Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0029370 | Msa0169400 | 0.834694 | 2.583924e-56 | -8.615850e-47 |
| Msa0029370 | Msa0419980 | 0.808001 | 3.838533e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATTACTTTGATATGAAAA+AGG | 0.206899 | 1_1:+54719446 | None:intergenic |
| GTTGCTGAGTTCTGCTGTTT+TGG | 0.222251 | 1_1:+54719971 | None:intergenic |
| TCAACCCAGCATCATCTTGC+TGG | 0.398731 | 1_1:+54719345 | None:intergenic |
| CTGAACAGAAAGCCGATAAC+GGG | 0.408377 | 1_1:-54719484 | Msa0029370:intron |
| AGCTACCAGCAAGATGATGC+TGG | 0.437977 | 1_1:-54719350 | Msa0029370:three_prime_UTR |
| TTGATGGCCATGACTTCGGC+AGG | 0.476686 | 1_1:+54719391 | None:intergenic |
| CATGACTTCGGCAGGGTGGA+AGG | 0.497430 | 1_1:+54719399 | None:intergenic |
| AGTGTTACATGCCATCTTGA+TGG | 0.499566 | 1_1:+54719375 | None:intergenic |
| CTTCCACCCTGCCGAAGTCA+TGG | 0.503698 | 1_1:-54719398 | Msa0029370:three_prime_UTR |
| CATCTTGATGGCCATGACTT+CGG | 0.533609 | 1_1:+54719387 | None:intergenic |
| GCTGAACAGAAAGCCGATAA+CGG | 0.538254 | 1_1:-54719485 | Msa0029370:intron |
| CGAAGTCATGGCCATCAAGA+TGG | 0.552488 | 1_1:-54719386 | Msa0029370:three_prime_UTR |
| TTCAGAGGGAGACACGGTAA+AGG | 0.572338 | 1_1:-54719945 | Msa0029370:exon |
| GAACTCAGCAACTCATTCAG+AGG | 0.601465 | 1_1:-54719960 | Msa0029370:CDS |
| GCTACCAGCAAGATGATGCT+GGG | 0.609624 | 1_1:-54719349 | Msa0029370:three_prime_UTR |
| AAATTTATATCAGATGCTTG+AGG | 0.613755 | 1_1:-54719998 | None:intergenic |
| AACTCATTCAGAGGGAGACA+CGG | 0.616699 | 1_1:-54719951 | Msa0029370:CDS |
| AACTCAGCAACTCATTCAGA+GGG | 0.618445 | 1_1:-54719959 | Msa0029370:CDS |
| GCAGGGTGGAAGGAGTAATG+AGG | 0.647114 | 1_1:+54719409 | None:intergenic |
| TGATGGCCATGACTTCGGCA+GGG | 0.674001 | 1_1:+54719392 | None:intergenic |
| TGGCCATGACTTCGGCAGGG+TGG | 0.685541 | 1_1:+54719395 | None:intergenic |
| GGGTGGAAGGAGTAATGAGG+TGG | 0.687051 | 1_1:+54719412 | None:intergenic |
| TGAACAGAAAGCCGATAACG+GGG | 0.706525 | 1_1:-54719483 | Msa0029370:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTACTTTGATATGAAAAA+GGG | + | chr1_1:54719887-54719906 | None:intergenic | 15.0% |
| !! | AAATTACTTTGATATGAAAA+AGG | + | chr1_1:54719888-54719907 | None:intergenic | 15.0% |
| !!! | TTTTAATTCACTTAAACTTC+TGG | - | chr1_1:54719690-54719709 | Msa0029370:intron | 20.0% |
| !!! | TTTTCAAGAGTGCTAGACAA+TGG | + | chr1_1:54719412-54719431 | None:intergenic | 35.0% |
| TTCCTAACTGAACTATCTGA+AGG | + | chr1_1:54719554-54719573 | None:intergenic | 35.0% | |
| TATAAAGCAGCTCACTTTAG+AGG | + | chr1_1:54719618-54719637 | None:intergenic | 35.0% | |
| AAGCATGACTTTCTGCAAAA+GGG | + | chr1_1:54719746-54719765 | None:intergenic | 35.0% | |
| TAAGCATGACTTTCTGCAAA+AGG | + | chr1_1:54719747-54719766 | None:intergenic | 35.0% | |
| !!! | TTGCTGAGTTCTGCTGTTTT+GGG | + | chr1_1:54719362-54719381 | None:intergenic | 40.0% |
| AACTCAGCAACTCATTCAGA+GGG | - | chr1_1:54719372-54719391 | Msa0029370:three_prime_UTR | 40.0% | |
| !!! | TCACTGCTGTTTTGATTCAC+AGG | - | chr1_1:54719460-54719479 | Msa0029370:three_prime_UTR | 40.0% |
| GATTCACAGGTCTTCAACTT+TGG | - | chr1_1:54719473-54719492 | Msa0029370:three_prime_UTR | 40.0% | |
| CACCTTCAGATAGTTCAGTT+AGG | - | chr1_1:54719549-54719568 | Msa0029370:intron | 40.0% | |
| ! | ATGTCTGAGAAGCTTTAGCT+AGG | + | chr1_1:54719777-54719796 | None:intergenic | 40.0% |
