AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0176990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0176990 MtrunA17_Chr1g0199681 96.805 626 12 4 1 625 76 694 0.0 1246
Msa0176990 MtrunA17_Chr2g0326761 58.087 575 196 14 10 575 2 540 0.0 606
Msa0176990 MtrunA17_Chr4g0028971 57.487 581 210 15 8 577 1 555 0.0 573
Msa0176990 MtrunA17_Chr1g0181211 52.110 616 215 16 1 579 1 573 0.0 561
Msa0176990 MtrunA17_Chr7g0264171 48.792 621 262 16 10 614 22 602 5.84e-177 516
Msa0176990 MtrunA17_Chr7g0264161 54.706 340 111 4 28 363 1 301 1.53e-124 370
Msa0176990 MtrunA17_Chr3g0115271 37.681 621 281 17 16 596 16 570 3.64e-114 353
Msa0176990 MtrunA17_Chr5g0424931 37.671 584 268 22 16 580 16 522 4.56e-100 315
Msa0176990 MtrunA17_Chr5g0417281 37.421 473 216 13 11 465 3 413 6.14e-87 280
Msa0176990 MtrunA17_Chr5g0433301 39.286 392 190 8 9 391 14 366 1.43e-80 271
Msa0176990 MtrunA17_Chr2g0282961 39.474 380 176 8 15 383 51 387 5.59e-80 265
Msa0176990 MtrunA17_Chr4g0070181 40.157 381 180 9 13 385 17 357 5.81e-80 274
Msa0176990 MtrunA17_Chr5g0436761 35.430 477 231 12 8 474 1 410 8.35e-79 257
Msa0176990 MtrunA17_Chr2g0285461 38.107 412 208 8 9 412 13 385 1.46e-78 267
Msa0176990 MtrunA17_Chr3g0109391 38.918 388 189 8 13 391 20 368 1.11e-77 264
Msa0176990 MtrunA17_Chr2g0289221 39.695 393 168 10 16 393 17 355 2.02e-77 250
Msa0176990 MtrunA17_Chr8g0376771 37.713 411 210 8 9 412 13 384 3.94e-77 263
Msa0176990 MtrunA17_Chr4g0046371 39.844 384 179 10 12 383 33 376 4.82e-77 259
Msa0176990 MtrunA17_Chr1g0178011 36.750 400 200 7 14 407 7 359 2.30e-76 251
Msa0176990 MtrunA17_Chr1g0156321 38.750 400 192 11 14 403 38 394 1.32e-74 255
Msa0176990 MtrunA17_Chr8g0385791 37.469 403 203 8 10 404 50 411 2.61e-72 249
Msa0176990 MtrunA17_Chr5g0417211 39.142 373 155 9 16 373 9 324 5.14e-71 235
Msa0176990 MtrunA17_Chr4g0029671 37.440 414 180 10 19 404 54 416 2.11e-70 243
Msa0176990 MtrunA17_Chr8g0389081 38.660 388 188 8 9 386 22 369 4.03e-70 246
Msa0176990 MtrunA17_Chr2g0277461 33.333 519 230 19 19 528 13 424 1.35e-69 233
Msa0176990 MtrunA17_Chr2g0301251 39.578 379 183 11 14 385 18 357 1.77e-69 244
Msa0176990 MtrunA17_Chr2g0326281 37.346 407 182 9 19 393 56 421 4.13e-69 240
Msa0176990 MtrunA17_Chr4g0009751 37.406 401 196 10 15 404 15 371 5.22e-68 233
Msa0176990 MtrunA17_Chr2g0276781 36.869 396 195 7 11 395 15 366 9.11e-68 233
Msa0176990 MtrunA17_Chr7g0239061 35.854 410 205 12 8 405 3 366 1.55e-61 216
Msa0176990 MtrunA17_Chr8g0346711 37.688 398 195 10 16 404 26 379 6.92e-61 214
Msa0176990 MtrunA17_Chr5g0425261 35.677 384 185 15 16 389 9 340 5.31e-56 194
Msa0176990 MtrunA17_Chr5g0424801 35.092 379 185 14 16 384 9 336 2.13e-55 192
Msa0176990 MtrunA17_Chr2g0277531 33.894 357 166 12 19 363 20 318 7.68e-52 181
Msa0176990 MtrunA17_Chr5g0424741 30.078 512 256 19 16 469 9 476 7.09e-48 176
Msa0176990 MtrunA17_Chr5g0425661 42.000 200 95 4 18 206 36 225 6.86e-47 165
Msa0176990 MtrunA17_Chr8g0356211 31.847 314 162 8 126 439 2 263 2.57e-37 141
Msa0176990 MtrunA17_Chr5g0424791 35.526 152 87 4 63 214 11 151 2.24e-20 88.2
Msa0176990 MtrunA17_Chr5g0425651 41.739 115 60 3 277 387 1 112 7.67e-20 85.9
Msa0176990 MtrunA17_Chr1g0178071 35.780 109 67 2 113 218 71 179 1.80e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0176990 AT4G30080.1 53.647 617 230 16 1 604 1 574 0.0 584
Msa0176990 AT2G28350.2 55.594 581 196 16 13 568 4 547 0.0 563
Msa0176990 AT2G28350.1 55.594 581 196 16 13 568 4 547 0.0 563
Msa0176990 AT1G77850.1 38.662 613 301 18 16 600 16 581 6.28e-118 363
Msa0176990 AT1G77850.2 38.662 613 301 18 16 600 16 581 7.11e-118 363
Msa0176990 AT5G37020.2 38.918 388 189 8 13 391 16 364 1.80e-79 267
Msa0176990 AT5G37020.1 38.918 388 189 8 13 391 16 364 5.25e-79 267
Msa0176990 AT1G30330.3 38.517 418 207 10 9 416 14 391 7.85e-78 266
Msa0176990 AT1G30330.2 38.517 418 207 10 9 416 14 391 7.85e-78 266
Msa0176990 AT1G30330.1 38.015 413 207 9 13 416 17 389 1.32e-77 265
Msa0176990 AT1G19850.2 39.119 386 179 9 11 383 35 377 1.06e-74 256
Msa0176990 AT1G19850.1 39.119 386 179 9 11 383 46 388 1.39e-74 256
Msa0176990 AT1G19220.1 38.480 408 193 12 13 409 17 377 1.50e-74 259
Msa0176990 AT2G33860.1 38.636 396 193 9 15 401 52 406 3.31e-74 249
Msa0176990 AT5G60450.2 36.650 412 201 7 8 401 30 399 5.80e-74 252
Msa0176990 AT5G60450.1 37.065 402 193 7 18 401 64 423 8.48e-73 249
