AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0179360

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0179360 MtrunA17_Chr1g0205211 96.233 292 8 1 1 292 1 289 0.0 565
Msa0179360 MtrunA17_Chr1g0185331 36.735 294 102 10 5 289 17 235 1.51e-43 148
Msa0179360 MtrunA17_Chr7g0260011 37.543 293 107 12 6 289 31 256 5.26e-42 145
Msa0179360 MtrunA17_Chr3g0138651 49.153 118 46 3 186 290 280 396 5.23e-27 108
Msa0179360 MtrunA17_Chr1g0148411 47.368 114 51 2 186 290 204 317 3.38e-25 102
Msa0179360 MtrunA17_Chr2g0331941 29.395 347 162 11 6 281 17 351 1.21e-24 102
Msa0179360 MtrunA17_Chr2g0301141 59.574 47 18 1 242 288 180 225 1.89e-12 65.9
Msa0179360 MtrunA17_Chr4g0070221 35.398 113 57 4 190 288 107 217 3.44e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0179360 AT3G22550.1 43.116 276 109 9 17 288 31 262 4.46e-57 184
Msa0179360 AT3G63210.1 37.801 291 102 14 12 289 37 261 1.13e-37 134
Msa0179360 AT5G11460.1 31.788 302 178 9 3 284 14 307 8.70e-28 110
Msa0179360 AT2G25690.2 29.139 302 172 13 4 288 30 306 2.40e-20 89.7
Msa0179360 AT2G25690.1 29.139 302 172 13 4 288 30 306 2.40e-20 89.7
Msa0179360 AT2G25690.3 27.835 291 169 12 4 277 30 296 2.97e-14 72.0
Msa0179360 AT1G53885.1 56.098 41 18 0 248 288 42 82 5.36e-11 59.7
Msa0179360 AT1G53903.1 56.098 41 18 0 248 288 42 82 5.36e-11 59.7

Find 81 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACAAACATGTTGTGTTCT+AGG 0.159525 1_4:-66873882 None:intergenic
CAAACGTTGTTGGGATAAAT+TGG 0.186420 1_4:+66873911 Msa0179360:CDS
CCATCCTTAGCTTCCAATTT+AGG 0.195239 1_4:-66875282 None:intergenic
ATATGAGTTGTTCTTGGATT+TGG 0.211352 1_4:-66874317 None:intergenic
CTTTCTTTCTCTGCAACTTC+AGG 0.246057 1_4:+66873044 Msa0179360:five_prime_UTR
TCTCATTTGGGTTCTTCTTC+TGG 0.247030 1_4:+66874143 Msa0179360:CDS
TGAAACTAGTGCCCAATTAA+AGG 0.254064 1_4:+66873083 Msa0179360:five_prime_UTR
ATAACACAAGATGCCTAAAT+TGG 0.261760 1_4:+66875269 Msa0179360:exon
TCAAATATATGAGTTGTTCT+TGG 0.277004 1_4:-66874323 None:intergenic
CAACACAGGAAATTCTCATT+TGG 0.290569 1_4:+66874130 Msa0179360:CDS
TCCTCTGGCTACGGCATTCC+TGG 0.294320 1_4:-66875221 None:intergenic
CAATACCCATTCTCTTTAAC+AGG 0.306864 1_4:-66874389 None:intergenic
TTAGGCATCTTGTGTTATAT+AGG 0.310321 1_4:-66875264 None:intergenic
CCATTTGACCTCTGCTTCAA+TGG 0.315431 1_4:+66875335 Msa0179360:three_prime_UTR
GGATTCTTGAAACAAGAAAA+AGG 0.331526 1_4:-66873837 None:intergenic
AGTTTCAGTCTTCCATCATT+AGG 0.333224 1_4:-66873067 None:intergenic
CTGTTTGTTTCAGACCAAAA+AGG 0.336750 1_4:-66873858 None:intergenic
AGAAGAAAGTGGTTTGTGAT+TGG 0.359952 1_4:-66873023 None:intergenic
TTGGAAGCTAAGGATGGTTA+TGG 0.360318 1_4:+66875288 Msa0179360:three_prime_UTR
TTCACAAATTTCACATCAAA+AGG 0.370356 1_4:+66873762 Msa0179360:CDS
