AlfalfaGEDB | Msa0175130

M. sativa cultivar zhongmu-4 / Msa0175130

KEGG: K11423
GO: GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006325 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008213 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009791 GO:0009987 GO:0010224 GO:0010228 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0019538 GO:0022414 GO:0032259 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046483 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051276 GO:0051716 GO:0061458 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1901564
PFAM: PF17907.4

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0175130	XP_024641768.1	96.451	479	17	0	1	479	1	479	0.0	961

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0175130	sp\|Q84WW6\|ASHH1_ARATH	63.542	480	160	5	6	471	11	489	0.0	612

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0175130	A0A396JTI7	96.451	479	17	0	1	479	1	479	0.0	961

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TFs/TRs:

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Gene ID	Type	Classification
Msa0175130	TR	SET

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0175130	Msa1458860	0.811361	7.277492e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0175130	MtrunA17_Chr1g0201311	96.451	479	17	0	1	479	1	479	0.0	961
Msa0175130	MtrunA17_Chr1g0201621	96.033	479	19	0	1	479	1	479	0.0	958
Msa0175130	MtrunA17_Chr3g0125251	41.026	234	135	3	12	244	1253	1484	7.28e-55	198
Msa0175130	MtrunA17_Chr1g0201321	93.976	83	5	0	271	353	1	83	6.46e-48	159
Msa0175130	MtrunA17_Chr6g0477001	43.519	216	118	4	11	226	42	253	2.08e-47	167
Msa0175130	MtrunA17_Chr2g0305371	34.677	248	145	5	1	248	266	496	5.28e-41	153
Msa0175130	MtrunA17_Chr2g0297191	39.516	124	74	1	96	219	1044	1166	1.40e-22	101
Msa0175130	MtrunA17_Chr5g0403241	37.037	162	87	3	73	219	1341	1502	1.80e-22	101
Msa0175130	MtrunA17_Chr4g0075321	35.366	164	87	3	73	219	898	1059	3.94e-22	100
Msa0175130	MtrunA17_Chr5g0401921	36.095	169	92	3	42	203	672	831	4.36e-21	97.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0175130	AT1G76710.2	63.542	480	160	5	6	471	11	489	0.0	612
Msa0175130	AT1G76710.1	63.542	480	160	5	6	471	11	489	0.0	612
Msa0175130	AT1G76710.4	62.372	489	160	6	6	471	11	498	0.0	604
Msa0175130	AT1G76710.3	60.158	507	160	6	6	471	11	516	0.0	597
Msa0175130	AT1G77300.1	41.631	233	128	4	15	244	958	1185	8.28e-56	201
Msa0175130	AT1G77300.2	41.631	233	128	4	15	244	958	1185	9.09e-56	201
Msa0175130	AT4G30860.1	37.668	223	126	5	7	228	259	469	5.71e-40	150
Msa0175130	AT3G59960.3	46.053	152	81	1	75	226	102	252	3.92e-34	130
Msa0175130	AT3G59960.2	46.053	152	81	1	75	226	102	252	3.92e-34	130
Msa0175130	AT3G59960.4	46.053	152	81	1	75	226	102	252	3.92e-34	130

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Find 132 sgRNAs with CRISPR-Local

