AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0179780

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0179780 MtrunA17_Chr1g0205701 96.757 185 6 0 1 185 1 185 5.38e-118 332
Msa0179780 MtrunA17_Chr5g0394321 71.515 165 40 3 1 164 1 159 6.67e-86 250
Msa0179780 MtrunA17_Chr4g0064041 39.264 163 89 3 1 157 1 159 2.76e-27 105
Msa0179780 MtrunA17_Chr4g0064041 46.000 100 52 1 25 122 191 290 1.22e-26 103
Msa0179780 MtrunA17_Chr8g0335531 33.125 160 88 6 12 161 11 161 1.08e-26 100
Msa0179780 MtrunA17_Chr2g0323021 37.013 154 81 6 8 148 6 156 3.69e-24 94.7
Msa0179780 MtrunA17_Chr7g0253761 36.571 175 104 4 1 173 1 170 2.79e-23 91.3
Msa0179780 MtrunA17_Chr1g0177341 38.017 121 71 3 6 124 5 123 3.18e-23 90.5
Msa0179780 MtrunA17_Chr1g0177331 38.017 121 71 3 6 124 5 123 3.18e-23 90.5
Msa0179780 MtrunA17_Chr7g0253681 38.095 126 73 4 1 123 1 124 1.18e-22 89.0
Msa0179780 MtrunA17_Chr2g0331181 34.266 143 84 4 19 158 21 156 2.15e-22 89.0
Msa0179780 MtrunA17_Chr7g0253701 36.800 125 75 3 1 123 1 123 2.40e-22 88.2
Msa0179780 MtrunA17_Chr7g0253671 36.800 125 75 3 1 123 1 123 2.40e-22 88.2
Msa0179780 MtrunA17_Chr4g0040291 30.380 158 106 1 22 175 22 179 3.96e-22 88.2
Msa0179780 MtrunA17_Chr8g0335571 36.842 114 67 4 12 123 11 121 5.06e-22 88.6
Msa0179780 MtrunA17_Chr3g0132431 35.664 143 85 5 5 145 4 141 8.65e-22 87.4
Msa0179780 MtrunA17_Chr2g0301431 37.168 113 66 2 9 121 16 123 1.07e-21 85.5
Msa0179780 MtrunA17_Chr3g0132441 41.584 101 56 3 22 122 23 120 1.46e-21 87.0
Msa0179780 MtrunA17_Chr1g0196801 34.091 132 80 4 22 151 20 146 7.82e-21 84.7
Msa0179780 MtrunA17_Chr3g0125761 38.393 112 64 2 10 121 18 124 8.12e-21 83.2
Msa0179780 MtrunA17_Chr7g0253731 35.043 117 72 3 1 116 1 114 1.10e-20 84.0
Msa0179780 MtrunA17_Chr6g0483981 34.524 168 98 6 7 166 7 170 1.28e-20 88.6
Msa0179780 MtrunA17_Chr7g0253641 40.299 134 71 4 7 140 14 138 7.42e-20 83.6
Msa0179780 MtrunA17_Chr8g0380901 32.456 114 72 2 8 121 11 119 8.73e-20 80.5
Msa0179780 MtrunA17_Chr1g0188981 35.976 164 99 4 25 183 29 191 1.42e-19 81.6
Msa0179780 MtrunA17_Chr1g0150131 35.922 103 64 1 43 145 46 146 1.70e-19 81.3
Msa0179780 MtrunA17_Chr6g0459441 36.275 102 61 3 22 122 23 121 3.33e-19 80.5
Msa0179780 MtrunA17_Chr3g0132511 35.833 120 72 4 6 122 5 122 3.60e-19 81.3
Msa0179780 MtrunA17_Chr3g0125791 39.394 99 55 3 22 120 30 123 4.29e-19 79.0
Msa0179780 MtrunA17_Chr1g0211101 33.065 124 76 3 8 126 12 133 4.73e-19 80.1
Msa0179780 MtrunA17_Chr3g0132701 35.833 120 72 4 6 122 5 122 6.30e-19 80.9
Msa0179780 MtrunA17_Chr3g0136871 30.935 139 81 3 43 178 48 174 2.45e-18 78.2
Msa0179780 MtrunA17_Chr2g0324721 30.899 178 106 4 1 161 1 178 2.55e-18 78.6
Msa0179780 MtrunA17_Chr8g0380711 35.514 107 59 4 18 121 21 120 2.56e-18 76.6
Msa0179780 MtrunA17_Chr8g0360371 29.851 134 91 2 4 134 1 134 2.58e-18 80.5
Msa0179780 MtrunA17_Chr4g0062341 31.210 157 97 4 25 177 32 181 6.33e-18 77.4
Msa0179780 MtrunA17_Chr4g0062311 31.210 157 97 4 25 177 32 181 6.33e-18 77.4
Msa0179780 MtrunA17_Chr3g0132411 33.871 124 77 4 1 122 39 159 8.58e-18 76.6
Msa0179780 MtrunA17_Chr4g0039721 35.338 133 76 4 25 149 32 162 9.54e-18 76.6
Msa0179780 MtrunA17_Chr4g0041811 33.628 113 73 1 13 123 20 132 1.96e-17 76.6
Msa0179780 MtrunA17_Chr6g0458331 38.235 102 58 2 25 123 37 136 2.49e-17 75.5
Msa0179780 MtrunA17_Chr6g0481491 34.694 98 61 2 25 122 28 122 7.84e-17 75.1
Msa0179780 MtrunA17_Chr7g0253631 38.636 132 71 4 7 138 14 135 1.03e-16 75.1