AACACAGGAAATTCTCATTT+GGG 0.377926 1_4:+66874131 Msa0179360:CDS
TGGTTTGTGATTGGAGTAAC+TGG 0.380994 1_4:-66873014 None:intergenic
TGACTAAGCTACTACTATAA+TGG 0.381675 1_4:+66873686 Msa0179360:exon
TTCTCTCCTGTTAAAGAGAA+TGG 0.385668 1_4:+66874383 Msa0179360:CDS
GAGAACAAAACTGTTCTGTT+TGG 0.390512 1_4:+66874008 Msa0179360:CDS
GTGAAATTTGTGAACAACTT+TGG 0.393514 1_4:-66873753 None:intergenic
CCTGTGGGAAAATCTTCATT+TGG 0.393590 1_4:+66874182 Msa0179360:CDS
CTAGAAACCTAGAAATGAAA+AGG 0.408277 1_4:-66875372 None:intergenic
ACTACTATAATGGAAGATTC+TGG 0.408639 1_4:+66873696 Msa0179360:exon
TTCTCAACTCAACCTCTCTA+AGG 0.408738 1_4:+66875452 Msa0179360:three_prime_UTR
GTGTCTCTCTGCTAGTGAAA+TGG 0.443405 1_4:+66874256 Msa0179360:CDS
TGAATTAGGGGATTGATGTT+GGG 0.452164 1_4:+66875544 Msa0179360:three_prime_UTR
CAGAGAAAGAAAGAAGAAAG+TGG 0.452250 1_4:-66873034 None:intergenic
AAAGCTCTCAGAGGGATAGC+TGG 0.452672 1_4:-66874426 None:intergenic
CCATTGAAGCAGAGGTCAAA+TGG 0.454772 1_4:-66875335 None:intergenic
TGGTGAACACAAACGTTGTT+GGG 0.456394 1_4:+66873902 Msa0179360:CDS
ATGAATTAGGGGATTGATGT+TGG 0.459928 1_4:+66875543 Msa0179360:three_prime_UTR
GAGTCAAAAGTTGTTGGTCT+TGG 0.463064 1_4:+66873933 Msa0179360:CDS
CACAAACATGTTGTGTTCTA+GGG 0.474240 1_4:-66873881 None:intergenic
TGGAAGCTAAGGATGGTTAT+GGG 0.480328 1_4:+66875289 Msa0179360:three_prime_UTR
GATTGGAGTAACTGGTTGGT+GGG 0.480721 1_4:-66873006 None:intergenic
GATGCCTAAATTGGAAGCTA+AGG 0.481122 1_4:+66875278 Msa0179360:three_prime_UTR
CTGAAATCACCTCTAGATAA+GGG 0.488618 1_4:-66874104 None:intergenic
GAAACTTCAAATTCTACTCA+TGG 0.493090 1_4:+66874221 Msa0179360:CDS
TCTCTCCTGTTAAAGAGAAT+GGG 0.493254 1_4:+66874384 Msa0179360:CDS
CCTAGAACACAACATGTTTG+TGG 0.498494 1_4:+66873882 Msa0179360:CDS
CGTCCTCCAACAACATCCTC+TGG 0.498829 1_4:-66875236 None:intergenic
TCTACTCATGGAGTCTTCAA+AGG 0.500175 1_4:+66874233 Msa0179360:CDS
TGATTGGAGTAACTGGTTGG+TGG 0.502721 1_4:-66873007 None:intergenic
TGCAACTTCAGGCCTAATGA+TGG 0.507985 1_4:+66873055 Msa0179360:five_prime_UTR
CTGCAAGAAGAATCTTGAGC+AGG 0.510629 1_4:+66874466 Msa0179360:CDS
CCGTAGCCAGAGGATGTTGT+TGG 0.510926 1_4:+66875230 Msa0179360:CDS
TATACTCGTGTGATTTCTCA+TGG 0.512508 1_4:+66874293 Msa0179360:CDS
GCTGAAATCACCTCTAGATA+AGG 0.516699 1_4:-66874105 None:intergenic
TTGGTATCAAGTATTGAAGT+AGG 0.516859 1_4:-66873813 None:intergenic
GTAAAGACATCTACATGTAC+AGG 0.532934 1_4:+66874489 Msa0179360:CDS
ATTGCATTGCAGGGGTGAGA+TGG 0.537920 1_4:+66875188 Msa0179360:intron
TTCTAAGTCCATTGAAGCAG+AGG 0.543952 1_4:-66875343 None:intergenic
TATTGAAGTAGGACTCATCA+TGG 0.544546 1_4:-66873802 None:intergenic
TCTCAGAGGGATAGCTGGTA+GGG 0.545126 1_4:-66874421 None:intergenic
CTACTCATGGAGTCTTCAAA+GGG 0.545920 1_4:+66874234 Msa0179360:CDS
TAGAAACCTAGAAATGAAAA+GGG 0.547916 1_4:-66875371 None:intergenic