Msa0176990 AT5G62000.2 38.155 401 197 8 13 404 55 413 1.11e-71 248
Msa0176990 AT5G62000.5 38.155 401 197 8 13 404 55 413 1.11e-71 248
Msa0176990 AT5G62000.3 38.155 401 197 8 13 404 55 413 1.11e-71 248
Msa0176990 AT5G62000.1 38.155 401 197 8 13 404 55 413 1.11e-71 248
Msa0176990 AT5G62000.4 38.235 408 187 10 13 404 55 413 1.57e-71 247
Msa0176990 AT5G20730.2 33.586 527 271 16 13 508 18 496 4.04e-71 249
Msa0176990 AT5G20730.1 33.523 528 271 17 13 508 18 497 7.96e-70 246
Msa0176990 AT5G20730.3 33.712 528 270 17 13 508 18 497 9.81e-70 246
Msa0176990 AT1G59750.4 38.095 399 197 8 15 404 18 375 2.21e-67 232
Msa0176990 AT1G59750.2 38.095 399 197 8 15 404 18 375 2.57e-67 232
Msa0176990 AT1G59750.1 38.095 399 197 8 15 404 18 375 2.60e-67 232
Msa0176990 AT1G59750.3 38.095 399 197 8 15 404 18 375 2.60e-67 232
Msa0176990 AT2G46530.1 39.211 380 172 13 16 383 18 350 4.87e-66 227
Msa0176990 AT2G46530.3 39.211 380 172 13 16 383 39 371 1.03e-65 227
Msa0176990 AT3G61830.1 36.675 409 197 11 7 404 13 370 2.34e-65 226
Msa0176990 AT4G23980.2 37.065 402 200 11 16 408 9 366 3.66e-64 223
Msa0176990 AT4G23980.1 37.065 402 200 11 16 408 9 366 4.04e-64 223
Msa0176990 AT5G37020.3 37.662 308 151 6 86 391 1 269 6.95e-55 199
Msa0176990 AT1G35540.1 35.294 391 202 9 12 396 20 365 1.87e-54 196
Msa0176990 AT1G35520.1 35.641 390 194 11 12 393 20 360 3.12e-54 195
Msa0176990 AT1G34310.1 36.031 383 188 10 19 393 27 360 3.31e-54 195
Msa0176990 AT3G61830.2 37.536 349 163 10 60 404 22 319 9.48e-54 193
Msa0176990 AT1G35240.1 35.171 381 190 11 12 383 20 352 1.57e-53 193
Msa0176990 AT1G34390.1 34.368 419 209 14 12 422 20 380 3.27e-53 192
Msa0176990 AT2G33860.2 39.298 285 135 5 118 401 2 249 9.54e-53 187
Msa0176990 AT1G34170.1 33.503 394 203 12 19 402 25 369 2.21e-52 188
Msa0176990 AT1G34410.1 34.737 380 193 10 12 383 20 352 7.33e-52 189
Msa0176990 AT2G46530.2 40.370 270 116 8 117 383 36 263 7.41e-48 176
Msa0176990 AT1G34170.2 31.980 394 183 12 19 402 25 343 4.23e-45 167
Msa0176990 AT1G34170.3 31.980 394 183 12 19 402 25 343 3.01e-44 166
Msa0176990 AT1G43950.1 40.654 214 108 7 12 217 20 222 3.42e-38 141
Msa0176990 ATMG00940.1 37.736 159 83 5 205 358 1 148 3.04e-23 97.1
Msa0176990 AT2G36080.3 35.714 112 66 3 112 218 32 142 1.42e-11 65.1
Msa0176990 AT2G36080.1 34.234 111 69 2 112 218 32 142 1.54e-11 65.5
Msa0176990 AT2G36080.4 34.234 111 69 2 112 218 32 142 2.50e-11 63.2
Msa0176990 AT2G36080.2 34.234 111 69 2 112 218 32 142 2.50e-11 63.2
Msa0176990 AT3G25730.1 32.824 131 76 2 118 246 183 303 4.40e-11 65.1
Msa0176990 AT2G46870.1 34.906 106 62 2 110 215 27 125 6.94e-11 64.3

Find 144 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTAGGATTAGTTGGTTTA+TGG 0.157206 1_4:+63704238 Msa0176990:CDS
TTTGAAGAAGATTGTTTCTT+TGG 0.183552 1_4:+63703534 Msa0176990:CDS
ATAAACCTTGGTTTCTTTCT+TGG 0.198649 1_4:-63705081 None:intergenic
TATTGCATAATTCTTCTATT+AGG 0.232046 1_4:+63705862 Msa0176990:three_prime_UTR
ACAAAATATGGTTATGGTTA+TGG 0.233884 1_4:-63705527 None:intergenic
GAGATCTTTGTGTTGGTATA+AGG 0.249926 1_4:+63703871 Msa0176990:CDS
ACGTAGACTTCATCAGTTTC+AGG 0.253211 1_4:-63703471 None:intergenic
GAGAGGTGGAACACGGGTTT+TGG 0.257788 1_4:-63703409 None:intergenic
CTACAACAAGACATGTTTCT+TGG 0.267352 1_4:+63705327 Msa0176990:CDS
TTCTCCTTGGCGCCTTCTTC+AGG 0.267828 1_4:+63704307 Msa0176990:CDS
TTGTTGAGTGTGGGAAATTC+TGG 0.279840 1_4:+63705465 Msa0176990:CDS
GTGTGGTTGGATGAGGAAAA+AGG 0.293287 1_4:-63703173 None:intergenic
CAAAATATGGTTATGGTTAT+GGG 0.304272 1_4:-63705526 None:intergenic
GGAGCCAGGCATGCTCAATT+TGG 0.304628 1_4:+63705261 Msa0176990:CDS
TTGAAACTGAGGATTCTTCT+AGG 0.307810 1_4:+63704222 Msa0176990:CDS
ATTGTTATTGATACTCTTGT+AGG 0.312189 1_4:-63705409 None:intergenic
TCTAGGATTAGTTGGTTTAT+GGG 0.316818 1_4:+63704239 Msa0176990:CDS
CCATAAGGAGGCCTAGTTAT+AGG 0.329666 1_4:-63705384 None:intergenic
AATAGAAGAATTATGCAATA+TGG 0.330857 1_4:-63705859 None:intergenic
CAAATAACAAAATATGGTTA+TGG 0.347669 1_4:-63705533 None:intergenic
TGATTTATGAGGTGAAAATA+TGG 0.352018 1_4:-63705111 None:intergenic
GCTGTAAACTGTGCTGTTAA+TGG 0.368748 1_4:+63704077 Msa0176990:CDS
TGGAGGCTCTCCTTGGTATA+AGG 0.368842 1_4:+63705677 Msa0176990:CDS
CCTATAACTAGGCCTCCTTA+TGG 0.378633 1_4:+63705384 Msa0176990:CDS
TTCTTTCTTGGAGGTGTGAA+TGG 0.380455 1_4:-63705069 None:intergenic
TTTCTAATTCTTCTAGTACT+TGG 0.397904 1_4:+63703934 Msa0176990:CDS
ATGCAAATTCAATGGTGTTC+TGG 0.400255 1_4:+63704179 Msa0176990:CDS