Msa0179780 MtrunA17_Chr4g0064031 34.615 130 77 4 15 139 18 144 1.08e-15 70.9
Msa0179780 MtrunA17_Chr8g0387804 30.709 127 76 3 6 122 3 127 1.50e-15 69.7
Msa0179780 MtrunA17_Chr1g0185541 45.455 66 36 0 57 122 5 70 2.95e-15 67.4
Msa0179780 MtrunA17_Chr6g0453921 30.303 132 83 4 13 142 26 150 6.90e-15 69.3
Msa0179780 MtrunA17_Chr2g0306461 29.310 116 80 2 8 122 11 125 1.49e-14 68.6
Msa0179780 MtrunA17_Chr8g0385021 28.947 114 80 1 8 120 7 120 2.24e-14 69.3
Msa0179780 MtrunA17_Chr2g0324711 29.801 151 105 1 8 157 8 158 3.46e-14 69.3
Msa0179780 MtrunA17_Chr5g0440804 35.043 117 70 4 28 142 33 145 5.54e-14 66.6
Msa0179780 MtrunA17_Chr4g0033131 29.078 141 89 4 1 134 1 137 9.44e-14 65.9
Msa0179780 MtrunA17_Chr4g0033531 29.078 141 89 4 1 134 1 137 9.44e-14 65.9
Msa0179780 MtrunA17_Chr6g0453801 30.769 117 76 3 8 122 18 131 1.44e-13 65.5
Msa0179780 MtrunA17_Chr6g0453791 30.233 129 82 5 24 150 30 152 3.09e-13 64.7
Msa0179780 MtrunA17_Chr2g0289741 30.769 117 77 3 8 122 9 123 4.73e-13 63.9
Msa0179780 MtrunA17_Chr2g0317231 30.189 106 74 0 20 125 20 125 1.18e-12 65.5
Msa0179780 MtrunA17_Chr8g0385031 29.940 167 103 3 8 161 7 172 2.47e-12 63.9
Msa0179780 MtrunA17_Chr2g0319611 26.400 125 88 2 3 123 4 128 3.34e-12 63.2
Msa0179780 MtrunA17_Chr3g0144921 27.333 150 92 6 4 146 3 142 1.07e-11 60.5
Msa0179780 MtrunA17_Chr8g0385041 30.464 151 84 5 24 157 24 170 2.49e-11 61.2
Msa0179780 MtrunA17_Chr5g0440711 29.167 144 93 5 4 142 6 145 5.64e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0179780 AT5G26330.1 58.182 165 68 1 10 174 9 172 6.47e-65 198
Msa0179780 AT2G31050.1 43.411 129 71 2 10 137 13 140 2.17e-34 120
Msa0179780 AT2G26720.1 38.298 141 82 2 1 137 1 140 2.46e-34 120
Msa0179780 AT2G32300.1 34.337 166 94 5 1 165 1 152 1.23e-30 112
Msa0179780 AT2G32300.2 34.337 166 94 5 1 165 31 182 3.92e-30 112
Msa0179780 AT3G27200.1 32.432 111 71 3 14 123 15 122 2.75e-23 91.3
Msa0179780 AT5G07475.1 34.839 155 87 5 14 168 19 159 2.43e-22 89.4
Msa0179780 AT3G17675.1 41.667 96 54 1 27 122 34 127 3.10e-22 87.4
Msa0179780 AT4G28365.1 30.508 177 107 4 1 161 1 177 1.07e-21 87.8
Msa0179780 AT4G31840.1 32.051 156 94 4 28 180 31 177 1.28e-21 87.0
Msa0179780 AT3G60270.1 37.864 103 61 2 21 123 22 121 3.72e-20 83.6
Msa0179780 AT3G53330.1 35.238 105 67 1 19 122 182 286 1.00e-19 84.7
Msa0179780 AT3G53330.1 39.080 87 47 3 7 92 8 89 2.38e-13 67.4
Msa0179780 AT1G45063.2 40.000 100 59 1 24 122 148 247 1.08e-19 84.0
Msa0179780 AT1G45063.2 32.031 128 81 3 1 123 1 127 1.53e-17 78.2
Msa0179780 AT1G45063.1 40.000 100 59 1 24 122 148 247 1.53e-19 85.1
Msa0179780 AT1G45063.1 32.031 128 81 3 1 123 1 127 2.30e-17 79.0
Msa0179780 AT5G53870.2 30.114 176 109 4 1 165 1 173 2.37e-19 84.3
Msa0179780 AT2G02850.1 31.858 113 72 2 8 120 19 126 3.44e-19 79.3
Msa0179780 AT3G20570.1 29.091 165 106 3 9 163 15 178 4.07e-19 81.3
Msa0179780 AT5G53870.1 30.114 176 109 4 1 165 1 173 4.39e-19 83.6
Msa0179780 AT2G25060.1 33.588 131 81 3 10 134 14 144 7.33e-19 80.1
Msa0179780 AT5G20230.1 33.858 127 79 2 25 150 25 147 1.71e-18 79.3
Msa0179780 AT3G60280.1 39.806 103 59 2 21 123 20 119 1.98e-18 79.7
Msa0179780 AT1G64640.1 29.341 167 92 4 4 161 6 155 3.67e-18 78.6
Msa0179780 AT1G72230.1 30.714 140 92 2 40 175 36 174 9.92e-18 77.0
Msa0179780 AT4G30590.1 31.538 130 81 4 1 123 1 129 1.14e-17 77.0
Msa0179780 AT4G27520.1 31.034 145 93 3 9 146 15 159 1.13e-16 77.0
Msa0179780 AT5G57920.1 31.579 95 61 1 43 133 44 138 2.34e-16 73.6
Msa0179780 AT5G15350.1 33.835 133 80 4 7 137 10 136 2.62e-16 73.2