GTGGTGAACACAAACGTTGT+TGG 0.556870 1_4:+66873901 Msa0179360:CDS
GAGGGATAGCTGGTAGGGTG+AGG 0.560353 1_4:-66874416 None:intergenic
CTCTCAGAGGGATAGCTGGT+AGG 0.568341 1_4:-66874422 None:intergenic
AAATTGGAGTCAAAAGTTGT+TGG 0.568742 1_4:+66873927 Msa0179360:CDS
TTGTGATTGGAGTAACTGGT+TGG 0.569958 1_4:-66873010 None:intergenic
TAGCCAGAGGATGTTGTTGG+AGG 0.574240 1_4:+66875233 Msa0179360:CDS
TGATTATGGCATTATTAATG+TGG 0.576456 1_4:-66875663 None:intergenic
CCTAAATTGGAAGCTAAGGA+TGG 0.577344 1_4:+66875282 Msa0179360:three_prime_UTR
TAGTGAAATGGAGCTTTCTG+AGG 0.587787 1_4:+66874268 Msa0179360:CDS
GCCAGGAATGCCGTAGCCAG+AGG 0.617209 1_4:+66875220 Msa0179360:CDS
TGCAAGAAGAATCTTGAGCA+GGG 0.618568 1_4:+66874467 Msa0179360:CDS
TTTGTGCAGCTACCTTAGAG+AGG 0.620623 1_4:-66875464 None:intergenic
ACACTCAGAAAGCTCTCAGA+GGG 0.629241 1_4:-66874434 None:intergenic
GAATCATCACCCTTATCTAG+AGG 0.629635 1_4:+66874095 Msa0179360:CDS
CCAACAACATCCTCTGGCTA+CGG 0.633843 1_4:-66875230 None:intergenic
GGTGATTTCAGCATCAACAC+AGG 0.637283 1_4:+66874116 Msa0179360:CDS
TACACTCAGAAAGCTCTCAG+AGG 0.674830 1_4:-66874435 None:intergenic
AAAATGCTGAGATATTGTAG+AGG 0.682541 1_4:+66875502 Msa0179360:three_prime_UTR
ACAGTGATATAAGATTTCTG+AGG 0.699760 1_4:-66875408 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAATAAAAGTTAAAAAAAA+TGG - chr1_4:66873209-66873228 None:intergenic 10.0%
!! GGATATATTAATTAAAAAAA+TGG + chr1_4:66874891-66874910 Msa0179360:intron 10.0%
!!! TTTATTTTTTGAAATGAATT+AGG + chr1_4:66875530-66875549 Msa0179360:three_prime_UTR 10.0%
!!! TTATTTTTTGAAATGAATTA+GGG + chr1_4:66875531-66875550 Msa0179360:three_prime_UTR 10.0%
!!! ACTTTTGAAATTTGATTTAT+AGG + chr1_4:66873181-66873200 Msa0179360:intron 15.0%
!!! CTTTTGAAATTTGATTTATA+GGG + chr1_4:66873182-66873201 Msa0179360:intron 15.0%
!! AAATTGAAAGTAAGAAATAA+AGG + chr1_4:66873285-66873304 Msa0179360:intron 15.0%
!!! TTATTTTTCAATTTTCTTAG+TGG + chr1_4:66873496-66873515 Msa0179360:intron 15.0%
!!! TATTTTTTGAAATGAATTAG+GGG + chr1_4:66875532-66875551 Msa0179360:three_prime_UTR 15.0%
!!! TTTTCAATGTACCTTTAATT+GGG - chr1_4:66873097-66873116 None:intergenic 20.0%
!!! ATTTTCAATGTACCTTTAAT+TGG - chr1_4:66873098-66873117 None:intergenic 20.0%
!! TTTAGTAAAGAATATTACAG+AGG + chr1_4:66873519-66873538 Msa0179360:intron 20.0%
!! TGTAGATTATTAGAAAAACT+TGG + chr1_4:66874777-66874796 Msa0179360:intron 20.0%
!!! GAGATTTGATGATAAAATTT+TGG + chr1_4:66874870-66874889 Msa0179360:intron 20.0%
!! ACAACAACAAAAAATAATTG+TGG - chr1_4:66875577-66875596 None:intergenic 20.0%
! TTAGACACAGTAATAATTTG+TGG + chr1_4:66873229-66873248 Msa0179360:intron 25.0%
! TTCTATTTACATTTGTGTCT+TGG - chr1_4:66873267-66873286 None:intergenic 25.0%
! AAATAAAGGTAGCCAAAAAA+AGG + chr1_4:66873299-66873318 Msa0179360:intron 25.0%