GGAGAATCAGGCCAACGAAT+TGG 0.402668 1_4:-63704290 None:intergenic
CTTTGGAAACAATGGCTTAG+AGG 0.405587 1_4:+63703551 Msa0176990:CDS
AGGATTCTTCTAGGATTAGT+TGG 0.407748 1_4:+63704231 Msa0176990:CDS
TTCGTTGGCCTGATTCTCCT+TGG 0.409284 1_4:+63704294 Msa0176990:CDS
AGAGCAAAGAAAGGTGGAAT+TGG 0.414735 1_4:+63703894 Msa0176990:CDS
AGAAGGCGCCAAGGAGAATC+AGG 0.419546 1_4:-63704302 None:intergenic
AAATGGTGGTAATGATTTGA+TGG 0.424677 1_4:+63704025 Msa0176990:CDS
CAACACAAGCCTGATCTTGT+TGG 0.424795 1_4:+63705705 Msa0176990:CDS
AAATCAGATGGATTATTAGT+TGG 0.429267 1_4:-63705285 None:intergenic
AAACCCTTTGATGAACTAAA+GGG 0.434794 1_4:+63705957 Msa0176990:three_prime_UTR
AAAATTCCTCTTCCACTTGT+GGG 0.435235 1_4:+63706016 Msa0176990:three_prime_UTR
CTTGTCTTGCTTGTTGAGTG+TGG 0.435888 1_4:+63705455 Msa0176990:CDS
CCTTTGTTTGGTGGTGGTGG+TGG 0.436011 1_4:+63703969 Msa0176990:CDS
TTAGCAATAATTGTCTGAAC+AGG 0.436415 1_4:-63703702 None:intergenic
AACCTTGGTTTCTTTCTTGG+AGG 0.437828 1_4:-63705078 None:intergenic
CCATCAGAAGAATTCTCAAC+TGG 0.442393 1_4:-63705657 None:intergenic
AATGTCTTTGCAAAAGAAGC+TGG 0.444449 1_4:-63703585 None:intergenic
GAAACCTTAACACAAAATTC+AGG 0.450898 1_4:-63704140 None:intergenic
TTAAGAATAATTAGCTAGCT+AGG 0.455608 1_4:-63705797 None:intergenic
TAAACCCTTTGATGAACTAA+AGG 0.456993 1_4:+63705956 Msa0176990:three_prime_UTR
ATAGTCTCAGCACAGTATCT+AGG 0.458408 1_4:-63703648 None:intergenic
CTTCTGATGGAGGCTCTCCT+TGG 0.459576 1_4:+63705670 Msa0176990:CDS
CGTAGACTTCATCAGTTTCA+GGG 0.459745 1_4:-63703470 None:intergenic
AGATTAATGTTGGAGATTGA+TGG 0.460974 1_4:-63705159 None:intergenic
TCACCTTTGTTTGGTGGTGG+TGG 0.463200 1_4:+63703966 Msa0176990:CDS
ATGAATGAATTTCTATATCA+AGG 0.463564 1_4:+63706050 Msa0176990:three_prime_UTR
AGTGCTTAGATTCACAACTA+TGG 0.464164 1_4:+63703283 Msa0176990:CDS
AACCGTGTATCACCTTTGTT+TGG 0.470136 1_4:+63703957 Msa0176990:CDS
TTGTTATTGATACTCTTGTA+GGG 0.470816 1_4:-63705408 None:intergenic
AAATGTTACTGCATTGTGTA+TGG 0.470847 1_4:+63705734 Msa0176990:CDS
TTGAAGGGAATTAAATCAGA+TGG 0.471043 1_4:-63705297 None:intergenic
TTGTCTTGCTTGTTGAGTGT+GGG 0.476623 1_4:+63705456 Msa0176990:CDS
GTTTGTAGTTATGGATTCCA+TGG 0.477031 1_4:+63703245 Msa0176990:CDS
TATTATTAGTAGTAGTGTCT+TGG 0.480676 1_4:-63705215 None:intergenic
TGGTCCAAATTGAGCATGCC+TGG 0.482283 1_4:-63705265 None:intergenic
AAGTTTAGACATATCTATAG+AGG 0.484340 1_4:+63703747 Msa0176990:CDS
AATGGTGAGAGATTGAAGTT+TGG 0.485606 1_4:-63705051 None:intergenic
TACCCCTTTAGTTCATCAAA+GGG 0.485698 1_4:-63705960 None:intergenic
AATCACAAGAAGCTTGTTGC+TGG 0.491007 1_4:+63703816 Msa0176990:CDS
AATCTGCAATGCAAATTCAA+TGG 0.492272 1_4:+63704171 Msa0176990:CDS
ATACTCTTGTAGGGTCCATA+AGG 0.493770 1_4:-63705399 None:intergenic
GAAGAATCCTCAGTTTCAAA+AGG 0.493790 1_4:-63704218 None:intergenic
CATAATCCCACAAGTGGAAG+AGG 0.494235 1_4:-63706022 None:intergenic
TTGGTAACTTGTCATTGTCA+AGG 0.495636 1_4:+63705346 Msa0176990:CDS
CATCAAGCATACAGGGAGCC+AGG 0.498276 1_4:+63705247 Msa0176990:CDS
TTGTTGTAGTAGCTTGTTGA+AGG 0.499531 1_4:-63705313 None:intergenic
AATTCAGGAGTACTAGCTCT+TGG 0.500520 1_4:-63704125 None:intergenic
GATTGTTTCTTTGGAAACAA+TGG 0.500964 1_4:+63703543 Msa0176990:CDS
TATGGATCTTGAATAAACCT+TGG 0.506673 1_4:-63705093 None:intergenic
TAACTTGTTTAAGGCAATGA+GGG 0.508770 1_4:+63705993 Msa0176990:three_prime_UTR
CGTGTATCACCTTTGTTTGG+TGG 0.513472 1_4:+63703960 Msa0176990:CDS
GAAAATTCCTCTTCCACTTG+TGG 0.514047 1_4:+63706015 Msa0176990:three_prime_UTR
ATAATATTGTTACCTGAAGA+AGG 0.514309 1_4:-63704319 None:intergenic
CAACTATGGCACGCATGTGC+TGG 0.516615 1_4:+63703297 Msa0176990:CDS
TTTGGAAACAATGGCTTAGA+GGG 0.516902 1_4:+63703552 Msa0176990:CDS
TTACCCCTTTAGTTCATCAA+AGG 0.517930 1_4:-63705961 None:intergenic
GCATTGGAAGTTGATTTATG+AGG 0.518050 1_4:-63705122 None:intergenic
AAGAGTGGTTCTGTTTCTGA+AGG 0.520431 1_4:+63705597 Msa0176990:CDS
GTAATTGAGAAGATTAATGT+TGG 0.520472 1_4:-63705169 None:intergenic
AGTTCAAATATTTCAAAGAG+TGG 0.525439 1_4:+63705582 Msa0176990:CDS
GGTGGTTCTGGTTTCTTGTG+TGG 0.526295 1_4:+63703987 Msa0176990:CDS
AGGATATGCATGGACAGTGT+TGG 0.527527 1_4:+63703724 Msa0176990:CDS
GCAAAGAAAGGTGGAATTGG+TGG 0.533246 1_4:+63703897 Msa0176990:CDS
TAATGATTTGATGGGAAAAG+TGG 0.533977 1_4:+63704034 Msa0176990:CDS
CCAGTTGAGAATTCTTCTGA+TGG 0.534523 1_4:+63705657 Msa0176990:CDS
GTATCACCTTTGTTTGGTGG+TGG 0.534706 1_4:+63703963 Msa0176990:CDS
AGAGAAAGTGAAGGTGTGGT+TGG 0.536093 1_4:-63703186 None:intergenic
