 | None:intergenic |
| CTTCCACCCTGCCGAAGTCA+TGG | 0.503698 | 1_4:-52641047 | Msa0169400:three_prime_UTR |
| CATCTTGATGGCCATGACTT+CGG | 0.533609 | 1_4:+52641036 | None:intergenic |
| GCTGAACAGAAAGCCGATAA+CGG | 0.538254 | 1_4:-52641134 | Msa0169400:intron |
| CGAAGTCATGGCCATCAAGA+TGG | 0.552488 | 1_4:-52641035 | Msa0169400:three_prime_UTR |
| TTCAGAGGGAGACACGGTAA+AGG | 0.572338 | 1_4:-52641594 | Msa0169400:exon |
| GAACTCAGCAACTCATTCAG+AGG | 0.601465 | 1_4:-52641609 | Msa0169400:CDS |
| GCTACCAGCAAGATGATGCT+GGG | 0.609624 | 1_4:-52640998 | Msa0169400:three_prime_UTR |
| AAATTTATATCAGATGCTTG+AGG | 0.613755 | 1_4:-52641647 | None:intergenic |
| AACTCATTCAGAGGGAGACA+CGG | 0.616699 | 1_4:-52641600 | Msa0169400:CDS |
| AACTCAGCAACTCATTCAGA+GGG | 0.618445 | 1_4:-52641608 | Msa0169400:CDS |
| GCAGGGTGGAAGGAGTAATG+AGG | 0.647114 | 1_4:+52641058 | None:intergenic |
| TGATGGCCATGACTTCGGCA+GGG | 0.674001 | 1_4:+52641041 | None:intergenic |
| TGGCCATGACTTCGGCAGGG+TGG | 0.685541 | 1_4:+52641044 | None:intergenic |
| GGGTGGAAGGAGTAATGAGG+TGG | 0.687051 | 1_4:+52641061 | None:intergenic |
| TGAACAGAAAGCCGATAACG+GGG | 0.706525 | 1_4:-52641132 | Msa0169400:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTACTTTGATATGAAAAA+GGG | + | chr1_4:52641536-52641555 | None:intergenic | 15.0% |
| !! | AAATTACTTTGATATGAAAA+AGG | + | chr1_4:52641537-52641556 | None:intergenic | 15.0% |
| !!! | TTTTAATTCACTTAAACTTC+TGG | - | chr1_4:52641339-52641358 | Msa0169400:intron | 20.0% |
| !!! | TTTTCAAGAGTGCTAGACAA+TGG | + | chr1_4:52641061-52641080 | None:intergenic | 35.0% |
| TTCCTAACTGAACTATCTGA+AGG | + | chr1_4:52641203-52641222 | None:intergenic | 35.0% | |
| TATAAAGCAGCTCACTTTAG+AGG | + | chr1_4:52641267-52641286 | None:intergenic | 35.0% | |
| AAGCATGACTTTCTGCAAAA+GGG | + | chr1_4:52641395-52641414 | None:intergenic | 35.0% | |
| TAAGCATGACTTTCTGCAAA+AGG | + | chr1_4:52641396-52641415 | None:intergenic | 35.0% | |
| !!! | TTGCTGAGTTCTGCTGTTTT+GGG | + | chr1_4:52641011-52641030 | None:intergenic | 40.0% |
| AACTCAGCAACTCATTCAGA+GGG | - | chr1_4:52641021-52641040 | Msa0169400:three_prime_UTR | 40.0% | |
| !!! | TCACTGCTGTTTTGATTCAC+AGG | - | chr1_4:52641109-52641128 | Msa0169400:three_prime_UTR | 40.0% |
| GATTCACAGGTCTTCAACTT+TGG | - | chr1_4:52641122-52641141 | Msa0169400:three_prime_UTR | 40.0% | |
| CACCTTCAGATAGTTCAGTT+AGG | - | chr1_4:52641198-52641217 | Msa0169400:intron | 40.0% | |
| ! | ATGTCTGAGAAGCTTTAGCT+AGG | + | chr1_4:52641426-52641445 | None:intergenic | 40.0% |
| TTCAGCACTCATCAATGTGA+AGG | + | chr1_4:52641481-52641500 | None:intergenic | 40.0% | |
| ! | AGAATTTTGTGCCCCGTTAT+CGG | + | chr1_4:52641511-52641530 | None:intergenic | 40.0% |
| AGTGTTACATGCCATCTTGA+TGG | + | chr1_4:52641608-52641627 | None:intergenic | 40.0% | |
| !! | GTTGCTGAGTTCTGCTGTTT+TGG | + | chr1_4:52641012-52641031 | None:intergenic | 45.0% |
| GAACTCAGCAACTCATTCAG+AGG | - | chr1_4:52641020-52641039 | Msa0169400:three_prime_UTR | 45.0% | |