!!! TGTAGTTTTGTTCCTTTTTT+TGG - chr1_4:66873314-66873333 None:intergenic 25.0%
! TTTAGATCAGAGAAATAGAT+AGG + chr1_4:66873543-66873562 Msa0179360:intron 25.0%
! TTCACAAATTTCACATCAAA+AGG + chr1_4:66873762-66873781 Msa0179360:CDS 25.0%
!! GTTGAAATTGTTGGTAAAAA+AGG - chr1_4:66874059-66874078 None:intergenic 25.0%
! GAAGAAAAAGTTGAAATTGT+TGG - chr1_4:66874068-66874087 None:intergenic 25.0%
! TCAAATATATGAGTTGTTCT+TGG - chr1_4:66874326-66874345 None:intergenic 25.0%
! ATTAAATATGAGTGTAGTGT+CGG + chr1_4:66874625-66874644 Msa0179360:intron 25.0%
! TATTAGAAAAACTTGGCTTA+TGG + chr1_4:66874784-66874803 Msa0179360:intron 25.0%
! AAAGAGAAAGTAATGTTACA+AGG + chr1_4:66874921-66874940 Msa0179360:intron 25.0%
! TAGAAACCTAGAAATGAAAA+GGG - chr1_4:66875374-66875393 None:intergenic 25.0%
!!! TTTTGTTGTTGTCATTTTGA+TGG + chr1_4:66875585-66875604 Msa0179360:three_prime_UTR 25.0%
! GTAATAATTTGTGGTGAAAG+AGG + chr1_4:66873238-66873257 Msa0179360:intron 30.0%
! TTCTGAGAATAGAATCAAGA+TGG - chr1_4:66873437-66873456 None:intergenic 30.0%
!! TAGGTTTCTGTTTCTATTCA+TGG + chr1_4:66873562-66873581 Msa0179360:intron 30.0%
!!! TCTTGTTTTATGTGTTGTGT+TGG + chr1_4:66873651-66873670 Msa0179360:intron 30.0%
TGACTAAGCTACTACTATAA+TGG + chr1_4:66873686-66873705 Msa0179360:exon 30.0%
! ACTACTATAATGGAAGATTC+TGG + chr1_4:66873696-66873715 Msa0179360:exon 30.0%
GTGAAATTTGTGAACAACTT+TGG - chr1_4:66873756-66873775 None:intergenic 30.0%
!! TTGGTATCAAGTATTGAAGT+AGG - chr1_4:66873816-66873835 None:intergenic 30.0%
GGATTCTTGAAACAAGAAAA+AGG - chr1_4:66873840-66873859 None:intergenic 30.0%
! CTTGTTTCAAGAATCCTTTT+TGG + chr1_4:66873844-66873863 Msa0179360:CDS 30.0%
AAATTGGAGTCAAAAGTTGT+TGG + chr1_4:66873927-66873946 Msa0179360:CDS 30.0%
! GAAATTGTTGGTAAAAAAGG+AGG - chr1_4:66874056-66874075 None:intergenic 30.0%
AACACAGGAAATTCTCATTT+GGG + chr1_4:66874131-66874150 Msa0179360:CDS 30.0%
GAAACTTCAAATTCTACTCA+TGG + chr1_4:66874221-66874240 Msa0179360:CDS 30.0%
ATATGAGTTGTTCTTGGATT+TGG - chr1_4:66874320-66874339 None:intergenic 30.0%
!!! TAGCTTTAGTACTTTTGTAG+AGG + chr1_4:66874848-66874867 Msa0179360:intron 30.0%
TATTCACCATTGTTCTTTCA+AGG - chr1_4:66875134-66875153 None:intergenic 30.0%
TTAGGCATCTTGTGTTATAT+AGG - chr1_4:66875267-66875286 None:intergenic 30.0%
ATAACACAAGATGCCTAAAT+TGG + chr1_4:66875269-66875288 Msa0179360:exon 30.0%
!! TCAAATGGTTTTTGTTTAGG+TGG - chr1_4:66875323-66875342 None:intergenic 30.0%
!! AGGTCAAATGGTTTTTGTTT+AGG - chr1_4:66875326-66875345 None:intergenic 30.0%
! ATAACACCCTTTTCATTTCT+AGG + chr1_4:66875365-66875384 Msa0179360:three_prime_UTR 30.0%
CTAGAAACCTAGAAATGAAA+AGG - chr1_4:66875375-66875394 None:intergenic 30.0%
!! CTTTTCATTTCTAGGTTTCT+AGG + chr1_4:66875373-66875392 Msa0179360:three_prime_UTR 30.0%