Msa0179780 AT1G79800.1 30.882 136 85 3 9 137 18 151 4.01e-16 73.2
Msa0179780 AT5G57920.2 31.579 95 61 1 43 133 43 137 4.68e-16 72.8
Msa0179780 AT5G57920.3 30.357 112 72 2 28 133 59 170 9.74e-16 72.4
Msa0179780 AT2G44790.1 37.634 93 53 3 33 123 36 125 1.38e-15 72.0
Msa0179780 AT3G18590.1 34.286 105 59 5 25 123 27 127 4.36e-15 70.1
Msa0179780 AT4G32490.1 28.402 169 98 4 8 158 12 175 5.50e-15 70.5
Msa0179780 AT5G25090.1 33.333 81 54 0 43 123 47 127 5.81e-15 69.7
Msa0179780 AT2G23990.1 36.250 80 50 1 44 122 49 128 9.67e-15 69.7
Msa0179780 AT1G22480.1 31.217 189 106 7 1 183 1 171 1.18e-14 68.9
Msa0179780 AT5G14345.1 37.255 102 58 3 41 141 35 131 3.71e-14 66.6
Msa0179780 AT1G48940.1 31.538 130 77 6 5 126 4 129 1.11e-13 66.2
Msa0179780 AT4G01380.1 28.402 169 82 7 23 180 64 204 1.75e-13 66.2
Msa0179780 AT4G12880.1 33.010 103 64 3 22 122 26 125 1.09e-12 62.8
Msa0179780 AT2G27035.1 25.161 155 104 5 2 155 8 151 1.77e-11 60.1
Msa0179780 AT1G17800.1 29.167 120 78 4 8 123 21 137 5.88e-11 58.2

Find 41 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCATGATTTGTTATCTTTA+TGG 0.100250 1_4:-67483307 None:intergenic
AAATGTGATCATATTATTAT+AGG 0.226490 1_4:-67483754 None:intergenic
TTGTAATTTGTTTACTAGTT+TGG 0.295248 1_4:+67483612 Msa0179780:three_prime_UTR
CTAGCTATATATGCTAAATA+AGG 0.322395 1_4:+67483584 Msa0179780:exon
TGTTTACTAGTTTGGTTTGT+GGG 0.329596 1_4:+67483620 Msa0179780:three_prime_UTR
TCTGTAATTCCTTTACTTGC+TGG 0.332337 1_4:+67483675 Msa0179780:three_prime_UTR
GTCACCAAGTTGGAAGTTCT+TGG 0.351314 1_4:-67482391 None:intergenic
ATCATCTTCACCTTCAGCAT+TGG 0.417122 1_4:+67483428 Msa0179780:CDS
TTGTTTACTAGTTTGGTTTG+TGG 0.424475 1_4:+67483619 Msa0179780:three_prime_UTR
GAGTCTATAGCCAATGGCTT+TGG 0.433150 1_4:+67482239 Msa0179780:exon
TCATATTATTATAGGACAAC+TGG 0.437115 1_4:-67483746 None:intergenic
CCAATTGCAACATTCACTAC+TGG 0.482441 1_4:+67483276 Msa0179780:CDS
TTCAGTGGTGTAGCATTACT+AGG 0.484549 1_4:-67483501 None:intergenic
TTCATATGCAGCTGTATACA+AGG 0.487437 1_4:+67482308 Msa0179780:CDS
CCAGTAGTGAATGTTGCAAT+TGG 0.487702 1_4:-67483276 None:intergenic
AGGTTGGTGATTCTGCTGGC+TGG 0.497516 1_4:+67482328 Msa0179780:CDS
TACAAGGTTGGTGATTCTGC+TGG 0.511253 1_4:+67482324 Msa0179780:CDS
ACCATAAAGATAACAAATCA+TGG 0.515142 1_4:+67483306 Msa0179780:CDS
GCTCTTTCTACCAAAGCCAT+TGG 0.517346 1_4:-67482249 None:intergenic
TGTCCTGCTTGACAGTGTCC+AGG 0.520727 1_4:-67483354 None:intergenic
GGCTTTGGTAGAAAGAGCTA+TGG 0.525531 1_4:+67482254 Msa0179780:CDS
AGGAGAAGGTGCAGGTACAT+TGG 0.525671 1_4:-67483481 None:intergenic
GCTGGAACTGTAGCTTCAGC+AGG 0.528803 1_4:-67483456 None:intergenic
TAATTCTATCCAGCAAGTAA+AGG 0.554771 1_4:-67483684 None:intergenic
GCAGGAGAAGCCAATGCTGA+AGG 0.557674 1_4:-67483438 None:intergenic
GTACCTGGACACTGTCAAGC+AGG 0.560336 1_4:+67483351 Msa0179780:CDS
TATGCAGCTGTATACAAGGT+TGG 0.562762 1_4:+67482312 Msa0179780:CDS
TACTAATAGTGTCACCAAGT+TGG 0.563363 1_4:-67482401 None:intergenic
GCTACCAAGAACTTCCAACT+TGG 0.573625 1_4:+67482387 Msa0179780:CDS
GGTGCAGGTACATTGGAAGC+TGG 0.574590 1_4:-67483474 None:intergenic
CTTGCAGAGTCTATAGCCAA+TGG 0.587150 1_4:+67482233 Msa0179780:exon
AATGCTGAAGGTGAAGATGA+TGG 0.589576 1_4:-67483426 None:intergenic
TTTAAAGCAATACATTTCAG+TGG 0.593570 1_4:-67483516 None:intergenic
GGTGTAGCATTACTAGGAGA+AGG 0.597398 1_4:-67483495 None:intergenic
ATTAACAGCAAGAAATTGCA+TGG 0.600755 1_4:-67483559 None:intergenic
ACACTGTCAAGCAGGACAGA+AGG 0.613934 1_4:+67483359 Msa0179780:CDS