GAAAATGGAGATCTTTGTGT+TGG 0.538803 1_4:+63703864 Msa0176990:CDS
TGTTCAGACAATTATTGCTA+AGG 0.546499 1_4:+63703704 Msa0176990:CDS
TTCATAGCAGAGATTCTACA+AGG 0.548674 1_4:-63703435 None:intergenic
TGTTGTTGATCCTTATACCA+AGG 0.550241 1_4:-63705687 None:intergenic
AGTGAAGGTGTGGTTGGATG+AGG 0.550919 1_4:-63703180 None:intergenic
GTTGTCAAAAGATGTCTCCT+TGG 0.557297 1_4:-63703774 None:intergenic
AAGAAAGGTGGAATTGGTGG+TGG 0.559157 1_4:+63703900 Msa0176990:CDS
AAGAGAAAAGAAAAGAAGAG+TGG 0.559701 1_4:-63703142 None:intergenic
GGTGATGATGATGATGGCAT+TGG 0.562514 1_4:-63705138 None:intergenic
GCAATAATTGTCTGAACAGG+TGG 0.564242 1_4:-63703699 None:intergenic
TTTAACATTGTGGAATGTGA+AGG 0.564248 1_4:+63706135 Msa0176990:three_prime_UTR
GAGACTAATTCAACTAACCA+AGG 0.569877 1_4:-63705021 None:intergenic
TTAACTTGTTTAAGGCAATG+AGG 0.574265 1_4:+63705992 Msa0176990:three_prime_UTR
GCACGCATGTGCTGGTGCCA+TGG 0.574468 1_4:+63703305 Msa0176990:CDS
ATAAGGAGAGCAAAGAAAGG+TGG 0.575173 1_4:+63703888 Msa0176990:CDS
TGTTGTAGTAGCTTGTTGAA+GGG 0.575894 1_4:-63705312 None:intergenic
GGTATAAGGAGAGCAAAGAA+AGG 0.576664 1_4:+63703885 Msa0176990:CDS
TAGTTACATAATCCCACAAG+TGG 0.584945 1_4:-63706028 None:intergenic
ATTATTGCTAAGGATATGCA+TGG 0.587806 1_4:+63703714 Msa0176990:CDS
AAAATATGGTTATGGTTATG+GGG 0.588796 1_4:-63705525 None:intergenic
ATGTTCAAGATCCAATTCGT+TGG 0.592882 1_4:+63704279 Msa0176990:CDS
GTTGAGAATTCTTCTGATGG+AGG 0.594043 1_4:+63705660 Msa0176990:CDS
GAGATTCTACAAGGGATGAG+AGG 0.595750 1_4:-63703426 None:intergenic
TCTGCTGAATAATCCAACCT+AGG 0.603231 1_4:-63703675 None:intergenic
GCATGTTCTGCATGACCTTG+TGG 0.603875 1_4:-63703366 None:intergenic
AGACTAATTCAACTAACCAA+GGG 0.605653 1_4:-63705020 None:intergenic
ATCATCAACAACCTATAACT+AGG 0.606131 1_4:+63705373 Msa0176990:CDS
ATGTGAAATGTGTAAACCCT+TGG 0.607377 1_4:+63705004 Msa0176990:CDS
AATCTCTGCTATGAAATACA+TGG 0.608031 1_4:+63703443 Msa0176990:CDS
AATGGTGGTAATGATTTGAT+GGG 0.610306 1_4:+63704026 Msa0176990:CDS
TTAACATTGTGGAATGTGAA+GGG 0.613741 1_4:+63706136 Msa0176990:three_prime_UTR
AAGGGATGAGAGGTGGAACA+CGG 0.623921 1_4:-63703416 None:intergenic
CACCAAACAAAGGTGATACA+CGG 0.628457 1_4:-63703959 None:intergenic
CTCTTGTAGGGTCCATAAGG+AGG 0.630872 1_4:-63705396 None:intergenic
ACACAATCTGATGCAAACAA+TGG 0.631167 1_4:+63703609 Msa0176990:CDS
ATTCTACAAGGGATGAGAGG+TGG 0.633006 1_4:-63703423 None:intergenic
AATGATTTGATGGGAAAAGT+GGG 0.635170 1_4:+63704035 Msa0176990:CDS
ATTGATGGTGATGATGATGA+TGG 0.637177 1_4:-63705144 None:intergenic
CACCTCCAAGAAAGAAACCA+AGG 0.638580 1_4:+63705076 Msa0176990:CDS
AACCCTTTGATGAACTAAAG+GGG 0.646968 1_4:+63705958 Msa0176990:three_prime_UTR
GTTACCTGAAGAAGGCGCCA+AGG 0.651106 1_4:-63704311 None:intergenic
GAGTGGTGGCATTTGAACCA+TGG 0.651177 1_4:-63703322 None:intergenic
AGGGATGAGAGGTGGAACAC+GGG 0.668889 1_4:-63703415 None:intergenic
AAAGTCTTCTACTTTCCACA+AGG 0.669890 1_4:+63703351 Msa0176990:CDS
TCATAGCAGAGATTCTACAA+GGG 0.672161 1_4:-63703434 None:intergenic
TCTGATGCAAACAATGGTGG+TGG 0.672693 1_4:+63703615 Msa0176990:CDS
GTGAAGAGAAAGTGAAGGTG+TGG 0.672989 1_4:-63703190 None:intergenic
CAATCTGATGCAAACAATGG+TGG 0.683286 1_4:+63703612 Msa0176990:CDS
TTGTTGTGAAGAGAAAGTGA+AGG 0.712448 1_4:-63703195 None:intergenic
ATATCTATAGAGGAACACCA+AGG 0.796890 1_4:+63703757 Msa0176990:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTTATTAACTTGTTTA+AGG + chr1_4:63705984-63706003 Msa0176990:three_prime_UTR 10.0%
!!! TTTTTCCAAATAACAAAATA+TGG - chr1_4:63705542-63705561 None:intergenic 15.0%
!!! ATTTTTTTTATCATTGTCTT+TGG + chr1_4:63706075-63706094 Msa0176990:three_prime_UTR 15.0%
!! TAAAAAGATGTTTGTAGTTA+TGG + chr1_4:63703236-63703255 Msa0176990:exon 20.0%
!! TAATTTAATTCTTGCTTAAC+AGG - chr1_4:63704667-63704686 None:intergenic 20.0%
!! CAAATAACAAAATATGGTTA+TGG - chr1_4:63705536-63705555 None:intergenic 20.0%
!!! CATAACCATATTTTGTTATT+TGG + chr1_4:63705534-63705553 Msa0176990:CDS 20.0%
!! AATAGAAGAATTATGCAATA+TGG - chr1_4:63705862-63705881 None:intergenic 20.0%
!! TATTGCATAATTCTTCTATT+AGG + chr1_4:63705862-63705881 Msa0176990:three_prime_UTR 20.0%
!!! TTTTTTTTTGGAACAAAATG+AGG - chr1_4:63705908-63705927 None:intergenic 20.0%
!! ATGAATGAATTTCTATATCA+AGG + chr1_4:63706050-63706069 Msa0176990:three_prime_UTR 20.0%
!!! TTTAGCTTTTTTTAACATTG+TGG + chr1_4:63706125-63706144 Msa0176990:three_prime_UTR 20.0%