ACAGTGATATAAGATTTCTG+AGG - chr1_4:66875411-66875430 None:intergenic 30.0%
AAAATGCTGAGATATTGTAG+AGG + chr1_4:66875502-66875521 Msa0179360:three_prime_UTR 30.0%
! AGAAGAAAGTGGTTTGTGAT+TGG - chr1_4:66873026-66873045 None:intergenic 35.0%
CAGAGAAAGAAAGAAGAAAG+TGG - chr1_4:66873037-66873056 None:intergenic 35.0%
AGTTTCAGTCTTCCATCATT+AGG - chr1_4:66873070-66873089 None:intergenic 35.0%
TGAAACTAGTGCCCAATTAA+AGG + chr1_4:66873083-66873102 Msa0179360:five_prime_UTR 35.0%
! TCTCTTTTATGAGTAGAGTG+GGG - chr1_4:66873138-66873157 None:intergenic 35.0%
! GTCTCTTTTATGAGTAGAGT+GGG - chr1_4:66873139-66873158 None:intergenic 35.0%
! AGTCTCTTTTATGAGTAGAG+TGG - chr1_4:66873140-66873159 None:intergenic 35.0%
TACTCAGTAGTCATCATCAA+AGG - chr1_4:66873382-66873401 None:intergenic 35.0%
TATTGAAGTAGGACTCATCA+TGG - chr1_4:66873805-66873824 None:intergenic 35.0%
CTTGAAACAAGAAAAAGGCT+TGG - chr1_4:66873835-66873854 None:intergenic 35.0%
CTGTTTGTTTCAGACCAAAA+AGG - chr1_4:66873861-66873880 None:intergenic 35.0%
CACAAACATGTTGTGTTCTA+GGG - chr1_4:66873884-66873903 None:intergenic 35.0%
CAAACGTTGTTGGGATAAAT+TGG + chr1_4:66873911-66873930 Msa0179360:CDS 35.0%
!!! AACAGAACAGTTTTGTTCTC+TGG - chr1_4:66874008-66874027 None:intergenic 35.0%
GAGAACAAAACTGTTCTGTT+TGG + chr1_4:66874008-66874027 Msa0179360:CDS 35.0%
CTGAAATCACCTCTAGATAA+GGG - chr1_4:66874107-66874126 None:intergenic 35.0%
CAACACAGGAAATTCTCATT+TGG + chr1_4:66874130-66874149 Msa0179360:CDS 35.0%
TATACTCGTGTGATTTCTCA+TGG + chr1_4:66874293-66874312 Msa0179360:CDS 35.0%
!!! GAGAGTAGTTGTTTTGATGT+TGG + chr1_4:66874359-66874378 Msa0179360:CDS 35.0%
TTCTCTCCTGTTAAAGAGAA+TGG + chr1_4:66874383-66874402 Msa0179360:CDS 35.0%
TCTCTCCTGTTAAAGAGAAT+GGG + chr1_4:66874384-66874403 Msa0179360:CDS 35.0%
CAATACCCATTCTCTTTAAC+AGG - chr1_4:66874392-66874411 None:intergenic 35.0%
GTAAAGACATCTACATGTAC+AGG + chr1_4:66874489-66874508 Msa0179360:CDS 35.0%
AAAAACTTGGCTTATGGTGT+TGG + chr1_4:66874790-66874809 Msa0179360:intron 35.0%
! ATTTTGCCCTTGATCATCAA+TGG + chr1_4:66874823-66874842 Msa0179360:intron 35.0%
! TTTTGCCCTTGATCATCAAT+GGG + chr1_4:66874824-66874843 Msa0179360:intron 35.0%
ACGATTCCTTGAAAGAACAA+TGG + chr1_4:66875125-66875144 Msa0179360:intron 35.0%
TGCAATGCAATAATGCAACT+AGG - chr1_4:66875179-66875198 None:intergenic 35.0%
TAGTTGCATTATTGCATTGC+AGG + chr1_4:66875178-66875197 Msa0179360:intron 35.0%
AGTTGCATTATTGCATTGCA+GGG + chr1_4:66875179-66875198 Msa0179360:intron 35.0%
! TAGCTGCACAAAATTTTGAG+AGG + chr1_4:66875475-66875494 Msa0179360:three_prime_UTR 35.0%
ATGAATTAGGGGATTGATGT+TGG + chr1_4:66875543-66875562 Msa0179360:three_prime_UTR 35.0%
TGAATTAGGGGATTGATGTT+GGG + chr1_4:66875544-66875563 Msa0179360:three_prime_UTR 35.0%
TTGTGATTGGAGTAACTGGT+TGG - chr1_4:66873013-66873032 None:intergenic 40.0%