| AACTCATTCAGAGGGAGACA+CGG | - | chr1_4:52641029-52641048 | Msa0169400:three_prime_UTR | 45.0% | |
| GTTAGGAACCAGAATCCACT+TGG | - | chr1_4:52641215-52641234 | Msa0169400:intron | 45.0% | |
| ! | TATGGACTCCAAGTGGATTC+TGG | + | chr1_4:52641226-52641245 | None:intergenic | 45.0% |
| AGAATCCACTTGGAGTCCAT+AGG | - | chr1_4:52641225-52641244 | Msa0169400:intron | 45.0% | |
| !!! | AGCTTTTTCAGTCCGACCTA+TGG | + | chr1_4:52641244-52641263 | None:intergenic | 45.0% |
| GCTGAACAGAAAGCCGATAA+CGG | - | chr1_4:52641495-52641514 | Msa0169400:intron | 45.0% | |
| CTGAACAGAAAGCCGATAAC+GGG | - | chr1_4:52641496-52641515 | Msa0169400:intron | 45.0% | |
| TGAACAGAAAGCCGATAACG+GGG | - | chr1_4:52641497-52641516 | Msa0169400:intron | 45.0% | |
| ! | CATCTTGATGGCCATGACTT+CGG | + | chr1_4:52641596-52641615 | None:intergenic | 45.0% |
| TTCAGAGGGAGACACGGTAA+AGG | - | chr1_4:52641035-52641054 | Msa0169400:three_prime_UTR | 50.0% | |
| TCCACTTGGAGTCCATAGGT+CGG | - | chr1_4:52641229-52641248 | Msa0169400:intron | 50.0% | |
| CGAAGTCATGGCCATCAAGA+TGG | - | chr1_4:52641594-52641613 | Msa0169400:exon | 50.0% | |
| AGCTACCAGCAAGATGATGC+TGG | - | chr1_4:52641630-52641649 | Msa0169400:CDS | 50.0% | |
| GCTACCAGCAAGATGATGCT+GGG | - | chr1_4:52641631-52641650 | Msa0169400:CDS | 50.0% | |
| TCCGACCTATGGACTCCAAG+TGG | + | chr1_4:52641233-52641252 | None:intergenic | 55.0% | |
| GGGTGGAAGGAGTAATGAGG+TGG | + | chr1_4:52641571-52641590 | None:intergenic | 55.0% | |
| GCAGGGTGGAAGGAGTAATG+AGG | + | chr1_4:52641574-52641593 | None:intergenic | 55.0% | |
| TGATGGCCATGACTTCGGCA+GGG | + | chr1_4:52641591-52641610 | None:intergenic | 55.0% | |
| ! | TTGATGGCCATGACTTCGGC+AGG | + | chr1_4:52641592-52641611 | None:intergenic | 55.0% |
| CATGACTTCGGCAGGGTGGA+AGG | + | chr1_4:52641584-52641603 | None:intergenic | 60.0% | |
| CTTCCACCCTGCCGAAGTCA+TGG | - | chr1_4:52641582-52641601 | Msa0169400:exon | 60.0% | |
| TGGCCATGACTTCGGCAGGG+TGG | + | chr1_4:52641588-52641607 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_4 | gene | 52640995 | 52641656 | 52640995 | ID=Msa0169400;Name=Msa0169400 |
| chr1_4 | mRNA | 52640995 | 52641656 | 52640995 | ID=Msa0169400-mRNA-1;Parent=Msa0169400;Name=Msa0169400-mRNA-1;_AED=0.05;_eAED=1.00;_QI=0|0|0|1|0|0|2|201|19 |
| chr1_4 | exon | 52641549 | 52641656 | 52641549 | ID=Msa0169400-mRNA-1:exon:15676;Parent=Msa0169400-mRNA-1 |
| chr1_4 | exon | 52640995 | 52641147 | 52640995 | ID=Msa0169400-mRNA-1:exon:15675;Parent=Msa0169400-mRNA-1 |
| chr1_4 | CDS | 52641597 | 52641656 | 52641597 | ID=Msa0169400-mRNA-1:cds;Parent=Msa0169400-mRNA-1 |
| chr1_4 | three_prime_UTR | 52641549 | 52641596 | 52641549 | ID=Msa0169400-mRNA-1:three_prime_utr;Parent=Msa0169400-mRNA-1 |
| chr1_4 | three_prime_UTR | 52640995 | 52641147 | 52640995 | ID=Msa0169400-mRNA-1:three_prime_utr;Parent=Msa0169400-mRNA-1 |
| Gene Sequence |
| Protein sequence |