TCTGCTGGCTGGACTACACT+TGG 0.619967 1_4:+67482339 Msa0179780:CDS
GAAGGTGAAGATGATGGAGC+AGG 0.628782 1_4:-67483420 None:intergenic
TGCATTGCATGACTTGTACA+TGG 0.629046 1_4:-67483247 None:intergenic
GTGTCACTCGCATCACATTG+TGG 0.630592 1_4:-67483221 None:intergenic
GCATTACTAGGAGAAGGTGC+AGG 0.651848 1_4:-67483489 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTAAAATAATGTTAATTT+TGG - chr1_4:67482546-67482565 None:intergenic 10.0%
!!! TTAAATTAAGCATTTTTTTA+AGG + chr1_4:67482748-67482767 Msa0179780:intron 10.0%
!!! TAAATTAAGCATTTTTTTAA+GGG + chr1_4:67482749-67482768 Msa0179780:intron 10.0%
!!! TTGTAAAATAATGTTAATTT+TGG - chr1_4:67482546-67482565 None:intergenic 10.0%
!!! TTAAATTAAGCATTTTTTTA+AGG + chr1_4:67482748-67482767 Msa0179780:intron 10.0%
!!! TAAATTAAGCATTTTTTTAA+GGG + chr1_4:67482749-67482768 Msa0179780:intron 10.0%
!! ATTTACTAATACAAAAAACA+AGG + chr1_4:67482200-67482219 Msa0179780:five_prime_UTR 15.0%
!!! AACATTATTTTACAATTCTA+TGG + chr1_4:67482551-67482570 Msa0179780:intron 15.0%
!! AAATGTGATCATATTATTAT+AGG - chr1_4:67483757-67483776 None:intergenic 15.0%
!! ATTTACTAATACAAAAAACA+AGG + chr1_4:67482200-67482219 Msa0179780:five_prime_UTR 15.0%
!!! AACATTATTTTACAATTCTA+TGG + chr1_4:67482551-67482570 Msa0179780:intron 15.0%
!! AAATGTGATCATATTATTAT+AGG - chr1_4:67483757-67483776 None:intergenic 15.0%
!!! TAACATTGACTACAAAAAAT+GGG + chr1_4:67482362-67482381 Msa0179780:CDS 20.0%
!! TATTTCTTCAACAAATTAGA+CGG - chr1_4:67482830-67482849 None:intergenic 20.0%
!! AACTCAAAAAAAAAATTGAG+AGG + chr1_4:67483126-67483145 Msa0179780:intron 20.0%
!!! TTGTAATTTGTTTACTAGTT+TGG + chr1_4:67483612-67483631 Msa0179780:three_prime_UTR 20.0%
!!! TAACATTGACTACAAAAAAT+GGG + chr1_4:67482362-67482381 Msa0179780:CDS 20.0%
!! TATTTCTTCAACAAATTAGA+CGG - chr1_4:67482830-67482849 None:intergenic 20.0%
!! AACTCAAAAAAAAAATTGAG+AGG + chr1_4:67483126-67483145 Msa0179780:intron 20.0%
!!! TTGTAATTTGTTTACTAGTT+TGG + chr1_4:67483612-67483631 Msa0179780:three_prime_UTR 20.0%
!!! AGTAAATTTCTTTTTTGCTG+AGG - chr1_4:67482187-67482206 None:intergenic 25.0%
! TAATACAAAAAACAAGGAGA+AGG + chr1_4:67482206-67482225 Msa0179780:five_prime_UTR 25.0%
!!! TATGGTTTTGTCTATAATGA+TGG + chr1_4:67482272-67482291 Msa0179780:CDS 25.0%
!! GTAACATTGACTACAAAAAA+TGG + chr1_4:67482361-67482380 Msa0179780:CDS 25.0%
! GAACAAATTAAACATGCAAT+TGG - chr1_4:67482437-67482456 None:intergenic 25.0%
!! TTTTCTAACATTGTCCAATT+TGG + chr1_4:67482580-67482599 Msa0179780:intron 25.0%
! ATGAAAACAAAAAGTTGTGT+AGG - chr1_4:67482712-67482731 None:intergenic 25.0%
!!! TTTTTTAAGGGAAACGTTAA+CGG + chr1_4:67482761-67482780 Msa0179780:intron 25.0%
!! TGTTGAAGAAATAGATCTTT+TGG + chr1_4:67482837-67482856 Msa0179780:intron 25.0%
!! ATCATGAAATTGATTTATCC+GGG - chr1_4:67482934-67482953 None:intergenic 25.0%
!! CATCATGAAATTGATTTATC+CGG - chr1_4:67482935-67482954 None:intergenic 25.0%
! ACCATAAAGATAACAAATCA+TGG + chr1_4:67483306-67483325 Msa0179780:CDS 25.0%
!!! ACCATGATTTGTTATCTTTA+TGG - chr1_4:67483310-67483329 None:intergenic 25.0%
!! TTTAAAGCAATACATTTCAG+TGG - chr1_4:67483519-67483538 None:intergenic 25.0%
!!! TATTGCTTTAAAAATGATGC+TGG + chr1_4:67483527-67483546 Msa0179780:CDS 25.0%
! CTAGCTATATATGCTAAATA+AGG + chr1_4:67483584-67483603 Msa0179780:exon 25.0%
! AGAAGAAGTAAAAACCAAAA+AGG - chr1_4:67483646-67483665 None:intergenic 25.0%
!!! TTGGTTTTTACTTCTTCTAT+TGG + chr1_4:67483648-67483667 Msa0179780:three_prime_UTR 25.0%