! TGGTTTGTGATTGGAGTAAC+TGG - chr1_4:66873017-66873036 None:intergenic 40.0%
CTTTCTTTCTCTGCAACTTC+AGG + chr1_4:66873044-66873063 Msa0179360:five_prime_UTR 40.0%
! GATGGTGATGAAAAACGACA+TGG - chr1_4:66873419-66873438 None:intergenic 40.0%
CAGAAACAGTGCAAAAAAGC+CGG + chr1_4:66873452-66873471 Msa0179360:intron 40.0%
AGAAACAGTGCAAAAAAGCC+GGG + chr1_4:66873453-66873472 Msa0179360:intron 40.0%
!!! TAGTAGTGTTACTTTTCCCC+CGG - chr1_4:66873474-66873493 None:intergenic 40.0%
CCACAAACATGTTGTGTTCT+AGG - chr1_4:66873885-66873904 None:intergenic 40.0%
CCTAGAACACAACATGTTTG+TGG + chr1_4:66873882-66873901 Msa0179360:CDS 40.0%
TGGTGAACACAAACGTTGTT+GGG + chr1_4:66873902-66873921 Msa0179360:CDS 40.0%
! GAGTCAAAAGTTGTTGGTCT+TGG + chr1_4:66873933-66873952 Msa0179360:CDS 40.0%
GAATCATCACCCTTATCTAG+AGG + chr1_4:66874095-66874114 Msa0179360:CDS 40.0%
GCTGAAATCACCTCTAGATA+AGG - chr1_4:66874108-66874127 None:intergenic 40.0%
! TCTCATTTGGGTTCTTCTTC+TGG + chr1_4:66874143-66874162 Msa0179360:CDS 40.0%
! CCAAATGAAGATTTTCCCAC+AGG - chr1_4:66874185-66874204 None:intergenic 40.0%
CCTGTGGGAAAATCTTCATT+TGG + chr1_4:66874182-66874201 Msa0179360:CDS 40.0%
TCTACTCATGGAGTCTTCAA+AGG + chr1_4:66874233-66874252 Msa0179360:CDS 40.0%
CTACTCATGGAGTCTTCAAA+GGG + chr1_4:66874234-66874253 Msa0179360:CDS 40.0%
TAGTGAAATGGAGCTTTCTG+AGG + chr1_4:66874268-66874287 Msa0179360:CDS 40.0%
TGCAAGAAGAATCTTGAGCA+GGG + chr1_4:66874467-66874486 Msa0179360:CDS 40.0%
TTTGCCCTTGATCATCAATG+GGG + chr1_4:66874825-66874844 Msa0179360:intron 40.0%
TGTCAGACACTAACACAACA+TGG + chr1_4:66874982-66875001 Msa0179360:intron 40.0%
TTCCTTTGATGTCACAGACA+CGG - chr1_4:66875066-66875085 None:intergenic 40.0%
GTTCAAGATCTCATGTCTCA+TGG - chr1_4:66875157-66875176 None:intergenic 40.0%
GTTGCATTATTGCATTGCAG+GGG + chr1_4:66875180-66875199 Msa0179360:intron 40.0%
GATGCCTAAATTGGAAGCTA+AGG + chr1_4:66875278-66875297 Msa0179360:three_prime_UTR 40.0%
CCATCCTTAGCTTCCAATTT+AGG - chr1_4:66875285-66875304 None:intergenic 40.0%
CCTAAATTGGAAGCTAAGGA+TGG + chr1_4:66875282-66875301 Msa0179360:three_prime_UTR 40.0%
TTGGAAGCTAAGGATGGTTA+TGG + chr1_4:66875288-66875307 Msa0179360:three_prime_UTR 40.0%
TGGAAGCTAAGGATGGTTAT+GGG + chr1_4:66875289-66875308 Msa0179360:three_prime_UTR 40.0%
TTCTAAGTCCATTGAAGCAG+AGG - chr1_4:66875346-66875365 None:intergenic 40.0%
TTCTCAACTCAACCTCTCTA+AGG + chr1_4:66875452-66875471 Msa0179360:three_prime_UTR 40.0%
GATTGGAGTAACTGGTTGGT+GGG - chr1_4:66873009-66873028 None:intergenic 45.0%
TGATTGGAGTAACTGGTTGG+TGG - chr1_4:66873010-66873029 None:intergenic 45.0%
TGCAACTTCAGGCCTAATGA+TGG + chr1_4:66873055-66873074 Msa0179360:five_prime_UTR 45.0%
TCTCTGTTACATGCACGTCT+TGG + chr1_4:66873355-66873374 Msa0179360:intron 45.0%