Find 475 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGAAGGCCGAGGGTG+GGG	0.484563	1_4:-60964558	Msa0175130:CDS
AAAGTTGGCAATAAGAGGAA+TGG	0.497255	1_4:+60967967	None:intergenic
AACATTTGAGCGCACCACAG+AGG	0.676482	1_4:+60963867	None:intergenic
AACTCTTCTTGCGTTTGCGT+TGG	0.483560	1_4:+60968108	None:intergenic
AAGAAAATGGAATGTCATGG+GGG	0.698806	1_4:-60963990	Msa0175130:CDS
AAGAGTTGGAATATTTGCAC+TGG	0.522066	1_4:-60963963	Msa0175130:CDS
AAGATCCTGTTTGGTATCTT+GGG	0.400986	1_4:+60962754	None:intergenic
AAGCGAGAAAAGGGGTTTGG+GGG	0.578712	1_4:+60968013	None:intergenic
AAGCTTGCAGAAGCGAGAAA+AGG	0.513314	1_4:+60968003	None:intergenic
AAGGCACCCGGCCAAGCAAA+AGG	0.495569	1_4:-60961910	Msa0175130:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TACCATATGAGAACAAAAGT+TGG	+	chr1_4:60961830-60961849	None:intergenic	30.0%
TCACATAAAGTCGAATATGA+AGG	+	chr1_4:60961886-60961905	None:intergenic	30.0%
TGCTATCTTCTACTTATGAT+TGG	-	chr1_4:60962081-60962100	Msa0175130:CDS	30.0%
GTATTCAATTAGGCTTACTT+TGG	-	chr1_4:60962269-60962288	Msa0175130:intron	30.0%
AACATCGATCGATCAATTAA+CGG	-	chr1_4:60962574-60962593	Msa0175130:CDS	30.0%
TATATCCATTGAAGGAAGAT+TGG	+	chr1_4:60962769-60962788	None:intergenic	30.0%
AATGAAAATATCCACTAGCT+GGG	+	chr1_4:60962938-60962957	None:intergenic	30.0%
AAATGAAAATATCCACTAGC+TGG	+	chr1_4:60962939-60962958	None:intergenic	30.0%
ACATTAAAGATGAAAGAGAG+AGG	+	chr1_4:60963204-60963223	None:intergenic	30.0%
AGTAAAAACCATCATTCTCA+TGG	-	chr1_4:60963298-60963317	Msa0175130:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	60961587	60968214	60961587	ID=Msa0175130;Name=Msa0175130
chr1_4	mRNA	60961587	60968214	60961587	ID=Msa0175130-mRNA-1;Parent=Msa0175130;Name=Msa0175130-mRNA-1;_AED=0.15;_eAED=0.15;_QI=257\|1\|1\|1\|1\|1\|10\|259\|479
chr1_4	exon	60961587	60962133	60961587	ID=Msa0175130-mRNA-1:exon:19435;Parent=Msa0175130-mRNA-1
chr1_4	exon	60962574	60962997	60962574	ID=Msa0175130-mRNA-1:exon:19434;Parent=Msa0175130-mRNA-1
chr1_4	exon	60963387	60963421	60963387	ID=Msa0175130-mRNA-1:exon:19433;Parent=Msa0175130-mRNA-1
chr1_4	exon	60963832	60964030	60963832	ID=Msa0175130-mRNA-1:exon:19432;Parent=Msa0175130-mRNA-1
chr1_4	exon	60964169	60964262	60964169	ID=Msa0175130-mRNA-1:exon:19431;Parent=Msa0175130-mRNA-1
chr1_4	exon	60964345	60964429	60964345	ID=Msa0175130-mRNA-1:exon:19430;Parent=Msa0175130-mRNA-1
chr1_4	exon	60964533	60964619	60964533	ID=Msa0175130-mRNA-1:exon:19429;Parent=Msa0175130-mRNA-1
chr1_4	exon	60965606	60965762	60965606	ID=Msa0175130-mRNA-1:exon:19428;Parent=Msa0175130-mRNA-1
chr1_4	exon	60967776	60967843	60967776	ID=Msa0175130-mRNA-1:exon:19427;Parent=Msa0175130-mRNA-1
chr1_4	exon	60967955	60968214	60967955	ID=Msa0175130-mRNA-1:exon:19426;Parent=Msa0175130-mRNA-1
chr1_4	five_prime_UTR	60967958	60968214	60967958	ID=Msa0175130-mRNA-1:five_prime_utr;Parent=Msa0175130-mRNA-1
chr1_4	CDS	60967955	60967957	60967955	ID=Msa0175130-mRNA-1:cds;Parent=Msa0175130-mRNA-1
chr1_4	CDS	60967776	60967843	60967776	ID=Msa0175130-mRNA-1:cds;Parent=Msa0175130-mRNA-1
chr1_4	CDS	60965606	60965762	60965606	ID=Msa0175130-mRNA-1:cds;Parent=Msa0175130-mRNA-1
chr1_4	CDS	60964533	60964619	60964533	ID=Msa0175130-mRNA-1:cds;Parent=Msa0175130-mRNA-1
chr1_4	CDS	60964345	60964429	60964345	ID=Msa0175130-mRNA-1:cds;Parent=Msa0175130-mRNA-1
chr1_4	CDS	60964169	60964262	60964169	ID=Msa0175130-mRNA-1:cds;Parent=Msa0175130-mRNA-1
chr1_4	CDS	60963832	60964030	60963832	ID=Msa0175130-mRNA-1:cds;Parent=Msa0175130-mRNA-1
chr1_4	CDS	60963387	60963421	60963387	ID=Msa0175130-mRNA-1:cds;Parent=Msa0175130-mRNA-1
chr1_4	CDS	60962574	60962997	60962574	ID=Msa0175130-mRNA-1:cds;Parent=Msa0175130-mRNA-1
chr1_4	CDS	60961846	60962133	60961846	ID=Msa0175130-mRNA-1:cds;Parent=Msa0175130-mRNA-1
chr1_4	three_prime_UTR	60961587	60961845	60961587	ID=Msa0175130-mRNA-1:three_prime_utr;Parent=Msa0175130-mRNA-1