! TCATATTATTATAGGACAAC+TGG - chr1_4:67483749-67483768 None:intergenic 25.0%
!!! AGTAAATTTCTTTTTTGCTG+AGG - chr1_4:67482187-67482206 None:intergenic 25.0%
! TAATACAAAAAACAAGGAGA+AGG + chr1_4:67482206-67482225 Msa0179780:five_prime_UTR 25.0%
!!! TATGGTTTTGTCTATAATGA+TGG + chr1_4:67482272-67482291 Msa0179780:CDS 25.0%
!! GTAACATTGACTACAAAAAA+TGG + chr1_4:67482361-67482380 Msa0179780:CDS 25.0%
! GAACAAATTAAACATGCAAT+TGG - chr1_4:67482437-67482456 None:intergenic 25.0%
!! TTTTCTAACATTGTCCAATT+TGG + chr1_4:67482580-67482599 Msa0179780:intron 25.0%
! ATGAAAACAAAAAGTTGTGT+AGG - chr1_4:67482712-67482731 None:intergenic 25.0%
!!! TTTTTTAAGGGAAACGTTAA+CGG + chr1_4:67482761-67482780 Msa0179780:intron 25.0%
!! TGTTGAAGAAATAGATCTTT+TGG + chr1_4:67482837-67482856 Msa0179780:intron 25.0%
!! ATCATGAAATTGATTTATCC+GGG - chr1_4:67482934-67482953 None:intergenic 25.0%
!! CATCATGAAATTGATTTATC+CGG - chr1_4:67482935-67482954 None:intergenic 25.0%
! ACCATAAAGATAACAAATCA+TGG + chr1_4:67483306-67483325 Msa0179780:CDS 25.0%
!!! ACCATGATTTGTTATCTTTA+TGG - chr1_4:67483310-67483329 None:intergenic 25.0%
!! TTTAAAGCAATACATTTCAG+TGG - chr1_4:67483519-67483538 None:intergenic 25.0%
!!! TATTGCTTTAAAAATGATGC+TGG + chr1_4:67483527-67483546 Msa0179780:CDS 25.0%
! CTAGCTATATATGCTAAATA+AGG + chr1_4:67483584-67483603 Msa0179780:exon 25.0%
! AGAAGAAGTAAAAACCAAAA+AGG - chr1_4:67483646-67483665 None:intergenic 25.0%
!!! TTGGTTTTTACTTCTTCTAT+TGG + chr1_4:67483648-67483667 Msa0179780:three_prime_UTR 25.0%
! TCATATTATTATAGGACAAC+TGG - chr1_4:67483749-67483768 None:intergenic 25.0%
TTAAACATGCAATTGGAGAA+AGG - chr1_4:67482430-67482449 None:intergenic 30.0%
ATTGGACAATGTTAGAAAAG+AGG - chr1_4:67482579-67482598 None:intergenic 30.0%
! TGAGTTTTTCTGTACCAAAT+TGG - chr1_4:67482597-67482616 None:intergenic 30.0%
!! TGTAGGTCTTAATTAGTCTT+GGG - chr1_4:67482695-67482714 None:intergenic 30.0%
!! TTTTTAAGGGAAACGTTAAC+GGG + chr1_4:67482762-67482781 Msa0179780:intron 30.0%
!!! TAAAAATGATGCTGGCTTTT+TGG + chr1_4:67483535-67483554 Msa0179780:CDS 30.0%
!!! AAAAATGATGCTGGCTTTTT+GGG + chr1_4:67483536-67483555 Msa0179780:CDS 30.0%
ATTAACAGCAAGAAATTGCA+TGG - chr1_4:67483562-67483581 None:intergenic 30.0%
!! TTGTTTACTAGTTTGGTTTG+TGG + chr1_4:67483619-67483638 Msa0179780:three_prime_UTR 30.0%
!! TGTTTACTAGTTTGGTTTGT+GGG + chr1_4:67483620-67483639 Msa0179780:three_prime_UTR 30.0%
TAATTCTATCCAGCAAGTAA+AGG - chr1_4:67483687-67483706 None:intergenic 30.0%
TTAAACATGCAATTGGAGAA+AGG - chr1_4:67482430-67482449 None:intergenic 30.0%
ATTGGACAATGTTAGAAAAG+AGG - chr1_4:67482579-67482598 None:intergenic 30.0%
! TGAGTTTTTCTGTACCAAAT+TGG - chr1_4:67482597-67482616 None:intergenic 30.0%
!! TGTAGGTCTTAATTAGTCTT+GGG - chr1_4:67482695-67482714 None:intergenic 30.0%
!! TTTTTAAGGGAAACGTTAAC+GGG + chr1_4:67482762-67482781 Msa0179780:intron 30.0%
!!! TAAAAATGATGCTGGCTTTT+TGG + chr1_4:67483535-67483554 Msa0179780:CDS 30.0%
!!! AAAAATGATGCTGGCTTTTT+GGG + chr1_4:67483536-67483555 Msa0179780:CDS 30.0%
ATTAACAGCAAGAAATTGCA+TGG - chr1_4:67483562-67483581 None:intergenic 30.0%
!! TTGTTTACTAGTTTGGTTTG+TGG + chr1_4:67483619-67483638 Msa0179780:three_prime_UTR 30.0%
!! TGTTTACTAGTTTGGTTTGT+GGG + chr1_4:67483620-67483639 Msa0179780:three_prime_UTR 30.0%
TAATTCTATCCAGCAAGTAA+AGG - chr1_4:67483687-67483706 None:intergenic 30.0%
!!! TTTCTTTTTTGCTGAGGTTG+AGG - chr1_4:67482181-67482200 None:intergenic 35.0%
TTCATATGCAGCTGTATACA+AGG + chr1_4:67482308-67482327 Msa0179780:CDS 35.0%