!! TTTGAAGAAGATTGTTTCTT+TGG + chr1_4:63703534-63703553 Msa0176990:CDS 25.0%
! AAGTTTAGACATATCTATAG+AGG + chr1_4:63703747-63703766 Msa0176990:CDS 25.0%
! TTTCTAATTCTTCTAGTACT+TGG + chr1_4:63703934-63703953 Msa0176990:CDS 25.0%
! ATAATATTGTTACCTGAAGA+AGG - chr1_4:63704322-63704341 None:intergenic 25.0%
! AACTTAATAACTTTCTCTCA+AGG - chr1_4:63704862-63704881 None:intergenic 25.0%
! TAAGTTTGTACCTACTTAAT+TGG + chr1_4:63704876-63704895 Msa0176990:intron 25.0%
!!! TTCACATTTTGAAGTAAATC+TGG - chr1_4:63704991-63705010 None:intergenic 25.0%
! TGATTTATGAGGTGAAAATA+TGG - chr1_4:63705114-63705133 None:intergenic 25.0%
! GTAATTGAGAAGATTAATGT+TGG - chr1_4:63705172-63705191 None:intergenic 25.0%
! TATTATTAGTAGTAGTGTCT+TGG - chr1_4:63705218-63705237 None:intergenic 25.0%
!!! AAATCAGATGGATTATTAGT+TGG - chr1_4:63705288-63705307 None:intergenic 25.0%
!! TTGTTATTGATACTCTTGTA+GGG - chr1_4:63705411-63705430 None:intergenic 25.0%
!! ATTGTTATTGATACTCTTGT+AGG - chr1_4:63705412-63705431 None:intergenic 25.0%
!! AAATTCTGGTCAAAGTTTTA+AGG + chr1_4:63705479-63705498 Msa0176990:CDS 25.0%
! AAAATATGGTTATGGTTATG+GGG - chr1_4:63705528-63705547 None:intergenic 25.0%
! CAAAATATGGTTATGGTTAT+GGG - chr1_4:63705529-63705548 None:intergenic 25.0%
! ACAAAATATGGTTATGGTTA+TGG - chr1_4:63705530-63705549 None:intergenic 25.0%
! AGTTCAAATATTTCAAAGAG+TGG + chr1_4:63705582-63705601 Msa0176990:CDS 25.0%
!!! AAATGATTTTCAAGACATCA+AGG + chr1_4:63705772-63705791 Msa0176990:three_prime_UTR 25.0%
! TTAAGAATAATTAGCTAGCT+AGG - chr1_4:63705800-63705819 None:intergenic 25.0%
! AAAAACAAATCATACAGCAT+AGG - chr1_4:63705830-63705849 None:intergenic 25.0%
!! TTTTAAGTAGAGATGAAGAC+AGG - chr1_4:63703100-63703119 None:intergenic 30.0%
AAGAGAAAAGAAAAGAAGAG+TGG - chr1_4:63703145-63703164 None:intergenic 30.0%
!!! CATCTTTTTAGAACGAAGTA+GGG - chr1_4:63703226-63703245 None:intergenic 30.0%
!!! ACATCTTTTTAGAACGAAGT+AGG - chr1_4:63703227-63703246 None:intergenic 30.0%
AATCTCTGCTATGAAATACA+TGG + chr1_4:63703443-63703462 Msa0176990:CDS 30.0%
! TAACTCGTTTTCTCTTAAAG+GGG - chr1_4:63703511-63703530 None:intergenic 30.0%
! CTAACTCGTTTTCTCTTAAA+GGG - chr1_4:63703512-63703531 None:intergenic 30.0%
! TCTAACTCGTTTTCTCTTAA+AGG - chr1_4:63703513-63703532 None:intergenic 30.0%
! GATTGTTTCTTTGGAAACAA+TGG + chr1_4:63703543-63703562 Msa0176990:CDS 30.0%
TTAGCAATAATTGTCTGAAC+AGG - chr1_4:63703705-63703724 None:intergenic 30.0%
TGTTCAGACAATTATTGCTA+AGG + chr1_4:63703704-63703723 Msa0176990:CDS 30.0%
ATTATTGCTAAGGATATGCA+TGG + chr1_4:63703714-63703733 Msa0176990:CDS 30.0%
GGAAATGAGAACAGAAAAAA+TGG + chr1_4:63704008-63704027 Msa0176990:CDS 30.0%
AATGAGAACAGAAAAAATGG+TGG + chr1_4:63704011-63704030 Msa0176990:CDS 30.0%
! AAATGGTGGTAATGATTTGA+TGG + chr1_4:63704025-63704044 Msa0176990:CDS 30.0%
! AATGGTGGTAATGATTTGAT+GGG + chr1_4:63704026-63704045 Msa0176990:CDS 30.0%
TAATGATTTGATGGGAAAAG+TGG + chr1_4:63704034-63704053 Msa0176990:CDS 30.0%
AATGATTTGATGGGAAAAGT+GGG + chr1_4:63704035-63704054 Msa0176990:CDS 30.0%
! TGTTAATGGAAGATCTTTTG+AGG + chr1_4:63704091-63704110 Msa0176990:CDS 30.0%
! TACTCCTGAATTTTGTGTTA+AGG + chr1_4:63704136-63704155 Msa0176990:CDS 30.0%
GAAACCTTAACACAAAATTC+AGG - chr1_4:63704143-63704162 None:intergenic 30.0%
AATCTGCAATGCAAATTCAA+TGG + chr1_4:63704171-63704190 Msa0176990:CDS 30.0%
!! TTCTAGGATTAGTTGGTTTA+TGG + chr1_4:63704238-63704257 Msa0176990:CDS 30.0%
!! TCTAGGATTAGTTGGTTTAT+GGG + chr1_4:63704239-63704258 Msa0176990:CDS 30.0%
AATTCAGTGCATCAATAAGA+TGG + chr1_4:63704500-63704519 Msa0176990:intron 30.0%
TTACTTCAAGCCAATTAAGT+AGG - chr1_4:63704889-63704908 None:intergenic 30.0%
! TTTATGATGTCATGTTTTCC+AGG + chr1_4:63704951-63704970 Msa0176990:intron 30.0%
AGACTAATTCAACTAACCAA+GGG - chr1_4:63705023-63705042 None:intergenic 30.0%
ATAAACCTTGGTTTCTTTCT+TGG - chr1_4:63705084-63705103 None:intergenic 30.0%
TATGGATCTTGAATAAACCT+TGG - chr1_4:63705096-63705115 None:intergenic 30.0%
AGATTAATGTTGGAGATTGA+TGG - chr1_4:63705162-63705181 None:intergenic 30.0%
TTGAAGGGAATTAAATCAGA+TGG - chr1_4:63705300-63705319 None:intergenic 30.0%
ATCATCAACAACCTATAACT+AGG + chr1_4:63705373-63705392 Msa0176990:CDS 30.0%
AAATGTTACTGCATTGTGTA+TGG + chr1_4:63705734-63705753 Msa0176990:CDS 30.0%
TAAACCCTTTGATGAACTAA+AGG + chr1_4:63705956-63705975 Msa0176990:three_prime_UTR 30.0%
AAACCCTTTGATGAACTAAA+GGG + chr1_4:63705957-63705976 Msa0176990:three_prime_UTR 30.0%