| TTCAGCACTCATCAATGTGA+AGG | + | chr1_1:54719832-54719851 | None:intergenic | 40.0% | |
| ! | AGAATTTTGTGCCCCGTTAT+CGG | + | chr1_1:54719862-54719881 | None:intergenic | 40.0% |
| AGTGTTACATGCCATCTTGA+TGG | + | chr1_1:54719959-54719978 | None:intergenic | 40.0% | |
| !! | GTTGCTGAGTTCTGCTGTTT+TGG | + | chr1_1:54719363-54719382 | None:intergenic | 45.0% |
| GAACTCAGCAACTCATTCAG+AGG | - | chr1_1:54719371-54719390 | Msa0029370:three_prime_UTR | 45.0% | |
| AACTCATTCAGAGGGAGACA+CGG | - | chr1_1:54719380-54719399 | Msa0029370:three_prime_UTR | 45.0% | |
| GTTAGGAACCAGAATCCACT+TGG | - | chr1_1:54719566-54719585 | Msa0029370:intron | 45.0% | |
| ! | TATGGACTCCAAGTGGATTC+TGG | + | chr1_1:54719577-54719596 | None:intergenic | 45.0% |
| AGAATCCACTTGGAGTCCAT+AGG | - | chr1_1:54719576-54719595 | Msa0029370:intron | 45.0% | |
| !!! | AGCTTTTTCAGTCCGACCTA+TGG | + | chr1_1:54719595-54719614 | None:intergenic | 45.0% |
| GCTGAACAGAAAGCCGATAA+CGG | - | chr1_1:54719846-54719865 | Msa0029370:intron | 45.0% | |
| CTGAACAGAAAGCCGATAAC+GGG | - | chr1_1:54719847-54719866 | Msa0029370:intron | 45.0% | |
| TGAACAGAAAGCCGATAACG+GGG | - | chr1_1:54719848-54719867 | Msa0029370:intron | 45.0% | |
| ! | CATCTTGATGGCCATGACTT+CGG | + | chr1_1:54719947-54719966 | None:intergenic | 45.0% |
| TTCAGAGGGAGACACGGTAA+AGG | - | chr1_1:54719386-54719405 | Msa0029370:three_prime_UTR | 50.0% | |
| TCCACTTGGAGTCCATAGGT+CGG | - | chr1_1:54719580-54719599 | Msa0029370:intron | 50.0% | |
| CGAAGTCATGGCCATCAAGA+TGG | - | chr1_1:54719945-54719964 | Msa0029370:exon | 50.0% | |
| AGCTACCAGCAAGATGATGC+TGG | - | chr1_1:54719981-54720000 | Msa0029370:CDS | 50.0% | |
| GCTACCAGCAAGATGATGCT+GGG | - | chr1_1:54719982-54720001 | Msa0029370:CDS | 50.0% | |
| TCCGACCTATGGACTCCAAG+TGG | + | chr1_1:54719584-54719603 | None:intergenic | 55.0% | |
| GGGTGGAAGGAGTAATGAGG+TGG | + | chr1_1:54719922-54719941 | None:intergenic | 55.0% | |
| GCAGGGTGGAAGGAGTAATG+AGG | + | chr1_1:54719925-54719944 | None:intergenic | 55.0% | |
| TGATGGCCATGACTTCGGCA+GGG | + | chr1_1:54719942-54719961 | None:intergenic | 55.0% | |
| ! | TTGATGGCCATGACTTCGGC+AGG | + | chr1_1:54719943-54719962 | None:intergenic | 55.0% |
| CATGACTTCGGCAGGGTGGA+AGG | + | chr1_1:54719935-54719954 | None:intergenic | 60.0% | |
| CTTCCACCCTGCCGAAGTCA+TGG | - | chr1_1:54719933-54719952 | Msa0029370:exon | 60.0% | |
| TGGCCATGACTTCGGCAGGG+TGG | + | chr1_1:54719939-54719958 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 54719346 | 54720007 | 54719346 | ID=Msa0029370;Name=Msa0029370 |
| chr1_1 | mRNA | 54719346 | 54720007 | 54719346 | ID=Msa0029370-mRNA-1;Parent=Msa0029370;Name=Msa0029370-mRNA-1;_AED=0.05;_eAED=1.00;_QI=0|0|0|1|0|0|2|201|19 |
| chr1_1 | exon | 54719900 | 54720007 | 54719900 | ID=Msa0029370-mRNA-1:exon:16345;Parent=Msa0029370-mRNA-1 |
| chr1_1 | exon | 54719346 | 54719498 | 54719346 | ID=Msa0029370-mRNA-1:exon:16344;Parent=Msa0029370-mRNA-1 |
| chr1_1 | CDS | 54719948 | 54720007 | 54719948 | ID=Msa0029370-mRNA-1:cds;Parent=Msa0029370-mRNA-1 |
| chr1_1 | three_prime_UTR | 54719900 | 54719947 | 54719900 | ID=Msa0029370-mRNA-1:three_prime_utr;Parent=Msa0029370-mRNA-1 |
| chr1_1 | three_prime_UTR | 54719346 | 54719498 | 54719346 | ID=Msa0029370-mRNA-1:three_prime_utr;Parent=Msa0029370-mRNA-1 |
| Gene Sequence |
| Protein sequence |