TACTAATAGTGTCACCAAGT+TGG - chr1_4:67482404-67482423 None:intergenic 35.0%
!!! GTGTTCACATTTTCTGAAGT+TGG + chr1_4:67482494-67482513 Msa0179780:intron 35.0%
!!! CACATTTTCTGAAGTTGGAA+AGG + chr1_4:67482499-67482518 Msa0179780:intron 35.0%
CTTGAAGCAAAAACATGGAA+AGG - chr1_4:67482627-67482646 None:intergenic 35.0%
!! CTTTCCATGTTTTTGCTTCA+AGG + chr1_4:67482625-67482644 Msa0179780:intron 35.0%
CATGAAAGATAGTGCAATAG+TGG - chr1_4:67482656-67482675 None:intergenic 35.0%
CACTATTGCACTATCTTTCA+TGG + chr1_4:67482654-67482673 Msa0179780:intron 35.0%
! GTGTAGGTCTTAATTAGTCT+TGG - chr1_4:67482696-67482715 None:intergenic 35.0%
GATCTGAATTCGAATTATCC+CGG + chr1_4:67482913-67482932 Msa0179780:intron 35.0%
TAAGGAAATGCAAGTGAAAG+TGG - chr1_4:67482982-67483001 None:intergenic 35.0%
TGCATTTCCTTATTGTTCTG+TGG + chr1_4:67482990-67483009 Msa0179780:intron 35.0%
TCTGTAATTCCTTTACTTGC+TGG + chr1_4:67483675-67483694 Msa0179780:three_prime_UTR 35.0%
!!! TTTCTTTTTTGCTGAGGTTG+AGG - chr1_4:67482181-67482200 None:intergenic 35.0%
TTCATATGCAGCTGTATACA+AGG + chr1_4:67482308-67482327 Msa0179780:CDS 35.0%
TACTAATAGTGTCACCAAGT+TGG - chr1_4:67482404-67482423 None:intergenic 35.0%
!!! GTGTTCACATTTTCTGAAGT+TGG + chr1_4:67482494-67482513 Msa0179780:intron 35.0%
!!! CACATTTTCTGAAGTTGGAA+AGG + chr1_4:67482499-67482518 Msa0179780:intron 35.0%
CTTGAAGCAAAAACATGGAA+AGG - chr1_4:67482627-67482646 None:intergenic 35.0%
!! CTTTCCATGTTTTTGCTTCA+AGG + chr1_4:67482625-67482644 Msa0179780:intron 35.0%
CATGAAAGATAGTGCAATAG+TGG - chr1_4:67482656-67482675 None:intergenic 35.0%
CACTATTGCACTATCTTTCA+TGG + chr1_4:67482654-67482673 Msa0179780:intron 35.0%
! GTGTAGGTCTTAATTAGTCT+TGG - chr1_4:67482696-67482715 None:intergenic 35.0%
GATCTGAATTCGAATTATCC+CGG + chr1_4:67482913-67482932 Msa0179780:intron 35.0%
TAAGGAAATGCAAGTGAAAG+TGG - chr1_4:67482982-67483001 None:intergenic 35.0%
TGCATTTCCTTATTGTTCTG+TGG + chr1_4:67482990-67483009 Msa0179780:intron 35.0%
TCTGTAATTCCTTTACTTGC+TGG + chr1_4:67483675-67483694 Msa0179780:three_prime_UTR 35.0%
TATGCAGCTGTATACAAGGT+TGG + chr1_4:67482312-67482331 Msa0179780:CDS 40.0%
AAGTTGGAAAGGTCCTTTCA+AGG + chr1_4:67482510-67482529 Msa0179780:intron 40.0%
AGTTGGAAAGGTCCTTTCAA+GGG + chr1_4:67482511-67482530 Msa0179780:intron 40.0%
GAAGCAAAAACATGGAAAGG+AGG - chr1_4:67482624-67482643 None:intergenic 40.0%
! GTAGCCTTGAAGCAAAAACA+TGG - chr1_4:67482632-67482651 None:intergenic 40.0%
TTGCACTATCTTTCATGGCA+TGG + chr1_4:67482659-67482678 Msa0179780:intron 40.0%
GAAATGCAAGTGAAAGTGGT+GGG - chr1_4:67482978-67482997 None:intergenic 40.0%
! CAAAGCACCACAGAACAATA+AGG - chr1_4:67483000-67483019 None:intergenic 40.0%
CAACATGCAATGACTTTGTC+AGG + chr1_4:67483037-67483056 Msa0179780:intron 40.0%
AACCATGCTCTCGATGATTT+AGG - chr1_4:67483067-67483086 None:intergenic 40.0%
! TGCATTGCATGACTTGTACA+TGG - chr1_4:67483250-67483269 None:intergenic 40.0%
CCAGTAGTGAATGTTGCAAT+TGG - chr1_4:67483279-67483298 None:intergenic 40.0%
CCAATTGCAACATTCACTAC+TGG + chr1_4:67483276-67483295 Msa0179780:CDS 40.0%
! GGTCATCACTTCTTCTTTTG+TGG + chr1_4:67483327-67483346 Msa0179780:CDS 40.0%
! TTCTTCTTTTGTGGTGTACC+TGG + chr1_4:67483336-67483355 Msa0179780:CDS 40.0%
AATGCTGAAGGTGAAGATGA+TGG - chr1_4:67483429-67483448 None:intergenic 40.0%
ATCATCTTCACCTTCAGCAT+TGG + chr1_4:67483428-67483447 Msa0179780:CDS 40.0%
TTCAGTGGTGTAGCATTACT+AGG - chr1_4:67483504-67483523 None:intergenic 40.0%
TATGCAGCTGTATACAAGGT+TGG + chr1_4:67482312-67482331 Msa0179780:CDS 40.0%