!! TTAACTTGTTTAAGGCAATG+AGG + chr1_4:63705992-63706011 Msa0176990:three_prime_UTR 30.0%
!! TAACTTGTTTAAGGCAATGA+GGG + chr1_4:63705993-63706012 Msa0176990:three_prime_UTR 30.0%
TTTAACATTGTGGAATGTGA+AGG + chr1_4:63706135-63706154 Msa0176990:three_prime_UTR 30.0%
TTAACATTGTGGAATGTGAA+GGG + chr1_4:63706136-63706155 Msa0176990:three_prime_UTR 30.0%
TTGTTGTGAAGAGAAAGTGA+AGG - chr1_4:63703198-63703217 None:intergenic 35.0%
GTTTGTAGTTATGGATTCCA+TGG + chr1_4:63703245-63703264 Msa0176990:CDS 35.0%
!! CTTTTCAGAGGTTTTTTCCA+TGG - chr1_4:63703265-63703284 None:intergenic 35.0%
! TGAATCTAAGCACTTTTCAG+AGG - chr1_4:63703277-63703296 None:intergenic 35.0%
AGTGCTTAGATTCACAACTA+TGG + chr1_4:63703283-63703302 Msa0176990:CDS 35.0%
! TAGAAGACTTTTGTGTTGAG+TGG - chr1_4:63703342-63703361 None:intergenic 35.0%
AAAGTCTTCTACTTTCCACA+AGG + chr1_4:63703351-63703370 Msa0176990:CDS 35.0%
TCATAGCAGAGATTCTACAA+GGG - chr1_4:63703437-63703456 None:intergenic 35.0%
TTCATAGCAGAGATTCTACA+AGG - chr1_4:63703438-63703457 None:intergenic 35.0%
TTTGGAAACAATGGCTTAGA+GGG + chr1_4:63703552-63703571 Msa0176990:CDS 35.0%
AATGTCTTTGCAAAAGAAGC+TGG - chr1_4:63703588-63703607 None:intergenic 35.0%
ACACAATCTGATGCAAACAA+TGG + chr1_4:63703609-63703628 Msa0176990:CDS 35.0%
!! TGAGACTATTTTTCCTAGGT+TGG + chr1_4:63703662-63703681 Msa0176990:CDS 35.0%
ATATCTATAGAGGAACACCA+AGG + chr1_4:63703757-63703776 Msa0176990:CDS 35.0%
!! GTGTTTTTGAGAGCAGAAAA+TGG + chr1_4:63703849-63703868 Msa0176990:CDS 35.0%
GAAAATGGAGATCTTTGTGT+TGG + chr1_4:63703864-63703883 Msa0176990:CDS 35.0%
! GAGATCTTTGTGTTGGTATA+AGG + chr1_4:63703871-63703890 Msa0176990:CDS 35.0%
ATGCAAATTCAATGGTGTTC+TGG + chr1_4:63704179-63704198 Msa0176990:CDS 35.0%
!!! CAAAATGCCTTTTGAAACTG+AGG + chr1_4:63704211-63704230 Msa0176990:CDS 35.0%
GAAGAATCCTCAGTTTCAAA+AGG - chr1_4:63704221-63704240 None:intergenic 35.0%
TTGAAACTGAGGATTCTTCT+AGG + chr1_4:63704222-63704241 Msa0176990:CDS 35.0%
!! AGGATTCTTCTAGGATTAGT+TGG + chr1_4:63704231-63704250 Msa0176990:CDS 35.0%
ATGTTCAAGATCCAATTCGT+TGG + chr1_4:63704279-63704298 Msa0176990:CDS 35.0%
CTATGGTAGATAGTACTAGT+AGG - chr1_4:63704813-63704832 None:intergenic 35.0%
ATTCTAGCAAGTAGCAACTA+TGG - chr1_4:63704830-63704849 None:intergenic 35.0%
! AGTTGCTACTTGCTAGAATT+TGG + chr1_4:63704831-63704850 Msa0176990:intron 35.0%
ATGTGAAATGTGTAAACCCT+TGG + chr1_4:63705004-63705023 Msa0176990:CDS 35.0%
GAGACTAATTCAACTAACCA+AGG - chr1_4:63705024-63705043 None:intergenic 35.0%
AATGGTGAGAGATTGAAGTT+TGG - chr1_4:63705054-63705073 None:intergenic 35.0%
! GCATTGGAAGTTGATTTATG+AGG - chr1_4:63705125-63705144 None:intergenic 35.0%
!! ATTGATGGTGATGATGATGA+TGG - chr1_4:63705147-63705166 None:intergenic 35.0%
! TTCTTTTGCATCAAGCATAC+AGG + chr1_4:63705239-63705258 Msa0176990:CDS 35.0%
! TCTTTTGCATCAAGCATACA+GGG + chr1_4:63705240-63705259 Msa0176990:CDS 35.0%
!! TGTTGTAGTAGCTTGTTGAA+GGG - chr1_4:63705315-63705334 None:intergenic 35.0%
!! TTGTTGTAGTAGCTTGTTGA+AGG - chr1_4:63705316-63705335 None:intergenic 35.0%
CTACAACAAGACATGTTTCT+TGG + chr1_4:63705327-63705346 Msa0176990:CDS 35.0%
! TTGGTAACTTGTCATTGTCA+AGG + chr1_4:63705346-63705365 Msa0176990:CDS 35.0%
TGTTGTTGATCCTTATACCA+AGG - chr1_4:63705690-63705709 None:intergenic 35.0%
! TTTTCTGTTCCAACAAGATC+AGG - chr1_4:63705717-63705736 None:intergenic 35.0%
AACCCTTTGATGAACTAAAG+GGG + chr1_4:63705958-63705977 Msa0176990:three_prime_UTR 35.0%
! TACCCCTTTAGTTCATCAAA+GGG - chr1_4:63705963-63705982 None:intergenic 35.0%
! TTACCCCTTTAGTTCATCAA+AGG - chr1_4:63705964-63705983 None:intergenic 35.0%
AAAATTCCTCTTCCACTTGT+GGG + chr1_4:63706016-63706035 Msa0176990:three_prime_UTR 35.0%
TAGTTACATAATCCCACAAG+TGG - chr1_4:63706031-63706050 None:intergenic 35.0%
TGTGGTTGGATGAGGAAAAA+GGG - chr1_4:63703175-63703194 None:intergenic 40.0%
! AAGACTTTTGTGTTGAGTGG+TGG - chr1_4:63703339-63703358 None:intergenic 40.0%
CGTAGACTTCATCAGTTTCA+GGG - chr1_4:63703473-63703492 None:intergenic 40.0%
ACGTAGACTTCATCAGTTTC+AGG - chr1_4:63703474-63703493 None:intergenic 40.0%
! CTTTGGAAACAATGGCTTAG+AGG + chr1_4:63703551-63703570 Msa0176990:CDS 40.0%
CAATCTGATGCAAACAATGG+TGG + chr1_4:63703612-63703631 Msa0176990:CDS 40.0%
ATAGTCTCAGCACAGTATCT+AGG - chr1_4:63703651-63703670 None:intergenic 40.0%
!! GTGCTGAGACTATTTTTCCT+AGG + chr1_4:63703658-63703677 Msa0176990:CDS 40.0%
TCTGCTGAATAATCCAACCT+AGG - chr1_4:63703678-63703697 None:intergenic 40.0%