Gene Sequence

>Msa0175130

ATGGAACCTCATGCTGCTGAAGATTTGCCTCAATACATTCATATCAATCAAAATGATTTTTTTATGCGAAGGCATAAGAAGCAGAAGGAGGAGGATATTGCTATATGTGAATGCAGGTATGATGAAGATGATCCTGACAGTGCATGTGGAGATGGGTGCCTAAATGTATTAACCAGCACTGAATGCACCCCTGGATTTTGCCCTAGCGGCATCCATTGTAAAAATCAGAAATTTCAGAAGTGTGAATATGCAAAAACAAAGTTATTTAAAACTGAAGGCCGAGGGTGGGGTCTTCTGGCTGATGAGGAAATCAAGGCAGGACAGTTTGTCATTGAATACTGTGGAGAAGTAATATCATGCAAAGAAGCCAAACGCAGATCCCATACTTATGAAATTCAAGGTCTTAAGGATGCATTTATCATTTCTCTTAATGCGTCTGAATCCATCGATGCAACTAGAAAAGGAAGCCTTTCAAGATTTATAAACCATTCCTGTCAACCAAACTGTGAGACAAGAAAATGGAATGTCATGGGGGAAATAAGAGTTGGAATATTTGCACTGGAAGATATTCCTATTGGAACTGAACTGGCATATGACTATAATTTTGAATGGTTTGGAGGTGCAAAGGTTCGCTGCCTCTGTGGTGCGCTCAAATGTTCTGAATTCCTTGGAGCAAAATCTCGTGGTTTTCAAGAGGATACTTATCTATGGGAAGATGATGATGACAGATACTCAATTGAGAAAATTCCTTTATATGACTCTGCGGAGGATGAACTCACGACAAATGTTGGTGTACAGAGTGAACAATCCATGGCTATTATACTGAAAGCTGAAGAACCATCGGAGTCCACTGTTTTAAATGTTCAACCGCTGAATTCGATTGGGATCAATGGCTTAGGTATTCAGAAAATGAAAACTGAAATAGAAAGCGAGGACATGAAGTTGTACTCCCAAGATACCAAACAGGATCTTGCACAAAAAAATGCAATGATATCGCGCATTCAAAGTAACACTGCTGGAGGAAAGTCCATCTCTACCAAAAGATCTAAGGGGGGAAAGCTTAAAAATCACACACAGAAGAAAATTGATGCAAAGTATGCAGCTGGCCTTTTAGCATCAAAGGAAGCACAGGAGGAGATTTTAGATTATGAGAAAAGAAAAGATGACGCTACGGAAGCCCTTGATTCTTTATACAATGAAATACGGCCTGCCATTGAAGAACACGAGAAGGATACCCAAGACAGTGTATCTACGACAGTAGCAGAGAAGTGGATACAGGCTTCCTGCCTAAAACTCAAGGCGGAGTTTGATCTTTACTCGTCAATTATTAAAAATGTTGCTTGTACTCCTCAAAAGGCACCCGGCCAAGCAAAAGGTACTGAAGTCGATAACGAAGACAAAATGAAGCTTTTGACGTTCTCGGCCTCATCTAATCCGTAA

Protein sequence

>Msa0175130

MEPHAAEDLPQYIHINQNDFFMRRHKKQKEEDIAICECRYDEDDPDSACGDGCLNVLTSTECTPGFCPSGIHCKNQKFQKCEYAKTKLFKTEGRGWGLLADEEIKAGQFVIEYCGEVISCKEAKRRSHTYEIQGLKDAFIISLNASESIDATRKGSLSRFINHSCQPNCETRKWNVMGEIRVGIFALEDIPIGTELAYDYNFEWFGGAKVRCLCGALKCSEFLGAKSRGFQEDTYLWEDDDDRYSIEKIPLYDSAEDELTTNVGVQSEQSMAIILKAEEPSESTVLNVQPLNSIGINGLGIQKMKTEIESEDMKLYSQDTKQDLAQKNAMISRIQSNTAGGKSISTKRSKGGKLKNHTQKKIDAKYAAGLLASKEAQEEILDYEKRKDDATEALDSLYNEIRPAIEEHEKDTQDSVSTTVAEKWIQASCLKLKAEFDLYSSIIKNVACTPQKAPGQAKGTEVDNEDKMKLLTFSASSNP*