AAGTTGGAAAGGTCCTTTCA+AGG + chr1_4:67482510-67482529 Msa0179780:intron 40.0%
AGTTGGAAAGGTCCTTTCAA+GGG + chr1_4:67482511-67482530 Msa0179780:intron 40.0%
GAAGCAAAAACATGGAAAGG+AGG - chr1_4:67482624-67482643 None:intergenic 40.0%
! GTAGCCTTGAAGCAAAAACA+TGG - chr1_4:67482632-67482651 None:intergenic 40.0%
TTGCACTATCTTTCATGGCA+TGG + chr1_4:67482659-67482678 Msa0179780:intron 40.0%
GAAATGCAAGTGAAAGTGGT+GGG - chr1_4:67482978-67482997 None:intergenic 40.0%
! CAAAGCACCACAGAACAATA+AGG - chr1_4:67483000-67483019 None:intergenic 40.0%
CAACATGCAATGACTTTGTC+AGG + chr1_4:67483037-67483056 Msa0179780:intron 40.0%
AACCATGCTCTCGATGATTT+AGG - chr1_4:67483067-67483086 None:intergenic 40.0%
! TGCATTGCATGACTTGTACA+TGG - chr1_4:67483250-67483269 None:intergenic 40.0%
CCAGTAGTGAATGTTGCAAT+TGG - chr1_4:67483279-67483298 None:intergenic 40.0%
CCAATTGCAACATTCACTAC+TGG + chr1_4:67483276-67483295 Msa0179780:CDS 40.0%
! GGTCATCACTTCTTCTTTTG+TGG + chr1_4:67483327-67483346 Msa0179780:CDS 40.0%
! TTCTTCTTTTGTGGTGTACC+TGG + chr1_4:67483336-67483355 Msa0179780:CDS 40.0%
AATGCTGAAGGTGAAGATGA+TGG - chr1_4:67483429-67483448 None:intergenic 40.0%
ATCATCTTCACCTTCAGCAT+TGG + chr1_4:67483428-67483447 Msa0179780:CDS 40.0%
TTCAGTGGTGTAGCATTACT+AGG - chr1_4:67483504-67483523 None:intergenic 40.0%
CTTGCAGAGTCTATAGCCAA+TGG + chr1_4:67482233-67482252 Msa0179780:exon 45.0%
GAGTCTATAGCCAATGGCTT+TGG + chr1_4:67482239-67482258 Msa0179780:exon 45.0%
GCTCTTTCTACCAAAGCCAT+TGG - chr1_4:67482252-67482271 None:intergenic 45.0%
! GGCTTTGGTAGAAAGAGCTA+TGG + chr1_4:67482254-67482273 Msa0179780:CDS 45.0%
!! TACAAGGTTGGTGATTCTGC+TGG + chr1_4:67482324-67482343 Msa0179780:CDS 45.0%
GCTACCAAGAACTTCCAACT+TGG + chr1_4:67482387-67482406 Msa0179780:CDS 45.0%
! GTCACCAAGTTGGAAGTTCT+TGG - chr1_4:67482394-67482413 None:intergenic 45.0%
TGGTTGTCTTCTCCCTTGAA+AGG - chr1_4:67482526-67482545 None:intergenic 45.0%
GGAAATGCAAGTGAAAGTGG+TGG - chr1_4:67482979-67482998 None:intergenic 45.0%
CATGCAATGACTTTGTCAGG+TGG + chr1_4:67483040-67483059 Msa0179780:intron 45.0%
GTCCTAAATCATCGAGAGCA+TGG + chr1_4:67483062-67483081 Msa0179780:intron 45.0%
GGTGTAGCATTACTAGGAGA+AGG - chr1_4:67483498-67483517 None:intergenic 45.0%
!!! GTTTGGTTTGTGGGCCTTTT+TGG + chr1_4:67483629-67483648 Msa0179780:three_prime_UTR 45.0%
CTTGCAGAGTCTATAGCCAA+TGG + chr1_4:67482233-67482252 Msa0179780:exon 45.0%
GAGTCTATAGCCAATGGCTT+TGG + chr1_4:67482239-67482258 Msa0179780:exon 45.0%
GCTCTTTCTACCAAAGCCAT+TGG - chr1_4:67482252-67482271 None:intergenic 45.0%
! GGCTTTGGTAGAAAGAGCTA+TGG + chr1_4:67482254-67482273 Msa0179780:CDS 45.0%
!! TACAAGGTTGGTGATTCTGC+TGG + chr1_4:67482324-67482343 Msa0179780:CDS 45.0%
GCTACCAAGAACTTCCAACT+TGG + chr1_4:67482387-67482406 Msa0179780:CDS 45.0%
! GTCACCAAGTTGGAAGTTCT+TGG - chr1_4:67482394-67482413 None:intergenic 45.0%
TGGTTGTCTTCTCCCTTGAA+AGG - chr1_4:67482526-67482545 None:intergenic 45.0%
GGAAATGCAAGTGAAAGTGG+TGG - chr1_4:67482979-67482998 None:intergenic 45.0%
CATGCAATGACTTTGTCAGG+TGG + chr1_4:67483040-67483059 Msa0179780:intron 45.0%
GTCCTAAATCATCGAGAGCA+TGG + chr1_4:67483062-67483081 Msa0179780:intron 45.0%
GGTGTAGCATTACTAGGAGA+AGG - chr1_4:67483498-67483517 None:intergenic 45.0%
!!! GTTTGGTTTGTGGGCCTTTT+TGG + chr1_4:67483629-67483648 Msa0179780:three_prime_UTR 45.0%
TGGTCTCGAGCTCGATTGAA+AGG + chr1_4:67482857-67482876 Msa0179780:intron 50.0%
! GTGTCACTCGCATCACATTG+TGG - chr1_4:67483224-67483243 None:intergenic 50.0%