GCAATAATTGTCTGAACAGG+TGG - chr1_4:63703702-63703721 None:intergenic 40.0%
GTTGTCAAAAGATGTCTCCT+TGG - chr1_4:63703777-63703796 None:intergenic 40.0%
! AGGAGACATCTTTTGACAAC+TGG + chr1_4:63703777-63703796 Msa0176990:CDS 40.0%
! GACATCTTTTGACAACTGGT+TGG + chr1_4:63703781-63703800 Msa0176990:CDS 40.0%
!! AATCACAAGAAGCTTGTTGC+TGG + chr1_4:63703816-63703835 Msa0176990:CDS 40.0%
GGTATAAGGAGAGCAAAGAA+AGG + chr1_4:63703885-63703904 Msa0176990:CDS 40.0%
ATAAGGAGAGCAAAGAAAGG+TGG + chr1_4:63703888-63703907 Msa0176990:CDS 40.0%
AGAGCAAAGAAAGGTGGAAT+TGG + chr1_4:63703894-63703913 Msa0176990:CDS 40.0%
AACCGTGTATCACCTTTGTT+TGG + chr1_4:63703957-63703976 Msa0176990:CDS 40.0%
CACCAAACAAAGGTGATACA+CGG - chr1_4:63703962-63703981 None:intergenic 40.0%
! GCTGTAAACTGTGCTGTTAA+TGG + chr1_4:63704077-63704096 Msa0176990:CDS 40.0%
!! AATTCAGGAGTACTAGCTCT+TGG - chr1_4:63704128-63704147 None:intergenic 40.0%
! TCATGTTTTCCAGGTAGTGT+GGG + chr1_4:63704960-63704979 Msa0176990:intron 40.0%
! TTCTTTCTTGGAGGTGTGAA+TGG - chr1_4:63705072-63705091 None:intergenic 40.0%
AACCTTGGTTTCTTTCTTGG+AGG - chr1_4:63705081-63705100 None:intergenic 40.0%
ATACTCTTGTAGGGTCCATA+AGG - chr1_4:63705402-63705421 None:intergenic 40.0%
! TTGTCTTGCTTGTTGAGTGT+GGG + chr1_4:63705456-63705475 Msa0176990:CDS 40.0%
TTGTTGAGTGTGGGAAATTC+TGG + chr1_4:63705465-63705484 Msa0176990:CDS 40.0%
! GGGTGCTTTTGCTTCAATAT+TGG - chr1_4:63705508-63705527 None:intergenic 40.0%
!! AAGAGTGGTTCTGTTTCTGA+AGG + chr1_4:63705597-63705616 Msa0176990:CDS 40.0%
CCATCAGAAGAATTCTCAAC+TGG - chr1_4:63705660-63705679 None:intergenic 40.0%
CCAGTTGAGAATTCTTCTGA+TGG + chr1_4:63705657-63705676 Msa0176990:CDS 40.0%
GTTGAGAATTCTTCTGATGG+AGG + chr1_4:63705660-63705679 Msa0176990:CDS 40.0%
GAAAATTCCTCTTCCACTTG+TGG + chr1_4:63706015-63706034 Msa0176990:three_prime_UTR 40.0%
GTGGTTGGATGAGGAAAAAG+GGG - chr1_4:63703174-63703193 None:intergenic 45.0%
GTGTGGTTGGATGAGGAAAA+AGG - chr1_4:63703176-63703195 None:intergenic 45.0%
! AGAGAAAGTGAAGGTGTGGT+TGG - chr1_4:63703189-63703208 None:intergenic 45.0%
GTGAAGAGAAAGTGAAGGTG+TGG - chr1_4:63703193-63703212 None:intergenic 45.0%
ATTCTACAAGGGATGAGAGG+TGG - chr1_4:63703426-63703445 None:intergenic 45.0%
GAGATTCTACAAGGGATGAG+AGG - chr1_4:63703429-63703448 None:intergenic 45.0%
TCTGATGCAAACAATGGTGG+TGG + chr1_4:63703615-63703634 Msa0176990:CDS 45.0%
AGGATATGCATGGACAGTGT+TGG + chr1_4:63703724-63703743 Msa0176990:CDS 45.0%
GCAAAGAAAGGTGGAATTGG+TGG + chr1_4:63703897-63703916 Msa0176990:CDS 45.0%
AAGAAAGGTGGAATTGGTGG+TGG + chr1_4:63703900-63703919 Msa0176990:CDS 45.0%
! CGTGTATCACCTTTGTTTGG+TGG + chr1_4:63703960-63703979 Msa0176990:CDS 45.0%
! GTATCACCTTTGTTTGGTGG+TGG + chr1_4:63703963-63703982 Msa0176990:CDS 45.0%
! GTCATGTTTTCCAGGTAGTG+TGG + chr1_4:63704959-63704978 Msa0176990:intron 45.0%
CACCTCCAAGAAAGAAACCA+AGG + chr1_4:63705076-63705095 Msa0176990:CDS 45.0%
!! GGTGATGATGATGATGGCAT+TGG - chr1_4:63705141-63705160 None:intergenic 45.0%
CCATAAGGAGGCCTAGTTAT+AGG - chr1_4:63705387-63705406 None:intergenic 45.0%
CCTATAACTAGGCCTCCTTA+TGG + chr1_4:63705384-63705403 Msa0176990:CDS 45.0%
! CTTGTCTTGCTTGTTGAGTG+TGG + chr1_4:63705455-63705474 Msa0176990:CDS 45.0%
CAACACAAGCCTGATCTTGT+TGG + chr1_4:63705705-63705724 Msa0176990:CDS 45.0%
CATAATCCCACAAGTGGAAG+AGG - chr1_4:63706025-63706044 None:intergenic 45.0%
!!! ACATAATATTAATTTTTTTT+TGG - chr1_4:63705920-63705939 None:intergenic 5.0%
! AGTGAAGGTGTGGTTGGATG+AGG - chr1_4:63703183-63703202 None:intergenic 50.0%
GAGTGGTGGCATTTGAACCA+TGG - chr1_4:63703325-63703344 None:intergenic 50.0%
GCATGTTCTGCATGACCTTG+TGG - chr1_4:63703369-63703388 None:intergenic 50.0%
AAGGGATGAGAGGTGGAACA+CGG - chr1_4:63703419-63703438 None:intergenic 50.0%
!! TCACCTTTGTTTGGTGGTGG+TGG + chr1_4:63703966-63703985 Msa0176990:CDS 50.0%
!! GGTGGTTCTGGTTTCTTGTG+TGG + chr1_4:63703987-63704006 Msa0176990:CDS 50.0%
GGAGAATCAGGCCAACGAAT+TGG - chr1_4:63704293-63704312 None:intergenic 50.0%
! TTCGTTGGCCTGATTCTCCT+TGG + chr1_4:63704294-63704313 Msa0176990:CDS 50.0%
TGGTCCAAATTGAGCATGCC+TGG - chr1_4:63705268-63705287 None:intergenic 50.0%
CTCTTGTAGGGTCCATAAGG+AGG - chr1_4:63705399-63705418 None:intergenic 50.0%
TGGAGGCTCTCCTTGGTATA+AGG + chr1_4:63705677-63705696 Msa0176990:CDS 50.0%
CAACTATGGCACGCATGTGC+TGG + chr1_4:63703297-63703316 Msa0176990:CDS 55.0%
! GAGAGGTGGAACACGGGTTT+TGG - chr1_4:63703412-63703431 None:intergenic 55.0%