GAAACAGTGCAAAAAAGCCG+GGG + chr1_4:66873454-66873473 Msa0179360:intron 45.0%
AAACAGTGCAAAAAAGCCGG+GGG + chr1_4:66873455-66873474 Msa0179360:intron 45.0%
GTGGTGAACACAAACGTTGT+TGG + chr1_4:66873901-66873920 Msa0179360:CDS 45.0%
GGTGATTTCAGCATCAACAC+AGG + chr1_4:66874116-66874135 Msa0179360:CDS 45.0%
! TAGCTTTTCACTCTCTCCTG+TGG + chr1_4:66874166-66874185 Msa0179360:CDS 45.0%
! AGCTTTTCACTCTCTCCTGT+GGG + chr1_4:66874167-66874186 Msa0179360:CDS 45.0%
GTGTCTCTCTGCTAGTGAAA+TGG + chr1_4:66874256-66874275 Msa0179360:CDS 45.0%
ACACTCAGAAAGCTCTCAGA+GGG - chr1_4:66874437-66874456 None:intergenic 45.0%
TACACTCAGAAAGCTCTCAG+AGG - chr1_4:66874438-66874457 None:intergenic 45.0%
CTGCAAGAAGAATCTTGAGC+AGG + chr1_4:66874466-66874485 Msa0179360:CDS 45.0%
! CTGCTTATTCTTTTACCCCC+AGG + chr1_4:66874515-66874534 Msa0179360:intron 45.0%
ATCTAGTGTCTCACACGTGT+TGG - chr1_4:66874654-66874673 None:intergenic 45.0%
! GCTACCCCATTGATGATCAA+GGG - chr1_4:66874832-66874851 None:intergenic 45.0%
! AGCTACCCCATTGATGATCA+AGG - chr1_4:66874833-66874852 None:intergenic 45.0%
! ATGTTACAAGGCGTCTCTTG+TGG + chr1_4:66874933-66874952 Msa0179360:intron 45.0%
CCATTGAAGCAGAGGTCAAA+TGG - chr1_4:66875338-66875357 None:intergenic 45.0%
CCATTTGACCTCTGCTTCAA+TGG + chr1_4:66875335-66875354 Msa0179360:three_prime_UTR 45.0%
TTTGTGCAGCTACCTTAGAG+AGG - chr1_4:66875467-66875486 None:intergenic 45.0%
TCTCAGAGGGATAGCTGGTA+GGG - chr1_4:66874424-66874443 None:intergenic 50.0%
AAAGCTCTCAGAGGGATAGC+TGG - chr1_4:66874429-66874448 None:intergenic 50.0%
!! GTGCTTGTTATCATGCCTGG+GGG - chr1_4:66874533-66874552 None:intergenic 50.0%
!! GGTGCTTGTTATCATGCCTG+GGG - chr1_4:66874534-66874553 None:intergenic 50.0%
!! CGGTGCTTGTTATCATGCCT+GGG - chr1_4:66874535-66874554 None:intergenic 50.0%
!! TCGGTGCTTGTTATCATGCC+TGG - chr1_4:66874536-66874555 None:intergenic 50.0%
! AGGCATGATAACAAGCACCG+AGG + chr1_4:66874535-66874554 Msa0179360:intron 50.0%
!! AACAAGCACCGAGGCAAAAC+TGG + chr1_4:66874544-66874563 Msa0179360:intron 50.0%
TGCCGTGTCTGTGACATCAA+AGG + chr1_4:66875061-66875080 Msa0179360:intron 50.0%
ATTGCATTGCAGGGGTGAGA+TGG + chr1_4:66875188-66875207 Msa0179360:intron 50.0%
! TGAGATGGCATTTTGCAGCC+AGG + chr1_4:66875203-66875222 Msa0179360:CDS 50.0%
CCAACAACATCCTCTGGCTA+CGG - chr1_4:66875233-66875252 None:intergenic 50.0%
TAGCCAGAGGATGTTGTTGG+AGG + chr1_4:66875233-66875252 Msa0179360:CDS 50.0%
CTCTCAGAGGGATAGCTGGT+AGG - chr1_4:66874425-66874444 None:intergenic 55.0%
CACCGAGGCAAAACTGGACA+CGG + chr1_4:66874550-66874569 Msa0179360:intron 55.0%
! TGCCGTGTCCAGTTTTGCCT+CGG - chr1_4:66874555-66874574 None:intergenic 55.0%
!! AACGTTTTTCGACCCACGCG+TGG - chr1_4:66874960-66874979 None:intergenic 55.0%
CCGTAGCCAGAGGATGTTGT+TGG + chr1_4:66875230-66875249 Msa0179360:CDS 55.0%