ACACTGTCAAGCAGGACAGA+AGG + chr1_4:67483359-67483378 Msa0179780:CDS 50.0%
!! GAAGGTGAAGATGATGGAGC+AGG - chr1_4:67483423-67483442 None:intergenic 50.0%
! AGGAGAAGGTGCAGGTACAT+TGG - chr1_4:67483484-67483503 None:intergenic 50.0%
! GCATTACTAGGAGAAGGTGC+AGG - chr1_4:67483492-67483511 None:intergenic 50.0%
TGGTCTCGAGCTCGATTGAA+AGG + chr1_4:67482857-67482876 Msa0179780:intron 50.0%
! GTGTCACTCGCATCACATTG+TGG - chr1_4:67483224-67483243 None:intergenic 50.0%
ACACTGTCAAGCAGGACAGA+AGG + chr1_4:67483359-67483378 Msa0179780:CDS 50.0%
!! GAAGGTGAAGATGATGGAGC+AGG - chr1_4:67483423-67483442 None:intergenic 50.0%
! AGGAGAAGGTGCAGGTACAT+TGG - chr1_4:67483484-67483503 None:intergenic 50.0%
! GCATTACTAGGAGAAGGTGC+AGG - chr1_4:67483492-67483511 None:intergenic 50.0%
!! AGGTTGGTGATTCTGCTGGC+TGG + chr1_4:67482328-67482347 Msa0179780:CDS 55.0%
! TCTGCTGGCTGGACTACACT+TGG + chr1_4:67482339-67482358 Msa0179780:CDS 55.0%
GTACCTGGACACTGTCAAGC+AGG + chr1_4:67483351-67483370 Msa0179780:CDS 55.0%
TGTCCTGCTTGACAGTGTCC+AGG - chr1_4:67483357-67483376 None:intergenic 55.0%
GCAGGAGAAGCCAATGCTGA+AGG - chr1_4:67483441-67483460 None:intergenic 55.0%
GCTGGAACTGTAGCTTCAGC+AGG - chr1_4:67483459-67483478 None:intergenic 55.0%
GGTGCAGGTACATTGGAAGC+TGG - chr1_4:67483477-67483496 None:intergenic 55.0%
!! AGGTTGGTGATTCTGCTGGC+TGG + chr1_4:67482328-67482347 Msa0179780:CDS 55.0%
! TCTGCTGGCTGGACTACACT+TGG + chr1_4:67482339-67482358 Msa0179780:CDS 55.0%
GTACCTGGACACTGTCAAGC+AGG + chr1_4:67483351-67483370 Msa0179780:CDS 55.0%
TGTCCTGCTTGACAGTGTCC+AGG - chr1_4:67483357-67483376 None:intergenic 55.0%
GCAGGAGAAGCCAATGCTGA+AGG - chr1_4:67483441-67483460 None:intergenic 55.0%
GCTGGAACTGTAGCTTCAGC+AGG - chr1_4:67483459-67483478 None:intergenic 55.0%
GGTGCAGGTACATTGGAAGC+TGG - chr1_4:67483477-67483496 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 67482114 67483785 67482114 ID=Msa0179780;Name=Msa0179780
chr1_4 mRNA 67482114 67483785 67482114 ID=Msa0179780-mRNA-1;Parent=Msa0179780;Name=Msa0179780-mRNA-1;_AED=0.05;_eAED=0.05;_QI=138|1|1|1|1|1|2|198|185
chr1_4 exon 67482114 67482420 67482114 ID=Msa0179780-mRNA-1:exon:22160;Parent=Msa0179780-mRNA-1
chr1_4 exon 67483199 67483785 67483199 ID=Msa0179780-mRNA-1:exon:22161;Parent=Msa0179780-mRNA-1
chr1_4 five_prime_UTR 67482114 67482251 67482114 ID=Msa0179780-mRNA-1:five_prime_utr;Parent=Msa0179780-mRNA-1
chr1_4 CDS 67482252 67482420 67482252 ID=Msa0179780-mRNA-1:cds;Parent=Msa0179780-mRNA-1
chr1_4 CDS 67483199 67483587 67483199 ID=Msa0179780-mRNA-1:cds;Parent=Msa0179780-mRNA-1
chr1_4 three_prime_UTR 67483588 67483785 67483588 ID=Msa0179780-mRNA-1:three_prime_utr;Parent=Msa0179780-mRNA-1
Gene Sequence

>Msa0179780

ATGGCTTTGGTAGAAAGAGCTATGGTTTTGTCTATAATGATGGTAGCTATGCAGATTTCATATGCAGCTGTATACAAGGTTGGTGATTCTGCTGGCTGGACTACACTTGGTAACATTGACTACAAAAAATGGGCTGCTACCAAGAACTTCCAACTTGGTGACACTATTATATTTGAATATAGTACAAAATTCCACAATGTGATGCGAGTGACACATGCCATGTACAAGTCATGCAATGCATCATCACCAATTGCAACATTCACTACTGGAAATGACACCATAAAGATAACAAATCATGGTCATCACTTCTTCTTTTGTGGTGTACCTGGACACTGTCAAGCAGGACAGAAGGTTGATATTAATGTGCTCAAAGTTTCTGTAGCTGCTTCACCTGCTCCATCATCTTCACCTTCAGCATTGGCTTCTCCTGCTGAAGCTACAGTTCCAGCTTCCAATGTACCTGCACCTTCTCCTAGTAATGCTACACCACTGAAATGTATTGCTTTAAAAATGATGCTGGCTTTTTGGGCCATGCAATTTCTTGCTGTTAATTTCTAG
Protein sequence

>Msa0179780

MALVERAMVLSIMMVAMQISYAAVYKVGDSAGWTTLGNIDYKKWAATKNFQLGDTIIFEYSTKFHNVMRVTHAMYKSCNASSPIATFTTGNDTIKITNHGHHFFFCGVPGHCQAGQKVDINVLKVSVAASPAPSSSPSALASPAEATVPASNVPAPSPSNATPLKCIALKMMLAFWAMQFLAVNF*