AGGGATGAGAGGTGGAACAC+GGG - chr1_4:63703418-63703437 None:intergenic 55.0%
CCACCACCACCACCAAACAA+AGG - chr1_4:63703972-63703991 None:intergenic 55.0%
!! CCTTTGTTTGGTGGTGGTGG+TGG + chr1_4:63703969-63703988 Msa0176990:CDS 55.0%
!! TTTGGTGGTGGTGGTGGTTC+TGG + chr1_4:63703975-63703994 Msa0176990:CDS 55.0%
!! AGAAGGCGCCAAGGAGAATC+AGG - chr1_4:63704305-63704324 None:intergenic 55.0%
TTCTCCTTGGCGCCTTCTTC+AGG + chr1_4:63704307-63704326 Msa0176990:CDS 55.0%
!! GTTACCTGAAGAAGGCGCCA+AGG - chr1_4:63704314-63704333 None:intergenic 55.0%
CTGGTTCATCCCACACTACC+TGG - chr1_4:63704972-63704991 None:intergenic 55.0%
CATCAAGCATACAGGGAGCC+AGG + chr1_4:63705247-63705266 Msa0176990:CDS 55.0%
GGAGCCAGGCATGCTCAATT+TGG + chr1_4:63705261-63705280 Msa0176990:CDS 55.0%
CTTCTGATGGAGGCTCTCCT+TGG + chr1_4:63705670-63705689 Msa0176990:CDS 55.0%
! GCACGCATGTGCTGGTGCCA+TGG + chr1_4:63703305-63703324 Msa0176990:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 63703059 63706168 63703059 ID=Msa0176990;Name=Msa0176990
chr1_4 mRNA 63703059 63706168 63703059 ID=Msa0176990-mRNA-1;Parent=Msa0176990;Name=Msa0176990-mRNA-1;_AED=0.00;_eAED=0.00;_QI=184|1|1|1|1|1|2|404|625
chr1_4 exon 63703059 63704328 63703059 ID=Msa0176990-mRNA-1:exon:20661;Parent=Msa0176990-mRNA-1
chr1_4 exon 63704973 63706168 63704973 ID=Msa0176990-mRNA-1:exon:20662;Parent=Msa0176990-mRNA-1
chr1_4 five_prime_UTR 63703059 63703242 63703059 ID=Msa0176990-mRNA-1:five_prime_utr;Parent=Msa0176990-mRNA-1
chr1_4 CDS 63703243 63704328 63703243 ID=Msa0176990-mRNA-1:cds;Parent=Msa0176990-mRNA-1
chr1_4 CDS 63704973 63705764 63704973 ID=Msa0176990-mRNA-1:cds;Parent=Msa0176990-mRNA-1
chr1_4 three_prime_UTR 63705765 63706168 63705765 ID=Msa0176990-mRNA-1:three_prime_utr;Parent=Msa0176990-mRNA-1
Gene Sequence

>Msa0176990

ATGTTTGTAGTTATGGATTCCATGGAAAAAACCTCTGAAAAGTGCTTAGATTCACAACTATGGCACGCATGTGCTGGTGCCATGGTTCAAATGCCACCACTCAACACAAAAGTCTTCTACTTTCCACAAGGTCATGCAGAACATGCTCATAACAAAGTTGATTTTTCCAAAACCCGTGTTCCACCTCTCATCCCTTGTAGAATCTCTGCTATGAAATACATGGCAGACCCTGAAACTGATGAAGTCTACGTCAAAATGAAACTAACCCCTTTAAGAGAAAACGAGTTAGATTTTGAAGAAGATTGTTTCTTTGGAAACAATGGCTTAGAGGGTCAAGAAAAGCCAGCTTCTTTTGCAAAGACATTAACACAATCTGATGCAAACAATGGTGGTGGTTTTTCTGTTCCTAGATACTGTGCTGAGACTATTTTTCCTAGGTTGGATTATTCAGCAGAACCACCTGTTCAGACAATTATTGCTAAGGATATGCATGGACAGTGTTGGAAGTTTAGACATATCTATAGAGGAACACCAAGGAGACATCTTTTGACAACTGGTTGGAGTAATTTTGTGAATCACAAGAAGCTTGTTGCTGGTGATTCAATTGTGTTTTTGAGAGCAGAAAATGGAGATCTTTGTGTTGGTATAAGGAGAGCAAAGAAAGGTGGAATTGGTGGTGGAACTGATCAGTTTTCTAATTCTTCTAGTACTTGGAACCGTGTATCACCTTTGTTTGGTGGTGGTGGTGGTTCTGGTTTCTTGTGTGGAAATGAGAACAGAAAAAATGGTGGTAATGATTTGATGGGAAAAGTGGGAGCTGAATCTGTTGTTGAAGCTGTAAACTGTGCTGTTAATGGAAGATCTTTTGAGGTTGTGTATTATCCAAGAGCTAGTACTCCTGAATTTTGTGTTAAGGTTTCTTCTGTTAAATCTGCAATGCAAATTCAATGGTGTTCTGGTATGAGATTCAAAATGCCTTTTGAAACTGAGGATTCTTCTAGGATTAGTTGGTTTATGGGAACAATTTCTTCTGTTCATGTTCAAGATCCAATTCGTTGGCCTGATTCTCCTTGGCGCCTTCTTCAGGTAGTGTGGGATGAACCAGATTTACTTCAAAATGTGAAATGTGTAAACCCTTGGTTAGTTGAATTAGTCTCAAACATGCCAAACTTCAATCTCTCACCATTCACACCTCCAAGAAAGAAACCAAGGTTTATTCAAGATCCATATTTTCACCTCATAAATCAACTTCCAATGCCATCATCATCATCACCATCAATCTCCAACATTAATCTTCTCAATTACACAAAATCATCTCTCTGTAACATCCAAGACACTACTACTAATAATAGTTCTTTTGCATCAAGCATACAGGGAGCCAGGCATGCTCAATTTGGACCAACTAATAATCCATCTGATTTAATTCCCTTCAACAAGCTACTACAACAAGACATGTTTCTTGGTAACTTGTCATTGTCAAGGTTTAATCATCAACAACCTATAACTAGGCCTCCTTATGGACCCTACAAGAGTATCAATAACAATAACAACACTAAGAACAATGTTGACTTGTCTTGCTTGTTGAGTGTGGGAAATTCTGGTCAAAGTTTTAAGGAATCCAATATTGAAGCAAAAGCACCCCATAACCATAACCATATTTTGTTATTTGGAAAAATCATTCACACTGAACAAAACAGTTCAAATATTTCAAAGAGTGGTTCTGTTTCTGAAGGAACTTCTTTGAAAACATCAAATGCTTCTTCTTCTGATCCAGTTGAGAATTCTTCTGATGGAGGCTCTCCTTGGTATAAGGATCAACAACACAAGCCTGATCTTGTTGGAACAGAAAATGTTACTGCATTGTGTATGGCTTCATAA
Protein sequence

>Msa0176990

MFVVMDSMEKTSEKCLDSQLWHACAGAMVQMPPLNTKVFYFPQGHAEHAHNKVDFSKTRVPPLIPCRISAMKYMADPETDEVYVKMKLTPLRENELDFEEDCFFGNNGLEGQEKPASFAKTLTQSDANNGGGFSVPRYCAETIFPRLDYSAEPPVQTIIAKDMHGQCWKFRHIYRGTPRRHLLTTGWSNFVNHKKLVAGDSIVFLRAENGDLCVGIRRAKKGGIGGGTDQFSNSSSTWNRVSPLFGGGGGSGFLCGNENRKNGGNDLMGKVGAESVVEAVNCAVNGRSFEVVYYPRASTPEFCVKVSSVKSAMQIQWCSGMRFKMPFETEDSSRISWFMGTISSVHVQDPIRWPDSPWRLLQVVWDEPDLLQNVKCVNPWLVELVSNMPNFNLSPFTPPRKKPRFIQDPYFHLINQLPMPSSSSPSISNINLLNYTKSSLCNIQDTTTNNSSFASSIQGARHAQFGPTNNPSDLIPFNKLLQQDMFLGNLSLSRFNHQQPITRPPYGPYKSINNNNNTKNNVDLSCLLSVGNSGQSFKESNIEAKAPHNHNHILLFGKIIHTEQNSSNISKSGSVSEGTSLKTSNASSSDPVENSSDGGSPWYKDQQHKPDLVGTENVTALCMAS*