CGTCCTCCAACAACATCCTC+TGG - chr1_4:66875239-66875258 None:intergenic 55.0%
GAGGGATAGCTGGTAGGGTG+AGG - chr1_4:66874419-66874438 None:intergenic 60.0%
GTCTCTTGTGGTCCACGCGT+GGG + chr1_4:66874945-66874964 Msa0179360:intron 60.0%
TCCTCTGGCTACGGCATTCC+TGG - chr1_4:66875224-66875243 None:intergenic 60.0%
CGTCTCTTGTGGTCCACGCG+TGG + chr1_4:66874944-66874963 Msa0179360:intron 65.0%
GCCAGGAATGCCGTAGCCAG+AGG + chr1_4:66875220-66875239 Msa0179360:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 66872983 66875681 66872983 ID=Msa0179360;Name=Msa0179360
chr1_4 mRNA 66872983 66875681 66872983 ID=Msa0179360-mRNA-1;Parent=Msa0179360;Name=Msa0179360-mRNA-1;_AED=0.19;_eAED=0.19;_QI=145|1|1|1|0.5|0.33|3|409|292
chr1_4 exon 66872983 66873104 66872983 ID=Msa0179360-mRNA-1:exon:21922;Parent=Msa0179360-mRNA-1
chr1_4 exon 66873682 66874510 66873682 ID=Msa0179360-mRNA-1:exon:21923;Parent=Msa0179360-mRNA-1
chr1_4 exon 66875200 66875681 66875200 ID=Msa0179360-mRNA-1:exon:21924;Parent=Msa0179360-mRNA-1
chr1_4 five_prime_UTR 66872983 66873104 66872983 ID=Msa0179360-mRNA-1:five_prime_utr;Parent=Msa0179360-mRNA-1
chr1_4 five_prime_UTR 66873682 66873704 66873682 ID=Msa0179360-mRNA-1:five_prime_utr;Parent=Msa0179360-mRNA-1
chr1_4 CDS 66873705 66874510 66873705 ID=Msa0179360-mRNA-1:cds;Parent=Msa0179360-mRNA-1
chr1_4 CDS 66875200 66875272 66875200 ID=Msa0179360-mRNA-1:cds;Parent=Msa0179360-mRNA-1
chr1_4 three_prime_UTR 66875273 66875681 66875273 ID=Msa0179360-mRNA-1:three_prime_utr;Parent=Msa0179360-mRNA-1
Gene Sequence

>Msa0179360

ATGGAAGATTCTGGTTCAAAGAAAATCACTTCATCTTTCTTCAATTCTCCAAAGTTGTTCACAAATTTCACATCAAAAGGTTTTCATGAAACTGAAACCATGATGAGTCCTACTTCAATACTTGATACCAAGCCTTTTTCTTGTTTCAAGAATCCTTTTTGGTCTGAAACAAACAGCCCTAGAACACAACATGTTTGTGGTGAACACAAACGTTGTTGGGATAAATTGGAGTCAAAAGTTGTTGGTCTTGGTCTTGTTGATGTTCTTGTAGATGAAAAAAACAGTGAAGTGAATTCAAAACCAGAGAACAAAACTGTTCTGTTTGGATCTCAGTTGAAGATTCAGATTCCTCCTTTTTTACCAACAATTTCAACTTTTTCTTCTTCATCTGAATCATCACCCTTATCTAGAGGTGATTTCAGCATCAACACAGGAAATTCTCATTTGGGTTCTTCTTCTGGTAGCTTTTCACTCTCTCCTGTGGGAAAATCTTCATTTGGATGTGCTAATGAAATTGAAACTTCAAATTCTACTCATGGAGTCTTCAAAGGGTGTCTCTCTGCTAGTGAAATGGAGCTTTCTGAGGATTATACTCGTGTGATTTCTCATGGTCCAAATCCAAGAACAACTCATATATTTGATAATTGCATAATTGAGAGTAGTTGTTTTGATGTTGGATTCTCTCCTGTTAAAGAGAATGGGTATTGTTTTCCTCACCCTACCAGCTATCCCTCTGAGAGCTTTCTGAGTGTATGTTTTCACTGCAAGAAGAATCTTGAGCAGGGTAAAGACATCTACATGTACAGGGGTGAGATGGCATTTTGCAGCCAGGAATGCCGTAGCCAGAGGATGTTGTTGGAGGACGTGATGCCTATATAA
Protein sequence

>Msa0179360

MEDSGSKKITSSFFNSPKLFTNFTSKGFHETETMMSPTSILDTKPFSCFKNPFWSETNSPRTQHVCGEHKRCWDKLESKVVGLGLVDVLVDEKNSEVNSKPENKTVLFGSQLKIQIPPFLPTISTFSSSSESSPLSRGDFSINTGNSHLGSSSGSFSLSPVGKSSFGCANEIETSNSTHGVFKGCLSASEMELSEDYTRVISHGPNPRTTHIFDNCIIESSCFDVGFSPVKENGYCFPHPTSYPSESFLSVCFHCKKNLEQGKDIYMYRGEMAFCSQECRSQRMLLEDVMPI*